FvH4_6g02180

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002815
Physical Location & Seq
Forward (+)
1301266 .. 1304196
2931 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02180.t1

Sequence Viewer

Length: 933 bp
ATGAGAAGAGGAGGTGGAGGAGGACATTCTTCTGGGAATTACAGGAACCCATGTCTGACTATGCACCAGCCATGGGCGTCTCTGCTCGTTTATGGCATCAAACGCATCGAGGGCAGGTCATGGCCTGCTCCCATTAGAGGACGTCTTTGGATTCATGCTGCTGGTAAGGTTCCAGAGGAAGCTACAATCAAAGCAATGGAGGCCTTCTACCGGGAAATTTATGCGGTGGATGGAATCACTGATATCAAGTTTCCCGAACACTATCCAATTTCAAGACTTTTAGGTTGTGTCGAAGTAGTTGGATGTGTTAGACGCGAAGAGTTAGCTGGTTGGGAGATGGTACCTCAAGGGGTGAGGCTAGAAGCACAAACCGATATGTGTTGGCTTTGTGAGCAGCCACAGAAACTGCTAATTCCGTTTGAAATGCGTGGCTACCAAGGTGTCTATAACTTGGAAAAGAAGATATATGAAGGTGCCATTAGAGGTCTTACACCAGTTGAAGTTCCTCTCCCTGTCAAATTTCCACTTCCAAATCCACAAGATCCATTTTCCTTGAAGCCGGGGTCTATATCTGCAAATGTCGATCAGTCTAGAATATCTGAAGTGGAAAGATCTTCGAGTCTCAATGCAGCCATAGCTGGTGCACAAGCAGCTGCGACACAGTTTTCCAAAAAAGCTCAACCTTTACCAACAACCTCCCTAAATGATACAGCCAAATCTGTGACAGTTCCAATCAAGAGCGAATCTTTTGAAGTGAGTAAAGCAATGCCCACTCATTCCAGTGAGAGCTCAAATGAGGGGTCTAGGACATCAAATGACAAAAATCAAATCAGAAGCCGTGATCAACCTGCTTCTGGATCTTTTAGACCTCATCCTGGTGCACCTTCTAAGAATGTTTATGAATGGAGGAGGCGAGGAGATGCCCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

311

Amino Acids

34.31

Weight (kDa)

9.15

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 19 - 104 5e-08 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 145
Acc36I ACCTGC 2 cut(s) 105, 856
Acc65I GGTACC 1 cut(s) 340
AccB1I GGYRCC 2 cut(s) 340, 473
AccII CGCG 1 cut(s) 315
AciI CCGC 1 cut(s) 224
AclWI GGATC 2 cut(s) 536, 865
AcsI RAATTY 2 cut(s) 216, 518
AcuI CTGAAG 1 cut(s) 621
AcyI GRCGYC 2 cut(s) 77, 142
AfaI GTAC 1 cut(s) 342
AfiI CCNNNNNNNGG 5 cut(s) 73, 137, 210, 854, 875
AgsI TTSAA 5 cut(s) 273, 422, 500, 556, 752
AjnI CCWGG 1 cut(s) 874
AluBI AGCT 6 cut(s) 182, 326, 638, 653, 677, 789
AluI AGCT 6 cut(s) 182, 326, 638, 653, 677, 789
Alw21I GWGCWC 3 cut(s) 646, 791, 883
Alw26I GTCTC 2 cut(s) 84, 626
Alw44I GTGCAC 2 cut(s) 642, 879
AlwI GGATC 2 cut(s) 536, 865
AlwNI CAGNNNCTG 1 cut(s) 406
AoxI GGCC 2 cut(s) 122, 201
ApaLI GTGCAC 2 cut(s) 642, 879
ApeKI GCWGC 5 cut(s) 158, 394, 629, 650, 653
ApoI RAATTY 2 cut(s) 216, 518
ArsI GACNNNNNNTTYG 2 cut(s) 785, 817
Asp718I GGTACC 1 cut(s) 340
AsuC2I CCSGG 2 cut(s) 212, 561
AsuHPI GGTGA 1 cut(s) 364
BaeGI GKGCMC 2 cut(s) 646, 883
BanI GGYRCC 2 cut(s) 340, 473
BanII GRGCYC 1 cut(s) 791
Bbv12I GWGCWC 3 cut(s) 646, 791, 883
BbvI GCAGC 5 cut(s) 145, 406, 640, 641, 662
BccI CCATC 2 cut(s) 224, 331
BceAI ACGGC 1 cut(s) 822
BciT130I CCWGG 1 cut(s) 876
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 2 cut(s) 212, 561
BcoDI GTCTC 2 cut(s) 84, 626
BfaI CTAG 3 cut(s) 359, 591, 804
BfuAI ACCTGC 2 cut(s) 105, 856
BglII AGATCT 1 cut(s) 611
BisI GCNGC 5 cut(s) 159, 395, 630, 651, 654
BlsI GCNGC 5 cut(s) 160, 396, 631, 652, 655
Bme1390I CCNGG 3 cut(s) 212, 561, 876
BmiI GGNNCC 4 cut(s) 47, 171, 342, 475
BmrFI CCNGG 3 cut(s) 212, 561, 876
BmrI ACTGGG 1 cut(s) 919
BmsI GCATC 3 cut(s) 105, 114, 910
BmuI ACTGGG 1 cut(s) 919
BpuEI CTTGAG 1 cut(s) 330
BpuMI CCSGG 2 cut(s) 212, 561
BsaHI GRCGYC 2 cut(s) 77, 142
BsaJI CCNNGG 3 cut(s) 71, 436, 560
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 137, 210, 854, 875
Bse1I ACTGG 3 cut(s) 494, 780, 925
Bse3DI GCAATG 2 cut(s) 201, 771
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 3 cut(s) 71, 436, 560
BseGI GGATG 3 cut(s) 235, 308, 871
BseLI CCNNNNNNNGG 5 cut(s) 73, 137, 210, 854, 875
BseMI GCAATG 2 cut(s) 201, 771
BseNI ACTGG 3 cut(s) 494, 780, 925
BseRI GAGGAG 4 cut(s) 24, 33, 922, 930
BseSI GKGCMC 2 cut(s) 646, 883
BseXI GCAGC 5 cut(s) 145, 406, 640, 641, 662
Bsh1236I CGCG 1 cut(s) 315
BshFI GGCC 2 cut(s) 124, 203
BshNI GGYRCC 2 cut(s) 340, 473
BsiHKAI GWGCWC 3 cut(s) 646, 791, 883
BsiSI CCGG 2 cut(s) 211, 560
BslI CCNNNNNNNGG 5 cut(s) 73, 137, 210, 854, 875
BsmAI GTCTC 2 cut(s) 84, 626
BsmBI CGTCTC 1 cut(s) 84
BsnI GGCC 2 cut(s) 124, 203
Bsp1286I GDGCHC 3 cut(s) 646, 791, 883
Bsp143I GATC 5 cut(s) 541, 583, 611, 841, 857
Bsp19I CCATGG 1 cut(s) 71
BspACI CCGC 1 cut(s) 224
BspANI GGCC 2 cut(s) 124, 203
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 4 cut(s) 47, 171, 342, 475
BspMI ACCTGC 2 cut(s) 105, 856
BspPI GGATC 2 cut(s) 536, 865
BspT107I GGYRCC 2 cut(s) 340, 473
BsrDI GCAATG 2 cut(s) 201, 771
BsrI ACTGG 3 cut(s) 494, 780, 925
BssECI CCNNGG 3 cut(s) 71, 436, 560
BssMI GATC 5 cut(s) 541, 583, 611, 841, 857
BssNI GRCGYC 2 cut(s) 77, 142
BssT1I CCWWGG 2 cut(s) 71, 436
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 2 cut(s) 663, 727
Bst6I CTCTTC 1 cut(s) 312
BstACI GRCGYC 2 cut(s) 77, 142
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 888
BstDSI CCRYGG 1 cut(s) 71
BstF5I GGATG 3 cut(s) 235, 308, 871
BstFNI CGCG 1 cut(s) 315
BstKTI GATC 5 cut(s) 544, 586, 614, 844, 860
BstMAI GTCTC 2 cut(s) 84, 626
BstMBI GATC 5 cut(s) 541, 583, 611, 841, 857
BstMWI GCNNNNNNNGC 6 cut(s) 102, 111, 200, 391, 635, 650
BstNI CCWGG 1 cut(s) 876
BstSCI CCNGG 3 cut(s) 210, 559, 874
BstSLI GKGCMC 2 cut(s) 646, 883
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 5 cut(s) 145, 406, 640, 641, 662
BstX2I RGATCY 3 cut(s) 541, 611, 857
BstYI RGATCY 3 cut(s) 541, 611, 857
BsuRI GGCC 2 cut(s) 124, 203
BtgI CCRYGG 1 cut(s) 71
BtsCI GGATG 3 cut(s) 235, 308, 871
BtsIMutI CAGTG 2 cut(s) 237, 787
BveI ACCTGC 2 cut(s) 105, 856
Cac8I GCNNGC 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 406
CseI GACGC 2 cut(s) 66, 321
Csp6I GTAC 1 cut(s) 341
CviAII CATG 4 cut(s) 51, 72, 120, 155
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 1 cut(s) 888
DpnI GATC 5 cut(s) 543, 585, 613, 843, 859
DpnII GATC 5 cut(s) 541, 583, 611, 841, 857
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Ecl136II GAGCTC 1 cut(s) 789
Eco130I CCWWGG 2 cut(s) 71, 436
Eco147I AGGCCT 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 791
Eco32I GATATC 1 cut(s) 244
Eco53kI GAGCTC 1 cut(s) 789
Eco57I CTGAAG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 789
EcoRII CCWGG 1 cut(s) 874
EcoRV GATATC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 71, 436
EcoT38I GRGCYC 1 cut(s) 791
ErhI CCWWGG 2 cut(s) 71, 436
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 4 cut(s) 54, 75, 123, 158
FatI CATG 4 cut(s) 50, 71, 119, 154
FbaI TGATCA 1 cut(s) 841
Fnu4HI GCNGC 5 cut(s) 159, 395, 630, 651, 654
FokI GGATG 3 cut(s) 242, 315, 858
FriOI GRGCYC 1 cut(s) 791
Fsp4HI GCNGC 5 cut(s) 159, 395, 630, 651, 654
FspBI CTAG 3 cut(s) 359, 591, 804
GluI GCNGC 5 cut(s) 159, 395, 630, 651, 654
HaeIII GGCC 2 cut(s) 124, 203
HapII CCGG 2 cut(s) 211, 560
HgaI GACGC 2 cut(s) 66, 321
Hin1I GRCGYC 2 cut(s) 77, 142
Hin1II CATG 4 cut(s) 54, 75, 123, 158
HinfI GANTC 4 cut(s) 151, 234, 619, 743
HpaII CCGG 2 cut(s) 211, 560
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 2 cut(s) 644, 881
Hpy188I TCNGA 3 cut(s) 57, 601, 833
Hpy188III TCNNGA 6 cut(s) 173, 254, 273, 591, 736, 855
Hpy8I GTNNAC 2 cut(s) 644, 881
HpyAV CCTTC 3 cut(s) 214, 464, 894
HpyCH4III ACNGT 2 cut(s) 663, 727
HpyCH4IV ACGT 1 cut(s) 142
HpyCH4V TGCA 5 cut(s) 64, 575, 629, 644, 881
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 111, 200, 391, 635, 650
HpyF3I CTNAG 1 cut(s) 888
HpySE526I ACGT 1 cut(s) 142
Hsp92I GRCGYC 2 cut(s) 77, 142
Hsp92II CATG 4 cut(s) 54, 75, 123, 158
KpnI GGTACC 1 cut(s) 344
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 5 cut(s) 541, 583, 611, 841, 857
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 5 cut(s) 145, 406, 640, 641, 662
LweI GCATC 3 cut(s) 105, 114, 910
MaeI CTAG 3 cut(s) 359, 591, 804
MaeII ACGT 1 cut(s) 142
MaeIII GTNAC 1 cut(s) 721
MalI GATC 5 cut(s) 543, 585, 613, 843, 859
MboI GATC 5 cut(s) 541, 583, 611, 841, 857
MboII GAAGA 5 cut(s) 18, 21, 329, 472, 606
MflI RGATCY 3 cut(s) 541, 611, 857
MhlI GDGCHC 3 cut(s) 646, 791, 883
MluCI AATT 5 cut(s) 37, 216, 267, 411, 518
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 1 cut(s) 280
MslI CAYNNNNRTG 2 cut(s) 780, 876
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 2 cut(s) 211, 560
MspR9I CCNGG 3 cut(s) 212, 561, 876
MvaI CCWGG 1 cut(s) 876
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 6 cut(s) 102, 111, 200, 391, 635, 650
NciI CCSGG 2 cut(s) 212, 561
NcoI CCATGG 1 cut(s) 71
NdeII GATC 5 cut(s) 541, 583, 611, 841, 857
NlaIII CATG 4 cut(s) 54, 75, 123, 158
NlaIV GGNNCC 4 cut(s) 47, 171, 342, 475
NmuCI GTSAC 1 cut(s) 721
PceI AGGCCT 1 cut(s) 203
PfeI GAWTC 3 cut(s) 151, 234, 743
PkrI GCNGC 5 cut(s) 160, 396, 631, 652, 655
PleI GAGTC 1 cut(s) 627
PpsI GAGTC 1 cut(s) 627
Psp124BI GAGCTC 1 cut(s) 791
Psp6I CCWGG 1 cut(s) 874
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 4 cut(s) 47, 171, 342, 475
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 3 cut(s) 541, 611, 857
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
RseI CAYNNNNRTG 2 cut(s) 780, 876
SacI GAGCTC 1 cut(s) 791
SatI GCNGC 5 cut(s) 159, 395, 630, 651, 654
Sau3AI GATC 5 cut(s) 541, 583, 611, 841, 857
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 3 cut(s) 212, 561, 876
SduI GDGCHC 3 cut(s) 646, 791, 883
SfaNI GCATC 3 cut(s) 105, 114, 910
SmiMI CAYNNNNRTG 2 cut(s) 780, 876
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 5 cut(s) 37, 216, 267, 411, 518
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 1 cut(s) 224
SspMI CTAG 3 cut(s) 359, 591, 804
SstI GAGCTC 1 cut(s) 791
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 3 cut(s) 210, 559, 874
StyI CCWWGG 2 cut(s) 71, 436
TaaI ACNGT 2 cut(s) 663, 727
TaiI ACGT 1 cut(s) 145
TaqI TCGA 4 cut(s) 108, 291, 582, 617
TasI AATT 5 cut(s) 37, 216, 267, 411, 518
TfiI GAWTC 3 cut(s) 151, 234, 743
TscAI CASTG 2 cut(s) 244, 787
TseFI GTSAC 1 cut(s) 721
TseI GCWGC 5 cut(s) 158, 394, 629, 650, 653
Tsp45I GTSAC 1 cut(s) 721
TspDTI ATGAA 3 cut(s) 143, 483, 915
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 2 cut(s) 244, 787
VneI GTGCAC 2 cut(s) 642, 879
XapI RAATTY 2 cut(s) 216, 518
XbaI TCTAGA 1 cut(s) 590
XspI CTAG 3 cut(s) 359, 591, 804
ZraI GACGTC 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.