pycom07g14260

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001474
Physical Location & Seq
Forward (+)
16204054 .. 16206620
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g14260.4

Sequence Viewer

Length: 453 bp
ATGGCTTCCAAACGGATCTTGAAGGAGCTTAAAGATCTCCAGAAAGACCCCCCGACCTCTTGCAGCGCAGGGCCTGTTGCTGAGGACATGTTCCATTGGCAAGCAACGATAATGGGTCCCCCAGATAGTCCTTATGCAGGGGGTGTCTTTCTAGTTACCATCCATTTCCCTCCCGATTATCCATTCAAACCACCTAAGGTTGCCTTTAGGACTAAGGTCTTTCATCCAAATATCAACAGCAATGGAAGTATCTGCCTTGACATTTTGAAAGAGCAGTGGAGCCCGGCTCTCACCATATCTAAGGTGTTGCTATCCATCTGTTCCCTGTTGACGGACCCAAACCCAGATGATCCCTTGAATGGAGTGCCGGAAATTGCTCATATGTACAAGACAGACAGGTCAAAGTACGAGACAACCGCAAGGAGCTGGACCCAGAAGTATGCTATGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

151

Amino Acids

16.72

Weight (kDa)

7.72

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 397
AciI CCGC 1 cut(s) 417
AclWI GGATC 2 cut(s) 23, 344
AfaI GTAC 2 cut(s) 386, 407
AfiI CCNNNNNNNGG 3 cut(s) 137, 331, 359
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 4 cut(s) 22, 187, 268, 358
AluBI AGCT 2 cut(s) 28, 426
AluI AGCT 2 cut(s) 28, 426
Alw26I GTCTC 1 cut(s) 404
AlwI GGATC 2 cut(s) 23, 344
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 4 cut(s) 71, 116, 334, 429
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 283
AvaII GGWCC 3 cut(s) 116, 334, 429
AxyI CCTNAGG 1 cut(s) 195
BanII GRGCYC 1 cut(s) 284
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 75
BccI CCATC 2 cut(s) 167, 323
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 2 cut(s) 152, 451
BglII AGATCT 1 cut(s) 34
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 3 cut(s) 116, 334, 429
BmgT120I GGNCC 4 cut(s) 71, 116, 334, 429
BmiI GGNNCC 5 cut(s) 117, 118, 281, 336, 431
BmrFI CCNGG 1 cut(s) 284
BoxI GACNNNNGTC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 271, 303
BpmI CTGGAG 1 cut(s) 23
Bpu10I CCTNAGC 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 284
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 331, 359
Bse21I CCTNAGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 247
BseGI GGATG 2 cut(s) 159, 223
BseLI CCNNNNNNNGG 3 cut(s) 137, 331, 359
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 72
BseXI GCAGC 1 cut(s) 75
BshFI GGCC 1 cut(s) 73
BsiSI CCGG 2 cut(s) 284, 368
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 3 cut(s) 137, 331, 359
BsmAI GTCTC 1 cut(s) 404
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 284
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 3 cut(s) 15, 34, 349
BspACI CCGC 1 cut(s) 417
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 73
BspLI GGNNCC 5 cut(s) 117, 118, 281, 336, 431
BspPI GGATC 2 cut(s) 23, 344
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 384
BssMI GATC 3 cut(s) 15, 34, 349
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 4 cut(s) 81, 195, 213, 300
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 159, 223
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 3 cut(s) 18, 37, 352
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 3 cut(s) 15, 34, 349
BstNSI RCATGY 1 cut(s) 91
BstPAI GACNNNNGTC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 75
BstX2I RGATCY 2 cut(s) 15, 34
BstYI RGATCY 2 cut(s) 15, 34
Bsu36I CCTNAGG 1 cut(s) 195
BsuRI GGCC 1 cut(s) 73
BtsCI GGATG 2 cut(s) 159, 223
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 4 cut(s) 71, 116, 334, 429
Csp6I GTAC 2 cut(s) 385, 406
CviAII CATG 1 cut(s) 88
CviJI RGCY 7 cut(s) 5, 28, 73, 282, 287, 426, 450
CviKI_1 RGCY 7 cut(s) 5, 28, 73, 282, 287, 426, 450
CviQI GTAC 2 cut(s) 385, 406
DdeI CTNAG 4 cut(s) 81, 195, 213, 300
DpnI GATC 3 cut(s) 17, 36, 351
DpnII GATC 3 cut(s) 15, 34, 349
DrdI GACNNNNNNGTC 1 cut(s) 397
DseDI GACNNNNNNGTC 1 cut(s) 397
Eco24I GRGCYC 1 cut(s) 284
Eco47I GGWCC 3 cut(s) 116, 334, 429
Eco81I CCTNAGG 1 cut(s) 195
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoO109I RGGNCCY 2 cut(s) 71, 116
EcoT38I GRGCYC 1 cut(s) 284
FaeI CATG 1 cut(s) 91
FaiI YATR 7 cut(s) 89, 135, 296, 381, 383, 441, 446
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FaqI GGGAC 1 cut(s) 102
FatI CATG 1 cut(s) 87
FauNDI CATATG 1 cut(s) 381
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 2 cut(s) 146, 210
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 2 cut(s) 152, 451
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 1 cut(s) 73
HapII CCGG 2 cut(s) 284, 368
HhaI GCGC 1 cut(s) 68
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HpaII CCGG 2 cut(s) 284, 368
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 330
Hpy188III TCNNGA 3 cut(s) 19, 40, 173
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 1 cut(s) 16
HpyCH4V TGCA 2 cut(s) 63, 137
HpyF3I CTNAG 4 cut(s) 81, 195, 213, 300
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 1 cut(s) 66
KflI GGGWCCC 1 cut(s) 116
Kzo9I GATC 3 cut(s) 15, 34, 349
LmnI GCTCC 3 cut(s) 25, 279, 423
Lsp1109I GCAGC 1 cut(s) 75
MaeI CTAG 2 cut(s) 152, 451
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 17, 36, 351
MboI GATC 3 cut(s) 15, 34, 349
MflI RGATCY 2 cut(s) 15, 34
MhlI GDGCHC 1 cut(s) 284
MluCI AATT 1 cut(s) 372
MnlI CCTC 3 cut(s) 67, 76, 180
MseI TTAA 1 cut(s) 30
MspI CCGG 2 cut(s) 284, 368
MspR9I CCNGG 1 cut(s) 284
NciI CCSGG 1 cut(s) 284
NdeI CATATG 1 cut(s) 381
NdeII GATC 3 cut(s) 15, 34, 349
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 5 cut(s) 117, 118, 281, 336, 431
NspI RCATGY 1 cut(s) 91
PciI ACATGT 1 cut(s) 87
PkrI GCNGC 1 cut(s) 65
PpuMI RGGWCCY 1 cut(s) 116
PscI ACATGT 1 cut(s) 87
PshAI GACNNNNGTC 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 116
PspN4I GGNNCC 5 cut(s) 117, 118, 281, 336, 431
PspPI GGNCC 4 cut(s) 71, 116, 334, 429
PspPPI RGGWCCY 1 cut(s) 116
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 2 cut(s) 15, 34
RsaI GTAC 2 cut(s) 386, 407
RsaNI GTAC 2 cut(s) 385, 406
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 3 cut(s) 15, 34, 349
Sau96I GGNCC 4 cut(s) 71, 116, 334, 429
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 1 cut(s) 284
SetI ASST 8 cut(s) 30, 59, 196, 201, 219, 306, 401, 428
SinI GGWCC 3 cut(s) 116, 334, 429
Sse9I AATT 1 cut(s) 372
SsiI CCGC 1 cut(s) 417
SspMI CTAG 2 cut(s) 152, 451
StyD4I CCNGG 1 cut(s) 282
TasI AATT 1 cut(s) 372
TatI WGTACW 1 cut(s) 384
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 1 cut(s) 281
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 1 cut(s) 212
TspGWI ACGGA 2 cut(s) 28, 347
TspRI CASTG 1 cut(s) 281
VpaK11BI GGWCC 3 cut(s) 116, 334, 429
XagI CCTNNNNNAGG 1 cut(s) 135
XceI RCATGY 1 cut(s) 91
XspI CTAG 2 cut(s) 152, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.