Rh4BG123200

Methyl-CpG binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002094
Physical Location & Seq
Forward (+)
20646490 .. 20651702
5213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG123200.1

Sequence Viewer

Length: 471 bp
ATGGCCAACAACCATTCTCCTGCTAACTACAACAAAATAGAGATGACACGAGAGCCGTCCTTAGGGATCAAACTTTTTGGAACCTTCGTGTATCCCCCATTGTACTTGGCAACCCCATTTTCAAAAGTTGTAGCGGAACCAAATGAATCTGCCATTGCAAACGATCATTCCAACCCTCAACATGGTAGTCAAATAAAGTGCGGACCAGGGAGACCTAGAAAATCTGACAAGCTTAGCTACAGTAGTGGCAATACATCTAATTCTGGGAGGGGGAGAAGAATCAATAAGGGTAGGCCAGACTGGTTGCCACAAGGTTGGAAGATCATAGAAAGAATTCGCAAGAGTGGTAGAGATATCAATAAATTTTACAAGACTTATATTAGCCCGAAAGGACGCCACTTTTGGTCAAAGCCTCAAGTTTTGAAGCATCTCCAAAAAATACCAGTTGAAGTTGTGGTTGAACAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBD

Protein Analysis

156

Amino Acids

17.71

Weight (kDa)

10.2

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBD PF01429 99 - 146 4.4e-10 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 134, 201
AclWI GGATC 1 cut(s) 74
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 333, 362
AcyI GRCGYC 1 cut(s) 394
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 2 cut(s) 62, 182
AgsI TTSAA 4 cut(s) 123, 424, 449, 461
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 3 cut(s) 232, 237, 466
AluI AGCT 3 cut(s) 232, 237, 466
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 74
AoxI GGCC 2 cut(s) 3, 293
ApoI RAATTY 2 cut(s) 333, 362
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
Asp700I GAANNNNTTC 1 cut(s) 333
AspS9I GGNCC 1 cut(s) 203
AvaII GGWCC 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 61
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 48
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 1 cut(s) 216
BfmI CTRYAG 1 cut(s) 238
BfuI GTATCC 1 cut(s) 102
BlpI GCTNAGC 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 82, 138
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 436
Bpu1102I GCTNAGC 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 394
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 1 cut(s) 206
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 182
Bse1I ACTGG 2 cut(s) 305, 443
Bse21I CCTNAGG 1 cut(s) 61
Bse3DI GCAATG 1 cut(s) 153
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 206
BseLI CCNNNNNNNGG 2 cut(s) 62, 182
BseMI GCAATG 1 cut(s) 153
BseNI ACTGG 2 cut(s) 305, 443
BshFI GGCC 2 cut(s) 5, 295
BslI CCNNNNNNNGG 2 cut(s) 62, 182
BsmAI GTCTC 1 cut(s) 205
BsnI GGCC 2 cut(s) 5, 295
Bso31I GGTCTC 1 cut(s) 205
Bsp143I GATC 3 cut(s) 66, 163, 321
Bsp1720I GCTNAGC 1 cut(s) 233
BspACI CCGC 2 cut(s) 134, 201
BspANI GGCC 2 cut(s) 5, 295
BspLI GGNNCC 2 cut(s) 82, 138
BspPI GGATC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 205
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 2 cut(s) 305, 443
BssECI CCNNGG 1 cut(s) 206
BssMI GATC 3 cut(s) 66, 163, 321
BssNI GRCGYC 1 cut(s) 394
BssSI CACGAG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 242
BstACI GRCGYC 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 61, 233
BstKTI GATC 3 cut(s) 69, 166, 324
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 3 cut(s) 66, 163, 321
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 1 cut(s) 238
BstXI CCANNNNNNTGG 1 cut(s) 315
Bsu36I CCTNAGG 1 cut(s) 61
BsuI GTATCC 1 cut(s) 102
BsuRI GGCC 2 cut(s) 5, 295
Cfr13I GGNCC 1 cut(s) 203
CseI GACGC 1 cut(s) 402
Csp6I GTAC 1 cut(s) 103
CviAII CATG 1 cut(s) 182
CviJI RGCY 8 cut(s) 5, 55, 232, 237, 295, 384, 412, 466
CviKI_1 RGCY 8 cut(s) 5, 55, 232, 237, 295, 384, 412, 466
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 2 cut(s) 61, 233
DpnI GATC 3 cut(s) 68, 165, 323
DpnII GATC 3 cut(s) 66, 163, 321
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 205
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 1 cut(s) 203
Eco81I CCTNAGG 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 205
EcoRV GATATC 1 cut(s) 355
FaeI CATG 1 cut(s) 185
FaiI YATR 3 cut(s) 183, 326, 378
FatI CATG 1 cut(s) 181
FspBI CTAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 5, 295
HgaI GACGC 1 cut(s) 402
Hin1I GRCGYC 1 cut(s) 394
Hin1II CATG 1 cut(s) 185
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 2 cut(s) 146, 279
Hpy188I TCNGA 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4V TGCA 1 cut(s) 158
HpyF3I CTNAG 2 cut(s) 61, 233
Hsp92I GRCGYC 1 cut(s) 394
Hsp92II CATG 1 cut(s) 185
Kzo9I GATC 3 cut(s) 66, 163, 321
LpnPI CCDG 7 cut(s) 33, 192, 219, 249, 286, 309, 456
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 216
MalI GATC 3 cut(s) 68, 165, 323
MboI GATC 3 cut(s) 66, 163, 321
MboII GAAGA 2 cut(s) 288, 331
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 259, 333, 362
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 195, 296
MnlI CCTC 3 cut(s) 186, 261, 423
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 333
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 469
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 207
NdeII GATC 3 cut(s) 66, 163, 321
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 2 cut(s) 82, 138
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 146, 279
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 82, 138
PspPI GGNCC 1 cut(s) 203
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
SaqAI TTAA 1 cut(s) 469
Sau3AI GATC 3 cut(s) 66, 163, 321
Sau96I GGNCC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 207
SetI ASST 6 cut(s) 86, 217, 234, 239, 316, 468
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 238
SinI GGWCC 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 414
SmoI CTYRAG 1 cut(s) 414
Sse9I AATT 3 cut(s) 259, 333, 362
SsiI CCGC 2 cut(s) 134, 201
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 242
TasI AATT 3 cut(s) 259, 333, 362
TatI WGTACW 1 cut(s) 102
TfiI GAWTC 2 cut(s) 146, 279
Tru1I TTAA 1 cut(s) 469
Tru9I TTAA 1 cut(s) 469
TspDTI ATGAA 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 203
XapI RAATTY 2 cut(s) 333, 362
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.