Rh3AG023300

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002815
Physical Location & Seq
Forward (+)
1571236 .. 1574447
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG023300.1

Sequence Viewer

Length: 1170 bp
ATGGAGGATTTCTATCGGGAAATTTATGCCGTGGATGGAGTCACTGATATCAAATTTCCCCAACACTATCCAGTTTCAAGACTGCTAGGGTGTGTTGAAGTAGTTGGATGTGTTAGACGCGAAGAGTTAGCTGGTTGGGAGATGGTTCCTGAAGGGGTGAGGCTGGAAGCACAAACCGATATGTGTTGGCTTTGCGAGCAGCCACAGAAACTGTTAATTCCGTTTGAGATGCGTGGCTACCAAGGTGTCTATAACTTGGAAAAGAAGATATATGAAGCTGCTATTAGAGGTCTCACCCCGGTTGAAGTTCCTCTCCCAGTTAAATTTCCACTTCCAAATCCACAAGATCCATTTTCCTTGAAGCCAGGGTCTATATCTGCAAACGTTGATCAATCTAGAACATCTGAAGCGGGAAGATCTTCGAGTCTCAATGCAGCTATAGCTGGTGCACGAGCAGCTGCTACACAGTTCTCCAAAAAACCTCAAACTTTCCCAACTACCTCCCTAAATGATGCAGCCAAATCTGTGACAGTTCCAATCAAGAGTGAATCTTTTGAAGGAGGAAGAGCAATGACCGGTAATTTCAGTGAGAGCTCCAACAAAGAGTCAATGACATCAAATAACAAAGATCAAATCAGCAGCCGTAATCAACCTTCTTCTGGAGCTATTAGACCTCATCCTGGTGCACCTTCTAAGATATATGAAGCAGCTATTAGAGGTCTTACCCCTGTTGAAGTTCCCCTTAAAGTCAAATTTCCAGTTCCAAATCCAGAAGATCCATTTTCCTTGAAGCCAGGATTTATATCTGCTAATGTCGATCAATCTAGAACATCTGAAGTGGTAAGATCTTCTAGTCTCAATACAGCCATAGCTGGTGCACAAGCAGCTGATACACAGTTCTCCAAAAAAACTCAAACTTTCCCAACTACCTCCCTAAATAATGCCGCGGAATCTGTGATGACAGTTCCAATCAGGAGTGAGTCTTCTGAAGTGGGTAAAGTGACGTCTGCTAATTTTATTGAGAGTTCTAATAAGAGGTCTATGACATCAAATAACAAAGATCAAATCAGCAGAGGTGATCAACCTTCTTCTGGAGCTTTTAGACCTCATCCTGGTGCACCTTCTAAGTATGTTTATGAACGGAGGAGGCGACGAGATGCCCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

389

Amino Acids

42.47

Weight (kDa)

9.02

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1007
AccII CGCG 2 cut(s) 120, 947
AciI CCGC 3 cut(s) 410, 945, 947
AclI AACGTT 1 cut(s) 384
AclWI GGATC 2 cut(s) 341, 770
AcsI RAATTY 4 cut(s) 21, 53, 323, 752
AcuI CTGAAG 4 cut(s) 171, 426, 855, 1008
AcyI GRCGYC 1 cut(s) 1004
AfiI CCNNNNNNNGG 4 cut(s) 659, 680, 1091, 1112
AgeI ACCGGT 1 cut(s) 575
AgsI TTSAA 7 cut(s) 78, 98, 305, 361, 557, 734, 790
AjnI CCWGG 4 cut(s) 364, 679, 793, 1111
Alw21I GWGCWC 5 cut(s) 451, 596, 688, 880, 1120
Alw26I GTCTC 3 cut(s) 296, 431, 860
Alw44I GTGCAC 4 cut(s) 447, 684, 876, 1116
AlwI GGATC 2 cut(s) 341, 770
AlwNI CAGNNNCTG 1 cut(s) 211
ApaLI GTGCAC 4 cut(s) 447, 684, 876, 1116
ApeKI GCWGC 9 cut(s) 199, 278, 434, 455, 458, 515, 639, 707, 884
ApoI RAATTY 4 cut(s) 21, 53, 323, 752
AsiGI ACCGGT 1 cut(s) 575
Asp700I GAANNNNTTC 1 cut(s) 418
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 3 cut(s) 169, 286, 1088
BaeGI GKGCMC 4 cut(s) 451, 688, 880, 1120
BanII GRGCYC 1 cut(s) 596
BarI GAAGNNNNNNTAC 2 cut(s) 637, 669
BauI CACGAG 1 cut(s) 450
BbsI GAAGAC 1 cut(s) 975
Bbv12I GWGCWC 5 cut(s) 451, 596, 688, 880, 1120
BbvI GCAGC 9 cut(s) 211, 265, 445, 446, 467, 527, 651, 719, 896
BccI CCATC 2 cut(s) 29, 136
BceAI ACGGC 2 cut(s) 14, 627
BciT130I CCWGG 4 cut(s) 366, 681, 795, 1113
BclI TGATCA 2 cut(s) 388, 1078
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 3 cut(s) 296, 431, 860
BfaI CTAG 4 cut(s) 86, 396, 825, 852
BfmI CTRYAG 1 cut(s) 438
BglII AGATCT 2 cut(s) 416, 845
Bme1390I CCNGG 5 cut(s) 299, 366, 681, 795, 1113
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 5 cut(s) 299, 366, 681, 795, 1113
BmrI ACTGGG 2 cut(s) 311, 1156
BmsI GCATC 3 cut(s) 219, 502, 1147
BmuI ACTGGG 2 cut(s) 311, 1156
BpiI GAAGAC 1 cut(s) 975
BpmI CTGGAG 2 cut(s) 681, 1113
BpuMI CCSGG 1 cut(s) 299
BsaBI GATNNNNATC 2 cut(s) 12, 802
BsaHI GRCGYC 1 cut(s) 1004
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 5 cut(s) 30, 241, 297, 365, 945
BsaWI WCCGGW 1 cut(s) 575
Bsc4I CCNNNNNNNGG 4 cut(s) 659, 680, 1091, 1112
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 4 cut(s) 71, 317, 758, 1162
Bse3DI GCAATG 1 cut(s) 576
Bse8I GATNNNNATC 2 cut(s) 12, 802
BseBI CCWGG 4 cut(s) 366, 681, 795, 1113
BseDI CCNNGG 5 cut(s) 30, 241, 297, 365, 945
BseGI GGATG 4 cut(s) 40, 113, 676, 1108
BseJI GATNNNNATC 2 cut(s) 12, 802
BseLI CCNNNNNNNGG 4 cut(s) 659, 680, 1091, 1112
BseMI GCAATG 1 cut(s) 576
BseNI ACTGG 4 cut(s) 71, 317, 758, 1162
BseRI GAGGAG 1 cut(s) 1159
BseSI GKGCMC 4 cut(s) 451, 688, 880, 1120
BseXI GCAGC 9 cut(s) 211, 265, 445, 446, 467, 527, 651, 719, 896
Bsh1236I CGCG 2 cut(s) 120, 947
BshTI ACCGGT 1 cut(s) 575
BsiHKAI GWGCWC 5 cut(s) 451, 596, 688, 880, 1120
BsiSI CCGG 2 cut(s) 299, 576
BslI CCNNNNNNNGG 4 cut(s) 659, 680, 1091, 1112
BsmAI GTCTC 3 cut(s) 296, 431, 860
Bso31I GGTCTC 1 cut(s) 296
Bsp1286I GDGCHC 5 cut(s) 451, 596, 688, 880, 1120
Bsp143I GATC 9 cut(s) 346, 388, 416, 628, 775, 817, 845, 1060, 1078
BspACI CCGC 3 cut(s) 410, 945, 947
BspFNI CGCG 2 cut(s) 120, 947
BspLI GGNNCC 1 cut(s) 147
BspPI GGATC 2 cut(s) 341, 770
BspQI GCTCTTC 1 cut(s) 559
BspTNI GGTCTC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 576
BsrFI RCCGGY 1 cut(s) 575
BsrI ACTGG 4 cut(s) 71, 317, 758, 1162
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 5 cut(s) 30, 241, 297, 365, 945
BssMI GATC 9 cut(s) 346, 388, 416, 628, 775, 817, 845, 1060, 1078
BssNI GRCGYC 1 cut(s) 1004
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 241
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 4 cut(s) 366, 681, 795, 1113
Bst4CI ACNGT 5 cut(s) 213, 468, 532, 897, 964
Bst6I CTCTTC 2 cut(s) 117, 559
BstACI GRCGYC 1 cut(s) 1004
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 2 cut(s) 693, 1125
BstDSI CCRYGG 2 cut(s) 30, 945
BstF5I GGATG 4 cut(s) 40, 113, 676, 1108
BstFNI CGCG 2 cut(s) 120, 947
BstKTI GATC 9 cut(s) 349, 391, 419, 631, 778, 820, 848, 1063, 1081
BstMAI GTCTC 3 cut(s) 296, 431, 860
BstMBI GATC 9 cut(s) 346, 388, 416, 628, 775, 817, 845, 1060, 1078
BstMWI GCNNNNNNNGC 4 cut(s) 196, 440, 455, 884
BstNI CCWGG 4 cut(s) 366, 681, 795, 1113
BstSCI CCNGG 5 cut(s) 297, 364, 679, 793, 1111
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 4 cut(s) 451, 688, 880, 1120
BstUI CGCG 2 cut(s) 120, 947
BstV1I GCAGC 9 cut(s) 211, 265, 445, 446, 467, 527, 651, 719, 896
BstV2I GAAGAC 1 cut(s) 975
BstX2I RGATCY 4 cut(s) 346, 416, 775, 845
BstYI RGATCY 4 cut(s) 346, 416, 775, 845
BtgI CCRYGG 2 cut(s) 30, 945
BtsCI GGATG 4 cut(s) 40, 113, 676, 1108
BtsIMutI CAGTG 2 cut(s) 42, 592
Cac8I GCNNGC 1 cut(s) 197
CaiI CAGNNNCTG 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 575
Cfr42I CCGCGG 1 cut(s) 948
CseI GACGC 1 cut(s) 126
CspAI ACCGGT 1 cut(s) 575
DdeI CTNAG 2 cut(s) 693, 1125
DpnI GATC 9 cut(s) 348, 390, 418, 630, 777, 819, 847, 1062, 1080
DpnII GATC 9 cut(s) 346, 388, 416, 628, 775, 817, 845, 1060, 1078
Eam1104I CTCTTC 2 cut(s) 117, 559
EarI CTCTTC 2 cut(s) 117, 559
Ecl136II GAGCTC 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 596
Eco31I GGTCTC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 594
Eco57I CTGAAG 4 cut(s) 171, 426, 855, 1008
EcoICRI GAGCTC 1 cut(s) 594
EcoRII CCWGG 4 cut(s) 364, 679, 793, 1111
EcoRV GATATC 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 596
ErhI CCWWGG 1 cut(s) 241
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FauI CCCGC 1 cut(s) 403
FbaI TGATCA 2 cut(s) 388, 1078
FokI GGATG 4 cut(s) 47, 120, 663, 1095
FriOI GRGCYC 1 cut(s) 596
FspBI CTAG 4 cut(s) 86, 396, 825, 852
GsuI CTGGAG 2 cut(s) 681, 1113
HapII CCGG 2 cut(s) 299, 576
HgaI GACGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 1004
HinfI GANTC 6 cut(s) 39, 424, 548, 605, 950, 980
HpaII CCGG 2 cut(s) 299, 576
HphI GGTGA 3 cut(s) 169, 286, 1088
Hpy166II GTNNAC 4 cut(s) 449, 686, 878, 1118
Hpy188I TCNGA 3 cut(s) 406, 835, 988
Hpy8I GTNNAC 4 cut(s) 449, 686, 878, 1118
Hpy99I CGWCG 1 cut(s) 1155
HpyAV CCTTC 6 cut(s) 146, 551, 663, 699, 1095, 1131
HpyCH4III ACNGT 5 cut(s) 213, 468, 532, 897, 964
HpyCH4IV ACGT 2 cut(s) 384, 1004
HpyCH4V TGCA 7 cut(s) 380, 434, 449, 515, 686, 878, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 440, 455, 884
HpyF3I CTNAG 2 cut(s) 693, 1125
HpySE526I ACGT 2 cut(s) 384, 1004
Hsp92I GRCGYC 1 cut(s) 1004
Ksp22I TGATCA 2 cut(s) 388, 1078
KspI CCGCGG 1 cut(s) 948
Kzo9I GATC 9 cut(s) 346, 388, 416, 628, 775, 817, 845, 1060, 1078
LguI GCTCTTC 1 cut(s) 559
LmnI GCTCC 3 cut(s) 599, 662, 1094
Lsp1109I GCAGC 9 cut(s) 211, 265, 445, 446, 467, 527, 651, 719, 896
LweI GCATC 3 cut(s) 219, 502, 1147
MaeI CTAG 4 cut(s) 86, 396, 825, 852
MaeII ACGT 2 cut(s) 384, 1004
MaeIII GTNAC 3 cut(s) 40, 526, 1000
MalI GATC 9 cut(s) 348, 390, 418, 630, 777, 819, 847, 1062, 1080
MboI GATC 9 cut(s) 346, 388, 416, 628, 775, 817, 845, 1060, 1078
MflI RGATCY 4 cut(s) 346, 416, 775, 845
MhlI GDGCHC 5 cut(s) 451, 596, 688, 880, 1120
MluCI AATT 7 cut(s) 21, 53, 216, 323, 580, 752, 1012
MlyI GAGTC 4 cut(s) 48, 433, 614, 989
MmeI TCCRAC 2 cut(s) 85, 621
MroXI GAANNNNTTC 1 cut(s) 418
MseI TTAA 3 cut(s) 215, 321, 744
MslI CAYNNNNRTG 2 cut(s) 681, 1113
MspA1I CMGCKG 3 cut(s) 458, 887, 947
MspI CCGG 2 cut(s) 299, 576
MspR9I CCNGG 5 cut(s) 299, 366, 681, 795, 1113
MvaI CCWGG 4 cut(s) 366, 681, 795, 1113
MvnI CGCG 2 cut(s) 120, 947
MwoI GCNNNNNNNGC 4 cut(s) 196, 440, 455, 884
NciI CCSGG 1 cut(s) 299
NdeII GATC 9 cut(s) 346, 388, 416, 628, 775, 817, 845, 1060, 1078
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 3 cut(s) 40, 526, 1000
PciSI GCTCTTC 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 1147
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 2 cut(s) 548, 950
PinAI ACCGGT 1 cut(s) 575
PleI GAGTC 4 cut(s) 47, 432, 613, 988
PpsI GAGTC 4 cut(s) 47, 432, 613, 988
Psp124BI GAGCTC 1 cut(s) 596
Psp1406I AACGTT 1 cut(s) 384
Psp6I CCWGG 4 cut(s) 364, 679, 793, 1111
PspGI CCWGG 4 cut(s) 364, 679, 793, 1111
PspN4I GGNNCC 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 4 cut(s) 346, 416, 775, 845
PvuII CAGCTG 2 cut(s) 458, 887
RseI CAYNNNNRTG 2 cut(s) 681, 1113
SacI GAGCTC 1 cut(s) 596
SacII CCGCGG 1 cut(s) 948
SapI GCTCTTC 1 cut(s) 559
SaqAI TTAA 3 cut(s) 215, 321, 744
Sau3AI GATC 9 cut(s) 346, 388, 416, 628, 775, 817, 845, 1060, 1078
SchI GAGTC 4 cut(s) 48, 433, 614, 989
ScrFI CCNGG 5 cut(s) 299, 366, 681, 795, 1113
SduI GDGCHC 5 cut(s) 451, 596, 688, 880, 1120
SfaNI GCATC 3 cut(s) 219, 502, 1147
SfcI CTRYAG 1 cut(s) 438
Sfr303I CCGCGG 1 cut(s) 948
SgrBI CCGCGG 1 cut(s) 948
SmiMI CAYNNNNRTG 2 cut(s) 681, 1113
Sse9I AATT 7 cut(s) 21, 53, 216, 323, 580, 752, 1012
SsiI CCGC 3 cut(s) 410, 945, 947
SspMI CTAG 4 cut(s) 86, 396, 825, 852
SstI GAGCTC 1 cut(s) 596
StyD4I CCNGG 5 cut(s) 297, 364, 679, 793, 1111
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 5 cut(s) 213, 468, 532, 897, 964
TaiI ACGT 2 cut(s) 387, 1007
TaqI TCGA 2 cut(s) 422, 816
TasI AATT 7 cut(s) 21, 53, 216, 323, 580, 752, 1012
TauI GCSGC 1 cut(s) 947
TfiI GAWTC 2 cut(s) 548, 950
Tru1I TTAA 3 cut(s) 215, 321, 744
Tru9I TTAA 3 cut(s) 215, 321, 744
TscAI CASTG 2 cut(s) 49, 592
TseFI GTSAC 3 cut(s) 40, 526, 1000
TseI GCWGC 9 cut(s) 199, 278, 434, 455, 458, 515, 639, 707, 884
Tsp45I GTSAC 3 cut(s) 40, 526, 1000
TspDTI ATGAA 3 cut(s) 288, 717, 1152
TspGWI ACGGA 2 cut(s) 210, 1156
TspRI CASTG 2 cut(s) 49, 592
VneI GTGCAC 4 cut(s) 447, 684, 876, 1116
XapI RAATTY 4 cut(s) 21, 53, 323, 752
XbaI TCTAGA 2 cut(s) 395, 824
XmnI GAANNNNTTC 1 cut(s) 418
XspI CTAG 4 cut(s) 86, 396, 825, 852
ZraI GACGTC 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.