Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0001729

Physical Location & Seq

Genomic Coordinates Reverse (-)

26669304 .. 26676597

Gene Structure 7294 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g24570.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGACGGAAAGTGAAGGCGACGCCGTCAAAATGCCGAAGCAGAATCGCCGGAGCCACCGCCTTGTTGCCGTCACCGGAGCCACCGCTCTCCTCGCTCTCGCCGTCCACCTCGGAATCACCGCCTTCAACTCTCACCGGAAGAACAGAAACAAGAAAGATCTTCACGGCTCCAAGGTTCGAGTCACTCTATCGGCGTCGGAGATTATCAAATTGGCGGAGCGAGTCATTTCCAAGTCGAAAGAGGTCCACGACGCTGTGGCTTCAGTTCCGCTTGATAAGGTTACGTATATGAATGTTATATCACCTCTTGCTGAATTGGAAGCGCAGCAATTTCCACTTGTTCAGTCTTGCGTGTTTCCTAAGATGGTAACTACTAGTGATGATGTTCGAAAAGCAAGCGCCGAGGCTGAGCGGAGAATCGATGCACATGTTTTGACTTGCAGCATGCGTGAGGATGTGTACCGTGTAGTCAAAGCTTTTGCTATGAGAGGAGAGTGGGTGAATGCTGACGCAAAGCACTATACTCAAGCCTTGATGAGAGACTTTGAACGCAATGGGTTGAATCTTACTTTAACTAAGAGAGAGGAGATGCAGCGTGTCAGAGTCCATATTGATGAGCTAAGCTTGCGCTATATCCAAAATCTACATGAAGATTGTTCTTTCCTTGTCTTCACTGAAACTGAGTTGGCTGGTCTACCACAAGAGTTTCTTAAGAGCTTAGACAAAGCTGACAATGGTAAATTGAAGGTGACCCTGAAAAGTCATCATGTGGTGCCAGTATTAGAACTTTGCGAGGTTGGAACAACTAGGAAGATGGTAGCAGTGGAATATGGGAAGAGGTGTGGAGAAGTCAACCTTTCTATCTTAGAAGATCTGGTTCAACTTCGCCATAAGTTTGCTCGGGTACTCGGCTATTCAAATTATGCTGAATATGCTGTTGATCATAGAATGGCAAAGACACCTGCAAAGGTTTATGCGTTCTTAGAGGACATCTCCAATAGTTTGGCCAATTCAGCTTTCATGGAGCTTTCTATGTTGAAAGATTTGAAGAAAAAAGAGGAAGGAGATTTTCCATTTGGAGTTGAGGATCTACTATACTATGTGAAGAAGGCTGAAGAACAGCAGTTTGATTTTGATTTTGACTTTGGAGCTCTCAAGCAATACTTTCCAGTAAATTTGGTTTTATCTGGGGTCTTTAAAATAGTCCAAGACCTTTTTGGTTTAAGGTTTGAGGAAATTACAGATGCCGAGGTGTGGCATTCTGATGTTTCCGCATATTCAGTTTTCGACCTGAGTTCTGGTGAACTGTTGGGCCATTTCTACCTTGATATGTACACAAGGGAAGGAAAATATAGTCATACGTGTGTGGTGGCTCTTCAAAATGGTGCTTTATCATCCAATGGATCACGTCAGATACCAGTTGCATTATTGATATCTCAATTCCAAAAAGATGTCAGCGGTCAACCTGCATTACTTCGATTTTCTGAAGTTGTGAACCTTTTTCACGAGTTTGGCCATGTGGTCCAACATATTTGCAATCGTGCTTCATTCTCCAAGTTTTCTGGTCTGGGTTTTGATCCAGACTTTGTGGAGGTTCCTGCTCAGGTGCTAGAGAATTGGTGCTATGAAAGCTTCAGTTTGAAGTTGATATCCGGCTTCCATCAGGATATAACAAAGCCCATCAAAGATGAAATGTGCAAATCCATTAAAAGATGGCGATATTCCTTTTCAGCTCTGAAAATGAAGCAACAAATTCTTTATTGTATTTTCGACCAAATCATACATTCTGCTGAAAACGTTGACATAGTTGAGTTATTTAAGCATCTTCATCCAACGATCATGTTAGGTTTACCAGCTTTGGAAGGTGTCAATCCAGCTTCACGGTTTCCATGTAGTGCCATTGGGTATGATGCTTCTTGTTACAGCCGTATTTGGAGTGAGGTCTTCACTGCGGATATATTTGCATCAAAGTTTCATGATAGTTATTTGAACCAGTATGCTGGCATGCAGTTCAGGAACAAGGTATTGGCCCCAGGGGGATCAAAGGAACCGATTGAAGTTTTAACAGGCTTTCTTGGGAGAGAACCATCAACTCAAGCTTTTATCGACAGCAGATCCCATTACAGATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005758 GO:0005829 GO:0005886 GO:0006109 GO:0006111 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009889 GO:0009987 GO:0010675 GO:0010830 GO:0010906 GO:0016020 GO:0016202 GO:0016787 GO:0019222 GO:0019538 GO:0031323 GO:0031967 GO:0031970 GO:0031974 GO:0031975 GO:0033218 GO:0034641 GO:0042277 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043255 GO:0043603 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0048634 GO:0048641 GO:0050789 GO:0050793 GO:0050794 GO:0051147 GO:0051153 GO:0051239 GO:0062012 GO:0065007 GO:0070011 GO:0070012 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:0140096 GO:1901564 GO:1901861 GO:1902809 GO:2000026 GO:2001014

KEGG Pathways

Metabolic & Signaling

ko04614 ko05143 map04614 map05143

Pfam Domains

Protein Families

Peptidase_M3

Protein Analysis

711

Amino Acids

80.49

Weight (kDa)

6.59

Isoelectric Point (pI)

37.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M3	PF01432	263 - 481	2.2e-47	Peptidase family M3
Peptidase_M3	PF01432	472 - 668	5.6e-32	Peptidase family M3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001729)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67690
fragaria_vesca	FvH4_4g24570 FvH4_4g24570 FvH4_4g24570 FvH4_4g24570
malus_domestica	MD02G1151800.v1.1 MD13G1110200.v1.1 MD16G1110100.v1.1 MD16G1110200.v1.1
prunus_persica	Prupe.1G234500_v2.0.a1 Prupe.1G234500_v2.0.a1 Prupe.1G234500_v2.0.a1 Prupe.1G234500_v2.0.a1 Prupe.1G234500_v2.0.a1 Prupe.1G234500_v2.0.a1 Prupe.1G234500_v2.0.a1 Prupe.1G234500_v2.0.a1
pyrus_communis	pycom13g09590 pycom16g09360
rosa_chinensis	RchiOBHm_Chr4g0431491 RchiOBHm_Chr4g0431531 RchiOBHm_Chr4g0444651
rosa_laevigata	RLG00000006910 RLG00000018869 RLG00000036429
rosa_multiflora	Rmu_sc0000543.1_g000041 Rmu_sc0001207.1_g000024
rosa_roxburghii	Rroxscaffold_5G00372940 Rroxscaffold_5G00372960 Rroxscaffold_5G00372990 Rroxscaffold_5G00373020
rosa_rugosa	Rorug04G0250600 Rorug04G0250700
rosa_samantha	Rh2AG013000 Rh2AG278200 Rh4AG306800 Rh4AG307200 Rh4AG307400 Rh4BG314800 Rh4BG315100 Rh4CG330200 Rh4DG310000 Rh4DG310100 Rh4DG421900 Rh5AG518200 Rh5DG435400 Rh6CG219500
rosa_wichuraiana	Rw4G026630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	970
Acc36I	ACCTGC	2 cut(s)	970, 1474
AccB1I	GGYRCC	1 cut(s)	772
AccBSI	CCGCTC	2 cut(s)	86, 412
AccI	GTMKAC	1 cut(s)	694
AciI	CCGC	9 cut(s)	58, 84, 120, 215, 269, 412, 1272, 1458, 1952
AclI	AACGTT	1 cut(s)	1797
AclWI	GGATC	5 cut(s)	1095, 1411, 1571, 2047, 2109
AcoI	YGGCCR	2 cut(s)	1005, 1513
AcsI	RAATTY	2 cut(s)	1174, 1752
AcuI	CTGAAG	4 cut(s)	246, 1134, 1506, 1618
AcyI	GRCGYC	2 cut(s)	21, 194
AfaI	GTAC	3 cut(s)	461, 906, 1334
AfiI	CCNNNNNNNGG	1 cut(s)	1254
AflII	CTTAAG	1 cut(s)	710
AflIII	ACRYGT	2 cut(s)	427, 1361
AhlI	ACTAGT	1 cut(s)	374
AjiI	CACGTC	1 cut(s)	1409
AjnI	CCWGG	1 cut(s)	2032
AjuI	GAANNNNNNNTTGG	4 cut(s)	1001, 1033, 2009, 2041
Alw21I	GWGCWC	1 cut(s)	1153
Alw26I	GTCTC	1 cut(s)	534
AlwI	GGATC	5 cut(s)	1095, 1411, 1571, 2047, 2109
Ama87I	CYCGRG	1 cut(s)	900
AoxI	GGCC	4 cut(s)	1005, 1312, 1513, 2028
ApeKI	GCWGC	3 cut(s)	325, 441, 592
ApoI	RAATTY	2 cut(s)	1174, 1752
AspLEI	GCGC	3 cut(s)	325, 401, 630
AspS9I	GGNCC	4 cut(s)	244, 1312, 1522, 2029
AsuHPI	GGTGA	7 cut(s)	64, 109, 125, 294, 511, 760, 1313
AsuII	TTCGAA	1 cut(s)	388
AvaI	CYCGRG	1 cut(s)	900
AvaII	GGWCC	2 cut(s)	244, 1522
BalI	TGGCCA	2 cut(s)	1007, 1515
BanI	GGYRCC	1 cut(s)	772
BanII	GRGCYC	1 cut(s)	1153
BauI	CACGAG	1 cut(s)	1505
BbsI	GAAGAC	2 cut(s)	661, 1936
Bbv12I	GWGCWC	1 cut(s)	1153
BbvI	GCAGC	3 cut(s)	337, 453, 604
BccI	CCATC	6 cut(s)	358, 808, 1668, 1688, 1707, 2095
BceAI	ACGGC	5 cut(s)	8, 53, 86, 181, 1911
BciT130I	CCWGG	1 cut(s)	2034
BclI	TGATCA	1 cut(s)	940
BcoDI	GTCTC	1 cut(s)	534
BcuI	ACTAGT	1 cut(s)	374
BfaI	CTAG	3 cut(s)	375, 807, 1610
BfoI	RGCGCY	1 cut(s)	402
BfrI	CTTAAG	1 cut(s)	710
BfuAI	ACCTGC	2 cut(s)	970, 1474
BglII	AGATCT	2 cut(s)	157, 871
BisI	GCNGC	3 cut(s)	326, 442, 593
BlpI	GCTNAGC	2 cut(s)	408, 620
BlsI	GCNGC	3 cut(s)	327, 443, 594
Bme1390I	CCNGG	1 cut(s)	2034
Bme18I	GGWCC	2 cut(s)	244, 1522
BmeT110I	CYCGRG	1 cut(s)	900
BmgBI	CACGTC	1 cut(s)	1409
BmgT120I	GGNCC	4 cut(s)	244, 1312, 1522, 2029
BmiI	GGNNCC	7 cut(s)	53, 79, 169, 774, 1596, 2031, 2049
BmrFI	CCNGG	1 cut(s)	2034
BmsI	GCATC	6 cut(s)	412, 579, 1234, 1831, 1900, 1973
BpiI	GAAGAC	2 cut(s)	661, 1936
BplI	GAGNNNNNCTC	2 cut(s)	977, 1009
Bpu10I	CCTNAGC	1 cut(s)	1602
Bpu1102I	GCTNAGC	2 cut(s)	408, 620
Bpu14I	TTCGAA	1 cut(s)	388
BpuEI	CTTGAG	3 cut(s)	510, 1139, 2079
Bsa29I	ATCGAT	1 cut(s)	420
BsaAI	YACGTR	2 cut(s)	285, 1362
BsaHI	GRCGYC	2 cut(s)	21, 194
BsaJI	CCNNGG	6 cut(s)	109, 171, 402, 1248, 2032, 2033
BsaWI	WCCGGW	2 cut(s)	74, 135
BsaXI	ACNNNNNCTCC	2 cut(s)	483, 513
Bsc4I	CCNNNNNNNGG	1 cut(s)	1254
Bse1I	ACTGG	4 cut(s)	776, 1169, 1418, 1993
Bse3DI	GCAATG	1 cut(s)	559
BseBI	CCWGG	1 cut(s)	2034
BseCI	ATCGAT	1 cut(s)	420
BseDI	CCNNGG	6 cut(s)	109, 171, 402, 1248, 2032, 2033
BseGI	GGATG	3 cut(s)	460, 1394, 1828
BseLI	CCNNNNNNNGG	1 cut(s)	1254
BseMI	GCAATG	1 cut(s)	559
BseMII	CTCAG	4 cut(s)	399, 672, 1283, 1616
BseNI	ACTGG	4 cut(s)	776, 1169, 1418, 1993
BseRI	GAGGAG	3 cut(s)	80, 504, 599
BseXI	GCAGC	3 cut(s)	337, 453, 604
BshFI	GGCC	4 cut(s)	1007, 1314, 1515, 2030
BshNI	GGYRCC	1 cut(s)	772
BshVI	ATCGAT	1 cut(s)	420
BsiHKAI	GWGCWC	1 cut(s)	1153
BsiHKCI	CYCGRG	1 cut(s)	900
BsiSI	CCGG	4 cut(s)	49, 75, 136, 1653
BslI	CCNNNNNNNGG	1 cut(s)	1254
BsmAI	GTCTC	1 cut(s)	534
BsmI	GAATGC	2 cut(s)	508, 1258
BsnI	GGCC	4 cut(s)	1007, 1314, 1515, 2030
BsoBI	CYCGRG	1 cut(s)	900
Bsp119I	TTCGAA	1 cut(s)	388
Bsp1286I	GDGCHC	1 cut(s)	1153
Bsp1407I	TGTACA	1 cut(s)	1332
Bsp143I	GATC	9 cut(s)	157, 871, 940, 1087, 1403, 1576, 1836, 2039, 2114
Bsp1720I	GCTNAGC	2 cut(s)	408, 620
BspACI	CCGC	9 cut(s)	58, 84, 120, 215, 269, 412, 1272, 1458, 1952
BspANI	GGCC	4 cut(s)	1007, 1314, 1515, 2030
BspCNI	CTCAG	4 cut(s)	400, 673, 1284, 1615
BspDI	ATCGAT	1 cut(s)	420
BspHI	TCATGA	1 cut(s)	1975
BspLI	GGNNCC	7 cut(s)	53, 79, 169, 774, 1596, 2031, 2049
BspMI	ACCTGC	2 cut(s)	970, 1474
BspPI	GGATC	5 cut(s)	1095, 1411, 1571, 2047, 2109
BspQI	GCTCTTC	1 cut(s)	1380
BspT104I	TTCGAA	1 cut(s)	388
BspT107I	GGYRCC	1 cut(s)	772
BspTI	CTTAAG	1 cut(s)	710
BsrBI	CCGCTC	2 cut(s)	86, 412
BsrDI	GCAATG	1 cut(s)	559
BsrGI	TGTACA	1 cut(s)	1332
BsrI	ACTGG	4 cut(s)	776, 1169, 1418, 1993
BssECI	CCNNGG	6 cut(s)	109, 171, 402, 1248, 2032, 2033
BssMI	GATC	9 cut(s)	157, 871, 940, 1087, 1403, 1576, 1836, 2039, 2114
BssNI	GRCGYC	2 cut(s)	21, 194
BssSI	CACGAG	1 cut(s)	1505
BssT1I	CCWWGG	1 cut(s)	171
Bst2BI	CACGAG	1 cut(s)	1505
Bst2UI	CCWGG	1 cut(s)	2034
Bst4CI	ACNGT	3 cut(s)	464, 1308, 1884
Bst6I	CTCTTC	2 cut(s)	830, 1380
BstACI	GRCGYC	2 cut(s)	21, 194
BstAFI	CTTAAG	1 cut(s)	710
BstAUI	TGTACA	1 cut(s)	1332
BstBAI	YACGTR	2 cut(s)	285, 1362
BstBI	TTCGAA	1 cut(s)	388
BstC8I	GCNNGC	5 cut(s)	397, 446, 626, 2002, 2006
BstEII	GGTNACC	1 cut(s)	748
BstF5I	GGATG	3 cut(s)	460, 1394, 1828
BstH2I	RGCGCY	1 cut(s)	402
BstHHI	GCGC	3 cut(s)	325, 401, 630
BstKTI	GATC	9 cut(s)	160, 874, 943, 1090, 1406, 1579, 1839, 2042, 2117
BstMAI	GTCTC	1 cut(s)	534
BstMBI	GATC	9 cut(s)	157, 871, 940, 1087, 1403, 1576, 1836, 2039, 2114
BstMWI	GCNNNNNNNGC	5 cut(s)	92, 625, 932, 1013, 1629
BstNI	CCWGG	1 cut(s)	2034
BstNSI	RCATGY	3 cut(s)	431, 448, 2008
BstPI	GGTNACC	1 cut(s)	748
BstSCI	CCNGG	1 cut(s)	2032
BstSNI	TACGTA	1 cut(s)	285
BstV1I	GCAGC	3 cut(s)	337, 453, 604
BstV2I	GAAGAC	2 cut(s)	661, 1936
BstX2I	RGATCY	4 cut(s)	157, 871, 1087, 2114
BstXI	CCANNNNNNTGG	2 cut(s)	1003, 2000
BstYI	RGATCY	4 cut(s)	157, 871, 1087, 2114
Bsu15I	ATCGAT	1 cut(s)	420
BsuRI	GGCC	4 cut(s)	1007, 1314, 1515, 2030
BsuTUI	ATCGAT	1 cut(s)	420
BtrI	CACGTC	1 cut(s)	1409
BtsCI	GGATG	3 cut(s)	460, 1394, 1828
BtsI	GCAGTG	2 cut(s)	828, 1947
BtsIMutI	CAGTG	3 cut(s)	672, 828, 1947
BveI	ACCTGC	2 cut(s)	970, 1474
Cac8I	GCNNGC	5 cut(s)	397, 446, 626, 2002, 2006
CciI	TCATGA	1 cut(s)	1975
CfoI	GCGC	3 cut(s)	325, 401, 630
Cfr13I	GGNCC	4 cut(s)	244, 1312, 1522, 2029
ClaI	ATCGAT	1 cut(s)	420
CseI	GACGC	4 cut(s)	29, 183, 260, 518
Csp6I	GTAC	3 cut(s)	460, 905, 1333
CviQI	GTAC	3 cut(s)	460, 905, 1333
DpnI	GATC	9 cut(s)	159, 873, 942, 1089, 1405, 1578, 1838, 2041, 2116
DpnII	GATC	9 cut(s)	157, 871, 940, 1087, 1403, 1576, 1836, 2039, 2114
DraI	TTTAAA	1 cut(s)	1198
EaeI	YGGCCR	2 cut(s)	1005, 1513
Eam1104I	CTCTTC	2 cut(s)	830, 1380
EarI	CTCTTC	2 cut(s)	830, 1380
EciI	GGCGGA	1 cut(s)	230
Ecl136II	GAGCTC	1 cut(s)	1151
Eco105I	TACGTA	1 cut(s)	285
Eco130I	CCWWGG	1 cut(s)	171
Eco24I	GRGCYC	1 cut(s)	1153
Eco32I	GATATC	2 cut(s)	1434, 1650
Eco47I	GGWCC	2 cut(s)	244, 1522
Eco53kI	GAGCTC	1 cut(s)	1151
Eco57I	CTGAAG	4 cut(s)	246, 1134, 1506, 1618
Eco88I	CYCGRG	1 cut(s)	900
Eco91I	GGTNACC	1 cut(s)	748
EcoICRI	GAGCTC	1 cut(s)	1151
EcoO65I	GGTNACC	1 cut(s)	748
EcoRII	CCWGG	1 cut(s)	2032
EcoRV	GATATC	2 cut(s)	1434, 1650
EcoT14I	CCWWGG	1 cut(s)	171
EcoT38I	GRGCYC	1 cut(s)	1153
ErhI	CCWWGG	1 cut(s)	171
FalI	AAGNNNNNCTT	6 cut(s)	693, 725, 840, 872, 1148, 1180
FbaI	TGATCA	1 cut(s)	940
FblI	GTMKAC	1 cut(s)	694
Fnu4HI	GCNGC	3 cut(s)	326, 442, 593
FokI	GGATG	3 cut(s)	467, 1381, 1815
FriOI	GRGCYC	1 cut(s)	1153
Fsp4HI	GCNGC	3 cut(s)	326, 442, 593
FspBI	CTAG	3 cut(s)	375, 807, 1610
GlaI	GCGC	3 cut(s)	324, 400, 629
GluI	GCNGC	3 cut(s)	326, 442, 593
HaeII	RGCGCY	1 cut(s)	402
HaeIII	GGCC	4 cut(s)	1007, 1314, 1515, 2030
HapII	CCGG	4 cut(s)	49, 75, 136, 1653
HgaI	GACGC	4 cut(s)	29, 183, 260, 518
HhaI	GCGC	3 cut(s)	325, 401, 630
Hin1I	GRCGYC	2 cut(s)	21, 194
Hin6I	GCGC	3 cut(s)	323, 399, 628
HinP1I	GCGC	3 cut(s)	323, 399, 628
HincII	GTYRAC	3 cut(s)	853, 1463, 1801
HindII	GTYRAC	3 cut(s)	853, 1463, 1801
HindIII	AAGCTT	4 cut(s)	474, 622, 1630, 2097
HinfI	GANTC	7 cut(s)	43, 114, 180, 222, 417, 562, 603
HpaII	CCGG	4 cut(s)	49, 75, 136, 1653
HphI	GGTGA	7 cut(s)	64, 109, 125, 294, 511, 760, 1313
Hpy188I	TCNGA	7 cut(s)	113, 199, 602, 1264, 1413, 1486, 1737
Hpy188III	TCNNGA	5 cut(s)	1505, 1580, 1664, 1976, 2014
Hpy99I	CGWCG	3 cut(s)	23, 199, 254
HpyAV	CCTTC	7 cut(s)	8, 133, 739, 1055, 1102, 1337, 1856
HpyCH4III	ACNGT	3 cut(s)	464, 1308, 1884
HpyCH4IV	ACGT	4 cut(s)	284, 1361, 1408, 1797
HpyF10VI	GCNNNNNNNGC	5 cut(s)	92, 625, 932, 1013, 1629
HpySE526I	ACGT	4 cut(s)	284, 1361, 1408, 1797
Hsp92I	GRCGYC	2 cut(s)	21, 194
HspAI	GCGC	3 cut(s)	323, 399, 628
Ksp22I	TGATCA	1 cut(s)	940
Kzo9I	GATC	9 cut(s)	157, 871, 940, 1087, 1403, 1576, 1836, 2039, 2114
LguI	GCTCTTC	1 cut(s)	1380
LmnI	GCTCC	6 cut(s)	51, 77, 173, 217, 1024, 1148
Lsp1109I	GCAGC	3 cut(s)	337, 453, 604
LweI	GCATC	6 cut(s)	412, 579, 1234, 1831, 1900, 1973
MaeI	CTAG	3 cut(s)	375, 807, 1610
MaeII	ACGT	4 cut(s)	284, 1361, 1408, 1797
MaeIII	GTNAC	6 cut(s)	70, 181, 280, 367, 748, 1919
MalI	GATC	9 cut(s)	159, 873, 942, 1089, 1405, 1578, 1838, 2041, 2116
MbiI	CCGCTC	2 cut(s)	86, 412
MboI	GATC	9 cut(s)	157, 871, 940, 1087, 1403, 1576, 1836, 2039, 2114
MflI	RGATCY	4 cut(s)	157, 871, 1087, 2114
MhlI	GDGCHC	1 cut(s)	1153
MlsI	TGGCCA	2 cut(s)	1007, 1515
MluNI	TGGCCA	2 cut(s)	1007, 1515
MlyI	GAGTC	3 cut(s)	189, 231, 612
MmeI	TCCRAC	4 cut(s)	177, 778, 1549, 1856
Mox20I	TGGCCA	2 cut(s)	1007, 1515
MscI	TGGCCA	2 cut(s)	1007, 1515
MseI	TTAA	7 cut(s)	572, 711, 1197, 1223, 1707, 1818, 2063
MslI	CAYNNNNRTG	3 cut(s)	612, 1263, 1362
Msp20I	TGGCCA	2 cut(s)	1007, 1515
MspA1I	CMGCKG	1 cut(s)	1458
MspCI	CTTAAG	1 cut(s)	710
MspI	CCGG	4 cut(s)	49, 75, 136, 1653
MspR9I	CCNGG	1 cut(s)	2034
Mva1269I	GAATGC	2 cut(s)	508, 1258
MvaI	CCWGG	1 cut(s)	2034
MwoI	GCNNNNNNNGC	5 cut(s)	92, 625, 932, 1013, 1629
NdeII	GATC	9 cut(s)	157, 871, 940, 1087, 1403, 1576, 1836, 2039, 2114
NlaIV	GGNNCC	7 cut(s)	53, 79, 169, 774, 1596, 2031, 2049
NmeAIII	GCCGAG	3 cut(s)	427, 888, 1273
NmuCI	GTSAC	3 cut(s)	70, 181, 748
NspI	RCATGY	3 cut(s)	431, 448, 2008
NspV	TTCGAA	1 cut(s)	388
PaeI	GCATGC	2 cut(s)	448, 2008
PagI	TCATGA	1 cut(s)	1975
PaqCI	CACCTGC	1 cut(s)	970
PasI	CCCWGGG	1 cut(s)	2033
PciI	ACATGT	1 cut(s)	427
PciSI	GCTCTTC	1 cut(s)	1380
PcsI	WCGNNNNNNNCGW	1 cut(s)	99
PctI	GAATGC	2 cut(s)	508, 1258
PfeI	GAWTC	4 cut(s)	43, 114, 417, 562
PflFI	GACNNNGTC	1 cut(s)	23
PkrI	GCNGC	3 cut(s)	327, 443, 594
PleI	GAGTC	3 cut(s)	188, 230, 611
PpsI	GAGTC	3 cut(s)	188, 230, 611
Ppu21I	YACGTR	2 cut(s)	285, 1362
PscI	ACATGT	1 cut(s)	427
Psp124BI	GAGCTC	1 cut(s)	1153
Psp1406I	AACGTT	1 cut(s)	1797
Psp6I	CCWGG	1 cut(s)	2032
PspEI	GGTNACC	1 cut(s)	748
PspGI	CCWGG	1 cut(s)	2032
PspN4I	GGNNCC	7 cut(s)	53, 79, 169, 774, 1596, 2031, 2049
PspPI	GGNCC	4 cut(s)	244, 1312, 1522, 2029
PsuI	RGATCY	4 cut(s)	157, 871, 1087, 2114
PsyI	GACNNNGTC	1 cut(s)	23
RsaI	GTAC	3 cut(s)	461, 906, 1334
RsaNI	GTAC	3 cut(s)	460, 905, 1333
RseI	CAYNNNNRTG	3 cut(s)	612, 1263, 1362
SacI	GAGCTC	1 cut(s)	1153
SapI	GCTCTTC	1 cut(s)	1380
SaqAI	TTAA	7 cut(s)	572, 711, 1197, 1223, 1707, 1818, 2063
SatI	GCNGC	3 cut(s)	326, 442, 593
Sau3AI	GATC	9 cut(s)	157, 871, 940, 1087, 1403, 1576, 1836, 2039, 2114
Sau96I	GGNCC	4 cut(s)	244, 1312, 1522, 2029
SchI	GAGTC	3 cut(s)	189, 231, 612
ScrFI	CCNGG	1 cut(s)	2034
SduI	GDGCHC	1 cut(s)	1153
SfaNI	GCATC	6 cut(s)	412, 579, 1234, 1831, 1900, 1973
SfuI	TTCGAA	1 cut(s)	388
SinI	GGWCC	2 cut(s)	244, 1522
SmiMI	CAYNNNNRTG	3 cut(s)	612, 1263, 1362
SmlI	CTYRAG	4 cut(s)	525, 710, 1154, 2094
SmoI	CTYRAG	4 cut(s)	525, 710, 1154, 2094
SnaBI	TACGTA	1 cut(s)	285
SpeI	ACTAGT	1 cut(s)	374
SphI	GCATGC	2 cut(s)	448, 2008
SsiI	CCGC	9 cut(s)	58, 84, 120, 215, 269, 412, 1272, 1458, 1952
SspMI	CTAG	3 cut(s)	375, 807, 1610
SstI	GAGCTC	1 cut(s)	1153
StyD4I	CCNGG	1 cut(s)	2032
StyI	CCWWGG	1 cut(s)	171
TaaI	ACNGT	3 cut(s)	464, 1308, 1884
TaiI	ACGT	4 cut(s)	287, 1364, 1411, 1800
TaqI	TCGA	8 cut(s)	178, 236, 388, 420, 1287, 1477, 1770, 2106
TatI	WGTACW	1 cut(s)	1332
TfiI	GAWTC	4 cut(s)	43, 114, 417, 562
Tru1I	TTAA	7 cut(s)	572, 711, 1197, 1223, 1707, 1818, 2063
Tru9I	TTAA	7 cut(s)	572, 711, 1197, 1223, 1707, 1818, 2063
TscAI	CASTG	3 cut(s)	679, 828, 1954
TseFI	GTSAC	3 cut(s)	70, 181, 748
TseI	GCWGC	3 cut(s)	325, 441, 592
Tsp45I	GTSAC	3 cut(s)	70, 181, 748
TspDTI	ATGAA	9 cut(s)	305, 663, 1009, 1536, 1641, 1704, 1757, 1817, 1964
TspGWI	ACGGA	1 cut(s)	20
TspRI	CASTG	3 cut(s)	679, 828, 1954
Tth111I	GACNNNGTC	1 cut(s)	23
Vha464I	CTTAAG	1 cut(s)	710
VpaK11BI	GGWCC	2 cut(s)	244, 1522
XapI	RAATTY	2 cut(s)	1174, 1752
XceI	RCATGY	3 cut(s)	431, 448, 2008
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1214
XmiI	GTMKAC	1 cut(s)	694
XspI	CTAG	3 cut(s)	375, 807, 1610

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.