FvH4_6g09540

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001474
Physical Location & Seq
Forward (+)
5697982 .. 5700156
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09540.t1

Sequence Viewer

Length: 447 bp
ATGGCGTCCAAGCGCATCTTGAAGGAGCTTAAGGATTTGCAGAAGGATCCTCCCACTTCCTGCAGCGCCGGTCCTGTGGCTGAGGACATGTTTCATTGGCAAGCGACGATTATGGGACCCCCTGATAGCCCATATGCAGGAGGTGTATTCCTTGTGACCATTCATTTCCCTCCTGATTATCCTTTTAAACCGCCTAAGGTTGCATTTAGGACTAAGGTCTTCCACCCGAATATCAATAGCAATGGAAGCATTTGTCTTGATATCCTTAAAGAACAGTGGAGTCCAGCCCTTACTATCTCTAAGGTATTGCTCTCAATCTGCTCATTGCTGACTGACCCAAATCCTGATGACCCCCTTGTACCGGAGATCGCCCACATGTATAAAACTGATCGAGCCAAGTATGAGTCGACTGCACGTAGCTGGACTCAGAAGTATGCAATGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

149

Amino Acids

16.52

Weight (kDa)

7.72

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 4e-55 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 407
AciI CCGC 1 cut(s) 191
AclWI GGATC 2 cut(s) 41, 54
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 2 cut(s) 137, 361
AflII CTTAAG 1 cut(s) 29
AflIII ACRYGT 2 cut(s) 87, 375
AgsI TTSAA 1 cut(s) 22
AluBI AGCT 2 cut(s) 28, 420
AluI AGCT 2 cut(s) 28, 420
AlwI GGATC 2 cut(s) 41, 54
ApeKI GCWGC 1 cut(s) 63
AspLEI GCGC 2 cut(s) 15, 68
AspS9I GGNCC 2 cut(s) 71, 116
AvaII GGWCC 2 cut(s) 71, 116
AxyI CCTNAGG 1 cut(s) 195
BamHI GGATCC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 211
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 61
BfoI RGCGCY 1 cut(s) 69
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme18I GGWCC 2 cut(s) 71, 116
BmgT120I GGNCC 2 cut(s) 71, 116
BmiI GGNNCC 3 cut(s) 48, 117, 118
BmsI GCATC 1 cut(s) 24
BoxI GACNNNNGTC 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 211
Bpu10I CCTNAGC 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 361
Bse118I RCCGGY 1 cut(s) 68
Bse21I CCTNAGG 1 cut(s) 195
Bse3DI GCAATG 3 cut(s) 247, 323, 444
BseLI CCNNNNNNNGG 2 cut(s) 137, 361
BseMI GCAATG 3 cut(s) 247, 323, 444
BseMII CTCAG 2 cut(s) 72, 440
BseXI GCAGC 1 cut(s) 75
BsgI GTGCAG 1 cut(s) 396
BsiSI CCGG 2 cut(s) 69, 362
BslFI GGGAC 1 cut(s) 129
BslI CCNNNNNNNGG 2 cut(s) 137, 361
BsmFI GGGAC 1 cut(s) 129
Bsp143I GATC 3 cut(s) 46, 366, 388
BspACI CCGC 1 cut(s) 191
BspCNI CTCAG 2 cut(s) 73, 439
BspLI GGNNCC 3 cut(s) 48, 117, 118
BspMAI CTGCAG 1 cut(s) 65
BspPI GGATC 2 cut(s) 41, 54
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 3 cut(s) 247, 323, 444
BsrFI RCCGGY 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 68
BssMI GATC 3 cut(s) 46, 366, 388
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 5 cut(s) 81, 195, 213, 300, 426
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 15, 68
BstKTI GATC 3 cut(s) 49, 369, 391
BstMBI GATC 3 cut(s) 46, 366, 388
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstNSI RCATGY 2 cut(s) 91, 379
BstPAI GACNNNNGTC 1 cut(s) 215
BstSFI CTRYAG 1 cut(s) 61
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 102
CfoI GCGC 2 cut(s) 15, 68
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 71, 116
Csp6I GTAC 1 cut(s) 359
CviAII CATG 2 cut(s) 88, 376
CviJI RGCY 6 cut(s) 28, 80, 129, 287, 395, 420
CviKI_1 RGCY 6 cut(s) 28, 80, 129, 287, 395, 420
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 5 cut(s) 81, 195, 213, 300, 426
DpnI GATC 3 cut(s) 48, 368, 390
DpnII GATC 3 cut(s) 46, 366, 388
DraI TTTAAA 1 cut(s) 187
Eco32I GATATC 1 cut(s) 262
Eco47I GGWCC 2 cut(s) 71, 116
Eco81I CCTNAGG 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 116
EcoRV GATATC 1 cut(s) 262
FaeI CATG 2 cut(s) 91, 379
FaiI YATR 8 cut(s) 89, 113, 133, 135, 377, 381, 402, 435
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 129
FatI CATG 2 cut(s) 87, 375
FauNDI CATATG 1 cut(s) 133
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 1 cut(s) 64
Fsp4HI GCNGC 1 cut(s) 64
GlaI GCGC 2 cut(s) 14, 67
GluI GCNGC 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 69
HapII CCGG 2 cut(s) 69, 362
HhaI GCGC 2 cut(s) 15, 68
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 91, 379
Hin6I GCGC 2 cut(s) 13, 66
HinP1I GCGC 2 cut(s) 13, 66
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 3 cut(s) 280, 404, 424
HpaII CCGG 2 cut(s) 69, 362
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 1 cut(s) 429
Hpy188III TCNNGA 4 cut(s) 19, 173, 257, 344
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 2 cut(s) 16, 37
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 6 cut(s) 40, 63, 137, 203, 413, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 5 cut(s) 81, 195, 213, 300, 426
HpySE526I ACGT 1 cut(s) 415
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 91, 379
HspAI GCGC 2 cut(s) 13, 66
KflI GGGWCCC 1 cut(s) 116
Kzo9I GATC 3 cut(s) 46, 366, 388
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 1 cut(s) 75
LweI GCATC 1 cut(s) 24
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 48, 368, 390
MboI GATC 3 cut(s) 46, 366, 388
MboII GAAGA 1 cut(s) 211
MflI RGATCY 1 cut(s) 46
MlyI GAGTC 3 cut(s) 289, 413, 418
MnlI CCTC 4 cut(s) 60, 76, 134, 180
MseI TTAA 3 cut(s) 30, 186, 267
MspCI CTTAAG 1 cut(s) 29
MspI CCGG 2 cut(s) 69, 362
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeI CATATG 1 cut(s) 133
NdeII GATC 3 cut(s) 46, 366, 388
NlaIII CATG 2 cut(s) 91, 379
NlaIV GGNNCC 3 cut(s) 48, 117, 118
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 2 cut(s) 91, 379
PciI ACATGT 2 cut(s) 87, 375
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 3 cut(s) 288, 412, 418
PpsI GAGTC 3 cut(s) 288, 412, 418
Ppu21I YACGTR 1 cut(s) 416
PpuMI RGGWCCY 1 cut(s) 116
PscI ACATGT 2 cut(s) 87, 375
PshAI GACNNNNGTC 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 48, 117, 118
PspPI GGNCC 2 cut(s) 71, 116
PspPPI RGGWCCY 1 cut(s) 116
PstI CTGCAG 1 cut(s) 65
PsuI RGATCY 1 cut(s) 46
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
SalI GTCGAC 1 cut(s) 406
SaqAI TTAA 3 cut(s) 30, 186, 267
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 3 cut(s) 46, 366, 388
Sau96I GGNCC 2 cut(s) 71, 116
SchI GAGTC 3 cut(s) 289, 413, 418
SetI ASST 7 cut(s) 30, 145, 201, 219, 306, 418, 422
SfaNI GCATC 1 cut(s) 24
SfcI CTRYAG 1 cut(s) 61
SinI GGWCC 2 cut(s) 71, 116
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SsiI CCGC 1 cut(s) 191
TaaI ACNGT 1 cut(s) 276
TaiI ACGT 1 cut(s) 418
TaqI TCGA 2 cut(s) 391, 407
Tru1I TTAA 3 cut(s) 30, 186, 267
Tru9I TTAA 3 cut(s) 30, 186, 267
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 2 cut(s) 83, 152
TspRI CASTG 1 cut(s) 281
Vha464I CTTAAG 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 71, 116
XceI RCATGY 2 cut(s) 91, 379
XmiI GTMKAC 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.