MD00G1084200.v1.1
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0001228
Physical Location & Seq
Reverse (-)
16817531 .. 16819379
1849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1084200.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGTTTCCATTTTATTCGGCAGAATCTTGGCATTTGAGCTTCCGTAGAAATTACTATGCTGGGAATCTCATCAAATTGATGATGACCCATTTGCACAAACTCAGAACAGCATCTATTCTCAAATGGGTTTCTTCAAATTTTGATAAAAACCACTTTAGATCGACAAGAACCCCAATTCACCCAATTGGGTATTTCCCCATTGTCCAAAACCCTAGGTTTTACACAACTAAAAGGGATTCTGTGACTCAAAGTTGTGAAAAAGGGGATACTACTTCGACTGCCATTGCTAATGGCAAAAGTGATGCTCAAATTTCTCGTGCTATTAGAAAAGAAGCGGAAAATGCATTGTTCGATTATTTGTATTTTACTAGATACTTACAGTTTGCAGATGCAGAGAACATGAGTAAAAACTCCCCACATTTTCTCAGGAAGCTTGTAAAATGTGCTGGTAATGAAAAGGGATATGGGGGTTCAATTGCTCGTTTCTTGCGTTACCACCCTATTAATGAATTCGAACCTTTCCTTGAGAGCTTGGGGTTGAAACCTTCCGAGTATAGTCCTCTTCTTCCTCGCAATTTGATGTTCTTGATCGACGATGGTTTGTTGCTTCATAATTATGCTGTTTTGTGTAATTATGGGGTTGCTCGTAATAAGATAGGAAAGATTTATAGGGAAGCTACTGAAATCTTTCAATACCGTGTTGGGGTGTTACGATCAAAGCTTGAAGCTTATGAAGAACTAGGTCTCAGCCAGTCTGATTTGATTAAGCTTGTTGTGTTGGAAAAACTGAAGTCTGTTGGATTTGAAACCAGTTGGATTATAGGGAACTTATCGGAAGAAAATTCTTATAATTGGAGCCGGTTGCTTGAAGCTCTAAGTTTTCTGAGTAAGATGGTTTGCAGCGACAAACAGTTGGGTGAATTAATTGGCCAACACCCACATATTTTATTTGAGGGCTCAGGGGAAAAGATGTTTTCAGTTATTGGGTTCTTACTGAAATTTGGATCCACAAAAAGCCAGCTATGCTCAATATTTCTGCAATTTCCACAGATTCCAGTTGTGAAATTTGTTTCAAATTTGAGACAATGCATTTTGTTCCTGAATGAGATTGACATGAAGGTTGCAGAGATTGGAAAGTTGATTCGTTTTCACCCCTTGCTACTGGGTTCATGTGCTTTGAAGAAAACGAACACCTTACTTCTTCACTTGAAAGTTGGAAAGAAGAGCCTGTGTAGATACATACAGGAGAACCCTCAAGAATTGAAGAATTGGGTTTTAGGTAAAAGACTTAGGCCATTAGAGAACCGTAGATCAATAACAGAGAAGACTATGTTCTTGTTAGACATAGGATTTGTAGAGAACTCAAACAAAATGAAAGTAGCGCAAAAGGCATTTCAAGGCAATGCATGGGAGTTTCAGGAAAGATTTGATTGTATTGTGGAAGCTGGTTTGGATCGCGAGGCTGTCTGCAAAATGATTAAAGCGTACCCTCTAATTCTTAGCCAGTCAAAGGCTGTTATTGAAATGAAGATTGATTTTCTTGTAAATCATTTGCGTTATCCGTTATCAACTGTGCTGACCTTCCCAAGATTTCTTACATATGGAATTAAAAGGGTCAAGCGTAGGGTGTTTATGTACAATTGGCTCAAAGATCAAGGAACGGCAGATCCTAGGTATGCTTTGAGCACTGTTGTTTCGTGTTCAGATGCATGTTTTATAAGGAAGTATGTAAATTGCCATCCTAATGGTCCTGAAGTGTGGGAGGATTTGAAGAATAAAATTCATTTGGAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

599

Amino Acids

69.07

Weight (kDa)

9.43

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 292 - 454 3e-14 mTERF
mTERF PF02536 472 - 572 7.1e-16 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 849, 1718
AccII CGCG 1 cut(s) 1458
AciI CCGC 1 cut(s) 335
AclWI GGATC 4 cut(s) 999, 1012, 1461, 1661
AcoI YGGCCR 1 cut(s) 928
AcsI RAATTY 8 cut(s) 136, 309, 509, 841, 998, 1064, 1075, 1779
AcuI CTGAAG 2 cut(s) 809, 1773
AfaI GTAC 2 cut(s) 1487, 1637
AfiI CCNNNNNNNGG 2 cut(s) 703, 1510
AloI GAACNNNNNNTCC 2 cut(s) 1651, 1683
Alw21I GWGCWC 1 cut(s) 1688
Alw26I GTCTC 2 cut(s) 749, 1075
AlwI GGATC 4 cut(s) 999, 1012, 1461, 1661
AoxI GGCC 2 cut(s) 928, 1292
ApeKI GCWGC 1 cut(s) 900
ApoI RAATTY 8 cut(s) 136, 309, 509, 841, 998, 1064, 1075, 1779
ArsI GACNNNNNNTTYG 2 cut(s) 1334, 1366
AseI ATTAAT 2 cut(s) 504, 923
Asp700I GAANNNNTTC 1 cut(s) 687
AspA2I CCTAGG 2 cut(s) 212, 1670
AspLEI GCGC 1 cut(s) 1384
AspS9I GGNCC 1 cut(s) 1748
AsuHPI GGTGA 3 cut(s) 170, 929, 1142
AsuII TTCGAA 1 cut(s) 513
AvaII GGWCC 1 cut(s) 1748
AvrII CCTAGG 2 cut(s) 212, 1670
BalI TGGCCA 1 cut(s) 930
BamHI GGATCC 1 cut(s) 1004
BanII GRGCYC 1 cut(s) 959
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 1331
Bbv12I GWGCWC 1 cut(s) 1688
BbvI GCAGC 1 cut(s) 912
BccI CCATC 3 cut(s) 590, 886, 1746
BceAI ACGGC 1 cut(s) 1677
BciVI GTATCC 1 cut(s) 259
BcoDI GTCTC 2 cut(s) 749, 1075
BfaI CTAG 4 cut(s) 213, 369, 740, 1671
BfuI GTATCC 1 cut(s) 259
BisI GCNGC 1 cut(s) 901
BlnI CCTAGG 2 cut(s) 212, 1670
BlsI GCNGC 1 cut(s) 902
Bme18I GGWCC 1 cut(s) 1748
BmgT120I GGNCC 1 cut(s) 1748
BmiI GGNNCC 2 cut(s) 857, 1006
BmrI ACTGGG 1 cut(s) 1172
BmsI GCATC 4 cut(s) 119, 292, 379, 1696
BmuI ACTGGG 1 cut(s) 1172
BpiI GAAGAC 1 cut(s) 1331
Bpu10I CCTNAGC 1 cut(s) 958
Bpu14I TTCGAA 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 545, 1239
BsaI GGTCTC 1 cut(s) 749
BsaJI CCNNGG 2 cut(s) 212, 1670
Bsc4I CCNNNNNNNGG 2 cut(s) 703, 1510
Bse118I RCCGGY 1 cut(s) 858
Bse1I ACTGG 5 cut(s) 751, 810, 1055, 1167, 1504
Bse3DI GCAATG 2 cut(s) 282, 1408
BseDI CCNNGG 2 cut(s) 212, 1670
BseGI GGATG 1 cut(s) 1738
BseLI CCNNNNNNNGG 2 cut(s) 703, 1510
BseMI GCAATG 2 cut(s) 282, 1408
BseMII CTCAG 5 cut(s) 115, 439, 760, 875, 972
BseNI ACTGG 5 cut(s) 751, 810, 1055, 1167, 1504
BseXI GCAGC 1 cut(s) 912
BseYI CCCAGC 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 1458
BshFI GGCC 2 cut(s) 930, 1294
BsiHKAI GWGCWC 1 cut(s) 1688
BsiSI CCGG 1 cut(s) 859
BslI CCNNNNNNNGG 2 cut(s) 703, 1510
BsmAI GTCTC 2 cut(s) 749, 1075
BsnI GGCC 2 cut(s) 930, 1294
Bso31I GGTCTC 1 cut(s) 749
Bsp119I TTCGAA 1 cut(s) 513
Bsp1286I GDGCHC 2 cut(s) 959, 1688
Bsp1407I TGTACA 1 cut(s) 1635
Bsp143I GATC 8 cut(s) 158, 588, 713, 1004, 1310, 1453, 1651, 1666
Bsp68I TCGCGA 1 cut(s) 1458
BspACI CCGC 1 cut(s) 335
BspANI GGCC 2 cut(s) 930, 1294
BspCNI CTCAG 5 cut(s) 114, 438, 759, 876, 971
BspFNI CGCG 1 cut(s) 1458
BspLI GGNNCC 2 cut(s) 857, 1006
BspPI GGATC 4 cut(s) 999, 1012, 1461, 1661
BspQI GCTCTTC 1 cut(s) 1217
BspT104I TTCGAA 1 cut(s) 513
BspTNI GGTCTC 1 cut(s) 749
BsrDI GCAATG 2 cut(s) 282, 1408
BsrFI RCCGGY 1 cut(s) 858
BsrGI TGTACA 1 cut(s) 1635
BsrI ACTGG 5 cut(s) 751, 810, 1055, 1167, 1504
BssAI RCCGGY 1 cut(s) 858
BssECI CCNNGG 2 cut(s) 212, 1670
BssMI GATC 8 cut(s) 158, 588, 713, 1004, 1310, 1453, 1651, 1666
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 2 cut(s) 212, 1670
Bst2BI CACGAG 1 cut(s) 315
Bst4CI ACNGT 6 cut(s) 381, 698, 912, 1307, 1573, 1690
Bst6I CTCTTC 2 cut(s) 567, 1217
BstAUI TGTACA 1 cut(s) 1635
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 1019
BstDEI CTNAG 8 cut(s) 101, 425, 746, 875, 884, 958, 1289, 1499
BstF5I GGATG 1 cut(s) 1738
BstFNI CGCG 1 cut(s) 1458
BstHHI GCGC 1 cut(s) 1384
BstKTI GATC 8 cut(s) 161, 591, 716, 1007, 1313, 1456, 1654, 1669
BstMAI GTCTC 2 cut(s) 749, 1075
BstMBI GATC 8 cut(s) 158, 588, 713, 1004, 1310, 1453, 1651, 1666
BstMWI GCNNNNNNNGC 3 cut(s) 341, 1023, 1388
BstNSI RCATGY 1 cut(s) 1713
BstUI CGCG 1 cut(s) 1458
BstV1I GCAGC 1 cut(s) 912
BstV2I GAAGAC 1 cut(s) 1331
BstX2I RGATCY 2 cut(s) 1004, 1666
BstXI CCANNNNNNTGG 1 cut(s) 1745
BstYI RGATCY 2 cut(s) 1004, 1666
BsuI GTATCC 1 cut(s) 259
BsuRI GGCC 2 cut(s) 930, 1294
BtsCI GGATG 1 cut(s) 1738
BtsIMutI CAGTG 1 cut(s) 1686
BtuMI TCGCGA 1 cut(s) 1458
Cac8I GCNNGC 1 cut(s) 1019
CfoI GCGC 1 cut(s) 1384
Cfr10I RCCGGY 1 cut(s) 858
Cfr13I GGNCC 1 cut(s) 1748
Csp6I GTAC 2 cut(s) 1486, 1636
CviAII CATG 5 cut(s) 400, 1114, 1170, 1407, 1710
CviQI GTAC 2 cut(s) 1486, 1636
DdeI CTNAG 8 cut(s) 101, 425, 746, 875, 884, 958, 1289, 1499
DpnI GATC 8 cut(s) 160, 590, 715, 1006, 1312, 1455, 1653, 1668
DpnII GATC 8 cut(s) 158, 588, 713, 1004, 1310, 1453, 1651, 1666
EaeI YGGCCR 1 cut(s) 928
Eam1104I CTCTTC 2 cut(s) 567, 1217
EarI CTCTTC 2 cut(s) 567, 1217
Eco130I CCWWGG 2 cut(s) 212, 1670
Eco24I GRGCYC 1 cut(s) 959
Eco31I GGTCTC 1 cut(s) 749
Eco47I GGWCC 1 cut(s) 1748
Eco57I CTGAAG 2 cut(s) 809, 1773
EcoRI GAATTC 1 cut(s) 509
EcoT14I CCWWGG 2 cut(s) 212, 1670
EcoT22I ATGCAT 4 cut(s) 346, 1091, 1408, 1711
EcoT38I GRGCYC 1 cut(s) 959
ErhI CCWWGG 2 cut(s) 212, 1670
FaeI CATG 5 cut(s) 403, 1117, 1173, 1410, 1713
FatI CATG 5 cut(s) 399, 1113, 1169, 1406, 1709
FauNDI CATATG 1 cut(s) 1600
Fnu4HI GCNGC 1 cut(s) 901
FokI GGATG 1 cut(s) 1725
FriOI GRGCYC 1 cut(s) 959
Fsp4HI GCNGC 1 cut(s) 901
FspBI CTAG 4 cut(s) 213, 369, 740, 1671
GlaI GCGC 1 cut(s) 1383
GluI GCNGC 1 cut(s) 901
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 2 cut(s) 930, 1294
HapII CCGG 1 cut(s) 859
HhaI GCGC 1 cut(s) 1384
Hin1II CATG 5 cut(s) 403, 1117, 1173, 1410, 1713
Hin6I GCGC 1 cut(s) 1382
HinP1I GCGC 1 cut(s) 1382
HindIII AAGCTT 4 cut(s) 431, 719, 726, 767
HinfI GANTC 6 cut(s) 23, 64, 236, 244, 1051, 1141
HpaII CCGG 1 cut(s) 859
HphI GGTGA 3 cut(s) 170, 929, 1142
Hpy188I TCNGA 6 cut(s) 104, 550, 757, 835, 885, 1705
Hpy188III TCNNGA 7 cut(s) 427, 586, 1099, 1256, 1418, 1457, 1751
Hpy99I CGWCG 1 cut(s) 596
HpyAV CCTTC 3 cut(s) 555, 1111, 1591
HpyCH4III ACNGT 6 cut(s) 381, 698, 912, 1307, 1573, 1690
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 1023, 1388
HpyF3I CTNAG 8 cut(s) 101, 425, 746, 875, 884, 958, 1289, 1499
Hsp92II CATG 5 cut(s) 403, 1117, 1173, 1410, 1713
HspAI GCGC 1 cut(s) 1382
Kzo9I GATC 8 cut(s) 158, 588, 713, 1004, 1310, 1453, 1651, 1666
LguI GCTCTTC 1 cut(s) 1217
LmnI GCTCC 2 cut(s) 855, 1789
Lsp1109I GCAGC 1 cut(s) 912
LweI GCATC 4 cut(s) 119, 292, 379, 1696
MaeI CTAG 4 cut(s) 213, 369, 740, 1671
MaeIII GTNAC 3 cut(s) 241, 491, 708
MalI GATC 8 cut(s) 160, 590, 715, 1006, 1312, 1455, 1653, 1668
MboI GATC 8 cut(s) 158, 588, 713, 1004, 1310, 1453, 1651, 1666
MfeI CAATTG 3 cut(s) 183, 474, 1639
MflI RGATCY 2 cut(s) 1004, 1666
MhlI GDGCHC 2 cut(s) 959, 1688
MlsI TGGCCA 1 cut(s) 930
MluNI TGGCCA 1 cut(s) 930
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 4 cut(s) 759, 778, 794, 1195
MnlI CCTC 7 cut(s) 570, 579, 946, 1263, 1453, 1500, 1756
Mox20I TGGCCA 1 cut(s) 930
Mph1103I ATGCAT 4 cut(s) 346, 1091, 1408, 1711
MroXI GAANNNNTTC 1 cut(s) 687
MscI TGGCCA 1 cut(s) 930
MseI TTAA 5 cut(s) 504, 765, 923, 1479, 1608
MslI CAYNNNNRTG 2 cut(s) 615, 1743
Msp20I TGGCCA 1 cut(s) 930
MspI CCGG 1 cut(s) 859
MunI CAATTG 3 cut(s) 183, 474, 1639
MvnI CGCG 1 cut(s) 1458
MwoI GCNNNNNNNGC 3 cut(s) 341, 1023, 1388
NdeI CATATG 1 cut(s) 1600
NdeII GATC 8 cut(s) 158, 588, 713, 1004, 1310, 1453, 1651, 1666
NlaIII CATG 5 cut(s) 403, 1117, 1173, 1410, 1713
NlaIV GGNNCC 2 cut(s) 857, 1006
NmuCI GTSAC 1 cut(s) 241
NruI TCGCGA 1 cut(s) 1458
NsiI ATGCAT 4 cut(s) 346, 1091, 1408, 1711
NspI RCATGY 1 cut(s) 1713
NspV TTCGAA 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 1217
PcsI WCGNNNNNNNCGW 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 5 cut(s) 23, 64, 236, 1051, 1141
PkrI GCNGC 1 cut(s) 902
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PshBI ATTAAT 2 cut(s) 504, 923
PsiI TTATAA 2 cut(s) 849, 1718
PspFI CCCAGC 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 857, 1006
PspPI GGNCC 1 cut(s) 1748
PsuI RGATCY 2 cut(s) 1004, 1666
RruI TCGCGA 1 cut(s) 1458
RsaI GTAC 2 cut(s) 1487, 1637
RsaNI GTAC 2 cut(s) 1486, 1636
RseI CAYNNNNRTG 2 cut(s) 615, 1743
SapI GCTCTTC 1 cut(s) 1217
SaqAI TTAA 5 cut(s) 504, 765, 923, 1479, 1608
SatI GCNGC 1 cut(s) 901
Sau3AI GATC 8 cut(s) 158, 588, 713, 1004, 1310, 1453, 1651, 1666
Sau96I GGNCC 1 cut(s) 1748
SchI GAGTC 1 cut(s) 238
SduI GDGCHC 2 cut(s) 959, 1688
SfaNI GCATC 4 cut(s) 119, 292, 379, 1696
SfuI TTCGAA 1 cut(s) 513
SinI GGWCC 1 cut(s) 1748
SmiMI CAYNNNNRTG 2 cut(s) 615, 1743
SmlI CTYRAG 2 cut(s) 524, 1254
SmoI CTYRAG 2 cut(s) 524, 1254
SsiI CCGC 1 cut(s) 335
SspI AATATT 1 cut(s) 1032
SspMI CTAG 4 cut(s) 213, 369, 740, 1671
StyI CCWWGG 2 cut(s) 212, 1670
TaaI ACNGT 6 cut(s) 381, 698, 912, 1307, 1573, 1690
TaqI TCGA 5 cut(s) 161, 275, 351, 513, 591
TatI WGTACW 1 cut(s) 1635
TfiI GAWTC 5 cut(s) 23, 64, 236, 1051, 1141
Tru1I TTAA 5 cut(s) 504, 765, 923, 1479, 1608
Tru9I TTAA 5 cut(s) 504, 765, 923, 1479, 1608
TscAI CASTG 1 cut(s) 1693
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 1 cut(s) 900
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 9 cut(s) 468, 522, 599, 747, 1130, 1158, 1388, 1541, 1772
TspGWI ACGGA 2 cut(s) 32, 1551
TspRI CASTG 1 cut(s) 1693
VpaK11BI GGWCC 1 cut(s) 1748
VspI ATTAAT 2 cut(s) 504, 923
XapI RAATTY 8 cut(s) 136, 309, 509, 841, 998, 1064, 1075, 1779
XceI RCATGY 1 cut(s) 1713
XmaJI CCTAGG 2 cut(s) 212, 1670
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 4 cut(s) 213, 369, 740, 1671
Zsp2I ATGCAT 4 cut(s) 346, 1091, 1408, 1711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.