MD14G1098200.v1.1

Methyl-CpG binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002094
Physical Location & Seq
Reverse (-)
14986384 .. 14994833
8450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1098200.v1.1.491

Sequence Viewer

Length: 3687 bp
ATGGTAGCCAGGAGTTCGCCGGACTGGCTCCCGCCCGGATGGTCTGTCCAATCCAGATCCCAAAAGAGAGGTCGCAAAATTGAGTTTTATACCCATTTGGAAACTGGGAAAAAGTTCTTTTCTAAAGATGATGCTATGCGCTATATCAAAATGCAAAACACTCGTGGTGAAAAGCCTCAGCCAACTTTGGTGAACATCCCAGATCACTCAGAAGAAGTCCCTTCTCAACTTGAAGTGGATCCAACGGAATGTCCTGAATGGTTGCCTAACGGTTGGAAAGTGGAGCTGAGGACACGACAGAGTGGTGTGCAAGTTGGAAAAGAATACAAGTGTTACATTGATCCCTCCGGAGAATGTACATTTTATTCCAAGCCAGAGGTATTTCGATATCTCAAAACTGTAAAACGGAAGAGTTCGAAGTCTGGAACTCTAACTATAAGCAGCAAGAGATGCATAACGGAAAAGAAGGCTGTCACTACCGTGCAGTTGGCAAAAAATGTTTATGTTGAGAAACATCATGTGGAGGATTTACCACCAGGATGGATAAAGGAGATCAAAGTTACAAAGTATGCAAATCGATTTAGAAGAGACCCGTATTACACAGATCCAGACTCTGGATATGTATTCCGCTCCAAAAAAGATGTATTTCGCTATCTTGAAACTGGAGAGATCAGTAAATATGCATTTAAACCCAAGAATATGTGCAATAATGATCTGAAGTTGGTTAATGATGAAATTGCACCATCATCTACAATCAAAATGCAAAAACTTGAGCACCCAGTCACGAGGAGACAGCTATCACAGCCAAACGTTGAGCTCTCTGACAGACATGAAGGTAAAAGGTCATTGCTTGAAGGTGAAGGTTCTGCGAGAGATCCAGGCAAATTGAGTTCTACTCGAGATGAGCCTGTTTTGACTCCTCCTGCTTATACCCTGAATGAGAACAATTCACTTGAACCTGTGATGGAAAAATGCAATGTGAGAAGAGCCCTAGTACACTCGGGCAAATCGAAAAACAAGGAAACGCTTAATTTGTCTCGTCGGTCCTCAAAACGACTTGCTAGGAGCAGGTTAGAACTACCAGAAACTGAAATCTCCAAGTTACTAGACAAAGGAGTATCCTCTGAAAGTGTAGTGGCCTTTGCTTCAACAATTGATGTCATCCAAGAGAAGCATTTAACTGGGAACATACTGGAAAAATGTGCCAAAACTAAGGAAGATTGTTCTCCAGACACTTCGATGGAAAAAAGCTATGTCAGAAGAACCCCAATAGACTCGAGCAAATCCAAAAACAAGGAAATGCTTAATTTCTGCCGTCGGTTCTCAAAACGACTTGCTGGGAGCAGGTTGGAACTACCAGAAACTGAAAGCTCCAAGTTGCTAGACAATAGGGTACCCTCTGAAAATGGACTGGCCTTTGCTTCGACAGCTGGGGTTGTCCAACAGAAAAATTTAACTGGGAACTTGCTTGAAAAATGTGCCAAAACTAAGGAAGATTGTTCCCCAAACACTGTGATGGAAAAAAGCAATGTCAGAAGAACCCTGGTAGACTCGGGCAAATCAAAAAACAAGGAAAAGCTTAATTTGTCTCGCCGGTTTTCAAAACGACTTGCTGGGAGCAGGTTGGAACTACCGGAAACTGAAATTTCGGAGTTGCCAGACAAGATGGCACCCTCAGTAAGTGGAGTTGCCTTTACTATGACAGCTGATGTTGTTCAAGAGAAGCATTTAACTGATAACTTGCTGGAAAAATGTGCCAAAACGAAGGAAGATGGTTCTCCAAGCGGGTCATCATACCCAAAAGCTGAACTCTCGGAGAGACAGGAAGGAGAAAGGTCATTGCTTGAAGTTGTAGGTTCTGTGATAGACCCAGAAAAAATGATGTTTGCTGGAAATGAGCCCGTTTTGACTCCTGCTTCTTACACCCTGAATGAGAATAATTCGCATAAGACTGTTGAGAAAAAAAGCAACGTCAGAAAAGCTCCAATAGGCTCGAGCAAATCAAAAAGCAAGGAAAAACTTATTTTGTCTCGTCGGTCCTCAAAACGACTTGCTGGGCTTGAACCAGAGGAGGTTGCTAACTTGGCTTCCAGTGAACGAGTTGTTCAAGCTCCAACTAGGAAGCCTAGTAAAAGTGATACCAGTCAAGACGTAGGTTTGGCTTCAGATCTTGTAAACAGAGCATCTCAGCAGCTTGGGGCTGCATCAGAAGCAGAGGTTTCACATCATGCTTTAACTGACATAAAATCTATATCACATGAGGACAAGATGCCAATTGATGACCCAGTGGTTCCCAGAGCGCAACAAGAGCTGGAAACTGAGATAATGGATGCTGAGAAGCCAGAGCCAGAGCTCAGCTTTCTGTTTGGTTCAGACCCATGCTTAGAATTCGCATTCAAAACGCTTACAGGGGAATTGCCAATAGCTGATACTGTGGATAACAGATCCATTTTGAGACCTGCAGCTGATATGCTGCATAAAAAAAATTTACTTGACAGTGGGATGGAAAAGAACTGCAGTAGAAAACCAAGGGCTAACAAATCCAAGAAAAATAAAGATCTTAAGTTGTCCAGTTGGTCTTCAAAACAGTATGCTGGGGTTCAACCTGAGCTGCTGGCTAATACTACGTCCAAGGAACGAGCTATCCCAAATGCAACTGAAAATTCATGTCAAAGTATAGCCATCCCCCCGGTGGATTTGGCAGATGAAGCATCTCAACCGCTTGAGATTACACCAGAGGTGAAGCTTGCCAGTCGTGCTTGTACTGCTATATACAATTCAATGCAAGAGGAGACGTCATACAAGAGAGCGAAGGCTCTTGAAGAGCATGCTGTTCCTCAGGAGAAACCCCAGAAGTTTGAAACCGAGAAGGCATATGTTGAGAATCCAGAGCCACAGTTCCCTTTTCCTTTCACGGATTCATGGTCTGACCCATGCCTCGATTTTGCATTCAAGACTCTTACAGGTGCAATTCCATTAGAGGATGATTATTATCAAGGTTACTTCCAAGAAAAACTTGACACATCTCACAATATGGCACTATCAGATTTTGGCTCACCAAGCTTGTTCCGAAGTGATAATTTACCTCAGTTCAATCCACCAGAACAATCTGCTTTTGGCCAACAGTTATCCATGAATTCTGCATTCCTGCCTTCAGGAAATGTGAGCATTCCGAATTGCGGTGGAAGTGGTTCAGGCCAACAGTTATCTAGGAATGCTTCATCCCTCCCAGCAGGAAATGTGAGCATTTCAAATTGCAGCGGGGTTTGTTCAGGCCAACAGTTATCCATGAATTCTGAATTCCTGCCTTCAGGAAATGTGAGCATGCCGAATTGCGGTGGAATTGGTTCAGGCCAACAGTCATCTAGGAATCCTTCATCCCTCCCTCCAGGAAACGTGAGCATGTCAAATTGCAGCGGGGTTTGTTCAGGCCAACAGTTATTTAGGAATCCTACATCCCTCCCTACAGGAAACTTGAGCATTTCAAATTGCAGTGGGGTTTGTTCAGGCCACCAGTTATCCATGAATTCTTCATTTCAGCCCGCTGGAAACATGAGCACATCAAATTTCAGCGGGGTGTGTTCAGGCCAAGAGTTATCTGTAAATTCTTCATTACTGCCTGCCGGAAGCTTGGTTGGTTCGCAGTGGCCTTGCGTAGATGATTATCATGGAAAGGTAAAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBD

Protein Analysis

1229

Amino Acids

135.47

Weight (kDa)

8.53

Isoelectric Point (pI)

54.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 82 - 135 1.3e-07 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2798
Acc36I ACCTGC 4 cut(s) 1059, 1335, 1611, 2467
Acc65I GGTACC 1 cut(s) 1393
AccB1I GGYRCC 2 cut(s) 1393, 1669
AccB7I CCANNNNNTGG 1 cut(s) 614
AccBSI CCGCTC 1 cut(s) 630
AccI GTMKAC 1 cut(s) 1548
AccIII TCCGGA 1 cut(s) 347
AclI AACGTT 1 cut(s) 810
AclWI GGATC 7 cut(s) 51, 233, 246, 335, 599, 869, 2439
AcoI YGGCCR 1 cut(s) 3118
AcuI CTGAAG 4 cut(s) 737, 2148, 3138, 3294
AcyI GRCGYC 1 cut(s) 2795
AfaI GTAC 4 cut(s) 358, 996, 1395, 2764
AfiI CCNNNNNNNGG 4 cut(s) 614, 2692, 3179, 3335
AflII CTTAAG 1 cut(s) 2561
AjnI CCWGG 5 cut(s) 8, 535, 877, 1542, 3388
AleI CACNNNNGTG 1 cut(s) 479
AloI GAACNNNNNNTCC 2 cut(s) 2628, 2660
Alw21I GWGCWC 4 cut(s) 777, 819, 2355, 3560
Alw26I GTCTC 8 cut(s) 582, 784, 1041, 1593, 1813, 2034, 2449, 2786
AlwI GGATC 7 cut(s) 51, 233, 246, 335, 599, 869, 2439
AlwNI CAGNNNCTG 3 cut(s) 614, 1088, 1364
Ama87I CYCGRG 5 cut(s) 897, 1000, 1276, 1552, 1993
Aor13HI TCCGGA 1 cut(s) 347
ApeKI GCWGC 8 cut(s) 441, 2191, 2201, 2462, 2473, 2611, 3258, 3414
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
Asp700I GAANNNNTTC 3 cut(s) 113, 3190, 3346
Asp718I GGTACC 1 cut(s) 1393
AspLEI GCGC 2 cut(s) 141, 2302
AspS9I GGNCC 2 cut(s) 1044, 2037
AsuC2I CCSGG 2 cut(s) 36, 2690
AsuHPI GGTGA 5 cut(s) 179, 202, 869, 2752, 3048
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 5 cut(s) 897, 1000, 1276, 1552, 1993
AvaII GGWCC 2 cut(s) 1044, 2037
AxyI CCTNAGG 1 cut(s) 2838
BalI TGGCCA 1 cut(s) 3120
BamHI GGATCC 1 cut(s) 238
BanI GGYRCC 2 cut(s) 1393, 1669
BanII GRGCYC 4 cut(s) 819, 991, 1902, 2355
BauI CACGAG 2 cut(s) 162, 784
BbsI GAAGAC 1 cut(s) 2571
Bbv12I GWGCWC 4 cut(s) 777, 819, 2355, 3560
BbvCI CCTCAGC 2 cut(s) 177, 287
BbvI GCAGC 8 cut(s) 453, 2188, 2203, 2460, 2474, 2598, 3270, 3426
BceAI ACGGC 1 cut(s) 1299
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 5 cut(s) 10, 537, 879, 1544, 3390
BciVI GTATCC 1 cut(s) 1129
BcnI CCSGG 2 cut(s) 36, 2690
BcoDI GTCTC 8 cut(s) 582, 784, 1041, 1593, 1813, 2034, 2449, 2786
BfaI CTAG 9 cut(s) 992, 1062, 1106, 1382, 2118, 2127, 3210, 3366, 3685
BfmI CTRYAG 3 cut(s) 2460, 2515, 3465
BfrI CTTAAG 1 cut(s) 2561
BfuAI ACCTGC 4 cut(s) 1059, 1335, 1611, 2467
BfuI GTATCC 1 cut(s) 1129
BglI GCCNNNNNGGC 1 cut(s) 25
BglII AGATCT 2 cut(s) 2167, 2557
BisI GCNGC 8 cut(s) 442, 2192, 2202, 2463, 2474, 2612, 3259, 3415
BlpI GCTNAGC 1 cut(s) 2354
BlsI GCNGC 8 cut(s) 443, 2193, 2203, 2464, 2475, 2613, 3260, 3416
Bme1390I CCNGG 7 cut(s) 10, 36, 537, 879, 1544, 2690, 3390
Bme18I GGWCC 2 cut(s) 1044, 2037
BmeT110I CYCGRG 5 cut(s) 897, 1000, 1276, 1552, 1993
BmgT120I GGNCC 2 cut(s) 1044, 2037
BmiI GGNNCC 5 cut(s) 29, 240, 1395, 1671, 2292
BmrFI CCNGG 7 cut(s) 10, 36, 537, 879, 1544, 2690, 3390
BmrI ACTGGG 5 cut(s) 114, 773, 1191, 1467, 2279
BmsI GCATC 7 cut(s) 121, 440, 2192, 2213, 2259, 2320, 2720
BmuI ACTGGG 5 cut(s) 114, 773, 1191, 1467, 2279
BpiI GAAGAC 1 cut(s) 2571
BplI GAGNNNNNCTC 2 cut(s) 880, 912
BpmI CTGGAG 3 cut(s) 684, 1212, 3372
Bpu10I CCTNAGC 3 cut(s) 177, 287, 2607
Bpu1102I GCTNAGC 1 cut(s) 2354
Bpu14I TTCGAA 1 cut(s) 416
BpuEI CTTGAG 3 cut(s) 791, 2744, 3496
BpuMI CCSGG 2 cut(s) 36, 2690
Bsa29I ATCGAT 1 cut(s) 577
BsaBI GATNNNNATC 2 cut(s) 2991, 3663
BsaHI GRCGYC 1 cut(s) 2795
BsaI GGTCTC 2 cut(s) 582, 2449
BsaJI CCNNGG 4 cut(s) 1542, 2528, 2631, 2688
BsaWI WCCGGW 2 cut(s) 347, 1633
Bsc4I CCNNNNNNNGG 4 cut(s) 614, 2692, 3179, 3335
Bse118I RCCGGY 1 cut(s) 1593
Bse21I CCTNAGG 1 cut(s) 2838
Bse3DI GCAATG 4 cut(s) 845, 982, 1534, 1838
Bse8I GATNNNNATC 2 cut(s) 2991, 3663
BseAI TCCGGA 1 cut(s) 347
BseBI CCWGG 5 cut(s) 10, 537, 879, 1544, 3390
BseCI ATCGAT 1 cut(s) 577
BseDI CCNNGG 4 cut(s) 1542, 2528, 2631, 2688
BseJI GATNNNNATC 2 cut(s) 2991, 3663
BseLI CCNNNNNNNGG 4 cut(s) 614, 2692, 3179, 3335
BseMI GCAATG 4 cut(s) 845, 982, 1534, 1838
BseRI GAGGAG 4 cut(s) 802, 909, 2084, 2804
BseXI GCAGC 8 cut(s) 453, 2188, 2203, 2460, 2474, 2598, 3270, 3426
BseYI CCCAGC 6 cut(s) 1337, 1430, 1613, 2054, 2594, 3229
BsgI GTGCAG 1 cut(s) 503
BshNI GGYRCC 2 cut(s) 1393, 1669
BshVI ATCGAT 1 cut(s) 577
BsiHKAI GWGCWC 4 cut(s) 777, 819, 2355, 3560
BsiHKCI CYCGRG 5 cut(s) 897, 1000, 1276, 1552, 1993
BsiSI CCGG 7 cut(s) 20, 36, 348, 1594, 1634, 2690, 3624
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 4 cut(s) 614, 2692, 3179, 3335
BsmAI GTCTC 8 cut(s) 582, 784, 1041, 1593, 1813, 2034, 2449, 2786
BsmBI CGTCTC 1 cut(s) 2786
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 5 cut(s) 2393, 2948, 3143, 3168, 3220
Bso31I GGTCTC 2 cut(s) 582, 2449
BsoBI CYCGRG 5 cut(s) 897, 1000, 1276, 1552, 1993
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 6 cut(s) 777, 819, 991, 1902, 2355, 3560
Bsp13I TCCGGA 1 cut(s) 347
Bsp1407I TGTACA 1 cut(s) 356
Bsp1720I GCTNAGC 1 cut(s) 2354
BspDI ATCGAT 1 cut(s) 577
BspEI TCCGGA 1 cut(s) 347
BspLI GGNNCC 5 cut(s) 29, 240, 1395, 1671, 2292
BspMAI CTGCAG 2 cut(s) 2464, 2519
BspMI ACCTGC 4 cut(s) 1059, 1335, 1611, 2467
BspPI GGATC 7 cut(s) 51, 233, 246, 335, 599, 869, 2439
BspQI GCTCTTC 2 cut(s) 979, 2817
BspT104I TTCGAA 1 cut(s) 416
BspT107I GGYRCC 2 cut(s) 1393, 1669
BspTI CTTAAG 1 cut(s) 2561
BspTNI GGTCTC 2 cut(s) 582, 2449
BsrBI CCGCTC 1 cut(s) 630
BsrDI GCAATG 4 cut(s) 845, 982, 1534, 1838
BsrFI RCCGGY 1 cut(s) 1593
BsrGI TGTACA 1 cut(s) 356
BssAI RCCGGY 1 cut(s) 1593
BssECI CCNNGG 4 cut(s) 1542, 2528, 2631, 2688
BssNI GRCGYC 1 cut(s) 2795
BssSI CACGAG 2 cut(s) 162, 784
BssT1I CCWWGG 2 cut(s) 2528, 2631
Bst2BI CACGAG 2 cut(s) 162, 784
Bst2UI CCWGG 5 cut(s) 10, 537, 879, 1544, 3390
Bst6I CTCTTC 4 cut(s) 404, 580, 979, 2817
BstACI GRCGYC 1 cut(s) 2795
BstAFI CTTAAG 1 cut(s) 2561
BstAPI GCANNNNNTGC 1 cut(s) 450
BstAUI TGTACA 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 6 cut(s) 2616, 2748, 2829, 3326, 3543, 3621
BstHHI GCGC 2 cut(s) 141, 2302
BstMAI GTCTC 8 cut(s) 582, 784, 1041, 1593, 1813, 2034, 2449, 2786
BstNI CCWGG 5 cut(s) 10, 537, 879, 1544, 3390
BstNSI RCATGY 3 cut(s) 2831, 3328, 3406
BstSCI CCNGG 7 cut(s) 8, 34, 535, 877, 1542, 2688, 3388
BstSFI CTRYAG 3 cut(s) 2460, 2515, 3465
BstV1I GCAGC 8 cut(s) 453, 2188, 2203, 2460, 2474, 2598, 3270, 3426
BstV2I GAAGAC 1 cut(s) 2571
BstX2I RGATCY 7 cut(s) 56, 238, 604, 874, 2167, 2444, 2557
BstXI CCANNNNNNTGG 1 cut(s) 540
BstYI RGATCY 7 cut(s) 56, 238, 604, 874, 2167, 2444, 2557
Bsu15I ATCGAT 1 cut(s) 577
Bsu36I CCTNAGG 1 cut(s) 2838
BsuI GTATCC 1 cut(s) 1129
BsuTUI ATCGAT 1 cut(s) 577
BtsI GCAGTG 2 cut(s) 3499, 3650
BtsIMutI CAGTG 6 cut(s) 1509, 2098, 2292, 2503, 3499, 3650
BveI ACCTGC 4 cut(s) 1059, 1335, 1611, 2467
Cac8I GCNNGC 6 cut(s) 2616, 2748, 2829, 3326, 3543, 3621
CaiI CAGNNNCTG 3 cut(s) 614, 1088, 1364
CfoI GCGC 2 cut(s) 141, 2302
Cfr10I RCCGGY 1 cut(s) 1593
Cfr13I GGNCC 2 cut(s) 1044, 2037
ClaI ATCGAT 1 cut(s) 577
Csp6I GTAC 4 cut(s) 357, 995, 1394, 2763
CviQI GTAC 4 cut(s) 357, 995, 1394, 2763
DraI TTTAAA 1 cut(s) 688
EaeI YGGCCR 1 cut(s) 3118
Eam1104I CTCTTC 4 cut(s) 404, 580, 979, 2817
EarI CTCTTC 4 cut(s) 404, 580, 979, 2817
Ecl136II GAGCTC 2 cut(s) 817, 2353
Eco130I CCWWGG 2 cut(s) 2528, 2631
Eco24I GRGCYC 4 cut(s) 819, 991, 1902, 2355
Eco31I GGTCTC 2 cut(s) 582, 2449
Eco32I GATATC 1 cut(s) 389
Eco47I GGWCC 2 cut(s) 1044, 2037
Eco53kI GAGCTC 2 cut(s) 817, 2353
Eco57I CTGAAG 4 cut(s) 737, 2148, 3138, 3294
Eco81I CCTNAGG 1 cut(s) 2838
Eco88I CYCGRG 5 cut(s) 897, 1000, 1276, 1552, 1993
EcoICRI GAGCTC 2 cut(s) 817, 2353
EcoRI GAATTC 5 cut(s) 2387, 3136, 3292, 3299, 3526
EcoRII CCWGG 5 cut(s) 8, 535, 877, 1542, 3388
EcoRV GATATC 1 cut(s) 389
EcoT14I CCWWGG 2 cut(s) 2528, 2631
EcoT22I ATGCAT 2 cut(s) 455, 685
EcoT38I GRGCYC 4 cut(s) 819, 991, 1902, 2355
ErhI CCWWGG 2 cut(s) 2528, 2631
Esp3I CGTCTC 1 cut(s) 2786
FalI AAGNNNNNCTT 2 cut(s) 3000, 3032
FaqI GGGAC 1 cut(s) 203
FauI CCCGC 6 cut(s) 39, 1778, 3254, 3410, 3550, 3566
FauNDI CATATG 1 cut(s) 2875
FblI GTMKAC 1 cut(s) 1548
Fnu4HI GCNGC 8 cut(s) 442, 2192, 2202, 2463, 2474, 2612, 3259, 3415
FriOI GRGCYC 4 cut(s) 819, 991, 1902, 2355
Fsp4HI GCNGC 8 cut(s) 442, 2192, 2202, 2463, 2474, 2612, 3259, 3415
FspBI CTAG 9 cut(s) 992, 1062, 1106, 1382, 2118, 2127, 3210, 3366, 3685
GlaI GCGC 2 cut(s) 140, 2301
GluI GCNGC 8 cut(s) 442, 2192, 2202, 2463, 2474, 2612, 3259, 3415
GsaI CCCAGC 6 cut(s) 1341, 1434, 1617, 2058, 2598, 3233
GsuI CTGGAG 3 cut(s) 684, 1212, 3372
HapII CCGG 7 cut(s) 20, 36, 348, 1594, 1634, 2690, 3624
HhaI GCGC 2 cut(s) 141, 2302
Hin1I GRCGYC 1 cut(s) 2795
Hin6I GCGC 2 cut(s) 139, 2300
HinP1I GCGC 2 cut(s) 139, 2300
HindIII AAGCTT 4 cut(s) 1577, 2744, 3061, 3628
HpaII CCGG 7 cut(s) 20, 36, 348, 1594, 1634, 2690, 3624
HphI GGTGA 5 cut(s) 179, 202, 869, 2752, 3048
Hpy166II GTNNAC 5 cut(s) 193, 997, 1549, 2096, 2176
Hpy8I GTNNAC 5 cut(s) 193, 997, 1549, 2096, 2176
Hpy99I CGWCG 3 cut(s) 1044, 1320, 2037
HpyCH4IV ACGT 6 cut(s) 810, 1971, 2151, 2627, 2795, 3396
HpySE526I ACGT 6 cut(s) 810, 1971, 2151, 2627, 2795, 3396
Hsp92I GRCGYC 1 cut(s) 2795
HspAI GCGC 2 cut(s) 139, 2300
Kpn2I TCCGGA 1 cut(s) 347
KpnI GGTACC 1 cut(s) 1397
LguI GCTCTTC 2 cut(s) 979, 2817
LmnI GCTCC 9 cut(s) 33, 283, 635, 1065, 1341, 1376, 1617, 1987, 2116
Lsp1109I GCAGC 8 cut(s) 453, 2188, 2203, 2460, 2474, 2598, 3270, 3426
LweI GCATC 7 cut(s) 121, 440, 2192, 2213, 2259, 2320, 2720
MaeI CTAG 9 cut(s) 992, 1062, 1106, 1382, 2118, 2127, 3210, 3366, 3685
MaeII ACGT 6 cut(s) 810, 1971, 2151, 2627, 2795, 3396
MaeIII GTNAC 6 cut(s) 332, 472, 559, 781, 1101, 2999
MbiI CCGCTC 1 cut(s) 630
MfeI CAATTG 2 cut(s) 1152, 2274
MflI RGATCY 7 cut(s) 56, 238, 604, 874, 2167, 2444, 2557
MhlI GDGCHC 6 cut(s) 777, 819, 991, 1902, 2355, 3560
MlsI TGGCCA 1 cut(s) 3120
MluNI TGGCCA 1 cut(s) 3120
MlyI GAGTC 6 cut(s) 605, 910, 1268, 1544, 1903, 2950
MmeI TCCRAC 7 cut(s) 254, 266, 295, 1329, 1465, 1605, 2138
Mox20I TGGCCA 1 cut(s) 3120
Mph1103I ATGCAT 2 cut(s) 455, 685
MroI TCCGGA 1 cut(s) 347
MroXI GAANNNNTTC 3 cut(s) 113, 3190, 3346
MscI TGGCCA 1 cut(s) 3120
MslI CAYNNNNRTG 4 cut(s) 479, 538, 1238, 1514
Msp20I TGGCCA 1 cut(s) 3120
MspA1I CMGCKG 7 cut(s) 1430, 1706, 2465, 3261, 3417, 3545, 3573
MspCI CTTAAG 1 cut(s) 2561
MspI CCGG 7 cut(s) 20, 36, 348, 1594, 1634, 2690, 3624
MspR9I CCNGG 7 cut(s) 10, 36, 537, 879, 1544, 2690, 3390
MunI CAATTG 2 cut(s) 1152, 2274
Mva1269I GAATGC 5 cut(s) 2393, 2948, 3143, 3168, 3220
MvaI CCWGG 5 cut(s) 10, 537, 879, 1544, 3390
NciI CCSGG 2 cut(s) 36, 2690
NdeI CATATG 1 cut(s) 2875
NlaIV GGNNCC 5 cut(s) 29, 240, 1395, 1671, 2292
NmuCI GTSAC 2 cut(s) 472, 781
NsiI ATGCAT 2 cut(s) 455, 685
NspI RCATGY 3 cut(s) 2831, 3328, 3406
NspV TTCGAA 1 cut(s) 416
OliI CACNNNNGTG 1 cut(s) 479
PaeI GCATGC 2 cut(s) 2831, 3328
PaeR7I CTCGAG 3 cut(s) 897, 1276, 1993
PciSI GCTCTTC 2 cut(s) 979, 2817
PcsI WCGNNNNNNNCGW 1 cut(s) 1007
PctI GAATGC 5 cut(s) 2393, 2948, 3143, 3168, 3220
PdmI GAANNNNTTC 3 cut(s) 113, 3190, 3346
PfeI GAWTC 4 cut(s) 2884, 2918, 3370, 3448
PflMI CCANNNNNTGG 1 cut(s) 614
PfoI TCCNGGA 1 cut(s) 3388
PkrI GCNGC 8 cut(s) 443, 2193, 2203, 2464, 2475, 2613, 3260, 3416
PleI GAGTC 6 cut(s) 605, 910, 1268, 1544, 1903, 2950
PpsI GAGTC 6 cut(s) 605, 910, 1268, 1544, 1903, 2950
Psp124BI GAGCTC 2 cut(s) 819, 2355
Psp1406I AACGTT 1 cut(s) 810
Psp6I CCWGG 5 cut(s) 8, 535, 877, 1542, 3388
PspFI CCCAGC 6 cut(s) 1337, 1430, 1613, 2054, 2594, 3229
PspGI CCWGG 5 cut(s) 8, 535, 877, 1542, 3388
PspN4I GGNNCC 5 cut(s) 29, 240, 1395, 1671, 2292
PspPI GGNCC 2 cut(s) 1044, 2037
PspXI VCTCGAGB 2 cut(s) 1276, 1993
PstI CTGCAG 2 cut(s) 2464, 2519
PstNI CAGNNNCTG 3 cut(s) 614, 1088, 1364
PsuI RGATCY 7 cut(s) 56, 238, 604, 874, 2167, 2444, 2557
PvuII CAGCTG 3 cut(s) 1430, 1706, 2465
RsaI GTAC 4 cut(s) 358, 996, 1395, 2764
RsaNI GTAC 4 cut(s) 357, 995, 1394, 2763
RseI CAYNNNNRTG 4 cut(s) 479, 538, 1238, 1514
SacI GAGCTC 2 cut(s) 819, 2355
SapI GCTCTTC 2 cut(s) 979, 2817
SatI GCNGC 8 cut(s) 442, 2192, 2202, 2463, 2474, 2612, 3259, 3415
Sau96I GGNCC 2 cut(s) 1044, 2037
SchI GAGTC 6 cut(s) 605, 910, 1268, 1544, 1903, 2950
ScrFI CCNGG 7 cut(s) 10, 36, 537, 879, 1544, 2690, 3390
SduI GDGCHC 6 cut(s) 777, 819, 991, 1902, 2355, 3560
SfaNI GCATC 7 cut(s) 121, 440, 2192, 2213, 2259, 2320, 2720
SfcI CTRYAG 3 cut(s) 2460, 2515, 3465
Sfr274I CTCGAG 3 cut(s) 897, 1276, 1993
SfuI TTCGAA 1 cut(s) 416
SinI GGWCC 2 cut(s) 1044, 2037
SlaI CTCGAG 3 cut(s) 897, 1276, 1993
SmiMI CAYNNNNRTG 4 cut(s) 479, 538, 1238, 1514
SmlI CTYRAG 7 cut(s) 770, 897, 1276, 1993, 2561, 2723, 3475
SmoI CTYRAG 7 cut(s) 770, 897, 1276, 1993, 2561, 2723, 3475
SphI GCATGC 2 cut(s) 2831, 3328
SspMI CTAG 9 cut(s) 992, 1062, 1106, 1382, 2118, 2127, 3210, 3366, 3685
SstI GAGCTC 2 cut(s) 819, 2355
StyD4I CCNGG 7 cut(s) 8, 34, 535, 877, 1542, 2688, 3388
StyI CCWWGG 2 cut(s) 2528, 2631
TaiI ACGT 6 cut(s) 813, 1974, 2154, 2630, 2798, 3399
TaqII GACCGA 2 cut(s) 1032, 2025
TatI WGTACW 3 cut(s) 356, 994, 2762
TfiI GAWTC 4 cut(s) 2884, 2918, 3370, 3448
TscAI CASTG 6 cut(s) 1516, 2098, 2292, 2503, 3499, 3650
TseFI GTSAC 2 cut(s) 472, 781
TseI GCWGC 8 cut(s) 441, 2191, 2201, 2462, 2473, 2611, 3258, 3414
Tsp45I GTSAC 2 cut(s) 472, 781
TspGWI ACGGA 4 cut(s) 260, 421, 473, 2930
TspRI CASTG 6 cut(s) 1516, 2098, 2292, 2503, 3499, 3650
Van91I CCANNNNNTGG 1 cut(s) 614
Vha464I CTTAAG 1 cut(s) 2561
VpaK11BI GGWCC 2 cut(s) 1044, 2037
XceI RCATGY 3 cut(s) 2831, 3328, 3406
XcmI CCANNNNNNNNNTGG 2 cut(s) 101, 2689
XhoI CTCGAG 3 cut(s) 897, 1276, 1993
XmiI GTMKAC 1 cut(s) 1548
XmnI GAANNNNTTC 3 cut(s) 113, 3190, 3346
XspI CTAG 9 cut(s) 992, 1062, 1106, 1382, 2118, 2127, 3210, 3366, 3685
ZraI GACGTC 1 cut(s) 2796
Zsp2I ATGCAT 2 cut(s) 455, 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.