P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 168 |
| AfaI | GTAC | 1 cut(s) | 64 |
| AluBI | AGCT | 3 cut(s) | 29, 105, 225 |
| AluI | AGCT | 3 cut(s) | 29, 105, 225 |
| Alw21I | GWGCWC | 1 cut(s) | 127 |
| ApoI | RAATTY | 1 cut(s) | 168 |
| Bbv12I | GWGCWC | 1 cut(s) | 127 |
| BccI | CCATC | 1 cut(s) | 113 |
| BfaI | CTAG | 1 cut(s) | 222 |
| Bpu10I | CCTNAGC | 1 cut(s) | 177 |
| BpuEI | CTTGAG | 1 cut(s) | 91 |
| BseMII | CTCAG | 1 cut(s) | 191 |
| BsiHKAI | GWGCWC | 1 cut(s) | 127 |
| Bsp1286I | GDGCHC | 1 cut(s) | 127 |
| BspCNI | CTCAG | 1 cut(s) | 190 |
| Bst4CI | ACNGT | 1 cut(s) | 195 |
| BstC8I | GCNNGC | 2 cut(s) | 75, 103 |
| BstDEI | CTNAG | 1 cut(s) | 177 |
| BstNSI | RCATGY | 1 cut(s) | 77 |
| BtsIMutI | CAGTG | 1 cut(s) | 121 |
| Cac8I | GCNNGC | 2 cut(s) | 75, 103 |
| Csp6I | GTAC | 1 cut(s) | 63 |
| CviAII | CATG | 3 cut(s) | 74, 173, 185 |
| CviJI | RGCY | 3 cut(s) | 29, 105, 225 |
| CviKI_1 | RGCY | 3 cut(s) | 29, 105, 225 |
| CviQI | GTAC | 1 cut(s) | 63 |
| DdeI | CTNAG | 1 cut(s) | 177 |
| EcoRI | GAATTC | 1 cut(s) | 168 |
| FaeI | CATG | 3 cut(s) | 77, 176, 188 |
| FaiI | YATR | 4 cut(s) | 75, 174, 186, 213 |
| FatI | CATG | 3 cut(s) | 73, 172, 184 |
| FspBI | CTAG | 1 cut(s) | 222 |
| Hin1II | CATG | 3 cut(s) | 77, 176, 188 |
| HincII | GTYRAC | 1 cut(s) | 198 |
| HindII | GTYRAC | 1 cut(s) | 198 |
| HinfI | GANTC | 1 cut(s) | 111 |
| HpaI | GTTAAC | 1 cut(s) | 198 |
| Hpy166II | GTNNAC | 2 cut(s) | 65, 198 |
| Hpy188III | TCNNGA | 1 cut(s) | 108 |
| Hpy8I | GTNNAC | 2 cut(s) | 65, 198 |
| HpyCH4III | ACNGT | 1 cut(s) | 195 |
| HpyCH4V | TGCA | 2 cut(s) | 77, 101 |
| HpyF3I | CTNAG | 1 cut(s) | 177 |
| Hsp92II | CATG | 3 cut(s) | 77, 176, 188 |
| KspAI | GTTAAC | 1 cut(s) | 198 |
| LpnPI | CCDG | 1 cut(s) | 164 |
| MaeI | CTAG | 1 cut(s) | 222 |
| MaeIII | GTNAC | 1 cut(s) | 217 |
| MhlI | GDGCHC | 1 cut(s) | 127 |
| MluCI | AATT | 2 cut(s) | 18, 168 |
| MnlI | CCTC | 2 cut(s) | 47, 139 |
| MseI | TTAA | 3 cut(s) | 17, 21, 197 |
| MslI | CAYNNNNRTG | 2 cut(s) | 72, 78 |
| NlaIII | CATG | 3 cut(s) | 77, 176, 188 |
| NmuCI | GTSAC | 1 cut(s) | 217 |
| NspI | RCATGY | 1 cut(s) | 77 |
| PacI | TTAATTAA | 1 cut(s) | 21 |
| PaeI | GCATGC | 1 cut(s) | 77 |
| PfeI | GAWTC | 1 cut(s) | 111 |
| RsaI | GTAC | 1 cut(s) | 64 |
| RsaNI | GTAC | 1 cut(s) | 63 |
| RseI | CAYNNNNRTG | 2 cut(s) | 72, 78 |
| SaqAI | TTAA | 3 cut(s) | 17, 21, 197 |
| SduI | GDGCHC | 1 cut(s) | 127 |
| SetI | ASST | 4 cut(s) | 31, 107, 150, 227 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 72, 78 |
| SmlI | CTYRAG | 1 cut(s) | 106 |
| SmoI | CTYRAG | 1 cut(s) | 106 |
| SphI | GCATGC | 1 cut(s) | 77 |
| Sse9I | AATT | 2 cut(s) | 18, 168 |
| SspMI | CTAG | 1 cut(s) | 222 |
| TaaI | ACNGT | 1 cut(s) | 195 |
| TaqI | TCGA | 2 cut(s) | 127, 166 |
| TasI | AATT | 2 cut(s) | 18, 168 |
| TfiI | GAWTC | 1 cut(s) | 111 |
| Tru1I | TTAA | 3 cut(s) | 17, 21, 197 |
| Tru9I | TTAA | 3 cut(s) | 17, 21, 197 |
| TscAI | CASTG | 1 cut(s) | 121 |
| TseFI | GTSAC | 1 cut(s) | 217 |
| Tsp45I | GTSAC | 1 cut(s) | 217 |
| TspDTI | ATGAA | 2 cut(s) | 161, 228 |
| TspRI | CASTG | 1 cut(s) | 121 |
| XapI | RAATTY | 1 cut(s) | 168 |
| XceI | RCATGY | 1 cut(s) | 77 |
| XspI | CTAG | 1 cut(s) | 222 |