RLG00000024656

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000993
Physical Location & Seq
Reverse (-)
37932394 .. 37939972
7579 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024656

Sequence Viewer

Length: 2154 bp
ATGGACACTGTGGATCCTACGCGAGCTTTTGTCAAGAATGTTAAGCGTATCATCGTCAAGGTTGGAACTGCTGTGGTCACTCGTGGTGATGGAAGACTGGCATTGGGTAGACTGGGATCTCTTTGTGAGCAGCTTAAAGATATAAATTCTCAGGGCTATGAGGTTATTTTGGTGACCTCAGGAGCTGTTGGGCTTGGCCGTCAAAGGCTGAGATACAGGAGATTGGCAAATAGCAGCTTTGCTGATCTCCAAAACCCACAAAATGATTTTGATGGAAAGGCTTGTGCAGCTGTTGGACAGAGTAGTCTCATGGCTCTTTATGATGCCATGTTCAGCCAGCTTGATGTGACTTCTTCTCAGCTTCTCGTGACAGATAGTGTTTTCAGGGATGAAGCTTTCAGAAAGCAGCTCTCTGAAACTGTGAAGTCCTTGTTAAAACTGAGGGTTATTCCCATATTCAACGAAAATGATGCAGTTAGTACTAGGAAGGCCCCTTATGAGGACTCTTCTGGTATATTTTGGGACAATGATAGTTTGGCTGGTCTATTGGCCCTGGAACTGAAGGCTGATCTTCTTGTGTTGCTAAGTGATGTAGAGGGTCTCTATAGTGGTCCTCCCACTGATCCAAAGTCAAAGTTAATCCGTACATACGTGAAAGAAAAACATCATGGAGAAATAACATTTGGAGACAAGTCAAGGGTGGGCAGGGGGGGTATGACTGCTAAAGTCAATGCTGCTGTGTGTGCCGCTTATGGAGGAATCCCTGTTGTTATCACTAGTGGCTATGCTAATGATAACATTATCAAAGTGCTTCAAGGAGAACGAATTGGTACTGTCTTTCATCAAGATGCACATTTGTGGACTCTGGTTAAAGAAGAAAGTGCACGTGACTTGGCTGTTTCAGCACGGGAATGTTCTAGAAAGCTTCGAGCTCTAAACTCTCAGGATAGGAGGCAAATATTGCTTGATGTAGCTGATGCCTTGGAGGCAAATGAAAGTGACATTTTAGCTGAAAATGAAGCTGATATTGCAGCTGCACAGGAAGCTGGATATGATGGGGCACTAATAGCTCGGTTGGCCCTAAAGCCTGGGAAGATTTCTGCACTTGCAAAATCTATGCGTCAACTTGCAGACATGGAAGAGCCCATTGGCCAGGTCTTGCAGAAAACAGAGGTTGCAGATGGACTTGTCTTAGAGAAAGTGACTTGTCCCTTGGGTGTTCTTTTGGTTGTTTTCGAGTCTCGACCTGATGCCCTTGTTCAGATAGCTTCATTAGCAATTCAAAGTCGGAATGGTCTTCTGCTTAAAGGTGGGAAAGAAGCCAAGAGGTCAAATGCTATCTTGCACAAGGTCATCACCAAAGCAATCCCAGATACTGTTGGTGGTGAGCTTATTGGACTTGTAACTTCAAGAGATGATATTCCTGATCTGCTGAAGCTTGACGATGTGATAGATCTTGTCATCCCTAGAGGCAGTAATAAACTCGTTTCTCAAATCAAGGAGTCGACAAAGATACCTGTTCTTGGTCATGCTGATGGGATCTGCCATGTTTATATTGATAAGTCAGCTAATTTGGAGATGGCAAAGCGCATTGTTCATGATGCAAAGACAGATTATCCTGCAGCCTGTAATGCAATGGAAACACTTCTTGTACACCAGGATTTGTTTAACAATGGTCAGCTTAATGAGATAATCACACAACTCCGAGAGTCAGGTGTTACTTTATATGGTGGACCACATGCTGCAGTTTTGTTAAATCTTTCAGAGACAAGCACATTGCATCACGAGTACAGTTCAATGGCTTGCACTGTTGAAATTGTAGATGATGTGCATGATGCAATTGACCACATTCATGTATATGGAAGTGGTCACACTGAATGCATTGTTACTGAAGATCATGAAGTTGCTGAACATTTCTTAAGCCAAGTTGACAGCGCTGCAGTATTTCACAATGCAAGTACACGATTCTGCGACGGAGCTCGCTTTGGTCTTGGTGCAGAGGTTGGGATAAGTACAAGCAGGATTCACGCTCGAGGTCCTGTAGGAGTTGAAGGACTGCTAACAACCAGATGGACTCTGAGGGGGAAAGGGCAGATTGTGGATGGTGATAATGGGGTCGAATACACTCACAAGAATTTACTTAAAAGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

718

Amino Acids

77.62

Weight (kDa)

5.81

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 15 - 260 6e-36 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 303
AccI GTMKAC 2 cut(s) 109, 1505
AccII CGCG 1 cut(s) 22
AciI CCGC 1 cut(s) 747
AclWI GGATC 5 cut(s) 8, 21, 124, 617, 1547
AcoI YGGCCR 2 cut(s) 196, 1150
AcsI RAATTY 2 cut(s) 145, 2134
AcuI CTGAAG 3 cut(s) 581, 1454, 1911
AcvI CACGTG 1 cut(s) 887
AfaI GTAC 7 cut(s) 481, 646, 832, 1653, 1790, 1960, 2014
AfeI AGCGCT 1 cut(s) 1936
AfiI CCNNNNNNNGG 2 cut(s) 499, 1523
AflII CTTAAG 1 cut(s) 1918
AgsI TTSAA 7 cut(s) 460, 815, 1283, 1410, 1797, 1814, 2051
AhlI ACTAGT 1 cut(s) 776
AjnI CCWGG 4 cut(s) 552, 1087, 1152, 1656
AjuI GAANNNNNNNTTGG 4 cut(s) 666, 698, 1131, 1163
AleI CACNNNNGTG 1 cut(s) 856
Alw21I GWGCWC 3 cut(s) 886, 934, 1981
Alw26I GTCTC 5 cut(s) 311, 605, 681, 1245, 1760
Alw44I GTGCAC 1 cut(s) 882
AlwI GGATC 5 cut(s) 8, 21, 124, 617, 1547
AlwNI CAGNNNCTG 2 cut(s) 185, 1376
Ama87I CYCGRG 1 cut(s) 2031
Aor51HI AGCGCT 1 cut(s) 1936
AoxI GGCC 5 cut(s) 196, 489, 549, 1077, 1150
ApaLI GTGCAC 1 cut(s) 882
ApoI RAATTY 2 cut(s) 145, 2134
Asp700I GAANNNNTTC 1 cut(s) 1644
AspLEI GCGC 2 cut(s) 1590, 1937
AspS9I GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
AsuHPI GGTGA 5 cut(s) 98, 184, 1348, 1397, 2117
AvaI CYCGRG 1 cut(s) 2031
AvaII GGWCC 3 cut(s) 611, 1733, 2036
AxyI CCTNAGG 1 cut(s) 178
BaeGI GKGCMC 2 cut(s) 886, 1063
BalI TGGCCA 1 cut(s) 1152
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 3 cut(s) 934, 1146, 1981
BauI CACGAG 3 cut(s) 81, 365, 1784
BbrPI CACGTG 1 cut(s) 887
BbsI GAAGAC 2 cut(s) 100, 1289
Bbv12I GWGCWC 3 cut(s) 886, 934, 1981
BccI CCATC 8 cut(s) 83, 266, 1049, 1175, 1529, 1573, 2064, 2096
BceAI ACGGC 1 cut(s) 183
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 4 cut(s) 554, 1089, 1154, 1658
BcoDI GTCTC 5 cut(s) 311, 605, 681, 1245, 1760
BcuI ACTAGT 1 cut(s) 776
BfaI CTAG 4 cut(s) 483, 777, 918, 1467
BfmI CTRYAG 5 cut(s) 604, 1620, 1743, 1938, 2040
BfoI RGCGCY 1 cut(s) 1938
BfrI CTTAAG 1 cut(s) 1918
BglI GCCNNNNNGGC 1 cut(s) 986
BglII AGATCT 1 cut(s) 1453
BmcAI AGTACT 1 cut(s) 481
Bme1390I CCNGG 4 cut(s) 554, 1089, 1154, 1658
Bme18I GGWCC 3 cut(s) 611, 1733, 2036
BmeT110I CYCGRG 1 cut(s) 2031
BmgT120I GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
BmiI GGNNCC 2 cut(s) 15, 492
BmrFI CCNGG 4 cut(s) 554, 1089, 1154, 1658
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 8 cut(s) 313, 460, 838, 967, 1240, 1591, 1789, 1825
BmuI ACTGGG 1 cut(s) 122
BpiI GAAGAC 2 cut(s) 100, 1289
BsaAI YACGTR 2 cut(s) 652, 887
BsaI GGTCTC 1 cut(s) 605
BsaJI CCNNGG 4 cut(s) 552, 981, 1088, 1212
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 1523
Bse1I ACTGG 2 cut(s) 102, 117
Bse21I CCTNAGG 1 cut(s) 178
Bse3DI GCAATG 2 cut(s) 1641, 1775
BseBI CCWGG 4 cut(s) 554, 1089, 1154, 1658
BseDI CCNNGG 4 cut(s) 552, 981, 1088, 1212
BseGI GGATG 3 cut(s) 394, 1461, 2107
BseLI CCNNNNNNNGG 2 cut(s) 499, 1523
BseMI GCAATG 2 cut(s) 1641, 1775
BseMII CTCAG 7 cut(s) 164, 192, 200, 371, 431, 956, 2069
BseNI ACTGG 2 cut(s) 102, 117
BseSI GKGCMC 2 cut(s) 886, 1063
BsgI GTGCAG 4 cut(s) 306, 1020, 1086, 2016
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 5 cut(s) 198, 491, 551, 1079, 1152
BsiHKAI GWGCWC 3 cut(s) 886, 934, 1981
BsiHKCI CYCGRG 1 cut(s) 2031
BslFI GGGAC 2 cut(s) 536, 1194
BslI CCNNNNNNNGG 2 cut(s) 499, 1523
BsmAI GTCTC 5 cut(s) 311, 605, 681, 1245, 1760
BsmFI GGGAC 2 cut(s) 536, 1194
BsmI GAATGC 1 cut(s) 1883
BsnI GGCC 5 cut(s) 198, 491, 551, 1079, 1152
Bso31I GGTCTC 1 cut(s) 605
BsoBI CYCGRG 1 cut(s) 2031
Bsp1286I GDGCHC 5 cut(s) 886, 934, 1063, 1146, 1981
Bsp1407I TGTACA 1 cut(s) 1651
Bsp143I GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 1894
BspACI CCGC 1 cut(s) 747
BspANI GGCC 5 cut(s) 198, 491, 551, 1079, 1152
BspCNI CTCAG 7 cut(s) 163, 191, 201, 370, 432, 955, 2070
BspFNI CGCG 1 cut(s) 22
BspHI TCATGA 2 cut(s) 1597, 1897
BspLI GGNNCC 2 cut(s) 15, 492
BspMAI CTGCAG 3 cut(s) 1624, 1747, 1942
BspPI GGATC 5 cut(s) 8, 21, 124, 617, 1547
BspQI GCTCTTC 1 cut(s) 1134
BspTI CTTAAG 1 cut(s) 1918
BspTNI GGTCTC 1 cut(s) 605
BsrDI GCAATG 2 cut(s) 1641, 1775
BsrGI TGTACA 1 cut(s) 1651
BsrI ACTGG 2 cut(s) 102, 117
BssECI CCNNGG 4 cut(s) 552, 981, 1088, 1212
BssMI GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 1894
BssSI CACGAG 3 cut(s) 81, 365, 1784
BssT1I CCWWGG 2 cut(s) 981, 1212
Bst2BI CACGAG 3 cut(s) 81, 365, 1784
Bst2UI CCWGG 4 cut(s) 554, 1089, 1154, 1658
Bst4CI ACNGT 6 cut(s) 10, 421, 835, 1378, 1793, 1810
Bst6I CTCTTC 2 cut(s) 511, 1134
BstAFI CTTAAG 1 cut(s) 1918
BstAPI GCANNNNNTGC 1 cut(s) 961
BstAUI TGTACA 1 cut(s) 1651
BstBAI YACGTR 2 cut(s) 652, 887
BstC8I GCNNGC 4 cut(s) 24, 338, 1804, 1981
BstDEI CTNAG 9 cut(s) 150, 178, 209, 357, 440, 584, 942, 1192, 2078
BstEII GGTNACC 1 cut(s) 172
BstF5I GGATG 3 cut(s) 394, 1461, 2107
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 1938
BstHHI GCGC 2 cut(s) 1590, 1937
BstKTI GATC 9 cut(s) 16, 119, 247, 571, 625, 1429, 1456, 1542, 1897
BstMAI GTCTC 5 cut(s) 311, 605, 681, 1245, 1760
BstMBI GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 1894
BstNI CCWGG 4 cut(s) 554, 1089, 1154, 1658
BstNSI RCATGY 1 cut(s) 1742
BstPI GGTNACC 1 cut(s) 172
BstSCI CCNGG 4 cut(s) 552, 1087, 1152, 1656
BstSFI CTRYAG 5 cut(s) 604, 1620, 1743, 1938, 2040
BstSLI GKGCMC 2 cut(s) 886, 1063
BstUI CGCG 1 cut(s) 22
BstV2I GAAGAC 2 cut(s) 100, 1289
BstX2I RGATCY 4 cut(s) 13, 116, 1453, 1539
BstYI RGATCY 4 cut(s) 13, 116, 1453, 1539
Bsu36I CCTNAGG 1 cut(s) 178
BsuRI GGCC 5 cut(s) 198, 491, 551, 1079, 1152
BtsCI GGATG 3 cut(s) 394, 1461, 2107
BtsIMutI CAGTG 4 cut(s) 6, 618, 1806, 1872
Cac8I GCNNGC 4 cut(s) 24, 338, 1804, 1981
CaiI CAGNNNCTG 2 cut(s) 185, 1376
CciI TCATGA 2 cut(s) 1597, 1897
CfoI GCGC 2 cut(s) 1590, 1937
Cfr13I GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
CseI GACGC 1 cut(s) 1109
Csp6I GTAC 7 cut(s) 480, 645, 831, 1652, 1789, 1959, 2013
CviQI GTAC 7 cut(s) 480, 645, 831, 1652, 1789, 1959, 2013
DdeI CTNAG 9 cut(s) 150, 178, 209, 357, 440, 584, 942, 1192, 2078
DpnI GATC 9 cut(s) 15, 118, 246, 570, 624, 1428, 1455, 1541, 1896
DpnII GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 1894
DrdI GACNNNNNNGTC 1 cut(s) 303
DseDI GACNNNNNNGTC 1 cut(s) 303
EaeI YGGCCR 2 cut(s) 196, 1150
Eam1104I CTCTTC 2 cut(s) 511, 1134
EarI CTCTTC 2 cut(s) 511, 1134
Ecl136II GAGCTC 2 cut(s) 932, 1979
Eco130I CCWWGG 2 cut(s) 981, 1212
Eco24I GRGCYC 3 cut(s) 934, 1146, 1981
Eco31I GGTCTC 1 cut(s) 605
Eco47I GGWCC 3 cut(s) 611, 1733, 2036
Eco47III AGCGCT 1 cut(s) 1936
Eco53kI GAGCTC 2 cut(s) 932, 1979
Eco57I CTGAAG 3 cut(s) 581, 1454, 1911
Eco72I CACGTG 1 cut(s) 887
Eco81I CCTNAGG 1 cut(s) 178
Eco88I CYCGRG 1 cut(s) 2031
Eco91I GGTNACC 1 cut(s) 172
EcoICRI GAGCTC 2 cut(s) 932, 1979
EcoO109I RGGNCCY 2 cut(s) 490, 2036
EcoO65I GGTNACC 1 cut(s) 172
EcoRII CCWGG 4 cut(s) 552, 1087, 1152, 1656
EcoT14I CCWWGG 2 cut(s) 981, 1212
EcoT22I ATGCAT 1 cut(s) 1883
EcoT38I GRGCYC 3 cut(s) 934, 1146, 1981
ErhI CCWWGG 2 cut(s) 981, 1212
FaqI GGGAC 2 cut(s) 536, 1194
FblI GTMKAC 2 cut(s) 109, 1505
FokI GGATG 3 cut(s) 401, 1448, 2114
FriOI GRGCYC 3 cut(s) 934, 1146, 1981
FspBI CTAG 4 cut(s) 483, 777, 918, 1467
GlaI GCGC 2 cut(s) 1589, 1936
HaeII RGCGCY 1 cut(s) 1938
HaeIII GGCC 5 cut(s) 198, 491, 551, 1079, 1152
HgaI GACGC 1 cut(s) 1109
HhaI GCGC 2 cut(s) 1590, 1937
Hin6I GCGC 2 cut(s) 1588, 1935
HinP1I GCGC 2 cut(s) 1588, 1935
HincII GTYRAC 3 cut(s) 1124, 1506, 1930
HindII GTYRAC 3 cut(s) 1124, 1506, 1930
HindIII AAGCTT 3 cut(s) 393, 923, 1436
HinfI GANTC 9 cut(s) 503, 759, 862, 1238, 1502, 1709, 1965, 2023, 2074
HphI GGTGA 5 cut(s) 98, 184, 1348, 1397, 2117
Hpy166II GTNNAC 9 cut(s) 110, 861, 884, 1124, 1506, 1654, 1733, 1930, 1961
Hpy188I TCNGA 7 cut(s) 401, 415, 1263, 1290, 1706, 1765, 2079
Hpy8I GTNNAC 9 cut(s) 110, 861, 884, 1124, 1506, 1654, 1733, 1930, 1961
Hpy99I CGWCG 1 cut(s) 1976
HpyAV CCTTC 3 cut(s) 481, 556, 2045
HpyCH4III ACNGT 6 cut(s) 10, 421, 835, 1378, 1793, 1810
HpyCH4IV ACGT 2 cut(s) 651, 886
HpyF3I CTNAG 9 cut(s) 150, 178, 209, 357, 440, 584, 942, 1192, 2078
HpySE526I ACGT 2 cut(s) 651, 886
HspAI GCGC 2 cut(s) 1588, 1935
Kzo9I GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 1894
LguI GCTCTTC 1 cut(s) 1134
LmnI GCTCC 2 cut(s) 182, 1976
LweI GCATC 8 cut(s) 313, 460, 838, 967, 1240, 1591, 1789, 1825
MaeI CTAG 4 cut(s) 483, 777, 918, 1467
MaeII ACGT 2 cut(s) 651, 886
MalI GATC 9 cut(s) 15, 118, 246, 570, 624, 1428, 1455, 1541, 1896
MboI GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 1894
MboII GAAGA 9 cut(s) 105, 345, 498, 563, 887, 1105, 1151, 1289, 1904
MfeI CAATTG 1 cut(s) 1839
MflI RGATCY 4 cut(s) 13, 116, 1453, 1539
MhlI GDGCHC 5 cut(s) 886, 934, 1063, 1146, 1981
MlsI TGGCCA 1 cut(s) 1152
MluCI AATT 7 cut(s) 145, 825, 1278, 1570, 1815, 1839, 2134
MluNI TGGCCA 1 cut(s) 1152
MlyI GAGTC 6 cut(s) 497, 856, 1247, 1511, 1718, 2068
MmeI TCCRAC 3 cut(s) 43, 274, 1268
Mox20I TGGCCA 1 cut(s) 1152
Mph1103I ATGCAT 1 cut(s) 1883
MroXI GAANNNNTTC 1 cut(s) 1644
MscI TGGCCA 1 cut(s) 1152
MslI CAYNNNNRTG 6 cut(s) 846, 856, 910, 1533, 1851, 1857
Msp20I TGGCCA 1 cut(s) 1152
MspA1I CMGCKG 2 cut(s) 290, 1034
MspCI CTTAAG 1 cut(s) 1918
MspR9I CCNGG 4 cut(s) 554, 1089, 1154, 1658
MunI CAATTG 1 cut(s) 1839
Mva1269I GAATGC 1 cut(s) 1883
MvaI CCWGG 4 cut(s) 554, 1089, 1154, 1658
MvnI CGCG 1 cut(s) 22
NdeII GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 1894
NlaIV GGNNCC 2 cut(s) 15, 492
NmuCI GTSAC 8 cut(s) 76, 172, 346, 367, 887, 998, 1201, 1868
NsiI ATGCAT 1 cut(s) 1883
NspI RCATGY 1 cut(s) 1742
OliI CACNNNNGTG 1 cut(s) 856
PaeR7I CTCGAG 1 cut(s) 2031
PagI TCATGA 2 cut(s) 1597, 1897
PciSI GCTCTTC 1 cut(s) 1134
PctI GAATGC 1 cut(s) 1883
PdmI GAANNNNTTC 1 cut(s) 1644
PfeI GAWTC 3 cut(s) 759, 1965, 2023
PleI GAGTC 6 cut(s) 497, 856, 1246, 1510, 1717, 2068
PmaCI CACGTG 1 cut(s) 887
PmlI CACGTG 1 cut(s) 887
PpsI GAGTC 6 cut(s) 497, 856, 1246, 1510, 1717, 2068
Ppu21I YACGTR 2 cut(s) 652, 887
PpuMI RGGWCCY 1 cut(s) 2036
Psp124BI GAGCTC 2 cut(s) 934, 1981
Psp5II RGGWCCY 1 cut(s) 2036
Psp6I CCWGG 4 cut(s) 552, 1087, 1152, 1656
PspCI CACGTG 1 cut(s) 887
PspEI GGTNACC 1 cut(s) 172
PspGI CCWGG 4 cut(s) 552, 1087, 1152, 1656
PspN4I GGNNCC 2 cut(s) 15, 492
PspPI GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
PspPPI RGGWCCY 1 cut(s) 2036
PspXI VCTCGAGB 1 cut(s) 2031
PstI CTGCAG 3 cut(s) 1624, 1747, 1942
PstNI CAGNNNCTG 2 cut(s) 185, 1376
PsuI RGATCY 4 cut(s) 13, 116, 1453, 1539
PvuII CAGCTG 2 cut(s) 290, 1034
RsaI GTAC 7 cut(s) 481, 646, 832, 1653, 1790, 1960, 2014
RsaNI GTAC 7 cut(s) 480, 645, 831, 1652, 1789, 1959, 2013
RseI CAYNNNNRTG 6 cut(s) 846, 856, 910, 1533, 1851, 1857
SacI GAGCTC 2 cut(s) 934, 1981
SalI GTCGAC 1 cut(s) 1504
SapI GCTCTTC 1 cut(s) 1134
Sau3AI GATC 9 cut(s) 13, 116, 244, 568, 622, 1426, 1453, 1539, 1894
Sau96I GGNCC 6 cut(s) 490, 550, 611, 1078, 1733, 2036
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 6 cut(s) 497, 856, 1247, 1511, 1718, 2068
ScrFI CCNGG 4 cut(s) 554, 1089, 1154, 1658
SduI GDGCHC 5 cut(s) 886, 934, 1063, 1146, 1981
SfaNI GCATC 8 cut(s) 313, 460, 838, 967, 1240, 1591, 1789, 1825
SfcI CTRYAG 5 cut(s) 604, 1620, 1743, 1938, 2040
Sfr274I CTCGAG 1 cut(s) 2031
SinI GGWCC 3 cut(s) 611, 1733, 2036
SlaI CTCGAG 1 cut(s) 2031
SmiMI CAYNNNNRTG 6 cut(s) 846, 856, 910, 1533, 1851, 1857
SmlI CTYRAG 2 cut(s) 1918, 2031
SmoI CTYRAG 2 cut(s) 1918, 2031
SpeI ACTAGT 1 cut(s) 776
Sse9I AATT 7 cut(s) 145, 825, 1278, 1570, 1815, 1839, 2134
SsiI CCGC 1 cut(s) 747
SspI AATATT 1 cut(s) 960
SspMI CTAG 4 cut(s) 483, 777, 918, 1467
SstI GAGCTC 2 cut(s) 934, 1981
StyD4I CCNGG 4 cut(s) 552, 1087, 1152, 1656
StyI CCWWGG 2 cut(s) 981, 1212
TaaI ACNGT 6 cut(s) 10, 421, 835, 1378, 1793, 1810
TaiI ACGT 2 cut(s) 654, 889
TaqI TCGA 6 cut(s) 928, 1236, 1243, 1505, 2032, 2118
TasI AATT 7 cut(s) 145, 825, 1278, 1570, 1815, 1839, 2134
TatI WGTACW 5 cut(s) 479, 1651, 1788, 1958, 2012
TauI GCSGC 1 cut(s) 749
TfiI GAWTC 3 cut(s) 759, 1965, 2023
TscAI CASTG 4 cut(s) 13, 625, 1813, 1879
TseFI GTSAC 8 cut(s) 76, 172, 346, 367, 887, 998, 1201, 1868
Tsp45I GTSAC 8 cut(s) 76, 172, 346, 367, 887, 998, 1201, 1868
TspDTI ATGAA 8 cut(s) 405, 830, 1008, 1032, 1260, 1586, 1841, 1914
TspGWI ACGGA 2 cut(s) 632, 1989
TspRI CASTG 4 cut(s) 13, 625, 1813, 1879
Vha464I CTTAAG 1 cut(s) 1918
VneI GTGCAC 1 cut(s) 882
VpaK11BI GGWCC 3 cut(s) 611, 1733, 2036
XapI RAATTY 2 cut(s) 145, 2134
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 1 cut(s) 1742
XhoI CTCGAG 1 cut(s) 2031
XmiI GTMKAC 2 cut(s) 109, 1505
XmnI GAANNNNTTC 1 cut(s) 1644
XspI CTAG 4 cut(s) 483, 777, 918, 1467
ZrmI AGTACT 1 cut(s) 481
Zsp2I ATGCAT 1 cut(s) 1883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.