Rorug04G0115100

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001474
Physical Location & Seq
Forward (+)
18119538 .. 18121221
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0115100.1

Sequence Viewer

Length: 723 bp
ATGTCGGCGGCGGCGGAGGAGTCAAGTGAGGCAGGCACCATGGGCGAAGAGGTTTTGAAGCTCAAAGAGAAGCTTTCTCAATGCCGACTCGAAATGGAGCGGCTCCTGACAGAGAAAAAGAGATGGAAGATCAAGAAGAAACTTCTCATGCGGGGTATAGAAAAACTAGAAGACAAGAATAGTAAAGAACAAGAAGTGTTTCTGTGTGTAGGATCTGAAAATTATGAGAGACAGATTACACTCTACAAGAACTTGAACACTACGAACCACAGTATGTGGCAGCTAGGGGACGAATTGTCCAAAGTAGATGACGAATTGGCCAAACTAGACGAGGAACAGTACAAGTTTTTTGATAGTGAAAGGTCTTGTGTGGGAACAGAAGTGGAGAAGAAAATAAGGCGGTCACAATCAAAGACAGAGAAGCTGGATAAGCTATACTCAGAGATGAATAAGAAACGGGACGATTTATTAGCTTTTCTTGAAACTCGTACAAAAAATGCAGAAATCACCGATGATGTGAAGGCTCTGGTGAGAGACCACTATACTCAATATATTGACACACTCAAGACTGATGAGGACGAGTATGCACTGAAAACAAAGATTATGGATATCATTCTACCACCGGGTTCTTCTCAGAAGCAAATTGGGATGTTTGCCAGGGTTGATTCTATATCTCCAATCTCTGCTGGGATTTCAAAGATTTTGAACTTTCTAAGAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

240

Amino Acids

27.88

Weight (kDa)

6.28

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 35
AccBSI CCGCTC 1 cut(s) 100
AciI CCGC 6 cut(s) 8, 11, 14, 100, 151, 400
AclWI GGATC 1 cut(s) 220
AcoI YGGCCR 1 cut(s) 318
AfaI GTAC 2 cut(s) 341, 490
AgsI TTSAA 5 cut(s) 58, 256, 482, 696, 706
AhdI GACNNNNNGTC 1 cut(s) 295
AjnI CCWGG 1 cut(s) 656
AluBI AGCT 6 cut(s) 61, 73, 283, 424, 433, 473
AluI AGCT 6 cut(s) 61, 73, 283, 424, 433, 473
Alw26I GTCTC 2 cut(s) 223, 528
AlwI GGATC 1 cut(s) 220
AoxI GGCC 1 cut(s) 318
ApeKI GCWGC 1 cut(s) 280
Asp700I GAANNNNTTC 1 cut(s) 198
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 2 cut(s) 499, 541
BalI TGGCCA 1 cut(s) 320
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 177
BbvI GCAGC 1 cut(s) 292
BccI CCATC 1 cut(s) 117
BciT130I CCWGG 1 cut(s) 658
BcnI CCSGG 1 cut(s) 624
BcoDI GTCTC 2 cut(s) 223, 528
BfaI CTAG 3 cut(s) 167, 284, 326
BisI GCNGC 4 cut(s) 9, 12, 101, 281
BlsI GCNGC 4 cut(s) 10, 13, 102, 282
Bme1390I CCNGG 2 cut(s) 624, 658
BmeRI GACNNNNNGTC 1 cut(s) 295
BmiI GGNNCC 2 cut(s) 37, 104
BmrFI CCNGG 2 cut(s) 624, 658
BpiI GAAGAC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 39, 657
BseBI CCWGG 1 cut(s) 658
BseDI CCNNGG 2 cut(s) 39, 657
BseGI GGATG 1 cut(s) 654
BseMII CTCAG 2 cut(s) 453, 647
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 292
BseYI CCCAGC 1 cut(s) 686
BshFI GGCC 1 cut(s) 320
BshNI GGYRCC 1 cut(s) 35
BsiSI CCGG 1 cut(s) 623
BslFI GGGAC 2 cut(s) 302, 473
BsmAI GTCTC 2 cut(s) 223, 528
BsmFI GGGAC 2 cut(s) 302, 473
BsnI GGCC 1 cut(s) 320
Bso31I GGTCTC 1 cut(s) 528
Bsp143I GATC 2 cut(s) 129, 212
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 6 cut(s) 8, 11, 14, 100, 151, 400
BspANI GGCC 1 cut(s) 320
BspCNI CTCAG 2 cut(s) 452, 646
BspLI GGNNCC 2 cut(s) 37, 104
BspPI GGATC 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 528
BsrBI CCGCTC 1 cut(s) 100
BssECI CCNNGG 2 cut(s) 39, 657
BssMI GATC 2 cut(s) 129, 212
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 658
Bst4CI ACNGT 2 cut(s) 272, 339
Bst6I CTCTTC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 3 cut(s) 439, 633, 713
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 1 cut(s) 654
BstKTI GATC 2 cut(s) 132, 215
BstMAI GTCTC 2 cut(s) 223, 528
BstMBI GATC 2 cut(s) 129, 212
BstMWI GCNNNNNNNGC 2 cut(s) 42, 430
BstNI CCWGG 1 cut(s) 658
BstSCI CCNGG 2 cut(s) 622, 656
BstV1I GCAGC 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 212
BstYI RGATCY 1 cut(s) 212
BsuRI GGCC 1 cut(s) 320
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 654
BtsIMutI CAGTG 1 cut(s) 587
Cac8I GCNNGC 1 cut(s) 34
Csp6I GTAC 2 cut(s) 340, 489
CviAII CATG 2 cut(s) 40, 148
CviJI RGCY 9 cut(s) 61, 73, 103, 283, 320, 424, 433, 473, 524
CviKI_1 RGCY 9 cut(s) 61, 73, 103, 283, 320, 424, 433, 473, 524
CviQI GTAC 2 cut(s) 340, 489
DdeI CTNAG 3 cut(s) 439, 633, 713
DpnI GATC 2 cut(s) 131, 214
DpnII GATC 2 cut(s) 129, 212
DriI GACNNNNNGTC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 42
Eam1105I GACNNNNNGTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 42
EciI GGCGGA 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 528
Eco32I GATATC 1 cut(s) 610
EcoRII CCWGG 1 cut(s) 656
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 2 cut(s) 43, 151
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 2 cut(s) 302, 473
FatI CATG 2 cut(s) 39, 147
FauI CCCGC 1 cut(s) 144
Fnu4HI GCNGC 4 cut(s) 9, 12, 101, 281
FokI GGATG 1 cut(s) 661
Fsp4HI GCNGC 4 cut(s) 9, 12, 101, 281
FspBI CTAG 3 cut(s) 167, 284, 326
GluI GCNGC 4 cut(s) 9, 12, 101, 281
GsaI CCCAGC 1 cut(s) 690
HaeIII GGCC 1 cut(s) 320
HapII CCGG 1 cut(s) 623
Hin1II CATG 2 cut(s) 43, 151
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 3 cut(s) 20, 87, 665
HpaII CCGG 1 cut(s) 623
HphI GGTGA 2 cut(s) 499, 541
Hpy188I TCNGA 3 cut(s) 217, 442, 636
Hpy188III TCNNGA 4 cut(s) 106, 133, 479, 565
HpyAV CCTTC 1 cut(s) 514
HpyCH4III ACNGT 2 cut(s) 272, 339
HpyCH4V TGCA 2 cut(s) 500, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 430
HpyF3I CTNAG 3 cut(s) 439, 633, 713
Hsp92II CATG 2 cut(s) 43, 151
Kzo9I GATC 2 cut(s) 129, 212
LmnI GCTCC 2 cut(s) 97, 108
LpnPI CCDG 8 cut(s) 18, 119, 410, 512, 636, 643, 670, 672
Lsp1109I GCAGC 1 cut(s) 292
MaeI CTAG 3 cut(s) 167, 284, 326
MaeIII GTNAC 1 cut(s) 402
MalI GATC 2 cut(s) 131, 214
MbiI CCGCTC 1 cut(s) 100
MboI GATC 2 cut(s) 129, 212
MboII GAAGA 6 cut(s) 59, 139, 148, 182, 400, 621
MflI RGATCY 1 cut(s) 212
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 4 cut(s) 220, 293, 314, 642
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 2 cut(s) 29, 81
MnlI CCTC 5 cut(s) 10, 22, 43, 325, 568
Mox20I TGGCCA 1 cut(s) 320
MroXI GAANNNNTTC 1 cut(s) 198
MscI TGGCCA 1 cut(s) 320
Msp20I TGGCCA 1 cut(s) 320
MspI CCGG 1 cut(s) 623
MspR9I CCNGG 2 cut(s) 624, 658
MvaI CCWGG 1 cut(s) 658
MwoI GCNNNNNNNGC 2 cut(s) 42, 430
NciI CCSGG 1 cut(s) 624
NcoI CCATGG 1 cut(s) 39
NdeII GATC 2 cut(s) 129, 212
NlaIII CATG 2 cut(s) 43, 151
NlaIV GGNNCC 2 cut(s) 37, 104
NmuCI GTSAC 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 665
PkrI GCNGC 4 cut(s) 10, 13, 102, 282
PleI GAGTC 2 cut(s) 28, 81
PpsI GAGTC 2 cut(s) 28, 81
Psp6I CCWGG 1 cut(s) 656
PspFI CCCAGC 1 cut(s) 686
PspGI CCWGG 1 cut(s) 656
PspN4I GGNNCC 2 cut(s) 37, 104
PsuI RGATCY 1 cut(s) 212
RsaI GTAC 2 cut(s) 341, 490
RsaNI GTAC 2 cut(s) 340, 489
SatI GCNGC 4 cut(s) 9, 12, 101, 281
Sau3AI GATC 2 cut(s) 129, 212
SchI GAGTC 2 cut(s) 29, 81
ScrFI CCNGG 2 cut(s) 624, 658
SetI ASST 8 cut(s) 54, 63, 75, 285, 365, 426, 435, 475
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 4 cut(s) 220, 293, 314, 642
SsiI CCGC 6 cut(s) 8, 11, 14, 100, 151, 400
SspMI CTAG 3 cut(s) 167, 284, 326
StyD4I CCNGG 2 cut(s) 622, 656
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 2 cut(s) 272, 339
TaqI TCGA 1 cut(s) 90
TasI AATT 4 cut(s) 220, 293, 314, 642
TatI WGTACW 1 cut(s) 339
TauI GCSGC 3 cut(s) 11, 14, 103
TfiI GAWTC 1 cut(s) 665
TscAI CASTG 1 cut(s) 594
TseFI GTSAC 1 cut(s) 402
TseI GCWGC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 402
TspDTI ATGAA 1 cut(s) 461
TspRI CASTG 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 3 cut(s) 167, 284, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.