Rorug03G0075400

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000993
Physical Location & Seq
Forward (+)
5895434 .. 5898530
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0075400.1

Sequence Viewer

Length: 1545 bp
ATGTCATCTTTCCTGTTCAGAAAAAGAGCAAGTAGTAGGAGATGCTGGGGGGTTCTCTGCCGAAACCCTAAAGCTGAATCTCAATTTGGTTATCTCAATAAACCCCCAAAACCTCAGAATGTCACAAGCTCAGTTGAAATTCCGGGGGTTTTGGATCCTGGGTCTTCTTCATTTTCACCCAAAACCTCTAAAACCCTATCTGGGTCTCTCCAGAACATCAAAAACTCAATTGCTTCCGGTGAAAGTTTTGGGCTTTCTTCTAATTTGGGGGTTCCAGTTGTGAGAAGCTTACGTGGGTTTCATGGTGGAATTGCAACTTGTAGCTTGGTTCGATTTTATGGGACTCAGGCTGCTCAGGAGCCTTCGACCGCTGATGGGCTCACAGTTGATCGAATCCTTAATAGTGAGTGGTCAATTATGGCAGAGGATGAGAGTGATTGGAAGAGTCACGCTGCTGCAATTGCCCAATCAGTTCATCTTATTAAGAAGCGTTTGCAGTGGAAGAAGTTGCTGGTGAGGTTGGATATGTTATCAGTGGAACTGAACAAGCCAGACCTGTGGAATGATCCTGTACATGCAGGAAAGATAAGTCGCGAGCATGGTTCATTGATGGGGAAAATGAAAGAAGTGAAGGCATTTGAGCAGGAATTGCTTGAGCATGTGGACATGATAAAGCTTGCTCGGGAGGAGAATGACGTGGAGTTGGAATCGGAATCCATGAAGGCTTTACTTGAGATGAGAAGAACTTCAAAGGAGAAAGAGACCCAAGCTTTGTTAGCTGGGGAGCAGGATCCTTGCTCATGTTATATAGAGGTGCAATCGGGAGCTGGGGGTACAGAGAGCAATGACTGGGCAGCGATGGTCATGCAGATGTATAAAATGTGGGCTCAACGCCGTGGATATAAAATGACCGTGGTGGATGAAATGCCTGGTGAGATTGCGGGAATCAAGCGGGCAACTATCAAAGTTGATGGTGAATATGCTTTTGGATATGCCAAAGCAGAAGTAGGAGTGCACAGGTTGGTACGTATCTCACCTTTTGACAGTAATAAGCGCAGACATACTTCATTTGCTGCTGTCGCTGTGACTCCAATCCTGGGAGATGTATCTGCTCATGTACAGATTAATGAATCTGATCTGCGTATCGAGCGTTTTCGTTCTGGGGGATCTGGAGGCCAGCACGCTAATACGACTGATAGTGCTGTGAGGATTGTTCACATTCCCACTGGAATAACTGCCACTTGTCAAAATGAAAGATCACAACATCAGAATAAGGCTTCAGCAATGGCTGTTATCCAAGCAAGAGTGGACCAGCTTGAAATGGCACGCCAGACTCAGAAGAATGCACAATATTCACAATCTCTTACTGATATAACCTGGGGCAGCCAGATACGCAGCTACGTGCTCCAGCCATACCGCATGGTGAAAGATCTTAGAACCAATTATGAAGTCTCTGATCCTGATTCTGTCCTTGAGGGAGATCTAGACGGTTTTATCTTGAGTTATTTATCAGCTTCCTTGGATAAAGATGAAGATGACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

514

Amino Acids

57.05

Weight (kDa)

7.2

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 173 - 360 6.6e-51 PCRF domain
RF-1 PF00472 372 - 479 6.5e-36 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 594
AciI CCGC 5 cut(s) 369, 941, 952, 1417, 1540
AclWI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
AcsI RAATTY 1 cut(s) 138
AcuI CTGAAG 1 cut(s) 1263
AfaI GTAC 4 cut(s) 573, 835, 1026, 1119
AfiI CCNNNNNNNGG 3 cut(s) 201, 375, 1097
AgsI TTSAA 3 cut(s) 137, 750, 1319
AjiI CACGTC 1 cut(s) 697
AjnI CCWGG 4 cut(s) 157, 928, 1095, 1376
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 969, 1001
Alw21I GWGCWC 2 cut(s) 1017, 1407
Alw26I GTCTC 3 cut(s) 210, 755, 1456
Alw44I GTGCAC 1 cut(s) 1013
AlwI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 1 cut(s) 1174
ApaLI GTGCAC 1 cut(s) 1013
ApeKI GCWGC 7 cut(s) 350, 452, 455, 854, 1073, 1383, 1395
ApoI RAATTY 1 cut(s) 138
ArsI GACNNNNNNTTYG 2 cut(s) 754, 786
AseI ATTAAT 1 cut(s) 1125
Asp700I GAANNNNTTC 1 cut(s) 745
AspLEI GCGC 1 cut(s) 1056
AspS9I GGNCC 1 cut(s) 1309
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 7 cut(s) 168, 251, 526, 944, 986, 1026, 1435
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 1 cut(s) 1309
BaeGI GKGCMC 1 cut(s) 1017
BamHI GGATCC 2 cut(s) 154, 790
BanII GRGCYC 2 cut(s) 381, 889
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 1017, 1407
BbvI GCAGC 7 cut(s) 337, 439, 442, 866, 1060, 1395, 1407
BccI CCATC 4 cut(s) 368, 604, 853, 965
BceAI ACGGC 1 cut(s) 879
BcgI CGANNNNNNTGC 2 cut(s) 847, 881
BciT130I CCWGG 4 cut(s) 159, 930, 1097, 1378
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 3 cut(s) 210, 755, 1456
BfaI CTAG 1 cut(s) 1484
BglII AGATCT 2 cut(s) 1429, 1480
BisI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
BlsI GCNGC 7 cut(s) 352, 454, 457, 856, 1075, 1385, 1397
Bme1390I CCNGG 5 cut(s) 144, 159, 930, 1097, 1378
Bme18I GGWCC 1 cut(s) 1309
BmeT110I CYCGRG 1 cut(s) 681
BmgBI CACGTC 1 cut(s) 697
BmgT120I GGNCC 1 cut(s) 1309
BmiI GGNNCC 4 cut(s) 156, 273, 360, 792
BmrFI CCNGG 5 cut(s) 144, 159, 930, 1097, 1378
BmrI ACTGGG 1 cut(s) 859
BmsI GCATC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 859
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 3 cut(s) 194, 1191, 1391
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 4 cut(s) 674, 752, 1493, 1519
BpuMI CCSGG 1 cut(s) 144
BsaAI YACGTR 3 cut(s) 293, 1028, 1402
BsaI GGTCTC 2 cut(s) 210, 755
BsaJI CCNNGG 7 cut(s) 143, 158, 895, 912, 1096, 1377, 1518
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 375, 1097
Bse1I ACTGG 3 cut(s) 275, 854, 1231
Bse3DI GCAATG 2 cut(s) 850, 1290
BseBI CCWGG 4 cut(s) 159, 930, 1097, 1378
BseDI CCNNGG 7 cut(s) 143, 158, 895, 912, 1096, 1377, 1518
BseGI GGATG 2 cut(s) 433, 925
BseLI CCNNNNNNNGG 3 cut(s) 201, 375, 1097
BseMI GCAATG 2 cut(s) 850, 1290
BseMII CTCAG 5 cut(s) 128, 144, 359, 368, 1349
BseNI ACTGG 3 cut(s) 275, 854, 1231
BseRI GAGGAG 1 cut(s) 701
BseSI GKGCMC 1 cut(s) 1017
BseXI GCAGC 7 cut(s) 337, 439, 442, 866, 1060, 1395, 1407
BseYI CCCAGC 3 cut(s) 45, 779, 827
Bsh1236I CGCG 1 cut(s) 594
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 1176
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 2 cut(s) 1017, 1407
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 2 cut(s) 143, 237
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 3 cut(s) 201, 375, 1097
BsmAI GTCTC 3 cut(s) 210, 755, 1456
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 1 cut(s) 1176
Bso31I GGTCTC 2 cut(s) 210, 755
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 4 cut(s) 381, 889, 1017, 1407
Bsp1407I TGTACA 2 cut(s) 571, 1117
Bsp68I TCGCGA 1 cut(s) 594
BspACI CCGC 5 cut(s) 369, 941, 952, 1417, 1540
BspANI GGCC 1 cut(s) 1176
BspCNI CTCAG 5 cut(s) 127, 143, 358, 367, 1348
BspFNI CGCG 1 cut(s) 594
BspLI GGNNCC 4 cut(s) 156, 273, 360, 792
BspPI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
BspTNI GGTCTC 2 cut(s) 210, 755
BsrDI GCAATG 2 cut(s) 850, 1290
BsrGI TGTACA 2 cut(s) 571, 1117
BsrI ACTGG 3 cut(s) 275, 854, 1231
BssECI CCNNGG 7 cut(s) 143, 158, 895, 912, 1096, 1377, 1518
BssT1I CCWWGG 1 cut(s) 1518
Bst2UI CCWGG 4 cut(s) 159, 930, 1097, 1378
Bst4CI ACNGT 4 cut(s) 385, 913, 1046, 1490
Bst6I CTCTTC 1 cut(s) 437
BstAPI GCANNNNNTGC 1 cut(s) 649
BstAUI TGTACA 2 cut(s) 571, 1117
BstBAI YACGTR 3 cut(s) 293, 1028, 1402
BstC8I GCNNGC 6 cut(s) 596, 678, 954, 1178, 1182, 1327
BstDEI CTNAG 6 cut(s) 114, 130, 345, 354, 1335, 1433
BstDSI CCRYGG 2 cut(s) 895, 912
BstF5I GGATG 2 cut(s) 433, 925
BstFNI CGCG 1 cut(s) 594
BstHHI GCGC 1 cut(s) 1056
BstMAI GTCTC 3 cut(s) 210, 755, 1456
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 6 cut(s) 461, 649, 776, 1079, 1147, 1392
BstNI CCWGG 4 cut(s) 159, 930, 1097, 1378
BstNSI RCATGY 2 cut(s) 578, 662
BstSCI CCNGG 5 cut(s) 142, 157, 928, 1095, 1376
BstSLI GKGCMC 1 cut(s) 1017
BstSNI TACGTA 1 cut(s) 1028
BstUI CGCG 1 cut(s) 594
BstV1I GCAGC 7 cut(s) 337, 439, 442, 866, 1060, 1395, 1407
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
BstXI CCANNNNNNTGG 1 cut(s) 558
BstYI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
BsuRI GGCC 1 cut(s) 1176
BtgI CCRYGG 2 cut(s) 895, 912
BtgZI GCGATG 1 cut(s) 872
BtrI CACGTC 1 cut(s) 697
BtsCI GGATG 2 cut(s) 433, 925
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 3 cut(s) 503, 540, 1224
BtuMI TCGCGA 1 cut(s) 594
Cac8I GCNNGC 6 cut(s) 596, 678, 954, 1178, 1182, 1327
CfoI GCGC 1 cut(s) 1056
Cfr13I GGNCC 1 cut(s) 1309
Csp6I GTAC 4 cut(s) 572, 834, 1025, 1118
CviQI GTAC 4 cut(s) 572, 834, 1025, 1118
DdeI CTNAG 6 cut(s) 114, 130, 345, 354, 1335, 1433
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Eco105I TACGTA 1 cut(s) 1028
Eco130I CCWWGG 1 cut(s) 1518
Eco24I GRGCYC 2 cut(s) 381, 889
Eco31I GGTCTC 2 cut(s) 210, 755
Eco47I GGWCC 1 cut(s) 1309
Eco57I CTGAAG 1 cut(s) 1263
Eco88I CYCGRG 1 cut(s) 681
EcoRII CCWGG 4 cut(s) 157, 928, 1095, 1376
EcoT14I CCWWGG 1 cut(s) 1518
EcoT38I GRGCYC 2 cut(s) 381, 889
ErhI CCWWGG 1 cut(s) 1518
FaqI GGGAC 1 cut(s) 355
FauI CCCGC 2 cut(s) 934, 945
Fnu4HI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
FokI GGATG 2 cut(s) 440, 932
FriOI GRGCYC 2 cut(s) 381, 889
Fsp4HI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
FspBI CTAG 1 cut(s) 1484
GlaI GCGC 1 cut(s) 1055
GluI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
GsaI CCCAGC 3 cut(s) 49, 783, 831
GsuI CTGGAG 3 cut(s) 194, 1191, 1391
HaeIII GGCC 1 cut(s) 1176
HapII CCGG 2 cut(s) 143, 237
HhaI GCGC 1 cut(s) 1056
Hin6I GCGC 1 cut(s) 1054
HinP1I GCGC 1 cut(s) 1054
HindIII AAGCTT 3 cut(s) 286, 674, 768
HpaII CCGG 2 cut(s) 143, 237
HphI GGTGA 7 cut(s) 168, 251, 526, 944, 986, 1026, 1435
Hpy166II GTNNAC 4 cut(s) 664, 1015, 1216, 1309
Hpy188I TCNGA 7 cut(s) 20, 117, 712, 1135, 1269, 1338, 1456
Hpy188III TCNNGA 9 cut(s) 211, 356, 593, 683, 822, 1170, 1460, 1484, 1498
Hpy8I GTNNAC 4 cut(s) 664, 1015, 1216, 1309
HpyAV CCTTC 3 cut(s) 372, 625, 715
HpyCH4III ACNGT 4 cut(s) 385, 913, 1046, 1490
HpyCH4IV ACGT 4 cut(s) 292, 696, 1027, 1401
HpyCH4V TGCA 8 cut(s) 314, 458, 496, 578, 817, 868, 1015, 1346
HpyF10VI GCNNNNNNNGC 6 cut(s) 461, 649, 776, 1079, 1147, 1392
HpyF3I CTNAG 6 cut(s) 114, 130, 345, 354, 1335, 1433
HpySE526I ACGT 4 cut(s) 292, 696, 1027, 1401
HspAI GCGC 1 cut(s) 1054
LmnI GCTCC 4 cut(s) 358, 784, 824, 1410
Lsp1109I GCAGC 7 cut(s) 337, 439, 442, 866, 1060, 1395, 1407
LweI GCATC 1 cut(s) 32
MaeI CTAG 1 cut(s) 1484
MaeII ACGT 4 cut(s) 292, 696, 1027, 1401
MaeIII GTNAC 3 cut(s) 121, 446, 1084
MboII GAAGA 8 cut(s) 156, 159, 249, 454, 514, 753, 1351, 1544
MfeI CAATTG 2 cut(s) 228, 459
MflI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
MhlI GDGCHC 4 cut(s) 381, 889, 1017, 1407
MluCI AATT 9 cut(s) 83, 138, 228, 262, 309, 414, 459, 647, 1441
MlyI GAGTC 4 cut(s) 337, 454, 1081, 1327
MmeI TCCRAC 2 cut(s) 501, 684
MnlI CCTC 9 cut(s) 123, 196, 418, 510, 679, 805, 1166, 1200, 1468
MroXI GAANNNNTTC 1 cut(s) 745
MseI TTAA 3 cut(s) 399, 483, 1125
MslI CAYNNNNRTG 1 cut(s) 869
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 2 cut(s) 143, 237
MspR9I CCNGG 5 cut(s) 144, 159, 930, 1097, 1378
MunI CAATTG 2 cut(s) 228, 459
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 4 cut(s) 159, 930, 1097, 1378
MvnI CGCG 1 cut(s) 594
MwoI GCNNNNNNNGC 6 cut(s) 461, 649, 776, 1079, 1147, 1392
NciI CCSGG 1 cut(s) 144
NlaIV GGNNCC 4 cut(s) 156, 273, 360, 792
NmuCI GTSAC 3 cut(s) 121, 446, 1084
NruI TCGCGA 1 cut(s) 594
NspI RCATGY 2 cut(s) 578, 662
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 745
PfeI GAWTC 7 cut(s) 77, 393, 707, 713, 945, 1130, 1463
PkrI GCNGC 7 cut(s) 352, 454, 457, 856, 1075, 1385, 1397
PleI GAGTC 4 cut(s) 337, 453, 1081, 1327
PpsI GAGTC 4 cut(s) 337, 453, 1081, 1327
Ppu21I YACGTR 3 cut(s) 293, 1028, 1402
PshBI ATTAAT 1 cut(s) 1125
Psp6I CCWGG 4 cut(s) 157, 928, 1095, 1376
PspFI CCCAGC 3 cut(s) 45, 779, 827
PspGI CCWGG 4 cut(s) 157, 928, 1095, 1376
PspN4I GGNNCC 4 cut(s) 156, 273, 360, 792
PspPI GGNCC 1 cut(s) 1309
PsuI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
RruI TCGCGA 1 cut(s) 594
RsaI GTAC 4 cut(s) 573, 835, 1026, 1119
RsaNI GTAC 4 cut(s) 572, 834, 1025, 1118
RseI CAYNNNNRTG 1 cut(s) 869
SaqAI TTAA 3 cut(s) 399, 483, 1125
SatI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
Sau96I GGNCC 1 cut(s) 1309
SchI GAGTC 4 cut(s) 337, 454, 1081, 1327
ScrFI CCNGG 5 cut(s) 144, 159, 930, 1097, 1378
SduI GDGCHC 4 cut(s) 381, 889, 1017, 1407
SfaNI GCATC 1 cut(s) 32
SinI GGWCC 1 cut(s) 1309
SmiMI CAYNNNNRTG 1 cut(s) 869
SmlI CTYRAG 4 cut(s) 653, 731, 1472, 1498
SmoI CTYRAG 4 cut(s) 653, 731, 1472, 1498
SnaBI TACGTA 1 cut(s) 1028
Sse9I AATT 9 cut(s) 83, 138, 228, 262, 309, 414, 459, 647, 1441
SsiI CCGC 5 cut(s) 369, 941, 952, 1417, 1540
SspI AATATT 1 cut(s) 1352
SspMI CTAG 1 cut(s) 1484
StyD4I CCNGG 5 cut(s) 142, 157, 928, 1095, 1376
StyI CCWWGG 1 cut(s) 1518
TaaI ACNGT 4 cut(s) 385, 913, 1046, 1490
TaiI ACGT 4 cut(s) 295, 699, 1030, 1404
TaqI TCGA 4 cut(s) 331, 365, 391, 1146
TasI AATT 9 cut(s) 83, 138, 228, 262, 309, 414, 459, 647, 1441
TatI WGTACW 2 cut(s) 571, 1117
TfiI GAWTC 7 cut(s) 77, 393, 707, 713, 945, 1130, 1463
Tru1I TTAA 3 cut(s) 399, 483, 1125
Tru9I TTAA 3 cut(s) 399, 483, 1125
TscAI CASTG 3 cut(s) 503, 540, 1231
TseFI GTSAC 3 cut(s) 121, 446, 1084
TseI GCWGC 7 cut(s) 350, 452, 455, 854, 1073, 1383, 1395
Tsp45I GTSAC 3 cut(s) 121, 446, 1084
TspRI CASTG 3 cut(s) 503, 540, 1231
VneI GTGCAC 1 cut(s) 1013
VpaK11BI GGWCC 1 cut(s) 1309
VspI ATTAAT 1 cut(s) 1125
XapI RAATTY 1 cut(s) 138
XbaI TCTAGA 1 cut(s) 1483
XceI RCATGY 2 cut(s) 578, 662
XmnI GAANNNNTTC 1 cut(s) 745
XspI CTAG 1 cut(s) 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.