Rroxscaffold_4G00279800

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001474
Physical Location & Seq
Reverse (-)
2483484 .. 2486213
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279800.1

Sequence Viewer

Length: 549 bp
ATGGCGTCGAAGCGGATCCTGAAGGAGCTTAAGGATTTGCAGAAGGATCCTCCTACGTCATGCAGCGCAGAATTGTGGTGTGCCGACATGTCATTACTGGTGTGGATCATGTATATTGATGTGTCAGCCATGCTCGGTTGTGAGAGCATGGCTATATCACTAGGGAATGTAGGTCCTGTTGCCGAAGACATGTTTCATTGGCAAGCAACAATTATGGGTCCCCCAGATAGTCCTTATGCTGGTGGTGTTTTTCTAGTTACTATTCATTTTCCGCCAGATTATCCATTTAAGCCACCAAAGGTGGCATTTAGAACAAAGGTATTCCACCCAAACATTAACAGCAATGGGAGCATTTGCCTCGACATCTTGAAGGAGCAATGGAGTCCTGCTCTAACCATTTCCAAGGTGTTGCTTTCAATCTGTTCACTGTTGACGGATCCAAATCCCGATGATCCATTGGTGCCAGAGATTGCTCACATGTACAAGACAGACAGGAACAAGTATGAGACAACAGCAAGAAGCTGGACCCAAAAATATGCCATGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

182

Amino Acids

20.32

Weight (kDa)

5.76

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 57 - 175 1.8e-49 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 460
AciI CCGC 2 cut(s) 13, 272
AclWI GGATC 8 cut(s) 10, 23, 41, 54, 113, 431, 444, 446
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 1 cut(s) 239
AflII CTTAAG 1 cut(s) 29
AflIII ACRYGT 3 cut(s) 87, 189, 477
AgsI TTSAA 2 cut(s) 370, 417
AluBI AGCT 2 cut(s) 28, 522
AluI AGCT 2 cut(s) 28, 522
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 8 cut(s) 10, 23, 41, 54, 113, 431, 444, 446
ApeKI GCWGC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 3 cut(s) 173, 218, 525
AvaII GGWCC 3 cut(s) 173, 218, 525
BamHI GGATCC 3 cut(s) 15, 46, 436
BanI GGYRCC 1 cut(s) 460
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 75
BcgI CGANNNNNNTGC 2 cut(s) 340, 374
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 2 cut(s) 161, 254
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme18I GGWCC 3 cut(s) 173, 218, 525
BmgT120I GGNCC 3 cut(s) 173, 218, 525
BmiI GGNNCC 7 cut(s) 17, 48, 219, 220, 438, 462, 527
BpiI GAAGAC 1 cut(s) 192
BplI GAGNNNNNCTC 2 cut(s) 373, 405
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 402, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 102
Bse3DI GCAATG 2 cut(s) 349, 383
Bse8I GATNNNNATC 1 cut(s) 441
BseDI CCNNGG 2 cut(s) 402, 540
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 2 cut(s) 349, 383
BseNI ACTGG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 75
BshNI GGYRCC 1 cut(s) 460
BslFI GGGAC 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 5 cut(s) 15, 46, 105, 436, 451
Bsp19I CCATGG 1 cut(s) 540
BspACI CCGC 2 cut(s) 13, 272
BspLI GGNNCC 7 cut(s) 17, 48, 219, 220, 438, 462, 527
BspPI GGATC 8 cut(s) 10, 23, 41, 54, 113, 431, 444, 446
BspT107I GGYRCC 1 cut(s) 460
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 2 cut(s) 349, 383
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 402, 540
BssMI GATC 5 cut(s) 15, 46, 105, 436, 451
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 402, 540
Bst4CI ACNGT 1 cut(s) 429
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 29
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 1 cut(s) 204
BstDSI CCRYGG 1 cut(s) 540
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 5 cut(s) 18, 49, 108, 439, 454
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 5 cut(s) 15, 46, 105, 436, 451
BstMWI GCNNNNNNNGC 1 cut(s) 348
BstNSI RCATGY 3 cut(s) 91, 193, 481
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 3 cut(s) 15, 46, 436
BstYI RGATCY 3 cut(s) 15, 46, 436
BtgI CCRYGG 1 cut(s) 540
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 3 cut(s) 173, 218, 525
Csp6I GTAC 1 cut(s) 481
CviAII CATG 8 cut(s) 60, 88, 109, 130, 148, 190, 478, 541
CviJI RGCY 6 cut(s) 28, 128, 152, 292, 522, 546
CviKI_1 RGCY 6 cut(s) 28, 128, 152, 292, 522, 546
CviQI GTAC 1 cut(s) 481
DpnI GATC 5 cut(s) 17, 48, 107, 438, 453
DpnII GATC 5 cut(s) 15, 46, 105, 436, 451
EciI GGCGGA 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 402, 540
Eco47I GGWCC 3 cut(s) 173, 218, 525
Eco57I CTGAAG 1 cut(s) 41
EcoO109I RGGNCCY 2 cut(s) 173, 218
EcoT14I CCWWGG 2 cut(s) 402, 540
ErhI CCWWGG 2 cut(s) 402, 540
FaeI CATG 8 cut(s) 63, 91, 112, 133, 151, 193, 481, 544
FaqI GGGAC 1 cut(s) 204
FatI CATG 8 cut(s) 59, 87, 108, 129, 147, 189, 477, 540
Fnu4HI GCNGC 1 cut(s) 64
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 2 cut(s) 161, 254
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 64
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 63, 91, 112, 133, 151, 193, 481, 544
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HinfI GANTC 1 cut(s) 382
Hpy166II GTNNAC 2 cut(s) 425, 432
Hpy188III TCNNGA 3 cut(s) 19, 367, 446
Hpy8I GTNNAC 2 cut(s) 425, 432
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 16, 37, 364
HpyCH4III ACNGT 1 cut(s) 429
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 2 cut(s) 40, 63
HpyF10VI GCNNNNNNNGC 1 cut(s) 348
HpySE526I ACGT 1 cut(s) 56
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 63, 91, 112, 133, 151, 193, 481, 544
HspAI GCGC 1 cut(s) 66
KflI GGGWCCC 1 cut(s) 218
Kzo9I GATC 5 cut(s) 15, 46, 105, 436, 451
LmnI GCTCC 3 cut(s) 25, 348, 373
Lsp1109I GCAGC 1 cut(s) 75
MaeI CTAG 2 cut(s) 161, 254
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 1 cut(s) 256
MalI GATC 5 cut(s) 17, 48, 107, 438, 453
MboI GATC 5 cut(s) 15, 46, 105, 436, 451
MboII GAAGA 1 cut(s) 197
MflI RGATCY 3 cut(s) 15, 46, 436
MluCI AATT 2 cut(s) 71, 210
MlyI GAGTC 1 cut(s) 391
MnlI CCTC 2 cut(s) 60, 368
MseI TTAA 3 cut(s) 30, 288, 336
MspCI CTTAAG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 348
NcoI CCATGG 1 cut(s) 540
NdeII GATC 5 cut(s) 15, 46, 105, 436, 451
NlaIII CATG 8 cut(s) 63, 91, 112, 133, 151, 193, 481, 544
NlaIV GGNNCC 7 cut(s) 17, 48, 219, 220, 438, 462, 527
NspI RCATGY 3 cut(s) 91, 193, 481
PciI ACATGT 3 cut(s) 87, 189, 477
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
PpuMI RGGWCCY 2 cut(s) 173, 218
PscI ACATGT 3 cut(s) 87, 189, 477
Psp5II RGGWCCY 2 cut(s) 173, 218
PspN4I GGNNCC 7 cut(s) 17, 48, 219, 220, 438, 462, 527
PspPI GGNCC 3 cut(s) 173, 218, 525
PspPPI RGGWCCY 2 cut(s) 173, 218
PsuI RGATCY 3 cut(s) 15, 46, 436
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SaqAI TTAA 3 cut(s) 30, 288, 336
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 5 cut(s) 15, 46, 105, 436, 451
Sau96I GGNCC 3 cut(s) 173, 218, 525
SchI GAGTC 1 cut(s) 391
SetI ASST 7 cut(s) 30, 59, 175, 303, 321, 408, 524
SinI GGWCC 3 cut(s) 173, 218, 525
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 2 cut(s) 71, 210
SsiI CCGC 2 cut(s) 13, 272
SspMI CTAG 2 cut(s) 161, 254
StyI CCWWGG 2 cut(s) 402, 540
TaaI ACNGT 1 cut(s) 429
TaiI ACGT 1 cut(s) 59
TaqI TCGA 2 cut(s) 8, 360
TasI AATT 2 cut(s) 71, 210
TatI WGTACW 1 cut(s) 480
Tru1I TTAA 3 cut(s) 30, 288, 336
Tru9I TTAA 3 cut(s) 30, 288, 336
TscAI CASTG 1 cut(s) 432
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 2 cut(s) 185, 254
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 1 cut(s) 432
Vha464I CTTAAG 1 cut(s) 29
VpaK11BI GGWCC 3 cut(s) 173, 218, 525
XceI RCATGY 3 cut(s) 91, 193, 481
XspI CTAG 2 cut(s) 161, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.