MD04G1229000.v1.1

ASCH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002815
Physical Location & Seq
Reverse (-)
30922204 .. 30924217
2014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1229000.v1.1.491

Sequence Viewer

Length: 972 bp
ATGAGAAGAGGAGGAGGAGGAGGAGGAGGACATTCTTCTTCTGGGAATTACAGAAACCCATGTCTGACCATGCACCAACCATGGGCTTCTTTGCTGGTTTATGGCATCAAGCACGTCGAGGGCAGGTCCTGGCCTTCTCCTATCCGAGGCCGTCTCTGGATTCATGCCGCTGGTAAGGTTCCTGATGAGGCTACAATCAAGGCAATGGAGGAATTCTACAGAGAAATTTATGCAGTTGATGGAATCACTGATCTTAAATTTCCGGAACATTATCCCGTTTCCAGACATTTAGGTATTCTAAGTTTGGTGAGGCTGGAAGCACAAACTGATATGTGTTGGCTTTGTGAGCAGCCACAGAAACTGTTAGTTCCATTTGAGATGTGCGTGGCTACCAAGGTGTCTACAACTTGGAAAAGAAGGTGCATATTGTGCATAAGAATTTACTCTCTCTCTCTCTCTCTCTCCCCTACTGTTTTTGGTGGACCTAACTTACTCATTTGCCTGTCTCAGATATATGAAGGTGCAATTAGAGGTCTTTCCCCGGTTAATAGTCCCCTTCCAGTAAGATTTCCACTTCCAAATCCACAAGATCCATTCTCGCTGAAGCCAGGGTCTATCTCTGTGCATGTCCCTGTATCTAGAACATCTGAAGTTGTGATATCTTCGAGTCCCACTGCAGCCATAGCTGGTGCGCGTGCTGCAGCTACACAGTTCTCGAAGAAAGTTCAAGATTTTGAAACGACTGCCCAAAATAATGCATCGGAATCTGTAACGACCCTTCCATTAAAGGGTGAATCTGTTGAAAAAGATACAACACCATCTGCTAAACTTAGTGAGACTTCTAACAAGGGAGAATTGACATCAAATAACAAGGAGCAAATAAGTCCCGTTAAAGACAAGGGAAGCTGCAGCCTCAGCCAACCTTATTCTGGAGCTTTGAGACCTCGACCTGGTGCACCTTCGAAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ASCH

Protein Analysis

324

Amino Acids

35.04

Weight (kDa)

9.24

Isoelectric Point (pI)

54.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ASCH PF04266 22 - 57 5.8e-06 ASCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 401
AccII CGCG 1 cut(s) 694
AccIII TCCGGA 1 cut(s) 262
AciI CCGC 1 cut(s) 168
AclWI GGATC 1 cut(s) 584
AcsI RAATTY 4 cut(s) 212, 225, 257, 438
AcuI CTGAAG 2 cut(s) 623, 669
AfiI CCNNNNNNNGG 5 cut(s) 82, 146, 788, 929, 950
AgsI TTSAA 3 cut(s) 728, 737, 803
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 3 cut(s) 128, 607, 949
AluBI AGCT 4 cut(s) 686, 704, 906, 935
AluI AGCT 4 cut(s) 686, 704, 906, 935
Alw21I GWGCWC 1 cut(s) 958
Alw26I GTCTC 4 cut(s) 158, 510, 830, 934
Alw44I GTGCAC 1 cut(s) 954
AlwI GGATC 1 cut(s) 584
AlwNI CAGNNNCTG 2 cut(s) 129, 361
Aor13HI TCCGGA 1 cut(s) 262
AoxI GGCC 2 cut(s) 131, 148
ApaLI GTGCAC 1 cut(s) 954
ApeKI GCWGC 6 cut(s) 349, 677, 698, 701, 906, 909
ApoI RAATTY 4 cut(s) 212, 225, 257, 438
AspLEI GCGC 1 cut(s) 694
AspS9I GGNCC 2 cut(s) 126, 482
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 2 cut(s) 319, 803
AsuII TTCGAA 1 cut(s) 962
AvaII GGWCC 2 cut(s) 126, 482
BaeGI GKGCMC 1 cut(s) 958
BarI GAAGNNNNNNTAC 2 cut(s) 762, 794
Bbv12I GWGCWC 1 cut(s) 958
BbvCI CCTCAGC 1 cut(s) 914
BbvI GCAGC 6 cut(s) 361, 685, 689, 713, 893, 921
BccI CCATC 2 cut(s) 233, 826
BceAI ACGGC 1 cut(s) 135
BciT130I CCWGG 3 cut(s) 130, 609, 951
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 4 cut(s) 158, 510, 830, 934
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 4 cut(s) 217, 675, 699, 907
BfuAI ACCTGC 1 cut(s) 114
BisI GCNGC 7 cut(s) 168, 350, 678, 699, 702, 907, 910
BlsI GCNGC 7 cut(s) 169, 351, 679, 700, 703, 908, 911
Bme1390I CCNGG 4 cut(s) 130, 542, 609, 951
Bme18I GGWCC 2 cut(s) 126, 482
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 2 cut(s) 126, 482
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 4 cut(s) 130, 542, 609, 951
BmsI GCATC 2 cut(s) 114, 767
BplI GAGNNNNNCTC 2 cut(s) 138, 170
BpmI CTGGAG 1 cut(s) 951
Bpu10I CCTNAGC 1 cut(s) 914
Bpu14I TTCGAA 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 542
BsaI GGTCTC 1 cut(s) 934
BsaJI CCNNGG 5 cut(s) 80, 145, 393, 540, 608
BsaWI WCCGGW 1 cut(s) 262
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 146, 788, 929, 950
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 1 cut(s) 210
BseAI TCCGGA 1 cut(s) 262
BseBI CCWGG 3 cut(s) 130, 609, 951
BseDI CCNNGG 5 cut(s) 80, 145, 393, 540, 608
BseLI CCNNNNNNNGG 5 cut(s) 82, 146, 788, 929, 950
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 2 cut(s) 521, 928
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 6 cut(s) 24, 27, 30, 33, 36, 39
BseSI GKGCMC 1 cut(s) 958
BseXI GCAGC 6 cut(s) 361, 685, 689, 713, 893, 921
Bsh1236I CGCG 1 cut(s) 694
BshFI GGCC 2 cut(s) 133, 150
BsiHKAI GWGCWC 1 cut(s) 958
BsiSI CCGG 2 cut(s) 263, 542
BslFI GGGAC 4 cut(s) 537, 614, 654, 870
BslI CCNNNNNNNGG 5 cut(s) 82, 146, 788, 929, 950
BsmAI GTCTC 4 cut(s) 158, 510, 830, 934
BsmBI CGTCTC 1 cut(s) 158
BsmFI GGGAC 4 cut(s) 537, 614, 654, 870
BsnI GGCC 2 cut(s) 133, 150
Bso31I GGTCTC 1 cut(s) 934
Bsp119I TTCGAA 1 cut(s) 962
Bsp1286I GDGCHC 1 cut(s) 958
Bsp13I TCCGGA 1 cut(s) 262
Bsp143I GATC 2 cut(s) 250, 589
Bsp19I CCATGG 1 cut(s) 80
BspACI CCGC 1 cut(s) 168
BspANI GGCC 2 cut(s) 133, 150
BspCNI CTCAG 2 cut(s) 520, 927
BspEI TCCGGA 1 cut(s) 262
BspFNI CGCG 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 180
BspMAI CTGCAG 3 cut(s) 679, 703, 911
BspMI ACCTGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 584
BspT104I TTCGAA 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 934
BsrDI GCAATG 1 cut(s) 210
BsrI ACTGG 1 cut(s) 560
BssECI CCNNGG 5 cut(s) 80, 145, 393, 540, 608
BssMI GATC 2 cut(s) 250, 589
BssT1I CCWWGG 2 cut(s) 80, 393
Bst2UI CCWGG 3 cut(s) 130, 609, 951
Bst4CI ACNGT 3 cut(s) 363, 472, 711
BstAPI GCANNNNNTGC 1 cut(s) 429
BstBI TTCGAA 1 cut(s) 962
BstC8I GCNNGC 1 cut(s) 696
BstDEI CTNAG 4 cut(s) 299, 507, 830, 914
BstDSI CCRYGG 1 cut(s) 80
BstENI CCTNNNNNAGG 1 cut(s) 144
BstFNI CGCG 1 cut(s) 694
BstHHI GCGC 1 cut(s) 694
BstKTI GATC 2 cut(s) 253, 592
BstMAI GTCTC 4 cut(s) 158, 510, 830, 934
BstMBI GATC 2 cut(s) 250, 589
BstMWI GCNNNNNNNGC 5 cut(s) 346, 429, 683, 698, 915
BstNI CCWGG 3 cut(s) 130, 609, 951
BstNSI RCATGY 1 cut(s) 629
BstSCI CCNGG 4 cut(s) 128, 540, 607, 949
BstSFI CTRYAG 4 cut(s) 217, 675, 699, 907
BstSLI GKGCMC 1 cut(s) 958
BstUI CGCG 1 cut(s) 694
BstV1I GCAGC 6 cut(s) 361, 685, 689, 713, 893, 921
BstX2I RGATCY 1 cut(s) 589
BstYI RGATCY 1 cut(s) 589
BsuRI GGCC 2 cut(s) 133, 150
BtgI CCRYGG 1 cut(s) 80
BtrI CACGTC 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 246, 672
BveI ACCTGC 1 cut(s) 114
Cac8I GCNNGC 1 cut(s) 696
CaiI CAGNNNCTG 2 cut(s) 129, 361
CfoI GCGC 1 cut(s) 694
Cfr13I GGNCC 2 cut(s) 126, 482
CsiI ACCWGGT 1 cut(s) 949
CviAII CATG 5 cut(s) 60, 70, 81, 164, 626
DdeI CTNAG 4 cut(s) 299, 507, 830, 914
DpnI GATC 2 cut(s) 252, 591
DpnII GATC 2 cut(s) 250, 589
Eco130I CCWWGG 2 cut(s) 80, 393
Eco31I GGTCTC 1 cut(s) 934
Eco32I GATATC 1 cut(s) 660
Eco47I GGWCC 2 cut(s) 126, 482
Eco57I CTGAAG 2 cut(s) 623, 669
EcoNI CCTNNNNNAGG 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 126
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 3 cut(s) 128, 607, 949
EcoRV GATATC 1 cut(s) 660
EcoT14I CCWWGG 2 cut(s) 80, 393
EcoT22I ATGCAT 1 cut(s) 760
ErhI CCWWGG 2 cut(s) 80, 393
Esp3I CGTCTC 1 cut(s) 158
FaeI CATG 5 cut(s) 63, 73, 84, 167, 629
FaqI GGGAC 4 cut(s) 537, 614, 654, 870
FatI CATG 5 cut(s) 59, 69, 80, 163, 625
FblI GTMKAC 1 cut(s) 401
Fnu4HI GCNGC 7 cut(s) 168, 350, 678, 699, 702, 907, 910
Fsp4HI GCNGC 7 cut(s) 168, 350, 678, 699, 702, 907, 910
FspBI CTAG 1 cut(s) 639
GlaI GCGC 1 cut(s) 693
GluI GCNGC 7 cut(s) 168, 350, 678, 699, 702, 907, 910
GsuI CTGGAG 1 cut(s) 951
HaeIII GGCC 2 cut(s) 133, 150
HapII CCGG 2 cut(s) 263, 542
HhaI GCGC 1 cut(s) 694
Hin1II CATG 5 cut(s) 63, 73, 84, 167, 629
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HinfI GANTC 5 cut(s) 160, 243, 667, 764, 794
HpaII CCGG 2 cut(s) 263, 542
HphI GGTGA 2 cut(s) 319, 803
Hpy166II GTNNAC 3 cut(s) 402, 482, 956
Hpy188I TCNGA 5 cut(s) 66, 146, 510, 649, 763
Hpy188III TCNNGA 8 cut(s) 157, 182, 263, 282, 639, 715, 728, 930
Hpy8I GTNNAC 3 cut(s) 402, 482, 956
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 7 cut(s) 144, 411, 512, 566, 788, 958, 969
HpyCH4III ACNGT 3 cut(s) 363, 472, 711
HpyCH4IV ACGT 1 cut(s) 114
HpyF10VI GCNNNNNNNGC 5 cut(s) 346, 429, 683, 698, 915
HpyF3I CTNAG 4 cut(s) 299, 507, 830, 914
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 5 cut(s) 63, 73, 84, 167, 629
HspAI GCGC 1 cut(s) 692
Kpn2I TCCGGA 1 cut(s) 262
Kzo9I GATC 2 cut(s) 250, 589
LmnI GCTCC 2 cut(s) 874, 932
Lsp1109I GCAGC 6 cut(s) 361, 685, 689, 713, 893, 921
LweI GCATC 2 cut(s) 114, 767
MabI ACCWGGT 1 cut(s) 949
MaeI CTAG 1 cut(s) 639
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 769
MalI GATC 2 cut(s) 252, 591
MboI GATC 2 cut(s) 250, 589
MboII GAAGA 5 cut(s) 18, 27, 30, 654, 730
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 958
MluCI AATT 7 cut(s) 46, 212, 225, 257, 438, 525, 854
MlyI GAGTC 1 cut(s) 676
Mph1103I ATGCAT 1 cut(s) 760
MroI TCCGGA 1 cut(s) 262
MseI TTAA 4 cut(s) 255, 546, 785, 891
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 263, 542
MspR9I CCNGG 4 cut(s) 130, 542, 609, 951
MvaI CCWGG 3 cut(s) 130, 609, 951
MvnI CGCG 1 cut(s) 694
MwoI GCNNNNNNNGC 5 cut(s) 346, 429, 683, 698, 915
NciI CCSGG 1 cut(s) 542
NcoI CCATGG 1 cut(s) 80
NdeII GATC 2 cut(s) 250, 589
NlaIII CATG 5 cut(s) 63, 73, 84, 167, 629
NlaIV GGNNCC 1 cut(s) 180
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 1 cut(s) 629
NspV TTCGAA 1 cut(s) 962
PfeI GAWTC 4 cut(s) 160, 243, 764, 794
PkrI GCNGC 7 cut(s) 169, 351, 679, 700, 703, 908, 911
PleI GAGTC 1 cut(s) 675
PpsI GAGTC 1 cut(s) 675
PpuMI RGGWCCY 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 126
Psp6I CCWGG 3 cut(s) 128, 607, 949
PspGI CCWGG 3 cut(s) 128, 607, 949
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 2 cut(s) 126, 482
PspPPI RGGWCCY 1 cut(s) 126
PstI CTGCAG 3 cut(s) 679, 703, 911
PstNI CAGNNNCTG 2 cut(s) 129, 361
PsuI RGATCY 1 cut(s) 589
SaqAI TTAA 4 cut(s) 255, 546, 785, 891
SatI GCNGC 7 cut(s) 168, 350, 678, 699, 702, 907, 910
Sau3AI GATC 2 cut(s) 250, 589
Sau96I GGNCC 2 cut(s) 126, 482
SchI GAGTC 1 cut(s) 676
ScrFI CCNGG 4 cut(s) 130, 542, 609, 951
SduI GDGCHC 1 cut(s) 958
SexAI ACCWGGT 1 cut(s) 949
SfaNI GCATC 2 cut(s) 114, 767
SfcI CTRYAG 4 cut(s) 217, 675, 699, 907
SfuI TTCGAA 1 cut(s) 962
SinI GGWCC 2 cut(s) 126, 482
Sse9I AATT 7 cut(s) 46, 212, 225, 257, 438, 525, 854
SsiI CCGC 1 cut(s) 168
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 4 cut(s) 128, 540, 607, 949
StyI CCWWGG 2 cut(s) 80, 393
TaaI ACNGT 3 cut(s) 363, 472, 711
TaiI ACGT 1 cut(s) 117
TaqI TCGA 5 cut(s) 117, 665, 716, 946, 962
TasI AATT 7 cut(s) 46, 212, 225, 257, 438, 525, 854
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 4 cut(s) 160, 243, 764, 794
Tru1I TTAA 4 cut(s) 255, 546, 785, 891
Tru9I TTAA 4 cut(s) 255, 546, 785, 891
TscAI CASTG 2 cut(s) 253, 679
TseI GCWGC 6 cut(s) 349, 677, 698, 701, 906, 909
TspDTI ATGAA 2 cut(s) 152, 531
TspRI CASTG 2 cut(s) 253, 679
VneI GTGCAC 1 cut(s) 954
VpaK11BI GGWCC 2 cut(s) 126, 482
XagI CCTNNNNNAGG 1 cut(s) 144
XapI RAATTY 4 cut(s) 212, 225, 257, 438
XbaI TCTAGA 1 cut(s) 638
XceI RCATGY 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 926
XmiI GTMKAC 1 cut(s) 401
XspI CTAG 1 cut(s) 639
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.