Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001474

Physical Location & Seq

Genomic Coordinates Reverse (-)

26730983 .. 26733053

Gene Structure 2071 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1176600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 447 bp

ATGGCGTCGAAGCGCATCTTGAAAGAGCTCAAGGATTTGCAGAAGGATCCTCCAACTTCTTGCAGCGCAGGTCCTGTGGCTGAGGACATGTTTCATTGGCAAGCAACGATTATGGGACCCCCTGATAGCCCATACGCAGGAGGTGTATTCCTGGTCACCATTCATTTCCCTCCAGATTATCCTTTCAAGCCCCCTAAGGTTGCATTTAGGACTAAGGTCTTCCACCCGAATATCAATAGCAACGGAAGCATTTGTCTTGACATCCTAAAAGAGCAATGGAGCCCAGCACTTACTATCTCTAAGGTATTGCTCTCAATCTGCTCATTGCTGACTGACCCAAATCCTGATGACCCTCTCGTGCCGGAGATCGCCCACATGTACAAAACTGATCGAGCCAAGTACGAATCCACTGCACGTAGCTGGACTCAGAAATATGCAATGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624

Pfam Domains

Protein Families

UQ_con

Protein Analysis

149

Amino Acids

16.52

Weight (kDa)

7.72

Isoelectric Point (pI)

47.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UQ_con	PF00179	5 - 141	4e-55	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001474)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08690 AT3G08690 AT3G08700 AT3G08700
fragaria_vesca	FvH4_3g11640 FvH4_6g09540 FvH4_7g15850
malus_domestica	MD01G1075300.v1.1 MD01G1214300.v1.1 MD04G1176600.v1.1 MD07G1144200.v1.1 MD12G1190900.v1.1
prunus_persica	Prupe.2G183100_v2.0.a1 Prupe.2G306300_v2.0.a1 Prupe.2G306300_v2.0.a1 Prupe.2G306300_v2.0.a1 Prupe.6G296100_v2.0.a1 Prupe.6G296100_v2.0.a1 Prupe.6G296100_v2.0.a1
pyrus_communis	pycom04g15580 pycom07g14260 pycom12g17820 pycom15g33780
rosa_chinensis	RchiOBHm_Chr1g0358671 RchiOBHm_Chr1g0380011 RchiOBHm_Chr3g0460141 RchiOBHm_Chr5g0018691
rosa_laevigata	RLG00000032387
rosa_multiflora	Rmu_co8206768.1_g000001 Rmu_sc0000449.1_g000050 Rmu_sc0001609.1_g000029 Rmu_sc0010911.1_g000002 Rmu_sc0025354.1_g000001
rosa_roxburghii	Rroxscaffold_1G00059080 Rroxscaffold_4G00279800 Rroxscaffold_4G00297720 Rroxscaffold_6G00419800
rosa_rugosa	Rorug01G0416400 Rorug01G0416500 Rorug03G0040400 Rorug04G0115100 Rorug05G0046000
rosa_samantha	Rh1AG441000 Rh1BG396700 Rh3DG102700 Rh5AG137000 Rh5BG135800 Rh5CG147700
rosa_wichuraiana	Rw1G038350 Rw3G008300 Rw5G012140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	59
AclWI	GGATC	2 cut(s)	41, 54
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	2 cut(s)	380, 401
AfiI	CCNNNNNNNGG	1 cut(s)	137
AflIII	ACRYGT	2 cut(s)	87, 375
AgsI	TTSAA	2 cut(s)	22, 187
AjnI	CCWGG	1 cut(s)	150
AluBI	AGCT	2 cut(s)	28, 420
AluI	AGCT	2 cut(s)	28, 420
Alw21I	GWGCWC	1 cut(s)	30
AlwI	GGATC	2 cut(s)	41, 54
AlwNI	CAGNNNCTG	1 cut(s)	74
ApeKI	GCWGC	1 cut(s)	63
AspLEI	GCGC	2 cut(s)	15, 68
AspS9I	GGNCC	2 cut(s)	71, 116
AsuHPI	GGTGA	1 cut(s)	148
AvaII	GGWCC	2 cut(s)	71, 116
AxyI	CCTNAGG	1 cut(s)	195
BamHI	GGATCC	1 cut(s)	46
BanII	GRGCYC	2 cut(s)	30, 284
BauI	CACGAG	1 cut(s)	356
BbsI	GAAGAC	1 cut(s)	211
Bbv12I	GWGCWC	1 cut(s)	30
BbvCI	CCTCAGC	1 cut(s)	81
BbvI	GCAGC	1 cut(s)	75
BcgI	CGANNNNNNTGC	2 cut(s)	392, 426
BciT130I	CCWGG	1 cut(s)	152
BfuAI	ACCTGC	1 cut(s)	59
BisI	GCNGC	1 cut(s)	64
BlsI	GCNGC	1 cut(s)	65
Bme1390I	CCNGG	1 cut(s)	152
Bme18I	GGWCC	2 cut(s)	71, 116
BmgT120I	GGNCC	2 cut(s)	71, 116
BmiI	GGNNCC	4 cut(s)	48, 117, 118, 281
BmrFI	CCNGG	1 cut(s)	152
BmsI	GCATC	1 cut(s)	24
BoxI	GACNNNNGTC	1 cut(s)	215
BpiI	GAAGAC	1 cut(s)	211
BpmI	CTGGAG	1 cut(s)	156
Bpu10I	CCTNAGC	1 cut(s)	81
BpuEI	CTTGAG	1 cut(s)	14
BsaAI	YACGTR	1 cut(s)	416
BsaHI	GRCGYC	1 cut(s)	5
Bsc4I	CCNNNNNNNGG	1 cut(s)	137
Bse21I	CCTNAGG	1 cut(s)	195
Bse3DI	GCAATG	3 cut(s)	281, 323, 444
BseBI	CCWGG	1 cut(s)	152
BseGI	GGATG	1 cut(s)	261
BseLI	CCNNNNNNNGG	1 cut(s)	137
BseMI	GCAATG	3 cut(s)	281, 323, 444
BseMII	CTCAG	2 cut(s)	72, 440
BseXI	GCAGC	1 cut(s)	75
BseYI	CCCAGC	1 cut(s)	283
BsgI	GTGCAG	1 cut(s)	396
BsiHKAI	GWGCWC	1 cut(s)	30
BsiSI	CCGG	1 cut(s)	362
BslFI	GGGAC	1 cut(s)	129
BslI	CCNNNNNNNGG	1 cut(s)	137
BsmFI	GGGAC	1 cut(s)	129
Bsp1286I	GDGCHC	2 cut(s)	30, 284
Bsp1407I	TGTACA	1 cut(s)	378
Bsp143I	GATC	3 cut(s)	46, 366, 388
BspCNI	CTCAG	2 cut(s)	73, 439
BspLI	GGNNCC	4 cut(s)	48, 117, 118, 281
BspMI	ACCTGC	1 cut(s)	59
BspPI	GGATC	2 cut(s)	41, 54
BsrDI	GCAATG	3 cut(s)	281, 323, 444
BsrGI	TGTACA	1 cut(s)	378
BssMI	GATC	3 cut(s)	46, 366, 388
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	356
Bst2BI	CACGAG	1 cut(s)	356
Bst2UI	CCWGG	1 cut(s)	152
BstACI	GRCGYC	1 cut(s)	5
BstAUI	TGTACA	1 cut(s)	378
BstBAI	YACGTR	1 cut(s)	416
BstC8I	GCNNGC	1 cut(s)	102
BstDEI	CTNAG	5 cut(s)	81, 195, 213, 300, 426
BstEII	GGTNACC	1 cut(s)	154
BstF5I	GGATG	1 cut(s)	261
BstHHI	GCGC	2 cut(s)	15, 68
BstKTI	GATC	3 cut(s)	49, 369, 391
BstMBI	GATC	3 cut(s)	46, 366, 388
BstMWI	GCNNNNNNNGC	1 cut(s)	246
BstNI	CCWGG	1 cut(s)	152
BstNSI	RCATGY	2 cut(s)	91, 379
BstPAI	GACNNNNGTC	1 cut(s)	215
BstPI	GGTNACC	1 cut(s)	154
BstSCI	CCNGG	1 cut(s)	150
BstV1I	GCAGC	1 cut(s)	75
BstV2I	GAAGAC	1 cut(s)	211
BstX2I	RGATCY	1 cut(s)	46
BstYI	RGATCY	1 cut(s)	46
Bsu36I	CCTNAGG	1 cut(s)	195
BtsCI	GGATG	1 cut(s)	261
BtsI	GCAGTG	1 cut(s)	408
BtsIMutI	CAGTG	1 cut(s)	408
BveI	ACCTGC	1 cut(s)	59
Cac8I	GCNNGC	1 cut(s)	102
CaiI	CAGNNNCTG	1 cut(s)	74
CfoI	GCGC	2 cut(s)	15, 68
Cfr13I	GGNCC	2 cut(s)	71, 116
Csp6I	GTAC	2 cut(s)	379, 400
CviAII	CATG	2 cut(s)	88, 376
CviJI	RGCY	7 cut(s)	28, 80, 129, 190, 282, 395, 420
CviKI_1	RGCY	7 cut(s)	28, 80, 129, 190, 282, 395, 420
CviQI	GTAC	2 cut(s)	379, 400
DdeI	CTNAG	5 cut(s)	81, 195, 213, 300, 426
DpnI	GATC	3 cut(s)	48, 368, 390
DpnII	GATC	3 cut(s)	46, 366, 388
Ecl136II	GAGCTC	1 cut(s)	28
Eco24I	GRGCYC	2 cut(s)	30, 284
Eco47I	GGWCC	2 cut(s)	71, 116
Eco53kI	GAGCTC	1 cut(s)	28
Eco81I	CCTNAGG	1 cut(s)	195
Eco91I	GGTNACC	1 cut(s)	154
EcoICRI	GAGCTC	1 cut(s)	28
EcoO109I	RGGNCCY	2 cut(s)	71, 116
EcoO65I	GGTNACC	1 cut(s)	154
EcoRII	CCWGG	1 cut(s)	150
EcoT38I	GRGCYC	2 cut(s)	30, 284
FaeI	CATG	2 cut(s)	91, 379
FaiI	YATR	5 cut(s)	89, 113, 133, 377, 435
FalI	AAGNNNNNCTT	1 cut(s)	34
FaqI	GGGAC	1 cut(s)	129
FatI	CATG	2 cut(s)	87, 375
Fnu4HI	GCNGC	1 cut(s)	64
FokI	GGATG	1 cut(s)	248
FriOI	GRGCYC	2 cut(s)	30, 284
Fsp4HI	GCNGC	1 cut(s)	64
GlaI	GCGC	2 cut(s)	14, 67
GluI	GCNGC	1 cut(s)	64
GsaI	CCCAGC	1 cut(s)	287
GsuI	CTGGAG	1 cut(s)	156
HapII	CCGG	1 cut(s)	362
HhaI	GCGC	2 cut(s)	15, 68
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	2 cut(s)	91, 379
Hin6I	GCGC	2 cut(s)	13, 66
HinP1I	GCGC	2 cut(s)	13, 66
HinfI	GANTC	2 cut(s)	404, 424
HpaII	CCGG	1 cut(s)	362
HphI	GGTGA	1 cut(s)	148
Hpy188I	TCNGA	1 cut(s)	429
Hpy188III	TCNNGA	4 cut(s)	19, 173, 257, 344
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	1 cut(s)	37
HpyCH4IV	ACGT	1 cut(s)	415
HpyCH4V	TGCA	5 cut(s)	40, 63, 203, 413, 437
HpyF10VI	GCNNNNNNNGC	1 cut(s)	246
HpyF3I	CTNAG	5 cut(s)	81, 195, 213, 300, 426
HpySE526I	ACGT	1 cut(s)	415
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	2 cut(s)	91, 379
HspAI	GCGC	2 cut(s)	13, 66
KflI	GGGWCCC	1 cut(s)	116
Kzo9I	GATC	3 cut(s)	46, 366, 388
LmnI	GCTCC	1 cut(s)	279
Lsp1109I	GCAGC	1 cut(s)	75
LweI	GCATC	1 cut(s)	24
MaeII	ACGT	1 cut(s)	415
MaeIII	GTNAC	1 cut(s)	154
MalI	GATC	3 cut(s)	48, 368, 390
MboI	GATC	3 cut(s)	46, 366, 388
MboII	GAAGA	1 cut(s)	211
MflI	RGATCY	1 cut(s)	46
MhlI	GDGCHC	2 cut(s)	30, 284
MlyI	GAGTC	1 cut(s)	418
MmeI	TCCRAC	1 cut(s)	77
MnlI	CCTC	5 cut(s)	60, 76, 134, 180, 363
MspI	CCGG	1 cut(s)	362
MspR9I	CCNGG	1 cut(s)	152
MvaI	CCWGG	1 cut(s)	152
MwoI	GCNNNNNNNGC	1 cut(s)	246
NdeII	GATC	3 cut(s)	46, 366, 388
NlaIII	CATG	2 cut(s)	91, 379
NlaIV	GGNNCC	4 cut(s)	48, 117, 118, 281
NmuCI	GTSAC	1 cut(s)	154
NspI	RCATGY	2 cut(s)	91, 379
PciI	ACATGT	2 cut(s)	87, 375
PfeI	GAWTC	1 cut(s)	404
PkrI	GCNGC	1 cut(s)	65
PleI	GAGTC	1 cut(s)	418
PpsI	GAGTC	1 cut(s)	418
Ppu21I	YACGTR	1 cut(s)	416
PpuMI	RGGWCCY	2 cut(s)	71, 116
PscI	ACATGT	2 cut(s)	87, 375
PshAI	GACNNNNGTC	1 cut(s)	215
Psp124BI	GAGCTC	1 cut(s)	30
Psp5II	RGGWCCY	2 cut(s)	71, 116
Psp6I	CCWGG	1 cut(s)	150
PspEI	GGTNACC	1 cut(s)	154
PspFI	CCCAGC	1 cut(s)	283
PspGI	CCWGG	1 cut(s)	150
PspN4I	GGNNCC	4 cut(s)	48, 117, 118, 281
PspPI	GGNCC	2 cut(s)	71, 116
PspPPI	RGGWCCY	2 cut(s)	71, 116
PstNI	CAGNNNCTG	1 cut(s)	74
PsuI	RGATCY	1 cut(s)	46
RsaI	GTAC	2 cut(s)	380, 401
RsaNI	GTAC	2 cut(s)	379, 400
SacI	GAGCTC	1 cut(s)	30
SatI	GCNGC	1 cut(s)	64
Sau3AI	GATC	3 cut(s)	46, 366, 388
Sau96I	GGNCC	2 cut(s)	71, 116
SchI	GAGTC	1 cut(s)	418
ScrFI	CCNGG	1 cut(s)	152
SduI	GDGCHC	2 cut(s)	30, 284
SetI	ASST	8 cut(s)	30, 73, 145, 201, 219, 306, 418, 422
SfaNI	GCATC	1 cut(s)	24
SinI	GGWCC	2 cut(s)	71, 116
SmlI	CTYRAG	1 cut(s)	29
SmoI	CTYRAG	1 cut(s)	29
SstI	GAGCTC	1 cut(s)	30
StyD4I	CCNGG	1 cut(s)	150
TaiI	ACGT	1 cut(s)	418
TaqI	TCGA	2 cut(s)	8, 391
TatI	WGTACW	1 cut(s)	378
TfiI	GAWTC	1 cut(s)	404
TscAI	CASTG	1 cut(s)	415
TseFI	GTSAC	1 cut(s)	154
TseI	GCWGC	1 cut(s)	63
Tsp45I	GTSAC	1 cut(s)	154
TspDTI	ATGAA	2 cut(s)	83, 152
TspGWI	ACGGA	1 cut(s)	258
TspRI	CASTG	1 cut(s)	415
VpaK11BI	GGWCC	2 cut(s)	71, 116
XceI	RCATGY	2 cut(s)	91, 379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.