Rorug03G0075300

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000993
Physical Location & Seq
Reverse (-)
5893204 .. 5895347
2144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0075300.1

Sequence Viewer

Length: 1047 bp
ATGTCCTTCCCTCTTATTCTTCAACCCAACAATGTCTTCTTCACCCCTAAAACCCTCACCAGCTTCCCCAAAGCCTTGAGCTCAATTTCAATGTCAGACCAACCCGAATCCACAAAACCCACTTCCCAAAATGCCCTCCAAGCCACGCAGAATTACCCTGTGCCCCTCTCCCCGCCTCTCCCATCTGTCTCAAAGAACATAGAGCTGGCGAGAGCCATGTCCGCCTCTTCCAAATCCAGCCTCTTTTCTCTGTCGCGGGACGACGTCGTTTACGAGGACCAGTGGCTCATTGTTGTCAACAAGCCTCAGGGGATTTACTGTGAGACTGTGCTGGAATCGGTCCCTAAGCTTCTCTTTGACTCGGCCGAGTCAGTTGCAGGGACTGAAGCTATAGTGCCAGAGCTCCATCTTGCAAACCGTCTTGATCGGGACACTAGTGGAGTAATGATAATAACCAAGTCACATAAAGTAGCTGCCAAACTTGTTAAGGCATTTACTGATCATAAAGTTTCCAAAACGTACATTGCACGCTGCATTGGCTCAGCTCCGAAATGGGAAAGAATCACTGTTAGATCGGGTCATGGTCGGTCAAAGTTTGGGGCCTGGAGGGTGTATGCTGCTTCAGATGTAGGCCGGACACTTCCAGGTGGGTCAGCAGTCAGAGATATGGAAACGTCATTTGAAATAATATCGATTAATGGACAAGGGAGCTTCAAAGAGCCATCTGAGTTTAGAACAGATGAAGATAATATTGTAGTAGTTGAAGAAAAATCTGTAGTAGATGGAGATGCAAAGAAGGATGAGATTCTGGTGAGAGCACGTCCTCGGAGTGGAAGGACGCACCAAATCCGCTTGCATTGCCAGTATCTTGGAATTTCGATAAGAGGGGATGTTAAGTATGAGGGTGTCTACGAGTGGAAAGGGACAACATATGAAGGCCATGAACTTCATGCAGAAAGCTTGTCTTTGGAGCACCCTATTACTGGTCTTCCTGTATTATTTCGAGCACCCCTACCATATTGGGCCAGCCAGGCGTTGCAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

348

Amino Acids

38.47

Weight (kDa)

7.74

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 96 - 288 3.9e-22 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 909
AccII CGCG 1 cut(s) 256
AciI CCGC 4 cut(s) 173, 222, 256, 850
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 1 cut(s) 873
AcuI CTGAAG 2 cut(s) 405, 606
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 1 cut(s) 521
AfiI CCNNNNNNNGG 2 cut(s) 830, 983
AgsI TTSAA 5 cut(s) 23, 90, 683, 715, 764
AhlI ACTAGT 1 cut(s) 434
AjiI CACGTC 1 cut(s) 821
AjnI CCWGG 3 cut(s) 602, 643, 1029
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
Alw21I GWGCWC 5 cut(s) 83, 405, 820, 975, 1009
Alw26I GTCTC 2 cut(s) 193, 317
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 5 cut(s) 363, 600, 631, 937, 1023
ApeKI GCWGC 4 cut(s) 473, 531, 617, 1039
ApoI RAATTY 1 cut(s) 873
AseI ATTAAT 1 cut(s) 696
AspS9I GGNCC 4 cut(s) 277, 340, 600, 1023
AsuHPI GGTGA 3 cut(s) 34, 49, 823
AvaII GGWCC 2 cut(s) 277, 340
AxyI CCTNAGG 1 cut(s) 306
BaeGI GKGCMC 1 cut(s) 165
BanII GRGCYC 2 cut(s) 83, 405
BbsI GAAGAC 2 cut(s) 28, 980
Bbv12I GWGCWC 5 cut(s) 83, 405, 820, 975, 1009
BbvI GCAGC 3 cut(s) 460, 518, 604
BccI CCATC 4 cut(s) 190, 414, 730, 776
BcgI CGANNNNNNTGC 2 cut(s) 356, 390
BciT130I CCWGG 3 cut(s) 604, 645, 1031
BclI TGATCA 1 cut(s) 499
BcoDI GTCTC 2 cut(s) 193, 317
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 1 cut(s) 435
BfmI CTRYAG 2 cut(s) 390, 774
BglI GCCNNNNNGGC 1 cut(s) 1031
BisI GCNGC 4 cut(s) 474, 532, 618, 1040
BlpI GCTNAGC 1 cut(s) 541
BlsI GCNGC 4 cut(s) 475, 533, 619, 1041
Bme1390I CCNGG 3 cut(s) 604, 645, 1031
Bme18I GGWCC 2 cut(s) 277, 340
BmgBI CACGTC 1 cut(s) 821
BmgT120I GGNCC 4 cut(s) 277, 340, 600, 1023
BmiI GGNNCC 2 cut(s) 342, 601
BmrFI CCNGG 3 cut(s) 604, 645, 1031
BmsI GCATC 1 cut(s) 778
BpiI GAAGAC 2 cut(s) 28, 980
BpmI CTGGAG 1 cut(s) 625
Bpu10I CCTNAGC 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 541
BpuEI CTTGAG 1 cut(s) 97
Bsa29I ATCGAT 1 cut(s) 692
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 824
Bsc4I CCNNNNNNNGG 2 cut(s) 830, 983
Bse1I ACTGG 3 cut(s) 280, 862, 988
Bse21I CCTNAGG 1 cut(s) 306
Bse3DI GCAATG 2 cut(s) 522, 856
BseBI CCWGG 3 cut(s) 604, 645, 1031
BseCI ATCGAT 1 cut(s) 692
BseDI CCNNGG 1 cut(s) 824
BseGI GGATG 2 cut(s) 805, 895
BseLI CCNNNNNNNGG 2 cut(s) 830, 983
BseMI GCAATG 2 cut(s) 522, 856
BseMII CTCAG 3 cut(s) 320, 555, 717
BseNI ACTGG 3 cut(s) 280, 862, 988
BseSI GKGCMC 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 363
BseXI GCAGC 3 cut(s) 460, 518, 604
Bsh1236I CGCG 1 cut(s) 256
Bsh1285I CGRYCG 1 cut(s) 366
BshFI GGCC 5 cut(s) 365, 602, 633, 939, 1025
BshVI ATCGAT 1 cut(s) 692
BsiEI CGRYCG 1 cut(s) 366
BsiHKAI GWGCWC 5 cut(s) 83, 405, 820, 975, 1009
BsiSI CCGG 1 cut(s) 634
BslFI GGGAC 5 cut(s) 272, 326, 394, 443, 937
BslI CCNNNNNNNGG 2 cut(s) 830, 983
BsmAI GTCTC 2 cut(s) 193, 317
BsmFI GGGAC 5 cut(s) 272, 326, 394, 443, 937
BsnI GGCC 5 cut(s) 365, 602, 633, 939, 1025
Bsp1286I GDGCHC 6 cut(s) 83, 165, 405, 820, 975, 1009
Bsp143I GATC 3 cut(s) 424, 499, 572
Bsp1720I GCTNAGC 1 cut(s) 541
BspACI CCGC 4 cut(s) 173, 222, 256, 850
BspANI GGCC 5 cut(s) 365, 602, 633, 939, 1025
BspCNI CTCAG 3 cut(s) 319, 554, 718
BspDI ATCGAT 1 cut(s) 692
BspFNI CGCG 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 342, 601
BsrDI GCAATG 2 cut(s) 522, 856
BsrI ACTGG 3 cut(s) 280, 862, 988
BssECI CCNNGG 1 cut(s) 824
BssMI GATC 3 cut(s) 424, 499, 572
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 3 cut(s) 604, 645, 1031
Bst4CI ACNGT 4 cut(s) 320, 328, 419, 568
Bst6I CTCTTC 1 cut(s) 232
BstACI GRCGYC 1 cut(s) 264
BstC8I GCNNGC 4 cut(s) 207, 529, 854, 1027
BstDEI CTNAG 4 cut(s) 306, 345, 541, 726
BstF5I GGATG 2 cut(s) 805, 895
BstFNI CGCG 1 cut(s) 256
BstKTI GATC 3 cut(s) 427, 502, 575
BstMAI GTCTC 2 cut(s) 193, 317
BstMBI GATC 3 cut(s) 424, 499, 572
BstMCI CGRYCG 1 cut(s) 366
BstMWI GCNNNNNNNGC 5 cut(s) 140, 221, 537, 858, 1031
BstNI CCWGG 3 cut(s) 604, 645, 1031
BstSCI CCNGG 3 cut(s) 602, 643, 1029
BstSFI CTRYAG 2 cut(s) 390, 774
BstSLI GKGCMC 1 cut(s) 165
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 3 cut(s) 460, 518, 604
BstV2I GAAGAC 2 cut(s) 28, 980
BstXI CCANNNNNNTGG 1 cut(s) 869
BstZI CGGCCG 1 cut(s) 363
Bsu15I ATCGAT 1 cut(s) 692
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 5 cut(s) 365, 602, 633, 939, 1025
BsuTUI ATCGAT 1 cut(s) 692
BtrI CACGTC 1 cut(s) 821
BtsCI GGATG 2 cut(s) 805, 895
BtsIMutI CAGTG 2 cut(s) 287, 564
Cac8I GCNNGC 4 cut(s) 207, 529, 854, 1027
CaiI CAGNNNCTG 1 cut(s) 383
Cfr13I GGNCC 4 cut(s) 277, 340, 600, 1023
ClaI ATCGAT 1 cut(s) 692
CseI GACGC 1 cut(s) 847
Csp6I GTAC 1 cut(s) 520
CviAII CATG 4 cut(s) 217, 581, 941, 950
CviQI GTAC 1 cut(s) 520
DdeI CTNAG 4 cut(s) 306, 345, 541, 726
DpnI GATC 3 cut(s) 426, 501, 574
DpnII GATC 3 cut(s) 424, 499, 572
EaeI YGGCCR 1 cut(s) 363
EagI CGGCCG 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 232
EciI GGCGGA 1 cut(s) 211
Ecl136II GAGCTC 2 cut(s) 81, 403
EclXI CGGCCG 1 cut(s) 363
Eco24I GRGCYC 2 cut(s) 83, 405
Eco47I GGWCC 2 cut(s) 277, 340
Eco52I CGGCCG 1 cut(s) 363
Eco53kI GAGCTC 2 cut(s) 81, 403
Eco57I CTGAAG 2 cut(s) 405, 606
Eco81I CCTNAGG 1 cut(s) 306
EcoICRI GAGCTC 2 cut(s) 81, 403
EcoO109I RGGNCCY 1 cut(s) 600
EcoRII CCWGG 3 cut(s) 602, 643, 1029
EcoT38I GRGCYC 2 cut(s) 83, 405
FaeI CATG 4 cut(s) 220, 584, 944, 953
FalI AAGNNNNNCTT 4 cut(s) 338, 370, 949, 981
FaqI GGGAC 5 cut(s) 272, 326, 394, 443, 937
FatI CATG 4 cut(s) 216, 580, 940, 949
FauI CCCGC 2 cut(s) 180, 249
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 1 cut(s) 909
Fnu4HI GCNGC 4 cut(s) 474, 532, 618, 1040
FokI GGATG 2 cut(s) 812, 902
FriOI GRGCYC 2 cut(s) 83, 405
Fsp4HI GCNGC 4 cut(s) 474, 532, 618, 1040
FspBI CTAG 1 cut(s) 435
GluI GCNGC 4 cut(s) 474, 532, 618, 1040
GsuI CTGGAG 1 cut(s) 625
HaeIII GGCC 5 cut(s) 365, 602, 633, 939, 1025
HapII CCGG 1 cut(s) 634
HgaI GACGC 1 cut(s) 847
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 4 cut(s) 220, 584, 944, 953
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HindIII AAGCTT 2 cut(s) 347, 958
HinfI GANTC 6 cut(s) 107, 335, 359, 368, 561, 805
HpaII CCGG 1 cut(s) 634
HphI GGTGA 3 cut(s) 34, 49, 823
Hpy166II GTNNAC 3 cut(s) 271, 298, 910
Hpy188I TCNGA 6 cut(s) 97, 549, 625, 662, 727, 828
Hpy188III TCNNGA 2 cut(s) 422, 428
Hpy8I GTNNAC 3 cut(s) 271, 298, 910
Hpy99I CGWCG 2 cut(s) 266, 269
HpyAV CCTTC 4 cut(s) 16, 790, 828, 929
HpyCH4III ACNGT 4 cut(s) 320, 328, 419, 568
HpyCH4IV ACGT 4 cut(s) 264, 518, 674, 820
HpyCH4V TGCA 8 cut(s) 377, 413, 527, 534, 791, 856, 953, 1039
HpyF10VI GCNNNNNNNGC 5 cut(s) 140, 221, 537, 858, 1031
HpyF3I CTNAG 4 cut(s) 306, 345, 541, 726
HpySE526I ACGT 4 cut(s) 264, 518, 674, 820
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 4 cut(s) 220, 584, 944, 953
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 3 cut(s) 424, 499, 572
LmnI GCTCC 4 cut(s) 408, 550, 708, 970
Lsp1109I GCAGC 3 cut(s) 460, 518, 604
LweI GCATC 1 cut(s) 778
MaeI CTAG 1 cut(s) 435
MaeII ACGT 4 cut(s) 264, 518, 674, 820
MaeIII GTNAC 1 cut(s) 459
MalI GATC 3 cut(s) 426, 501, 574
MboI GATC 3 cut(s) 424, 499, 572
MboII GAAGA 7 cut(s) 11, 28, 31, 219, 755, 776, 980
MhlI GDGCHC 6 cut(s) 83, 165, 405, 820, 975, 1009
MluCI AATT 3 cut(s) 84, 151, 873
MlyI GAGTC 2 cut(s) 353, 377
MseI TTAA 3 cut(s) 486, 696, 894
MspI CCGG 1 cut(s) 634
MspR9I CCNGG 3 cut(s) 604, 645, 1031
MvaI CCWGG 3 cut(s) 604, 645, 1031
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 5 cut(s) 140, 221, 537, 858, 1031
NdeI CATATG 1 cut(s) 931
NdeII GATC 3 cut(s) 424, 499, 572
NlaIII CATG 4 cut(s) 220, 584, 944, 953
NlaIV GGNNCC 2 cut(s) 342, 601
NmeAIII GCCGAG 2 cut(s) 341, 391
NmuCI GTSAC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PfeI GAWTC 4 cut(s) 107, 335, 561, 805
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 4 cut(s) 475, 533, 619, 1041
PleI GAGTC 2 cut(s) 353, 376
PpsI GAGTC 2 cut(s) 353, 376
PshBI ATTAAT 1 cut(s) 696
Psp124BI GAGCTC 2 cut(s) 83, 405
Psp6I CCWGG 3 cut(s) 602, 643, 1029
PspGI CCWGG 3 cut(s) 602, 643, 1029
PspN4I GGNNCC 2 cut(s) 342, 601
PspPI GGNCC 4 cut(s) 277, 340, 600, 1023
PstNI CAGNNNCTG 1 cut(s) 383
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 1 cut(s) 521
RsaNI GTAC 1 cut(s) 520
SacI GAGCTC 2 cut(s) 83, 405
SaqAI TTAA 3 cut(s) 486, 696, 894
SatI GCNGC 4 cut(s) 474, 532, 618, 1040
Sau3AI GATC 3 cut(s) 424, 499, 572
Sau96I GGNCC 4 cut(s) 277, 340, 600, 1023
SchI GAGTC 2 cut(s) 353, 377
ScrFI CCNGG 3 cut(s) 604, 645, 1031
SduI GDGCHC 6 cut(s) 83, 165, 405, 820, 975, 1009
SfaNI GCATC 1 cut(s) 778
SfcI CTRYAG 2 cut(s) 390, 774
SinI GGWCC 2 cut(s) 277, 340
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
SpeI ACTAGT 1 cut(s) 434
Sse9I AATT 3 cut(s) 84, 151, 873
SsiI CCGC 4 cut(s) 173, 222, 256, 850
SspI AATATT 1 cut(s) 751
SspMI CTAG 1 cut(s) 435
SstI GAGCTC 2 cut(s) 83, 405
StyD4I CCNGG 3 cut(s) 602, 643, 1029
TaaI ACNGT 4 cut(s) 320, 328, 419, 568
TaiI ACGT 4 cut(s) 267, 521, 677, 823
TaqI TCGA 3 cut(s) 692, 878, 1003
TaqII GACCGA 2 cut(s) 328, 576
TasI AATT 3 cut(s) 84, 151, 873
TfiI GAWTC 4 cut(s) 107, 335, 561, 805
Tru1I TTAA 3 cut(s) 486, 696, 894
Tru9I TTAA 3 cut(s) 486, 696, 894
TscAI CASTG 2 cut(s) 287, 571
TseFI GTSAC 1 cut(s) 459
TseI GCWGC 4 cut(s) 473, 531, 617, 1039
Tsp45I GTSAC 1 cut(s) 459
TspDTI ATGAA 4 cut(s) 756, 938, 948, 957
TspRI CASTG 2 cut(s) 287, 571
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 277, 340
VspI ATTAAT 1 cut(s) 696
XapI RAATTY 1 cut(s) 873
XmiI GTMKAC 1 cut(s) 909
XspI CTAG 1 cut(s) 435
ZraI GACGTC 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.