Prupe.6G262300_v2.0.a1

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000993
Physical Location & Seq
Reverse (-)
25237207 .. 25244161
6955 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G262300.2

Sequence Viewer

Length: 2169 bp
ATGGACTCTGTGGATCCTACCAGAGCTTTTGTCAATCGTGTCAAGCGTATCGTCGTTAAGGTGGGAACAGCTGTGGTCACTCGTGGTGATGGAAGATTAGCTTTGGGCAGACTAGGTGCTCTTTGTGAGCAGCTCAAAGATATAAACGCTCAGGGGTATGAGGTTATTCTGGTGACTTCAGGAGCTGTTGGTCTTGGCCGTCAAAGGCTAAGATACAGGAGATTGGCAAACAGCAGCTTCGCTGATCTCCAAAATCCACAAAATGACTTCGATGGAAAGGCTTGTGCAGCTGTCGGACAGAGCAGTCTCATGGCTCTTTATGATACCATGTTCAGCCAGCTTGATGTGACTTCTTCTCAACTTCTGGTGACGGATAGTGTTTTTCGGGATGAAGCTTTCAGAAAGCAACTTTCTGAAACTGTGAAGTCATTGTTGAAGCTGAGGGTTGTTCCTATATTCAATGAAAATGATGCTGTTAGTACTAGGAAAGCTCCTTATGAGTTCTGTTTGCAGGATTCTTCTGGTATATTTTGGGACAATGACAGTTTGGCGGGTCTATTGGCTCTTGAACTAAAAGCTGACCTTCTTGTTTTACTAAGCGATGTAGAGGGCCTCTATGATGGTCCCCCTACTGACCCAAAATCAAAGTTAATCCATACATACGTCAAAGAAATACATCATGGAGAAATAACATTTGGAGACAAGTCAAGGGTGGGCAGAGGGGGTATGACAGCTAAAGTCAATGCTGCTGTTTGTGCAGCTTATGGTGGAATCCCTGTTGTTATCACTAGTGGCTATGCCACAGACAACATAAAAAAAGTGCTTCGTGGAGACCGGATAGGTACTGTCTTTCATCAAGATGCACATTTGTGGACTCTTGTTAAAGAAGAAGGTGCACGTGATTTGGCTGTTTCGGCACGGGAATCTTCTAGACAGCTTCGAGCTCTAGGATCTAAAGATAGGAGGAAAATTTTGCTTGACGTAGCTGATGCCTTGGTGGCAAATGAAGGTCAGATTATAGCTGAGAATGAAGCTGATATTGCTGTTGCACAGGAGAATGGATATGAAAAGGCCTTAATATCTCGTTTGGCCCTAAAGCCTGGGAAGATCGCTGCTCTTGCAACGTCTATACGTAAGCTTGCAGACATGGAAGAGCCCATTGGCCGTGTTCTACAGAAAACAGAGCTTGCAGAAGGACTTGTCTTAGAGAAAGTGTCTTGTCCTTTGGGTGTTCTTTTAGTTGTTTTTGAGTCTCGACCTGATGCCCTCGTTCAGATAGCTTCATTAGCAATTCAAAGTCGGAATGGTCTTCTGCTTAAAGGAGGGAAAGAGGCCAAGCGGTCGAATGCAATCTTGCACAAGGTCATCACCTCAGCAATCCCAGAAAATGTTGGTGGCAAACTTATTGGACTTGTGAATACAAGAGACGAGATTCCCGATCTGCTCAAGCTGGATGATGTGATAGATCTTGTCATTCCTAGAGGCAGCAATAAGCTCGTTTCTCAAATTAAGGAGTCCACTAAGATACCAGTTCTTGGTCATGCCGATGGAATTTGTCATGTTTATATTGACAAGTCAGCTAATAAGGACATGGCAAAGCGCATTGTTTTGGACGCGAAAACGGATTATCCAGCTGCCTGTAATGCGATGGAAACACTTCTTGTACACAAGGACTTGGTGACTGATGATGGTGGGTTTAAGCAGATAATTTCAGGACTCCAAGATTTAGGTGTTACGTTATATGGTGGACCAAAGGCATCAACTGTGTTAAATATTCCAGAGACAAGCTCATTCCATCATGAGTACAGTTCATTGGCTTGCGCAGTTGAAATTGTTGATGATGTGACTGCCGCGATTAACCACATCCATGAACATGGAAGTGCTCATACTGATTGCATTGTTACAGAAGATCATGAAGTTGCTGAAACTTTCCTAAGTCAAGTGGACAGCGCTGCAATCTTTCATAATGCAAGTACACGATTTTGTGATGGTGCACGCTTTGGACTTGGTGCAGAGGTTGGGATAAGTACGAGCAGGATTCATGCTCGAGGTCCTGTAGGAGTTGAAGGATTGTTAACAACAAGATGGATTCTGAGAGGGAATGGGCAGGTTGTAGACAGCGATAGAGGGGTCAAGTACACCCACAAGGACCTGCTTACAAGTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

723

Amino Acids

77.97

Weight (kDa)

6.28

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 303
Acc16I TGCGCA 1 cut(s) 1822
Acc36I ACCTGC 2 cut(s) 2098, 2160
AccB7I CCANNNNNTGG 1 cut(s) 1535
AccI GTMKAC 1 cut(s) 2115
AccII CGCG 2 cut(s) 1616, 1853
AciI CCGC 3 cut(s) 551, 1339, 1851
AclWI GGATC 3 cut(s) 8, 21, 958
AcoI YGGCCR 2 cut(s) 196, 1162
AcsI RAATTY 2 cut(s) 969, 1551
AcuI CTGAAG 1 cut(s) 162
AcvI CACGTG 1 cut(s) 899
AfaI GTAC 8 cut(s) 481, 844, 1665, 1805, 1975, 2029, 2138, 2164
AfeI AGCGCT 1 cut(s) 1951
AfiI CCNNNNNNNGG 1 cut(s) 1535
AgsI TTSAA 6 cut(s) 436, 460, 569, 1295, 1829, 2066
AhlI ACTAGT 1 cut(s) 788
AjnI CCWGG 1 cut(s) 1099
AjuI GAANNNNNNNTTGG 4 cut(s) 678, 710, 1143, 1175
AleI CACNNNNGTG 1 cut(s) 868
Alw21I GWGCWC 5 cut(s) 121, 898, 946, 1885, 1996
Alw26I GTCTC 6 cut(s) 311, 693, 825, 1257, 1419, 1775
Alw44I GTGCAC 2 cut(s) 894, 1992
AlwI GGATC 3 cut(s) 8, 21, 958
AlwNI CAGNNNCTG 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 2046
Aor51HI AGCGCT 1 cut(s) 1951
AoxI GGCC 6 cut(s) 196, 610, 1071, 1089, 1162, 1332
ApaLI GTGCAC 2 cut(s) 894, 1992
ApeKI GCWGC 9 cut(s) 130, 234, 287, 746, 758, 1112, 1485, 1634, 1952
ApoI RAATTY 2 cut(s) 969, 1551
Asp700I GAANNNNTTC 1 cut(s) 1656
AspLEI GCGC 3 cut(s) 1602, 1823, 1952
AspS9I GGNCC 6 cut(s) 610, 623, 1090, 1748, 2051, 2149
AsuHPI GGTGA 5 cut(s) 98, 184, 379, 1360, 1690
AvaI CYCGRG 1 cut(s) 2046
AvaII GGWCC 4 cut(s) 623, 1748, 2051, 2149
BaeGI GKGCMC 2 cut(s) 898, 1996
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 2 cut(s) 946, 1158
BarI GAAGNNNNNNTAC 2 cut(s) 1870, 1902
BauI CACGAG 1 cut(s) 81
BbrPI CACGTG 1 cut(s) 899
BbsI GAAGAC 1 cut(s) 1301
Bbv12I GWGCWC 5 cut(s) 121, 898, 946, 1885, 1996
BbvCI CCTCAGC 2 cut(s) 440, 1372
BbvI GCAGC 9 cut(s) 142, 246, 299, 733, 770, 1099, 1497, 1621, 1939
BccI CCATC 9 cut(s) 83, 266, 614, 1541, 1642, 1682, 1803, 1982, 2079
BceAI ACGGC 2 cut(s) 183, 1149
BcgI CGANNNNNNTGC 2 cut(s) 1477, 1511
BciT130I CCWGG 1 cut(s) 1101
BcoDI GTCTC 6 cut(s) 311, 693, 825, 1257, 1419, 1775
BcuI ACTAGT 1 cut(s) 788
BfaI CTAG 6 cut(s) 113, 483, 789, 930, 947, 1479
BfmI CTRYAG 2 cut(s) 1172, 2055
BfoI RGCGCY 1 cut(s) 1953
BfuAI ACCTGC 2 cut(s) 2098, 2160
BglI GCCNNNNNGGC 1 cut(s) 998
BglII AGATCT 1 cut(s) 1465
BmcAI AGTACT 1 cut(s) 481
Bme1390I CCNGG 1 cut(s) 1101
Bme18I GGWCC 4 cut(s) 623, 1748, 2051, 2149
BmeT110I CYCGRG 1 cut(s) 2046
BmgT120I GGNCC 6 cut(s) 610, 623, 1090, 1748, 2051, 2149
BmiI GGNNCC 2 cut(s) 15, 625
BmrFI CCNGG 1 cut(s) 1101
BmsI GCATC 5 cut(s) 460, 850, 979, 1252, 1766
BpiI GAAGAC 1 cut(s) 1301
BplI GAGNNNNNCTC 2 cut(s) 1772, 1804
Bpu10I CCTNAGC 3 cut(s) 150, 440, 1372
BpuEI CTTGAG 1 cut(s) 1430
BsaAI YACGTR 2 cut(s) 899, 1133
BsaI GGTCTC 1 cut(s) 825
BsaJI CCNNGG 2 cut(s) 993, 1100
BsaWI WCCGGW 1 cut(s) 834
Bsc4I CCNNNNNNNGG 1 cut(s) 1535
Bse1I ACTGG 1 cut(s) 1529
BseBI CCWGG 1 cut(s) 1101
BseDI CCNNGG 2 cut(s) 993, 1100
BseGI GGATG 3 cut(s) 394, 1459, 1863
BseLI CCNNNNNNNGG 1 cut(s) 1535
BseMII CTCAG 5 cut(s) 164, 431, 1014, 1386, 2084
BseNI ACTGG 1 cut(s) 1529
BseSI GKGCMC 2 cut(s) 898, 1996
BseXI GCAGC 9 cut(s) 142, 246, 299, 733, 770, 1099, 1497, 1621, 1939
BsgI GTGCAG 3 cut(s) 306, 777, 2031
Bsh1236I CGCG 2 cut(s) 1616, 1853
Bsh1285I CGRYCG 1 cut(s) 1343
BshFI GGCC 6 cut(s) 198, 612, 1073, 1091, 1164, 1334
BsiEI CGRYCG 1 cut(s) 1343
BsiHKAI GWGCWC 5 cut(s) 121, 898, 946, 1885, 1996
BsiHKCI CYCGRG 1 cut(s) 2046
BsiSI CCGG 1 cut(s) 835
BslFI GGGAC 2 cut(s) 548, 609
BslI CCNNNNNNNGG 1 cut(s) 1535
BsmAI GTCTC 6 cut(s) 311, 693, 825, 1257, 1419, 1775
BsmBI CGTCTC 1 cut(s) 1419
BsmFI GGGAC 2 cut(s) 548, 609
BsmI GAATGC 1 cut(s) 1351
BsnI GGCC 6 cut(s) 198, 612, 1073, 1091, 1164, 1334
Bso31I GGTCTC 1 cut(s) 825
BsoBI CYCGRG 1 cut(s) 2046
Bsp1286I GDGCHC 6 cut(s) 121, 898, 946, 1158, 1885, 1996
Bsp1407I TGTACA 1 cut(s) 1663
Bsp143I GATC 7 cut(s) 13, 244, 950, 1107, 1438, 1465, 1909
BspACI CCGC 3 cut(s) 551, 1339, 1851
BspANI GGCC 6 cut(s) 198, 612, 1073, 1091, 1164, 1334
BspCNI CTCAG 5 cut(s) 163, 432, 1015, 1385, 2085
BspFNI CGCG 2 cut(s) 1616, 1853
BspHI TCATGA 2 cut(s) 1798, 1912
BspLI GGNNCC 2 cut(s) 15, 625
BspMI ACCTGC 2 cut(s) 2098, 2160
BspPI GGATC 3 cut(s) 8, 21, 958
BspQI GCTCTTC 1 cut(s) 1146
BspTNI GGTCTC 1 cut(s) 825
BsrGI TGTACA 1 cut(s) 1663
BsrI ACTGG 1 cut(s) 1529
BssECI CCNNGG 2 cut(s) 993, 1100
BssMI GATC 7 cut(s) 13, 244, 950, 1107, 1438, 1465, 1909
BssSI CACGAG 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 993
Bst2BI CACGAG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 1101
Bst4CI ACNGT 5 cut(s) 421, 545, 847, 1765, 1808
Bst6I CTCTTC 1 cut(s) 1146
BstAUI TGTACA 1 cut(s) 1663
BstBAI YACGTR 2 cut(s) 899, 1133
BstC8I GCNNGC 5 cut(s) 338, 1140, 1188, 1819, 1996
BstF5I GGATG 3 cut(s) 394, 1459, 1863
BstFNI CGCG 2 cut(s) 1616, 1853
BstH2I RGCGCY 1 cut(s) 1953
BstHHI GCGC 3 cut(s) 1602, 1823, 1952
BstKTI GATC 7 cut(s) 16, 247, 953, 1110, 1441, 1468, 1912
BstMAI GTCTC 6 cut(s) 311, 693, 825, 1257, 1419, 1775
BstMBI GATC 7 cut(s) 13, 244, 950, 1107, 1438, 1465, 1909
BstMCI CGRYCG 1 cut(s) 1343
BstMWI GCNNNNNNNGC 8 cut(s) 287, 755, 914, 998, 1040, 1118, 1286, 1643
BstNI CCWGG 1 cut(s) 1101
BstSCI CCNGG 1 cut(s) 1099
BstSFI CTRYAG 2 cut(s) 1172, 2055
BstSLI GKGCMC 2 cut(s) 898, 1996
BstSNI TACGTA 1 cut(s) 1133
BstUI CGCG 2 cut(s) 1616, 1853
BstV1I GCAGC 9 cut(s) 142, 246, 299, 733, 770, 1099, 1497, 1621, 1939
BstV2I GAAGAC 1 cut(s) 1301
BstX2I RGATCY 3 cut(s) 13, 950, 1465
BstXI CCANNNNNNTGG 1 cut(s) 1874
BstYI RGATCY 3 cut(s) 13, 950, 1465
BsuRI GGCC 6 cut(s) 198, 612, 1073, 1091, 1164, 1334
BtgZI GCGATG 2 cut(s) 615, 1661
BtsCI GGATG 3 cut(s) 394, 1459, 1863
BveI ACCTGC 2 cut(s) 2098, 2160
Cac8I GCNNGC 5 cut(s) 338, 1140, 1188, 1819, 1996
CaiI CAGNNNCTG 1 cut(s) 185
CciI TCATGA 2 cut(s) 1798, 1912
CfoI GCGC 3 cut(s) 1602, 1823, 1952
Cfr13I GGNCC 6 cut(s) 610, 623, 1090, 1748, 2051, 2149
CseI GACGC 1 cut(s) 1622
Csp6I GTAC 8 cut(s) 480, 843, 1664, 1804, 1974, 2028, 2137, 2163
CviQI GTAC 8 cut(s) 480, 843, 1664, 1804, 1974, 2028, 2137, 2163
DpnI GATC 7 cut(s) 15, 246, 952, 1109, 1440, 1467, 1911
DpnII GATC 7 cut(s) 13, 244, 950, 1107, 1438, 1465, 1909
DrdI GACNNNNNNGTC 1 cut(s) 303
DseDI GACNNNNNNGTC 1 cut(s) 303
EaeI YGGCCR 2 cut(s) 196, 1162
Eam1104I CTCTTC 1 cut(s) 1146
EarI CTCTTC 1 cut(s) 1146
Ecl136II GAGCTC 1 cut(s) 944
Eco105I TACGTA 1 cut(s) 1133
Eco130I CCWWGG 1 cut(s) 993
Eco147I AGGCCT 1 cut(s) 1073
Eco24I GRGCYC 2 cut(s) 946, 1158
Eco31I GGTCTC 1 cut(s) 825
Eco47I GGWCC 4 cut(s) 623, 1748, 2051, 2149
Eco47III AGCGCT 1 cut(s) 1951
Eco53kI GAGCTC 1 cut(s) 944
Eco57I CTGAAG 1 cut(s) 162
Eco72I CACGTG 1 cut(s) 899
Eco88I CYCGRG 1 cut(s) 2046
EcoICRI GAGCTC 1 cut(s) 944
EcoO109I RGGNCCY 3 cut(s) 610, 2051, 2149
EcoRII CCWGG 1 cut(s) 1099
EcoT14I CCWWGG 1 cut(s) 993
EcoT38I GRGCYC 2 cut(s) 946, 1158
ErhI CCWWGG 1 cut(s) 993
Esp3I CGTCTC 1 cut(s) 1419
FalI AAGNNNNNCTT 4 cut(s) 85, 117, 567, 599
FaqI GGGAC 2 cut(s) 548, 609
FauI CCCGC 1 cut(s) 544
FblI GTMKAC 1 cut(s) 2115
FokI GGATG 3 cut(s) 401, 1466, 1850
FriOI GRGCYC 2 cut(s) 946, 1158
FspBI CTAG 6 cut(s) 113, 483, 789, 930, 947, 1479
FspI TGCGCA 1 cut(s) 1822
GlaI GCGC 3 cut(s) 1601, 1822, 1951
HaeII RGCGCY 1 cut(s) 1953
HaeIII GGCC 6 cut(s) 198, 612, 1073, 1091, 1164, 1334
HapII CCGG 1 cut(s) 835
HgaI GACGC 1 cut(s) 1622
HhaI GCGC 3 cut(s) 1602, 1823, 1952
Hin6I GCGC 3 cut(s) 1600, 1821, 1950
HinP1I GCGC 3 cut(s) 1600, 1821, 1950
HincII GTYRAC 1 cut(s) 2076
HindII GTYRAC 1 cut(s) 2076
HindIII AAGCTT 2 cut(s) 393, 1136
HpaI GTTAAC 1 cut(s) 2076
HpaII CCGG 1 cut(s) 835
HphI GGTGA 5 cut(s) 98, 184, 379, 1360, 1690
Hpy188I TCNGA 7 cut(s) 296, 401, 415, 1014, 1275, 1302, 2094
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 5 cut(s) 593, 884, 1001, 1187, 2060
HpyCH4III ACNGT 5 cut(s) 421, 545, 847, 1765, 1808
HpyCH4IV ACGT 6 cut(s) 663, 898, 981, 1124, 1132, 1736
HpyF10VI GCNNNNNNNGC 8 cut(s) 287, 755, 914, 998, 1040, 1118, 1286, 1643
HpySE526I ACGT 6 cut(s) 663, 898, 981, 1124, 1132, 1736
HspAI GCGC 3 cut(s) 1600, 1821, 1950
KspAI GTTAAC 1 cut(s) 2076
Kzo9I GATC 7 cut(s) 13, 244, 950, 1107, 1438, 1465, 1909
LguI GCTCTTC 1 cut(s) 1146
LmnI GCTCC 2 cut(s) 182, 496
Lsp1109I GCAGC 9 cut(s) 142, 246, 299, 733, 770, 1099, 1497, 1621, 1939
LweI GCATC 5 cut(s) 460, 850, 979, 1252, 1766
MaeI CTAG 6 cut(s) 113, 483, 789, 930, 947, 1479
MaeII ACGT 6 cut(s) 663, 898, 981, 1124, 1132, 1736
MaeIII GTNAC 8 cut(s) 76, 172, 346, 367, 1678, 1732, 1843, 1900
MalI GATC 7 cut(s) 15, 246, 952, 1109, 1440, 1467, 1911
MboI GATC 7 cut(s) 13, 244, 950, 1107, 1438, 1465, 1909
MboII GAAGA 9 cut(s) 105, 345, 510, 899, 918, 1117, 1163, 1301, 1919
MflI RGATCY 3 cut(s) 13, 950, 1465
MhlI GDGCHC 6 cut(s) 121, 898, 946, 1158, 1885, 1996
MluCI AATT 6 cut(s) 969, 1290, 1506, 1551, 1707, 1830
MlyI GAGTC 4 cut(s) 868, 1259, 1523, 1710
MmeI TCCRAC 2 cut(s) 274, 1280
MroXI GAANNNNTTC 1 cut(s) 1656
MslI CAYNNNNRTG 6 cut(s) 858, 868, 1545, 1866, 1872, 1878
MspA1I CMGCKG 3 cut(s) 71, 290, 1634
MspI CCGG 1 cut(s) 835
MspR9I CCNGG 1 cut(s) 1101
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 1 cut(s) 1101
MvnI CGCG 2 cut(s) 1616, 1853
MwoI GCNNNNNNNGC 8 cut(s) 287, 755, 914, 998, 1040, 1118, 1286, 1643
NdeII GATC 7 cut(s) 13, 244, 950, 1107, 1438, 1465, 1909
NlaIV GGNNCC 2 cut(s) 15, 625
NmuCI GTSAC 6 cut(s) 76, 172, 346, 367, 1678, 1843
NsbI TGCGCA 1 cut(s) 1822
OliI CACNNNNGTG 1 cut(s) 868
PaeR7I CTCGAG 1 cut(s) 2046
PagI TCATGA 2 cut(s) 1798, 1912
PceI AGGCCT 1 cut(s) 1073
PciSI GCTCTTC 1 cut(s) 1146
PcsI WCGNNNNNNNCGW 2 cut(s) 43, 1434
PctI GAATGC 1 cut(s) 1351
PdmI GAANNNNTTC 1 cut(s) 1656
PfeI GAWTC 6 cut(s) 515, 771, 923, 1432, 2038, 2089
PflMI CCANNNNNTGG 1 cut(s) 1535
PleI GAGTC 4 cut(s) 868, 1258, 1522, 1710
PmaCI CACGTG 1 cut(s) 899
PmlI CACGTG 1 cut(s) 899
PpsI GAGTC 4 cut(s) 868, 1258, 1522, 1710
Ppu21I YACGTR 2 cut(s) 899, 1133
PpuMI RGGWCCY 2 cut(s) 2051, 2149
Psp124BI GAGCTC 1 cut(s) 946
Psp5II RGGWCCY 2 cut(s) 2051, 2149
Psp6I CCWGG 1 cut(s) 1099
PspCI CACGTG 1 cut(s) 899
PspGI CCWGG 1 cut(s) 1099
PspN4I GGNNCC 2 cut(s) 15, 625
PspPI GGNCC 6 cut(s) 610, 623, 1090, 1748, 2051, 2149
PspPPI RGGWCCY 2 cut(s) 2051, 2149
PspXI VCTCGAGB 1 cut(s) 2046
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 3 cut(s) 13, 950, 1465
PvuII CAGCTG 3 cut(s) 71, 290, 1634
RsaI GTAC 8 cut(s) 481, 844, 1665, 1805, 1975, 2029, 2138, 2164
RsaNI GTAC 8 cut(s) 480, 843, 1664, 1804, 1974, 2028, 2137, 2163
RseI CAYNNNNRTG 6 cut(s) 858, 868, 1545, 1866, 1872, 1878
SacI GAGCTC 1 cut(s) 946
SapI GCTCTTC 1 cut(s) 1146
Sau3AI GATC 7 cut(s) 13, 244, 950, 1107, 1438, 1465, 1909
Sau96I GGNCC 6 cut(s) 610, 623, 1090, 1748, 2051, 2149
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 4 cut(s) 868, 1259, 1523, 1710
ScrFI CCNGG 1 cut(s) 1101
SduI GDGCHC 6 cut(s) 121, 898, 946, 1158, 1885, 1996
SfaNI GCATC 5 cut(s) 460, 850, 979, 1252, 1766
SfcI CTRYAG 2 cut(s) 1172, 2055
Sfr274I CTCGAG 1 cut(s) 2046
SinI GGWCC 4 cut(s) 623, 1748, 2051, 2149
SlaI CTCGAG 1 cut(s) 2046
SmiMI CAYNNNNRTG 6 cut(s) 858, 868, 1545, 1866, 1872, 1878
SmlI CTYRAG 2 cut(s) 1445, 2046
SmoI CTYRAG 2 cut(s) 1445, 2046
SnaBI TACGTA 1 cut(s) 1133
SpeI ACTAGT 1 cut(s) 788
Sse9I AATT 6 cut(s) 969, 1290, 1506, 1551, 1707, 1830
SseBI AGGCCT 1 cut(s) 1073
SsiI CCGC 3 cut(s) 551, 1339, 1851
SspI AATATT 1 cut(s) 1774
SspMI CTAG 6 cut(s) 113, 483, 789, 930, 947, 1479
SstI GAGCTC 1 cut(s) 946
StuI AGGCCT 1 cut(s) 1073
StyD4I CCNGG 1 cut(s) 1099
StyI CCWWGG 1 cut(s) 993
TaaI ACNGT 5 cut(s) 421, 545, 847, 1765, 1808
TaiI ACGT 6 cut(s) 666, 901, 984, 1127, 1135, 1739
TaqI TCGA 5 cut(s) 270, 940, 1255, 1343, 2047
TasI AATT 6 cut(s) 969, 1290, 1506, 1551, 1707, 1830
TatI WGTACW 5 cut(s) 479, 1663, 1803, 1973, 2136
TauI GCSGC 1 cut(s) 1853
TfiI GAWTC 6 cut(s) 515, 771, 923, 1432, 2038, 2089
TseFI GTSAC 6 cut(s) 76, 172, 346, 367, 1678, 1843
TseI GCWGC 9 cut(s) 130, 234, 287, 746, 758, 1112, 1485, 1634, 1952
Tsp45I GTSAC 6 cut(s) 76, 172, 346, 367, 1678, 1843
TspGWI ACGGA 2 cut(s) 386, 1637
Van91I CCANNNNNTGG 1 cut(s) 1535
VneI GTGCAC 2 cut(s) 894, 1992
VpaK11BI GGWCC 4 cut(s) 623, 1748, 2051, 2149
XapI RAATTY 2 cut(s) 969, 1551
XbaI TCTAGA 1 cut(s) 929
XhoI CTCGAG 1 cut(s) 2046
XmiI GTMKAC 1 cut(s) 2115
XmnI GAANNNNTTC 1 cut(s) 1656
XspI CTAG 6 cut(s) 113, 483, 789, 930, 947, 1479
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.