FvH4_5g00080

Aminoacyl-tRNA editing domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002840
Physical Location & Seq
Reverse (-)
50800 .. 56717
5918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00080.t6

Sequence Viewer

Length: 687 bp
ATGGGGGACTCACTAGCAGAGCTGGAGCGAATCCAAACCCAAATCCTCGACCGCACTTCAGACCTGGAGATCTCCTTCGCCTCTCTTCTTCCCCAAACCGCCACTCAAAACGACGCCGTCTACGGCGGCACCGGAGCTCGCCTCTCCGCGATTCTCAAATCCAAAGGCGTCAACGACTTCTCTTTTAAGAGAGTCGCCTCCGATTACTACGACTGGCCTCTCGAATCCCGGCGCGACGCCGTCGGAGCCGCCTCCGTCCAACATCTCTGCAAAAGCATTGTTCTGGTTAACACTCAAGCCATTCCTAGTGTGGTTGACTGTAGTGACCGCAACAATTCAAAGTATTACGTTGTGGTCGTTCAGTATGCTGCTAGGTTTAATGCCGAAGCTGTTAAAAACTTCTTGTATACGCTCAACCAAGGCAAAATCGCCAAAAAGAAGTTCAACTTGAGGCTTGCACCTGAGGAGACTTCAATACAATTGACTGGGTTTGAGCACAACGCAGTTACATGTATTGGCATGAAAACAGAAATTCCGGTTATTTTGGATGAAGCAATTCAGAAACTTAATCCTGATTTCTTCTGGTTGGGTGGTGGAGAGGTTGATCTGAAGCTGGGTATCAAAACCTCCCAGTTCATAAATGTTGTTAAACCTTTTATTGTGAGCTGTAGTAGTGCACTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_edit

Protein Analysis

229

Amino Acids

25.12

Weight (kDa)

6.16

Isoelectric Point (pI)

23.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 83 - 212 4.9e-09 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 128
AccI GTMKAC 2 cut(s) 120, 407
AccII CGCG 2 cut(s) 149, 234
AciI CCGC 6 cut(s) 52, 99, 126, 147, 249, 328
AcsI RAATTY 1 cut(s) 531
AcuI CTGAAG 2 cut(s) 42, 629
AcyI GRCGYC 3 cut(s) 114, 168, 237
AflIII ACRYGT 1 cut(s) 509
AgsI TTSAA 3 cut(s) 339, 445, 474
AjnI CCWGG 1 cut(s) 63
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AluBI AGCT 5 cut(s) 22, 137, 389, 613, 666
AluI AGCT 5 cut(s) 22, 137, 389, 613, 666
Alw21I GWGCWC 3 cut(s) 139, 498, 679
Alw26I GTCTC 1 cut(s) 461
Alw44I GTGCAC 1 cut(s) 675
AoxI GGCC 1 cut(s) 215
ApaLI GTGCAC 1 cut(s) 675
ApeKI GCWGC 1 cut(s) 368
ApoI RAATTY 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 1 cut(s) 234
AsuC2I CCSGG 1 cut(s) 229
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 679
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 139
Bbv12I GWGCWC 3 cut(s) 139, 498, 679
BbvI GCAGC 1 cut(s) 355
BceAI ACGGC 3 cut(s) 101, 139, 224
BciT130I CCWGG 1 cut(s) 65
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 3 cut(s) 14, 306, 372
BfmI CTRYAG 2 cut(s) 319, 667
BglII AGATCT 1 cut(s) 69
BisI GCNGC 3 cut(s) 127, 249, 369
BlsI GCNGC 3 cut(s) 128, 250, 370
Bme1390I CCNGG 2 cut(s) 65, 229
BmiI GGNNCC 2 cut(s) 130, 247
BmrFI CCNGG 2 cut(s) 65, 229
BmrI ACTGGG 2 cut(s) 495, 625
BmuI ACTGGG 2 cut(s) 495, 625
BplI GAGNNNNNCTC 2 cut(s) 126, 158
BpmI CTGGAG 2 cut(s) 44, 86
BpuEI CTTGAG 2 cut(s) 279, 469
BpuMI CCSGG 1 cut(s) 229
BsaHI GRCGYC 3 cut(s) 114, 168, 237
BsaJI CCNNGG 1 cut(s) 418
BsaWI WCCGGW 2 cut(s) 131, 535
Bse1I ACTGG 3 cut(s) 218, 490, 631
Bse21I CCTNAGG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 1 cut(s) 553
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 3 cut(s) 218, 490, 631
BseRI GAGGAG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 1 cut(s) 355
BseYI CCCAGC 1 cut(s) 613
Bsh1236I CGCG 2 cut(s) 149, 234
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 1 cut(s) 217
BshNI GGYRCC 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 3 cut(s) 139, 498, 679
BsiSI CCGG 3 cut(s) 132, 229, 536
BslFI GGGAC 1 cut(s) 20
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 1 cut(s) 217
Bsp1286I GDGCHC 3 cut(s) 139, 498, 679
Bsp143I GATC 2 cut(s) 69, 604
BspACI CCGC 6 cut(s) 52, 99, 126, 147, 249, 328
BspANI GGCC 1 cut(s) 217
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 2 cut(s) 149, 234
BspLI GGNNCC 2 cut(s) 130, 247
BspT107I GGYRCC 1 cut(s) 128
BsrI ACTGG 3 cut(s) 218, 490, 631
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 2 cut(s) 69, 604
BssNAI GTATAC 1 cut(s) 408
BssNI GRCGYC 3 cut(s) 114, 168, 237
BssT1I CCWWGG 1 cut(s) 418
Bst1107I GTATAC 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 1 cut(s) 90
BstACI GRCGYC 3 cut(s) 114, 168, 237
BstC8I GCNNGC 2 cut(s) 139, 456
BstDEI CTNAG 1 cut(s) 462
BstF5I GGATG 1 cut(s) 553
BstFNI CGCG 2 cut(s) 149, 234
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 2 cut(s) 72, 607
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 2 cut(s) 69, 604
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 65
BstNSI RCATGY 1 cut(s) 513
BstSCI CCNGG 2 cut(s) 63, 227
BstSFI CTRYAG 2 cut(s) 319, 667
BstSLI GKGCMC 1 cut(s) 679
BstUI CGCG 2 cut(s) 149, 234
BstV1I GCAGC 1 cut(s) 355
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BstZ17I GTATAC 1 cut(s) 408
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 1 cut(s) 217
BtsCI GGATG 1 cut(s) 553
Cac8I GCNNGC 2 cut(s) 139, 456
CfoI GCGC 1 cut(s) 234
CseI GACGC 3 cut(s) 122, 157, 245
CviAII CATG 2 cut(s) 510, 520
CviJI RGCY 9 cut(s) 22, 137, 217, 248, 299, 389, 454, 613, 666
CviKI_1 RGCY 9 cut(s) 22, 137, 217, 248, 299, 389, 454, 613, 666
DdeI CTNAG 1 cut(s) 462
DpnI GATC 2 cut(s) 71, 606
DpnII GATC 2 cut(s) 69, 604
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 139
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 2 cut(s) 42, 629
Eco81I CCTNAGG 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 2 cut(s) 513, 523
FaiI YATR 5 cut(s) 366, 408, 511, 521, 638
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 1 cut(s) 20
FatI CATG 2 cut(s) 509, 519
FblI GTMKAC 2 cut(s) 120, 407
Fnu4HI GCNGC 3 cut(s) 127, 249, 369
FokI GGATG 1 cut(s) 560
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 3 cut(s) 127, 249, 369
FspBI CTAG 3 cut(s) 14, 306, 372
GlaI GCGC 1 cut(s) 233
GluI GCNGC 3 cut(s) 127, 249, 369
GsaI CCCAGC 1 cut(s) 617
GsuI CTGGAG 2 cut(s) 44, 86
HaeIII GGCC 1 cut(s) 217
HapII CCGG 3 cut(s) 132, 229, 536
HgaI GACGC 3 cut(s) 122, 157, 245
HhaI GCGC 1 cut(s) 234
Hin1I GRCGYC 3 cut(s) 114, 168, 237
Hin1II CATG 2 cut(s) 513, 523
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HincII GTYRAC 3 cut(s) 172, 289, 316
HindII GTYRAC 3 cut(s) 172, 289, 316
HinfI GANTC 5 cut(s) 8, 30, 151, 192, 224
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 3 cut(s) 132, 229, 536
Hpy166II GTNNAC 6 cut(s) 121, 172, 289, 316, 408, 677
Hpy188I TCNGA 5 cut(s) 61, 202, 245, 561, 609
Hpy188III TCNNGA 2 cut(s) 221, 572
Hpy8I GTNNAC 6 cut(s) 121, 172, 289, 316, 408, 677
Hpy99I CGWCG 3 cut(s) 116, 239, 245
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 3 cut(s) 270, 458, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 3 cut(s) 114, 168, 237
Hsp92II CATG 2 cut(s) 513, 523
HspAI GCGC 1 cut(s) 232
KspAI GTTAAC 1 cut(s) 289
Kzo9I GATC 2 cut(s) 69, 604
LmnI GCTCC 3 cut(s) 25, 134, 245
Lsp1109I GCAGC 1 cut(s) 355
MaeI CTAG 3 cut(s) 14, 306, 372
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 323, 505
MalI GATC 2 cut(s) 71, 606
MboI GATC 2 cut(s) 69, 604
MboII GAAGA 3 cut(s) 77, 80, 571
MfeI CAATTG 1 cut(s) 479
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 3 cut(s) 139, 498, 679
MluCI AATT 4 cut(s) 334, 479, 531, 555
MlyI GAGTC 2 cut(s) 2, 201
MmeI TCCRAC 2 cut(s) 223, 283
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 6 cut(s) 186, 288, 378, 393, 567, 648
MspI CCGG 3 cut(s) 132, 229, 536
MspR9I CCNGG 2 cut(s) 65, 229
MunI CAATTG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 2 cut(s) 149, 234
MwoI GCNNNNNNNGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 229
NdeII GATC 2 cut(s) 69, 604
NlaIII CATG 2 cut(s) 513, 523
NlaIV GGNNCC 2 cut(s) 130, 247
NmuCI GTSAC 1 cut(s) 323
NspI RCATGY 1 cut(s) 513
PciI ACATGT 1 cut(s) 509
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 3 cut(s) 30, 151, 224
PflFI GACNNNGTC 2 cut(s) 116, 239
PkrI GCNGC 3 cut(s) 128, 250, 370
PleI GAGTC 2 cut(s) 2, 200
PpsI GAGTC 2 cut(s) 2, 200
PscI ACATGT 1 cut(s) 509
Psp124BI GAGCTC 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 130, 247
PsuI RGATCY 1 cut(s) 69
PsyI GACNNNGTC 2 cut(s) 116, 239
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 6 cut(s) 186, 288, 378, 393, 567, 648
SatI GCNGC 3 cut(s) 127, 249, 369
Sau3AI GATC 2 cut(s) 69, 604
SchI GAGTC 2 cut(s) 2, 201
ScrFI CCNGG 2 cut(s) 65, 229
SduI GDGCHC 3 cut(s) 139, 498, 679
SfcI CTRYAG 2 cut(s) 319, 667
SmlI CTYRAG 2 cut(s) 294, 448
SmoI CTYRAG 2 cut(s) 294, 448
Sse9I AATT 4 cut(s) 334, 479, 531, 555
SsiI CCGC 6 cut(s) 52, 99, 126, 147, 249, 328
SspMI CTAG 3 cut(s) 14, 306, 372
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 2 cut(s) 63, 227
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 1 cut(s) 351
TaqI TCGA 2 cut(s) 48, 222
TasI AATT 4 cut(s) 334, 479, 531, 555
TauI GCSGC 2 cut(s) 129, 251
TfiI GAWTC 3 cut(s) 30, 151, 224
Tru1I TTAA 6 cut(s) 186, 288, 378, 393, 567, 648
Tru9I TTAA 6 cut(s) 186, 288, 378, 393, 567, 648
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 368
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 3 cut(s) 536, 564, 625
TspGWI ACGGA 1 cut(s) 244
Tth111I GACNNNGTC 2 cut(s) 116, 239
VneI GTGCAC 1 cut(s) 675
XapI RAATTY 1 cut(s) 531
XceI RCATGY 1 cut(s) 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmiI GTMKAC 2 cut(s) 120, 407
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 3 cut(s) 14, 306, 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.