FvH4_5g01860

Methyl-CpG binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002094
Physical Location & Seq
Reverse (-)
1161375 .. 1168547
7173 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01860.t3

Sequence Viewer

Length: 2970 bp
ATGGTGGCCGCGAGCTCGCCGGACTGGCTCCCCGCCGGCTGGAGCGTCCAATTTAGAGCCCAGAACCGTGGTCGCCGAAGTGTGATTTACATCGAGTTGGAAACCGGGAAGAAGTTCTTCTCCAAGGACGATGTTATACGGTATACTAAAAAGACCACCAACTGTGGTGAAGAGCCCAAGCCTACAAATACCAAAAACCCCTCCAGTAAAAGTCATTTGCAGGTGGTAGCAAACACAGTTGAATATCCTGAATGGTTGCCAGATGGCTGGAAAGTCGAATTGAGAACTCGAAAATCTGGTGTGCAAGTTGGAAAGGAGTACAAGTGTTACATTGATCCGTCAAGGGAATGTACATTTTATTCTAAGCCAGAGGTATTTCGACACCTGAGAACCGTAAAGCGCAAGAGCTACAAATCCAGAAGTCACAAAACGGTAAACCGCAAAAGATGCATTATCAAAACCAAGACCGTCACTGCCATGCACTTCCCAAGCAAAGTTGTGGTTGAGAAGCATAATGTGGAAGATTTACCGCCAGGGTGGATATTGGAGATCAAAGTTAGAAAGGTTGCAAATCGAATAAGAAGAGATCCGTATTACACTGATCCAGTGCATGGATATATCTTCCGTTCCAAAAAGGATGTTATCCGCTACCTACAAACTGGAGAGACCAGTAAACATACAATCAAACCAAAGAGCATGTGCACCAATGATCTAAAGTTGACTGATGATGAAATTGCACTGTCATCTGCAACTAAAAACATATCTGCTATGGCTCCAATTACTGAGGTGTTCCTGGAGAAGCATTTCATTGAAAATCTGGTTGAAGATTGTGTAGAAACTAAGGGAGAATGTTCCCCAAGCTGGTCATCACACCCAAAAGCTGAACCCTCTAGCAAACATGTGGGTAAAAGGTCATCACAACGACTAGCTGCACTCTCTAACAAACGTGAGGCTAAAAAGTCCAAGTACCAAGACCAGAGGGTAAATGTTGCTTTGGCTCCGACAGCCGAGGTTGTCCCACATAAGCATTTAAGTGAGAGTGTGGTTGTAAATTGTGCCAAAACCAAGGAGGATTGTTCTACAAGCAAGTTATTGCAGCCAAAAGCAGAGATCTCCAGCAAGTGTGAAGAAAAAACTGCATTGGTTGGTGGTTCCTCAACAGATCCTGGCAAATTGAATTTTTCTGAAGATGACCCTGCTTTGACTCCCACTGCTAACACCTTAAATACGGACTCACATATGAGTTGGATGGAAAAGAGCAACTCCAGGAGAATCCAAACAGATGCAAGAAATTCAAAAAAGAAGGAAAACCTTGATTTGCCTCGTCGGTCCTCAAAACGACTTGCTGGGTTTGAACCAGAGTCGGTAAATAGCCTGGTTTCCACTGTAAAGGCTCTTCAATTTGCAACTAGGAAGCCTAGTAAAGGTGGTCAGAGTCCAGATGCAGGTTTGGCTTCAGATGATTTTGTAAATAGAGCATCTCAGCAGATTGGACATGCATCGGAAACAGAAGTTGTACATCATATTAGAACTGAAATAGTATCTACAGTACAAGGAGAGTCATTGAGTGAGGTCAAGAGGCCATTTGATAGTCTTGAACTACTGCCTGTAGATACTTTGGTTTCCAATGATCAAACTCTTCACCTTGCAACTGGAAAGTCAAGTAGAGCTGATGCCAACCAAGAAATGGGTTTCACTTCAGATGATAATATCAATGGAGTATCTCTGCAACTTGGGGCTGCATTAGAACCAGAGGCTTTACATGATATGTTAACTGACATAAACTCTACTGCACATGGAGAGTCATCAAACAAGGGAAAGACGTTTCTTGGTGACAAAGCAATTCCTGAAGAGCAACATCATAGACTGGAAGCTGAGAACATGTTAGGTGAGAAGCCCGGACCAGAGCTCTCCTTACTGTTCGGGTCCGACCCATGCTTAGAATTCGCCTTCAAAACCCTTACTGGGGAATTGCCGATAGCTGATGGTGTGGATAAAGAACCTTTTCTGAAAGCTGCAGCTGATACTGTGCAAAAAGAGAACTCACTTGAAAATGGGAGCTGCAGTAGGAAAACAAGGCAAAGTTCAAACAAATCTAAGAAAACTAAAGAACTAAAGTTGCCTCGTCGGACTTCGAAACGGCTGGCTGGGGTTGAACCTGAGCTGCTAGCCAAACCATTGTCCAGTGAAACAGTTCTTCGAAATGCAACAGCAAAGTCTTGTAGAAGTAAACCGCTCCAAGCTGTAGATTCTGCACATGAAGCACCTCAACAGTTTGAGGCTGGTCAAGAGAACAAGGTTGCTCATGATTCGTGTACTACTGTAAGTCCTTCAATTGATGAGGAACCATTGAACAAGATTGAGAGATCTCTCAAGGACCAAGCTGTTCCTGAGGAGAAACCCCGGCAGTTGGAAATTGAGAAGGCAGCTGTCGAGAATCCAGTGTCACAGTTTTCATTCCCTTTTCTGGATACTTGGTCTGATCCATGCCTTGACTTTGCATTTAAGACCCTTACCGGTGAAATTCCGATAGATGATGATTTGTTTCAGGGTTACTTTGAGGAAAACCTTGTCACCTCTCACAACCAGAGAGATAGCATGGCACTGCCAGATTTTGGCTCACCTAACTTTTTCCCAAGTGACATATCCCCTCAGTTCAATGCACCAGAACAATCTACCTCGGGCCAACTATTTCCCATGAATACTACTTTGCCACCTGCCGGAAATGTGAGTGTGTCAGATTGCAATGGGGTGGGATTCGGCCAACAGTTATCCATGAGTTCTTCATTTCTGCCTGCGGGAAATTTGAGAATTTCAGGTGGCAGTGGTGTTGGTTCCTGTCAACATTTATCCGTGAATACTTCATTCTCGCCCTCAGGAAATGTAGGCCTACCAAATTGGAGTGGGGTTGGTGCACAGAACCCTTGCTTAGGATGCAACAAGGAGTTTCATGGAAAGGTGAAGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBD

Protein Analysis

990

Amino Acids

109.05

Weight (kDa)

8.31

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 176 - 223 9e-06 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 211, 2725
Acc36I ACCTGC 3 cut(s) 211, 1436, 2725
AccB7I CCANNNNNTGG 3 cut(s) 611, 1687, 2615
AccBSI CCGCTC 1 cut(s) 2236
AccI GTMKAC 1 cut(s) 143
AccII CGCG 1 cut(s) 11
AciI CCGC 7 cut(s) 9, 33, 439, 530, 646, 2234, 2798
AclWI GGATC 5 cut(s) 329, 581, 596, 1157, 2477
AcoI YGGCCR 2 cut(s) 6, 2761
AcsI RAATTY 6 cut(s) 1177, 1291, 1943, 2523, 2803, 2811
AcuI CTGAAG 4 cut(s) 1206, 1440, 1683, 1869
AfaI GTAC 6 cut(s) 320, 352, 968, 1518, 1551, 2317
AflIII ACRYGT 2 cut(s) 898, 1881
AgeI ACCGGT 1 cut(s) 2516
AjnI CCWGG 5 cut(s) 532, 792, 1165, 1265, 1374
Alw21I GWGCWC 4 cut(s) 17, 704, 1911, 2917
Alw26I GTCTC 1 cut(s) 659
Alw44I GTGCAC 2 cut(s) 700, 2913
AlwI GGATC 5 cut(s) 329, 581, 596, 1157, 2477
AlwNI CAGNNNCTG 1 cut(s) 1166
Ama87I CYCGRG 1 cut(s) 2680
AoxI GGCC 5 cut(s) 6, 1580, 2683, 2761, 2887
ApaLI GTGCAC 2 cut(s) 700, 2913
ApeKI GCWGC 8 cut(s) 929, 1096, 1739, 2015, 2018, 2061, 2164, 2426
ApoI RAATTY 6 cut(s) 1177, 1291, 1943, 2523, 2803, 2811
AsiGI ACCGGT 1 cut(s) 2516
Asp700I GAANNNNTTC 5 cut(s) 113, 116, 803, 2004, 2193
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 5 cut(s) 1329, 1901, 1926, 2377, 2683
AsuC2I CCSGG 3 cut(s) 106, 1899, 2404
AsuHPI GGTGA 7 cut(s) 179, 1634, 1844, 1901, 2531, 2566, 2613
AsuII TTCGAA 2 cut(s) 2135, 2200
AsuNHI GCTAGC 1 cut(s) 2167
AvaI CYCGRG 1 cut(s) 2680
AvaII GGWCC 4 cut(s) 1329, 1901, 1926, 2377
AxyI CCTNAGG 2 cut(s) 2391, 2875
BaeGI GKGCMC 2 cut(s) 704, 2917
BaeI ACNNNNGTAYC 2 cut(s) 1605, 1638
BanII GRGCYC 4 cut(s) 17, 61, 177, 1911
BarI GAAGNNNNNNTAC 2 cut(s) 1380, 1412
Bbv12I GWGCWC 4 cut(s) 17, 704, 1911, 2917
BbvI GCAGC 8 cut(s) 916, 1108, 1726, 2002, 2030, 2048, 2151, 2438
BccI CCATC 3 cut(s) 257, 1243, 1979
BceAI ACGGC 1 cut(s) 2156
BciT130I CCWGG 5 cut(s) 534, 794, 1167, 1267, 1376
BciVI GTATCC 1 cut(s) 2464
BclI TGATCA 1 cut(s) 1630
BcnI CCSGG 3 cut(s) 106, 1899, 2404
BcoDI GTCTC 1 cut(s) 659
BfaI CTAG 5 cut(s) 891, 926, 1410, 1419, 2168
BfmI CTRYAG 5 cut(s) 1545, 1608, 2016, 2062, 2244
BfuAI ACCTGC 3 cut(s) 211, 1436, 2725
BfuI GTATCC 1 cut(s) 2464
BglI GCCNNNNNGGC 1 cut(s) 25
BglII AGATCT 2 cut(s) 1110, 2366
BisI GCNGC 9 cut(s) 9, 930, 1097, 1740, 2016, 2019, 2062, 2165, 2427
BlsI GCNGC 9 cut(s) 10, 931, 1098, 1741, 2017, 2020, 2063, 2166, 2428
Bme1390I CCNGG 8 cut(s) 106, 534, 794, 1167, 1267, 1376, 1899, 2404
Bme18I GGWCC 4 cut(s) 1329, 1901, 1926, 2377
BmeT110I CYCGRG 1 cut(s) 2680
BmgT120I GGNCC 5 cut(s) 1329, 1901, 1926, 2377, 2683
BmiI GGNNCC 7 cut(s) 29, 774, 999, 1153, 1927, 2346, 2836
BmrFI CCNGG 8 cut(s) 106, 534, 794, 1167, 1267, 1376, 1899, 2404
BmrI ACTGGG 1 cut(s) 1974
BmsI GCATC 7 cut(s) 437, 1273, 1432, 1487, 1508, 1663, 2924
BmtI GCTAGC 1 cut(s) 2171
BmuI ACTGGG 1 cut(s) 1974
BpmI CTGGAG 6 cut(s) 61, 187, 681, 815, 1099, 1249
Bpu10I CCTNAGC 2 cut(s) 2160, 2929
Bpu14I TTCGAA 2 cut(s) 2135, 2200
BpuEI CTTGAG 1 cut(s) 2357
BpuMI CCSGG 3 cut(s) 106, 1899, 2404
BsaBI GATNNNNATC 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 7 cut(s) 67, 123, 533, 1008, 1065, 2402, 2679
BsaWI WCCGGW 1 cut(s) 2516
BsaXI ACNNNNNCTCC 4 cut(s) 308, 338, 2050, 2080
Bse118I RCCGGY 2 cut(s) 35, 2516
Bse21I CCTNAGG 2 cut(s) 2391, 2875
Bse3DI GCAATG 1 cut(s) 2752
Bse8I GATNNNNATC 1 cut(s) 89
BseBI CCWGG 5 cut(s) 534, 794, 1167, 1267, 1376
BseDI CCNNGG 7 cut(s) 67, 123, 533, 1008, 1065, 2402, 2679
BseGI GGATG 3 cut(s) 643, 1254, 2939
BseJI GATNNNNATC 1 cut(s) 89
BseMI GCAATG 1 cut(s) 2752
BseMII CTCAG 8 cut(s) 377, 774, 1496, 1866, 2151, 2382, 2666, 2889
BseRI GAGGAG 1 cut(s) 2408
BseSI GKGCMC 2 cut(s) 704, 2917
BseXI GCAGC 8 cut(s) 916, 1108, 1726, 2002, 2030, 2048, 2151, 2438
BseYI CCCAGC 2 cut(s) 1346, 2147
BsgI GTGCAG 3 cut(s) 915, 1776, 2238
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 5 cut(s) 8, 1582, 2685, 2763, 2889
BshTI ACCGGT 1 cut(s) 2516
BsiHKAI GWGCWC 4 cut(s) 17, 704, 1911, 2917
BsiHKCI CYCGRG 1 cut(s) 2680
BsiSI CCGG 7 cut(s) 20, 36, 105, 1899, 2404, 2517, 2721
BslFI GGGAC 1 cut(s) 1001
BsmAI GTCTC 1 cut(s) 659
BsmFI GGGAC 1 cut(s) 1001
BsnI GGCC 5 cut(s) 8, 1582, 2685, 2763, 2889
Bso31I GGTCTC 1 cut(s) 659
BsoBI CYCGRG 1 cut(s) 2680
Bsp119I TTCGAA 2 cut(s) 2135, 2200
Bsp1286I GDGCHC 6 cut(s) 17, 61, 177, 704, 1911, 2917
Bsp1407I TGTACA 2 cut(s) 350, 1516
BspACI CCGC 7 cut(s) 9, 33, 439, 530, 646, 2234, 2798
BspANI GGCC 5 cut(s) 8, 1582, 2685, 2763, 2889
BspCNI CTCAG 8 cut(s) 378, 775, 1495, 1867, 2152, 2383, 2665, 2888
BspFNI CGCG 1 cut(s) 11
BspHI TCATGA 1 cut(s) 2305
BspLI GGNNCC 7 cut(s) 29, 774, 999, 1153, 1927, 2346, 2836
BspMAI CTGCAG 2 cut(s) 2020, 2066
BspMI ACCTGC 3 cut(s) 211, 1436, 2725
BspOI GCTAGC 1 cut(s) 2171
BspPI GGATC 5 cut(s) 329, 581, 596, 1157, 2477
BspQI GCTCTTC 3 cut(s) 165, 1401, 1845
BspT104I TTCGAA 2 cut(s) 2135, 2200
BspTNI GGTCTC 1 cut(s) 659
BsrBI CCGCTC 1 cut(s) 2236
BsrDI GCAATG 1 cut(s) 2752
BsrFI RCCGGY 2 cut(s) 35, 2516
BsrGI TGTACA 2 cut(s) 350, 1516
BssAI RCCGGY 2 cut(s) 35, 2516
BssECI CCNNGG 7 cut(s) 67, 123, 533, 1008, 1065, 2402, 2679
BssNAI GTATAC 1 cut(s) 144
BssT1I CCWWGG 2 cut(s) 123, 1065
Bst1107I GTATAC 1 cut(s) 144
Bst2UI CCWGG 5 cut(s) 534, 794, 1167, 1267, 1376
Bst6I CTCTTC 5 cut(s) 165, 577, 1401, 1644, 1845
BstAPI GCANNNNNTGC 1 cut(s) 447
BstAUI TGTACA 2 cut(s) 350, 1516
BstBI TTCGAA 2 cut(s) 2135, 2200
BstC8I GCNNGC 6 cut(s) 13, 17, 37, 2145, 2169, 2796
BstDSI CCRYGG 1 cut(s) 67
BstENI CCTNNNNNAGG 2 cut(s) 1422, 2928
BstF5I GGATG 3 cut(s) 643, 1254, 2939
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 402
BstMAI GTCTC 1 cut(s) 659
BstMWI GCNNNNNNNGC 6 cut(s) 25, 447, 1004, 1451, 2261, 2934
BstNI CCWGG 5 cut(s) 534, 794, 1167, 1267, 1376
BstNSI RCATGY 4 cut(s) 700, 902, 1499, 1885
BstSCI CCNGG 8 cut(s) 104, 532, 792, 1165, 1265, 1374, 1897, 2402
BstSFI CTRYAG 5 cut(s) 1545, 1608, 2016, 2062, 2244
BstSLI GKGCMC 2 cut(s) 704, 2917
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 8 cut(s) 916, 1108, 1726, 2002, 2030, 2048, 2151, 2438
BstX2I RGATCY 4 cut(s) 586, 1110, 1162, 2366
BstXI CCANNNNNNTGG 2 cut(s) 68, 267
BstYI RGATCY 4 cut(s) 586, 1110, 1162, 2366
BstZ17I GTATAC 1 cut(s) 144
Bsu36I CCTNAGG 2 cut(s) 2391, 2875
BsuI GTATCC 1 cut(s) 2464
BsuRI GGCC 5 cut(s) 8, 1582, 2685, 2763, 2889
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 3 cut(s) 643, 1254, 2939
BtsI GCAGTG 4 cut(s) 471, 1209, 2603, 2830
BveI ACCTGC 3 cut(s) 211, 1436, 2725
Cac8I GCNNGC 6 cut(s) 13, 17, 37, 2145, 2169, 2796
CaiI CAGNNNCTG 1 cut(s) 1166
CciI TCATGA 1 cut(s) 2305
CfoI GCGC 1 cut(s) 402
Cfr10I RCCGGY 2 cut(s) 35, 2516
Cfr13I GGNCC 5 cut(s) 1329, 1901, 1926, 2377, 2683
CseI GACGC 1 cut(s) 34
Csp6I GTAC 6 cut(s) 319, 351, 967, 1517, 1550, 2316
CspAI ACCGGT 1 cut(s) 2516
CviQI GTAC 6 cut(s) 319, 351, 967, 1517, 1550, 2316
EaeI YGGCCR 2 cut(s) 6, 2761
Eam1104I CTCTTC 5 cut(s) 165, 577, 1401, 1644, 1845
EarI CTCTTC 5 cut(s) 165, 577, 1401, 1644, 1845
Ecl136II GAGCTC 2 cut(s) 15, 1909
Eco130I CCWWGG 2 cut(s) 123, 1065
Eco147I AGGCCT 1 cut(s) 2889
Eco24I GRGCYC 4 cut(s) 17, 61, 177, 1911
Eco31I GGTCTC 1 cut(s) 659
Eco47I GGWCC 4 cut(s) 1329, 1901, 1926, 2377
Eco53kI GAGCTC 2 cut(s) 15, 1909
Eco57I CTGAAG 4 cut(s) 1206, 1440, 1683, 1869
Eco81I CCTNAGG 2 cut(s) 2391, 2875
Eco88I CYCGRG 1 cut(s) 2680
EcoICRI GAGCTC 2 cut(s) 15, 1909
EcoNI CCTNNNNNAGG 2 cut(s) 1422, 2928
EcoRI GAATTC 1 cut(s) 1943
EcoRII CCWGG 5 cut(s) 532, 792, 1165, 1265, 1374
EcoT14I CCWWGG 2 cut(s) 123, 1065
EcoT22I ATGCAT 2 cut(s) 452, 1501
EcoT38I GRGCYC 4 cut(s) 17, 61, 177, 1911
ErhI CCWWGG 2 cut(s) 123, 1065
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 1 cut(s) 1001
FauI CCCGC 2 cut(s) 40, 2791
FauNDI CATATG 1 cut(s) 1239
FbaI TGATCA 1 cut(s) 1630
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 9 cut(s) 9, 930, 1097, 1740, 2016, 2019, 2062, 2165, 2427
FokI GGATG 3 cut(s) 650, 1261, 2946
FriOI GRGCYC 4 cut(s) 17, 61, 177, 1911
Fsp4HI GCNGC 9 cut(s) 9, 930, 1097, 1740, 2016, 2019, 2062, 2165, 2427
FspBI CTAG 5 cut(s) 891, 926, 1410, 1419, 2168
GlaI GCGC 1 cut(s) 401
GluI GCNGC 9 cut(s) 9, 930, 1097, 1740, 2016, 2019, 2062, 2165, 2427
GsaI CCCAGC 2 cut(s) 1350, 2151
GsuI CTGGAG 6 cut(s) 61, 187, 681, 815, 1099, 1249
HaeIII GGCC 5 cut(s) 8, 1582, 2685, 2763, 2889
HapII CCGG 7 cut(s) 20, 36, 105, 1899, 2404, 2517, 2721
HgaI GACGC 1 cut(s) 34
HhaI GCGC 1 cut(s) 402
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HincII GTYRAC 3 cut(s) 720, 1773, 2843
HindII GTYRAC 3 cut(s) 720, 1773, 2843
HpaI GTTAAC 1 cut(s) 1773
HpaII CCGG 7 cut(s) 20, 36, 105, 1899, 2404, 2517, 2721
HphI GGTGA 7 cut(s) 179, 1634, 1844, 1901, 2531, 2566, 2613
Hpy99I CGWCG 2 cut(s) 1329, 2130
HpyAV CCTTC 4 cut(s) 1297, 1960, 2340, 2416
HpyCH4IV ACGT 2 cut(s) 946, 1823
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 447, 1004, 1451, 2261, 2934
HpySE526I ACGT 2 cut(s) 946, 1823
HspAI GCGC 1 cut(s) 400
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 1630
KspAI GTTAAC 1 cut(s) 1773
LguI GCTCTTC 3 cut(s) 165, 1401, 1845
LmnI GCTCC 6 cut(s) 33, 42, 778, 1003, 2058, 2241
Lsp1109I GCAGC 8 cut(s) 916, 1108, 1726, 2002, 2030, 2048, 2151, 2438
LweI GCATC 7 cut(s) 437, 1273, 1432, 1487, 1508, 1663, 2924
MaeI CTAG 5 cut(s) 891, 926, 1410, 1419, 2168
MaeII ACGT 2 cut(s) 946, 1823
MaeIII GTNAC 8 cut(s) 326, 422, 469, 1832, 2445, 2552, 2572, 2639
MbiI CCGCTC 1 cut(s) 2236
MfeI CAATTG 1 cut(s) 2334
MflI RGATCY 4 cut(s) 586, 1110, 1162, 2366
MhlI GDGCHC 6 cut(s) 17, 61, 177, 704, 1911, 2917
MlyI GAGTC 6 cut(s) 1198, 1226, 1370, 1444, 1568, 1811
MmeI TCCRAC 7 cut(s) 78, 289, 1025, 1226, 1953, 2108, 2391
Mph1103I ATGCAT 2 cut(s) 452, 1501
MroNI GCCGGC 1 cut(s) 35
MroXI GAANNNNTTC 5 cut(s) 113, 116, 803, 2004, 2193
MseI TTAA 4 cut(s) 1031, 1223, 1772, 2505
MslI CAYNNNNRTG 2 cut(s) 476, 1032
MspA1I CMGCKG 2 cut(s) 2021, 2429
MspI CCGG 7 cut(s) 20, 36, 105, 1899, 2404, 2517, 2721
MspR9I CCNGG 8 cut(s) 106, 534, 794, 1167, 1267, 1376, 1899, 2404
MunI CAATTG 1 cut(s) 2334
MvaI CCWGG 5 cut(s) 534, 794, 1167, 1267, 1376
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 6 cut(s) 25, 447, 1004, 1451, 2261, 2934
NaeI GCCGGC 1 cut(s) 37
NciI CCSGG 3 cut(s) 106, 1899, 2404
NdeI CATATG 1 cut(s) 1239
NgoMIV GCCGGC 1 cut(s) 35
NheI GCTAGC 1 cut(s) 2167
NlaIV GGNNCC 7 cut(s) 29, 774, 999, 1153, 1927, 2346, 2836
NmeAIII GCCGAG 1 cut(s) 1033
NmuCI GTSAC 6 cut(s) 422, 469, 1832, 2445, 2572, 2639
NsiI ATGCAT 2 cut(s) 452, 1501
NspI RCATGY 4 cut(s) 700, 902, 1499, 1885
NspV TTCGAA 2 cut(s) 2135, 2200
PagI TCATGA 1 cut(s) 2305
PaqCI CACCTGC 2 cut(s) 211, 2725
PceI AGGCCT 1 cut(s) 2889
PciI ACATGT 2 cut(s) 898, 1881
PciSI GCTCTTC 3 cut(s) 165, 1401, 1845
PdiI GCCGGC 1 cut(s) 37
PdmI GAANNNNTTC 5 cut(s) 113, 116, 803, 2004, 2193
PfeI GAWTC 5 cut(s) 1272, 2249, 2309, 2437, 2757
PflMI CCANNNNNTGG 3 cut(s) 611, 1687, 2615
PfoI TCCNGGA 2 cut(s) 792, 1265
PinAI ACCGGT 1 cut(s) 2516
PkrI GCNGC 9 cut(s) 10, 931, 1098, 1741, 2017, 2020, 2063, 2166, 2428
PleI GAGTC 6 cut(s) 1198, 1226, 1369, 1443, 1567, 1810
PpsI GAGTC 6 cut(s) 1198, 1226, 1369, 1443, 1567, 1810
PscI ACATGT 2 cut(s) 898, 1881
Psp124BI GAGCTC 2 cut(s) 17, 1911
Psp6I CCWGG 5 cut(s) 532, 792, 1165, 1265, 1374
PspFI CCCAGC 2 cut(s) 1346, 2147
PspGI CCWGG 5 cut(s) 532, 792, 1165, 1265, 1374
PspN4I GGNNCC 7 cut(s) 29, 774, 999, 1153, 1927, 2346, 2836
PspPI GGNCC 5 cut(s) 1329, 1901, 1926, 2377, 2683
PstI CTGCAG 2 cut(s) 2020, 2066
PstNI CAGNNNCTG 1 cut(s) 1166
PsuI RGATCY 4 cut(s) 586, 1110, 1162, 2366
PvuII CAGCTG 2 cut(s) 2021, 2429
RsaI GTAC 6 cut(s) 320, 352, 968, 1518, 1551, 2317
RsaNI GTAC 6 cut(s) 319, 351, 967, 1517, 1550, 2316
RseI CAYNNNNRTG 2 cut(s) 476, 1032
SacI GAGCTC 2 cut(s) 17, 1911
SapI GCTCTTC 3 cut(s) 165, 1401, 1845
SaqAI TTAA 4 cut(s) 1031, 1223, 1772, 2505
SatI GCNGC 9 cut(s) 9, 930, 1097, 1740, 2016, 2019, 2062, 2165, 2427
Sau96I GGNCC 5 cut(s) 1329, 1901, 1926, 2377, 2683
SchI GAGTC 6 cut(s) 1198, 1226, 1370, 1444, 1568, 1811
ScrFI CCNGG 8 cut(s) 106, 534, 794, 1167, 1267, 1376, 1899, 2404
SduI GDGCHC 6 cut(s) 17, 61, 177, 704, 1911, 2917
SfaNI GCATC 7 cut(s) 437, 1273, 1432, 1487, 1508, 1663, 2924
SfcI CTRYAG 5 cut(s) 1545, 1608, 2016, 2062, 2244
SfuI TTCGAA 2 cut(s) 2135, 2200
SinI GGWCC 4 cut(s) 1329, 1901, 1926, 2377
SmiMI CAYNNNNRTG 2 cut(s) 476, 1032
SmlI CTYRAG 1 cut(s) 2372
SmoI CTYRAG 1 cut(s) 2372
SseBI AGGCCT 1 cut(s) 2889
SsiI CCGC 7 cut(s) 9, 33, 439, 530, 646, 2234, 2798
SspMI CTAG 5 cut(s) 891, 926, 1410, 1419, 2168
SstI GAGCTC 2 cut(s) 17, 1911
StuI AGGCCT 1 cut(s) 2889
StyD4I CCNGG 8 cut(s) 104, 532, 792, 1165, 1265, 1374, 1897, 2402
StyI CCWWGG 2 cut(s) 123, 1065
TaiI ACGT 2 cut(s) 949, 1826
TaqI TCGA 8 cut(s) 93, 276, 289, 379, 574, 2135, 2200, 2433
TaqII GACCGA 1 cut(s) 1317
TatI WGTACW 5 cut(s) 318, 350, 1516, 1549, 2315
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 5 cut(s) 1272, 2249, 2309, 2437, 2757
Tru1I TTAA 4 cut(s) 1031, 1223, 1772, 2505
Tru9I TTAA 4 cut(s) 1031, 1223, 1772, 2505
TseFI GTSAC 6 cut(s) 422, 469, 1832, 2445, 2572, 2639
TseI GCWGC 8 cut(s) 929, 1096, 1739, 2015, 2018, 2061, 2164, 2426
Tsp45I GTSAC 6 cut(s) 422, 469, 1832, 2445, 2572, 2639
TspDTI ATGAA 8 cut(s) 744, 796, 2274, 2445, 2714, 2775, 2853, 2939
TspGWI ACGGA 5 cut(s) 327, 579, 614, 1244, 2842
Van91I CCANNNNNTGG 3 cut(s) 611, 1687, 2615
VneI GTGCAC 2 cut(s) 700, 2913
VpaK11BI GGWCC 4 cut(s) 1329, 1901, 1926, 2377
XagI CCTNNNNNAGG 2 cut(s) 1422, 2928
XapI RAATTY 6 cut(s) 1177, 1291, 1943, 2523, 2803, 2811
XceI RCATGY 4 cut(s) 700, 902, 1499, 1885
XcmI CCANNNNNNNNNTGG 1 cut(s) 1684
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 5 cut(s) 113, 116, 803, 2004, 2193
XspI CTAG 5 cut(s) 891, 926, 1410, 1419, 2168
Zsp2I ATGCAT 2 cut(s) 452, 1501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.