Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0001474

Physical Location & Seq

Genomic Coordinates Forward (+)

4015282 .. 4020165

Gene Structure 4884 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0046000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2763 bp

ATGGCTATCTCTCAAGAACTCTACCCCTCCCAAGATGACCTCCTCTACGAAGAAGAGCTTCTCCGAAACCCTTACAGCCTCAAGCTCTGGTGGCGCTACCTAATCGCCCGATCCGACGCCCCCTTCAAGAAGCGCCGCACCATCTACGAGCGCGCCGTCAAGGCCCTCCCCGGAAGCTACAAGCTCTGGTACGCCTACCTCCGCGAGCGCCTCGAGCTCGTCCGCAACTTTCCGATCAACCACTCCGAGTACGAAACCCTAAACAACACCTTCGAGAGGGCGCTGGTCACAATGCACAAGATGCCGCGGATTTGGATTCTGTATCTGCAGAGCCTGACGGAGCAGAGATTGGTCACCAGGACTCGCCGGAGTTTTGACCGGGCGCTGTGTGCTCTTCCGGTGCAGCAGCACGACCGGATTTGGGAGCTGTATTTGGTGTTCGTGAGCCAGAAGGGGATCCCTATCCAGACCTCGCTTCGGGTCTACCGGAGGTACTTGCTTTATGACCCGACTCATGTTGAGGATTTTATTGCGTTTTTGATAGAGTCTGAGCTGTGGCAAGAGGCGGCGGAGCGGCTGGCCTCGGTGCTTAATGATGATCAGTTTAGGTCAATTAAGGGGAAGACGAAGCATAGGCTGTGGTTGGAGTTGTGTGATTTGCTTACTAAGAATGCCACGGCGGTTTCGGGGTTGAATGTGGATGCTATCATTAGAGGTGGGATTAAGAAGTTTACGGATGAGGTGGGGAGGCTGTGGACGTCGTTGGCGGATTATTATATTAAGAGGAGTTTGTTTGAGAAGGCTCGGGATGTGTTTGAGGAGGGTATGCAGACTGTGGTGACTGTGAGGGATTTCAGTGTGATTTTCGATGCGTATGCGCAGTTTGAAGAGAGTATGCTTGCGATTAAGATGGAGGCTTTGGGGAGTGATGAGGAGGAGGAGGAGGAGGAGGAAGGAGAGAATGGGAGAATGGAAGATGAGAGAAGTGAAGAGGAGGAGGAAGATGTCCGGACCAATGTAGACTTGTCTGTGGCTGAGCTTGAGAGGAAAATACTCCATGGGTTTTGGCTACATGATGAGAATGATGTTGATTTGAGGTTGGCTAGATTGGACCATCTTATGGATAGAAGACCCGAACTGGCTAATAGTGTTCTTTTGAGACAAAATCCGCATAATGTAGAGCAATGGCACCGGAGAGTGAAGCTGTTTGAGGGGAATCCTACAAAGCAGATACTTACTTACACCCAGGCTGTGAAGACAGTGGATCCAATGCAAGCAGTGGGGAAGCCTCATACGTTGTGGGTTGCTTTTGCTAAGCTGTATGAAACACATGGAGATATTGCAAATGCAAGAGTAATTTTTGATAAGGCCGTCCAGGTGAACTACAAGACAGTGGATAATCTGGCTAGTCTATGGTGTGAGTGGGCAGAAATGGAATTGCGGCATAAGAATTTTAAACGAGCCCTGGAACTGATGAGTAGAGCTACAGCAGAGCCGTCTGTTGAGGTCAAACGAAGAGTTGCTGCTGATGGGAATCCACCAGTTCAGATGAGGCTCCATAAATCCTTGAGGCTTTGGACCTTTTATGTGGACTTGGAGGAGAGCCTGGGTACACTAGAGTCTACTCGGGCAGTGTATGAGAGGATATTGGATTTGAGAATAGCCACACCTCAAATAATAATCAACTATGCATTGCTTCTGGAAGAACATAAATACTTTGAAGATGCATTCAAAGTCTATGAAAAGGGTACTCAAATATTCAAGTACCCGCATGTCAAAGACATATGGATTACATATCTCTCTAAATTTGTAAAAAGATATGGGAAGAATAAGCTCGAACGTGCAAGACTGCTCTTTGAGGATGCTGTTAAAGCGGCTCCTGCTGATGCGAAGAAACCTTTGTATCTTCAATTTGCAAAGCTGGAGGAGGATTATGGTCTAGCAAAGCGGGCTATGAAGGTCTATGATGAAGCAACTAAGGCTGTTCCAAACCATGAAAAGTTGAGCATGTATGAAATCTATATTGCCCGTGCAGCTGAGATATTTGGGATCCCCAAAACAAGGGAGATATATGAGCAAGCAATAGAATCTGGTCTCCCTGACAAAGATGTGAAGACAATGTGTCTAAAGTATGCTGAACTCGAGAAGAGCTTGGGCGAAATTGATCGTGCTCGGGGAGTTTATGTATTTGCATCTCAATTTTCAGATCCACGATCGGATGCAGAATTCTGGAACAAATGGCACGAGTTTGAGGTGCAACATGGGAATGAAGATACGTTCAGAGAAATGCTTCGAATCAAACGGAGTGTTTCTGCAAGCTATAGCCAGACACACTTTATTCTACCTGAGTATATGATGCAAAAGGACCAGAGGTTGAGCATTGATGAGGCAAAAGACAAATTGAAACAGGCTGGAGTCCCCGAAGATGAAATGGCTGCTCTTGAGAGGCAGTTGGCTCCTGCGATCAGCGATACACCCTCTAAAGACAGCAACAGAAAAGTGGGCTTTGTGAGTGCTGGAATAGAATCACAGACTGATGGAGGGATAAAAGTTGCTGCAAATCACGAAGACATTGAACTACCAGAAGAAAGTGATTCTGAAGATGAGGCGAATGTTGAAATTGCACAAAAGGAGGTCCCAGCTGCTGTTTTTGGAGATTTGGCTAACAAGAGAAAGGATTCTGAAGATAATGAGGGCGGAGACAAGGATGGTGAGAGCCGCCTTGGTGCTCTTGAGAGAATAAAGAGGCTGAAGAAAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624

Pfam Domains

Protein Families

UQ_con

Protein Analysis

920

Amino Acids

107.13

Weight (kDa)

5.6

Isoelectric Point (pI)

49.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_Syf1_CNRKL1_N	PF23233	12 - 170	1.3e-58	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N	PF23240	15 - 144	7.3e-09	PRP39 N-terminal HAT repeat
HAT_Syf1_M	PF23220	173 - 321	1.6e-58	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_M	PF23220	358 - 421	9.4e-24	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_CNRKL1_C	PF23231	423 - 817	2.8e-193	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_C	PF23241	521 - 587	7.4e-06	PRP39 C-terminal HAT repeat
Suf	PF05843	526 - 669	6.7e-06	Suppressor of forked protein (Suf)
HAT_PRP39_N	PF23240	562 - 660	3.5e-07	PRP39 N-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0001474)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08690 AT3G08690 AT3G08700 AT3G08700
fragaria_vesca	FvH4_3g11640 FvH4_6g09540 FvH4_7g15850
malus_domestica	MD01G1075300.v1.1 MD01G1214300.v1.1 MD04G1176600.v1.1 MD07G1144200.v1.1 MD12G1190900.v1.1
prunus_persica	Prupe.2G183100_v2.0.a1 Prupe.2G306300_v2.0.a1 Prupe.2G306300_v2.0.a1 Prupe.2G306300_v2.0.a1 Prupe.6G296100_v2.0.a1 Prupe.6G296100_v2.0.a1 Prupe.6G296100_v2.0.a1
pyrus_communis	pycom04g15580 pycom07g14260 pycom12g17820 pycom15g33780
rosa_chinensis	RchiOBHm_Chr1g0358671 RchiOBHm_Chr1g0380011 RchiOBHm_Chr3g0460141 RchiOBHm_Chr5g0018691
rosa_laevigata	RLG00000032387
rosa_multiflora	Rmu_co8206768.1_g000001 Rmu_sc0000449.1_g000050 Rmu_sc0001609.1_g000029 Rmu_sc0010911.1_g000002 Rmu_sc0025354.1_g000001
rosa_roxburghii	Rroxscaffold_1G00059080 Rroxscaffold_4G00279800 Rroxscaffold_4G00297720 Rroxscaffold_6G00419800
rosa_rugosa	Rorug01G0416400 Rorug01G0416500 Rorug03G0040400 Rorug04G0115100 Rorug05G0046000
rosa_samantha	Rh1AG441000 Rh1BG396700 Rh3DG102700 Rh5AG137000 Rh5BG135800 Rh5CG147700
rosa_wichuraiana	Rw1G038350 Rw3G008300 Rw5G012140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	761
Acc16I	TGCGCA	1 cut(s)	879
AccB1I	GGYRCC	1 cut(s)	1188
AccB7I	CCANNNNNTGG	1 cut(s)	1120
AccBSI	CCGCTC	1 cut(s)	574
AccI	GTMKAC	3 cut(s)	483, 1020, 1622
AccII	CGCG	3 cut(s)	153, 204, 307
AccIII	TCCGGA	1 cut(s)	1008
AclWI	GGATC	8 cut(s)	105, 451, 464, 1259, 1272, 2044, 2057, 2201
AcsI	RAATTY	3 cut(s)	1450, 1805, 2225
AcuI	CTGAAG	2 cut(s)	2619, 2703
AcyI	GRCGYC	2 cut(s)	117, 758
AfaI	GTAC	6 cut(s)	191, 251, 494, 1612, 1750, 1766
AfiI	CCNNNNNNNGG	5 cut(s)	276, 421, 477, 1120, 2061
AhdI	GACNNNNNGTC	1 cut(s)	2121
AjnI	CCWGG	5 cut(s)	356, 1245, 1374, 1464, 1605
Alw21I	GWGCWC	4 cut(s)	219, 394, 2173, 2731
Alw26I	GTCTC	3 cut(s)	1153, 2099, 2694
AlwI	GGATC	8 cut(s)	105, 451, 464, 1259, 1272, 2044, 2057, 2201
AlwNI	CAGNNNCTG	2 cut(s)	334, 2645
Ama87I	CYCGRG	5 cut(s)	212, 804, 1626, 2141, 2172
Aor13HI	TCCGGA	1 cut(s)	1008
AoxI	GGCC	3 cut(s)	162, 579, 1368
ApeKI	GCWGC	7 cut(s)	403, 406, 1523, 2033, 2435, 2555, 2642
ApoI	RAATTY	3 cut(s)	1450, 1805, 2225
Asp700I	GAANNNNTTC	3 cut(s)	57, 2289, 2677
AspLEI	GCGC	8 cut(s)	96, 135, 153, 155, 210, 283, 385, 880
AspS9I	GGNCC	6 cut(s)	163, 1011, 1111, 1578, 2365, 2635
AsuC2I	CCSGG	2 cut(s)	171, 380
AsuHPI	GGTGA	4 cut(s)	346, 850, 1390, 2723
AsuII	TTCGAA	1 cut(s)	2293
AvaI	CYCGRG	5 cut(s)	212, 804, 1626, 2141, 2172
AvaII	GGWCC	5 cut(s)	1011, 1111, 1578, 2365, 2635
BaeI	ACNNNNGTAYC	2 cut(s)	1886, 1919
BamHI	GGATCC	3 cut(s)	456, 1264, 2049
BanI	GGYRCC	1 cut(s)	1188
BanII	GRGCYC	2 cut(s)	219, 1465
BauI	CACGAG	1 cut(s)	2243
BbsI	GAAGAC	5 cut(s)	629, 1135, 1262, 2120, 2574
Bbv12I	GWGCWC	4 cut(s)	219, 394, 2173, 2731
BbvI	GCAGC	7 cut(s)	415, 418, 1510, 2045, 2422, 2542, 2629
BccI	CCATC	6 cut(s)	149, 904, 1122, 1523, 2531, 2702
BceAI	ACGGC	4 cut(s)	140, 693, 1355, 1480
BcgI	CGANNNNNNTGC	2 cut(s)	857, 891
BciT130I	CCWGG	5 cut(s)	358, 1247, 1376, 1466, 1607
BclI	TGATCA	1 cut(s)	598
BcnI	CCSGG	2 cut(s)	171, 380
BcoDI	GTCTC	3 cut(s)	1153, 2099, 2694
BfaI	CTAG	4 cut(s)	1104, 1407, 1616, 1940
BfmI	CTRYAG	3 cut(s)	326, 1485, 2320
BfoI	RGCGCY	5 cut(s)	97, 136, 211, 284, 386
BglI	GCCNNNNNGGC	1 cut(s)	161
BlpI	GCTNAGC	2 cut(s)	1035, 1314
Bme1390I	CCNGG	7 cut(s)	171, 358, 380, 1247, 1376, 1466, 1607
Bme18I	GGWCC	5 cut(s)	1011, 1111, 1578, 2365, 2635
BmeRI	GACNNNNNGTC	1 cut(s)	2121
BmeT110I	CYCGRG	5 cut(s)	212, 804, 1626, 2141, 2172
BmgT120I	GGNCC	6 cut(s)	163, 1011, 1111, 1578, 2365, 2635
BmiI	GGNNCC	8 cut(s)	458, 1190, 1266, 1556, 1878, 2051, 2457, 2637
BmrFI	CCNGG	7 cut(s)	171, 358, 380, 1247, 1376, 1466, 1607
BmsI	GCATC	9 cut(s)	291, 691, 859, 1714, 1852, 1876, 2201, 2209, 2346
BpiI	GAAGAC	5 cut(s)	629, 1135, 1262, 2120, 2574
BpmI	CTGGAG	2 cut(s)	1943, 2433
Bpu1102I	GCTNAGC	2 cut(s)	1035, 1314
Bpu14I	TTCGAA	1 cut(s)	2293
BpuEI	CTTGAG	5 cut(s)	65, 1061, 1588, 2462, 2753
BpuMI	CCSGG	2 cut(s)	171, 380
BsaBI	GATNNNNATC	2 cut(s)	461, 1533
BsaHI	GRCGYC	2 cut(s)	117, 758
BsaI	GGTCTC	1 cut(s)	2099
BsaJI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1057, 1245, 1464, 1606, 2722
BsaWI	WCCGGW	5 cut(s)	397, 414, 486, 1008, 1191
BsaXI	ACNNNNNCTCC	2 cut(s)	2169, 2199
Bsc4I	CCNNNNNNNGG	5 cut(s)	276, 421, 477, 1120, 2061
Bse1I	ACTGG	2 cut(s)	1143, 1541
Bse3DI	GCAATG	2 cut(s)	1190, 1691
Bse8I	GATNNNNATC	2 cut(s)	461, 1533
BseAI	TCCGGA	1 cut(s)	1008
BseBI	CCWGG	5 cut(s)	358, 1247, 1376, 1466, 1607
BseDI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1057, 1245, 1464, 1606, 2722
BseGI	GGATG	6 cut(s)	706, 742, 814, 1867, 2224, 2713
BseJI	GATNNNNATC	2 cut(s)	461, 1533
BseLI	CCNNNNNNNGG	5 cut(s)	276, 421, 477, 1120, 2061
BseMI	GCAATG	2 cut(s)	1190, 1691
BseMII	CTCAG	4 cut(s)	540, 1026, 2028, 2337
BseNI	ACTGG	2 cut(s)	1143, 1541
BsePI	GCGCGC	1 cut(s)	151
BseXI	GCAGC	7 cut(s)	415, 418, 1510, 2045, 2422, 2542, 2629
BseYI	CCCAGC	1 cut(s)	2638
BsgI	GTGCAG	2 cut(s)	422, 2052
Bsh1236I	CGCG	3 cut(s)	153, 204, 307
Bsh1285I	CGRYCG	2 cut(s)	415, 2216
BshFI	GGCC	3 cut(s)	164, 581, 1370
BshNI	GGYRCC	1 cut(s)	1188
BsiEI	CGRYCG	2 cut(s)	415, 2216
BsiHKAI	GWGCWC	4 cut(s)	219, 394, 2173, 2731
BsiHKCI	CYCGRG	5 cut(s)	212, 804, 1626, 2141, 2172
BsiSI	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1009, 1192
BslFI	GGGAC	2 cut(s)	2402, 2621
BslI	CCNNNNNNNGG	5 cut(s)	276, 421, 477, 1120, 2061
BsmAI	GTCTC	3 cut(s)	1153, 2099, 2694
BsmFI	GGGAC	2 cut(s)	2402, 2621
BsmI	GAATGC	2 cut(s)	676, 1727
BsnI	GGCC	3 cut(s)	164, 581, 1370
Bso31I	GGTCTC	1 cut(s)	2099
BsoBI	CYCGRG	5 cut(s)	212, 804, 1626, 2141, 2172
Bsp119I	TTCGAA	1 cut(s)	2293
Bsp1286I	GDGCHC	5 cut(s)	219, 394, 1465, 2173, 2731
Bsp13I	TCCGGA	1 cut(s)	1008
Bsp1720I	GCTNAGC	2 cut(s)	1035, 1314
Bsp19I	CCATGG	1 cut(s)	1057
BspANI	GGCC	3 cut(s)	164, 581, 1370
BspCNI	CTCAG	4 cut(s)	541, 1027, 2029, 2338
BspEI	TCCGGA	1 cut(s)	1008
BspFNI	CGCG	3 cut(s)	153, 204, 307
BspLI	GGNNCC	8 cut(s)	458, 1190, 1266, 1556, 1878, 2051, 2457, 2637
BspMAI	CTGCAG	1 cut(s)	330
BspPI	GGATC	8 cut(s)	105, 451, 464, 1259, 1272, 2044, 2057, 2201
BspQI	GCTCTTC	3 cut(s)	48, 399, 2141
BspT104I	TTCGAA	1 cut(s)	2293
BspT107I	GGYRCC	1 cut(s)	1188
BspTNI	GGTCTC	1 cut(s)	2099
BsrBI	CCGCTC	1 cut(s)	574
BsrDI	GCAATG	2 cut(s)	1190, 1691
BsrI	ACTGG	2 cut(s)	1143, 1541
BssECI	CCNNGG	9 cut(s)	169, 305, 582, 675, 1057, 1245, 1464, 1606, 2722
BssHII	GCGCGC	1 cut(s)	151
BssNI	GRCGYC	2 cut(s)	117, 758
BssSI	CACGAG	1 cut(s)	2243
BssT1I	CCWWGG	2 cut(s)	1057, 2722
Bst2BI	CACGAG	1 cut(s)	2243
Bst2UI	CCWGG	5 cut(s)	358, 1247, 1376, 1466, 1607
Bst4CI	ACNGT	4 cut(s)	835, 844, 1261, 1393
Bst6I	CTCTTC	6 cut(s)	48, 399, 882, 984, 1510, 2141
BstACI	GRCGYC	2 cut(s)	117, 758
BstAPI	GCANNNNNTGC	1 cut(s)	301
BstBI	TTCGAA	1 cut(s)	2293
BstC8I	GCNNGC	8 cut(s)	153, 206, 579, 900, 1275, 1950, 2079, 2317
BstDEI	CTNAG	7 cut(s)	549, 666, 1035, 1314, 1977, 2037, 2346
BstDSI	CCRYGG	3 cut(s)	305, 675, 1057
BstEII	GGTNACC	1 cut(s)	352
BstENI	CCTNNNNNAGG	1 cut(s)	274
BstF5I	GGATG	6 cut(s)	706, 742, 814, 1867, 2224, 2713
BstFNI	CGCG	3 cut(s)	153, 204, 307
BstH2I	RGCGCY	5 cut(s)	97, 136, 211, 284, 386
BstHHI	GCGC	8 cut(s)	96, 135, 153, 155, 210, 283, 385, 880
BstMAI	GTCTC	3 cut(s)	1153, 2099, 2694
BstMCI	CGRYCG	2 cut(s)	415, 2216
BstNI	CCWGG	5 cut(s)	358, 1247, 1376, 1466, 1607
BstNSI	RCATGY	2 cut(s)	1775, 2011
BstPI	GGTNACC	1 cut(s)	352
BstSCI	CCNGG	7 cut(s)	169, 356, 378, 1245, 1374, 1464, 1605
BstSFI	CTRYAG	3 cut(s)	326, 1485, 2320
BstUI	CGCG	3 cut(s)	153, 204, 307
BstV1I	GCAGC	7 cut(s)	415, 418, 1510, 2045, 2422, 2542, 2629
BstV2I	GAAGAC	5 cut(s)	629, 1135, 1262, 2120, 2574
BstX2I	RGATCY	4 cut(s)	456, 1264, 2049, 2206
BstYI	RGATCY	4 cut(s)	456, 1264, 2049, 2206
BsuRI	GGCC	3 cut(s)	164, 581, 1370
BtgI	CCRYGG	3 cut(s)	305, 675, 1057
BtsCI	GGATG	6 cut(s)	706, 742, 814, 1867, 2224, 2713
BtsI	GCAGTG	2 cut(s)	1284, 1638
BtsIMutI	CAGTG	5 cut(s)	862, 1266, 1284, 1398, 1638
Cac8I	GCNNGC	8 cut(s)	153, 206, 579, 900, 1275, 1950, 2079, 2317
CaiI	CAGNNNCTG	2 cut(s)	334, 2645
CfoI	GCGC	8 cut(s)	96, 135, 153, 155, 210, 283, 385, 880
Cfr13I	GGNCC	6 cut(s)	163, 1011, 1111, 1578, 2365, 2635
Cfr42I	CCGCGG	1 cut(s)	308
CseI	GACGC	1 cut(s)	125
Csp6I	GTAC	6 cut(s)	190, 250, 493, 1611, 1749, 1765
CviAII	CATG	8 cut(s)	515, 1058, 1073, 1331, 1772, 1994, 2008, 2261
CviQI	GTAC	6 cut(s)	190, 250, 493, 1611, 1749, 1765
DdeI	CTNAG	7 cut(s)	549, 666, 1035, 1314, 1977, 2037, 2346
DraI	TTTAAA	1 cut(s)	1456
DriI	GACNNNNNGTC	1 cut(s)	2121
Eam1104I	CTCTTC	6 cut(s)	48, 399, 882, 984, 1510, 2141
Eam1105I	GACNNNNNGTC	1 cut(s)	2121
EarI	CTCTTC	6 cut(s)	48, 399, 882, 984, 1510, 2141
EciI	GGCGGA	3 cut(s)	584, 782, 2712
Ecl136II	GAGCTC	1 cut(s)	217
Eco130I	CCWWGG	2 cut(s)	1057, 2722
Eco24I	GRGCYC	2 cut(s)	219, 1465
Eco31I	GGTCTC	1 cut(s)	2099
Eco47I	GGWCC	5 cut(s)	1011, 1111, 1578, 2365, 2635
Eco53kI	GAGCTC	1 cut(s)	217
Eco57I	CTGAAG	2 cut(s)	2619, 2703
Eco88I	CYCGRG	5 cut(s)	212, 804, 1626, 2141, 2172
Eco91I	GGTNACC	1 cut(s)	352
EcoICRI	GAGCTC	1 cut(s)	217
EcoNI	CCTNNNNNAGG	1 cut(s)	274
EcoO109I	RGGNCCY	2 cut(s)	163, 2635
EcoO65I	GGTNACC	1 cut(s)	352
EcoRI	GAATTC	1 cut(s)	2225
EcoRII	CCWGG	5 cut(s)	356, 1245, 1374, 1464, 1605
EcoT14I	CCWWGG	2 cut(s)	1057, 2722
EcoT22I	ATGCAT	2 cut(s)	1693, 1729
EcoT38I	GRGCYC	2 cut(s)	219, 1465
ErhI	CCWWGG	2 cut(s)	1057, 2722
FaeI	CATG	8 cut(s)	518, 1061, 1076, 1334, 1775, 1997, 2011, 2264
FalI	AAGNNNNNCTT	2 cut(s)	42, 74
FaqI	GGGAC	2 cut(s)	2402, 2621
FatI	CATG	8 cut(s)	514, 1057, 1072, 1330, 1771, 1993, 2007, 2260
FauI	CCCGC	2 cut(s)	1776, 1941
FauNDI	CATATG	1 cut(s)	1784
FbaI	TGATCA	1 cut(s)	598
FblI	GTMKAC	3 cut(s)	483, 1020, 1622
FokI	GGATG	6 cut(s)	713, 749, 821, 1874, 2231, 2720
FriOI	GRGCYC	2 cut(s)	219, 1465
FspBI	CTAG	4 cut(s)	1104, 1407, 1616, 1940
FspI	TGCGCA	1 cut(s)	879
GlaI	GCGC	8 cut(s)	95, 134, 152, 154, 209, 282, 384, 879
GsaI	CCCAGC	1 cut(s)	2642
GsuI	CTGGAG	2 cut(s)	1943, 2433
HaeII	RGCGCY	5 cut(s)	97, 136, 211, 284, 386
HaeIII	GGCC	3 cut(s)	164, 581, 1370
HapII	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1009, 1192
HgaI	GACGC	1 cut(s)	125
HhaI	GCGC	8 cut(s)	96, 135, 153, 155, 210, 283, 385, 880
Hin1I	GRCGYC	2 cut(s)	117, 758
Hin1II	CATG	8 cut(s)	518, 1061, 1076, 1334, 1775, 1997, 2011, 2264
Hin6I	GCGC	8 cut(s)	94, 133, 151, 153, 208, 281, 383, 878
HinP1I	GCGC	8 cut(s)	94, 133, 151, 153, 208, 281, 383, 878
HpaII	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1009, 1192
HphI	GGTGA	4 cut(s)	346, 850, 1390, 2723
Hpy166II	GTNNAC	8 cut(s)	484, 732, 756, 1021, 1381, 1591, 1613, 1623
Hpy8I	GTNNAC	8 cut(s)	484, 732, 756, 1021, 1381, 1591, 1613, 1623
Hpy99I	CGWCG	2 cut(s)	119, 763
HpyAV	CCTTC	6 cut(s)	133, 280, 445, 793, 947, 1951
HpyCH4III	ACNGT	4 cut(s)	835, 844, 1261, 1393
HpyCH4IV	ACGT	4 cut(s)	758, 1295, 1840, 2276
HpyF3I	CTNAG	7 cut(s)	549, 666, 1035, 1314, 1977, 2037, 2346
HpySE526I	ACGT	4 cut(s)	758, 1295, 1840, 2276
Hsp92I	GRCGYC	2 cut(s)	117, 758
Hsp92II	CATG	8 cut(s)	518, 1061, 1076, 1334, 1775, 1997, 2011, 2264
HspAI	GCGC	8 cut(s)	94, 133, 151, 153, 208, 281, 383, 878
Kpn2I	TCCGGA	1 cut(s)	1008
Ksp22I	TGATCA	1 cut(s)	598
KspI	CCGCGG	1 cut(s)	308
LguI	GCTCTTC	3 cut(s)	48, 399, 2141
LmnI	GCTCC	6 cut(s)	340, 424, 571, 1560, 1882, 2461
Lsp1109I	GCAGC	7 cut(s)	415, 418, 1510, 2045, 2422, 2542, 2629
LweI	GCATC	9 cut(s)	291, 691, 859, 1714, 1852, 1876, 2201, 2209, 2346
MaeI	CTAG	4 cut(s)	1104, 1407, 1616, 1940
MaeII	ACGT	4 cut(s)	758, 1295, 1840, 2276
MaeIII	GTNAC	3 cut(s)	286, 352, 838
MbiI	CCGCTC	1 cut(s)	574
MflI	RGATCY	4 cut(s)	456, 1264, 2049, 2206
MhlI	GDGCHC	5 cut(s)	219, 394, 1465, 2173, 2731
MlyI	GAGTC	5 cut(s)	355, 505, 554, 1628, 2424
MmeI	TCCRAC	2 cut(s)	138, 624
Mph1103I	ATGCAT	2 cut(s)	1693, 1729
MroI	TCCGGA	1 cut(s)	1008
MroXI	GAANNNNTTC	3 cut(s)	57, 2289, 2677
MseI	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1455, 1869
MslI	CAYNNNNRTG	1 cut(s)	2265
MspA1I	CMGCKG	3 cut(s)	307, 2036, 2642
MspI	CCGG	8 cut(s)	171, 367, 379, 398, 415, 487, 1009, 1192
MspR9I	CCNGG	7 cut(s)	171, 358, 380, 1247, 1376, 1466, 1607
Mva1269I	GAATGC	2 cut(s)	676, 1727
MvaI	CCWGG	5 cut(s)	358, 1247, 1376, 1466, 1607
MvnI	CGCG	3 cut(s)	153, 204, 307
NciI	CCSGG	2 cut(s)	171, 380
NcoI	CCATGG	1 cut(s)	1057
NdeI	CATATG	1 cut(s)	1784
NlaIII	CATG	8 cut(s)	518, 1061, 1076, 1334, 1775, 1997, 2011, 2264
NlaIV	GGNNCC	8 cut(s)	458, 1190, 1266, 1556, 1878, 2051, 2457, 2637
NmuCI	GTSAC	3 cut(s)	286, 352, 838
NsbI	TGCGCA	1 cut(s)	879
NsiI	ATGCAT	2 cut(s)	1693, 1729
NspI	RCATGY	2 cut(s)	1775, 2011
NspV	TTCGAA	1 cut(s)	2293
PaeR7I	CTCGAG	2 cut(s)	212, 2141
PauI	GCGCGC	1 cut(s)	151
PciSI	GCTCTTC	3 cut(s)	48, 399, 2141
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 153
PctI	GAATGC	2 cut(s)	676, 1727
PdmI	GAANNNNTTC	3 cut(s)	57, 2289, 2677
PfeI	GAWTC	8 cut(s)	316, 1216, 1534, 2087, 2295, 2525, 2594, 2678
PflMI	CCANNNNNTGG	1 cut(s)	1120
Ple19I	CGATCG	1 cut(s)	2216
PleI	GAGTC	5 cut(s)	355, 505, 553, 1627, 2423
PpsI	GAGTC	5 cut(s)	355, 505, 553, 1627, 2423
PpuMI	RGGWCCY	1 cut(s)	2635
Psp124BI	GAGCTC	1 cut(s)	219
Psp5II	RGGWCCY	1 cut(s)	2635
Psp6I	CCWGG	5 cut(s)	356, 1245, 1374, 1464, 1605
PspEI	GGTNACC	1 cut(s)	352
PspFI	CCCAGC	1 cut(s)	2638
PspGI	CCWGG	5 cut(s)	356, 1245, 1374, 1464, 1605
PspN4I	GGNNCC	8 cut(s)	458, 1190, 1266, 1556, 1878, 2051, 2457, 2637
PspPI	GGNCC	6 cut(s)	163, 1011, 1111, 1578, 2365, 2635
PspPPI	RGGWCCY	1 cut(s)	2635
PspXI	VCTCGAGB	1 cut(s)	212
PsrI	GAACNNNNNNTAC	2 cut(s)	422, 454
PstI	CTGCAG	1 cut(s)	330
PstNI	CAGNNNCTG	2 cut(s)	334, 2645
PsuI	RGATCY	4 cut(s)	456, 1264, 2049, 2206
PteI	GCGCGC	1 cut(s)	151
PvuI	CGATCG	1 cut(s)	2216
PvuII	CAGCTG	2 cut(s)	2036, 2642
RsaI	GTAC	6 cut(s)	191, 251, 494, 1612, 1750, 1766
RsaNI	GTAC	6 cut(s)	190, 250, 493, 1611, 1749, 1765
RseI	CAYNNNNRTG	1 cut(s)	2265
SacI	GAGCTC	1 cut(s)	219
SacII	CCGCGG	1 cut(s)	308
SapI	GCTCTTC	3 cut(s)	48, 399, 2141
SaqAI	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1455, 1869
Sau96I	GGNCC	6 cut(s)	163, 1011, 1111, 1578, 2365, 2635
SchI	GAGTC	5 cut(s)	355, 505, 554, 1628, 2424
ScrFI	CCNGG	7 cut(s)	171, 358, 380, 1247, 1376, 1466, 1607
SduI	GDGCHC	5 cut(s)	219, 394, 1465, 2173, 2731
SfaNI	GCATC	9 cut(s)	291, 691, 859, 1714, 1852, 1876, 2201, 2209, 2346
SfcI	CTRYAG	3 cut(s)	326, 1485, 2320
Sfr274I	CTCGAG	2 cut(s)	212, 2141
Sfr303I	CCGCGG	1 cut(s)	308
SfuI	TTCGAA	1 cut(s)	2293
SgrBI	CCGCGG	1 cut(s)	308
SinI	GGWCC	5 cut(s)	1011, 1111, 1578, 2365, 2635
SlaI	CTCGAG	2 cut(s)	212, 2141
SmiMI	CAYNNNNRTG	1 cut(s)	2265
SmlI	CTYRAG	8 cut(s)	12, 80, 212, 1040, 1567, 2141, 2441, 2732
SmoI	CTYRAG	8 cut(s)	12, 80, 212, 1040, 1567, 2141, 2441, 2732
SspI	AATATT	1 cut(s)	1758
SspMI	CTAG	4 cut(s)	1104, 1407, 1616, 1940
SstI	GAGCTC	1 cut(s)	219
StyD4I	CCNGG	7 cut(s)	169, 356, 378, 1245, 1374, 1464, 1605
StyI	CCWWGG	2 cut(s)	1057, 2722
TaaI	ACNGT	4 cut(s)	835, 844, 1261, 1393
TaiI	ACGT	4 cut(s)	761, 1298, 1843, 2279
TaqI	TCGA	6 cut(s)	213, 273, 867, 1836, 2142, 2293
TauI	GCSGC	7 cut(s)	138, 307, 569, 577, 1444, 1877, 2721
TfiI	GAWTC	8 cut(s)	316, 1216, 1534, 2087, 2295, 2525, 2594, 2678
Tru1I	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1455, 1869
Tru9I	TTAA	7 cut(s)	591, 615, 723, 780, 906, 1455, 1869
TscAI	CASTG	5 cut(s)	862, 1266, 1284, 1398, 1638
TseFI	GTSAC	3 cut(s)	286, 352, 838
TseI	GCWGC	7 cut(s)	403, 406, 1523, 2033, 2435, 2555, 2642
Tsp45I	GTSAC	3 cut(s)	286, 352, 838
TspDTI	ATGAA	8 cut(s)	1338, 1755, 1970, 1983, 2010, 2028, 2283, 2442
TspGWI	ACGGA	3 cut(s)	353, 749, 2317
TspRI	CASTG	5 cut(s)	862, 1266, 1284, 1398, 1638
Van91I	CCANNNNNTGG	1 cut(s)	1120
VpaK11BI	GGWCC	5 cut(s)	1011, 1111, 1578, 2365, 2635
XagI	CCTNNNNNAGG	1 cut(s)	274
XapI	RAATTY	3 cut(s)	1450, 1805, 2225
XceI	RCATGY	2 cut(s)	1775, 2011
XhoI	CTCGAG	2 cut(s)	212, 2141
XmiI	GTMKAC	3 cut(s)	483, 1020, 1622
XmnI	GAANNNNTTC	3 cut(s)	57, 2289, 2677
XspI	CTAG	4 cut(s)	1104, 1407, 1616, 1940
ZraI	GACGTC	1 cut(s)	759
Zsp2I	ATGCAT	2 cut(s)	1693, 1729

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.