MD00G1115100.v1.1

Aminoacyl-tRNA editing domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002840
Physical Location & Seq
Reverse (-)
24479873 .. 24483545
3673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1115100.v1.1.491

Sequence Viewer

Length: 660 bp
ATGGAAGACTCACTAGCAAAACTAGAGCGAATCCAAACCCAAATCATCGAACGCATATCAAAGCTTGAGCTCTCCACCTCCGCTTCTCTTCAGCAATTCACCGCTACCCCCATCCCTACCTCAACTCAAAACGCCGCCGTCAGCGCCAACGAAGCCCGCCTCTCCGCCATTCTCCTAGCCAATGACGTAAAAAACTTCGCCTATAAGAGGGTTCCCTCCGATTACTACGATTGGTCTCTCGACGCCCGGCGCGACGCTCTCGGCGCTGCCTCAATCCACCATCTCTGCAAAAGCATTATCTTGTACACTGCTCGGTTTAATGCTGAAGCTGTTAAGAACTTCTTGTATTCGCTCAACGAGGGCAAGATAGCGAAAAAGAAATTCAACTGTCAGTTCTTGGTGATAGTGGTTGTTATTGGGTATGCATTGAGACTTGCACCTGAGGATAAATCAATAGAATTGACTGGATTTGAGCACAATGCAGTTACATGTGTTGGCATGAAAACAGACATTCCGGTGATTTTGGATGAAGCAATTGAGAAACTTAATCCCGATTTCTTCTGGTTGGGCGGCGGCGAGGTTGATCTGAAGCTGGGCATCAGAACCTCAGAATTTATAAACTTTGCTAAACCTTTTATTGTGAGCTGCAGTAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_edit

Protein Analysis

220

Amino Acids

24.28

Weight (kDa)

6.32

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 142 - 207 2.6e-06 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 617
AccII CGCG 1 cut(s) 252
AciI CCGC 7 cut(s) 81, 102, 135, 157, 165, 570, 573
AcsI RAATTY 2 cut(s) 380, 611
AcuI CTGAAG 3 cut(s) 74, 345, 608
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 207
AflIII ACRYGT 1 cut(s) 488
AgsI TTSAA 1 cut(s) 385
AluBI AGCT 5 cut(s) 64, 70, 329, 592, 645
AluI AGCT 5 cut(s) 64, 70, 329, 592, 645
Alw21I GWGCWC 2 cut(s) 72, 477
Alw26I GTCTC 2 cut(s) 240, 424
ApeKI GCWGC 2 cut(s) 266, 645
ApoI RAATTY 2 cut(s) 380, 611
AspLEI GCGC 3 cut(s) 146, 252, 266
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 91, 412, 529
AxyI CCTNAGG 1 cut(s) 441
BanII GRGCYC 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 72, 477
BbvI GCAGC 2 cut(s) 253, 632
BccI CCATC 2 cut(s) 119, 288
BceAI ACGGC 1 cut(s) 122
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 2 cut(s) 240, 424
BfaI CTAG 3 cut(s) 14, 23, 176
BfmI CTRYAG 1 cut(s) 646
BfoI RGCGCY 2 cut(s) 147, 267
BisI GCNGC 5 cut(s) 135, 267, 571, 574, 646
BlsI GCNGC 5 cut(s) 136, 268, 572, 575, 647
Bme1390I CCNGG 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 1 cut(s) 247
BmsI GCATC 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 240
BsaWI WCCGGW 1 cut(s) 514
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 469
Bse21I CCTNAGG 1 cut(s) 441
BseGI GGATG 2 cut(s) 111, 532
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 2 cut(s) 432, 621
BseNI ACTGG 1 cut(s) 469
BseXI GCAGC 2 cut(s) 253, 632
BseYI CCCAGC 1 cut(s) 592
Bsh1236I CGCG 1 cut(s) 252
BsiHKAI GWGCWC 2 cut(s) 72, 477
BsiSI CCGG 2 cut(s) 247, 515
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 2 cut(s) 240, 424
Bso31I GGTCTC 1 cut(s) 240
Bsp1286I GDGCHC 2 cut(s) 72, 477
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 1 cut(s) 583
BspACI CCGC 7 cut(s) 81, 102, 135, 157, 165, 570, 573
BspCNI CTCAG 2 cut(s) 433, 620
BspFNI CGCG 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 213
BspMAI CTGCAG 1 cut(s) 650
BspTNI GGTCTC 1 cut(s) 240
BsrGI TGTACA 1 cut(s) 303
BsrI ACTGG 1 cut(s) 469
BssMI GATC 1 cut(s) 583
BssNI GRCGYC 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 389
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 243
BstAUI TGTACA 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 2 cut(s) 441, 607
BstENI CCTNNNNNAGG 1 cut(s) 205
BstF5I GGATG 2 cut(s) 111, 532
BstFNI CGCG 1 cut(s) 252
BstH2I RGCGCY 2 cut(s) 147, 267
BstHHI GCGC 3 cut(s) 146, 252, 266
BstKTI GATC 1 cut(s) 586
BstMAI GTCTC 2 cut(s) 240, 424
BstMBI GATC 1 cut(s) 583
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 263
BstNSI RCATGY 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 1 cut(s) 646
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 2 cut(s) 253, 632
BstV2I GAAGAC 1 cut(s) 12
Bsu36I CCTNAGG 1 cut(s) 441
BtsCI GGATG 2 cut(s) 111, 532
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 1 cut(s) 157
CfoI GCGC 3 cut(s) 146, 252, 266
CseI GACGC 2 cut(s) 251, 263
Csp6I GTAC 1 cut(s) 304
CviAII CATG 2 cut(s) 489, 499
CviJI RGCY 7 cut(s) 64, 70, 155, 179, 329, 592, 645
CviKI_1 RGCY 7 cut(s) 64, 70, 155, 179, 329, 592, 645
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 2 cut(s) 441, 607
DpnI GATC 1 cut(s) 585
DpnII GATC 1 cut(s) 583
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 154
Ecl136II GAGCTC 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 3 cut(s) 74, 345, 608
Eco81I CCTNAGG 1 cut(s) 441
EcoICRI GAGCTC 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 72
FaeI CATG 2 cut(s) 492, 502
FaiI YATR 6 cut(s) 56, 204, 423, 490, 500, 617
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FatI CATG 2 cut(s) 488, 498
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 5 cut(s) 135, 267, 571, 574, 646
FokI GGATG 2 cut(s) 98, 539
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 5 cut(s) 135, 267, 571, 574, 646
FspBI CTAG 3 cut(s) 14, 23, 176
GlaI GCGC 3 cut(s) 145, 251, 265
GluI GCNGC 5 cut(s) 135, 267, 571, 574, 646
GsaI CCCAGC 1 cut(s) 596
HaeII RGCGCY 2 cut(s) 147, 267
HapII CCGG 2 cut(s) 247, 515
HgaI GACGC 2 cut(s) 251, 263
HhaI GCGC 3 cut(s) 146, 252, 266
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 2 cut(s) 492, 502
Hin6I GCGC 3 cut(s) 144, 250, 264
HinP1I GCGC 3 cut(s) 144, 250, 264
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 2 cut(s) 8, 30
HpaII CCGG 2 cut(s) 247, 515
HphI GGTGA 3 cut(s) 91, 412, 529
Hpy166II GTNNAC 1 cut(s) 306
Hpy188I TCNGA 4 cut(s) 220, 588, 602, 610
Hpy188III TCNNGA 3 cut(s) 239, 551, 657
Hpy8I GTNNAC 1 cut(s) 306
Hpy99I CGWCG 2 cut(s) 245, 257
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 5 cut(s) 288, 425, 437, 482, 648
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 263
HpyF3I CTNAG 2 cut(s) 441, 607
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 2 cut(s) 492, 502
HspAI GCGC 3 cut(s) 144, 250, 264
Kzo9I GATC 1 cut(s) 583
LpnPI CCDG 6 cut(s) 260, 450, 453, 528, 547, 578
Lsp1109I GCAGC 2 cut(s) 253, 632
LweI GCATC 1 cut(s) 606
MaeI CTAG 3 cut(s) 14, 23, 176
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 484
MalI GATC 1 cut(s) 585
MboI GATC 1 cut(s) 583
MboII GAAGA 3 cut(s) 17, 80, 550
MfeI CAATTG 1 cut(s) 534
MhlI GDGCHC 2 cut(s) 72, 477
MluCI AATT 5 cut(s) 95, 380, 458, 534, 611
MlyI GAGTC 1 cut(s) 2
Mph1103I ATGCAT 1 cut(s) 427
MseI TTAA 3 cut(s) 318, 333, 546
MslI CAYNNNNRTG 1 cut(s) 515
MspI CCGG 2 cut(s) 247, 515
MspR9I CCNGG 1 cut(s) 247
MunI CAATTG 1 cut(s) 534
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 263
NciI CCSGG 1 cut(s) 247
NdeII GATC 1 cut(s) 583
NlaIII CATG 2 cut(s) 492, 502
NlaIV GGNNCC 1 cut(s) 213
NmeAIII GCCGAG 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 427
NspI RCATGY 1 cut(s) 492
PciI ACATGT 1 cut(s) 488
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 1 cut(s) 30
PkrI GCNGC 5 cut(s) 136, 268, 572, 575, 647
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PscI ACATGT 1 cut(s) 488
PsiI TTATAA 1 cut(s) 617
Psp124BI GAGCTC 1 cut(s) 72
PspFI CCCAGC 1 cut(s) 592
PspN4I GGNNCC 1 cut(s) 213
PstI CTGCAG 1 cut(s) 650
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 1 cut(s) 515
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 318, 333, 546
SatI GCNGC 5 cut(s) 135, 267, 571, 574, 646
Sau3AI GATC 1 cut(s) 583
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 2 cut(s) 72, 477
SfaNI GCATC 1 cut(s) 606
SfcI CTRYAG 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 515
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 5 cut(s) 95, 380, 458, 534, 611
SsiI CCGC 7 cut(s) 81, 102, 135, 157, 165, 570, 573
SspMI CTAG 3 cut(s) 14, 23, 176
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 245
TaaI ACNGT 1 cut(s) 389
TaiI ACGT 1 cut(s) 189
TaqI TCGA 2 cut(s) 48, 240
TasI AATT 5 cut(s) 95, 380, 458, 534, 611
TatI WGTACW 1 cut(s) 303
TauI GCSGC 3 cut(s) 137, 573, 576
TfiI GAWTC 1 cut(s) 30
Tru1I TTAA 3 cut(s) 318, 333, 546
Tru9I TTAA 3 cut(s) 318, 333, 546
TscAI CASTG 1 cut(s) 313
TseI GCWGC 2 cut(s) 266, 645
TspDTI ATGAA 2 cut(s) 515, 543
TspRI CASTG 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 205
XapI RAATTY 2 cut(s) 380, 611
XceI RCATGY 1 cut(s) 492
XspI CTAG 3 cut(s) 14, 23, 176
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.