RLG00000025772

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002815
Physical Location & Seq
Reverse (-)
48185642 .. 48188271
2630 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025772

Sequence Viewer

Length: 1344 bp
ATGAGAAGAGGAGGAGGAGGTTCTTCTGGGAATTACAGAAACCCATGTCTGACTATGCACCAGCCATGGGCTTCTCTGCTCGTATATGGTATCAAACGCATCGAGGGCAGGTCATGGCCTGCTCCCATTAGAGGACGTCTTTGGATTCATGCTGCTGGTAAGGTTCCAGAGGAGGCTACAATCAAAGCAATGGAGGATTTCTATCGGGAAATTTATGCGGTGGATGGAGTCACTGATATCAAATTTCCCCAACACTATCCAGTTTCAAGACTGCTAGGGTGTGTTGAAGTAGTTGGATGTGTTAGACGCGAAGAGTTAGCTGGTTGGGAGATGGTTCCTGAAGGGGTGAGGCTGGAAGCACAAACTGATATGTGTTGGCTTTGCGAGCAGCCACAGAAACTGTTAATTCCGTTTGAGATGCGTGGCTACCAAGGTGTCTATAACTTGGAAAAGAAGATATATGAAGCTGCTATTAGAGGTCTCACCCCGGTTGAAGTTCCTCTCCCAGTTAAATTTCCACTTCCAAATCCACAAGATCCATTTTCCTTGAAGCTGGGCTCTATATCTGCAAACGTTGATCAATCAAGAACATCTGAAGCGGGAAGATCTTCGAGTCTCAATGCAGCTATAGCTGGTGCACGAGCAGCTGCTACACAGTTCTCCAAAAAACCTCAAACTTTCCCAACTACCTCCCTAAATGATGCAGCCAAATCTGTGACAAGTCCAATCAAGAGTGAATCTTTTGAAGGAGGTAGAGCAATGACCGGTAATTTCAGTGAGAGCTCCAATAAAGAGTCTATGACATCAAATAATAAAGATCAAATCAGCAGCCGTAATCAACCTTCTTCTGGAGCTATTAGACCTGATCCTGGTGCACCTTCTAAGATATATGAAGCGGCTATTAAAGGTCTTACCCCTGTTGAAGTTCCCCTTAAAGTCAAATTTCCAGTTCCAAATCCAGAAGATCCATTTTCCTTGAAGCCAGGATTTATATCTGCTAATGTCGATCAATCTAGAACATCTGAAGTGGTAAGATCTTCTAGTCTCAATACAGCCATAGCTGGTGCACAAGCAGCTGATACACAGTTCTCCAAAAAGGCTCAAACTTTCCCAACTACCTCCCTAAATGATGCCGCGGAATCTGTGATGACAGTTCCAATCAGGAGTGAGTCTTCTGAAGTGGGTAAAGTGACGTCCGCTAATTTTATTGAGAGTTCTAATAAGAGGTCTATGACATCAAATAACAAAGATCAAATCAGCAGAGGTGATCAACCTTCTTCTGGAGCTTTTAGACCTTATCCTGGTGCACCTTCTAAGTATGTTTATGAACGGAGGAGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

448

Amino Acids

48.72

Weight (kDa)

9.25

Isoelectric Point (pI)

56.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 17 - 105 3.9e-08 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 139, 1196
Acc36I ACCTGC 1 cut(s) 99
AccII CGCG 2 cut(s) 309, 1136
AciI CCGC 6 cut(s) 218, 599, 896, 1134, 1136, 1197
AclI AACGTT 1 cut(s) 573
AclWI GGATC 3 cut(s) 530, 860, 959
AcsI RAATTY 4 cut(s) 210, 242, 512, 941
AcuI CTGAAG 4 cut(s) 360, 615, 1044, 1197
AcyI GRCGYC 2 cut(s) 136, 1193
AfiI CCNNNNNNNGG 6 cut(s) 67, 131, 848, 869, 1280, 1301
AgeI ACCGGT 1 cut(s) 764
AgsI TTSAA 7 cut(s) 267, 287, 494, 550, 746, 923, 979
AjnI CCWGG 3 cut(s) 868, 982, 1300
Alw21I GWGCWC 5 cut(s) 640, 785, 877, 1069, 1309
Alw26I GTCTC 3 cut(s) 485, 620, 1049
Alw44I GTGCAC 4 cut(s) 636, 873, 1065, 1305
AlwI GGATC 3 cut(s) 530, 860, 959
AlwNI CAGNNNCTG 1 cut(s) 400
AoxI GGCC 1 cut(s) 116
ApaLI GTGCAC 4 cut(s) 636, 873, 1065, 1305
ApeKI GCWGC 9 cut(s) 152, 388, 467, 623, 644, 647, 704, 828, 1073
ApoI RAATTY 4 cut(s) 210, 242, 512, 941
AsiGI ACCGGT 1 cut(s) 764
Asp700I GAANNNNTTC 1 cut(s) 607
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 3 cut(s) 358, 475, 1277
BaeGI GKGCMC 4 cut(s) 640, 877, 1069, 1309
BanII GRGCYC 2 cut(s) 560, 785
BarI GAAGNNNNNNTAC 2 cut(s) 826, 858
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 1 cut(s) 1164
Bbv12I GWGCWC 5 cut(s) 640, 785, 877, 1069, 1309
BbvI GCAGC 9 cut(s) 139, 400, 454, 634, 635, 656, 716, 840, 1085
BccI CCATC 2 cut(s) 218, 325
BceAI ACGGC 1 cut(s) 816
BciT130I CCWGG 3 cut(s) 870, 984, 1302
BclI TGATCA 2 cut(s) 577, 1267
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 3 cut(s) 485, 620, 1049
BfaI CTAG 3 cut(s) 275, 1014, 1041
BfmI CTRYAG 1 cut(s) 627
BfuAI ACCTGC 1 cut(s) 99
BglII AGATCT 2 cut(s) 605, 1034
Bme1390I CCNGG 4 cut(s) 488, 870, 984, 1302
BmiI GGNNCC 2 cut(s) 165, 336
BmrFI CCNGG 4 cut(s) 488, 870, 984, 1302
BmrI ACTGGG 1 cut(s) 500
BmsI GCATC 4 cut(s) 108, 408, 691, 1120
BmuI ACTGGG 1 cut(s) 500
BpiI GAAGAC 1 cut(s) 1164
BpmI CTGGAG 2 cut(s) 870, 1302
BpuMI CCSGG 1 cut(s) 488
BsaBI GATNNNNATC 2 cut(s) 201, 991
BsaHI GRCGYC 2 cut(s) 136, 1193
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 4 cut(s) 65, 430, 486, 1134
BsaWI WCCGGW 1 cut(s) 764
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 131, 848, 869, 1280, 1301
Bse118I RCCGGY 1 cut(s) 764
Bse1I ACTGG 3 cut(s) 260, 506, 947
Bse3DI GCAATG 2 cut(s) 195, 765
Bse8I GATNNNNATC 2 cut(s) 201, 991
BseBI CCWGG 3 cut(s) 870, 984, 1302
BseDI CCNNGG 4 cut(s) 65, 430, 486, 1134
BseGI GGATG 2 cut(s) 229, 302
BseJI GATNNNNATC 2 cut(s) 201, 991
BseLI CCNNNNNNNGG 6 cut(s) 67, 131, 848, 869, 1280, 1301
BseMI GCAATG 2 cut(s) 195, 765
BseNI ACTGG 3 cut(s) 260, 506, 947
BseRI GAGGAG 4 cut(s) 24, 27, 30, 185
BseSI GKGCMC 4 cut(s) 640, 877, 1069, 1309
BseXI GCAGC 9 cut(s) 139, 400, 454, 634, 635, 656, 716, 840, 1085
BseYI CCCAGC 1 cut(s) 553
Bsh1236I CGCG 2 cut(s) 309, 1136
BshFI GGCC 1 cut(s) 118
BshTI ACCGGT 1 cut(s) 764
BsiHKAI GWGCWC 5 cut(s) 640, 785, 877, 1069, 1309
BsiSI CCGG 2 cut(s) 488, 765
BslI CCNNNNNNNGG 6 cut(s) 67, 131, 848, 869, 1280, 1301
BsmAI GTCTC 3 cut(s) 485, 620, 1049
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 485
Bsp1286I GDGCHC 6 cut(s) 560, 640, 785, 877, 1069, 1309
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 6 cut(s) 218, 599, 896, 1134, 1136, 1197
BspANI GGCC 1 cut(s) 118
BspFNI CGCG 2 cut(s) 309, 1136
BspLI GGNNCC 2 cut(s) 165, 336
BspMI ACCTGC 1 cut(s) 99
BspPI GGATC 3 cut(s) 530, 860, 959
BspTNI GGTCTC 1 cut(s) 485
BsrDI GCAATG 2 cut(s) 195, 765
BsrFI RCCGGY 1 cut(s) 764
BsrI ACTGG 3 cut(s) 260, 506, 947
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 4 cut(s) 65, 430, 486, 1134
BssNI GRCGYC 2 cut(s) 136, 1193
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 2 cut(s) 65, 430
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 3 cut(s) 870, 984, 1302
Bst4CI ACNGT 4 cut(s) 402, 657, 1086, 1153
Bst6I CTCTTC 1 cut(s) 306
BstACI GRCGYC 2 cut(s) 136, 1193
BstC8I GCNNGC 2 cut(s) 120, 386
BstDEI CTNAG 2 cut(s) 882, 1314
BstDSI CCRYGG 2 cut(s) 65, 1134
BstF5I GGATG 2 cut(s) 229, 302
BstFNI CGCG 2 cut(s) 309, 1136
BstMAI GTCTC 3 cut(s) 485, 620, 1049
BstMWI GCNNNNNNNGC 5 cut(s) 105, 385, 629, 644, 1073
BstNI CCWGG 3 cut(s) 870, 984, 1302
BstSCI CCNGG 4 cut(s) 486, 868, 982, 1300
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 4 cut(s) 640, 877, 1069, 1309
BstUI CGCG 2 cut(s) 309, 1136
BstV1I GCAGC 9 cut(s) 139, 400, 454, 634, 635, 656, 716, 840, 1085
BstV2I GAAGAC 1 cut(s) 1164
BstX2I RGATCY 4 cut(s) 535, 605, 964, 1034
BstYI RGATCY 4 cut(s) 535, 605, 964, 1034
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 2 cut(s) 65, 1134
BtsCI GGATG 2 cut(s) 229, 302
BtsIMutI CAGTG 2 cut(s) 231, 781
BveI ACCTGC 1 cut(s) 99
Cac8I GCNNGC 2 cut(s) 120, 386
CaiI CAGNNNCTG 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 764
Cfr42I CCGCGG 1 cut(s) 1137
CseI GACGC 1 cut(s) 315
CspAI ACCGGT 1 cut(s) 764
CviAII CATG 4 cut(s) 45, 66, 114, 149
DdeI CTNAG 2 cut(s) 882, 1314
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Ecl136II GAGCTC 1 cut(s) 783
Eco130I CCWWGG 2 cut(s) 65, 430
Eco24I GRGCYC 2 cut(s) 560, 785
Eco31I GGTCTC 1 cut(s) 485
Eco32I GATATC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 783
Eco57I CTGAAG 4 cut(s) 360, 615, 1044, 1197
EcoICRI GAGCTC 1 cut(s) 783
EcoRII CCWGG 3 cut(s) 868, 982, 1300
EcoRV GATATC 1 cut(s) 238
EcoT14I CCWWGG 2 cut(s) 65, 430
EcoT38I GRGCYC 2 cut(s) 560, 785
ErhI CCWWGG 2 cut(s) 65, 430
FaeI CATG 4 cut(s) 48, 69, 117, 152
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FatI CATG 4 cut(s) 44, 65, 113, 148
FauI CCCGC 1 cut(s) 592
FbaI TGATCA 2 cut(s) 577, 1267
FokI GGATG 2 cut(s) 236, 309
FriOI GRGCYC 2 cut(s) 560, 785
FspBI CTAG 3 cut(s) 275, 1014, 1041
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 2 cut(s) 870, 1302
HaeIII GGCC 1 cut(s) 118
HapII CCGG 2 cut(s) 488, 765
HgaI GACGC 1 cut(s) 315
Hin1I GRCGYC 2 cut(s) 136, 1193
Hin1II CATG 4 cut(s) 48, 69, 117, 152
HinfI GANTC 7 cut(s) 145, 228, 613, 737, 794, 1139, 1169
HpaII CCGG 2 cut(s) 488, 765
HphI GGTGA 3 cut(s) 358, 475, 1277
Hpy166II GTNNAC 4 cut(s) 638, 875, 1067, 1307
Hpy188I TCNGA 4 cut(s) 51, 595, 1024, 1177
Hpy8I GTNNAC 4 cut(s) 638, 875, 1067, 1307
HpyAV CCTTC 6 cut(s) 335, 740, 852, 888, 1284, 1320
HpyCH4III ACNGT 4 cut(s) 402, 657, 1086, 1153
HpyCH4IV ACGT 3 cut(s) 136, 573, 1193
HpyCH4V TGCA 8 cut(s) 58, 569, 623, 638, 704, 875, 1067, 1307
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 385, 629, 644, 1073
HpyF3I CTNAG 2 cut(s) 882, 1314
HpySE526I ACGT 3 cut(s) 136, 573, 1193
Hsp92I GRCGYC 2 cut(s) 136, 1193
Hsp92II CATG 4 cut(s) 48, 69, 117, 152
Ksp22I TGATCA 2 cut(s) 577, 1267
KspI CCGCGG 1 cut(s) 1137
LmnI GCTCC 4 cut(s) 127, 788, 851, 1283
Lsp1109I GCAGC 9 cut(s) 139, 400, 454, 634, 635, 656, 716, 840, 1085
LweI GCATC 4 cut(s) 108, 408, 691, 1120
MaeI CTAG 3 cut(s) 275, 1014, 1041
MaeII ACGT 3 cut(s) 136, 573, 1193
MaeIII GTNAC 3 cut(s) 229, 715, 1189
MflI RGATCY 4 cut(s) 535, 605, 964, 1034
MhlI GDGCHC 6 cut(s) 560, 640, 785, 877, 1069, 1309
MluCI AATT 8 cut(s) 31, 210, 242, 405, 512, 769, 941, 1201
MlyI GAGTC 4 cut(s) 237, 622, 803, 1178
MmeI TCCRAC 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 607
MseI TTAA 4 cut(s) 404, 510, 903, 933
MspA1I CMGCKG 3 cut(s) 647, 1076, 1136
MspI CCGG 2 cut(s) 488, 765
MspR9I CCNGG 4 cut(s) 488, 870, 984, 1302
MvaI CCWGG 3 cut(s) 870, 984, 1302
MvnI CGCG 2 cut(s) 309, 1136
MwoI GCNNNNNNNGC 5 cut(s) 105, 385, 629, 644, 1073
NciI CCSGG 1 cut(s) 488
NcoI CCATGG 1 cut(s) 65
NlaIII CATG 4 cut(s) 48, 69, 117, 152
NlaIV GGNNCC 2 cut(s) 165, 336
NmuCI GTSAC 3 cut(s) 229, 715, 1189
PdmI GAANNNNTTC 1 cut(s) 607
PfeI GAWTC 3 cut(s) 145, 737, 1139
PinAI ACCGGT 1 cut(s) 764
PleI GAGTC 4 cut(s) 236, 621, 802, 1177
PpsI GAGTC 4 cut(s) 236, 621, 802, 1177
Psp124BI GAGCTC 1 cut(s) 785
Psp1406I AACGTT 1 cut(s) 573
Psp6I CCWGG 3 cut(s) 868, 982, 1300
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 3 cut(s) 868, 982, 1300
PspN4I GGNNCC 2 cut(s) 165, 336
PstNI CAGNNNCTG 1 cut(s) 400
PsuI RGATCY 4 cut(s) 535, 605, 964, 1034
PvuII CAGCTG 2 cut(s) 647, 1076
SacI GAGCTC 1 cut(s) 785
SacII CCGCGG 1 cut(s) 1137
SaqAI TTAA 4 cut(s) 404, 510, 903, 933
SchI GAGTC 4 cut(s) 237, 622, 803, 1178
ScrFI CCNGG 4 cut(s) 488, 870, 984, 1302
SduI GDGCHC 6 cut(s) 560, 640, 785, 877, 1069, 1309
SfaNI GCATC 4 cut(s) 108, 408, 691, 1120
SfcI CTRYAG 1 cut(s) 627
Sfr303I CCGCGG 1 cut(s) 1137
SgrBI CCGCGG 1 cut(s) 1137
Sse9I AATT 8 cut(s) 31, 210, 242, 405, 512, 769, 941, 1201
SsiI CCGC 6 cut(s) 218, 599, 896, 1134, 1136, 1197
SspMI CTAG 3 cut(s) 275, 1014, 1041
SstI GAGCTC 1 cut(s) 785
StyD4I CCNGG 4 cut(s) 486, 868, 982, 1300
StyI CCWWGG 2 cut(s) 65, 430
TaaI ACNGT 4 cut(s) 402, 657, 1086, 1153
TaiI ACGT 3 cut(s) 139, 576, 1196
TaqI TCGA 3 cut(s) 102, 611, 1005
TasI AATT 8 cut(s) 31, 210, 242, 405, 512, 769, 941, 1201
TauI GCSGC 2 cut(s) 899, 1136
TfiI GAWTC 3 cut(s) 145, 737, 1139
Tru1I TTAA 4 cut(s) 404, 510, 903, 933
Tru9I TTAA 4 cut(s) 404, 510, 903, 933
TscAI CASTG 2 cut(s) 238, 781
TseFI GTSAC 3 cut(s) 229, 715, 1189
TseI GCWGC 9 cut(s) 152, 388, 467, 623, 644, 647, 704, 828, 1073
Tsp45I GTSAC 3 cut(s) 229, 715, 1189
TspDTI ATGAA 4 cut(s) 137, 477, 906, 1341
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 2 cut(s) 238, 781
VneI GTGCAC 4 cut(s) 636, 873, 1065, 1305
XapI RAATTY 4 cut(s) 210, 242, 512, 941
XbaI TCTAGA 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 607
XspI CTAG 3 cut(s) 275, 1014, 1041
ZraI GACGTC 2 cut(s) 137, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.