MD07G1144200.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001474
Physical Location & Seq
Forward (+)
21000848 .. 21003824
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1144200.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCTTCCAAACGGATCTTGAAGGAGCTCAAAGATCTCCAGAAAGACCCCCCGACCTCTTGCAGCGCAGGGCCTGTTGCTGAGGACATGTTCCATTGGCAAGCAACGATAATGGGTCCCCCAGATAGTCCTTATGCAGGGGGTGTCTTTCTAGTTACCATCCATTTCCCTCCCGATTATCCATTCAAACCACCTAAGGTTGCCTTTAGGACTAAGGTCTTTCATCCAAATATCAACAGCAATGGAAGTATCTGCCTTGACATTTTGAAAGAGCAGTGGAGCCCGGCTCTCACCATATCTAAGGTGTTGCTATCCATCTGTTCCCTGTTGACGGACCCAAACCCAGATGATCCCTTGGTGCCGGAAATTGCTCATATGTACAAGACAGACAGGTCAAAGTACGAGACAACCGCAAGGAGCTGGACCCAGAAGTATGCTATGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

149

Amino Acids

16.55

Weight (kDa)

7.72

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 3.7e-55 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 391
AccB1I GGYRCC 1 cut(s) 358
AciI CCGC 1 cut(s) 411
AclWI GGATC 2 cut(s) 23, 344
AfaI GTAC 2 cut(s) 380, 401
AfiI CCNNNNNNNGG 2 cut(s) 137, 331
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 3 cut(s) 22, 187, 268
AluBI AGCT 2 cut(s) 28, 420
AluI AGCT 2 cut(s) 28, 420
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 2 cut(s) 23, 344
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 4 cut(s) 71, 116, 334, 423
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 283
AvaII GGWCC 3 cut(s) 116, 334, 423
AxyI CCTNAGG 1 cut(s) 195
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 30, 284
Bbv12I GWGCWC 1 cut(s) 30
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 75
BccI CCATC 2 cut(s) 167, 323
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 2 cut(s) 152, 445
BglII AGATCT 1 cut(s) 34
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 3 cut(s) 116, 334, 423
BmgT120I GGNCC 4 cut(s) 71, 116, 334, 423
BmiI GGNNCC 6 cut(s) 117, 118, 281, 336, 360, 425
BmrFI CCNGG 1 cut(s) 284
BoxI GACNNNNGTC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 271, 303
BpmI CTGGAG 1 cut(s) 23
Bpu10I CCTNAGC 1 cut(s) 81
BpuMI CCSGG 1 cut(s) 284
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 331
Bse21I CCTNAGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 2 cut(s) 159, 223
BseLI CCNNNNNNNGG 2 cut(s) 137, 331
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 72
BseXI GCAGC 1 cut(s) 75
BshFI GGCC 1 cut(s) 73
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 2 cut(s) 284, 362
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 2 cut(s) 137, 331
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 30, 284
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 3 cut(s) 15, 34, 349
BspACI CCGC 1 cut(s) 411
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 73
BspLI GGNNCC 6 cut(s) 117, 118, 281, 336, 360, 425
BspPI GGATC 2 cut(s) 23, 344
BspT107I GGYRCC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 3 cut(s) 15, 34, 349
BssT1I CCWWGG 1 cut(s) 354
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 4 cut(s) 81, 195, 213, 300
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 159, 223
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 3 cut(s) 18, 37, 352
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 3 cut(s) 15, 34, 349
BstNSI RCATGY 1 cut(s) 91
BstPAI GACNNNNGTC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 75
BstX2I RGATCY 2 cut(s) 15, 34
BstYI RGATCY 2 cut(s) 15, 34
Bsu36I CCTNAGG 1 cut(s) 195
BsuRI GGCC 1 cut(s) 73
BtsCI GGATG 2 cut(s) 159, 223
BtsI GCAGTG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 4 cut(s) 71, 116, 334, 423
Csp6I GTAC 2 cut(s) 379, 400
CviAII CATG 1 cut(s) 88
CviJI RGCY 7 cut(s) 5, 28, 73, 282, 287, 420, 444
CviKI_1 RGCY 7 cut(s) 5, 28, 73, 282, 287, 420, 444
CviQI GTAC 2 cut(s) 379, 400
DdeI CTNAG 4 cut(s) 81, 195, 213, 300
DpnI GATC 3 cut(s) 17, 36, 351
DpnII GATC 3 cut(s) 15, 34, 349
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 2 cut(s) 30, 284
Eco47I GGWCC 3 cut(s) 116, 334, 423
Eco53kI GAGCTC 1 cut(s) 28
Eco81I CCTNAGG 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 28
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoO109I RGGNCCY 2 cut(s) 71, 116
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 2 cut(s) 30, 284
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 1 cut(s) 91
FaiI YATR 7 cut(s) 89, 135, 296, 375, 377, 435, 440
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FaqI GGGAC 1 cut(s) 102
FatI CATG 1 cut(s) 87
FauNDI CATATG 1 cut(s) 375
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 2 cut(s) 146, 210
FriOI GRGCYC 2 cut(s) 30, 284
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 2 cut(s) 152, 445
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 1 cut(s) 73
HapII CCGG 2 cut(s) 284, 362
HhaI GCGC 1 cut(s) 68
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HpaII CCGG 2 cut(s) 284, 362
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 330
Hpy188III TCNNGA 3 cut(s) 19, 40, 173
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 1 cut(s) 16
HpyCH4V TGCA 2 cut(s) 63, 137
HpyF3I CTNAG 4 cut(s) 81, 195, 213, 300
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 1 cut(s) 66
KflI GGGWCCC 1 cut(s) 116
Kzo9I GATC 3 cut(s) 15, 34, 349
LmnI GCTCC 3 cut(s) 25, 279, 417
Lsp1109I GCAGC 1 cut(s) 75
MaeI CTAG 2 cut(s) 152, 445
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 17, 36, 351
MboI GATC 3 cut(s) 15, 34, 349
MflI RGATCY 2 cut(s) 15, 34
MhlI GDGCHC 2 cut(s) 30, 284
MluCI AATT 1 cut(s) 366
MnlI CCTC 3 cut(s) 67, 76, 180
MspI CCGG 2 cut(s) 284, 362
MspR9I CCNGG 1 cut(s) 284
NciI CCSGG 1 cut(s) 284
NdeI CATATG 1 cut(s) 375
NdeII GATC 3 cut(s) 15, 34, 349
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 6 cut(s) 117, 118, 281, 336, 360, 425
NspI RCATGY 1 cut(s) 91
PciI ACATGT 1 cut(s) 87
PkrI GCNGC 1 cut(s) 65
PpuMI RGGWCCY 1 cut(s) 116
PscI ACATGT 1 cut(s) 87
PshAI GACNNNNGTC 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 116
PspN4I GGNNCC 6 cut(s) 117, 118, 281, 336, 360, 425
PspPI GGNCC 4 cut(s) 71, 116, 334, 423
PspPPI RGGWCCY 1 cut(s) 116
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 2 cut(s) 15, 34
RsaI GTAC 2 cut(s) 380, 401
RsaNI GTAC 2 cut(s) 379, 400
SacI GAGCTC 1 cut(s) 30
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 3 cut(s) 15, 34, 349
Sau96I GGNCC 4 cut(s) 71, 116, 334, 423
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 2 cut(s) 30, 284
SetI ASST 8 cut(s) 30, 59, 196, 201, 219, 306, 395, 422
SinI GGWCC 3 cut(s) 116, 334, 423
Sse9I AATT 1 cut(s) 366
SsiI CCGC 1 cut(s) 411
SspMI CTAG 2 cut(s) 152, 445
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 1 cut(s) 354
TasI AATT 1 cut(s) 366
TatI WGTACW 1 cut(s) 378
TscAI CASTG 1 cut(s) 281
TseI GCWGC 1 cut(s) 63
TspDTI ATGAA 1 cut(s) 212
TspGWI ACGGA 2 cut(s) 28, 347
TspRI CASTG 1 cut(s) 281
VpaK11BI GGWCC 3 cut(s) 116, 334, 423
XagI CCTNNNNNAGG 1 cut(s) 135
XceI RCATGY 1 cut(s) 91
XspI CTAG 2 cut(s) 152, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.