Rmu_co8474891.1_g000001

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8474891.1
OG0000993
Physical Location & Seq
Reverse (-)
1 .. 1790
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8474891.1_g000001.1.cds

Sequence Viewer

Length: 494 bp
atggcaaagcgcattgttcatgatgcaaagacagattatcctgcagcctgtaatgcaatggaaacacttcttgtacaccaggatttgtttaacaatggtcagcttaatgagataatcacacaactccgagagtcaggtgttactttgtatggtggaccacatgcagcagttttgttaaatctttcagagacaagcacattgcatcacgagtacagttcaatggcttgcacagttgaaattgtagatgatgtgcatgatgcagttgaccacattcatgtatatggaagtggtcacactgaatgcattgttactgaagatgatgaagttgctgaatatttcttaagccaagttgacagcgctgcagtatttcacaatgcaagtacacgattctgcgacggagctcgctttggtcttggtgcagaggttgggataagtacaagcaggattcacgctcgaggtcctgtaggagttgaaggactgctaacaaccagatg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

164

Amino Acids

17.76

Weight (kDa)

4.97

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 4 cut(s) 75, 212, 382, 436
AfeI AGCGCT 1 cut(s) 358
AflII CTTAAG 1 cut(s) 340
AgsI TTSAA 3 cut(s) 219, 236, 473
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 2 cut(s) 103, 401
AluI AGCT 2 cut(s) 103, 401
Alw21I GWGCWC 1 cut(s) 403
Alw26I GTCTC 1 cut(s) 182
Ama87I CYCGRG 1 cut(s) 453
Aor51HI AGCGCT 1 cut(s) 358
ApeKI GCWGC 3 cut(s) 44, 164, 359
Asp700I GAANNNNTTC 1 cut(s) 66
AspLEI GCGC 2 cut(s) 12, 359
AspS9I GGNCC 2 cut(s) 155, 458
AvaI CYCGRG 1 cut(s) 453
AvaII GGWCC 2 cut(s) 155, 458
BanII GRGCYC 1 cut(s) 403
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 3 cut(s) 56, 176, 346
BciT130I CCWGG 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 182
BfmI CTRYAG 3 cut(s) 42, 360, 462
BfoI RGCGCY 1 cut(s) 360
BfrI CTTAAG 1 cut(s) 340
BisI GCNGC 3 cut(s) 45, 165, 360
BlsI GCNGC 3 cut(s) 46, 166, 361
Bme1390I CCNGG 1 cut(s) 80
Bme18I GGWCC 2 cut(s) 155, 458
BmeT110I CYCGRG 1 cut(s) 453
BmgT120I GGNCC 2 cut(s) 155, 458
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 3 cut(s) 13, 211, 247
Bse3DI GCAATG 2 cut(s) 63, 197
BseBI CCWGG 1 cut(s) 80
BseMI GCAATG 2 cut(s) 63, 197
BseXI GCAGC 3 cut(s) 56, 176, 346
BsgI GTGCAG 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 403
BsiHKCI CYCGRG 1 cut(s) 453
BsmAI GTCTC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 453
Bsp1286I GDGCHC 1 cut(s) 403
Bsp1407I TGTACA 1 cut(s) 73
BspHI TCATGA 1 cut(s) 19
BspMAI CTGCAG 2 cut(s) 46, 364
BspTI CTTAAG 1 cut(s) 340
BsrDI GCAATG 2 cut(s) 63, 197
BsrGI TGTACA 1 cut(s) 73
BssSI CACGAG 1 cut(s) 206
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 215, 232
BstAFI CTTAAG 1 cut(s) 340
BstAUI TGTACA 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 226, 403
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 2 cut(s) 12, 359
BstMAI GTCTC 1 cut(s) 182
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 3 cut(s) 42, 360, 462
BstV1I GCAGC 3 cut(s) 56, 176, 346
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 226, 403
CciI TCATGA 1 cut(s) 19
CfoI GCGC 2 cut(s) 12, 359
Cfr13I GGNCC 2 cut(s) 155, 458
Csp6I GTAC 4 cut(s) 74, 211, 381, 435
CviAII CATG 4 cut(s) 20, 161, 254, 275
CviJI RGCY 5 cut(s) 47, 103, 224, 345, 401
CviKI_1 RGCY 5 cut(s) 47, 103, 224, 345, 401
CviQI GTAC 4 cut(s) 74, 211, 381, 435
Ecl136II GAGCTC 1 cut(s) 401
Eco24I GRGCYC 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 155, 458
Eco47III AGCGCT 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 333
Eco88I CYCGRG 1 cut(s) 453
EcoICRI GAGCTC 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 305
EcoT38I GRGCYC 1 cut(s) 403
FaeI CATG 4 cut(s) 23, 164, 257, 278
FaiI YATR 7 cut(s) 21, 150, 162, 255, 276, 280, 282
FatI CATG 4 cut(s) 19, 160, 253, 274
Fnu4HI GCNGC 3 cut(s) 45, 165, 360
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 3 cut(s) 45, 165, 360
GlaI GCGC 2 cut(s) 11, 358
GluI GCNGC 3 cut(s) 45, 165, 360
HaeII RGCGCY 1 cut(s) 360
HhaI GCGC 2 cut(s) 12, 359
Hin1II CATG 4 cut(s) 23, 164, 257, 278
Hin6I GCGC 2 cut(s) 10, 357
HinP1I GCGC 2 cut(s) 10, 357
HincII GTYRAC 2 cut(s) 265, 352
HindII GTYRAC 2 cut(s) 265, 352
HinfI GANTC 3 cut(s) 131, 387, 445
Hpy166II GTNNAC 5 cut(s) 76, 155, 265, 352, 383
Hpy188I TCNGA 2 cut(s) 128, 187
Hpy188III TCNNGA 2 cut(s) 20, 206
Hpy8I GTNNAC 5 cut(s) 76, 155, 265, 352, 383
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 1 cut(s) 467
HpyCH4III ACNGT 2 cut(s) 215, 232
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
Hsp92II CATG 4 cut(s) 23, 164, 257, 278
HspAI GCGC 2 cut(s) 10, 357
LmnI GCTCC 1 cut(s) 398
LpnPI CCDG 7 cut(s) 54, 61, 65, 92, 120, 427, 474
Lsp1109I GCAGC 3 cut(s) 56, 176, 346
LweI GCATC 3 cut(s) 13, 211, 247
MaeIII GTNAC 3 cut(s) 139, 290, 307
MboII GAAGA 1 cut(s) 326
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 1 cut(s) 237
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 2 cut(s) 415, 449
Mph1103I ATGCAT 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 4 cut(s) 90, 105, 176, 341
MslI CAYNNNNRTG 2 cut(s) 273, 279
MspCI CTTAAG 1 cut(s) 340
MspR9I CCNGG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 305
MvaI CCWGG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 53
NlaIII CATG 4 cut(s) 23, 164, 257, 278
NmuCI GTSAC 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 305
NspI RCATGY 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 453
PagI TCATGA 1 cut(s) 19
PctI GAATGC 1 cut(s) 305
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 387, 445
PkrI GCNGC 3 cut(s) 46, 166, 361
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 458
Psp124BI GAGCTC 1 cut(s) 403
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspPI GGNCC 2 cut(s) 155, 458
PspPPI RGGWCCY 1 cut(s) 458
PspXI VCTCGAGB 1 cut(s) 453
PstI CTGCAG 2 cut(s) 46, 364
RsaI GTAC 4 cut(s) 75, 212, 382, 436
RsaNI GTAC 4 cut(s) 74, 211, 381, 435
RseI CAYNNNNRTG 2 cut(s) 273, 279
SacI GAGCTC 1 cut(s) 403
SaqAI TTAA 4 cut(s) 90, 105, 176, 341
SatI GCNGC 3 cut(s) 45, 165, 360
Sau96I GGNCC 2 cut(s) 155, 458
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 403
SetI ASST 5 cut(s) 105, 139, 403, 426, 460
SfaNI GCATC 3 cut(s) 13, 211, 247
SfcI CTRYAG 3 cut(s) 42, 360, 462
Sfr274I CTCGAG 1 cut(s) 453
SinI GGWCC 2 cut(s) 155, 458
SlaI CTCGAG 1 cut(s) 453
SmiMI CAYNNNNRTG 2 cut(s) 273, 279
SmlI CTYRAG 2 cut(s) 340, 453
SmoI CTYRAG 2 cut(s) 340, 453
Sse9I AATT 1 cut(s) 237
SspI AATATT 1 cut(s) 335
SstI GAGCTC 1 cut(s) 403
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 2 cut(s) 215, 232
TaqI TCGA 1 cut(s) 454
TasI AATT 1 cut(s) 237
TatI WGTACW 4 cut(s) 73, 210, 380, 434
TfiI GAWTC 2 cut(s) 387, 445
Tru1I TTAA 4 cut(s) 90, 105, 176, 341
Tru9I TTAA 4 cut(s) 90, 105, 176, 341
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 3 cut(s) 44, 164, 359
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 3 cut(s) 8, 263, 336
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 155, 458
XceI RCATGY 1 cut(s) 164
XhoI CTCGAG 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.