MD12G1246100.v1.1

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002815
Physical Location & Seq
Reverse (-)
31631518 .. 31635170
3653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1246100.v1.1.491

Sequence Viewer

Length: 915 bp
ATGAGAAGAGGAGGAGGAGGAGGAGGAGGACATGCTTCTTCTGGGAATTACAGAAACCCATGTTTGACTATGCACCAACCATGGGCTTCTTTACTGGTTTATGGCATCAAGCGCGTCGAGGGCAGGTCCTGGCCTTCCCCTATCAGAGGCCGTCTTTGGATTCATGCCGCTGGTAAGGTTCCTGATGAGGCTACAATTAAAGCAATGGAAGAATTTTACAGGGAAATTTATGCAGTTGATGGAATCACGGATCTTAAATTTCCAGAACATTATCCGGTTTCAAGACTTTTAGGGTGTGTCGAAGTGGTTGGATGTCTTAGACGAGAAGAACTTGCATCCTGGGAGATGGTACCTGAAGGGGTGAGGCTGGAAGCATTAACTGATATGTGTTGGCTTTGTGAGCAACCACAGAAACTGTTAGTTCCATTTGAGATGCGTGGCTACCAAGGAGTCTATAACTTGGAAAAGAAGATATATGAAGGTGCAATTAGAGGTCTTTCCCCAGTTAATAGTCCCCTTCCAGTAAAGTTTCCACTTCCAAATCCACAAGATCCATTTTCATTGAAACCAGGATCTATCTCTGTGCATGTCCCTGTATCTAGAACATCTGAAGTTGAGATATCATCGAGTCTCACTGCAGCCATAGCTGGTGCCCGTGCTGCAGCTACACAGTTCTCCAAGAAAGTTCAAGATGCACAGACATCTGTAACGACCCTTCCATTAAAGGGTCAATCTGTTGAAGAGGATACAATGCCATCTGCTAACCTCAGTGAGACCTCTAATAAAGGGGCATTGACATCTAATAACAAGGAGCAAATAAGTCCCATTAAGCACAAGGGAGTCGGCAGCCACAGCCAACCTTATTCTGGAGGTTTGAGACCTCAACCTGGTGCACCTTCTAAGCGTTCAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

305

Amino Acids

33.05

Weight (kDa)

9.02

Isoelectric Point (pI)

60.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 22 - 125 6.1e-09 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 114
Acc65I GGTACC 1 cut(s) 349
AccB1I GGYRCC 2 cut(s) 349, 650
AccII CGCG 1 cut(s) 114
AciI CCGC 1 cut(s) 168
AclWI GGATC 3 cut(s) 258, 545, 580
AcsI RAATTY 3 cut(s) 212, 225, 257
AcuI CTGAAG 2 cut(s) 375, 630
AfaI GTAC 1 cut(s) 351
AfiI CCNNNNNNNGG 5 cut(s) 82, 146, 725, 866, 887
AgsI TTSAA 4 cut(s) 282, 565, 689, 740
AjnI CCWGG 4 cut(s) 128, 338, 568, 886
AluBI AGCT 2 cut(s) 647, 665
AluI AGCT 2 cut(s) 647, 665
Alw21I GWGCWC 1 cut(s) 895
Alw26I GTCTC 3 cut(s) 635, 767, 871
Alw44I GTGCAC 1 cut(s) 891
AlwI GGATC 3 cut(s) 258, 545, 580
AlwNI CAGNNNCTG 2 cut(s) 129, 415
AoxI GGCC 2 cut(s) 131, 148
ApaLI GTGCAC 1 cut(s) 891
ApeKI GCWGC 4 cut(s) 638, 659, 662, 846
ApoI RAATTY 3 cut(s) 212, 225, 257
Asp718I GGTACC 1 cut(s) 349
AspLEI GCGC 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 373
AvaII GGWCC 1 cut(s) 126
BaeGI GKGCMC 2 cut(s) 655, 895
BanI GGYRCC 2 cut(s) 349, 650
BarI GAAGNNNNNNTAC 2 cut(s) 699, 731
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 4 cut(s) 646, 650, 674, 858
BccI CCATC 3 cut(s) 233, 340, 763
BceAI ACGGC 1 cut(s) 135
BciT130I CCWGG 4 cut(s) 130, 340, 570, 888
BciVI GTATCC 1 cut(s) 739
BcoDI GTCTC 3 cut(s) 635, 767, 871
BfaI CTAG 2 cut(s) 600, 913
BfmI CTRYAG 2 cut(s) 636, 660
BfuAI ACCTGC 1 cut(s) 114
BfuI GTATCC 1 cut(s) 739
BisI GCNGC 5 cut(s) 168, 639, 660, 663, 847
BlsI GCNGC 5 cut(s) 169, 640, 661, 664, 848
Bme1390I CCNGG 4 cut(s) 130, 340, 570, 888
Bme18I GGWCC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 3 cut(s) 180, 351, 652
BmrFI CCNGG 4 cut(s) 130, 340, 570, 888
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 4 cut(s) 114, 344, 423, 682
BmuI ACTGGG 1 cut(s) 497
BpmI CTGGAG 1 cut(s) 888
BsaI GGTCTC 2 cut(s) 767, 871
BsaJI CCNNGG 3 cut(s) 80, 339, 445
BsaWI WCCGGW 1 cut(s) 274
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 146, 725, 866, 887
Bse1I ACTGG 3 cut(s) 99, 503, 521
Bse3DI GCAATG 1 cut(s) 210
BseBI CCWGG 4 cut(s) 130, 340, 570, 888
BseDI CCNNGG 3 cut(s) 80, 339, 445
BseGI GGATG 2 cut(s) 317, 335
BseLI CCNNNNNNNGG 5 cut(s) 82, 146, 725, 866, 887
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 781
BseNI ACTGG 3 cut(s) 99, 503, 521
BseRI GAGGAG 6 cut(s) 24, 27, 30, 33, 36, 39
BseSI GKGCMC 2 cut(s) 655, 895
BseXI GCAGC 4 cut(s) 646, 650, 674, 858
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 2 cut(s) 133, 150
BshNI GGYRCC 2 cut(s) 349, 650
BsiHKAI GWGCWC 1 cut(s) 895
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 3 cut(s) 498, 575, 807
BslI CCNNNNNNNGG 5 cut(s) 82, 146, 725, 866, 887
BsmAI GTCTC 3 cut(s) 635, 767, 871
BsmFI GGGAC 3 cut(s) 498, 575, 807
BsnI GGCC 2 cut(s) 133, 150
Bso31I GGTCTC 2 cut(s) 767, 871
Bsp1286I GDGCHC 2 cut(s) 655, 895
Bsp143I GATC 3 cut(s) 250, 550, 572
Bsp19I CCATGG 1 cut(s) 80
BspACI CCGC 1 cut(s) 168
BspANI GGCC 2 cut(s) 133, 150
BspCNI CTCAG 1 cut(s) 780
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 3 cut(s) 180, 351, 652
BspMAI CTGCAG 2 cut(s) 640, 664
BspMI ACCTGC 1 cut(s) 114
BspPI GGATC 3 cut(s) 258, 545, 580
BspT107I GGYRCC 2 cut(s) 349, 650
BspTNI GGTCTC 2 cut(s) 767, 871
BsrDI GCAATG 1 cut(s) 210
BsrI ACTGG 3 cut(s) 99, 503, 521
BssECI CCNNGG 3 cut(s) 80, 339, 445
BssMI GATC 3 cut(s) 250, 550, 572
BssT1I CCWWGG 2 cut(s) 80, 445
Bst2UI CCWGG 4 cut(s) 130, 340, 570, 888
Bst4CI ACNGT 2 cut(s) 417, 672
Bst6I CTCTTC 1 cut(s) 735
BstDEI CTNAG 3 cut(s) 317, 767, 900
BstDSI CCRYGG 1 cut(s) 80
BstENI CCTNNNNNAGG 1 cut(s) 144
BstF5I GGATG 2 cut(s) 317, 335
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 3 cut(s) 253, 553, 575
BstMAI GTCTC 3 cut(s) 635, 767, 871
BstMBI GATC 3 cut(s) 250, 550, 572
BstMWI GCNNNNNNNGC 6 cut(s) 111, 120, 400, 644, 659, 852
BstNI CCWGG 4 cut(s) 130, 340, 570, 888
BstNSI RCATGY 2 cut(s) 35, 590
BstSCI CCNGG 4 cut(s) 128, 338, 568, 886
BstSFI CTRYAG 2 cut(s) 636, 660
BstSLI GKGCMC 2 cut(s) 655, 895
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 4 cut(s) 646, 650, 674, 858
BstX2I RGATCY 3 cut(s) 250, 550, 572
BstYI RGATCY 3 cut(s) 250, 550, 572
BsuI GTATCC 1 cut(s) 739
BsuRI GGCC 2 cut(s) 133, 150
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 2 cut(s) 317, 335
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 2 cut(s) 633, 775
BveI ACCTGC 1 cut(s) 114
CaiI CAGNNNCTG 2 cut(s) 129, 415
CfoI GCGC 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 126
CseI GACGC 1 cut(s) 103
CsiI ACCWGGT 1 cut(s) 886
Csp6I GTAC 1 cut(s) 350
CviAII CATG 5 cut(s) 32, 60, 81, 164, 587
CviQI GTAC 1 cut(s) 350
DdeI CTNAG 3 cut(s) 317, 767, 900
DpnI GATC 3 cut(s) 252, 552, 574
DpnII GATC 3 cut(s) 250, 550, 572
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
Eco130I CCWWGG 2 cut(s) 80, 445
Eco31I GGTCTC 2 cut(s) 767, 871
Eco32I GATATC 1 cut(s) 621
Eco47I GGWCC 1 cut(s) 126
Eco57I CTGAAG 2 cut(s) 375, 630
EcoNI CCTNNNNNAGG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 126
EcoRII CCWGG 4 cut(s) 128, 338, 568, 886
EcoRV GATATC 1 cut(s) 621
EcoT14I CCWWGG 2 cut(s) 80, 445
ErhI CCWWGG 2 cut(s) 80, 445
FaeI CATG 5 cut(s) 35, 63, 84, 167, 590
FaqI GGGAC 3 cut(s) 498, 575, 807
FatI CATG 5 cut(s) 31, 59, 80, 163, 586
Fnu4HI GCNGC 5 cut(s) 168, 639, 660, 663, 847
FokI GGATG 2 cut(s) 322, 324
Fsp4HI GCNGC 5 cut(s) 168, 639, 660, 663, 847
FspBI CTAG 2 cut(s) 600, 913
GlaI GCGC 1 cut(s) 113
GluI GCNGC 5 cut(s) 168, 639, 660, 663, 847
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 2 cut(s) 133, 150
HapII CCGG 1 cut(s) 275
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 114
Hin1II CATG 5 cut(s) 35, 63, 84, 167, 590
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HinfI GANTC 5 cut(s) 160, 243, 450, 628, 840
HpaII CCGG 1 cut(s) 275
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 1 cut(s) 893
Hpy188I TCNGA 2 cut(s) 146, 610
Hpy188III TCNNGA 6 cut(s) 182, 263, 282, 600, 689, 867
Hpy8I GTNNAC 1 cut(s) 893
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 6 cut(s) 144, 350, 473, 527, 725, 906
HpyCH4III ACNGT 2 cut(s) 417, 672
HpyCH4V TGCA 9 cut(s) 73, 233, 335, 485, 586, 638, 662, 695, 893
HpyF10VI GCNNNNNNNGC 6 cut(s) 111, 120, 400, 644, 659, 852
HpyF3I CTNAG 3 cut(s) 317, 767, 900
Hsp92II CATG 5 cut(s) 35, 63, 84, 167, 590
HspAI GCGC 1 cut(s) 112
KpnI GGTACC 1 cut(s) 353
Kzo9I GATC 3 cut(s) 250, 550, 572
LmnI GCTCC 1 cut(s) 811
Lsp1109I GCAGC 4 cut(s) 646, 650, 674, 858
LweI GCATC 4 cut(s) 114, 344, 423, 682
MabI ACCWGGT 1 cut(s) 886
MaeI CTAG 2 cut(s) 600, 913
MaeIII GTNAC 1 cut(s) 706
MalI GATC 3 cut(s) 252, 552, 574
MboI GATC 3 cut(s) 250, 550, 572
MboII GAAGA 6 cut(s) 18, 30, 221, 338, 481, 752
MflI RGATCY 3 cut(s) 250, 550, 572
MhlI GDGCHC 2 cut(s) 655, 895
MluCI AATT 6 cut(s) 46, 195, 212, 225, 257, 486
MlyI GAGTC 3 cut(s) 459, 637, 849
MmeI TCCRAC 1 cut(s) 289
MseI TTAA 6 cut(s) 198, 255, 377, 507, 722, 828
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 4 cut(s) 130, 340, 570, 888
MvaI CCWGG 4 cut(s) 130, 340, 570, 888
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 6 cut(s) 111, 120, 400, 644, 659, 852
NcoI CCATGG 1 cut(s) 80
NdeII GATC 3 cut(s) 250, 550, 572
NlaIII CATG 5 cut(s) 35, 63, 84, 167, 590
NlaIV GGNNCC 3 cut(s) 180, 351, 652
NspI RCATGY 2 cut(s) 35, 590
PfeI GAWTC 2 cut(s) 160, 243
PkrI GCNGC 5 cut(s) 169, 640, 661, 664, 848
PleI GAGTC 3 cut(s) 458, 636, 848
PpsI GAGTC 3 cut(s) 458, 636, 848
PpuMI RGGWCCY 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 126
Psp6I CCWGG 4 cut(s) 128, 338, 568, 886
PspGI CCWGG 4 cut(s) 128, 338, 568, 886
PspN4I GGNNCC 3 cut(s) 180, 351, 652
PspPI GGNCC 1 cut(s) 126
PspPPI RGGWCCY 1 cut(s) 126
PstI CTGCAG 2 cut(s) 640, 664
PstNI CAGNNNCTG 2 cut(s) 129, 415
PsuI RGATCY 3 cut(s) 250, 550, 572
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
SaqAI TTAA 6 cut(s) 198, 255, 377, 507, 722, 828
SatI GCNGC 5 cut(s) 168, 639, 660, 663, 847
Sau3AI GATC 3 cut(s) 250, 550, 572
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 3 cut(s) 459, 637, 849
ScrFI CCNGG 4 cut(s) 130, 340, 570, 888
SduI GDGCHC 2 cut(s) 655, 895
SexAI ACCWGGT 1 cut(s) 886
SfaNI GCATC 4 cut(s) 114, 344, 423, 682
SfcI CTRYAG 2 cut(s) 636, 660
SinI GGWCC 1 cut(s) 126
Sse9I AATT 6 cut(s) 46, 195, 212, 225, 257, 486
SsiI CCGC 1 cut(s) 168
SspMI CTAG 2 cut(s) 600, 913
StyD4I CCNGG 4 cut(s) 128, 338, 568, 886
StyI CCWWGG 2 cut(s) 80, 445
TaaI ACNGT 2 cut(s) 417, 672
TaqI TCGA 3 cut(s) 117, 300, 626
TasI AATT 6 cut(s) 46, 195, 212, 225, 257, 486
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 160, 243
Tru1I TTAA 6 cut(s) 198, 255, 377, 507, 722, 828
Tru9I TTAA 6 cut(s) 198, 255, 377, 507, 722, 828
TscAI CASTG 2 cut(s) 640, 775
TseI GCWGC 4 cut(s) 638, 659, 662, 846
TspDTI ATGAA 3 cut(s) 152, 492, 549
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 2 cut(s) 640, 775
VneI GTGCAC 1 cut(s) 891
VpaK11BI GGWCC 1 cut(s) 126
XagI CCTNNNNNAGG 1 cut(s) 144
XapI RAATTY 3 cut(s) 212, 225, 257
XbaI TCTAGA 1 cut(s) 599
XceI RCATGY 2 cut(s) 35, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 863
XspI CTAG 2 cut(s) 600, 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.