Rh3CG023000

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002815
Physical Location & Seq
Forward (+)
1571523 .. 1574170
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG023000.1

Sequence Viewer

Length: 1362 bp
ATGAGAAGAGGAGGAGAAGGAGGTTCTTCAGGGAATTACAGAAACCCATGTCTGACTATGCACCAGCCATGGGCTTCTCTGCTCGTATATGGTATCAAACGCATCGAGGGCAGGTCATGGCCTGCTCCCATTAGAGGACGTCTTTGGATTCATGCAGCTGGTAAGGTTCCAGAGGAGGCTACAATCAAAGCAATGGAGGATTTCTATCGGGAAATTTATGCCGTGGATGGAGTCACTGATATCAAATTTCCCCAACACTATCCAGTTTCAAGACTGCTAGGGTGTGTTGAAGTAGTTGGATGTGTTAGACGCGAAGAGTTAGCTGGTTGGGAGATGGTTCCTGAAGGGGTGAGGCTGGAAGCACAAACCGATATGTGTTGGCTTTGCGAGCAGCCACAGAAACTGTTAATTCCGTTTGAGATGCGTGGCTACCAAGGTGTCTATAACTTGGAAAAGAAGATATATGAAGCTGCTATTAGAGGTCTCACCCCGGTTGAAGTTCCTCTCCCAGTTAAATTTCCACTTCCAAATCCACAAGATCCATTTTCCTTGAAGCCAGGGTCTATATCTGCAAACGTTGATCAATCTAGAACATCTGAAGCGGGAAGATCTTCGAGTCTCAATGCAGCTATAGCTGGTGCACGAGCAGCTGCTACACAGTTCTCCAAAAAACCTCAAACTTTCCCAACTACCTCCCTAAATGATGCAGCCAAATCTGTGACAGTTCCAATCAAGAGTGAATCTTTTGAAGGAGGAAGAGCAATGACCGGTAATTTCAGTGAGAGCTCCAACAAAGAGTCAATGACATCAAATAACAAAGATCAAATCAGCAGCCGTAATCAACCTTCTTCTGGAGCTATTAGACCTCATCCTGGTGCACCTTCTAAGATATATGAAGCAGCTATTAGAGGTCTTACCCCTGTTGAAGTTCCCCTTAAAGTCAAATTTCCAGTTCCAAATCCAGAAGATCCATTTTCCTTGAAGCCAGGATTTATATCTGCTAATGTCGATCAATCTAGAACATCTGAAGTGGTAAGATCTTCTAGTCTCAATACAGCCATAGCTGGTGCACAAGCAGCTGATACACAGTTCTCCAAAAAAACTCAAACTTTCCCAACTACCTCCCTAAATAATGCCGCGGAATCTGTGATGACAGTTCCAATCAGGAGTGAGTCTTCTGAAGTGGGTAAAGTGACGTCTGCTAATTTTATTGAGAGTTCTAATAAGAGGTCTATGACATCAAATAACAAAGATCAAATCAGCAGAGGTGATCAACCTTCTTCTGGAGCTTTTAGACCTCATCCTGGTGCACCTTCTAAGTATGTTTATGAACGGAGGAGGCGACGAGATGCCCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

453

Amino Acids

49.54

Weight (kDa)

9.41

Isoelectric Point (pI)

56.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 18 - 106 3.9e-08 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 142, 1199
Acc36I ACCTGC 1 cut(s) 102
AccII CGCG 2 cut(s) 312, 1139
AciI CCGC 3 cut(s) 602, 1137, 1139
AclI AACGTT 1 cut(s) 576
AclWI GGATC 2 cut(s) 533, 962
AcsI RAATTY 4 cut(s) 213, 245, 515, 944
AcuI CTGAAG 5 cut(s) 12, 363, 618, 1047, 1200
AcyI GRCGYC 2 cut(s) 139, 1196
AfiI CCNNNNNNNGG 6 cut(s) 70, 134, 851, 872, 1283, 1304
AgeI ACCGGT 1 cut(s) 767
AgsI TTSAA 7 cut(s) 270, 290, 497, 553, 749, 926, 982
AjnI CCWGG 4 cut(s) 556, 871, 985, 1303
Alw21I GWGCWC 5 cut(s) 643, 788, 880, 1072, 1312
Alw26I GTCTC 3 cut(s) 488, 623, 1052
Alw44I GTGCAC 4 cut(s) 639, 876, 1068, 1308
AlwI GGATC 2 cut(s) 533, 962
AlwNI CAGNNNCTG 1 cut(s) 403
AoxI GGCC 1 cut(s) 119
ApaLI GTGCAC 4 cut(s) 639, 876, 1068, 1308
ApoI RAATTY 4 cut(s) 213, 245, 515, 944
AsiGI ACCGGT 1 cut(s) 767
Asp700I GAANNNNTTC 1 cut(s) 610
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 3 cut(s) 361, 478, 1280
BaeGI GKGCMC 4 cut(s) 643, 880, 1072, 1312
BanII GRGCYC 1 cut(s) 788
BarI GAAGNNNNNNTAC 2 cut(s) 829, 861
BauI CACGAG 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 1167
Bbv12I GWGCWC 5 cut(s) 643, 788, 880, 1072, 1312
BccI CCATC 2 cut(s) 221, 328
BceAI ACGGC 2 cut(s) 206, 819
BciT130I CCWGG 4 cut(s) 558, 873, 987, 1305
BclI TGATCA 2 cut(s) 580, 1270
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 3 cut(s) 488, 623, 1052
BfaI CTAG 4 cut(s) 278, 588, 1017, 1044
BfmI CTRYAG 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 102
BglII AGATCT 2 cut(s) 608, 1037
Bme1390I CCNGG 5 cut(s) 491, 558, 873, 987, 1305
BmiI GGNNCC 2 cut(s) 168, 339
BmrFI CCNGG 5 cut(s) 491, 558, 873, 987, 1305
BmrI ACTGGG 2 cut(s) 503, 1348
BmsI GCATC 4 cut(s) 111, 411, 694, 1339
BmuI ACTGGG 2 cut(s) 503, 1348
BpiI GAAGAC 1 cut(s) 1167
BpmI CTGGAG 2 cut(s) 873, 1305
BpuMI CCSGG 1 cut(s) 491
BsaBI GATNNNNATC 2 cut(s) 204, 994
BsaHI GRCGYC 2 cut(s) 139, 1196
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 6 cut(s) 68, 222, 433, 489, 557, 1137
BsaWI WCCGGW 1 cut(s) 767
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 134, 851, 872, 1283, 1304
Bse118I RCCGGY 1 cut(s) 767
Bse1I ACTGG 4 cut(s) 263, 509, 950, 1354
Bse3DI GCAATG 2 cut(s) 198, 768
Bse8I GATNNNNATC 2 cut(s) 204, 994
BseBI CCWGG 4 cut(s) 558, 873, 987, 1305
BseDI CCNNGG 6 cut(s) 68, 222, 433, 489, 557, 1137
BseGI GGATG 4 cut(s) 232, 305, 868, 1300
BseJI GATNNNNATC 2 cut(s) 204, 994
BseLI CCNNNNNNNGG 6 cut(s) 70, 134, 851, 872, 1283, 1304
BseMI GCAATG 2 cut(s) 198, 768
BseNI ACTGG 4 cut(s) 263, 509, 950, 1354
BseRI GAGGAG 4 cut(s) 24, 27, 188, 1351
BseSI GKGCMC 4 cut(s) 643, 880, 1072, 1312
Bsh1236I CGCG 2 cut(s) 312, 1139
BshFI GGCC 1 cut(s) 121
BshTI ACCGGT 1 cut(s) 767
BsiHKAI GWGCWC 5 cut(s) 643, 788, 880, 1072, 1312
BsiSI CCGG 2 cut(s) 491, 768
BslI CCNNNNNNNGG 6 cut(s) 70, 134, 851, 872, 1283, 1304
BsmAI GTCTC 3 cut(s) 488, 623, 1052
BsnI GGCC 1 cut(s) 121
Bso31I GGTCTC 1 cut(s) 488
Bsp1286I GDGCHC 5 cut(s) 643, 788, 880, 1072, 1312
Bsp143I GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
Bsp19I CCATGG 1 cut(s) 68
BspACI CCGC 3 cut(s) 602, 1137, 1139
BspANI GGCC 1 cut(s) 121
BspFNI CGCG 2 cut(s) 312, 1139
BspLI GGNNCC 2 cut(s) 168, 339
BspMI ACCTGC 1 cut(s) 102
BspPI GGATC 2 cut(s) 533, 962
BspQI GCTCTTC 1 cut(s) 751
BspTNI GGTCTC 1 cut(s) 488
BsrDI GCAATG 2 cut(s) 198, 768
BsrFI RCCGGY 1 cut(s) 767
BsrI ACTGG 4 cut(s) 263, 509, 950, 1354
BssAI RCCGGY 1 cut(s) 767
BssECI CCNNGG 6 cut(s) 68, 222, 433, 489, 557, 1137
BssMI GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
BssNI GRCGYC 2 cut(s) 139, 1196
BssSI CACGAG 1 cut(s) 642
BssT1I CCWWGG 2 cut(s) 68, 433
Bst2BI CACGAG 1 cut(s) 642
Bst2UI CCWGG 4 cut(s) 558, 873, 987, 1305
Bst4CI ACNGT 5 cut(s) 405, 660, 724, 1089, 1156
Bst6I CTCTTC 2 cut(s) 309, 751
BstACI GRCGYC 2 cut(s) 139, 1196
BstC8I GCNNGC 2 cut(s) 123, 389
BstDEI CTNAG 2 cut(s) 885, 1317
BstDSI CCRYGG 3 cut(s) 68, 222, 1137
BstF5I GGATG 4 cut(s) 232, 305, 868, 1300
BstFNI CGCG 2 cut(s) 312, 1139
BstKTI GATC 9 cut(s) 541, 583, 611, 823, 970, 1012, 1040, 1255, 1273
BstMAI GTCTC 3 cut(s) 488, 623, 1052
BstMBI GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
BstMWI GCNNNNNNNGC 5 cut(s) 108, 388, 632, 647, 1076
BstNI CCWGG 4 cut(s) 558, 873, 987, 1305
BstSCI CCNGG 5 cut(s) 489, 556, 871, 985, 1303
BstSFI CTRYAG 1 cut(s) 630
BstSLI GKGCMC 4 cut(s) 643, 880, 1072, 1312
BstUI CGCG 2 cut(s) 312, 1139
BstV2I GAAGAC 1 cut(s) 1167
BstX2I RGATCY 4 cut(s) 538, 608, 967, 1037
BstYI RGATCY 4 cut(s) 538, 608, 967, 1037
BsuRI GGCC 1 cut(s) 121
BtgI CCRYGG 3 cut(s) 68, 222, 1137
BtsCI GGATG 4 cut(s) 232, 305, 868, 1300
BtsIMutI CAGTG 2 cut(s) 234, 784
BveI ACCTGC 1 cut(s) 102
Cac8I GCNNGC 2 cut(s) 123, 389
CaiI CAGNNNCTG 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 767
Cfr42I CCGCGG 1 cut(s) 1140
CseI GACGC 1 cut(s) 318
CspAI ACCGGT 1 cut(s) 767
CviAII CATG 4 cut(s) 48, 69, 117, 152
DdeI CTNAG 2 cut(s) 885, 1317
DpnI GATC 9 cut(s) 540, 582, 610, 822, 969, 1011, 1039, 1254, 1272
DpnII GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
Eam1104I CTCTTC 2 cut(s) 309, 751
EarI CTCTTC 2 cut(s) 309, 751
Ecl136II GAGCTC 1 cut(s) 786
Eco130I CCWWGG 2 cut(s) 68, 433
Eco24I GRGCYC 1 cut(s) 788
Eco31I GGTCTC 1 cut(s) 488
Eco32I GATATC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 786
Eco57I CTGAAG 5 cut(s) 12, 363, 618, 1047, 1200
EcoICRI GAGCTC 1 cut(s) 786
EcoRII CCWGG 4 cut(s) 556, 871, 985, 1303
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 2 cut(s) 68, 433
EcoT38I GRGCYC 1 cut(s) 788
ErhI CCWWGG 2 cut(s) 68, 433
FaeI CATG 4 cut(s) 51, 72, 120, 155
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FatI CATG 4 cut(s) 47, 68, 116, 151
FauI CCCGC 1 cut(s) 595
FbaI TGATCA 2 cut(s) 580, 1270
FokI GGATG 4 cut(s) 239, 312, 855, 1287
FriOI GRGCYC 1 cut(s) 788
FspBI CTAG 4 cut(s) 278, 588, 1017, 1044
GsuI CTGGAG 2 cut(s) 873, 1305
HaeIII GGCC 1 cut(s) 121
HapII CCGG 2 cut(s) 491, 768
HgaI GACGC 1 cut(s) 318
Hin1I GRCGYC 2 cut(s) 139, 1196
Hin1II CATG 4 cut(s) 51, 72, 120, 155
HinfI GANTC 7 cut(s) 148, 231, 616, 740, 797, 1142, 1172
HpaII CCGG 2 cut(s) 491, 768
HphI GGTGA 3 cut(s) 361, 478, 1280
Hpy166II GTNNAC 4 cut(s) 641, 878, 1070, 1310
Hpy188I TCNGA 4 cut(s) 54, 598, 1027, 1180
Hpy8I GTNNAC 4 cut(s) 641, 878, 1070, 1310
Hpy99I CGWCG 1 cut(s) 1347
HpyAV CCTTC 7 cut(s) 11, 338, 743, 855, 891, 1287, 1323
HpyCH4III ACNGT 5 cut(s) 405, 660, 724, 1089, 1156
HpyCH4IV ACGT 3 cut(s) 139, 576, 1196
HpyCH4V TGCA 9 cut(s) 61, 155, 572, 626, 641, 707, 878, 1070, 1310
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 388, 632, 647, 1076
HpyF3I CTNAG 2 cut(s) 885, 1317
HpySE526I ACGT 3 cut(s) 139, 576, 1196
Hsp92I GRCGYC 2 cut(s) 139, 1196
Hsp92II CATG 4 cut(s) 51, 72, 120, 155
Ksp22I TGATCA 2 cut(s) 580, 1270
KspI CCGCGG 1 cut(s) 1140
Kzo9I GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
LguI GCTCTTC 1 cut(s) 751
LmnI GCTCC 4 cut(s) 130, 791, 854, 1286
LweI GCATC 4 cut(s) 111, 411, 694, 1339
MaeI CTAG 4 cut(s) 278, 588, 1017, 1044
MaeII ACGT 3 cut(s) 139, 576, 1196
MaeIII GTNAC 3 cut(s) 232, 718, 1192
MalI GATC 9 cut(s) 540, 582, 610, 822, 969, 1011, 1039, 1254, 1272
MboI GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
MflI RGATCY 4 cut(s) 538, 608, 967, 1037
MhlI GDGCHC 5 cut(s) 643, 788, 880, 1072, 1312
MluCI AATT 8 cut(s) 34, 213, 245, 408, 515, 772, 944, 1204
MlyI GAGTC 4 cut(s) 240, 625, 806, 1181
MmeI TCCRAC 2 cut(s) 277, 813
MroXI GAANNNNTTC 1 cut(s) 610
MseI TTAA 3 cut(s) 407, 513, 936
MslI CAYNNNNRTG 2 cut(s) 873, 1305
MspA1I CMGCKG 4 cut(s) 158, 650, 1079, 1139
MspI CCGG 2 cut(s) 491, 768
MspR9I CCNGG 5 cut(s) 491, 558, 873, 987, 1305
MvaI CCWGG 4 cut(s) 558, 873, 987, 1305
MvnI CGCG 2 cut(s) 312, 1139
MwoI GCNNNNNNNGC 5 cut(s) 108, 388, 632, 647, 1076
NciI CCSGG 1 cut(s) 491
NcoI CCATGG 1 cut(s) 68
NdeII GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
NlaIII CATG 4 cut(s) 51, 72, 120, 155
NlaIV GGNNCC 2 cut(s) 168, 339
NmuCI GTSAC 3 cut(s) 232, 718, 1192
PciSI GCTCTTC 1 cut(s) 751
PcsI WCGNNNNNNNCGW 1 cut(s) 1339
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 3 cut(s) 148, 740, 1142
PinAI ACCGGT 1 cut(s) 767
PleI GAGTC 4 cut(s) 239, 624, 805, 1180
PpsI GAGTC 4 cut(s) 239, 624, 805, 1180
Psp124BI GAGCTC 1 cut(s) 788
Psp1406I AACGTT 1 cut(s) 576
Psp6I CCWGG 4 cut(s) 556, 871, 985, 1303
PspGI CCWGG 4 cut(s) 556, 871, 985, 1303
PspN4I GGNNCC 2 cut(s) 168, 339
PstNI CAGNNNCTG 1 cut(s) 403
PsuI RGATCY 4 cut(s) 538, 608, 967, 1037
PvuII CAGCTG 3 cut(s) 158, 650, 1079
RseI CAYNNNNRTG 2 cut(s) 873, 1305
SacI GAGCTC 1 cut(s) 788
SacII CCGCGG 1 cut(s) 1140
SapI GCTCTTC 1 cut(s) 751
SaqAI TTAA 3 cut(s) 407, 513, 936
Sau3AI GATC 9 cut(s) 538, 580, 608, 820, 967, 1009, 1037, 1252, 1270
SchI GAGTC 4 cut(s) 240, 625, 806, 1181
ScrFI CCNGG 5 cut(s) 491, 558, 873, 987, 1305
SduI GDGCHC 5 cut(s) 643, 788, 880, 1072, 1312
SfaNI GCATC 4 cut(s) 111, 411, 694, 1339
SfcI CTRYAG 1 cut(s) 630
Sfr303I CCGCGG 1 cut(s) 1140
SgrBI CCGCGG 1 cut(s) 1140
SmiMI CAYNNNNRTG 2 cut(s) 873, 1305
Sse9I AATT 8 cut(s) 34, 213, 245, 408, 515, 772, 944, 1204
SsiI CCGC 3 cut(s) 602, 1137, 1139
SspMI CTAG 4 cut(s) 278, 588, 1017, 1044
SstI GAGCTC 1 cut(s) 788
StyD4I CCNGG 5 cut(s) 489, 556, 871, 985, 1303
StyI CCWWGG 2 cut(s) 68, 433
TaaI ACNGT 5 cut(s) 405, 660, 724, 1089, 1156
TaiI ACGT 3 cut(s) 142, 579, 1199
TaqI TCGA 3 cut(s) 105, 614, 1008
TasI AATT 8 cut(s) 34, 213, 245, 408, 515, 772, 944, 1204
TauI GCSGC 1 cut(s) 1139
TfiI GAWTC 3 cut(s) 148, 740, 1142
Tru1I TTAA 3 cut(s) 407, 513, 936
Tru9I TTAA 3 cut(s) 407, 513, 936
TscAI CASTG 2 cut(s) 241, 784
TseFI GTSAC 3 cut(s) 232, 718, 1192
Tsp45I GTSAC 3 cut(s) 232, 718, 1192
TspDTI ATGAA 4 cut(s) 140, 480, 909, 1344
TspGWI ACGGA 2 cut(s) 402, 1348
TspRI CASTG 2 cut(s) 241, 784
VneI GTGCAC 4 cut(s) 639, 876, 1068, 1308
XapI RAATTY 4 cut(s) 213, 245, 515, 944
XbaI TCTAGA 2 cut(s) 587, 1016
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 4 cut(s) 278, 588, 1017, 1044
ZraI GACGTC 2 cut(s) 140, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.