RchiOBHm_Chr7g0198661

Methyl-CpG binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002094
Physical Location & Seq
Forward (+)
16608129 .. 16615763
7635 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17774

Sequence Viewer

Length: 3120 bp
ATGGTGGTCACGAGCTCCCCTGACTGGCTCCCCGCCGGCTGGAGCGTCCAATTCAGAGCGCAGAACAGGGGTCGCAGAACTGCGATTTACACGGATTTGGAAACTGGGAAAAAGTTCTTCTCCAAGGACGATGTTATACGCTATACTAAAAAAGCCACCAACCGTGGTGAAGAGCCTCAGCGTACAAATTCCAAAAAACCCTCAGATAGAAGTCATTCGCAACTGGCAGCAAGCACAGATGAATATCCTGAATGGTTGCCAGATGGTTGGAAAGTCGAATTGAGAACTCGAAAAACTGGAATTCAAGTTGGAAGGGAGTACAAGTGTTACATTGATCCGTTGACGGAATGTACATTTTATTCCAAGCCAGAGGTATTTCGATACCTGAAAACTGTAAAGCGCAAGAGCTGCAAATCCAGAAGTCACAAAATGGTAAACCGCAAGAGATGTATAATCAAAACGAAGACTGTCACTGCCGTGCAGTTGCCAAGCAAAGTTGTGGTTGAGAAGCATAATGTGGAAGATTTACCGCCAGGGTGGATATTGGAGGTCAAAGTTAGAAAGGTTGCAAATCGAATTAGAAGAGATCCGTATTACACTGATCCAGTGCATGGATATGTCTTCCGCTCCAAAAAGGAAGTTATCCGTTACCTACAAACTGGAGAGACCAGTAAACATACAATCAAACCAAAGAGCACGGGCACGGGCACCAATGATCTAAAGTTGACTGATGATGAAATTGCACTGTCATCTGCAACGAAAAAGCAAAAATTGAAGCACCCTGTCACCAGGCAGCAGCTTTCTGAAGCTAAAAAGAGTTATGAACTGAGTGGCCTAGACTTACAAGAAACTGAAAACTCCCAATTGCAAGACAAGAGGCCCGCTGAAAGCATATGTGATTTGGCTCCAGTTACTGAGGTTATTCTAGAGAAGCATTTCACTGAGAATCTGGTTGAAGAATGTGTAGAAACTAAGGGAGACTGTTCCCCAAGATGGTCATCACAGCCAAAAGCTGAACCATCTAGCAAACACGAAGGCAAAAGGTCATCACAACCGCAAGCTGTACTCTCTAACAAACGTGAGGCTAAAAGGTCCAAGTTGCAAGACAAGAGGGTAACTGATGAAAGCAAAGCTGCTTTGGCTCCTTCAGCTGAGGCAGTCCCACATAAGCATTTAAGTGAGAGTGTGGTTGAAAATTGTGCCAAAACCAAGGAAGATTGTTCTCCAAGCAAGTCATTACAGCCAAAAGCTGAACTCGCCAACAAGTGTGAAGATAAAATTGCATTGGTTGGAGTTGATGGCTCCTCAACAGATCCTGGCAAAGTGGTTTTTTCTGAAGATGACCCTGCTTTGACTCCTGCTGCTAACACCTTGAATAAGAACTCACATATGAGTCAGATGGAAAGGAGAAACACAAGGAGATCCCAAATAGATGCAAGAAAATCGAAACAGAATGAAAAGCTTAATTTGCCACGTCGGTCCTCAAAACGACTGGCTGGGCTTGAACCAGAGCCAGTAAATAGCCTGGTTTCCACTGTACAGGCTCTTCAAGTTACAACTAGAAAGTCTAGTAAAGGTGATGGCAGTCGAGATGCCGGTTTGGCTTTAGATGATTTTGTAAATAGAGCATCTCAGCAGCTTGGGGATGCATCAGAAACAGAGGTTGCACATCATACTATAACTGGAATGATATCTACAGTACAAGGAGAGCCAGTGAGCAAGGTCAAGAGGCCATTTGATGGTCTTGAAGTACAGCCTGTAGATACCTTGGTTTCCAATGAACAAACTCTTCACGTTGCAACTAGAAAGTCTAGTAGAGGTGATGCAAGTCGAGATGCGGGTTTGGCTTTGGATGATTTTATCAATGGAGCATCTCTGCAGCTTGGGGATGCATCAGAACCGGAGGCTGCACATGATAGGTTAACTGACATAAACTGTACATCACATGAAGAGTCATTAAACAAGGGCAAGACGTTTCTTGATGACCAAGTGGTTCCTAAAGAGCAACATCATAGGCTGGAACCTGAGAAAATGGATGCTGAGAAGCCTGGACCAGAGCTCTGCTTTCTGTTTGGATCAGACCCATGCTTAGAATTTGCCTTCAAAACCCTTACTGGCGAATTGCCAATAGCTGATGCTGTGGATAACAAATCTATTATGAAACCTGCAGCTGATACAGTGCAAAAAGAGAACACACTTGAAACTGGGCGCTGCAGTAGAAAACCAAGGGCAATTTCGAACAAATCTAAGAAAGCTAAAGAACTTAAGTTACCTCATCGGACTTCAAAACGGCTGGCTGGGCTTGAACCTGAGCTGCTGGCCAAACCTATGTCCAGTGAAAGAGGTCTTCGAAATGCAACGGCAAAGTCCTGTAGAAGTAAAGCCCTCCAAGCTGTAGATTCTGCACATGAATCATCTCAGCAGTTTGAGGATGGGCAAGAGATCGAGGTTGCTCATGATTTGTGTACTGTTATAAACCCTTCATTTCATGAGGAATCATTGAACAAGATTGAGAAATCTCTCAAGGACGAACCTCTTCCTGAGGAGAAACCCCGTGAGTTGGAAATTGGGAAGGCAGCTGTTGAGAATCCAGTGTCACAGTTTTCATTCCCTTTCCTGGATACTTGGTCTGATCCATGCCTTGATTTTGCATTTAAGACCCTTACAGGTGAAATTCCGATAGAGGATGATTTGTTTCAGGGTTACTTTCAGGAAAACCTTGTCACCTCTCACAACCAAAGAGATAGCTTGGCACTGCCAGATTTTGGCTCACCTAATGTTTTCCAAAGTGACATATCACCTCAGTTCGATGCACCAGAACAGTCTGTCTCGGGCCAATTGTTTCCCATGAATTCTTCTTTGCCACCTGCCGGAAATGTGAGCTTGTCAAATTGCAATGGGGTTGGGTTCGGCCAACAACTGGCCACGAATTCTTCATTTCTGCCTGCAGGAAATTTGAGATTTTCAAGTGGCAGTGGGGTTGGTTCAGGCCAACATTTATCCGTGAATTCTTCATTCTTACCTGCTGGAAATGTGGGCCAGGCAAATTGGAGTGGGATTGGTACACAGAACCCTTGCTTAGGAAGCAAAAAGGATTTTCATGGGAAGGTGAAATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBD

Protein Analysis

1039

Amino Acids

114.84

Weight (kDa)

8.45

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 176 - 222 3e-06 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2474
AarI CACCTGC 1 cut(s) 2875
Acc36I ACCTGC 3 cut(s) 2173, 2875, 3031
AccB1I GGYRCC 1 cut(s) 707
AccB7I CCANNNNNTGG 4 cut(s) 611, 1739, 2765, 2920
AccBSI CCGCTC 1 cut(s) 627
AciI CCGC 7 cut(s) 33, 439, 530, 625, 882, 1055, 1838
AclWI GGATC 7 cut(s) 329, 581, 596, 1307, 1416, 2083, 2627
AcoI YGGCCR 3 cut(s) 2319, 2911, 2922
AcsI RAATTY 8 cut(s) 187, 300, 2093, 2673, 2851, 2929, 2953, 3007
AcuI CTGAAG 3 cut(s) 825, 1131, 1356
AflII CTTAAG 1 cut(s) 2264
AjiI CACGTC 1 cut(s) 1475
AjnI CCWGG 7 cut(s) 532, 788, 1315, 1524, 2047, 2616, 3039
AjuI GAANNNNNNNTTGG 2 cut(s) 2218, 2250
AleI CACNNNNGTG 1 cut(s) 476
Alw21I GWGCWC 3 cut(s) 17, 698, 2061
Alw26I GTCTC 3 cut(s) 659, 972, 2833
AlwI GGATC 7 cut(s) 329, 581, 596, 1307, 1416, 2083, 2627
AlwNI CAGNNNCTG 2 cut(s) 914, 1316
Ama87I CYCGRG 1 cut(s) 2830
AoxI GGCC 9 cut(s) 832, 878, 1730, 2319, 2833, 2911, 2922, 2989, 3037
ApoI RAATTY 8 cut(s) 187, 300, 2093, 2673, 2851, 2929, 2953, 3007
Asp700I GAANNNNTTC 4 cut(s) 113, 214, 935, 2535
AspLEI GCGC 3 cut(s) 61, 402, 2211
AspS9I GGNCC 6 cut(s) 879, 1092, 1479, 2051, 2833, 3037
AsuHPI GGTGA 8 cut(s) 179, 778, 1589, 1832, 2681, 2716, 2763, 2790
AsuII TTCGAA 2 cut(s) 2237, 2350
AvaI CYCGRG 1 cut(s) 2830
AvaII GGWCC 3 cut(s) 1092, 1479, 2051
AxyI CCTNAGG 1 cut(s) 2541
BaeGI GKGCMC 2 cut(s) 704, 710
BaeI ACNNNNGTAYC 2 cut(s) 1755, 1788
BalI TGGCCA 2 cut(s) 2321, 2924
BanI GGYRCC 1 cut(s) 707
BanII GRGCYC 2 cut(s) 17, 2061
BarI GAAGNNNNNNTAC 2 cut(s) 1530, 1562
BauI CACGAG 1 cut(s) 10
BbsI GAAGAC 3 cut(s) 470, 613, 2339
Bbv12I GWGCWC 3 cut(s) 17, 698, 2061
BbvCI CCTCAGC 2 cut(s) 177, 1152
BccI CCATC 8 cut(s) 257, 987, 1027, 1292, 1393, 1574, 1733, 2426
BceAI ACGGC 3 cut(s) 461, 2306, 2376
BcgI CGANNNNNNTGC 2 cut(s) 1425, 1459
BciT130I CCWGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
BciVI GTATCC 1 cut(s) 2614
BcoDI GTCTC 3 cut(s) 659, 972, 2833
BfaI CTAG 7 cut(s) 836, 926, 1023, 1560, 1569, 1803, 1812
BfmI CTRYAG 8 cut(s) 1695, 1758, 1877, 2166, 2212, 2371, 2394, 2946
BfoI RGCGCY 1 cut(s) 2212
BfrI CTTAAG 1 cut(s) 2264
BfuAI ACCTGC 3 cut(s) 2173, 2875, 3031
BfuI GTATCC 1 cut(s) 2614
BglI GCCNNNNNGGC 1 cut(s) 1601
Bme1390I CCNGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
Bme18I GGWCC 3 cut(s) 1092, 1479, 2051
BmeT110I CYCGRG 1 cut(s) 2830
BmgBI CACGTC 1 cut(s) 1475
BmgT120I GGNCC 6 cut(s) 879, 1092, 1479, 2051, 2833, 3037
BmiI GGNNCC 7 cut(s) 29, 709, 906, 1143, 1303, 1995, 2022
BmrFI CCNGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
BmrI ACTGGG 2 cut(s) 114, 2214
BmuI ACTGGG 2 cut(s) 114, 2214
BpiI GAAGAC 3 cut(s) 470, 613, 2339
BpmI CTGGAG 3 cut(s) 61, 681, 891
Bpu10I CCTNAGC 4 cut(s) 177, 1152, 2310, 3079
Bpu14I TTCGAA 2 cut(s) 2237, 2350
BpuEI CTTGAG 1 cut(s) 2507
BsaBI GATNNNNATC 2 cut(s) 243, 997
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 6 cut(s) 123, 163, 533, 1209, 1767, 2225
BsaWI WCCGGW 1 cut(s) 1900
BsaXI ACNNNNNCTCC 4 cut(s) 308, 338, 1698, 1728
Bse118I RCCGGY 2 cut(s) 35, 1595
Bse21I CCTNAGG 1 cut(s) 2541
Bse3DI GCAATG 1 cut(s) 2902
Bse8I GATNNNNATC 2 cut(s) 243, 997
BseBI CCWGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
BseDI CCNNGG 6 cut(s) 123, 163, 533, 1209, 1767, 2225
BseGI GGATG 6 cut(s) 1651, 1858, 1894, 2041, 2437, 2692
BseJI GATNNNNATC 2 cut(s) 243, 997
BseMI GCAATG 1 cut(s) 2902
BseRI GAGGAG 2 cut(s) 1294, 2558
BseSI GKGCMC 2 cut(s) 704, 710
BseYI CCCAGC 2 cut(s) 1496, 2297
BsgI GTGCAG 3 cut(s) 500, 1893, 2388
BshFI GGCC 9 cut(s) 834, 880, 1732, 2321, 2835, 2913, 2924, 2991, 3039
BshNI GGYRCC 1 cut(s) 707
BsiHKAI GWGCWC 3 cut(s) 17, 698, 2061
BsiHKCI CYCGRG 1 cut(s) 2830
BsiSI CCGG 4 cut(s) 36, 1596, 1901, 2871
BslFI GGGAC 1 cut(s) 1145
BsmAI GTCTC 3 cut(s) 659, 972, 2833
BsmFI GGGAC 1 cut(s) 1145
BsnI GGCC 9 cut(s) 834, 880, 1732, 2321, 2835, 2913, 2924, 2991, 3039
Bso31I GGTCTC 1 cut(s) 659
BsoBI CYCGRG 1 cut(s) 2830
Bsp119I TTCGAA 2 cut(s) 2237, 2350
Bsp1286I GDGCHC 5 cut(s) 17, 698, 704, 710, 2061
Bsp1407I TGTACA 3 cut(s) 350, 1537, 1937
Bsp143I GATC 9 cut(s) 334, 586, 601, 715, 1312, 1421, 2075, 2442, 2632
BspACI CCGC 7 cut(s) 33, 439, 530, 625, 882, 1055, 1838
BspANI GGCC 9 cut(s) 834, 880, 1732, 2321, 2835, 2913, 2924, 2991, 3039
BspHI TCATGA 2 cut(s) 2455, 2488
BspLI GGNNCC 7 cut(s) 29, 709, 906, 1143, 1303, 1995, 2022
BspMAI CTGCAG 4 cut(s) 1881, 2170, 2216, 2950
BspMI ACCTGC 3 cut(s) 2173, 2875, 3031
BspPI GGATC 7 cut(s) 329, 581, 596, 1307, 1416, 2083, 2627
BspQI GCTCTTC 2 cut(s) 165, 1551
BspT104I TTCGAA 2 cut(s) 2237, 2350
BspT107I GGYRCC 1 cut(s) 707
BspTI CTTAAG 1 cut(s) 2264
BspTNI GGTCTC 1 cut(s) 659
BsrBI CCGCTC 1 cut(s) 627
BsrDI GCAATG 1 cut(s) 2902
BsrFI RCCGGY 2 cut(s) 35, 1595
BsrGI TGTACA 3 cut(s) 350, 1537, 1937
BssAI RCCGGY 2 cut(s) 35, 1595
BssECI CCNNGG 6 cut(s) 123, 163, 533, 1209, 1767, 2225
BssMI GATC 9 cut(s) 334, 586, 601, 715, 1312, 1421, 2075, 2442, 2632
BssSI CACGAG 1 cut(s) 10
BssT1I CCWWGG 4 cut(s) 123, 1209, 1767, 2225
Bst2BI CACGAG 1 cut(s) 10
Bst2UI CCWGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
Bst6I CTCTTC 6 cut(s) 165, 577, 1551, 1794, 1944, 2541
BstAFI CTTAAG 1 cut(s) 2264
BstAPI GCANNNNNTGC 1 cut(s) 408
BstAUI TGTACA 3 cut(s) 350, 1537, 1937
BstBI TTCGAA 2 cut(s) 2237, 2350
BstC8I GCNNGC 7 cut(s) 37, 232, 882, 1059, 2295, 2319, 2946
BstDSI CCRYGG 1 cut(s) 163
BstENI CCTNNNNNAGG 1 cut(s) 3078
BstF5I GGATG 6 cut(s) 1651, 1858, 1894, 2041, 2437, 2692
BstH2I RGCGCY 1 cut(s) 2212
BstHHI GCGC 3 cut(s) 61, 402, 2211
BstKTI GATC 9 cut(s) 337, 589, 604, 718, 1315, 1424, 2078, 2445, 2635
BstMAI GTCTC 3 cut(s) 659, 972, 2833
BstMBI GATC 9 cut(s) 334, 586, 601, 715, 1312, 1421, 2075, 2442, 2632
BstNI CCWGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
BstSCI CCNGG 7 cut(s) 532, 788, 1315, 1524, 2047, 2616, 3039
BstSFI CTRYAG 8 cut(s) 1695, 1758, 1877, 2166, 2212, 2371, 2394, 2946
BstSLI GKGCMC 2 cut(s) 704, 710
BstV2I GAAGAC 3 cut(s) 470, 613, 2339
BstX2I RGATCY 3 cut(s) 586, 1312, 1421
BstXI CCANNNNNNTGG 1 cut(s) 267
BstYI RGATCY 3 cut(s) 586, 1312, 1421
Bsu36I CCTNAGG 1 cut(s) 2541
BsuI GTATCC 1 cut(s) 2614
BsuRI GGCC 9 cut(s) 834, 880, 1732, 2321, 2835, 2913, 2924, 2991, 3039
BtgI CCRYGG 1 cut(s) 163
BtrI CACGTC 1 cut(s) 1475
BtsCI GGATG 6 cut(s) 1651, 1858, 1894, 2041, 2437, 2692
BtsI GCAGTG 3 cut(s) 471, 2753, 2980
BveI ACCTGC 3 cut(s) 2173, 2875, 3031
Cac8I GCNNGC 7 cut(s) 37, 232, 882, 1059, 2295, 2319, 2946
CaiI CAGNNNCTG 2 cut(s) 914, 1316
CciI TCATGA 2 cut(s) 2455, 2488
CfoI GCGC 3 cut(s) 61, 402, 2211
Cfr10I RCCGGY 2 cut(s) 35, 1595
Cfr13I GGNCC 6 cut(s) 879, 1092, 1479, 2051, 2833, 3037
CseI GACGC 1 cut(s) 34
DpnI GATC 9 cut(s) 336, 588, 603, 717, 1314, 1423, 2077, 2444, 2634
DpnII GATC 9 cut(s) 334, 586, 601, 715, 1312, 1421, 2075, 2442, 2632
EaeI YGGCCR 3 cut(s) 2319, 2911, 2922
Eam1104I CTCTTC 6 cut(s) 165, 577, 1551, 1794, 1944, 2541
EarI CTCTTC 6 cut(s) 165, 577, 1551, 1794, 1944, 2541
Ecl136II GAGCTC 2 cut(s) 15, 2059
Eco130I CCWWGG 4 cut(s) 123, 1209, 1767, 2225
Eco24I GRGCYC 2 cut(s) 17, 2061
Eco31I GGTCTC 1 cut(s) 659
Eco32I GATATC 1 cut(s) 1692
Eco47I GGWCC 3 cut(s) 1092, 1479, 2051
Eco53kI GAGCTC 2 cut(s) 15, 2059
Eco57I CTGAAG 3 cut(s) 825, 1131, 1356
Eco81I CCTNAGG 1 cut(s) 2541
Eco88I CYCGRG 1 cut(s) 2830
EcoICRI GAGCTC 2 cut(s) 15, 2059
EcoNI CCTNNNNNAGG 1 cut(s) 3078
EcoRI GAATTC 4 cut(s) 300, 2851, 2929, 3007
EcoRII CCWGG 7 cut(s) 532, 788, 1315, 1524, 2047, 2616, 3039
EcoRV GATATC 1 cut(s) 1692
EcoT14I CCWWGG 4 cut(s) 123, 1209, 1767, 2225
EcoT22I ATGCAT 2 cut(s) 1651, 1894
EcoT38I GRGCYC 2 cut(s) 17, 2061
ErhI CCWWGG 4 cut(s) 123, 1209, 1767, 2225
FaqI GGGAC 1 cut(s) 1145
FauI CCCGC 3 cut(s) 40, 889, 1831
FauNDI CATATG 2 cut(s) 893, 1389
FokI GGATG 6 cut(s) 1658, 1865, 1901, 2048, 2444, 2699
FriOI GRGCYC 2 cut(s) 17, 2061
FspBI CTAG 7 cut(s) 836, 926, 1023, 1560, 1569, 1803, 1812
GlaI GCGC 3 cut(s) 60, 401, 2210
GsaI CCCAGC 2 cut(s) 1500, 2301
GsuI CTGGAG 3 cut(s) 61, 681, 891
HaeII RGCGCY 1 cut(s) 2212
HaeIII GGCC 9 cut(s) 834, 880, 1732, 2321, 2835, 2913, 2924, 2991, 3039
HapII CCGG 4 cut(s) 36, 1596, 1901, 2871
HgaI GACGC 1 cut(s) 34
HhaI GCGC 3 cut(s) 61, 402, 2211
Hin6I GCGC 3 cut(s) 59, 400, 2209
HinP1I GCGC 3 cut(s) 59, 400, 2209
HincII GTYRAC 3 cut(s) 342, 726, 1923
HindII GTYRAC 3 cut(s) 342, 726, 1923
HindIII AAGCTT 1 cut(s) 1460
HinfI GANTC 8 cut(s) 946, 1354, 1393, 1952, 2399, 2411, 2495, 2587
HpaI GTTAAC 1 cut(s) 1923
HpaII CCGG 4 cut(s) 36, 1596, 1901, 2871
HphI GGTGA 8 cut(s) 179, 778, 1589, 1832, 2681, 2716, 2763, 2790
Hpy166II GTNNAC 7 cut(s) 342, 436, 674, 726, 1923, 2466, 3065
Hpy8I GTNNAC 7 cut(s) 342, 436, 674, 726, 1923, 2466, 3065
Hpy99I CGWCG 1 cut(s) 1479
HpyAV CCTTC 7 cut(s) 306, 1028, 1155, 2110, 2490, 2566, 3100
HpyCH4IV ACGT 4 cut(s) 1078, 1474, 1794, 1973
HpySE526I ACGT 4 cut(s) 1078, 1474, 1794, 1973
HspAI GCGC 3 cut(s) 59, 400, 2209
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
KspAI GTTAAC 1 cut(s) 1923
Kzo9I GATC 9 cut(s) 334, 586, 601, 715, 1312, 1421, 2075, 2442, 2632
LguI GCTCTTC 2 cut(s) 165, 1551
LmnI GCTCC 8 cut(s) 20, 33, 42, 632, 910, 1147, 1307, 1868
MaeI CTAG 7 cut(s) 836, 926, 1023, 1560, 1569, 1803, 1812
MaeII ACGT 4 cut(s) 1078, 1474, 1794, 1973
MalI GATC 9 cut(s) 336, 588, 603, 717, 1314, 1423, 2077, 2444, 2634
MbiI CCGCTC 1 cut(s) 627
MboI GATC 9 cut(s) 334, 586, 601, 715, 1312, 1421, 2075, 2442, 2632
MfeI CAATTG 2 cut(s) 863, 2837
MflI RGATCY 3 cut(s) 586, 1312, 1421
MhlI GDGCHC 5 cut(s) 17, 698, 704, 710, 2061
MlsI TGGCCA 2 cut(s) 2321, 2924
MluNI TGGCCA 2 cut(s) 2321, 2924
MlyI GAGTC 3 cut(s) 1348, 1402, 1961
MmeI TCCRAC 4 cut(s) 248, 289, 1270, 2541
Mox20I TGGCCA 2 cut(s) 2321, 2924
Mph1103I ATGCAT 2 cut(s) 1651, 1894
MroNI GCCGGC 1 cut(s) 35
MroXI GAANNNNTTC 4 cut(s) 113, 214, 935, 2535
MscI TGGCCA 2 cut(s) 2321, 2924
MseI TTAA 6 cut(s) 1175, 1464, 1922, 1958, 2265, 2655
MslI CAYNNNNRTG 3 cut(s) 476, 615, 1176
Msp20I TGGCCA 2 cut(s) 2321, 2924
MspA1I CMGCKG 4 cut(s) 884, 1151, 2171, 2579
MspCI CTTAAG 1 cut(s) 2264
MspI CCGG 4 cut(s) 36, 1596, 1901, 2871
MspR9I CCNGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
MunI CAATTG 2 cut(s) 863, 2837
MvaI CCWGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
NaeI GCCGGC 1 cut(s) 37
NdeI CATATG 2 cut(s) 893, 1389
NdeII GATC 9 cut(s) 334, 586, 601, 715, 1312, 1421, 2075, 2442, 2632
NgoMIV GCCGGC 1 cut(s) 35
NlaIV GGNNCC 7 cut(s) 29, 709, 906, 1143, 1303, 1995, 2022
NmuCI GTSAC 7 cut(s) 7, 422, 469, 784, 2595, 2722, 2789
NsiI ATGCAT 2 cut(s) 1651, 1894
NspV TTCGAA 2 cut(s) 2237, 2350
OliI CACNNNNGTG 1 cut(s) 476
PagI TCATGA 2 cut(s) 2455, 2488
PaqCI CACCTGC 1 cut(s) 2875
PciSI GCTCTTC 2 cut(s) 165, 1551
PdiI GCCGGC 1 cut(s) 37
PdmI GAANNNNTTC 4 cut(s) 113, 214, 935, 2535
PfeI GAWTC 5 cut(s) 946, 2399, 2411, 2495, 2587
PflMI CCANNNNNTGG 4 cut(s) 611, 1739, 2765, 2920
PfoI TCCNGGA 1 cut(s) 2616
PleI GAGTC 3 cut(s) 1348, 1401, 1960
PpsI GAGTC 3 cut(s) 1348, 1401, 1960
PsiI TTATAA 1 cut(s) 2474
Psp124BI GAGCTC 2 cut(s) 17, 2061
Psp6I CCWGG 7 cut(s) 532, 788, 1315, 1524, 2047, 2616, 3039
PspFI CCCAGC 2 cut(s) 1496, 2297
PspGI CCWGG 7 cut(s) 532, 788, 1315, 1524, 2047, 2616, 3039
PspN4I GGNNCC 7 cut(s) 29, 709, 906, 1143, 1303, 1995, 2022
PspPI GGNCC 6 cut(s) 879, 1092, 1479, 2051, 2833, 3037
PsrI GAACNNNNNNTAC 2 cut(s) 2253, 2285
PstI CTGCAG 4 cut(s) 1881, 2170, 2216, 2950
PstNI CAGNNNCTG 2 cut(s) 914, 1316
PsuI RGATCY 3 cut(s) 586, 1312, 1421
PvuII CAGCTG 3 cut(s) 1151, 2171, 2579
RseI CAYNNNNRTG 3 cut(s) 476, 615, 1176
SacI GAGCTC 2 cut(s) 17, 2061
SapI GCTCTTC 2 cut(s) 165, 1551
SaqAI TTAA 6 cut(s) 1175, 1464, 1922, 1958, 2265, 2655
Sau3AI GATC 9 cut(s) 334, 586, 601, 715, 1312, 1421, 2075, 2442, 2632
Sau96I GGNCC 6 cut(s) 879, 1092, 1479, 2051, 2833, 3037
SbfI CCTGCAGG 1 cut(s) 2950
SchI GAGTC 3 cut(s) 1348, 1402, 1961
ScrFI CCNGG 7 cut(s) 534, 790, 1317, 1526, 2049, 2618, 3041
SdaI CCTGCAGG 1 cut(s) 2950
SduI GDGCHC 5 cut(s) 17, 698, 704, 710, 2061
SfcI CTRYAG 8 cut(s) 1695, 1758, 1877, 2166, 2212, 2371, 2394, 2946
SfuI TTCGAA 2 cut(s) 2237, 2350
SinI GGWCC 3 cut(s) 1092, 1479, 2051
SmiMI CAYNNNNRTG 3 cut(s) 476, 615, 1176
SmlI CTYRAG 2 cut(s) 2264, 2522
SmoI CTYRAG 2 cut(s) 2264, 2522
Sse8387I CCTGCAGG 1 cut(s) 2950
SsiI CCGC 7 cut(s) 33, 439, 530, 625, 882, 1055, 1838
SspMI CTAG 7 cut(s) 836, 926, 1023, 1560, 1569, 1803, 1812
SstI GAGCTC 2 cut(s) 17, 2061
StyD4I CCNGG 7 cut(s) 532, 788, 1315, 1524, 2047, 2616, 3039
StyI CCWWGG 4 cut(s) 123, 1209, 1767, 2225
TaiI ACGT 4 cut(s) 1081, 1477, 1797, 1976
TaqII GACCGA 1 cut(s) 1467
TatI WGTACW 8 cut(s) 318, 350, 1063, 1537, 1699, 1750, 1937, 2465
TfiI GAWTC 5 cut(s) 946, 2399, 2411, 2495, 2587
Tru1I TTAA 6 cut(s) 1175, 1464, 1922, 1958, 2265, 2655
Tru9I TTAA 6 cut(s) 1175, 1464, 1922, 1958, 2265, 2655
TseFI GTSAC 7 cut(s) 7, 422, 469, 784, 2595, 2722, 2789
Tsp45I GTSAC 7 cut(s) 7, 422, 469, 784, 2595, 2722, 2789
TspGWI ACGGA 6 cut(s) 107, 327, 359, 579, 635, 2992
Van91I CCANNNNNTGG 4 cut(s) 611, 1739, 2765, 2920
Vha464I CTTAAG 1 cut(s) 2264
VpaK11BI GGWCC 3 cut(s) 1092, 1479, 2051
XagI CCTNNNNNAGG 1 cut(s) 3078
XapI RAATTY 8 cut(s) 187, 300, 2093, 2673, 2851, 2929, 2953, 3007
XbaI TCTAGA 1 cut(s) 925
XmnI GAANNNNTTC 4 cut(s) 113, 214, 935, 2535
XspI CTAG 7 cut(s) 836, 926, 1023, 1560, 1569, 1803, 1812
Zsp2I ATGCAT 2 cut(s) 1651, 1894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.