Rh4BG300800

Pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002996
Physical Location & Seq
Forward (+)
47193256 .. 47193915
660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG300800.1

Sequence Viewer

Length: 660 bp
ATGGAAAGAACTTATCCCAATAGATGGCCTCGTCCAGTTTGGCATCATCCATGGAAGATGTACAGGGTCATTAATGGCCACTTGGGATCTGCAGGATCGATTGCTTTCGACCCAAGTGATAAATGGTTTTGCACTGGCTCGGCAGATGGCACAATCAAGATATGGGATGTAGCAACCGGAAAGCTACAGCTTACACTGACAGGACATACTCTTCAAATTCGAGGCCTTGCCGTTAGCAGCAAGCATCCCTATATGTTCTCTGCTGGCGATGACAAAGAAGTTAAATGTTGGGACCTTACACAGAATAAGGTCATTCGTTCATATCATGGTCATCTAAGTAGTGTTTACTGCTTGGCAACTCATCCCACTCTTGATATTCTACTCACAGGGACACAGGGGGACGTAATTCAGTATGCCCGGTTTGGGATATTCGTACCCAAACGCATGTTCATGCATTCTTGGGGCACGACAGCACAGTTTGCTCGGTTTTCACTAGACCAATCGATCCCCAAGTTGTCACTGGCTCTCACGACGCAACCATCAAGTTCTGGGACCTTAGAGGTGGAAAAGCAATGTCAACTCTCACACACCATAAGAAATCCGTGCGTGCAATGGCGCCACATCCTAAGGATCAGCATTGTTTTACTTCTGCGTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000375 GO:0000377 GO:0000398 GO:0000974 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0006139 GO:0006355 GO:0006396 GO:0006397 GO:0006508 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009743 GO:0009746 GO:0009749 GO:0009755 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009870 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010154 GO:0010182 GO:0010204 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0031461 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034284 GO:0034641 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046483 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071011 GO:0071013 GO:0071310 GO:0071322 GO:0071495 GO:0071704 GO:0080008 GO:0080090 GO:0080134 GO:0090304 GO:0098542 GO:0099402 GO:1901360 GO:1901564 GO:1901700 GO:1901701 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

219

Amino Acids

24.89

Weight (kDa)

9.61

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 17 - 108 3.6e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 18 - 127 9.4e-18 THOC3 beta-propeller domain
WD40 PF00400 19 - 56 1.8e-09 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 20 - 130 4.5e-14 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd PF24782 20 - 99 7.7e-06 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 20 - 133 1.2e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 22 - 134 3e-22 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 22 - 63 5.3e-06 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 22 - 143 4.3e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 24 - 136 2.7e-25 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 24 - 136 4.5e-15 WDR3 second beta-propeller domain
WDR55 PF24796 27 - 131 6.6e-09 WDR55
Beta-prop_WDR90_POC16_2nd PF23393 33 - 103 1.4e-08 WDR90/POC16, second beta-propeller
WD40_Prp19 PF24814 34 - 135 2.3e-16 Prp19 WD40 domain
Beta-prop_WDR41 PF25178 39 - 132 4.8e-09 WDR41
Beta-prop_SPT8 PF23798 41 - 129 6.6e-06 SPT8 beta propeller
WD40 PF00400 60 - 98 3.6e-07 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 74 - 136 7.8e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 615
AccB7I CCANNNNNTGG 1 cut(s) 24
AclWI GGATC 4 cut(s) 94, 103, 499, 638
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 616
AfaI GTAC 2 cut(s) 62, 435
AfiI CCNNNNNNNGG 2 cut(s) 24, 423
AgsI TTSAA 1 cut(s) 215
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AluBI AGCT 2 cut(s) 184, 190
AluI AGCT 2 cut(s) 184, 190
AlwI GGATC 4 cut(s) 94, 103, 499, 638
AoxI GGCC 3 cut(s) 26, 76, 223
ApeKI GCWGC 1 cut(s) 237
ApoI RAATTY 1 cut(s) 216
AseI ATTAAT 1 cut(s) 72
AspLEI GCGC 1 cut(s) 618
AspS9I GGNCC 2 cut(s) 292, 552
AsuC2I CCSGG 1 cut(s) 418
AvaII GGWCC 2 cut(s) 292, 552
AxyI CCTNAGG 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 467
BalI TGGCCA 1 cut(s) 78
BanI GGYRCC 1 cut(s) 615
BbvI GCAGC 1 cut(s) 249
BccI CCATC 3 cut(s) 18, 140, 547
BceAI ACGGC 1 cut(s) 215
BcnI CCSGG 1 cut(s) 418
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 2 cut(s) 90, 185
BfoI RGCGCY 1 cut(s) 619
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme1390I CCNGG 1 cut(s) 418
Bme18I GGWCC 2 cut(s) 292, 552
BmgT120I GGNCC 2 cut(s) 292, 552
BmiI GGNNCC 3 cut(s) 293, 553, 617
BmrFI CCNGG 1 cut(s) 418
BmsI GCATC 2 cut(s) 52, 253
BpuMI CCSGG 1 cut(s) 418
Bsa29I ATCGAT 2 cut(s) 98, 503
BsaHI GRCGYC 1 cut(s) 616
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 423
Bse1I ACTGG 3 cut(s) 35, 139, 525
Bse21I CCTNAGG 1 cut(s) 626
Bse3DI GCAATG 2 cut(s) 578, 617
BseCI ATCGAT 2 cut(s) 98, 503
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 5 cut(s) 46, 172, 244, 361, 621
BseLI CCNNNNNNNGG 2 cut(s) 24, 423
BseMI GCAATG 2 cut(s) 578, 617
BseNI ACTGG 3 cut(s) 35, 139, 525
BseSI GKGCMC 1 cut(s) 467
BseXI GCAGC 1 cut(s) 249
BshFI GGCC 3 cut(s) 28, 78, 225
BshNI GGYRCC 1 cut(s) 615
BshVI ATCGAT 2 cut(s) 98, 503
BsiSI CCGG 2 cut(s) 177, 418
BslFI GGGAC 4 cut(s) 305, 403, 413, 565
BslI CCNNNNNNNGG 2 cut(s) 24, 423
BsmFI GGGAC 4 cut(s) 305, 403, 413, 565
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 3 cut(s) 28, 78, 225
Bsp1286I GDGCHC 1 cut(s) 467
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 4 cut(s) 86, 95, 504, 630
Bsp19I CCATGG 1 cut(s) 50
BspANI GGCC 3 cut(s) 28, 78, 225
BspDI ATCGAT 2 cut(s) 98, 503
BspLI GGNNCC 3 cut(s) 293, 553, 617
BspMAI CTGCAG 1 cut(s) 94
BspPI GGATC 4 cut(s) 94, 103, 499, 638
BspT107I GGYRCC 1 cut(s) 615
BsrDI GCAATG 2 cut(s) 578, 617
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 3 cut(s) 35, 139, 525
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 4 cut(s) 86, 95, 504, 630
BssNI GRCGYC 1 cut(s) 616
BssT1I CCWWGG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 477
Bst6I CTCTTC 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 616
BstAPI GCANNNNNTGC 1 cut(s) 479
BstAUI TGTACA 1 cut(s) 60
BstC8I GCNNGC 3 cut(s) 242, 265, 608
BstDEI CTNAG 3 cut(s) 335, 556, 626
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 5 cut(s) 46, 172, 244, 361, 621
BstH2I RGCGCY 1 cut(s) 619
BstHHI GCGC 1 cut(s) 618
BstKTI GATC 4 cut(s) 89, 98, 507, 633
BstMBI GATC 4 cut(s) 86, 95, 504, 630
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNSI RCATGY 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 416
BstSFI CTRYAG 2 cut(s) 90, 185
BstSLI GKGCMC 1 cut(s) 467
BstV1I GCAGC 1 cut(s) 249
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
Bsu15I ATCGAT 2 cut(s) 98, 503
Bsu36I CCTNAGG 1 cut(s) 626
BsuRI GGCC 3 cut(s) 28, 78, 225
BsuTUI ATCGAT 2 cut(s) 98, 503
BtgI CCRYGG 1 cut(s) 50
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 5 cut(s) 46, 172, 244, 361, 621
BtsIMutI CAGTG 3 cut(s) 132, 194, 518
Cac8I GCNNGC 3 cut(s) 242, 265, 608
CfoI GCGC 1 cut(s) 618
Cfr13I GGNCC 2 cut(s) 292, 552
ClaI ATCGAT 2 cut(s) 98, 503
CseI GACGC 2 cut(s) 541, 641
Csp6I GTAC 2 cut(s) 61, 434
CviAII CATG 4 cut(s) 51, 326, 445, 451
CviJI RGCY 7 cut(s) 28, 78, 138, 184, 190, 225, 524
CviKI_1 RGCY 7 cut(s) 28, 78, 138, 184, 190, 225, 524
CviQI GTAC 2 cut(s) 61, 434
DdeI CTNAG 3 cut(s) 335, 556, 626
DinI GGCGCC 1 cut(s) 617
DpnI GATC 4 cut(s) 88, 97, 506, 632
DpnII GATC 4 cut(s) 86, 95, 504, 630
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 50
Eco147I AGGCCT 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 292, 552
Eco81I CCTNAGG 1 cut(s) 626
EcoO109I RGGNCCY 2 cut(s) 292, 552
EcoT14I CCWWGG 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 456
EgeI GGCGCC 1 cut(s) 617
EheI GGCGCC 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 4 cut(s) 54, 329, 448, 454
FaqI GGGAC 4 cut(s) 305, 403, 413, 565
FatI CATG 4 cut(s) 50, 325, 444, 450
Fnu4HI GCNGC 1 cut(s) 238
FokI GGATG 5 cut(s) 33, 179, 231, 348, 608
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 1 cut(s) 494
GlaI GCGC 1 cut(s) 617
GluI GCNGC 1 cut(s) 238
HaeII RGCGCY 1 cut(s) 619
HaeIII GGCC 3 cut(s) 28, 78, 225
HapII CCGG 2 cut(s) 177, 418
HgaI GACGC 2 cut(s) 541, 641
HhaI GCGC 1 cut(s) 618
Hin1I GRCGYC 1 cut(s) 616
Hin1II CATG 4 cut(s) 54, 329, 448, 454
Hin6I GCGC 1 cut(s) 616
HinP1I GCGC 1 cut(s) 616
HincII GTYRAC 1 cut(s) 578
HindII GTYRAC 1 cut(s) 578
HpaII CCGG 2 cut(s) 177, 418
Hpy166II GTNNAC 2 cut(s) 346, 578
Hpy188III TCNNGA 3 cut(s) 157, 371, 529
Hpy8I GTNNAC 2 cut(s) 346, 578
Hpy99I CGWCG 1 cut(s) 535
HpyCH4III ACNGT 1 cut(s) 477
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 4 cut(s) 92, 132, 454, 610
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 3 cut(s) 335, 556, 626
HpySE526I ACGT 1 cut(s) 402
Hsp92I GRCGYC 1 cut(s) 616
Hsp92II CATG 4 cut(s) 54, 329, 448, 454
HspAI GCGC 1 cut(s) 616
KasI GGCGCC 1 cut(s) 615
Kzo9I GATC 4 cut(s) 86, 95, 504, 630
Lsp1109I GCAGC 1 cut(s) 249
LweI GCATC 2 cut(s) 52, 253
MaeI CTAG 1 cut(s) 494
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 516
MalI GATC 4 cut(s) 88, 97, 506, 632
MboI GATC 4 cut(s) 86, 95, 504, 630
MboII GAAGA 2 cut(s) 67, 203
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 1 cut(s) 467
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 2 cut(s) 216, 405
MluNI TGGCCA 1 cut(s) 78
Mly113I GGCGCC 1 cut(s) 616
MnlI CCTC 3 cut(s) 39, 215, 553
Mox20I TGGCCA 1 cut(s) 78
Mph1103I ATGCAT 1 cut(s) 456
MscI TGGCCA 1 cut(s) 78
MseI TTAA 2 cut(s) 72, 282
MslI CAYNNNNRTG 1 cut(s) 449
Msp20I TGGCCA 1 cut(s) 78
MspI CCGG 2 cut(s) 177, 418
MspR9I CCNGG 1 cut(s) 418
Mva1269I GAATGC 1 cut(s) 454
MwoI GCNNNNNNNGC 1 cut(s) 479
NarI GGCGCC 1 cut(s) 616
NciI CCSGG 1 cut(s) 418
NcoI CCATGG 1 cut(s) 50
NdeII GATC 4 cut(s) 86, 95, 504, 630
NlaIII CATG 4 cut(s) 54, 329, 448, 454
NlaIV GGNNCC 3 cut(s) 293, 553, 617
NmeAIII GCCGAG 1 cut(s) 119
NmuCI GTSAC 1 cut(s) 516
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 1 cut(s) 448
PceI AGGCCT 1 cut(s) 225
PctI GAATGC 1 cut(s) 454
PflMI CCANNNNNTGG 1 cut(s) 24
PkrI GCNGC 1 cut(s) 239
PluTI GGCGCC 1 cut(s) 619
PpuMI RGGWCCY 2 cut(s) 292, 552
PshBI ATTAAT 1 cut(s) 72
Psp5II RGGWCCY 2 cut(s) 292, 552
PspN4I GGNNCC 3 cut(s) 293, 553, 617
PspPI GGNCC 2 cut(s) 292, 552
PspPPI RGGWCCY 2 cut(s) 292, 552
PstI CTGCAG 1 cut(s) 94
PsuI RGATCY 1 cut(s) 86
RsaI GTAC 2 cut(s) 62, 435
RsaNI GTAC 2 cut(s) 61, 434
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 2 cut(s) 72, 282
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 4 cut(s) 86, 95, 504, 630
Sau96I GGNCC 2 cut(s) 292, 552
ScrFI CCNGG 1 cut(s) 418
SduI GDGCHC 1 cut(s) 467
SetI ASST 7 cut(s) 186, 192, 297, 312, 405, 557, 564
SfaNI GCATC 2 cut(s) 52, 253
SfcI CTRYAG 2 cut(s) 90, 185
SfoI GGCGCC 1 cut(s) 617
SinI GGWCC 2 cut(s) 292, 552
SmiMI CAYNNNNRTG 1 cut(s) 449
Sse9I AATT 2 cut(s) 216, 405
SseBI AGGCCT 1 cut(s) 225
SspDI GGCGCC 1 cut(s) 615
SspMI CTAG 1 cut(s) 494
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 416
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 1 cut(s) 477
TaiI ACGT 1 cut(s) 405
TaqI TCGA 4 cut(s) 98, 108, 220, 503
TasI AATT 2 cut(s) 216, 405
TatI WGTACW 1 cut(s) 60
Tru1I TTAA 2 cut(s) 72, 282
Tru9I TTAA 2 cut(s) 72, 282
TscAI CASTG 3 cut(s) 139, 201, 525
TseFI GTSAC 1 cut(s) 516
TseI GCWGC 1 cut(s) 237
Tsp45I GTSAC 1 cut(s) 516
TspDTI ATGAA 2 cut(s) 309, 439
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 3 cut(s) 139, 201, 525
Van91I CCANNNNNTGG 1 cut(s) 24
VpaK11BI GGWCC 2 cut(s) 292, 552
VspI ATTAAT 1 cut(s) 72
XapI RAATTY 1 cut(s) 216
XceI RCATGY 1 cut(s) 448
XcmI CCANNNNNNNNNTGG 2 cut(s) 120, 517
XspI CTAG 1 cut(s) 494
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.