MD00G1141100.v1.1

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000993
Physical Location & Seq
Forward (+)
30840065 .. 30845349
5285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1141100.v1.1.491

Sequence Viewer

Length: 2043 bp
ATGTCGAGTAAAACTCCATTGGGCAAGCAAACCATGGACTCTGTGGATCCTACGAGAGCTTTTGTCAAGAATGTCAAGCGTATCGTTGTTAAGGTCGGAACAGCTGTGGTCACTCGTGGTGATGGAAGATTAGCATTGGGAAGACTAGGCGCTCTGTGTGAGCAGCTTAAAGATATAAATTCCCAGGGCTATGAGGTTATTTTGGTGACTTCAGGAGCTGTTGGTCTTGGCCGTCAAAGGCTAAGATTCCGGAGATTGGCAAATAGCAGCGTGTTTAGGGATGAAGCTTTCCGAAAGCAGCTTTCTGAGACTGTAAAGTCGCTGTTAAAACTGAGGGTTGTTCCTATCTTCAATGAAAATGATGCTGTTAGTACTAGGAAAGCTCCATACGAGGATTCTTCTGGCATATTTTGGGACAATGACAGTTTGGCTGGGCTATTGGCTCTGGAACTAAAAGCTGACCTTCTAGTTTTGTTAAGCGATGTAGAGGGCCTCTATAGCGGCCCTCCTACTGACCCGAAATCAAAGTTAATCCATACTTATGTGAAAGAGAAACATCAGGGAGAAATAACTTTTGGAGACAAATCAAGGGTGGGCAGAGGCGGTATGACTGCTAAAGTCAATGCTGCTGTATGTGCAGCTTATGGTGGAATCCCTGTTGTTATCACTAGCGGTTATGCAGACAACATAAAGAAAGTGCTTCGTGGAGACCGGATAGGCACTGTATTTCATCAAGATGCACATTTGTGGACTCTAGTTAAGGAAGAAGGTGCACGTGACATGGCTGTTGCAGCGCGGGAATCATCTAGACAACTTCGAGCTCTAGGATCTCAAGAGAGGAGGAAAATTTTGCTTGACGTAGCTGATGCCTTGGAGGCAAATGAAGGTGAAATTTTAGCTGCGAATGAAGCTGATGTTGCTGCTGCGCAGGATGATAGATATGAAAAGGCCCTAATATCGCGTTTAGCCCTAAAGCCTGGGAAGATTTCTGCTCTTGCAAAGTCCATACGTAAACTTGCAGACATGGAAGAGCCCATTGGCCGTGTTCTAAAGAAGACCGAGCTTGCAGAAGGGCTTGTCTTGGAGAAACTGTCTTGTCCCTTGGGTGTTCTCTTAGTTGTGTTTGAGTCTCGACCGGATGCCCTTGTTCAGATAGCTTCATTAGCAATTCAAAGTAGGAACGGTCTTCTGCTTAAAGGTGGGAAAGAAGCCAAGCGATCAAATGCAATCTTGCACAAGGTCATCACCTCAGCAATCCCAGAACCTGTTGGCGGCAAACTTATCGGACTTGTGAATACAAGAGATGAGATTCCTGACCTGCTCAAGCTCGATAATGTGATTGATCTTGTCATCCCTAGAGGCAGCAATAAGCTTGTTTCTCAAATTAAGGAGTCAACTAAGATACCTGTTCTTGGTCATGCCGATGGGATTTGTCACGTTTATGTTGACAAGTCTGCTAATCTGGATATGGCGAAGCGCATTGTTCTGGATGCGAAAGTAGACTACCCTGCTGCCTGCAATGCAATGGAAACACTTCTCGTACACAAGGATTTGTTGAGTGGCGATGGCGGGTTTAAGCAGATAATTTCAGAACTCCAGGTTTCAGATGTTACCTTATATGGTGGACCTAGGGCAGCAACTGCGTTAAACCTTCCAGAGACAAACTCATTCCATTACGAGTACAGTTCATTGGCTTGCTCGGTTGAAATCGTTGATGATGTAAACGCTGCGATTGACCACATTCATGAGCATGGAAGTGCTCATACCGATTGCATTGTGGCTGAAGATCGTCAAGTTGCTGAAACTTTCCTAACTCAAGTCGACAGTGCTGCAGTCTTTCATAATGCGAGTACAAGGTTTTGTGATGGAGCTCGCTTTGGACTCGGTGCAGAAGTCGGGATAAGTACGAGCAGGATTCACGCGCGAGGTCCTGTAGGAGTTGAAGGATTGCTAACAACAAGATGGGTTCTGAGAGGGAACGGGCAGATTGTAGACGCGGATAGAGGGGTCGAGTACACACACAAGGACCTGCTTAATTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

681

Amino Acids

73.48

Weight (kDa)

7.63

Isoelectric Point (pI)

29.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 26 - 78 6.9e-06 Amino acid kinase family
AA_kinase PF00696 93 - 224 1.9e-18 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 316
Acc16I TGCGCA 1 cut(s) 927
Acc36I ACCTGC 2 cut(s) 1326, 2037
AccI GTMKAC 3 cut(s) 1500, 1821, 1992
AccII CGCG 5 cut(s) 796, 961, 1922, 1924, 1997
AccIII TCCGGA 1 cut(s) 249
AciI CCGC 7 cut(s) 501, 603, 672, 796, 1272, 1569, 1997
AclWI GGATC 3 cut(s) 41, 54, 835
AcoI YGGCCR 2 cut(s) 229, 1039
AcsI RAATTY 3 cut(s) 178, 846, 891
AcuI CTGAAG 2 cut(s) 195, 1803
AcvI CACGTG 1 cut(s) 776
AfaI GTAC 6 cut(s) 373, 1542, 1682, 1852, 1906, 2015
AfiI CCNNNNNNNGG 3 cut(s) 256, 1271, 1412
AgsI TTSAA 4 cut(s) 352, 1172, 1706, 1943
AjnI CCWGG 3 cut(s) 183, 976, 1596
AjuI GAANNNNNNNTTGG 4 cut(s) 558, 590, 1020, 1052
AleI CACNNNNGTG 1 cut(s) 745
Alw21I GWGCWC 4 cut(s) 775, 823, 1762, 1873
Alw26I GTCTC 5 cut(s) 302, 573, 702, 1134, 1652
Alw44I GTGCAC 1 cut(s) 771
AlwI GGATC 3 cut(s) 41, 54, 835
AlwNI CAGNNNCTG 3 cut(s) 218, 1265, 1640
Aor13HI TCCGGA 1 cut(s) 249
AoxI GGCC 5 cut(s) 229, 490, 502, 948, 1039
ApaLI GTGCAC 1 cut(s) 771
ApoI RAATTY 3 cut(s) 178, 846, 891
Asp700I GAANNNNTTC 1 cut(s) 1533
AspA2I CCTAGG 1 cut(s) 1628
AspLEI GCGC 5 cut(s) 152, 796, 928, 1479, 1924
AspS9I GGNCC 6 cut(s) 490, 503, 949, 1625, 1928, 2026
AsuHPI GGTGA 4 cut(s) 131, 217, 899, 1237
AvaII GGWCC 3 cut(s) 1625, 1928, 2026
AvrII CCTAGG 1 cut(s) 1628
BaeGI GKGCMC 1 cut(s) 775
BamHI GGATCC 1 cut(s) 46
BanII GRGCYC 3 cut(s) 823, 1035, 1873
BarI GAAGNNNNNNTAC 2 cut(s) 1747, 1779
BauI CACGAG 1 cut(s) 114
BbrPI CACGTG 1 cut(s) 776
BbsI GAAGAC 3 cut(s) 148, 1061, 1178
Bbv12I GWGCWC 4 cut(s) 775, 823, 1762, 1873
BbvCI CCTCAGC 1 cut(s) 1249
BccI CCATC 5 cut(s) 116, 1418, 1559, 1859, 1956
BceAI ACGGC 2 cut(s) 216, 1026
BcgI CGANNNNNNTGC 4 cut(s) 1264, 1298, 1811, 1845
BciT130I CCWGG 3 cut(s) 185, 978, 1598
BcoDI GTCTC 5 cut(s) 302, 573, 702, 1134, 1652
BfaI CTAG 9 cut(s) 146, 375, 467, 669, 755, 807, 824, 1356, 1629
BfmI CTRYAG 3 cut(s) 496, 1830, 1932
BfoI RGCGCY 1 cut(s) 153
BfuAI ACCTGC 2 cut(s) 1326, 2037
BglI GCCNNNNNGGC 1 cut(s) 875
BlnI CCTAGG 1 cut(s) 1628
BmcAI AGTACT 1 cut(s) 373
Bme1390I CCNGG 3 cut(s) 185, 978, 1598
Bme18I GGWCC 3 cut(s) 1625, 1928, 2026
BmgT120I GGNCC 6 cut(s) 490, 503, 949, 1625, 1928, 2026
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 3 cut(s) 185, 978, 1598
BmsI GCATC 5 cut(s) 352, 727, 856, 1129, 1480
BpiI GAAGAC 3 cut(s) 148, 1061, 1178
BplI GAGNNNNNCTC 3 cut(s) 30, 1649, 1681
BpmI CTGGAG 1 cut(s) 1580
Bpu10I CCTNAGC 1 cut(s) 1249
BpuEI CTTGAG 3 cut(s) 816, 1307, 1800
BsaAI YACGTR 2 cut(s) 776, 1010
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 7 cut(s) 33, 183, 184, 870, 977, 1101, 1628
BsaWI WCCGGW 3 cut(s) 249, 711, 1135
BsaXI ACNNNNNCTCC 2 cut(s) 1076, 1106
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 1271, 1412
Bse3DI GCAATG 2 cut(s) 1525, 1530
BseAI TCCGGA 1 cut(s) 249
BseBI CCWGG 3 cut(s) 185, 978, 1598
BseDI CCNNGG 7 cut(s) 33, 183, 184, 870, 977, 1101, 1628
BseGI GGATG 5 cut(s) 286, 937, 1144, 1350, 1495
BseLI CCNNNNNNNGG 3 cut(s) 256, 1271, 1412
BseMI GCAATG 2 cut(s) 1525, 1530
BseMII CTCAG 4 cut(s) 297, 323, 1263, 1961
BseRI GAGGAG 1 cut(s) 853
BseSI GKGCMC 1 cut(s) 775
BseYI CCCAGC 1 cut(s) 431
BsgI GTGCAG 2 cut(s) 657, 1908
Bsh1236I CGCG 5 cut(s) 796, 961, 1922, 1924, 1997
Bsh1285I CGRYCG 1 cut(s) 1136
BshFI GGCC 5 cut(s) 231, 492, 504, 950, 1041
BsiEI CGRYCG 1 cut(s) 1136
BsiHKAI GWGCWC 4 cut(s) 775, 823, 1762, 1873
BsiSI CCGG 3 cut(s) 250, 712, 1136
BslFI GGGAC 2 cut(s) 428, 1083
BslI CCNNNNNNNGG 3 cut(s) 256, 1271, 1412
BsmAI GTCTC 5 cut(s) 302, 573, 702, 1134, 1652
BsmFI GGGAC 2 cut(s) 428, 1083
BsnI GGCC 5 cut(s) 231, 492, 504, 950, 1041
Bso31I GGTCTC 1 cut(s) 702
Bsp1286I GDGCHC 5 cut(s) 775, 823, 1035, 1762, 1873
Bsp13I TCCGGA 1 cut(s) 249
Bsp143I GATC 5 cut(s) 46, 827, 1217, 1342, 1786
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 7 cut(s) 501, 603, 672, 796, 1272, 1569, 1997
BspANI GGCC 5 cut(s) 231, 492, 504, 950, 1041
BspCNI CTCAG 4 cut(s) 298, 324, 1262, 1962
BspEI TCCGGA 1 cut(s) 249
BspFNI CGCG 5 cut(s) 796, 961, 1922, 1924, 1997
BspHI TCATGA 1 cut(s) 1744
BspLI GGNNCC 1 cut(s) 48
BspMAI CTGCAG 1 cut(s) 1834
BspMI ACCTGC 2 cut(s) 1326, 2037
BspPI GGATC 3 cut(s) 41, 54, 835
BspQI GCTCTTC 1 cut(s) 1023
BspTNI GGTCTC 1 cut(s) 702
BsrDI GCAATG 2 cut(s) 1525, 1530
BssECI CCNNGG 7 cut(s) 33, 183, 184, 870, 977, 1101, 1628
BssMI GATC 5 cut(s) 46, 827, 1217, 1342, 1786
BssSI CACGAG 1 cut(s) 114
BssT1I CCWWGG 4 cut(s) 33, 870, 1101, 1628
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 3 cut(s) 185, 978, 1598
Bst4CI ACNGT 7 cut(s) 313, 425, 724, 1092, 1184, 1685, 1826
Bst6I CTCTTC 1 cut(s) 1023
BstAPI GCANNNNNTGC 1 cut(s) 1640
BstBAI YACGTR 2 cut(s) 776, 1010
BstC8I GCNNGC 5 cut(s) 26, 1065, 1516, 1696, 1873
BstDEI CTNAG 7 cut(s) 242, 306, 332, 1114, 1249, 1398, 1970
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 5 cut(s) 286, 937, 1144, 1350, 1495
BstFNI CGCG 5 cut(s) 796, 961, 1922, 1924, 1997
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 5 cut(s) 152, 796, 928, 1479, 1924
BstKTI GATC 5 cut(s) 49, 830, 1220, 1345, 1789
BstMAI GTCTC 5 cut(s) 302, 573, 702, 1134, 1652
BstMBI GATC 5 cut(s) 46, 827, 1217, 1342, 1786
BstMCI CGRYCG 1 cut(s) 1136
BstMWI GCNNNNNNNGC 9 cut(s) 498, 635, 791, 875, 908, 917, 1163, 1520, 1640
BstNI CCWGG 3 cut(s) 185, 978, 1598
BstSCI CCNGG 3 cut(s) 183, 976, 1596
BstSFI CTRYAG 3 cut(s) 496, 1830, 1932
BstSLI GKGCMC 1 cut(s) 775
BstSNI TACGTA 1 cut(s) 1010
BstUI CGCG 5 cut(s) 796, 961, 1922, 1924, 1997
BstV2I GAAGAC 3 cut(s) 148, 1061, 1178
BstX2I RGATCY 2 cut(s) 46, 827
BstYI RGATCY 2 cut(s) 46, 827
BsuRI GGCC 5 cut(s) 231, 492, 504, 950, 1041
BtgI CCRYGG 1 cut(s) 33
BtgZI GCGATG 2 cut(s) 495, 1578
BtsCI GGATG 5 cut(s) 286, 937, 1144, 1350, 1495
BtsIMutI CAGTG 2 cut(s) 720, 1831
BveI ACCTGC 2 cut(s) 1326, 2037
Cac8I GCNNGC 5 cut(s) 26, 1065, 1516, 1696, 1873
CaiI CAGNNNCTG 3 cut(s) 218, 1265, 1640
CciI TCATGA 1 cut(s) 1744
CfoI GCGC 5 cut(s) 152, 796, 928, 1479, 1924
Cfr13I GGNCC 6 cut(s) 490, 503, 949, 1625, 1928, 2026
CseI GACGC 1 cut(s) 2003
Csp6I GTAC 6 cut(s) 372, 1541, 1681, 1851, 1905, 2014
CviAII CATG 6 cut(s) 34, 781, 1024, 1418, 1745, 1751
CviQI GTAC 6 cut(s) 372, 1541, 1681, 1851, 1905, 2014
DdeI CTNAG 7 cut(s) 242, 306, 332, 1114, 1249, 1398, 1970
DpnI GATC 5 cut(s) 48, 829, 1219, 1344, 1788
DpnII GATC 5 cut(s) 46, 827, 1217, 1342, 1786
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
EaeI YGGCCR 2 cut(s) 229, 1039
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
Ecl136II GAGCTC 2 cut(s) 821, 1871
Eco105I TACGTA 1 cut(s) 1010
Eco130I CCWWGG 4 cut(s) 33, 870, 1101, 1628
Eco24I GRGCYC 3 cut(s) 823, 1035, 1873
Eco31I GGTCTC 1 cut(s) 702
Eco47I GGWCC 3 cut(s) 1625, 1928, 2026
Eco53kI GAGCTC 2 cut(s) 821, 1871
Eco57I CTGAAG 2 cut(s) 195, 1803
Eco72I CACGTG 1 cut(s) 776
EcoICRI GAGCTC 2 cut(s) 821, 1871
EcoO109I RGGNCCY 4 cut(s) 490, 949, 1928, 2026
EcoRII CCWGG 3 cut(s) 183, 976, 1596
EcoT14I CCWWGG 4 cut(s) 33, 870, 1101, 1628
EcoT38I GRGCYC 3 cut(s) 823, 1035, 1873
ErhI CCWWGG 4 cut(s) 33, 870, 1101, 1628
FaeI CATG 6 cut(s) 37, 784, 1027, 1421, 1748, 1754
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FaqI GGGAC 2 cut(s) 428, 1083
FatI CATG 6 cut(s) 33, 780, 1023, 1417, 1744, 1750
FauI CCCGC 2 cut(s) 789, 1562
FblI GTMKAC 3 cut(s) 1500, 1821, 1992
FokI GGATG 5 cut(s) 293, 944, 1151, 1337, 1502
FriOI GRGCYC 3 cut(s) 823, 1035, 1873
FspBI CTAG 9 cut(s) 146, 375, 467, 669, 755, 807, 824, 1356, 1629
FspI TGCGCA 1 cut(s) 927
GlaI GCGC 5 cut(s) 151, 795, 927, 1478, 1923
GsaI CCCAGC 1 cut(s) 435
GsuI CTGGAG 1 cut(s) 1580
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 5 cut(s) 231, 492, 504, 950, 1041
HapII CCGG 3 cut(s) 250, 712, 1136
HgaI GACGC 1 cut(s) 2003
HhaI GCGC 5 cut(s) 152, 796, 928, 1479, 1924
Hin1II CATG 6 cut(s) 37, 784, 1027, 1421, 1748, 1754
Hin6I GCGC 5 cut(s) 150, 794, 926, 1477, 1922
HinP1I GCGC 5 cut(s) 150, 794, 926, 1477, 1922
HincII GTYRAC 3 cut(s) 1395, 1447, 1822
HindII GTYRAC 3 cut(s) 1395, 1447, 1822
HindIII AAGCTT 2 cut(s) 285, 1370
HpaII CCGG 3 cut(s) 250, 712, 1136
HphI GGTGA 4 cut(s) 131, 217, 899, 1237
Hpy188I TCNGA 8 cut(s) 98, 293, 307, 1152, 1286, 1591, 1606, 1971
HpyAV CCTTC 6 cut(s) 473, 761, 878, 1064, 1661, 1937
HpyCH4III ACNGT 7 cut(s) 313, 425, 724, 1092, 1184, 1685, 1826
HpyCH4IV ACGT 4 cut(s) 775, 858, 1009, 1437
HpyF10VI GCNNNNNNNGC 9 cut(s) 498, 635, 791, 875, 908, 917, 1163, 1520, 1640
HpyF3I CTNAG 7 cut(s) 242, 306, 332, 1114, 1249, 1398, 1970
HpySE526I ACGT 4 cut(s) 775, 858, 1009, 1437
Hsp92II CATG 6 cut(s) 37, 784, 1027, 1421, 1748, 1754
HspAI GCGC 5 cut(s) 150, 794, 926, 1477, 1922
Kpn2I TCCGGA 1 cut(s) 249
Kzo9I GATC 5 cut(s) 46, 827, 1217, 1342, 1786
LguI GCTCTTC 1 cut(s) 1023
LmnI GCTCC 3 cut(s) 215, 388, 1868
LweI GCATC 5 cut(s) 352, 727, 856, 1129, 1480
MaeI CTAG 9 cut(s) 146, 375, 467, 669, 755, 807, 824, 1356, 1629
MaeII ACGT 4 cut(s) 775, 858, 1009, 1437
MaeIII GTNAC 5 cut(s) 109, 205, 776, 1433, 1609
MalI GATC 5 cut(s) 48, 829, 1219, 1344, 1788
MboI GATC 5 cut(s) 46, 827, 1217, 1342, 1786
MflI RGATCY 2 cut(s) 46, 827
MhlI GDGCHC 5 cut(s) 775, 823, 1035, 1762, 1873
MluCI AATT 7 cut(s) 178, 846, 891, 1167, 1383, 1584, 2035
MlyI GAGTC 5 cut(s) 32, 745, 1136, 1400, 1875
MmeI TCCRAC 1 cut(s) 76
MroI TCCGGA 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 1533
MslI CAYNNNNRTG 8 cut(s) 540, 735, 745, 1422, 1440, 1743, 1749, 1755
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 3 cut(s) 250, 712, 1136
MspR9I CCNGG 3 cut(s) 185, 978, 1598
MvaI CCWGG 3 cut(s) 185, 978, 1598
MvnI CGCG 5 cut(s) 796, 961, 1922, 1924, 1997
MwoI GCNNNNNNNGC 9 cut(s) 498, 635, 791, 875, 908, 917, 1163, 1520, 1640
NcoI CCATGG 1 cut(s) 33
NdeII GATC 5 cut(s) 46, 827, 1217, 1342, 1786
NlaIII CATG 6 cut(s) 37, 784, 1027, 1421, 1748, 1754
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 4 cut(s) 109, 205, 776, 1433
NsbI TGCGCA 1 cut(s) 927
OliI CACNNNNGTG 1 cut(s) 745
PagI TCATGA 1 cut(s) 1744
PasI CCCWGGG 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 1023
PdmI GAANNNNTTC 1 cut(s) 1533
PfeI GAWTC 6 cut(s) 246, 395, 651, 800, 1309, 1915
PleI GAGTC 5 cut(s) 32, 745, 1135, 1399, 1875
PmaCI CACGTG 1 cut(s) 776
PmlI CACGTG 1 cut(s) 776
PpsI GAGTC 5 cut(s) 32, 745, 1135, 1399, 1875
Ppu21I YACGTR 2 cut(s) 776, 1010
PpuMI RGGWCCY 2 cut(s) 1928, 2026
Psp124BI GAGCTC 2 cut(s) 823, 1873
Psp5II RGGWCCY 2 cut(s) 1928, 2026
Psp6I CCWGG 3 cut(s) 183, 976, 1596
PspCI CACGTG 1 cut(s) 776
PspFI CCCAGC 1 cut(s) 431
PspGI CCWGG 3 cut(s) 183, 976, 1596
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 6 cut(s) 490, 503, 949, 1625, 1928, 2026
PspPPI RGGWCCY 2 cut(s) 1928, 2026
PstI CTGCAG 1 cut(s) 1834
PstNI CAGNNNCTG 3 cut(s) 218, 1265, 1640
PsuI RGATCY 2 cut(s) 46, 827
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 6 cut(s) 373, 1542, 1682, 1852, 1906, 2015
RsaNI GTAC 6 cut(s) 372, 1541, 1681, 1851, 1905, 2014
RseI CAYNNNNRTG 8 cut(s) 540, 735, 745, 1422, 1440, 1743, 1749, 1755
SacI GAGCTC 2 cut(s) 823, 1873
SalI GTCGAC 1 cut(s) 1820
SapI GCTCTTC 1 cut(s) 1023
Sau3AI GATC 5 cut(s) 46, 827, 1217, 1342, 1786
Sau96I GGNCC 6 cut(s) 490, 503, 949, 1625, 1928, 2026
ScaI AGTACT 1 cut(s) 373
SchI GAGTC 5 cut(s) 32, 745, 1136, 1400, 1875
ScrFI CCNGG 3 cut(s) 185, 978, 1598
SduI GDGCHC 5 cut(s) 775, 823, 1035, 1762, 1873
SfaNI GCATC 5 cut(s) 352, 727, 856, 1129, 1480
SfcI CTRYAG 3 cut(s) 496, 1830, 1932
SinI GGWCC 3 cut(s) 1625, 1928, 2026
SmiMI CAYNNNNRTG 8 cut(s) 540, 735, 745, 1422, 1440, 1743, 1749, 1755
SmlI CTYRAG 3 cut(s) 831, 1322, 1815
SmoI CTYRAG 3 cut(s) 831, 1322, 1815
SnaBI TACGTA 1 cut(s) 1010
Sse9I AATT 7 cut(s) 178, 846, 891, 1167, 1383, 1584, 2035
SsiI CCGC 7 cut(s) 501, 603, 672, 796, 1272, 1569, 1997
SspMI CTAG 9 cut(s) 146, 375, 467, 669, 755, 807, 824, 1356, 1629
SstI GAGCTC 2 cut(s) 823, 1873
StyD4I CCNGG 3 cut(s) 183, 976, 1596
StyI CCWWGG 4 cut(s) 33, 870, 1101, 1628
TaaI ACNGT 7 cut(s) 313, 425, 724, 1092, 1184, 1685, 1826
TaiI ACGT 4 cut(s) 778, 861, 1012, 1440
TaqI TCGA 6 cut(s) 5, 817, 1132, 1329, 1821, 2010
TaqII GACCGA 1 cut(s) 1073
TasI AATT 7 cut(s) 178, 846, 891, 1167, 1383, 1584, 2035
TatI WGTACW 4 cut(s) 371, 1680, 1850, 2013
TauI GCSGC 2 cut(s) 504, 1275
TfiI GAWTC 6 cut(s) 246, 395, 651, 800, 1309, 1915
TscAI CASTG 2 cut(s) 727, 1831
TseFI GTSAC 4 cut(s) 109, 205, 776, 1433
Tsp45I GTSAC 4 cut(s) 109, 205, 776, 1433
TspRI CASTG 2 cut(s) 727, 1831
VneI GTGCAC 1 cut(s) 771
VpaK11BI GGWCC 3 cut(s) 1625, 1928, 2026
XapI RAATTY 3 cut(s) 178, 846, 891
XbaI TCTAGA 1 cut(s) 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XmaJI CCTAGG 1 cut(s) 1628
XmiI GTMKAC 3 cut(s) 1500, 1821, 1992
XmnI GAANNNNTTC 1 cut(s) 1533
XspI CTAG 9 cut(s) 146, 375, 467, 669, 755, 807, 824, 1356, 1629
ZrmI AGTACT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.