FvH4_7g15850

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001474
Physical Location & Seq
Forward (+)
13689264 .. 13692189
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g15850.t1

Sequence Viewer

Length: 447 bp
ATGGCGTCCAAGCGGATCTTGAAGGAGCTCAAGGATCTCCAGAAGGATCCCCCGACCTCATGCAGCGCCGGGCCTGTTGCTGAAGACATGTTTCATTGGCAAGCGACGATAATGGGCCCTCCTGATAGTCCTTATGCAGGGGGTGTCTTTTTGGTTACCATTCATTTCCCTCCTGATTATCCGTTTAAACCACCTAAGGTTGCATTTAGGACTAAGGTCTTTCATCCAAATATCAATAGCAACGGAAGTATCTGCCTTGACATTTTGAAAGAGCAGTGGAGCCCTGCTCTGACCATATCCAAGGTGTTGCTATCCATTTGTTCCCTGTTGACTGACCCCAACCCGGATGATCCCCTGGTGCCGGAAATTGCTCACATGTACAAGACAGACCGGTCAAAGTACGAGACAACCGCTAGGAGCTGGACCCAGAAGTATGCTATGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

149

Amino Acids

16.55

Weight (kDa)

7.72

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 3.7e-55 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 358
AciI CCGC 2 cut(s) 13, 411
AclWI GGATC 5 cut(s) 23, 41, 42, 54, 344
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 380, 401
AfiI CCNNNNNNNGG 3 cut(s) 137, 343, 361
AflIII ACRYGT 2 cut(s) 87, 375
AgeI ACCGGT 1 cut(s) 390
AgsI TTSAA 2 cut(s) 22, 268
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 2 cut(s) 28, 420
AluI AGCT 2 cut(s) 28, 420
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 5 cut(s) 23, 41, 42, 54, 344
AoxI GGCC 2 cut(s) 71, 115
ApaI GGGCCC 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 63
AsiGI ACCGGT 1 cut(s) 390
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 4 cut(s) 71, 115, 116, 423
AsuC2I CCSGG 2 cut(s) 70, 344
AvaII GGWCC 1 cut(s) 423
AxyI CCTNAGG 1 cut(s) 195
BaeGI GKGCMC 1 cut(s) 119
BaeI ACNNNNGTAYC 2 cut(s) 232, 265
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 3 cut(s) 30, 119, 284
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 75
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 2 cut(s) 70, 344
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 69
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 3 cut(s) 70, 344, 356
Bme18I GGWCC 1 cut(s) 423
BmgT120I GGNCC 4 cut(s) 71, 115, 116, 423
BmiI GGNNCC 5 cut(s) 48, 117, 281, 360, 425
BmrFI CCNGG 3 cut(s) 70, 344, 356
BoxI GACNNNNGTC 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 271, 303
BpmI CTGGAG 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 2 cut(s) 70, 344
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 300, 354
BsaWI WCCGGW 1 cut(s) 390
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 343, 361
Bse118I RCCGGY 1 cut(s) 390
Bse21I CCTNAGG 1 cut(s) 195
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 300, 354
BseGI GGATG 2 cut(s) 223, 352
BseLI CCNNNNNNNGG 3 cut(s) 137, 343, 361
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 1 cut(s) 75
BshFI GGCC 2 cut(s) 73, 117
BshNI GGYRCC 1 cut(s) 358
BshTI ACCGGT 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 4 cut(s) 69, 344, 362, 391
BslI CCNNNNNNNGG 3 cut(s) 137, 343, 361
BsmAI GTCTC 1 cut(s) 398
BsnI GGCC 2 cut(s) 73, 117
Bsp120I GGGCCC 1 cut(s) 115
Bsp1286I GDGCHC 3 cut(s) 30, 119, 284
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 15, 34, 46, 349
BspACI CCGC 2 cut(s) 13, 411
BspANI GGCC 2 cut(s) 73, 117
BspLI GGNNCC 5 cut(s) 48, 117, 281, 360, 425
BspPI GGATC 5 cut(s) 23, 41, 42, 54, 344
BspT107I GGYRCC 1 cut(s) 358
BsrFI RCCGGY 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 378
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 2 cut(s) 300, 354
BssMI GATC 4 cut(s) 15, 34, 46, 349
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 356
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 2 cut(s) 195, 213
BstEII GGTNACC 1 cut(s) 154
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 223, 352
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 4 cut(s) 18, 37, 49, 352
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 4 cut(s) 15, 34, 46, 349
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 2 cut(s) 91, 379
BstPAI GACNNNNGTC 1 cut(s) 215
BstPI GGTNACC 1 cut(s) 154
BstSCI CCNGG 3 cut(s) 68, 342, 354
BstSLI GKGCMC 1 cut(s) 119
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 3 cut(s) 15, 34, 46
BstYI RGATCY 3 cut(s) 15, 34, 46
Bsu36I CCTNAGG 1 cut(s) 195
BsuRI GGCC 2 cut(s) 73, 117
BtsCI GGATG 2 cut(s) 223, 352
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 102
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 1 cut(s) 390
Cfr13I GGNCC 4 cut(s) 71, 115, 116, 423
Csp6I GTAC 2 cut(s) 379, 400
CspAI ACCGGT 1 cut(s) 390
CviAII CATG 3 cut(s) 60, 88, 376
CviJI RGCY 5 cut(s) 28, 73, 117, 282, 420
CviKI_1 RGCY 5 cut(s) 28, 73, 117, 282, 420
CviQI GTAC 2 cut(s) 379, 400
DdeI CTNAG 2 cut(s) 195, 213
DpnI GATC 4 cut(s) 17, 36, 48, 351
DpnII GATC 4 cut(s) 15, 34, 46, 349
DraI TTTAAA 1 cut(s) 187
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 3 cut(s) 30, 119, 284
Eco47I GGWCC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 102
Eco81I CCTNAGG 1 cut(s) 195
Eco91I GGTNACC 1 cut(s) 154
EcoICRI GAGCTC 1 cut(s) 28
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoO109I RGGNCCY 1 cut(s) 116
EcoO65I GGTNACC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 3 cut(s) 30, 119, 284
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 3 cut(s) 63, 91, 379
FaiI YATR 7 cut(s) 61, 89, 135, 296, 377, 435, 440
FalI AAGNNNNNCTT 1 cut(s) 34
FatI CATG 3 cut(s) 59, 87, 375
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 2 cut(s) 210, 359
FriOI GRGCYC 3 cut(s) 30, 119, 284
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 1 cut(s) 414
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 23
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 2 cut(s) 73, 117
HapII CCGG 4 cut(s) 69, 344, 362, 391
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 63, 91, 379
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HpaII CCGG 4 cut(s) 69, 344, 362, 391
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 4 cut(s) 19, 40, 122, 173
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 2 cut(s) 16, 37
HpyCH4V TGCA 3 cut(s) 63, 137, 203
HpyF3I CTNAG 2 cut(s) 195, 213
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 63, 91, 379
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 4 cut(s) 15, 34, 46, 349
LmnI GCTCC 3 cut(s) 25, 279, 417
Lsp1109I GCAGC 1 cut(s) 75
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 154
MalI GATC 4 cut(s) 17, 36, 48, 351
MboI GATC 4 cut(s) 15, 34, 46, 349
MboII GAAGA 1 cut(s) 95
MflI RGATCY 3 cut(s) 15, 34, 46
MhlI GDGCHC 3 cut(s) 30, 119, 284
MluCI AATT 1 cut(s) 366
MnlI CCTC 3 cut(s) 67, 129, 180
MseI TTAA 1 cut(s) 186
MspI CCGG 4 cut(s) 69, 344, 362, 391
MspR9I CCNGG 3 cut(s) 70, 344, 356
MssI GTTTAAAC 1 cut(s) 187
MvaI CCWGG 1 cut(s) 356
NciI CCSGG 2 cut(s) 70, 344
NdeII GATC 4 cut(s) 15, 34, 46, 349
NlaIII CATG 3 cut(s) 63, 91, 379
NlaIV GGNNCC 5 cut(s) 48, 117, 281, 360, 425
NspI RCATGY 2 cut(s) 91, 379
PciI ACATGT 2 cut(s) 87, 375
PinAI ACCGGT 1 cut(s) 390
PkrI GCNGC 1 cut(s) 65
PmeI GTTTAAAC 1 cut(s) 187
PscI ACATGT 2 cut(s) 87, 375
PshAI GACNNNNGTC 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 354
PspEI GGTNACC 1 cut(s) 154
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 5 cut(s) 48, 117, 281, 360, 425
PspOMI GGGCCC 1 cut(s) 115
PspPI GGNCC 4 cut(s) 71, 115, 116, 423
PsuI RGATCY 3 cut(s) 15, 34, 46
RsaI GTAC 2 cut(s) 380, 401
RsaNI GTAC 2 cut(s) 379, 400
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 4 cut(s) 15, 34, 46, 349
Sau96I GGNCC 4 cut(s) 71, 115, 116, 423
ScrFI CCNGG 3 cut(s) 70, 344, 356
SduI GDGCHC 3 cut(s) 30, 119, 284
SetI ASST 7 cut(s) 30, 59, 196, 201, 219, 306, 422
SinI GGWCC 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 1 cut(s) 366
SsiI CCGC 2 cut(s) 13, 411
SspMI CTAG 1 cut(s) 414
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 68, 342, 354
StyI CCWWGG 1 cut(s) 300
TasI AATT 1 cut(s) 366
TatI WGTACW 1 cut(s) 378
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 1 cut(s) 281
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 3 cut(s) 83, 152, 212
TspGWI ACGGA 2 cut(s) 171, 258
TspRI CASTG 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 423
XagI CCTNNNNNAGG 1 cut(s) 135
XceI RCATGY 2 cut(s) 91, 379
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.