pycom12g14450

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000993
Physical Location & Seq
Reverse (-)
16977056 .. 16978530
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g14450.4

Sequence Viewer

Length: 576 bp
ATGGCGAAGCGAATTGTTCTGGATGCAAAAGTAGACTACCCTGCTGCCTGCAATGCGATGGAAACACTTCTTGTACACAAGGATCTGTTGAGTGACGATGGCGGGTTTAAGCAGATAATTTCAGAACTCAAAAATTCAGGTGTTAGCTTATATGGTGGACCTAGGGCAGCAACTGCGTTACACCTTCCAGAGACAGACTCATTCCATTACGAGTACAGTTCATTGGCTTGCTCAGTTGAAATCGTTGATGATGTGAACGCTGCAATTGACCACATTCATGAACATGGAAGTTCTCATACTGATTGCATCGTCACTGAAGATGGTCAAGTTGCTAAAATTTTCCTAACTCAAGTCGACAGCGCTGCAGTCTTTCATAACGCGAGTACAAGGTTTTGTGATGGAGCACGCTTTGGACTCGGTGCAGAAGTCGGGATAAGTACGAGCAGGATTCATGCTCGAGGTCCTGTAGGAGTTGAAGGATTGCTAACAACAAGATGGATTCTGAGAGGGAACGGGCAGATTGTAGACGCGGATAGAGGAGTCGAGTACACCCACAAGGACCTGCTTAGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

192

Amino Acids

20.55

Weight (kDa)

5.35

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 570
AccI GTMKAC 3 cut(s) 33, 354, 525
AccII CGCG 2 cut(s) 380, 530
AciI CCGC 2 cut(s) 102, 530
AclWI GGATC 1 cut(s) 90
AcsI RAATTY 2 cut(s) 133, 336
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 5 cut(s) 75, 215, 385, 439, 548
AfeI AGCGCT 1 cut(s) 361
AgsI TTSAA 2 cut(s) 239, 476
AluBI AGCT 1 cut(s) 147
AluI AGCT 1 cut(s) 147
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 1 cut(s) 185
AlwI GGATC 1 cut(s) 90
AlwNI CAGNNNCTG 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 456
Aor51HI AGCGCT 1 cut(s) 361
ApeKI GCWGC 4 cut(s) 44, 167, 260, 362
ApoI RAATTY 2 cut(s) 133, 336
Asp700I GAANNNNTTC 1 cut(s) 66
AspA2I CCTAGG 1 cut(s) 161
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 3 cut(s) 158, 461, 559
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 3 cut(s) 158, 461, 559
AvrII CCTAGG 1 cut(s) 161
BarI GAAGNNNNNNTAC 2 cut(s) 280, 312
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 4 cut(s) 31, 179, 247, 349
BccI CCATC 5 cut(s) 52, 92, 314, 392, 489
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 2 cut(s) 363, 465
BfoI RGCGCY 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 570
BisI GCNGC 4 cut(s) 45, 168, 261, 363
BlnI CCTAGG 1 cut(s) 161
BlsI GCNGC 4 cut(s) 46, 169, 262, 364
Bme18I GGWCC 3 cut(s) 158, 461, 559
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 3 cut(s) 158, 461, 559
BmsI GCATC 2 cut(s) 13, 315
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpuEI CTTGAG 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 161
Bse3DI GCAATG 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 1 cut(s) 28
BseMI GCAATG 1 cut(s) 58
BseMII CTCAG 2 cut(s) 246, 494
BseRI GAGGAG 1 cut(s) 552
BseXI GCAGC 4 cut(s) 31, 179, 247, 349
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 2 cut(s) 380, 530
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 456
BsmAI GTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 406
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 2 cut(s) 102, 530
BspCNI CTCAG 2 cut(s) 245, 495
BspFNI CGCG 2 cut(s) 380, 530
BspHI TCATGA 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 570
BspPI GGATC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 58
BsrGI TGTACA 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 173
BstAUI TGTACA 1 cut(s) 73
BstC8I GCNNGC 3 cut(s) 49, 229, 406
BstDEI CTNAG 3 cut(s) 232, 503, 566
BstF5I GGATG 1 cut(s) 28
BstFNI CGCG 2 cut(s) 380, 530
BstH2I RGCGCY 1 cut(s) 363
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 2 cut(s) 53, 173
BstSFI CTRYAG 2 cut(s) 363, 465
BstUI CGCG 2 cut(s) 380, 530
BstV1I GCAGC 4 cut(s) 31, 179, 247, 349
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 1 cut(s) 28
BtsIMutI CAGTG 1 cut(s) 312
BveI ACCTGC 1 cut(s) 570
Cac8I GCNNGC 3 cut(s) 49, 229, 406
CaiI CAGNNNCTG 1 cut(s) 173
CciI TCATGA 1 cut(s) 277
CfoI GCGC 1 cut(s) 362
Cfr13I GGNCC 3 cut(s) 158, 461, 559
CseI GACGC 1 cut(s) 536
Csp6I GTAC 5 cut(s) 74, 214, 384, 438, 547
CviAII CATG 3 cut(s) 278, 284, 452
CviJI RGCY 2 cut(s) 147, 227
CviKI_1 RGCY 2 cut(s) 147, 227
CviQI GTAC 5 cut(s) 74, 214, 384, 438, 547
DdeI CTNAG 3 cut(s) 232, 503, 566
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 161
Eco47I GGWCC 3 cut(s) 158, 461, 559
Eco47III AGCGCT 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 456
EcoO109I RGGNCCY 2 cut(s) 461, 559
EcoT14I CCWWGG 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 3 cut(s) 281, 287, 455
FaiI YATR 7 cut(s) 151, 153, 279, 285, 297, 375, 453
FatI CATG 3 cut(s) 277, 283, 451
FauI CCCGC 1 cut(s) 95
FblI GTMKAC 3 cut(s) 33, 354, 525
Fnu4HI GCNGC 4 cut(s) 45, 168, 261, 363
FokI GGATG 1 cut(s) 35
Fsp4HI GCNGC 4 cut(s) 45, 168, 261, 363
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 361
GluI GCNGC 4 cut(s) 45, 168, 261, 363
HaeII RGCGCY 1 cut(s) 363
HgaI GACGC 1 cut(s) 536
HhaI GCGC 1 cut(s) 362
Hin1II CATG 3 cut(s) 281, 287, 455
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 5 cut(s) 197, 414, 448, 499, 540
Hpy166II GTNNAC 7 cut(s) 34, 76, 158, 256, 355, 526, 549
Hpy188I TCNGA 2 cut(s) 124, 504
Hpy188III TCNNGA 4 cut(s) 20, 188, 278, 430
Hpy8I GTNNAC 7 cut(s) 34, 76, 158, 256, 355, 526, 549
HpyAV CCTTC 2 cut(s) 194, 470
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4V TGCA 6 cut(s) 26, 51, 263, 306, 365, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 173
HpyF3I CTNAG 3 cut(s) 232, 503, 566
Hsp92II CATG 3 cut(s) 281, 287, 455
HspAI GCGC 1 cut(s) 360
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 1 cut(s) 401
LpnPI CCDG 7 cut(s) 5, 54, 61, 123, 201, 430, 477
Lsp1109I GCAGC 4 cut(s) 31, 179, 247, 349
LweI GCATC 2 cut(s) 13, 315
MaeI CTAG 1 cut(s) 162
MaeIII GTNAC 3 cut(s) 92, 177, 310
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 329
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 406
MluCI AATT 5 cut(s) 12, 117, 133, 264, 336
MlyI GAGTC 3 cut(s) 191, 408, 549
MnlI CCTC 3 cut(s) 452, 500, 530
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 2 cut(s) 276, 282
MunI CAATTG 1 cut(s) 264
MvnI CGCG 2 cut(s) 380, 530
MwoI GCNNNNNNNGC 2 cut(s) 53, 173
NdeII GATC 1 cut(s) 82
NlaIII CATG 3 cut(s) 281, 287, 455
NmuCI GTSAC 2 cut(s) 92, 310
PaeR7I CTCGAG 1 cut(s) 456
PagI TCATGA 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 448, 499
PkrI GCNGC 4 cut(s) 46, 169, 262, 364
PleI GAGTC 3 cut(s) 191, 408, 548
PpsI GAGTC 3 cut(s) 191, 408, 548
PpuMI RGGWCCY 2 cut(s) 461, 559
Psp5II RGGWCCY 2 cut(s) 461, 559
PspPI GGNCC 3 cut(s) 158, 461, 559
PspPPI RGGWCCY 2 cut(s) 461, 559
PspXI VCTCGAGB 1 cut(s) 456
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 82
RsaI GTAC 5 cut(s) 75, 215, 385, 439, 548
RsaNI GTAC 5 cut(s) 74, 214, 384, 438, 547
RseI CAYNNNNRTG 2 cut(s) 276, 282
SalI GTCGAC 1 cut(s) 353
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 4 cut(s) 45, 168, 261, 363
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 3 cut(s) 158, 461, 559
SchI GAGTC 3 cut(s) 191, 408, 549
SduI GDGCHC 1 cut(s) 406
SetI ASST 7 cut(s) 142, 149, 163, 186, 392, 463, 564
SfaNI GCATC 2 cut(s) 13, 315
SfcI CTRYAG 2 cut(s) 363, 465
Sfr274I CTCGAG 1 cut(s) 456
SinI GGWCC 3 cut(s) 158, 461, 559
SlaI CTCGAG 1 cut(s) 456
SmiMI CAYNNNNRTG 2 cut(s) 276, 282
SmlI CTYRAG 2 cut(s) 348, 456
SmoI CTYRAG 2 cut(s) 348, 456
Sse9I AATT 5 cut(s) 12, 117, 133, 264, 336
SsiI CCGC 2 cut(s) 102, 530
SspMI CTAG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 1 cut(s) 218
TaqI TCGA 3 cut(s) 354, 457, 543
TasI AATT 5 cut(s) 12, 117, 133, 264, 336
TatI WGTACW 4 cut(s) 73, 213, 383, 546
TfiI GAWTC 2 cut(s) 448, 499
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 2 cut(s) 92, 310
TseI GCWGC 4 cut(s) 44, 167, 260, 362
Tsp45I GTSAC 2 cut(s) 92, 310
TspDTI ATGAA 5 cut(s) 210, 266, 294, 362, 440
TspRI CASTG 1 cut(s) 319
VpaK11BI GGWCC 3 cut(s) 158, 461, 559
XapI RAATTY 2 cut(s) 133, 336
XhoI CTCGAG 1 cut(s) 456
XmaJI CCTAGG 1 cut(s) 161
XmiI GTMKAC 3 cut(s) 33, 354, 525
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.