Rroxscaffold_6G00427740

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002815
Physical Location & Seq
Forward (+)
48304629 .. 48306824
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00427740.1

Sequence Viewer

Length: 1023 bp
ATGAGAAGAGGAGGAGGTTCTTCTGGGAATTACCGAAACCCATGTCTGACTATGCACCAGCCATGGGCTTCTCTGCTCGTATATGGTATCAAACGCATCGAGGGCAGGTCATGGCCTGCTCCCATTAGAGGACGTCTTTGGATTCATGCTGCTGGTAAGGTTCCAGAGGAGGCTACAGTCAAAGCAATGGAGGATTTCTATCGGGAAATTTATGCTGTGGATGGAGTCACTGATATCAAATTTCCCCAACACTATCCAGTTTCAAGACTGCTAGGGTGTGTTGAAGTAGTTGGATGTGTTAGACGCGAAGAGTTAGCTGGTTGGGAGATGGTTCCTGAAGGGGTGAGGCTGGAAGCACAAACCGATATGTGTTGGCTTTGCGAGCAGCCACAGAAACTGTTAATTCCGTTTGAGATGCGTGGCTACCAAGGTGTCTATAACTTGGAAAAGAAGATATATGAAGCTGCTATTAGAGGTCTCACCCCGGTTGAAGTTCCTCTCCCAGTTAAATTTCCACTTCCAAATCCACAAGATCCATTTTCCTTGAAGCCGGGGTCTATATCTGCAAACGTTAATCAATCTAGAACATCTGAAGCGGGAAGATCTTCGAGTCTCAATGCAGCTATAGCTGGTGCACGAGCAGCTGCTACACAAGCAATGACCGGTAATTTCATTGAGAGCTCCAATAAAGAGTCTATGACATCAAACAACAAAGATCAAATCAGCAGCCGTAATCAACCTTCTTCTGGAGTTATTAGACCTCACCCTGGTGCACCTTCTAAGATATATGAAGCTGCTATTAGAGGTCTTATCCCTGTTGAAGTTCCCCTTAAAGTCAAATTTCCAGTTCCAAATCCGGAAGATCCATTTTCCTTGAAGCCAGGATTTATATCTGCTAATGTCGATCAATCTAGAACATCTGAAGTGGTAAGATCTTCTAGTCTCAATACAGCCATAGCTGGTGCACAAGCAGCTGATACACAGTTCTCCAAAAAGCTCAAACTTTCCCAACTACCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

340

Amino Acids

37.21

Weight (kDa)

9.22

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 16 - 105 2.3e-08 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 96
AccII CGCG 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 856
AciI CCGC 1 cut(s) 596
AclI AACGTT 1 cut(s) 570
AclWI GGATC 2 cut(s) 527, 857
AcsI RAATTY 4 cut(s) 207, 239, 509, 839
AcuI CTGAAG 3 cut(s) 357, 612, 942
AcyI GRCGYC 1 cut(s) 133
AfiI CCNNNNNNNGG 4 cut(s) 64, 128, 746, 767
AgeI ACCGGT 1 cut(s) 662
AgsI TTSAA 6 cut(s) 264, 284, 491, 547, 821, 877
AjnI CCWGG 2 cut(s) 766, 880
AleI CACNNNNGTG 1 cut(s) 768
Alw21I GWGCWC 4 cut(s) 637, 683, 775, 967
Alw26I GTCTC 3 cut(s) 482, 617, 947
Alw44I GTGCAC 3 cut(s) 633, 771, 963
AlwI GGATC 2 cut(s) 527, 857
AlwNI CAGNNNCTG 1 cut(s) 397
Aor13HI TCCGGA 1 cut(s) 856
AoxI GGCC 1 cut(s) 113
ApaLI GTGCAC 3 cut(s) 633, 771, 963
ApeKI GCWGC 9 cut(s) 149, 385, 464, 620, 641, 644, 726, 794, 971
ApoI RAATTY 4 cut(s) 207, 239, 509, 839
ArsI GACNNNNNNTTYG 2 cut(s) 28, 60
AsiGI ACCGGT 1 cut(s) 662
Asp700I GAANNNNTTC 1 cut(s) 604
AsuC2I CCSGG 2 cut(s) 485, 552
AsuHPI GGTGA 3 cut(s) 355, 472, 755
BaeGI GKGCMC 3 cut(s) 637, 775, 967
BanII GRGCYC 1 cut(s) 683
BarI GAAGNNNNNNTAC 2 cut(s) 724, 756
BauI CACGAG 1 cut(s) 636
Bbv12I GWGCWC 4 cut(s) 637, 683, 775, 967
BbvI GCAGC 9 cut(s) 136, 397, 451, 631, 632, 653, 738, 781, 983
BccI CCATC 2 cut(s) 215, 322
BceAI ACGGC 1 cut(s) 714
BciT130I CCWGG 2 cut(s) 768, 882
BcnI CCSGG 2 cut(s) 485, 552
BcoDI GTCTC 3 cut(s) 482, 617, 947
BfaI CTAG 4 cut(s) 272, 582, 912, 939
BfmI CTRYAG 2 cut(s) 174, 624
BfuAI ACCTGC 1 cut(s) 96
BglII AGATCT 2 cut(s) 602, 932
BisI GCNGC 9 cut(s) 150, 386, 465, 621, 642, 645, 727, 795, 972
BlsI GCNGC 9 cut(s) 151, 387, 466, 622, 643, 646, 728, 796, 973
Bme1390I CCNGG 4 cut(s) 485, 552, 768, 882
BmiI GGNNCC 2 cut(s) 162, 333
BmrFI CCNGG 4 cut(s) 485, 552, 768, 882
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 2 cut(s) 105, 405
BmuI ACTGGG 1 cut(s) 497
BpmI CTGGAG 1 cut(s) 768
BpuMI CCSGG 2 cut(s) 485, 552
BsaBI GATNNNNATC 2 cut(s) 198, 889
BsaHI GRCGYC 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 5 cut(s) 62, 427, 483, 551, 766
BsaWI WCCGGW 2 cut(s) 662, 856
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 128, 746, 767
Bse118I RCCGGY 1 cut(s) 662
Bse1I ACTGG 3 cut(s) 257, 503, 845
Bse3DI GCAATG 2 cut(s) 192, 663
Bse8I GATNNNNATC 2 cut(s) 198, 889
BseAI TCCGGA 1 cut(s) 856
BseBI CCWGG 2 cut(s) 768, 882
BseDI CCNNGG 5 cut(s) 62, 427, 483, 551, 766
BseGI GGATG 2 cut(s) 226, 299
BseJI GATNNNNATC 2 cut(s) 198, 889
BseLI CCNNNNNNNGG 4 cut(s) 64, 128, 746, 767
BseMI GCAATG 2 cut(s) 192, 663
BseNI ACTGG 3 cut(s) 257, 503, 845
BseRI GAGGAG 3 cut(s) 24, 27, 182
BseSI GKGCMC 3 cut(s) 637, 775, 967
BseXI GCAGC 9 cut(s) 136, 397, 451, 631, 632, 653, 738, 781, 983
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 1 cut(s) 115
BshTI ACCGGT 1 cut(s) 662
BsiHKAI GWGCWC 4 cut(s) 637, 683, 775, 967
BsiSI CCGG 4 cut(s) 485, 551, 663, 857
BslI CCNNNNNNNGG 4 cut(s) 64, 128, 746, 767
BsmAI GTCTC 3 cut(s) 482, 617, 947
BsnI GGCC 1 cut(s) 115
Bso31I GGTCTC 1 cut(s) 482
Bsp1286I GDGCHC 4 cut(s) 637, 683, 775, 967
Bsp13I TCCGGA 1 cut(s) 856
Bsp143I GATC 6 cut(s) 532, 602, 715, 862, 904, 932
Bsp19I CCATGG 1 cut(s) 62
BspACI CCGC 1 cut(s) 596
BspANI GGCC 1 cut(s) 115
BspEI TCCGGA 1 cut(s) 856
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 2 cut(s) 162, 333
BspMI ACCTGC 1 cut(s) 96
BspPI GGATC 2 cut(s) 527, 857
BspTNI GGTCTC 1 cut(s) 482
BsrDI GCAATG 2 cut(s) 192, 663
BsrFI RCCGGY 1 cut(s) 662
BsrI ACTGG 3 cut(s) 257, 503, 845
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 5 cut(s) 62, 427, 483, 551, 766
BssMI GATC 6 cut(s) 532, 602, 715, 862, 904, 932
BssNI GRCGYC 1 cut(s) 133
BssSI CACGAG 1 cut(s) 636
BssT1I CCWWGG 2 cut(s) 62, 427
Bst2BI CACGAG 1 cut(s) 636
Bst2UI CCWGG 2 cut(s) 768, 882
Bst4CI ACNGT 3 cut(s) 178, 399, 984
Bst6I CTCTTC 1 cut(s) 303
BstACI GRCGYC 1 cut(s) 133
BstC8I GCNNGC 2 cut(s) 117, 383
BstDEI CTNAG 1 cut(s) 780
BstDSI CCRYGG 1 cut(s) 62
BstF5I GGATG 2 cut(s) 226, 299
BstFNI CGCG 1 cut(s) 306
BstKTI GATC 6 cut(s) 535, 605, 718, 865, 907, 935
BstMAI GTCTC 3 cut(s) 482, 617, 947
BstMBI GATC 6 cut(s) 532, 602, 715, 862, 904, 932
BstMWI GCNNNNNNNGC 6 cut(s) 102, 382, 626, 641, 653, 971
BstNI CCWGG 2 cut(s) 768, 882
BstSCI CCNGG 4 cut(s) 483, 550, 766, 880
BstSFI CTRYAG 2 cut(s) 174, 624
BstSLI GKGCMC 3 cut(s) 637, 775, 967
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 9 cut(s) 136, 397, 451, 631, 632, 653, 738, 781, 983
BstX2I RGATCY 4 cut(s) 532, 602, 862, 932
BstYI RGATCY 4 cut(s) 532, 602, 862, 932
BsuRI GGCC 1 cut(s) 115
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 2 cut(s) 226, 299
BtsIMutI CAGTG 1 cut(s) 228
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 2 cut(s) 117, 383
CaiI CAGNNNCTG 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 662
CseI GACGC 1 cut(s) 312
CspAI ACCGGT 1 cut(s) 662
CviAII CATG 4 cut(s) 42, 63, 111, 146
DdeI CTNAG 1 cut(s) 780
DpnI GATC 6 cut(s) 534, 604, 717, 864, 906, 934
DpnII GATC 6 cut(s) 532, 602, 715, 862, 904, 932
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 681
Eco130I CCWWGG 2 cut(s) 62, 427
Eco24I GRGCYC 1 cut(s) 683
Eco31I GGTCTC 1 cut(s) 482
Eco32I GATATC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 681
Eco57I CTGAAG 3 cut(s) 357, 612, 942
EcoICRI GAGCTC 1 cut(s) 681
EcoRII CCWGG 2 cut(s) 766, 880
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 62, 427
EcoT38I GRGCYC 1 cut(s) 683
ErhI CCWWGG 2 cut(s) 62, 427
FaeI CATG 4 cut(s) 45, 66, 114, 149
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FatI CATG 4 cut(s) 41, 62, 110, 145
FauI CCCGC 1 cut(s) 589
Fnu4HI GCNGC 9 cut(s) 150, 386, 465, 621, 642, 645, 727, 795, 972
FokI GGATG 2 cut(s) 233, 306
FriOI GRGCYC 1 cut(s) 683
Fsp4HI GCNGC 9 cut(s) 150, 386, 465, 621, 642, 645, 727, 795, 972
FspBI CTAG 4 cut(s) 272, 582, 912, 939
GluI GCNGC 9 cut(s) 150, 386, 465, 621, 642, 645, 727, 795, 972
GsuI CTGGAG 1 cut(s) 768
HaeIII GGCC 1 cut(s) 115
HapII CCGG 4 cut(s) 485, 551, 663, 857
HgaI GACGC 1 cut(s) 312
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 4 cut(s) 45, 66, 114, 149
HinfI GANTC 4 cut(s) 142, 225, 610, 692
HpaII CCGG 4 cut(s) 485, 551, 663, 857
HphI GGTGA 3 cut(s) 355, 472, 755
Hpy166II GTNNAC 3 cut(s) 635, 773, 965
Hpy188I TCNGA 3 cut(s) 48, 592, 922
Hpy188III TCNNGA 8 cut(s) 164, 203, 264, 335, 582, 747, 857, 912
Hpy8I GTNNAC 3 cut(s) 635, 773, 965
HpyAV CCTTC 3 cut(s) 332, 750, 786
HpyCH4III ACNGT 3 cut(s) 178, 399, 984
HpyCH4IV ACGT 2 cut(s) 133, 570
HpyCH4V TGCA 6 cut(s) 55, 566, 620, 635, 773, 965
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 382, 626, 641, 653, 971
HpyF3I CTNAG 1 cut(s) 780
HpySE526I ACGT 2 cut(s) 133, 570
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 4 cut(s) 45, 66, 114, 149
Kpn2I TCCGGA 1 cut(s) 856
Kzo9I GATC 6 cut(s) 532, 602, 715, 862, 904, 932
LmnI GCTCC 2 cut(s) 124, 686
Lsp1109I GCAGC 9 cut(s) 136, 397, 451, 631, 632, 653, 738, 781, 983
LweI GCATC 2 cut(s) 105, 405
MaeI CTAG 4 cut(s) 272, 582, 912, 939
MaeII ACGT 2 cut(s) 133, 570
MaeIII GTNAC 1 cut(s) 226
MalI GATC 6 cut(s) 534, 604, 717, 864, 906, 934
MboI GATC 6 cut(s) 532, 602, 715, 862, 904, 932
MboII GAAGA 9 cut(s) 12, 18, 320, 463, 597, 612, 735, 872, 927
MflI RGATCY 4 cut(s) 532, 602, 862, 932
MhlI GDGCHC 4 cut(s) 637, 683, 775, 967
MluCI AATT 7 cut(s) 28, 207, 239, 402, 509, 667, 839
MlyI GAGTC 3 cut(s) 234, 619, 701
MmeI TCCRAC 1 cut(s) 271
MroI TCCGGA 1 cut(s) 856
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 4 cut(s) 401, 507, 573, 831
MslI CAYNNNNRTG 1 cut(s) 768
MspA1I CMGCKG 2 cut(s) 644, 974
MspI CCGG 4 cut(s) 485, 551, 663, 857
MspR9I CCNGG 4 cut(s) 485, 552, 768, 882
MvaI CCWGG 2 cut(s) 768, 882
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 6 cut(s) 102, 382, 626, 641, 653, 971
NciI CCSGG 2 cut(s) 485, 552
NcoI CCATGG 1 cut(s) 62
NdeII GATC 6 cut(s) 532, 602, 715, 862, 904, 932
NlaIII CATG 4 cut(s) 45, 66, 114, 149
NlaIV GGNNCC 2 cut(s) 162, 333
NmuCI GTSAC 1 cut(s) 226
OliI CACNNNNGTG 1 cut(s) 768
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 1 cut(s) 142
PinAI ACCGGT 1 cut(s) 662
PkrI GCNGC 9 cut(s) 151, 387, 466, 622, 643, 646, 728, 796, 973
PleI GAGTC 3 cut(s) 233, 618, 700
PpsI GAGTC 3 cut(s) 233, 618, 700
Psp124BI GAGCTC 1 cut(s) 683
Psp1406I AACGTT 1 cut(s) 570
Psp6I CCWGG 2 cut(s) 766, 880
PspGI CCWGG 2 cut(s) 766, 880
PspN4I GGNNCC 2 cut(s) 162, 333
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 4 cut(s) 532, 602, 862, 932
PvuII CAGCTG 2 cut(s) 644, 974
RseI CAYNNNNRTG 1 cut(s) 768
SacI GAGCTC 1 cut(s) 683
SaqAI TTAA 4 cut(s) 401, 507, 573, 831
SatI GCNGC 9 cut(s) 150, 386, 465, 621, 642, 645, 727, 795, 972
Sau3AI GATC 6 cut(s) 532, 602, 715, 862, 904, 932
SchI GAGTC 3 cut(s) 234, 619, 701
ScrFI CCNGG 4 cut(s) 485, 552, 768, 882
SduI GDGCHC 4 cut(s) 637, 683, 775, 967
SfaNI GCATC 2 cut(s) 105, 405
SfcI CTRYAG 2 cut(s) 174, 624
SmiMI CAYNNNNRTG 1 cut(s) 768
Sse9I AATT 7 cut(s) 28, 207, 239, 402, 509, 667, 839
SsiI CCGC 1 cut(s) 596
SspMI CTAG 4 cut(s) 272, 582, 912, 939
SstI GAGCTC 1 cut(s) 683
StyD4I CCNGG 4 cut(s) 483, 550, 766, 880
StyI CCWWGG 2 cut(s) 62, 427
TaaI ACNGT 3 cut(s) 178, 399, 984
TaiI ACGT 2 cut(s) 136, 573
TaqI TCGA 3 cut(s) 99, 608, 903
TasI AATT 7 cut(s) 28, 207, 239, 402, 509, 667, 839
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 4 cut(s) 401, 507, 573, 831
Tru9I TTAA 4 cut(s) 401, 507, 573, 831
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 9 cut(s) 149, 385, 464, 620, 641, 644, 726, 794, 971
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 4 cut(s) 134, 474, 661, 804
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 1 cut(s) 235
VneI GTGCAC 3 cut(s) 633, 771, 963
XapI RAATTY 4 cut(s) 207, 239, 509, 839
XbaI TCTAGA 2 cut(s) 581, 911
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 4 cut(s) 272, 582, 912, 939
ZraI GACGTC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.