P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 303 |
| AatII | GACGTC | 1 cut(s) | 925 |
| Acc65I | GGTACC | 1 cut(s) | 830 |
| AccB1I | GGYRCC | 1 cut(s) | 830 |
| AccI | GTMKAC | 1 cut(s) | 925 |
| AccII | CGCG | 3 cut(s) | 119, 1122, 1748 |
| AciI | CCGC | 3 cut(s) | 199, 1046, 1287 |
| AclWI | GGATC | 5 cut(s) | 997, 1136, 1456, 1917, 2123 |
| AcoI | YGGCCR | 1 cut(s) | 196 |
| AcsI | RAATTY | 3 cut(s) | 957, 1111, 1501 |
| AcuI | CTGAAG | 1 cut(s) | 1731 |
| AcyI | GRCGYC | 1 cut(s) | 922 |
| AfaI | GTAC | 5 cut(s) | 481, 832, 1260, 1653, 1830 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1523 |
| AflII | CTTAAG | 2 cut(s) | 56, 1433 |
| AhlI | ACTAGT | 1 cut(s) | 1255 |
| AjiI | CACGTC | 1 cut(s) | 10 |
| AjnI | CCWGG | 1 cut(s) | 1074 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 666, 698, 1131, 1163 |
| Alw21I | GWGCWC | 3 cut(s) | 493, 1870, 1981 |
| Alw26I | GTCTC | 1 cut(s) | 1682 |
| Alw44I | GTGCAC | 1 cut(s) | 1866 |
| AlwI | GGATC | 5 cut(s) | 997, 1136, 1456, 1917, 2123 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 185 |
| Ama87I | CYCGRG | 2 cut(s) | 21, 2031 |
| AoxI | GGCC | 5 cut(s) | 190, 196, 598, 611, 930 |
| ApaLI | GTGCAC | 1 cut(s) | 1866 |
| ApoI | RAATTY | 3 cut(s) | 957, 1111, 1501 |
| AseI | ATTAAT | 1 cut(s) | 224 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1644 |
| Asp718I | GGTACC | 1 cut(s) | 830 |
| AspLEI | GCGC | 6 cut(s) | 48, 103, 119, 121, 358, 1748 |
| AspS9I | GGNCC | 5 cut(s) | 598, 611, 1733, 2036, 2134 |
| AsuHPI | GGTGA | 5 cut(s) | 17, 433, 698, 766, 2117 |
| AvaI | CYCGRG | 2 cut(s) | 21, 2031 |
| AvaII | GGWCC | 3 cut(s) | 1733, 2036, 2134 |
| BaeGI | GKGCMC | 1 cut(s) | 1870 |
| BanI | GGYRCC | 1 cut(s) | 830 |
| BanII | GRGCYC | 2 cut(s) | 493, 2151 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1855, 1887 |
| BbsI | GAAGAC | 1 cut(s) | 1899 |
| Bbv12I | GWGCWC | 3 cut(s) | 493, 1870, 1981 |
| BceAI | ACGGC | 1 cut(s) | 722 |
| BciT130I | CCWGG | 1 cut(s) | 1076 |
| BclI | TGATCA | 1 cut(s) | 1843 |
| BcoDI | GTCTC | 1 cut(s) | 1682 |
| BcuI | ACTAGT | 1 cut(s) | 1255 |
| BfaI | CTAG | 5 cut(s) | 696, 1256, 1323, 1610, 1784 |
| BfmI | CTRYAG | 2 cut(s) | 926, 2040 |
| BfrI | CTTAAG | 2 cut(s) | 56, 1433 |
| BglII | AGATCT | 1 cut(s) | 1426 |
| Bme1390I | CCNGG | 1 cut(s) | 1076 |
| Bme18I | GGWCC | 3 cut(s) | 1733, 2036, 2134 |
| BmeT110I | CYCGRG | 2 cut(s) | 21, 2031 |
| BmgBI | CACGTC | 1 cut(s) | 10 |
| BmgT120I | GGNCC | 5 cut(s) | 598, 611, 1733, 2036, 2134 |
| BmiI | GGNNCC | 2 cut(s) | 832, 2037 |
| BmrFI | CCNGG | 1 cut(s) | 1076 |
| BpiI | GAAGAC | 1 cut(s) | 1899 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 475, 507 |
| BpuEI | CTTGAG | 2 cut(s) | 119, 135 |
| Bsa29I | ATCGAT | 1 cut(s) | 270 |
| BsaHI | GRCGYC | 1 cut(s) | 922 |
| BsaJI | CCNNGG | 2 cut(s) | 193, 1736 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1523 |
| Bse1I | ACTGG | 3 cut(s) | 777, 1488, 1517 |
| Bse3DI | GCAATG | 3 cut(s) | 1636, 1641, 1884 |
| BseBI | CCWGG | 1 cut(s) | 1076 |
| BseCI | ATCGAT | 1 cut(s) | 270 |
| BseDI | CCNNGG | 2 cut(s) | 193, 1736 |
| BseGI | GGATG | 6 cut(s) | 349, 759, 897, 1278, 1365, 1606 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1523 |
| BseMI | GCAATG | 3 cut(s) | 1636, 1641, 1884 |
| BseMII | CTCAG | 3 cut(s) | 46, 223, 2069 |
| BseNI | ACTGG | 3 cut(s) | 777, 1488, 1517 |
| BsePI | GCGCGC | 1 cut(s) | 117 |
| BseRI | GAGGAG | 2 cut(s) | 499, 1430 |
| BseSI | GKGCMC | 1 cut(s) | 1870 |
| BseX3I | CGGCCG | 1 cut(s) | 196 |
| BsgI | GTGCAG | 3 cut(s) | 306, 1020, 2016 |
| Bsh1236I | CGCG | 3 cut(s) | 119, 1122, 1748 |
| Bsh1285I | CGRYCG | 1 cut(s) | 199 |
| BshFI | GGCC | 5 cut(s) | 192, 198, 600, 613, 932 |
| BshNI | GGYRCC | 1 cut(s) | 830 |
| BshVI | ATCGAT | 1 cut(s) | 270 |
| BsiEI | CGRYCG | 1 cut(s) | 199 |
| BsiHKAI | GWGCWC | 3 cut(s) | 493, 1870, 1981 |
| BsiHKCI | CYCGRG | 2 cut(s) | 21, 2031 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1523 |
| BsmAI | GTCTC | 1 cut(s) | 1682 |
| BsmI | GAATGC | 2 cut(s) | 800, 2022 |
| BsnI | GGCC | 5 cut(s) | 192, 198, 600, 613, 932 |
| BsoBI | CYCGRG | 2 cut(s) | 21, 2031 |
| Bsp1286I | GDGCHC | 4 cut(s) | 493, 1870, 1981, 2151 |
| Bsp1407I | TGTACA | 2 cut(s) | 1651, 1828 |
| BspACI | CCGC | 3 cut(s) | 199, 1046, 1287 |
| BspANI | GGCC | 5 cut(s) | 192, 198, 600, 613, 932 |
| BspCNI | CTCAG | 3 cut(s) | 45, 222, 2070 |
| BspDI | ATCGAT | 1 cut(s) | 270 |
| BspFNI | CGCG | 3 cut(s) | 119, 1122, 1748 |
| BspHI | TCATGA | 1 cut(s) | 1852 |
| BspLI | GGNNCC | 2 cut(s) | 832, 2037 |
| BspPI | GGATC | 5 cut(s) | 997, 1136, 1456, 1917, 2123 |
| BspQI | GCTCTTC | 1 cut(s) | 549 |
| BspT107I | GGYRCC | 1 cut(s) | 830 |
| BspTI | CTTAAG | 2 cut(s) | 56, 1433 |
| BsrDI | GCAATG | 3 cut(s) | 1636, 1641, 1884 |
| BsrGI | TGTACA | 2 cut(s) | 1651, 1828 |
| BsrI | ACTGG | 3 cut(s) | 777, 1488, 1517 |
| BssECI | CCNNGG | 2 cut(s) | 193, 1736 |
| BssHII | GCGCGC | 1 cut(s) | 117 |
| BssNI | GRCGYC | 1 cut(s) | 922 |
| BssT1I | CCWWGG | 1 cut(s) | 1736 |
| Bst2UI | CCWGG | 1 cut(s) | 1076 |
| Bst4CI | ACNGT | 5 cut(s) | 305, 421, 1793, 1934, 1976 |
| Bst6I | CTCTTC | 3 cut(s) | 549, 700, 1685 |
| BstACI | GRCGYC | 1 cut(s) | 922 |
| BstAFI | CTTAAG | 2 cut(s) | 56, 1433 |
| BstAUI | TGTACA | 2 cut(s) | 1651, 1828 |
| BstDEI | CTNAG | 4 cut(s) | 32, 209, 1192, 2078 |
| BstF5I | GGATG | 6 cut(s) | 349, 759, 897, 1278, 1365, 1606 |
| BstFNI | CGCG | 3 cut(s) | 119, 1122, 1748 |
| BstHHI | GCGC | 6 cut(s) | 48, 103, 119, 121, 358, 1748 |
| BstMAI | GTCTC | 1 cut(s) | 1682 |
| BstMCI | CGRYCG | 1 cut(s) | 199 |
| BstNI | CCWGG | 1 cut(s) | 1076 |
| BstNSI | RCATGY | 3 cut(s) | 285, 1532, 1835 |
| BstSCI | CCNGG | 1 cut(s) | 1074 |
| BstSFI | CTRYAG | 2 cut(s) | 926, 2040 |
| BstSLI | GKGCMC | 1 cut(s) | 1870 |
| BstUI | CGCG | 3 cut(s) | 119, 1122, 1748 |
| BstV2I | GAAGAC | 1 cut(s) | 1899 |
| BstX2I | RGATCY | 4 cut(s) | 1002, 1141, 1426, 1909 |
| BstYI | RGATCY | 4 cut(s) | 1002, 1141, 1426, 1909 |
| BstZI | CGGCCG | 1 cut(s) | 196 |
| Bsu15I | ATCGAT | 1 cut(s) | 270 |
| BsuRI | GGCC | 5 cut(s) | 192, 198, 600, 613, 932 |
| BsuTUI | ATCGAT | 1 cut(s) | 270 |
| BtrI | CACGTC | 1 cut(s) | 10 |
| BtsCI | GGATG | 6 cut(s) | 349, 759, 897, 1278, 1365, 1606 |
| BtsIMutI | CAGTG | 2 cut(s) | 784, 1939 |
| CaiI | CAGNNNCTG | 1 cut(s) | 185 |
| CciI | TCATGA | 1 cut(s) | 1852 |
| CfoI | GCGC | 6 cut(s) | 48, 103, 119, 121, 358, 1748 |
| Cfr13I | GGNCC | 5 cut(s) | 598, 611, 1733, 2036, 2134 |
| ClaI | ATCGAT | 1 cut(s) | 270 |
| Csp6I | GTAC | 5 cut(s) | 480, 831, 1259, 1652, 1829 |
| CviAII | CATG | 9 cut(s) | 282, 934, 1135, 1154, 1529, 1832, 1853, 1859, 2027 |
| CviQI | GTAC | 5 cut(s) | 480, 831, 1259, 1652, 1829 |
| DdeI | CTNAG | 4 cut(s) | 32, 209, 1192, 2078 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 303 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 303 |
| EaeI | YGGCCR | 1 cut(s) | 196 |
| EagI | CGGCCG | 1 cut(s) | 196 |
| Eam1104I | CTCTTC | 3 cut(s) | 549, 700, 1685 |
| EarI | CTCTTC | 3 cut(s) | 549, 700, 1685 |
| Ecl136II | GAGCTC | 1 cut(s) | 491 |
| EclXI | CGGCCG | 1 cut(s) | 196 |
| Eco130I | CCWWGG | 1 cut(s) | 1736 |
| Eco24I | GRGCYC | 2 cut(s) | 493, 2151 |
| Eco32I | GATATC | 1 cut(s) | 1068 |
| Eco47I | GGWCC | 3 cut(s) | 1733, 2036, 2134 |
| Eco52I | CGGCCG | 1 cut(s) | 196 |
| Eco53kI | GAGCTC | 1 cut(s) | 491 |
| Eco57I | CTGAAG | 1 cut(s) | 1731 |
| Eco88I | CYCGRG | 2 cut(s) | 21, 2031 |
| EcoICRI | GAGCTC | 1 cut(s) | 491 |
| EcoO109I | RGGNCCY | 3 cut(s) | 598, 2036, 2134 |
| EcoRII | CCWGG | 1 cut(s) | 1074 |
| EcoRV | GATATC | 1 cut(s) | 1068 |
| EcoT14I | CCWWGG | 1 cut(s) | 1736 |
| EcoT38I | GRGCYC | 2 cut(s) | 493, 2151 |
| ErhI | CCWWGG | 1 cut(s) | 1736 |
| FaeI | CATG | 9 cut(s) | 285, 937, 1138, 1157, 1532, 1835, 1856, 1862, 2030 |
| FatI | CATG | 9 cut(s) | 281, 933, 1134, 1153, 1528, 1831, 1852, 1858, 2026 |
| FauI | CCCGC | 2 cut(s) | 1039, 1280 |
| FauNDI | CATATG | 1 cut(s) | 496 |
| FbaI | TGATCA | 1 cut(s) | 1843 |
| FblI | GTMKAC | 1 cut(s) | 925 |
| FokI | GGATG | 6 cut(s) | 356, 746, 904, 1265, 1352, 1613 |
| FriOI | GRGCYC | 2 cut(s) | 493, 2151 |
| FspBI | CTAG | 5 cut(s) | 696, 1256, 1323, 1610, 1784 |
| GlaI | GCGC | 6 cut(s) | 47, 102, 118, 120, 357, 1747 |
| HaeIII | GGCC | 5 cut(s) | 192, 198, 600, 613, 932 |
| HhaI | GCGC | 6 cut(s) | 48, 103, 119, 121, 358, 1748 |
| Hin1I | GRCGYC | 1 cut(s) | 922 |
| Hin1II | CATG | 9 cut(s) | 285, 937, 1138, 1157, 1532, 1835, 1856, 1862, 2030 |
| Hin6I | GCGC | 6 cut(s) | 46, 101, 117, 119, 356, 1746 |
| HinP1I | GCGC | 6 cut(s) | 46, 101, 117, 119, 356, 1746 |
| HincII | GTYRAC | 3 cut(s) | 1558, 1699, 1930 |
| HindII | GTYRAC | 3 cut(s) | 1558, 1699, 1930 |
| HindIII | AAGCTT | 2 cut(s) | 635, 1436 |
| HinfI | GANTC | 6 cut(s) | 503, 1238, 1420, 1513, 1989, 2074 |
| HphI | GGTGA | 5 cut(s) | 17, 433, 698, 766, 2117 |
| Hpy166II | GTNNAC | 7 cut(s) | 926, 1558, 1699, 1733, 1868, 1930, 2090 |
| Hpy188I | TCNGA | 8 cut(s) | 35, 212, 309, 669, 943, 1283, 1711, 2079 |
| Hpy8I | GTNNAC | 7 cut(s) | 926, 1558, 1699, 1733, 1868, 1930, 2090 |
| HpyAV | CCTTC | 8 cut(s) | 87, 271, 436, 821, 1300, 1706, 1712, 2045 |
| HpyCH4III | ACNGT | 5 cut(s) | 305, 421, 1793, 1934, 1976 |
| HpyCH4IV | ACGT | 5 cut(s) | 9, 326, 628, 823, 922 |
| HpyF3I | CTNAG | 4 cut(s) | 32, 209, 1192, 2078 |
| HpySE526I | ACGT | 5 cut(s) | 9, 326, 628, 823, 922 |
| Hsp92I | GRCGYC | 1 cut(s) | 922 |
| Hsp92II | CATG | 9 cut(s) | 285, 937, 1138, 1157, 1532, 1835, 1856, 1862, 2030 |
| HspAI | GCGC | 6 cut(s) | 46, 101, 117, 119, 356, 1746 |
| KpnI | GGTACC | 1 cut(s) | 834 |
| Ksp22I | TGATCA | 1 cut(s) | 1843 |
| LguI | GCTCTTC | 1 cut(s) | 549 |
| LmnI | GCTCC | 6 cut(s) | 182, 366, 496, 556, 793, 943 |
| MaeI | CTAG | 5 cut(s) | 696, 1256, 1323, 1610, 1784 |
| MaeII | ACGT | 5 cut(s) | 9, 326, 628, 823, 922 |
| MaeIII | GTNAC | 8 cut(s) | 5, 76, 367, 620, 686, 1402, 1717, 1970 |
| MboII | GAAGA | 8 cut(s) | 498, 536, 563, 717, 953, 1151, 1672, 1904 |
| MfeI | CAATTG | 3 cut(s) | 897, 1146, 2142 |
| MflI | RGATCY | 4 cut(s) | 1002, 1141, 1426, 1909 |
| MhlI | GDGCHC | 4 cut(s) | 493, 1870, 1981, 2151 |
| MlyI | GAGTC | 1 cut(s) | 1983 |
| MmeI | TCCRAC | 3 cut(s) | 43, 274, 321 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1644 |
| MseI | TTAA | 8 cut(s) | 57, 224, 228, 411, 459, 741, 1434, 2060 |
| MslI | CAYNNNNRTG | 7 cut(s) | 846, 856, 1533, 1551, 1851, 1857, 1863 |
| MspA1I | CMGCKG | 1 cut(s) | 290 |
| MspCI | CTTAAG | 2 cut(s) | 56, 1433 |
| MspR9I | CCNGG | 1 cut(s) | 1076 |
| MunI | CAATTG | 3 cut(s) | 897, 1146, 2142 |
| Mva1269I | GAATGC | 2 cut(s) | 800, 2022 |
| MvaI | CCWGG | 1 cut(s) | 1076 |
| MvnI | CGCG | 3 cut(s) | 119, 1122, 1748 |
| NdeI | CATATG | 1 cut(s) | 496 |
| NlaIII | CATG | 9 cut(s) | 285, 937, 1138, 1157, 1532, 1835, 1856, 1862, 2030 |
| NlaIV | GGNNCC | 2 cut(s) | 832, 2037 |
| NmeAIII | GCCGAG | 1 cut(s) | 174 |
| NmuCI | GTSAC | 5 cut(s) | 5, 76, 620, 686, 1970 |
| NspI | RCATGY | 3 cut(s) | 285, 1532, 1835 |
| PacI | TTAATTAA | 1 cut(s) | 228 |
| PaeI | GCATGC | 1 cut(s) | 1532 |
| PagI | TCATGA | 1 cut(s) | 1852 |
| PauI | GCGCGC | 1 cut(s) | 117 |
| PciSI | GCTCTTC | 1 cut(s) | 549 |
| PctI | GAATGC | 2 cut(s) | 800, 2022 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1644 |
| PfeI | GAWTC | 5 cut(s) | 503, 1238, 1420, 1513, 2074 |
| PleI | GAGTC | 1 cut(s) | 1983 |
| PpsI | GAGTC | 1 cut(s) | 1983 |
| PpuMI | RGGWCCY | 2 cut(s) | 2036, 2134 |
| PshBI | ATTAAT | 1 cut(s) | 224 |
| Psp124BI | GAGCTC | 1 cut(s) | 493 |
| Psp5II | RGGWCCY | 2 cut(s) | 2036, 2134 |
| Psp6I | CCWGG | 1 cut(s) | 1074 |
| PspGI | CCWGG | 1 cut(s) | 1074 |
| PspN4I | GGNNCC | 2 cut(s) | 832, 2037 |
| PspPI | GGNCC | 5 cut(s) | 598, 611, 1733, 2036, 2134 |
| PspPPI | RGGWCCY | 2 cut(s) | 2036, 2134 |
| PstNI | CAGNNNCTG | 1 cut(s) | 185 |
| PsuI | RGATCY | 4 cut(s) | 1002, 1141, 1426, 1909 |
| PteI | GCGCGC | 1 cut(s) | 117 |
| PvuII | CAGCTG | 1 cut(s) | 290 |
| RsaI | GTAC | 5 cut(s) | 481, 832, 1260, 1653, 1830 |
| RsaNI | GTAC | 5 cut(s) | 480, 831, 1259, 1652, 1829 |
| RseI | CAYNNNNRTG | 7 cut(s) | 846, 856, 1533, 1551, 1851, 1857, 1863 |
| SacI | GAGCTC | 1 cut(s) | 493 |
| SapI | GCTCTTC | 1 cut(s) | 549 |
| SaqAI | TTAA | 8 cut(s) | 57, 224, 228, 411, 459, 741, 1434, 2060 |
| Sau96I | GGNCC | 5 cut(s) | 598, 611, 1733, 2036, 2134 |
| SchI | GAGTC | 1 cut(s) | 1983 |
| ScrFI | CCNGG | 1 cut(s) | 1076 |
| SduI | GDGCHC | 4 cut(s) | 493, 1870, 1981, 2151 |
| SfcI | CTRYAG | 2 cut(s) | 926, 2040 |
| SinI | GGWCC | 3 cut(s) | 1733, 2036, 2134 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 846, 856, 1533, 1551, 1851, 1857, 1863 |
| SmlI | CTYRAG | 4 cut(s) | 56, 134, 150, 1433 |
| SmoI | CTYRAG | 4 cut(s) | 56, 134, 150, 1433 |
| SpeI | ACTAGT | 1 cut(s) | 1255 |
| SphI | GCATGC | 1 cut(s) | 1532 |
| SsiI | CCGC | 3 cut(s) | 199, 1046, 1287 |
| SspI | AATATT | 1 cut(s) | 1759 |
| SspMI | CTAG | 5 cut(s) | 696, 1256, 1323, 1610, 1784 |
| SstI | GAGCTC | 1 cut(s) | 493 |
| StyD4I | CCNGG | 1 cut(s) | 1074 |
| StyI | CCWWGG | 1 cut(s) | 1736 |
| TaaI | ACNGT | 5 cut(s) | 305, 421, 1793, 1934, 1976 |
| TaiI | ACGT | 5 cut(s) | 12, 329, 631, 826, 925 |
| TaqI | TCGA | 2 cut(s) | 270, 1981 |
| TaqII | GACCGA | 1 cut(s) | 1184 |
| TatI | WGTACW | 3 cut(s) | 1258, 1651, 1828 |
| TauI | GCSGC | 1 cut(s) | 201 |
| TfiI | GAWTC | 5 cut(s) | 503, 1238, 1420, 1513, 2074 |
| Tru1I | TTAA | 8 cut(s) | 57, 224, 228, 411, 459, 741, 1434, 2060 |
| Tru9I | TTAA | 8 cut(s) | 57, 224, 228, 411, 459, 741, 1434, 2060 |
| TscAI | CASTG | 2 cut(s) | 784, 1939 |
| TseFI | GTSAC | 5 cut(s) | 5, 76, 620, 686, 1970 |
| Tsp45I | GTSAC | 5 cut(s) | 5, 76, 620, 686, 1970 |
| TspRI | CASTG | 2 cut(s) | 784, 1939 |
| Vha464I | CTTAAG | 2 cut(s) | 56, 1433 |
| VneI | GTGCAC | 1 cut(s) | 1866 |
| VpaK11BI | GGWCC | 3 cut(s) | 1733, 2036, 2134 |
| VspI | ATTAAT | 1 cut(s) | 224 |
| XapI | RAATTY | 3 cut(s) | 957, 1111, 1501 |
| XbaI | TCTAGA | 2 cut(s) | 695, 1783 |
| XceI | RCATGY | 3 cut(s) | 285, 1532, 1835 |
| XmiI | GTMKAC | 1 cut(s) | 925 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1644 |
| XspI | CTAG | 5 cut(s) | 696, 1256, 1323, 1610, 1784 |
| ZraI | GACGTC | 1 cut(s) | 923 |