Rroxscaffold_6G00415250

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000993
Physical Location & Seq
Reverse (-)
37478100 .. 37486311
8212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00415250.1

Sequence Viewer

Length: 1440 bp
ATGACCGCTAAAGTCAATGCTGCTGTGTGTGCTGCTTATGGTGGAATCCCGGTTGTTATCACTAGTGGCTATGCTAATGATAACATTATCAAAGTGCTTCAAGGAGAACGAATTGGTACTGTCTTTCATCAAGATGCACATTTGTGGACTCTGGTTAAAGAAGAATGTGCACGCGACTTGGCTGTAGCAGCACGGGAATGCTCTAGAAAGCTTCGAGCTCTAAACTCTCAGGATAGGAGGCAAATATTGCTCGATGTAGCTGATGCCTTGGAGGCAAATGAAAGTGACATTTTAGCTGAAAATGAAGCTGATATTGCAGCTGCGCAGGAAGCTGGATATGATGGGGCACTAATAGCTCGGTTGGCCCTAAAGCCTGGGAAGATTTCTGCACTTGCAAAATCTATGCGTCAACTTGCAGACATGGAAGAGCCCATTGGCCAGGTCTTGCAGAAAACAGAGGTTGCAGATGGACTTGTCTTAGAGAAAGTGACTTGTCCCTTGGGTGTTCTTTTGGTTGTTTTTGAGTCTCGACCTGATGCCCTGGTTCAGATAGCTTCATTAGCAATTCAAAGTCGGAATGGTCTTCTGCTTAAAGGTGGGAAAGAAGCCAAGAGGTCAAATGCTATCTTGCACAAGGTCATCACCAAAGCAATCCCAGATACTGTTGGTGGCGAGCTTATTGGACTTGTAACTTCAAGAGATGATATTCCTGATCTGCTGAAGCTCGACGATGTGATAGATCTTGTCATCCCTAGAGGCAGTAATAAACTCGTTTCTCAAATCAAGGAGTCGACAAAGATACCTGTTCTTGGTCATGCTGATGGGATCTGCCATGTTTATATCGATAAGTCAGCTAATTTGGAGATGGCAAAGCGCATTGTTCATGATGCAAAGACAGATTATCCTGCAGCCTGTAATGCAATGGAAACACTTCTTGTACACCAGGATTTGTTTAACAATGGTCAGCTTAATGAGATAATCACACAACTCCGACAGTCAGGTGTTACTTTATATGGTGGACCACATGCAGCAGTTTTGTTAAATCTTTCAGAGACAAGCACATTGCATCACGAGTACAGTTCAATGGCTTGCACAGTTGAAATTGTAGATGATGTGCATGATGCAGTTGACCACATTCATGTATATGGAAGTGGTCACACTGAATGCATTGTTACTGAAGATGATGAAGTTGCTGAATATTTCTTAAGCCAAGTTGACAGCGCTGCAGTGTTTCACAATGCAAGTACACGATTCTGCGACGGAGCTCGCTTTGGTCTTGGTGCAGAGGTTGGGATAAGTACAAGCAGGATTCACGCTCGAGGTCCTGTAGGAGTTGAAGGACTGCTAACAACCAGATGGACTCTGAGGGGGAAAGGGCAGATCGTGGATGGTGATAATGGGGTCGAATACACACACAAGAATTTACTTAAAAGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

479

Amino Acids

51.56

Weight (kDa)

5.4

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldedh PF00171 53 - 320 7.8e-06 Aldehyde dehydrogenase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 324
AccI GTMKAC 1 cut(s) 791
AccII CGCG 1 cut(s) 174
AciI CCGC 1 cut(s) 6
AclWI GGATC 1 cut(s) 833
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 1 cut(s) 1420
AcuI CTGAAG 2 cut(s) 740, 1197
AfaI GTAC 5 cut(s) 118, 939, 1076, 1246, 1300
AfeI AGCGCT 1 cut(s) 1222
AfiI CCNNNNNNNGG 1 cut(s) 809
AflII CTTAAG 1 cut(s) 1204
AgsI TTSAA 6 cut(s) 101, 569, 696, 1083, 1100, 1337
AhlI ACTAGT 1 cut(s) 62
AjnI CCWGG 4 cut(s) 373, 438, 540, 942
AjuI GAANNNNNNNTTGG 2 cut(s) 417, 449
AleI CACNNNNGTG 1 cut(s) 142
Alw21I GWGCWC 3 cut(s) 172, 220, 1267
Alw26I GTCTC 2 cut(s) 531, 1046
Alw44I GTGCAC 1 cut(s) 168
AlwI GGATC 1 cut(s) 833
AlwNI CAGNNNCTG 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 1317
Aor51HI AGCGCT 1 cut(s) 1222
AoxI GGCC 2 cut(s) 363, 436
ApaLI GTGCAC 1 cut(s) 168
ApeKI GCWGC 8 cut(s) 20, 32, 188, 317, 320, 908, 1028, 1223
ApoI RAATTY 1 cut(s) 1420
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 3 cut(s) 325, 876, 1223
AspS9I GGNCC 3 cut(s) 364, 1019, 1322
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 2 cut(s) 634, 1403
AvaI CYCGRG 1 cut(s) 1317
AvaII GGWCC 2 cut(s) 1019, 1322
BaeGI GKGCMC 2 cut(s) 172, 349
BalI TGGCCA 1 cut(s) 438
BanII GRGCYC 3 cut(s) 220, 432, 1267
BauI CACGAG 1 cut(s) 1070
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 3 cut(s) 172, 220, 1267
BbvI GCAGC 8 cut(s) 7, 19, 200, 307, 329, 920, 1040, 1210
BccI CCATC 6 cut(s) 335, 461, 815, 859, 1350, 1382
BciT130I CCWGG 4 cut(s) 375, 440, 542, 944
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 2 cut(s) 531, 1046
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 3 cut(s) 63, 204, 753
BfmI CTRYAG 4 cut(s) 183, 906, 1224, 1326
BfoI RGCGCY 1 cut(s) 1224
BfrI CTTAAG 1 cut(s) 1204
BglI GCCNNNNNGGC 1 cut(s) 272
BglII AGATCT 1 cut(s) 739
BisI GCNGC 8 cut(s) 21, 33, 189, 318, 321, 909, 1029, 1224
BlsI GCNGC 8 cut(s) 22, 34, 190, 319, 322, 910, 1030, 1225
Bme1390I CCNGG 5 cut(s) 50, 375, 440, 542, 944
Bme18I GGWCC 2 cut(s) 1019, 1322
BmeT110I CYCGRG 1 cut(s) 1317
BmgT120I GGNCC 3 cut(s) 364, 1019, 1322
BmrFI CCNGG 5 cut(s) 50, 375, 440, 542, 944
BmsI GCATC 6 cut(s) 124, 253, 526, 877, 1075, 1111
BpiI GAAGAC 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 50
Bsa29I ATCGAT 1 cut(s) 843
BsaJI CCNNGG 4 cut(s) 267, 374, 498, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 809
Bse3DI GCAATG 2 cut(s) 927, 1061
BseBI CCWGG 4 cut(s) 375, 440, 542, 944
BseCI ATCGAT 1 cut(s) 843
BseDI CCNNGG 4 cut(s) 267, 374, 498, 540
BseGI GGATG 2 cut(s) 747, 1393
BseLI CCNNNNNNNGG 1 cut(s) 809
BseMI GCAATG 2 cut(s) 927, 1061
BseMII CTCAG 2 cut(s) 242, 1355
BseSI GKGCMC 2 cut(s) 172, 349
BseXI GCAGC 8 cut(s) 7, 19, 200, 307, 329, 920, 1040, 1210
BsgI GTGCAG 2 cut(s) 372, 1302
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 2 cut(s) 365, 438
BshVI ATCGAT 1 cut(s) 843
BsiHKAI GWGCWC 3 cut(s) 172, 220, 1267
BsiHKCI CYCGRG 1 cut(s) 1317
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 809
BsmAI GTCTC 2 cut(s) 531, 1046
BsmFI GGGAC 1 cut(s) 480
BsmI GAATGC 2 cut(s) 203, 1169
BsnI GGCC 2 cut(s) 365, 438
BsoBI CYCGRG 1 cut(s) 1317
Bsp1286I GDGCHC 5 cut(s) 172, 220, 349, 432, 1267
Bsp1407I TGTACA 1 cut(s) 937
Bsp143I GATC 4 cut(s) 712, 739, 825, 1380
BspACI CCGC 1 cut(s) 6
BspANI GGCC 2 cut(s) 365, 438
BspCNI CTCAG 2 cut(s) 241, 1356
BspDI ATCGAT 1 cut(s) 843
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 1 cut(s) 883
BspMAI CTGCAG 2 cut(s) 910, 1228
BspPI GGATC 1 cut(s) 833
BspQI GCTCTTC 1 cut(s) 420
BspTI CTTAAG 1 cut(s) 1204
BsrDI GCAATG 2 cut(s) 927, 1061
BsrGI TGTACA 1 cut(s) 937
BssECI CCNNGG 4 cut(s) 267, 374, 498, 540
BssMI GATC 4 cut(s) 712, 739, 825, 1380
BssSI CACGAG 1 cut(s) 1070
BssT1I CCWWGG 2 cut(s) 267, 498
Bst2BI CACGAG 1 cut(s) 1070
Bst2UI CCWGG 4 cut(s) 375, 440, 542, 944
Bst4CI ACNGT 5 cut(s) 121, 664, 996, 1079, 1096
Bst6I CTCTTC 1 cut(s) 420
BstAFI CTTAAG 1 cut(s) 1204
BstAPI GCANNNNNTGC 1 cut(s) 247
BstAUI TGTACA 1 cut(s) 937
BstC8I GCNNGC 4 cut(s) 172, 674, 1090, 1267
BstDEI CTNAG 3 cut(s) 228, 478, 1364
BstF5I GGATG 2 cut(s) 747, 1393
BstFNI CGCG 1 cut(s) 174
BstH2I RGCGCY 1 cut(s) 1224
BstHHI GCGC 3 cut(s) 325, 876, 1223
BstKTI GATC 4 cut(s) 715, 742, 828, 1383
BstMAI GTCTC 2 cut(s) 531, 1046
BstMBI GATC 4 cut(s) 712, 739, 825, 1380
BstNI CCWGG 4 cut(s) 375, 440, 542, 944
BstNSI RCATGY 1 cut(s) 1028
BstSCI CCNGG 5 cut(s) 48, 373, 438, 540, 942
BstSFI CTRYAG 4 cut(s) 183, 906, 1224, 1326
BstSLI GKGCMC 2 cut(s) 172, 349
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 8 cut(s) 7, 19, 200, 307, 329, 920, 1040, 1210
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 2 cut(s) 739, 825
BstYI RGATCY 2 cut(s) 739, 825
Bsu15I ATCGAT 1 cut(s) 843
BsuRI GGCC 2 cut(s) 365, 438
BsuTUI ATCGAT 1 cut(s) 843
BtsCI GGATG 2 cut(s) 747, 1393
BtsI GCAGTG 1 cut(s) 1233
BtsIMutI CAGTG 2 cut(s) 1158, 1233
Cac8I GCNNGC 4 cut(s) 172, 674, 1090, 1267
CaiI CAGNNNCTG 1 cut(s) 662
CciI TCATGA 1 cut(s) 883
CfoI GCGC 3 cut(s) 325, 876, 1223
Cfr13I GGNCC 3 cut(s) 364, 1019, 1322
ClaI ATCGAT 1 cut(s) 843
CseI GACGC 1 cut(s) 395
Csp6I GTAC 5 cut(s) 117, 938, 1075, 1245, 1299
CviAII CATG 7 cut(s) 421, 815, 833, 884, 1025, 1118, 1139
CviQI GTAC 5 cut(s) 117, 938, 1075, 1245, 1299
DdeI CTNAG 3 cut(s) 228, 478, 1364
DpnI GATC 4 cut(s) 714, 741, 827, 1382
DpnII GATC 4 cut(s) 712, 739, 825, 1380
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
Ecl136II GAGCTC 2 cut(s) 218, 1265
Eco130I CCWWGG 2 cut(s) 267, 498
Eco24I GRGCYC 3 cut(s) 220, 432, 1267
Eco47I GGWCC 2 cut(s) 1019, 1322
Eco47III AGCGCT 1 cut(s) 1222
Eco53kI GAGCTC 2 cut(s) 218, 1265
Eco57I CTGAAG 2 cut(s) 740, 1197
Eco88I CYCGRG 1 cut(s) 1317
EcoICRI GAGCTC 2 cut(s) 218, 1265
EcoO109I RGGNCCY 1 cut(s) 1322
EcoRII CCWGG 4 cut(s) 373, 438, 540, 942
EcoT14I CCWWGG 2 cut(s) 267, 498
EcoT22I ATGCAT 1 cut(s) 1169
EcoT38I GRGCYC 3 cut(s) 220, 432, 1267
ErhI CCWWGG 2 cut(s) 267, 498
FaeI CATG 7 cut(s) 424, 818, 836, 887, 1028, 1121, 1142
FaqI GGGAC 1 cut(s) 480
FatI CATG 7 cut(s) 420, 814, 832, 883, 1024, 1117, 1138
FblI GTMKAC 1 cut(s) 791
Fnu4HI GCNGC 8 cut(s) 21, 33, 189, 318, 321, 909, 1029, 1224
FokI GGATG 2 cut(s) 734, 1400
FriOI GRGCYC 3 cut(s) 220, 432, 1267
Fsp4HI GCNGC 8 cut(s) 21, 33, 189, 318, 321, 909, 1029, 1224
FspBI CTAG 3 cut(s) 63, 204, 753
FspI TGCGCA 1 cut(s) 324
GlaI GCGC 3 cut(s) 324, 875, 1222
GluI GCNGC 8 cut(s) 21, 33, 189, 318, 321, 909, 1029, 1224
HaeII RGCGCY 1 cut(s) 1224
HaeIII GGCC 2 cut(s) 365, 438
HapII CCGG 1 cut(s) 50
HgaI GACGC 1 cut(s) 395
HhaI GCGC 3 cut(s) 325, 876, 1223
Hin1II CATG 7 cut(s) 424, 818, 836, 887, 1028, 1121, 1142
Hin6I GCGC 3 cut(s) 323, 874, 1221
HinP1I GCGC 3 cut(s) 323, 874, 1221
HincII GTYRAC 4 cut(s) 410, 792, 1129, 1216
HindII GTYRAC 4 cut(s) 410, 792, 1129, 1216
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 7 cut(s) 45, 148, 524, 788, 1251, 1309, 1360
HpaII CCGG 1 cut(s) 50
HphI GGTGA 2 cut(s) 634, 1403
Hpy166II GTNNAC 9 cut(s) 147, 170, 410, 792, 940, 1019, 1129, 1216, 1247
Hpy188I TCNGA 5 cut(s) 549, 576, 992, 1051, 1365
Hpy188III TCNNGA 8 cut(s) 131, 204, 230, 528, 696, 710, 884, 1070
Hpy8I GTNNAC 9 cut(s) 147, 170, 410, 792, 940, 1019, 1129, 1216, 1247
Hpy99I CGWCG 2 cut(s) 731, 1262
HpyAV CCTTC 1 cut(s) 1331
HpyCH4III ACNGT 5 cut(s) 121, 664, 996, 1079, 1096
HpyF3I CTNAG 3 cut(s) 228, 478, 1364
Hsp92II CATG 7 cut(s) 424, 818, 836, 887, 1028, 1121, 1142
HspAI GCGC 3 cut(s) 323, 874, 1221
Kzo9I GATC 4 cut(s) 712, 739, 825, 1380
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 1 cut(s) 1262
Lsp1109I GCAGC 8 cut(s) 7, 19, 200, 307, 329, 920, 1040, 1210
LweI GCATC 6 cut(s) 124, 253, 526, 877, 1075, 1111
MaeI CTAG 3 cut(s) 63, 204, 753
MaeIII GTNAC 6 cut(s) 284, 487, 688, 1003, 1154, 1171
MalI GATC 4 cut(s) 714, 741, 827, 1382
MboI GATC 4 cut(s) 712, 739, 825, 1380
MboII GAAGA 5 cut(s) 173, 391, 437, 575, 1190
MflI RGATCY 2 cut(s) 739, 825
MhlI GDGCHC 5 cut(s) 172, 220, 349, 432, 1267
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 5 cut(s) 111, 564, 856, 1101, 1420
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 4 cut(s) 142, 533, 797, 1354
MmeI TCCRAC 2 cut(s) 554, 1015
MnlI CCTC 8 cut(s) 231, 265, 451, 606, 749, 1279, 1313, 1359
Mox20I TGGCCA 1 cut(s) 438
Mph1103I ATGCAT 1 cut(s) 1169
MroXI GAANNNNTTC 1 cut(s) 930
MscI TGGCCA 1 cut(s) 438
MseI TTAA 7 cut(s) 156, 591, 954, 969, 1040, 1205, 1428
MslI CAYNNNNRTG 6 cut(s) 132, 142, 196, 819, 1137, 1143
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 320
MspCI CTTAAG 1 cut(s) 1204
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 5 cut(s) 50, 375, 440, 542, 944
Mva1269I GAATGC 2 cut(s) 203, 1169
MvaI CCWGG 4 cut(s) 375, 440, 542, 944
MvnI CGCG 1 cut(s) 174
NciI CCSGG 1 cut(s) 50
NdeII GATC 4 cut(s) 712, 739, 825, 1380
NlaIII CATG 7 cut(s) 424, 818, 836, 887, 1028, 1121, 1142
NmuCI GTSAC 3 cut(s) 284, 487, 1154
NsbI TGCGCA 1 cut(s) 324
NsiI ATGCAT 1 cut(s) 1169
NspI RCATGY 1 cut(s) 1028
OliI CACNNNNGTG 1 cut(s) 142
PaeR7I CTCGAG 1 cut(s) 1317
PagI TCATGA 1 cut(s) 883
PciSI GCTCTTC 1 cut(s) 420
PctI GAATGC 2 cut(s) 203, 1169
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 3 cut(s) 45, 1251, 1309
PkrI GCNGC 8 cut(s) 22, 34, 190, 319, 322, 910, 1030, 1225
PleI GAGTC 4 cut(s) 142, 532, 796, 1354
PpsI GAGTC 4 cut(s) 142, 532, 796, 1354
PpuMI RGGWCCY 1 cut(s) 1322
Psp124BI GAGCTC 2 cut(s) 220, 1267
Psp5II RGGWCCY 1 cut(s) 1322
Psp6I CCWGG 4 cut(s) 373, 438, 540, 942
PspGI CCWGG 4 cut(s) 373, 438, 540, 942
PspPI GGNCC 3 cut(s) 364, 1019, 1322
PspPPI RGGWCCY 1 cut(s) 1322
PspXI VCTCGAGB 1 cut(s) 1317
PstI CTGCAG 2 cut(s) 910, 1228
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 2 cut(s) 739, 825
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 5 cut(s) 118, 939, 1076, 1246, 1300
RsaNI GTAC 5 cut(s) 117, 938, 1075, 1245, 1299
RseI CAYNNNNRTG 6 cut(s) 132, 142, 196, 819, 1137, 1143
SacI GAGCTC 2 cut(s) 220, 1267
SalI GTCGAC 1 cut(s) 790
SapI GCTCTTC 1 cut(s) 420
SaqAI TTAA 7 cut(s) 156, 591, 954, 969, 1040, 1205, 1428
SatI GCNGC 8 cut(s) 21, 33, 189, 318, 321, 909, 1029, 1224
Sau3AI GATC 4 cut(s) 712, 739, 825, 1380
Sau96I GGNCC 3 cut(s) 364, 1019, 1322
SchI GAGTC 4 cut(s) 142, 533, 797, 1354
ScrFI CCNGG 5 cut(s) 50, 375, 440, 542, 944
SduI GDGCHC 5 cut(s) 172, 220, 349, 432, 1267
SfaNI GCATC 6 cut(s) 124, 253, 526, 877, 1075, 1111
SfcI CTRYAG 4 cut(s) 183, 906, 1224, 1326
Sfr274I CTCGAG 1 cut(s) 1317
SinI GGWCC 2 cut(s) 1019, 1322
SlaI CTCGAG 1 cut(s) 1317
SmiMI CAYNNNNRTG 6 cut(s) 132, 142, 196, 819, 1137, 1143
SmlI CTYRAG 2 cut(s) 1204, 1317
SmoI CTYRAG 2 cut(s) 1204, 1317
SpeI ACTAGT 1 cut(s) 62
Sse9I AATT 5 cut(s) 111, 564, 856, 1101, 1420
SsiI CCGC 1 cut(s) 6
SspI AATATT 2 cut(s) 246, 1199
SspMI CTAG 3 cut(s) 63, 204, 753
SstI GAGCTC 2 cut(s) 220, 1267
StyD4I CCNGG 5 cut(s) 48, 373, 438, 540, 942
StyI CCWWGG 2 cut(s) 267, 498
TaaI ACNGT 5 cut(s) 121, 664, 996, 1079, 1096
TaqI TCGA 8 cut(s) 214, 252, 529, 726, 791, 843, 1318, 1404
TasI AATT 5 cut(s) 111, 564, 856, 1101, 1420
TatI WGTACW 4 cut(s) 937, 1074, 1244, 1298
TfiI GAWTC 3 cut(s) 45, 1251, 1309
Tru1I TTAA 7 cut(s) 156, 591, 954, 969, 1040, 1205, 1428
Tru9I TTAA 7 cut(s) 156, 591, 954, 969, 1040, 1205, 1428
TscAI CASTG 2 cut(s) 1165, 1233
TseFI GTSAC 3 cut(s) 284, 487, 1154
TseI GCWGC 8 cut(s) 20, 32, 188, 317, 320, 908, 1028, 1223
Tsp45I GTSAC 3 cut(s) 284, 487, 1154
TspDTI ATGAA 7 cut(s) 116, 294, 318, 546, 872, 1127, 1200
TspGWI ACGGA 1 cut(s) 1275
TspRI CASTG 2 cut(s) 1165, 1233
Vha464I CTTAAG 1 cut(s) 1204
VneI GTGCAC 1 cut(s) 168
VpaK11BI GGWCC 2 cut(s) 1019, 1322
XapI RAATTY 1 cut(s) 1420
XbaI TCTAGA 1 cut(s) 203
XceI RCATGY 1 cut(s) 1028
XhoI CTCGAG 1 cut(s) 1317
XmiI GTMKAC 1 cut(s) 791
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 3 cut(s) 63, 204, 753
Zsp2I ATGCAT 1 cut(s) 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.