MD14G1059800.v1.1
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001228
Physical Location & Seq
Forward (+)
6322579 .. 6324381
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1059800.v1.1.491

Sequence Viewer

Length: 1803 bp
ATGACCCATTTGCAGAAACTCAGATTAGCATCTCTTCTCAGCTGGGTTTCTTCGAATTTGTTCAAAAACCGTGTTAGATCATCACCAATCCCATTTCATCCGATTGGGTATGTCTGCAGTGCCCCAATCTTTAGAATGTACGGAACCAATGGAGCTTCTGAAATTGAGGATTCTGAAAATTCGTATAAAGCTTCGAGTGGTAAGGGAAAAAATGCTGGTCGAATTTCTTGTTCCATTCGAAAAGAAGCTGAAGATGCATTGTTGGATTATTTGCACGGTACTAGAGGGTTGCAGTTCACGGATGCAGAGAATATGAGTAAAAACTCGCCTCTGTTTCTGGACAAGCTTCTGAAACAAGTTGACATTGAAAGAGAAATTCCTGCCACAAAGAATAATCAGAAAGAAATTGGGCGAGCAATTGCACGGTTCTTGCGTTACCACCCTATTAACGAATTTGAGCCTTTCTTTGAGAGCTTGGGTTTGAAGCCTTCTGAGTATTCTCCGCTTCTTCCACGCAGTTTGACGTTTTTGACTGATGATAAGTTATTGGTGCACAATTATACTGTACTATGTCATTATGGGATTGCGCGCAGTAAGATAGGAAAGATTTACAAAGAATCGACAGAAGTCTTTCGATATGATTTTGAGGTTTTAACATCGAAACTCAAAGCTTATGAAGAACTAGGTCTTAGCCAATCTAGTTTTGTTAAGTTTATAGTTGCTACTCCTTGTCTTTTAATTGGGGATGTAAATGTTGAATTTGTTAAGGTATTGGAAAAATTACAGAGTTTTGGATTTGAAACCAATTGGATTGAGGGGAATCTGTCTATTGATGATTCTCATAATTTTCGCCGGATGCTTGAAGTTCTAAGTTTGTTTAGTGAGATGGGTTGCAGCAGCGAACAGTTGGGTCAATTGATTGGCCAGCACCCAGATATTTTGTTTGACGGTTCAGGTGAAAGGACGCTTTCATTAATTGGGTTCTTTCTGAAATTTGGATCTACAAAAAGCCACCTATGCTCAATATTTCTGCAATTTCCACAGATTCCAGTTGTGAAATTTGTTTCAAATTTGAGACAATGCATTTTGTTCCTGAATGAAATTGAAATGAAGGTTGCAGATATTGGGAAGTTTGTCCATACCCACACCCTGCTTCTTGGTTCTTGTTCTTTGAAAAAAACCAGCAGCTTACTCACTAACTTGAACACCGGGAAGAAAAGATTGTGTATATACATCCAGGAGAACCCACAAGAACTGAAGAATTGGGTTTTGGGTAAAAGAGTTCAGCCATTTCCGGACTCGGGAGATGACCTTAGATCAAAGACACAAAAGATTAAGTTTTTGTTTGATGTAGGATTTGTAGACAACTCAGACAATATCAAAGAAGCACTGAAAGCATTTCGAGGCAAAGGAGTGGAGCTTCAGGAAAGATTTGATTGTATTGTTAAAGCTGGTTTGAGTCCAGAGGATGTCTGTACAATGATTAAAATAAGCCCTCAAATTCTTAACCAGACAAAGGATATCATCGAAGAGAAGATTGATTTTCTTGTAAATCAATTGGGTTACCCCATATCGACTTTGGTGACCTTCCCAAGATATCTTTCCCATAAGATCGAAAGGGTCCAGCTTAGAATACTGATGTACAATTGGCTCAATGATCATGGAACAGTTGATCCTCGGCTTTGCTTGAGCACTATTGTTTCTTGCTCAGATAAAAGTTTTATGACTTCTTATGTAAATCGTCATCCTAGTGGTCCTCAAGTGTTGGAGGATTTGAAGAATGAAATTCATTCCAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

601

Amino Acids

68.18

Weight (kDa)

8.5

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 290 - 453 4.5e-09 mTERF
mTERF PF02536 473 - 574 8.4e-15 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1362
AccII CGCG 1 cut(s) 589
AccIII TCCGGA 1 cut(s) 1294
AciI CCGC 1 cut(s) 503
AclWI GGATC 2 cut(s) 1006, 1667
AcoI YGGCCR 1 cut(s) 922
AcuI CTGAAG 3 cut(s) 270, 1277, 1406
AfaI GTAC 5 cut(s) 140, 280, 567, 1477, 1643
AfiI CCNNNNNNNGG 2 cut(s) 1301, 1516
AjnI CCWGG 1 cut(s) 1236
AjuI GAANNNNNNNTTGG 4 cut(s) 1253, 1285, 1585, 1617
AloI GAACNNNNNNTCC 2 cut(s) 1657, 1689
Alw21I GWGCWC 2 cut(s) 555, 1694
Alw26I GTCTC 1 cut(s) 1069
Alw44I GTGCAC 1 cut(s) 551
AlwI GGATC 2 cut(s) 1006, 1667
Ama87I CYCGRG 1 cut(s) 1300
Aor13HI TCCGGA 1 cut(s) 1294
AoxI GGCC 1 cut(s) 922
ApaLI GTGCAC 1 cut(s) 551
ApeKI GCWGC 3 cut(s) 894, 897, 1185
AseI ATTAAT 1 cut(s) 974
Asp700I GAANNNNTTC 2 cut(s) 59, 630
AspLEI GCGC 2 cut(s) 589, 591
AspS9I GGNCC 2 cut(s) 1621, 1754
AsuC2I CCSGG 1 cut(s) 1210
AsuHPI GGTGA 3 cut(s) 75, 968, 1594
AsuII TTCGAA 2 cut(s) 53, 238
AvaI CYCGRG 1 cut(s) 1300
AvaII GGWCC 2 cut(s) 1621, 1754
BaeGI GKGCMC 2 cut(s) 124, 555
BalI TGGCCA 1 cut(s) 924
Bbv12I GWGCWC 2 cut(s) 555, 1694
BbvI GCAGC 3 cut(s) 906, 909, 1197
BccI CCATC 1 cut(s) 880
BciT130I CCWGG 1 cut(s) 1238
BclI TGATCA 1 cut(s) 1657
BcnI CCSGG 1 cut(s) 1210
BcoDI GTCTC 1 cut(s) 1069
BfaI CTAG 4 cut(s) 282, 683, 699, 1749
BfmI CTRYAG 1 cut(s) 115
BisI GCNGC 3 cut(s) 895, 898, 1186
BlsI GCNGC 3 cut(s) 896, 899, 1187
Bme1390I CCNGG 2 cut(s) 1210, 1238
Bme18I GGWCC 2 cut(s) 1621, 1754
BmeT110I CYCGRG 1 cut(s) 1300
BmgT120I GGNCC 2 cut(s) 1621, 1754
BmiI GGNNCC 2 cut(s) 145, 1622
BmrFI CCNGG 2 cut(s) 1210, 1238
BmsI GCATC 4 cut(s) 38, 244, 292, 846
BoxI GACNNNNGTC 1 cut(s) 626
Bpu14I TTCGAA 2 cut(s) 53, 238
BpuEI CTTGAG 2 cut(s) 1708, 1743
BpuMI CCSGG 1 cut(s) 1210
BsaBI GATNNNNATC 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 1676
BsaWI WCCGGW 1 cut(s) 1294
Bsc4I CCNNNNNNNGG 2 cut(s) 1301, 1516
Bse1I ACTGG 1 cut(s) 1049
Bse8I GATNNNNATC 1 cut(s) 28
BseAI TCCGGA 1 cut(s) 1294
BseBI CCWGG 1 cut(s) 1238
BseDI CCNNGG 1 cut(s) 1676
BseGI GGATG 7 cut(s) 97, 307, 751, 861, 1233, 1474, 1744
BseJI GATNNNNATC 1 cut(s) 28
BseLI CCNNNNNNNGG 2 cut(s) 1301, 1516
BseMII CTCAG 5 cut(s) 34, 52, 483, 1383, 1722
BseNI ACTGG 1 cut(s) 1049
BsePI GCGCGC 1 cut(s) 587
BseSI GKGCMC 2 cut(s) 124, 555
BseXI GCAGC 3 cut(s) 906, 909, 1197
BseYI CCCAGC 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 589
BshFI GGCC 1 cut(s) 924
BsiHKAI GWGCWC 2 cut(s) 555, 1694
BsiHKCI CYCGRG 1 cut(s) 1300
BsiSI CCGG 3 cut(s) 853, 1209, 1295
BslI CCNNNNNNNGG 2 cut(s) 1301, 1516
BsmAI GTCTC 1 cut(s) 1069
BsnI GGCC 1 cut(s) 924
BsoBI CYCGRG 1 cut(s) 1300
Bsp119I TTCGAA 2 cut(s) 53, 238
Bsp1286I GDGCHC 3 cut(s) 124, 555, 1694
Bsp13I TCCGGA 1 cut(s) 1294
Bsp1407I TGTACA 2 cut(s) 1475, 1641
Bsp143I GATC 6 cut(s) 77, 998, 1316, 1611, 1657, 1672
BspACI CCGC 1 cut(s) 503
BspANI GGCC 1 cut(s) 924
BspCNI CTCAG 5 cut(s) 33, 51, 484, 1382, 1721
BspEI TCCGGA 1 cut(s) 1294
BspFNI CGCG 1 cut(s) 589
BspLI GGNNCC 2 cut(s) 145, 1622
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 2 cut(s) 1006, 1667
BspT104I TTCGAA 2 cut(s) 53, 238
BsrGI TGTACA 2 cut(s) 1475, 1641
BsrI ACTGG 1 cut(s) 1049
BssECI CCNNGG 1 cut(s) 1676
BssHII GCGCGC 1 cut(s) 587
BssMI GATC 6 cut(s) 77, 998, 1316, 1611, 1657, 1672
Bst2UI CCWGG 1 cut(s) 1238
Bst4CI ACNGT 7 cut(s) 71, 278, 426, 565, 906, 950, 1669
Bst6I CTCTTC 2 cut(s) 39, 1524
BstAUI TGTACA 2 cut(s) 1475, 1641
BstBI TTCGAA 2 cut(s) 53, 238
BstC8I GCNNGC 3 cut(s) 414, 589, 926
BstDEI CTNAG 9 cut(s) 20, 38, 492, 689, 869, 1313, 1369, 1628, 1708
BstEII GGTNACC 2 cut(s) 1562, 1582
BstF5I GGATG 7 cut(s) 97, 307, 751, 861, 1233, 1474, 1744
BstFNI CGCG 1 cut(s) 589
BstHHI GCGC 2 cut(s) 589, 591
BstKTI GATC 6 cut(s) 80, 1001, 1319, 1614, 1660, 1675
BstMAI GTCTC 1 cut(s) 1069
BstMBI GATC 6 cut(s) 77, 998, 1316, 1611, 1657, 1672
BstMWI GCNNNNNNNGC 3 cut(s) 254, 1017, 1394
BstNI CCWGG 1 cut(s) 1238
BstPAI GACNNNNGTC 1 cut(s) 626
BstPI GGTNACC 2 cut(s) 1562, 1582
BstSCI CCNGG 2 cut(s) 1208, 1236
BstSFI CTRYAG 1 cut(s) 115
BstSLI GKGCMC 2 cut(s) 124, 555
BstUI CGCG 1 cut(s) 589
BstV1I GCAGC 3 cut(s) 906, 909, 1197
BstX2I RGATCY 1 cut(s) 998
BstYI RGATCY 1 cut(s) 998
BsuRI GGCC 1 cut(s) 924
BtsCI GGATG 7 cut(s) 97, 307, 751, 861, 1233, 1474, 1744
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 2 cut(s) 124, 1388
Cac8I GCNNGC 3 cut(s) 414, 589, 926
CfoI GCGC 2 cut(s) 589, 591
Cfr13I GGNCC 2 cut(s) 1621, 1754
CseI GACGC 1 cut(s) 973
Csp6I GTAC 5 cut(s) 139, 279, 566, 1476, 1642
CspCI CAANNNNNGTGG 2 cut(s) 501, 536
CviAII CATG 1 cut(s) 1661
CviQI GTAC 5 cut(s) 139, 279, 566, 1476, 1642
DdeI CTNAG 9 cut(s) 20, 38, 492, 689, 869, 1313, 1369, 1628, 1708
DpnI GATC 6 cut(s) 79, 1000, 1318, 1613, 1659, 1674
DpnII GATC 6 cut(s) 77, 998, 1316, 1611, 1657, 1672
EaeI YGGCCR 1 cut(s) 922
Eam1104I CTCTTC 2 cut(s) 39, 1524
EarI CTCTTC 2 cut(s) 39, 1524
Eco32I GATATC 2 cut(s) 1522, 1598
Eco47I GGWCC 2 cut(s) 1621, 1754
Eco57I CTGAAG 3 cut(s) 270, 1277, 1406
Eco88I CYCGRG 1 cut(s) 1300
Eco91I GGTNACC 2 cut(s) 1562, 1582
EcoO65I GGTNACC 2 cut(s) 1562, 1582
EcoRII CCWGG 1 cut(s) 1236
EcoRV GATATC 2 cut(s) 1522, 1598
EcoT22I ATGCAT 2 cut(s) 259, 1085
FaeI CATG 1 cut(s) 1664
FatI CATG 1 cut(s) 1660
FbaI TGATCA 1 cut(s) 1657
FblI GTMKAC 1 cut(s) 1362
Fnu4HI GCNGC 3 cut(s) 895, 898, 1186
FokI GGATG 7 cut(s) 84, 314, 758, 868, 1220, 1481, 1731
Fsp4HI GCNGC 3 cut(s) 895, 898, 1186
FspBI CTAG 4 cut(s) 282, 683, 699, 1749
GlaI GCGC 2 cut(s) 588, 590
GluI GCNGC 3 cut(s) 895, 898, 1186
GsaI CCCAGC 1 cut(s) 46
HaeIII GGCC 1 cut(s) 924
HapII CCGG 3 cut(s) 853, 1209, 1295
HgaI GACGC 1 cut(s) 973
HhaI GCGC 2 cut(s) 589, 591
Hin1II CATG 1 cut(s) 1664
Hin6I GCGC 2 cut(s) 587, 589
HinP1I GCGC 2 cut(s) 587, 589
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 3 cut(s) 189, 344, 669
HinfI GANTC 7 cut(s) 170, 617, 820, 836, 1045, 1298, 1459
HpaII CCGG 3 cut(s) 853, 1209, 1295
HphI GGTGA 3 cut(s) 75, 968, 1594
Hpy166II GTNNAC 4 cut(s) 297, 361, 553, 1363
Hpy188III TCNNGA 6 cut(s) 338, 1093, 1295, 1302, 1424, 1463
Hpy8I GTNNAC 4 cut(s) 297, 361, 553, 1363
HpyAV CCTTC 3 cut(s) 498, 1105, 1597
HpyCH4III ACNGT 7 cut(s) 71, 278, 426, 565, 906, 950, 1669
HpyCH4IV ACGT 1 cut(s) 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 1017, 1394
HpyF3I CTNAG 9 cut(s) 20, 38, 492, 689, 869, 1313, 1369, 1628, 1708
HpySE526I ACGT 1 cut(s) 524
Hsp92II CATG 1 cut(s) 1664
HspAI GCGC 2 cut(s) 587, 589
Kpn2I TCCGGA 1 cut(s) 1294
Ksp22I TGATCA 1 cut(s) 1657
Kzo9I GATC 6 cut(s) 77, 998, 1316, 1611, 1657, 1672
LmnI GCTCC 2 cut(s) 152, 1417
Lsp1109I GCAGC 3 cut(s) 906, 909, 1197
LweI GCATC 4 cut(s) 38, 244, 292, 846
MaeI CTAG 4 cut(s) 282, 683, 699, 1749
MaeII ACGT 1 cut(s) 524
MaeIII GTNAC 3 cut(s) 434, 1562, 1582
MalI GATC 6 cut(s) 79, 1000, 1318, 1613, 1659, 1674
MboI GATC 6 cut(s) 77, 998, 1316, 1611, 1657, 1672
MfeI CAATTG 5 cut(s) 417, 805, 914, 1556, 1645
MflI RGATCY 1 cut(s) 998
MhlI GDGCHC 3 cut(s) 124, 555, 1694
MlsI TGGCCA 1 cut(s) 924
MluNI TGGCCA 1 cut(s) 924
MlyI GAGTC 2 cut(s) 1292, 1468
MmeI TCCRAC 2 cut(s) 243, 1746
Mox20I TGGCCA 1 cut(s) 924
Mph1103I ATGCAT 2 cut(s) 259, 1085
MroI TCCGGA 1 cut(s) 1294
MroXI GAANNNNTTC 2 cut(s) 59, 630
MscI TGGCCA 1 cut(s) 924
MslI CAYNNNNRTG 1 cut(s) 1749
Msp20I TGGCCA 1 cut(s) 924
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 3 cut(s) 853, 1209, 1295
MspR9I CCNGG 2 cut(s) 1210, 1238
MunI CAATTG 5 cut(s) 417, 805, 914, 1556, 1645
MvaI CCWGG 1 cut(s) 1238
MvnI CGCG 1 cut(s) 589
MwoI GCNNNNNNNGC 3 cut(s) 254, 1017, 1394
NciI CCSGG 1 cut(s) 1210
NdeII GATC 6 cut(s) 77, 998, 1316, 1611, 1657, 1672
NlaIII CATG 1 cut(s) 1664
NlaIV GGNNCC 2 cut(s) 145, 1622
NmeAIII GCCGAG 1 cut(s) 1657
NmuCI GTSAC 1 cut(s) 1582
NsiI ATGCAT 2 cut(s) 259, 1085
NspV TTCGAA 2 cut(s) 53, 238
PauI GCGCGC 1 cut(s) 587
PcsI WCGNNNNNNNCGW 1 cut(s) 430
PdmI GAANNNNTTC 2 cut(s) 59, 630
PfeI GAWTC 5 cut(s) 170, 617, 820, 836, 1045
PfoI TCCNGGA 1 cut(s) 1236
PkrI GCNGC 3 cut(s) 896, 899, 1187
PleI GAGTC 2 cut(s) 1292, 1467
PpsI GAGTC 2 cut(s) 1292, 1467
PshAI GACNNNNGTC 1 cut(s) 626
PshBI ATTAAT 1 cut(s) 974
Psp6I CCWGG 1 cut(s) 1236
PspEI GGTNACC 2 cut(s) 1562, 1582
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 1 cut(s) 1236
PspN4I GGNNCC 2 cut(s) 145, 1622
PspPI GGNCC 2 cut(s) 1621, 1754
PstI CTGCAG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 998
PteI GCGCGC 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 42
RsaI GTAC 5 cut(s) 140, 280, 567, 1477, 1643
RsaNI GTAC 5 cut(s) 139, 279, 566, 1476, 1642
RseI CAYNNNNRTG 1 cut(s) 1749
SatI GCNGC 3 cut(s) 895, 898, 1186
Sau3AI GATC 6 cut(s) 77, 998, 1316, 1611, 1657, 1672
Sau96I GGNCC 2 cut(s) 1621, 1754
SchI GAGTC 2 cut(s) 1292, 1468
ScrFI CCNGG 2 cut(s) 1210, 1238
SduI GDGCHC 3 cut(s) 124, 555, 1694
SfaNI GCATC 4 cut(s) 38, 244, 292, 846
SfcI CTRYAG 1 cut(s) 115
SfuI TTCGAA 2 cut(s) 53, 238
SinI GGWCC 2 cut(s) 1621, 1754
SmiMI CAYNNNNRTG 1 cut(s) 1749
SmlI CTYRAG 2 cut(s) 1687, 1758
SmoI CTYRAG 2 cut(s) 1687, 1758
SsiI CCGC 1 cut(s) 503
SspI AATATT 1 cut(s) 1026
SspMI CTAG 4 cut(s) 282, 683, 699, 1749
StyD4I CCNGG 2 cut(s) 1208, 1236
TaaI ACNGT 7 cut(s) 71, 278, 426, 565, 906, 950, 1669
TaiI ACGT 1 cut(s) 527
TatI WGTACW 3 cut(s) 565, 1475, 1641
TfiI GAWTC 5 cut(s) 170, 617, 820, 836, 1045
TscAI CASTG 2 cut(s) 124, 1395
TseFI GTSAC 1 cut(s) 1582
TseI GCWGC 3 cut(s) 894, 897, 1185
Tsp45I GTSAC 1 cut(s) 1582
TspDTI ATGAA 7 cut(s) 86, 690, 960, 1113, 1124, 1778, 1797
TspGWI ACGGA 2 cut(s) 156, 314
TspRI CASTG 2 cut(s) 124, 1395
VneI GTGCAC 1 cut(s) 551
VpaK11BI GGWCC 2 cut(s) 1621, 1754
VspI ATTAAT 1 cut(s) 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 1576
XmiI GTMKAC 1 cut(s) 1362
XmnI GAANNNNTTC 2 cut(s) 59, 630
XspI CTAG 4 cut(s) 282, 683, 699, 1749
Zsp2I ATGCAT 2 cut(s) 259, 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.