RchiOBHm_Chr3g0449951

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002815
Physical Location & Seq
Forward (+)
1603035 .. 1607225
4191 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41730

Sequence Viewer

Length: 1359 bp
ATGAGAAGAGGAGGAGGAGGTTCTTCAGGGAATTACAGAAACCCATGTCTGACTATGCACCAGCCATGGGCTTCCCTGCTCGTTTATGGTATCAAACGCATCGAGGGCAGGTCATGGCCTGCTCCCATTAGAGGACGTCTTTGGATTCATGCAGCTGGTAAGGTTCCAGAGGAGTCTACAATCAAAGCAATGGAGGATTTCTATCGGGAAATTTATGCTGTGGATGGAGTCACTGATATCAAATTTCCCCAACACTATCCAGTTTCAAGACTGCTAGGGTGTGTTGAAGTAGTTGGATGTGTTAGACGTGAAGAGTTAGCTGGTTGGGAGATGGTTCCTGAAGGGGTGAGGCTGGAAGCACAAACCGATATGTGTTGGCTTTGTGAGCAGCCACAGAAACTGTTAATTCCGTTTGAGATGCGTGGCTACCAAGGTGTCTATAACTTGGAAAAGAAGATATATGAAGCTGCTATTAGAGGTCTCACCCCGGTTGAAGTTCCTCTCCCAGTTAAATTTCCACTTCCAAATCCACAAGATCCATTTTCCTTGAAGCCAGGGTCTATATCTGCAAACGTTGATCAATCTAGAACATCTGAAGCGGGAAGATCTTCGAGTCTCAATGCAGCTATAGCTGGTGCACGAGCAGCTGCTACACAGTTCTCCAAAAAACCTCAAACTTTCCCAACTACCTCCCTAAATGATGCAGCCAAATCTGTGACAGTTCCAATCAAGAGTGAATCTTTTGAAGGAGGAAGAGCAATGACCGGTAATTTCAGTGAGAGCTCCAACAAAGAGTCAATGACATCAAATAACAAAGATCAAATCAGCAGCCGTAATCAACCTTCTTCTGGAGCTATTAGACCTCATCCTGGTGCACCTTCTAAGATATATGAAGCGGCTATTAGAGGTCTTACCCCTGTTGAAGTTCCCCTTAAAGTCAAATTTCCAGTTCCAAATCCAGAAGATCCATTTTCCTTGAAGCCAGGAGTTATATCTGCTAATGTCGATCAGTCTAGAACATCTGAAGTGGTAAGATCTTCTAGTCTCAATACAGCCATAGCTGGTGCACAAGCAGCTGATACACAGTTCTCCAAAAAAACTCAAACTTTCCCAACTACCTCCCTAAATAATGCCGCGGAATCTGTGATGACAGTTCCAATCAGGAGTGAGTCTTCTGAAGTGGGTAAAGTGACGTCTGCTAATTTTATTGAGAGTTCTAATAAGAGGTCTATGACATCAAATAACAAAGATCAAATCAGCAGAGGTGATCAACCTTCTTCTGGAGCTTTTAGACCTCATCCTGGTGCACCTTCTAAGTATGTTTATGAACGGAGGAGGCGACGAGATGCCCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

452

Amino Acids

49.38

Weight (kDa)

9.47

Isoelectric Point (pI)

57.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 17 - 105 3.9e-08 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 139, 1196
Acc36I ACCTGC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 176
AccII CGCG 1 cut(s) 1136
AciI CCGC 4 cut(s) 599, 896, 1134, 1136
AclI AACGTT 1 cut(s) 573
AclWI GGATC 2 cut(s) 530, 959
AcsI RAATTY 4 cut(s) 210, 242, 512, 941
AcuI CTGAAG 5 cut(s) 9, 360, 615, 1044, 1197
AcyI GRCGYC 2 cut(s) 136, 1193
AfiI CCNNNNNNNGG 6 cut(s) 67, 131, 848, 869, 1280, 1301
AgeI ACCGGT 1 cut(s) 764
AgsI TTSAA 7 cut(s) 267, 287, 494, 550, 746, 923, 979
AjiI CACGTC 1 cut(s) 308
AjnI CCWGG 4 cut(s) 553, 868, 982, 1300
Alw21I GWGCWC 5 cut(s) 640, 785, 877, 1069, 1309
Alw26I GTCTC 3 cut(s) 485, 620, 1049
Alw44I GTGCAC 4 cut(s) 636, 873, 1065, 1305
AlwI GGATC 2 cut(s) 530, 959
AlwNI CAGNNNCTG 1 cut(s) 400
AoxI GGCC 1 cut(s) 116
ApaLI GTGCAC 4 cut(s) 636, 873, 1065, 1305
ApeKI GCWGC 9 cut(s) 152, 388, 467, 623, 644, 647, 704, 828, 1073
ApoI RAATTY 4 cut(s) 210, 242, 512, 941
AsiGI ACCGGT 1 cut(s) 764
Asp700I GAANNNNTTC 1 cut(s) 607
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 3 cut(s) 358, 475, 1277
BaeGI GKGCMC 4 cut(s) 640, 877, 1069, 1309
BanII GRGCYC 1 cut(s) 785
BarI GAAGNNNNNNTAC 2 cut(s) 826, 858
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 1 cut(s) 1164
Bbv12I GWGCWC 5 cut(s) 640, 785, 877, 1069, 1309
BbvI GCAGC 9 cut(s) 164, 400, 454, 634, 635, 656, 716, 840, 1085
BccI CCATC 2 cut(s) 218, 325
BceAI ACGGC 1 cut(s) 816
BciT130I CCWGG 4 cut(s) 555, 870, 984, 1302
BclI TGATCA 2 cut(s) 577, 1267
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 3 cut(s) 485, 620, 1049
BfaI CTAG 4 cut(s) 275, 585, 1014, 1041
BfmI CTRYAG 1 cut(s) 627
BfuAI ACCTGC 1 cut(s) 99
BglII AGATCT 2 cut(s) 605, 1034
Bme1390I CCNGG 5 cut(s) 488, 555, 870, 984, 1302
BmgBI CACGTC 1 cut(s) 308
BmiI GGNNCC 2 cut(s) 165, 336
BmrFI CCNGG 5 cut(s) 488, 555, 870, 984, 1302
BmrI ACTGGG 2 cut(s) 500, 1345
BmsI GCATC 4 cut(s) 108, 408, 691, 1336
BmuI ACTGGG 2 cut(s) 500, 1345
BpiI GAAGAC 1 cut(s) 1164
BpmI CTGGAG 2 cut(s) 870, 1302
BpuMI CCSGG 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 201
BsaHI GRCGYC 2 cut(s) 136, 1193
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 5 cut(s) 65, 430, 486, 554, 1134
BsaWI WCCGGW 1 cut(s) 764
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 131, 848, 869, 1280, 1301
Bse118I RCCGGY 1 cut(s) 764
Bse1I ACTGG 4 cut(s) 260, 506, 947, 1351
Bse3DI GCAATG 2 cut(s) 195, 765
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 4 cut(s) 555, 870, 984, 1302
BseDI CCNNGG 5 cut(s) 65, 430, 486, 554, 1134
BseGI GGATG 4 cut(s) 229, 302, 865, 1297
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 6 cut(s) 67, 131, 848, 869, 1280, 1301
BseMI GCAATG 2 cut(s) 195, 765
BseNI ACTGG 4 cut(s) 260, 506, 947, 1351
BseRI GAGGAG 5 cut(s) 24, 27, 30, 185, 1348
BseSI GKGCMC 4 cut(s) 640, 877, 1069, 1309
BseXI GCAGC 9 cut(s) 164, 400, 454, 634, 635, 656, 716, 840, 1085
Bsh1236I CGCG 1 cut(s) 1136
BshFI GGCC 1 cut(s) 118
BshTI ACCGGT 1 cut(s) 764
BsiHKAI GWGCWC 5 cut(s) 640, 785, 877, 1069, 1309
BsiSI CCGG 2 cut(s) 488, 765
BslI CCNNNNNNNGG 6 cut(s) 67, 131, 848, 869, 1280, 1301
BsmAI GTCTC 3 cut(s) 485, 620, 1049
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 485
Bsp1286I GDGCHC 5 cut(s) 640, 785, 877, 1069, 1309
Bsp143I GATC 9 cut(s) 535, 577, 605, 817, 964, 1006, 1034, 1249, 1267
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 4 cut(s) 599, 896, 1134, 1136
BspANI GGCC 1 cut(s) 118
BspFNI CGCG 1 cut(s) 1136
BspLI GGNNCC 2 cut(s) 165, 336
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 2 cut(s) 530, 959
BspQI GCTCTTC 1 cut(s) 748
BspTNI GGTCTC 1 cut(s) 485
BsrDI GCAATG 2 cut(s) 195, 765
BsrFI RCCGGY 1 cut(s) 764
BsrI ACTGG 4 cut(s) 260, 506, 947, 1351
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 5 cut(s) 65, 430, 486, 554, 1134
BssMI GATC 9 cut(s) 535, 577, 605, 817, 964, 1006, 1034, 1249, 1267
BssNI GRCGYC 2 cut(s) 136, 1193
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 2 cut(s) 65, 430
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 4 cut(s) 555, 870, 984, 1302
Bst4CI ACNGT 5 cut(s) 402, 657, 721, 1086, 1153
Bst6I CTCTTC 2 cut(s) 306, 748
BstACI GRCGYC 2 cut(s) 136, 1193
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 882, 1314
BstDSI CCRYGG 2 cut(s) 65, 1134
BstF5I GGATG 4 cut(s) 229, 302, 865, 1297
BstFNI CGCG 1 cut(s) 1136
BstKTI GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
BstMAI GTCTC 3 cut(s) 485, 620, 1049
BstMBI GATC 9 cut(s) 535, 577, 605, 817, 964, 1006, 1034, 1249, 1267
BstMWI GCNNNNNNNGC 5 cut(s) 105, 385, 629, 644, 1073
BstNI CCWGG 4 cut(s) 555, 870, 984, 1302
BstSCI CCNGG 5 cut(s) 486, 553, 868, 982, 1300
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 4 cut(s) 640, 877, 1069, 1309
BstUI CGCG 1 cut(s) 1136
BstV1I GCAGC 9 cut(s) 164, 400, 454, 634, 635, 656, 716, 840, 1085
BstV2I GAAGAC 1 cut(s) 1164
BstX2I RGATCY 4 cut(s) 535, 605, 964, 1034
BstYI RGATCY 4 cut(s) 535, 605, 964, 1034
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 2 cut(s) 65, 1134
BtrI CACGTC 1 cut(s) 308
BtsCI GGATG 4 cut(s) 229, 302, 865, 1297
BtsIMutI CAGTG 2 cut(s) 231, 781
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 120
CaiI CAGNNNCTG 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 764
Cfr42I CCGCGG 1 cut(s) 1137
CspAI ACCGGT 1 cut(s) 764
CviAII CATG 4 cut(s) 45, 66, 114, 149
DdeI CTNAG 2 cut(s) 882, 1314
DpnI GATC 9 cut(s) 537, 579, 607, 819, 966, 1008, 1036, 1251, 1269
DpnII GATC 9 cut(s) 535, 577, 605, 817, 964, 1006, 1034, 1249, 1267
Eam1104I CTCTTC 2 cut(s) 306, 748
EarI CTCTTC 2 cut(s) 306, 748
Ecl136II GAGCTC 1 cut(s) 783
Eco130I CCWWGG 2 cut(s) 65, 430
Eco24I GRGCYC 1 cut(s) 785
Eco31I GGTCTC 1 cut(s) 485
Eco32I GATATC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 783
Eco57I CTGAAG 5 cut(s) 9, 360, 615, 1044, 1197
EcoICRI GAGCTC 1 cut(s) 783
EcoRII CCWGG 4 cut(s) 553, 868, 982, 1300
EcoRV GATATC 1 cut(s) 238
EcoT14I CCWWGG 2 cut(s) 65, 430
EcoT38I GRGCYC 1 cut(s) 785
ErhI CCWWGG 2 cut(s) 65, 430
FaeI CATG 4 cut(s) 48, 69, 117, 152
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FatI CATG 4 cut(s) 44, 65, 113, 148
FauI CCCGC 1 cut(s) 592
FbaI TGATCA 2 cut(s) 577, 1267
FblI GTMKAC 1 cut(s) 176
FokI GGATG 4 cut(s) 236, 309, 852, 1284
FriOI GRGCYC 1 cut(s) 785
FspBI CTAG 4 cut(s) 275, 585, 1014, 1041
GsuI CTGGAG 2 cut(s) 870, 1302
HaeIII GGCC 1 cut(s) 118
HapII CCGG 2 cut(s) 488, 765
Hin1I GRCGYC 2 cut(s) 136, 1193
Hin1II CATG 4 cut(s) 48, 69, 117, 152
HinfI GANTC 8 cut(s) 145, 173, 228, 613, 737, 794, 1139, 1169
HpaII CCGG 2 cut(s) 488, 765
HphI GGTGA 3 cut(s) 358, 475, 1277
Hpy166II GTNNAC 5 cut(s) 177, 638, 875, 1067, 1307
Hpy188I TCNGA 4 cut(s) 51, 595, 1024, 1177
Hpy8I GTNNAC 5 cut(s) 177, 638, 875, 1067, 1307
Hpy99I CGWCG 1 cut(s) 1344
HpyAV CCTTC 6 cut(s) 335, 740, 852, 888, 1284, 1320
HpyCH4III ACNGT 5 cut(s) 402, 657, 721, 1086, 1153
HpyCH4IV ACGT 4 cut(s) 136, 307, 573, 1193
HpyCH4V TGCA 9 cut(s) 58, 152, 569, 623, 638, 704, 875, 1067, 1307
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 385, 629, 644, 1073
HpyF3I CTNAG 2 cut(s) 882, 1314
HpySE526I ACGT 4 cut(s) 136, 307, 573, 1193
Hsp92I GRCGYC 2 cut(s) 136, 1193
Hsp92II CATG 4 cut(s) 48, 69, 117, 152
Ksp22I TGATCA 2 cut(s) 577, 1267
KspI CCGCGG 1 cut(s) 1137
Kzo9I GATC 9 cut(s) 535, 577, 605, 817, 964, 1006, 1034, 1249, 1267
LguI GCTCTTC 1 cut(s) 748
LmnI GCTCC 4 cut(s) 127, 788, 851, 1283
Lsp1109I GCAGC 9 cut(s) 164, 400, 454, 634, 635, 656, 716, 840, 1085
LweI GCATC 4 cut(s) 108, 408, 691, 1336
MaeI CTAG 4 cut(s) 275, 585, 1014, 1041
MaeII ACGT 4 cut(s) 136, 307, 573, 1193
MaeIII GTNAC 3 cut(s) 229, 715, 1189
MalI GATC 9 cut(s) 537, 579, 607, 819, 966, 1008, 1036, 1251, 1269
MboI GATC 9 cut(s) 535, 577, 605, 817, 964, 1006, 1034, 1249, 1267
MflI RGATCY 4 cut(s) 535, 605, 964, 1034
MhlI GDGCHC 5 cut(s) 640, 785, 877, 1069, 1309
MluCI AATT 8 cut(s) 31, 210, 242, 405, 512, 769, 941, 1201
MlyI GAGTC 5 cut(s) 182, 237, 622, 803, 1178
MmeI TCCRAC 2 cut(s) 274, 810
MroXI GAANNNNTTC 1 cut(s) 607
MseI TTAA 3 cut(s) 404, 510, 933
MslI CAYNNNNRTG 2 cut(s) 870, 1302
MspA1I CMGCKG 4 cut(s) 155, 647, 1076, 1136
MspI CCGG 2 cut(s) 488, 765
MspR9I CCNGG 5 cut(s) 488, 555, 870, 984, 1302
MvaI CCWGG 4 cut(s) 555, 870, 984, 1302
MvnI CGCG 1 cut(s) 1136
MwoI GCNNNNNNNGC 5 cut(s) 105, 385, 629, 644, 1073
NciI CCSGG 1 cut(s) 488
NcoI CCATGG 1 cut(s) 65
NdeII GATC 9 cut(s) 535, 577, 605, 817, 964, 1006, 1034, 1249, 1267
NlaIII CATG 4 cut(s) 48, 69, 117, 152
NlaIV GGNNCC 2 cut(s) 165, 336
NmuCI GTSAC 3 cut(s) 229, 715, 1189
PciSI GCTCTTC 1 cut(s) 748
PcsI WCGNNNNNNNCGW 1 cut(s) 1336
PdmI GAANNNNTTC 1 cut(s) 607
PfeI GAWTC 3 cut(s) 145, 737, 1139
PinAI ACCGGT 1 cut(s) 764
PleI GAGTC 5 cut(s) 181, 236, 621, 802, 1177
PpsI GAGTC 5 cut(s) 181, 236, 621, 802, 1177
Psp124BI GAGCTC 1 cut(s) 785
Psp1406I AACGTT 1 cut(s) 573
Psp6I CCWGG 4 cut(s) 553, 868, 982, 1300
PspGI CCWGG 4 cut(s) 553, 868, 982, 1300
PspN4I GGNNCC 2 cut(s) 165, 336
PstNI CAGNNNCTG 1 cut(s) 400
PsuI RGATCY 4 cut(s) 535, 605, 964, 1034
PvuII CAGCTG 3 cut(s) 155, 647, 1076
RseI CAYNNNNRTG 2 cut(s) 870, 1302
SacI GAGCTC 1 cut(s) 785
SacII CCGCGG 1 cut(s) 1137
SapI GCTCTTC 1 cut(s) 748
SaqAI TTAA 3 cut(s) 404, 510, 933
Sau3AI GATC 9 cut(s) 535, 577, 605, 817, 964, 1006, 1034, 1249, 1267
SchI GAGTC 5 cut(s) 182, 237, 622, 803, 1178
ScrFI CCNGG 5 cut(s) 488, 555, 870, 984, 1302
SduI GDGCHC 5 cut(s) 640, 785, 877, 1069, 1309
SfaNI GCATC 4 cut(s) 108, 408, 691, 1336
SfcI CTRYAG 1 cut(s) 627
Sfr303I CCGCGG 1 cut(s) 1137
SgrBI CCGCGG 1 cut(s) 1137
SmiMI CAYNNNNRTG 2 cut(s) 870, 1302
Sse9I AATT 8 cut(s) 31, 210, 242, 405, 512, 769, 941, 1201
SsiI CCGC 4 cut(s) 599, 896, 1134, 1136
SspMI CTAG 4 cut(s) 275, 585, 1014, 1041
SstI GAGCTC 1 cut(s) 785
StyD4I CCNGG 5 cut(s) 486, 553, 868, 982, 1300
StyI CCWWGG 2 cut(s) 65, 430
TaaI ACNGT 5 cut(s) 402, 657, 721, 1086, 1153
TaiI ACGT 4 cut(s) 139, 310, 576, 1196
TaqI TCGA 3 cut(s) 102, 611, 1005
TasI AATT 8 cut(s) 31, 210, 242, 405, 512, 769, 941, 1201
TauI GCSGC 2 cut(s) 899, 1136
TfiI GAWTC 3 cut(s) 145, 737, 1139
Tru1I TTAA 3 cut(s) 404, 510, 933
Tru9I TTAA 3 cut(s) 404, 510, 933
TscAI CASTG 2 cut(s) 238, 781
TseFI GTSAC 3 cut(s) 229, 715, 1189
TseI GCWGC 9 cut(s) 152, 388, 467, 623, 644, 647, 704, 828, 1073
Tsp45I GTSAC 3 cut(s) 229, 715, 1189
TspDTI ATGAA 4 cut(s) 137, 477, 906, 1341
TspGWI ACGGA 2 cut(s) 399, 1345
TspRI CASTG 2 cut(s) 238, 781
VneI GTGCAC 4 cut(s) 636, 873, 1065, 1305
XapI RAATTY 4 cut(s) 210, 242, 512, 941
XbaI TCTAGA 2 cut(s) 584, 1013
XmiI GTMKAC 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 607
XspI CTAG 4 cut(s) 275, 585, 1014, 1041
ZraI GACGTC 2 cut(s) 137, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.