Rmu_sc0002646.1_g000003

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002646.1
OG0002815
Physical Location & Seq
Forward (+)
20010 .. 21634
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002646.1_g000003.1.cds

Sequence Viewer

Length: 1035 bp
atgagaagaggaggaggaggttcttcagggaattacagaaacccatgtctgactatgcaccagccatgggcttcactgctcgtttatggtatcaaacgcatcgagggcaggtcatggcctgcttccattagaggtttgggtcttgtaaactttggtttcaactcaaattcagatacccattttgtagtgttggaagaactagtagaacttattagtaaagctgcagatttgaatgtcttgtttttgattggtcaacttgctcaattgtctaccaaaggacgtctttggattcatgcagctggtaaggttccagaggagtctacaatcaaagcaatggaggatttctatcgggaaatatatgctgtggatggagtcactgatatcaaatttccccaacactatccagtttcaaaactgctagggtgtgttgaagtagttggatgtgttagacgtgaagagttagctggttgggagatggttcctgaaggggtgaggctggaagcacaaaccgatatgtgttggctttgtgagcagccacagaaactgttaattccgtttgagatgcgtggctaccaaggtgtctataacttggaaaagaagatatatgaagctgctattagaggtctcaccccggttgaagttcctctcccagttaaatttccacttccaaatccacaagatccattttccttgaagccagggtctatatctgcaaacgttgatcaatctagaacatctgaagcgggaagatcttcgagtctcaatgcagctatagctggtgcacgagcagctgctacacagttctccaaaaaacctcaaactttcccaactacctccctaaatgatgcagccaaatctgtgacagttccaatcaagagtgaatcttttgaaggaggaagagcaatgaccggtaatttcagtgagagctccaacaaagagtcaatgacatcaaataacaaagatcaaatcagcagccgtaatcaaccttcttctggagctattagacctcatcctggtgcaccttctaaggtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

344

Amino Acids

37.54

Weight (kDa)

8.71

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 283
Acc36I ACCTGC 1 cut(s) 99
AccI GTMKAC 2 cut(s) 269, 320
AciI CCGC 1 cut(s) 743
AclI AACGTT 1 cut(s) 717
AclWI GGATC 1 cut(s) 674
AcsI RAATTY 3 cut(s) 166, 386, 656
AcuI CTGAAG 3 cut(s) 9, 504, 759
AcyI GRCGYC 1 cut(s) 280
AfiI CCNNNNNNNGG 3 cut(s) 67, 992, 1013
AgeI ACCGGT 1 cut(s) 908
AgsI TTSAA 7 cut(s) 160, 232, 411, 431, 638, 694, 890
AhlI ACTAGT 1 cut(s) 199
AjiI CACGTC 1 cut(s) 452
AjnI CCWGG 2 cut(s) 697, 1012
AluBI AGCT 9 cut(s) 221, 299, 464, 611, 770, 776, 791, 927, 998
AluI AGCT 9 cut(s) 221, 299, 464, 611, 770, 776, 791, 927, 998
Alw21I GWGCWC 3 cut(s) 784, 929, 1021
Alw26I GTCTC 2 cut(s) 629, 764
Alw44I GTGCAC 2 cut(s) 780, 1017
AlwI GGATC 1 cut(s) 674
AlwNI CAGNNNCTG 1 cut(s) 544
AoxI GGCC 1 cut(s) 116
ApaLI GTGCAC 2 cut(s) 780, 1017
ApeKI GCWGC 9 cut(s) 221, 296, 532, 611, 767, 788, 791, 848, 972
ApoI RAATTY 3 cut(s) 166, 386, 656
AsiGI ACCGGT 1 cut(s) 908
Asp700I GAANNNNTTC 1 cut(s) 751
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 2 cut(s) 502, 619
BaeGI GKGCMC 2 cut(s) 784, 1021
BanII GRGCYC 1 cut(s) 929
BarI GAAGNNNNNNTAC 2 cut(s) 970, 1002
BauI CACGAG 1 cut(s) 783
Bbv12I GWGCWC 3 cut(s) 784, 929, 1021
BbvI GCAGC 9 cut(s) 208, 308, 544, 598, 778, 779, 800, 860, 984
BccI CCATC 2 cut(s) 362, 469
BceAI ACGGC 1 cut(s) 960
BciT130I CCWGG 2 cut(s) 699, 1014
BclI TGATCA 1 cut(s) 721
BcnI CCSGG 1 cut(s) 632
BcoDI GTCTC 2 cut(s) 629, 764
BcuI ACTAGT 1 cut(s) 199
BfaI CTAG 3 cut(s) 200, 419, 729
BfmI CTRYAG 2 cut(s) 222, 771
BfuAI ACCTGC 1 cut(s) 99
BglII AGATCT 1 cut(s) 749
BisI GCNGC 9 cut(s) 222, 297, 533, 612, 768, 789, 792, 849, 973
BlsI GCNGC 9 cut(s) 223, 298, 534, 613, 769, 790, 793, 850, 974
Bme1390I CCNGG 3 cut(s) 632, 699, 1014
BmgBI CACGTC 1 cut(s) 452
BmiI GGNNCC 2 cut(s) 309, 480
BmrFI CCNGG 3 cut(s) 632, 699, 1014
BmrI ACTGGG 1 cut(s) 644
BmsI GCATC 3 cut(s) 108, 552, 835
BmuI ACTGGG 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 1014
BpuMI CCSGG 1 cut(s) 632
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 4 cut(s) 65, 574, 630, 698
BsaWI WCCGGW 1 cut(s) 908
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 992, 1013
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 2 cut(s) 404, 650
Bse3DI GCAATG 2 cut(s) 339, 909
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 2 cut(s) 699, 1014
BseDI CCNNGG 4 cut(s) 65, 574, 630, 698
BseGI GGATG 3 cut(s) 373, 446, 1009
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 67, 992, 1013
BseMI GCAATG 2 cut(s) 339, 909
BseNI ACTGG 2 cut(s) 404, 650
BseRI GAGGAG 4 cut(s) 24, 27, 30, 329
BseSI GKGCMC 2 cut(s) 784, 1021
BseXI GCAGC 9 cut(s) 208, 308, 544, 598, 778, 779, 800, 860, 984
BshFI GGCC 1 cut(s) 118
BshTI ACCGGT 1 cut(s) 908
BsiHKAI GWGCWC 3 cut(s) 784, 929, 1021
BsiSI CCGG 2 cut(s) 632, 909
BslI CCNNNNNNNGG 3 cut(s) 67, 992, 1013
BsmAI GTCTC 2 cut(s) 629, 764
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 629
Bsp1286I GDGCHC 3 cut(s) 784, 929, 1021
Bsp143I GATC 4 cut(s) 679, 721, 749, 961
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 1 cut(s) 743
BspANI GGCC 1 cut(s) 118
BspLI GGNNCC 2 cut(s) 309, 480
BspMAI CTGCAG 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 1 cut(s) 674
BspQI GCTCTTC 1 cut(s) 892
BspTNI GGTCTC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 339, 909
BsrFI RCCGGY 1 cut(s) 908
BsrI ACTGG 2 cut(s) 404, 650
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 4 cut(s) 65, 574, 630, 698
BssMI GATC 4 cut(s) 679, 721, 749, 961
BssNI GRCGYC 1 cut(s) 280
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 2 cut(s) 65, 574
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 699, 1014
Bst4CI ACNGT 3 cut(s) 546, 801, 865
Bst6I CTCTTC 2 cut(s) 450, 892
BstACI GRCGYC 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 1026
BstDSI CCRYGG 1 cut(s) 65
BstF5I GGATG 3 cut(s) 373, 446, 1009
BstKTI GATC 4 cut(s) 682, 724, 752, 964
BstMAI GTCTC 2 cut(s) 629, 764
BstMBI GATC 4 cut(s) 679, 721, 749, 961
BstMWI GCNNNNNNNGC 4 cut(s) 105, 529, 773, 788
BstNI CCWGG 2 cut(s) 699, 1014
BstSCI CCNGG 3 cut(s) 630, 697, 1012
BstSFI CTRYAG 2 cut(s) 222, 771
BstSLI GKGCMC 2 cut(s) 784, 1021
BstV1I GCAGC 9 cut(s) 208, 308, 544, 598, 778, 779, 800, 860, 984
BstX2I RGATCY 2 cut(s) 679, 749
BstYI RGATCY 2 cut(s) 679, 749
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 65
BtrI CACGTC 1 cut(s) 452
BtsCI GGATG 3 cut(s) 373, 446, 1009
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 3 cut(s) 74, 375, 925
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 120
CaiI CAGNNNCTG 1 cut(s) 544
Cfr10I RCCGGY 1 cut(s) 908
CspAI ACCGGT 1 cut(s) 908
CviAII CATG 4 cut(s) 45, 66, 114, 293
DdeI CTNAG 1 cut(s) 1026
DpnI GATC 4 cut(s) 681, 723, 751, 963
DpnII GATC 4 cut(s) 679, 721, 749, 961
Eam1104I CTCTTC 2 cut(s) 450, 892
EarI CTCTTC 2 cut(s) 450, 892
Ecl136II GAGCTC 1 cut(s) 927
Eco130I CCWWGG 2 cut(s) 65, 574
Eco24I GRGCYC 1 cut(s) 929
Eco31I GGTCTC 1 cut(s) 629
Eco32I GATATC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 927
Eco57I CTGAAG 3 cut(s) 9, 504, 759
EcoICRI GAGCTC 1 cut(s) 927
EcoRII CCWGG 2 cut(s) 697, 1012
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 2 cut(s) 65, 574
EcoT38I GRGCYC 1 cut(s) 929
ErhI CCWWGG 2 cut(s) 65, 574
FaeI CATG 4 cut(s) 48, 69, 117, 296
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FatI CATG 4 cut(s) 44, 65, 113, 292
FauI CCCGC 1 cut(s) 736
FbaI TGATCA 1 cut(s) 721
FblI GTMKAC 2 cut(s) 269, 320
Fnu4HI GCNGC 9 cut(s) 222, 297, 533, 612, 768, 789, 792, 849, 973
FokI GGATG 3 cut(s) 380, 453, 996
FriOI GRGCYC 1 cut(s) 929
Fsp4HI GCNGC 9 cut(s) 222, 297, 533, 612, 768, 789, 792, 849, 973
FspBI CTAG 3 cut(s) 200, 419, 729
GluI GCNGC 9 cut(s) 222, 297, 533, 612, 768, 789, 792, 849, 973
GsuI CTGGAG 1 cut(s) 1014
HaeIII GGCC 1 cut(s) 118
HapII CCGG 2 cut(s) 632, 909
Hin1I GRCGYC 1 cut(s) 280
Hin1II CATG 4 cut(s) 48, 69, 117, 296
HincII GTYRAC 1 cut(s) 254
HindII GTYRAC 1 cut(s) 254
HinfI GANTC 6 cut(s) 289, 317, 372, 757, 881, 938
HpaII CCGG 2 cut(s) 632, 909
HphI GGTGA 2 cut(s) 502, 619
Hpy166II GTNNAC 6 cut(s) 148, 254, 270, 321, 782, 1019
Hpy188I TCNGA 3 cut(s) 51, 172, 739
Hpy188III TCNNGA 6 cut(s) 311, 350, 482, 729, 874, 993
Hpy8I GTNNAC 6 cut(s) 148, 254, 270, 321, 782, 1019
HpyAV CCTTC 4 cut(s) 479, 884, 996, 1032
HpyCH4III ACNGT 3 cut(s) 546, 801, 865
HpyCH4IV ACGT 3 cut(s) 280, 451, 717
HpyCH4V TGCA 8 cut(s) 58, 224, 296, 713, 767, 782, 848, 1019
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 529, 773, 788
HpyF3I CTNAG 1 cut(s) 1026
HpySE526I ACGT 3 cut(s) 280, 451, 717
Hsp92I GRCGYC 1 cut(s) 280
Hsp92II CATG 4 cut(s) 48, 69, 117, 296
Ksp22I TGATCA 1 cut(s) 721
Kzo9I GATC 4 cut(s) 679, 721, 749, 961
LguI GCTCTTC 1 cut(s) 892
LmnI GCTCC 2 cut(s) 932, 995
Lsp1109I GCAGC 9 cut(s) 208, 308, 544, 598, 778, 779, 800, 860, 984
LweI GCATC 3 cut(s) 108, 552, 835
MaeI CTAG 3 cut(s) 200, 419, 729
MaeII ACGT 3 cut(s) 280, 451, 717
MaeIII GTNAC 2 cut(s) 373, 859
MalI GATC 4 cut(s) 681, 723, 751, 963
MboI GATC 4 cut(s) 679, 721, 749, 961
MboII GAAGA 9 cut(s) 15, 18, 206, 467, 610, 744, 759, 909, 981
MfeI CAATTG 1 cut(s) 263
MflI RGATCY 2 cut(s) 679, 749
MhlI GDGCHC 3 cut(s) 784, 929, 1021
MluCI AATT 7 cut(s) 31, 166, 263, 386, 549, 656, 913
MlyI GAGTC 4 cut(s) 326, 381, 766, 947
MmeI TCCRAC 3 cut(s) 171, 418, 954
MroXI GAANNNNTTC 1 cut(s) 751
MseI TTAA 2 cut(s) 548, 654
MslI CAYNNNNRTG 1 cut(s) 1014
MspA1I CMGCKG 2 cut(s) 299, 791
MspI CCGG 2 cut(s) 632, 909
MspR9I CCNGG 3 cut(s) 632, 699, 1014
MunI CAATTG 1 cut(s) 263
MvaI CCWGG 2 cut(s) 699, 1014
MwoI GCNNNNNNNGC 4 cut(s) 105, 529, 773, 788
NciI CCSGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 65
NdeII GATC 4 cut(s) 679, 721, 749, 961
NlaIII CATG 4 cut(s) 48, 69, 117, 296
NlaIV GGNNCC 2 cut(s) 309, 480
NmuCI GTSAC 2 cut(s) 373, 859
PciSI GCTCTTC 1 cut(s) 892
PdmI GAANNNNTTC 1 cut(s) 751
PfeI GAWTC 2 cut(s) 289, 881
PinAI ACCGGT 1 cut(s) 908
PkrI GCNGC 9 cut(s) 223, 298, 534, 613, 769, 790, 793, 850, 974
PleI GAGTC 4 cut(s) 325, 380, 765, 946
PpsI GAGTC 4 cut(s) 325, 380, 765, 946
Psp124BI GAGCTC 1 cut(s) 929
Psp1406I AACGTT 1 cut(s) 717
Psp6I CCWGG 2 cut(s) 697, 1012
PspGI CCWGG 2 cut(s) 697, 1012
PspN4I GGNNCC 2 cut(s) 309, 480
PstI CTGCAG 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 544
PsuI RGATCY 2 cut(s) 679, 749
PvuII CAGCTG 2 cut(s) 299, 791
RseI CAYNNNNRTG 1 cut(s) 1014
SacI GAGCTC 1 cut(s) 929
SapI GCTCTTC 1 cut(s) 892
SaqAI TTAA 2 cut(s) 548, 654
SatI GCNGC 9 cut(s) 222, 297, 533, 612, 768, 789, 792, 849, 973
Sau3AI GATC 4 cut(s) 679, 721, 749, 961
SchI GAGTC 4 cut(s) 326, 381, 766, 947
ScrFI CCNGG 3 cut(s) 632, 699, 1014
SduI GDGCHC 3 cut(s) 784, 929, 1021
SfaNI GCATC 3 cut(s) 108, 552, 835
SfcI CTRYAG 2 cut(s) 222, 771
SmiMI CAYNNNNRTG 1 cut(s) 1014
SpeI ACTAGT 1 cut(s) 199
Sse9I AATT 7 cut(s) 31, 166, 263, 386, 549, 656, 913
SsiI CCGC 1 cut(s) 743
SspMI CTAG 3 cut(s) 200, 419, 729
SstI GAGCTC 1 cut(s) 929
StyD4I CCNGG 3 cut(s) 630, 697, 1012
StyI CCWWGG 2 cut(s) 65, 574
TaaI ACNGT 3 cut(s) 546, 801, 865
TaiI ACGT 3 cut(s) 283, 454, 720
TaqI TCGA 2 cut(s) 102, 755
TasI AATT 7 cut(s) 31, 166, 263, 386, 549, 656, 913
TfiI GAWTC 2 cut(s) 289, 881
Tru1I TTAA 2 cut(s) 548, 654
Tru9I TTAA 2 cut(s) 548, 654
TscAI CASTG 3 cut(s) 81, 382, 925
TseFI GTSAC 2 cut(s) 373, 859
TseI GCWGC 9 cut(s) 221, 296, 532, 611, 767, 788, 791, 848, 972
Tsp45I GTSAC 2 cut(s) 373, 859
TspDTI ATGAA 2 cut(s) 281, 621
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 3 cut(s) 81, 382, 925
VneI GTGCAC 2 cut(s) 780, 1017
XapI RAATTY 3 cut(s) 166, 386, 656
XbaI TCTAGA 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmiI GTMKAC 2 cut(s) 269, 320
XmnI GAANNNNTTC 1 cut(s) 751
XspI CTAG 3 cut(s) 200, 419, 729
ZraI GACGTC 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.