MD12G1058200.v1.1
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001228
Physical Location & Seq
Reverse (-)
6676345 .. 6678147
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1058200.v1.1.491

Sequence Viewer

Length: 1803 bp
ATGACCCATTTACAGAAACTCAGATTAGCATCTGTTCGCAAATGGGTTTCGTTGAATTGTTGCGAAAACCGTGTTAGATCACGTCCAATCCCATTTCAGCCATTTGGGTATTTCTGCAATGCCCCAACCTTTAGAACGTACAGTACCAATGGAGCTTCTGCAAATGAGAGTTCCGAAAGTTGGGATAAAACTTCGAGTGGTAAGGGAAGAAATGCTGTTCGAATTTCTTATTCCATTCGAAAAGAAGCGGAAGCTGCATTGTTGGATTATTTGCATGGTACTAGAGGGTTGCAGTTCACGGATGCAGAGAATATGAGTAAAAACTCGCCTTGCTTTCTCGACAAGCTTCTGAGACAGGTTGATGTTGAAAAAGAAATTCTTGCAAAGAAGAATAATCAGAAAGATATTGGGCGAGCAATTGCGCGGTTCTTGCGGTACCATCCTATTAACGAATTTGAGCCTTTCTTTGAGAGCTTGGGTCTGAATCCTTCTGAGTATTCTCTGCTTCTTCCACGCAGTTTGACGTTTTTGACTGATGATAAGTTATTAGCGCACAATTATACTGTACTATGTCATTATGGAATTGCACGCAGTAAGATAGGAAAGATTTACAAGGAATGGACAGAAGTCTTTCAATATGACTTTGAGGTTTTAACATCGAAACTTAAAGCTTATGAAGAACTAGGTCTTAGCCAATCTACTTTTGTTAAGTTTATAGTTGCTACTCCTTGTCTTTTAATTGGGGATGTAAATGTTGAATTTGTTAAGGTTTTGGAAAAATTGCAGAATTTTGGATTTAAAACCAATTGGATTGAAGATAAATTGTCAGCAGATAGTTCTCATAATTGGAGCCGGATGCTTGAAGTTCTTAGTTTGTTTAGTGAGATGGGTTGCAGTACCGAACAGTTGGGTCAATTGATTGGCCAGCACCCAGATATTTTGTTTGACGGTTCTGGTGAAAGGACACTTTCACTAATTGGGTTCTTATTGAAATTTGGATCCACAAAAAGCCAGCTGTGTTCAATATTTCTGCAATTTCCACAGATTCCAGTTGTGAAATTTGTTTCAAATTTAAGACGATGCATTTTGTTCCTGAATGAGATTGACATGAATGTTGCAGAGATTGGGAAGATTGTCCATTCCCACCCCCTGCTTCTCGGTTCTTGTTCTTTGAAAAAAACCATCAGTTTACTCTCAAACTTGAACACTGGGAAGAAAAGATTGTGTACATGCATCCAGGAGAACCCACAAGTATTGAAGAATTGGGTTTTGGGTAAAAGAGTTGAGCGAATTCCAGATTCGGGAGATGACCTTAGATCAAAGACACAAAAGATTAAGTTTTTGTTGGATGTAGGATTTGTAGACAACTCAGACAATATTAAAGAAGCACTGAAAGCATTTCGAGGCAAGGGAGTGGAGCTTCAGGAAAGATTTGATTGTATTGTTAAAGCTGGTTTGAGTCCAGAGGATGTCTGTACAATGATTAAAATAAGCCCTCAAATTCTTAACCAGACGAAGGATATCATTGAAGAGAAGATTGATTTTCTTGTAAATCAATTGGGTTACCCCATATCGACCTTGGTGACCTTCCCAAGATATCTTTCCTATAAGATTGAAAGGGTCCAGCTTAGGACATTGATGTACAATTGGCTCAAAGATCAAGGAACAGCTGATCCTCGGCTTAACTTTAGCACTATTATTTGTTGCTCAGATAAATGTTTTATTACTTCTTATGTAAATCGTCATCCCAGTGGTCCTCAAATGTGGGAGGATTTGAAGAATGAAATTTATTCCAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

601

Amino Acids

68.52

Weight (kDa)

8.84

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 290 - 454 2.2e-13 mTERF
mTERF PF02536 474 - 574 7e-15 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 435
AccI GTMKAC 1 cut(s) 1362
AccII CGCG 1 cut(s) 424
AciI CCGC 3 cut(s) 248, 424, 433
AclWI GGATC 3 cut(s) 993, 1006, 1667
AcoI YGGCCR 1 cut(s) 922
AcuI CTGAAG 1 cut(s) 1406
AfaI GTAC 9 cut(s) 140, 145, 280, 437, 567, 898, 1228, 1477, 1643
AfiI CCNNNNNNNGG 3 cut(s) 180, 1301, 1516
AjiI CACGTC 1 cut(s) 83
AjnI CCWGG 1 cut(s) 1236
AjuI GAANNNNNNNTTGG 4 cut(s) 1253, 1285, 1585, 1617
AloI GAACNNNNNNTCC 2 cut(s) 1657, 1689
Alw26I GTCTC 1 cut(s) 346
AlwI GGATC 3 cut(s) 993, 1006, 1667
AoxI GGCC 1 cut(s) 922
ApeKI GCWGC 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 630
Asp718I GGTACC 1 cut(s) 435
AspLEI GCGC 2 cut(s) 424, 553
AspS9I GGNCC 2 cut(s) 1621, 1754
AsuHPI GGTGA 2 cut(s) 968, 1594
AsuII TTCGAA 2 cut(s) 220, 238
AvaII GGWCC 2 cut(s) 1621, 1754
BalI TGGCCA 1 cut(s) 924
BamHI GGATCC 1 cut(s) 998
BanI GGYRCC 1 cut(s) 435
BbvI GCAGC 1 cut(s) 241
BccI CCATC 3 cut(s) 447, 880, 1190
BciT130I CCWGG 1 cut(s) 1238
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 2 cut(s) 282, 683
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 1 cut(s) 1238
Bme18I GGWCC 2 cut(s) 1621, 1754
BmgBI CACGTC 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 1621, 1754
BmiI GGNNCC 4 cut(s) 437, 851, 1000, 1622
BmrFI CCNGG 1 cut(s) 1238
BmrI ACTGGG 2 cut(s) 1218, 1743
BmsI GCATC 5 cut(s) 38, 292, 846, 1070, 1242
BmuI ACTGGG 2 cut(s) 1218, 1743
BoxI GACNNNNGTC 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 1628
Bpu14I TTCGAA 2 cut(s) 220, 238
BsaBI GATNNNNATC 1 cut(s) 28
BsaJI CCNNGG 2 cut(s) 1578, 1676
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 1301, 1516
Bse1I ACTGG 3 cut(s) 1049, 1213, 1749
Bse3DI GCAATG 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 28
BseBI CCWGG 1 cut(s) 1238
BseDI CCNNGG 2 cut(s) 1578, 1676
BseGI GGATG 8 cut(s) 307, 439, 751, 861, 1233, 1354, 1474, 1744
BseJI GATNNNNATC 1 cut(s) 28
BseLI CCNNNNNNNGG 3 cut(s) 180, 1301, 1516
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 5 cut(s) 34, 341, 483, 1383, 1722
BseNI ACTGG 3 cut(s) 1049, 1213, 1749
BseXI GCAGC 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 424
BshFI GGCC 1 cut(s) 924
BshNI GGYRCC 1 cut(s) 435
BsiSI CCGG 1 cut(s) 853
BslI CCNNNNNNNGG 3 cut(s) 180, 1301, 1516
BsmAI GTCTC 1 cut(s) 346
BsnI GGCC 1 cut(s) 924
Bsp119I TTCGAA 2 cut(s) 220, 238
Bsp1407I TGTACA 3 cut(s) 1226, 1475, 1641
Bsp143I GATC 5 cut(s) 77, 998, 1316, 1657, 1672
BspACI CCGC 3 cut(s) 248, 424, 433
BspANI GGCC 1 cut(s) 924
BspCNI CTCAG 5 cut(s) 33, 342, 484, 1382, 1721
BspFNI CGCG 1 cut(s) 424
BspLI GGNNCC 4 cut(s) 437, 851, 1000, 1622
BspPI GGATC 3 cut(s) 993, 1006, 1667
BspT104I TTCGAA 2 cut(s) 220, 238
BspT107I GGYRCC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 124
BsrGI TGTACA 3 cut(s) 1226, 1475, 1641
BsrI ACTGG 3 cut(s) 1049, 1213, 1749
BssECI CCNNGG 2 cut(s) 1578, 1676
BssMI GATC 5 cut(s) 77, 998, 1316, 1657, 1672
BssT1I CCWWGG 1 cut(s) 1578
Bst2UI CCWGG 1 cut(s) 1238
Bst4CI ACNGT 5 cut(s) 71, 143, 565, 906, 950
Bst6I CTCTTC 1 cut(s) 1524
BstAUI TGTACA 3 cut(s) 1226, 1475, 1641
BstBI TTCGAA 2 cut(s) 220, 238
BstC8I GCNNGC 4 cut(s) 414, 589, 926, 1013
BstDEI CTNAG 9 cut(s) 20, 350, 492, 689, 869, 1313, 1369, 1628, 1708
BstEII GGTNACC 2 cut(s) 1562, 1582
BstF5I GGATG 8 cut(s) 307, 439, 751, 861, 1233, 1354, 1474, 1744
BstFNI CGCG 1 cut(s) 424
BstHHI GCGC 2 cut(s) 424, 553
BstKTI GATC 5 cut(s) 80, 1001, 1319, 1660, 1675
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 5 cut(s) 77, 998, 1316, 1657, 1672
BstMWI GCNNNNNNNGC 3 cut(s) 254, 430, 1394
BstNI CCWGG 1 cut(s) 1238
BstNSI RCATGY 1 cut(s) 1233
BstPAI GACNNNNGTC 1 cut(s) 626
BstPI GGTNACC 2 cut(s) 1562, 1582
BstSCI CCNGG 1 cut(s) 1236
BstUI CGCG 1 cut(s) 424
BstV1I GCAGC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 998
BstYI RGATCY 1 cut(s) 998
BsuRI GGCC 1 cut(s) 924
BtrI CACGTC 1 cut(s) 83
BtsCI GGATG 8 cut(s) 307, 439, 751, 861, 1233, 1354, 1474, 1744
BtsIMutI CAGTG 3 cut(s) 1206, 1388, 1756
Cac8I GCNNGC 4 cut(s) 414, 589, 926, 1013
CfoI GCGC 2 cut(s) 424, 553
Cfr13I GGNCC 2 cut(s) 1621, 1754
Csp6I GTAC 9 cut(s) 139, 144, 279, 436, 566, 897, 1227, 1476, 1642
CspCI CAANNNNNGTGG 4 cut(s) 501, 536, 1236, 1271
CviAII CATG 3 cut(s) 275, 1108, 1230
CviQI GTAC 9 cut(s) 139, 144, 279, 436, 566, 897, 1227, 1476, 1642
DdeI CTNAG 9 cut(s) 20, 350, 492, 689, 869, 1313, 1369, 1628, 1708
DpnI GATC 5 cut(s) 79, 1000, 1318, 1659, 1674
DpnII GATC 5 cut(s) 77, 998, 1316, 1657, 1672
DraI TTTAAA 1 cut(s) 799
EaeI YGGCCR 1 cut(s) 922
Eam1104I CTCTTC 1 cut(s) 1524
EarI CTCTTC 1 cut(s) 1524
Eco130I CCWWGG 1 cut(s) 1578
Eco32I GATATC 2 cut(s) 1522, 1598
Eco47I GGWCC 2 cut(s) 1621, 1754
Eco57I CTGAAG 1 cut(s) 1406
Eco91I GGTNACC 2 cut(s) 1562, 1582
EcoO65I GGTNACC 2 cut(s) 1562, 1582
EcoRI GAATTC 1 cut(s) 1290
EcoRII CCWGG 1 cut(s) 1236
EcoRV GATATC 2 cut(s) 1522, 1598
EcoT14I CCWWGG 1 cut(s) 1578
EcoT22I ATGCAT 2 cut(s) 1085, 1235
ErhI CCWWGG 1 cut(s) 1578
FaeI CATG 3 cut(s) 278, 1111, 1233
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FatI CATG 3 cut(s) 274, 1107, 1229
FblI GTMKAC 1 cut(s) 1362
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 8 cut(s) 314, 426, 758, 868, 1220, 1361, 1481, 1731
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 2 cut(s) 282, 683
GlaI GCGC 2 cut(s) 423, 552
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 1 cut(s) 924
HapII CCGG 1 cut(s) 853
HhaI GCGC 2 cut(s) 424, 553
Hin1II CATG 3 cut(s) 278, 1111, 1233
Hin6I GCGC 2 cut(s) 422, 551
HinP1I GCGC 2 cut(s) 422, 551
HindIII AAGCTT 2 cut(s) 344, 669
HinfI GANTC 4 cut(s) 484, 1045, 1298, 1459
HpaII CCGG 1 cut(s) 853
HphI GGTGA 2 cut(s) 968, 1594
Hpy166II GTNNAC 4 cut(s) 297, 1190, 1227, 1363
Hpy188I TCNGA 8 cut(s) 23, 175, 351, 399, 483, 493, 1372, 1711
Hpy188III TCNNGA 6 cut(s) 338, 1093, 1295, 1302, 1424, 1463
Hpy8I GTNNAC 4 cut(s) 297, 1190, 1227, 1363
HpyAV CCTTC 3 cut(s) 498, 1510, 1597
HpyCH4III ACNGT 5 cut(s) 71, 143, 565, 906, 950
HpyCH4IV ACGT 3 cut(s) 82, 137, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 430, 1394
HpyF3I CTNAG 9 cut(s) 20, 350, 492, 689, 869, 1313, 1369, 1628, 1708
HpySE526I ACGT 3 cut(s) 82, 137, 524
Hsp92II CATG 3 cut(s) 278, 1111, 1233
HspAI GCGC 2 cut(s) 422, 551
KpnI GGTACC 1 cut(s) 439
Kzo9I GATC 5 cut(s) 77, 998, 1316, 1657, 1672
LmnI GCTCC 3 cut(s) 152, 849, 1417
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 5 cut(s) 38, 292, 846, 1070, 1242
MaeI CTAG 2 cut(s) 282, 683
MaeII ACGT 3 cut(s) 82, 137, 524
MaeIII GTNAC 2 cut(s) 1562, 1582
MalI GATC 5 cut(s) 79, 1000, 1318, 1659, 1674
MboI GATC 5 cut(s) 77, 998, 1316, 1657, 1672
MfeI CAATTG 5 cut(s) 417, 805, 914, 1556, 1645
MflI RGATCY 1 cut(s) 998
MlsI TGGCCA 1 cut(s) 924
MluNI TGGCCA 1 cut(s) 924
MlyI GAGTC 1 cut(s) 1468
MmeI TCCRAC 2 cut(s) 243, 1326
MnlI CCTC 8 cut(s) 278, 640, 1397, 1459, 1506, 1686, 1762, 1767
Mox20I TGGCCA 1 cut(s) 924
Mph1103I ATGCAT 2 cut(s) 1085, 1235
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 1 cut(s) 924
MslI CAYNNNNRTG 1 cut(s) 1749
Msp20I TGGCCA 1 cut(s) 924
MspA1I CMGCKG 2 cut(s) 1015, 1670
MspI CCGG 1 cut(s) 853
MspR9I CCNGG 1 cut(s) 1238
MunI CAATTG 5 cut(s) 417, 805, 914, 1556, 1645
MvaI CCWGG 1 cut(s) 1238
MvnI CGCG 1 cut(s) 424
MwoI GCNNNNNNNGC 3 cut(s) 254, 430, 1394
NdeII GATC 5 cut(s) 77, 998, 1316, 1657, 1672
NlaIII CATG 3 cut(s) 278, 1111, 1233
NlaIV GGNNCC 4 cut(s) 437, 851, 1000, 1622
NmeAIII GCCGAG 1 cut(s) 1657
NmuCI GTSAC 1 cut(s) 1582
NsiI ATGCAT 2 cut(s) 1085, 1235
NspI RCATGY 1 cut(s) 1233
NspV TTCGAA 2 cut(s) 220, 238
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 3 cut(s) 484, 1045, 1298
PfoI TCCNGGA 1 cut(s) 1236
PkrI GCNGC 1 cut(s) 256
PleI GAGTC 1 cut(s) 1467
PpsI GAGTC 1 cut(s) 1467
PshAI GACNNNNGTC 1 cut(s) 626
Psp6I CCWGG 1 cut(s) 1236
PspEI GGTNACC 2 cut(s) 1562, 1582
PspGI CCWGG 1 cut(s) 1236
PspN4I GGNNCC 4 cut(s) 437, 851, 1000, 1622
PspPI GGNCC 2 cut(s) 1621, 1754
PsrI GAACNNNNNNTAC 2 cut(s) 127, 159
PsuI RGATCY 1 cut(s) 998
PvuII CAGCTG 2 cut(s) 1015, 1670
RsaI GTAC 9 cut(s) 140, 145, 280, 437, 567, 898, 1228, 1477, 1643
RsaNI GTAC 9 cut(s) 139, 144, 279, 436, 566, 897, 1227, 1476, 1642
RseI CAYNNNNRTG 1 cut(s) 1749
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 5 cut(s) 77, 998, 1316, 1657, 1672
Sau96I GGNCC 2 cut(s) 1621, 1754
SchI GAGTC 1 cut(s) 1468
ScrFI CCNGG 1 cut(s) 1238
SfaNI GCATC 5 cut(s) 38, 292, 846, 1070, 1242
SfuI TTCGAA 2 cut(s) 220, 238
SinI GGWCC 2 cut(s) 1621, 1754
SmiMI CAYNNNNRTG 1 cut(s) 1749
SsiI CCGC 3 cut(s) 248, 424, 433
SspI AATATT 2 cut(s) 1026, 1378
SspMI CTAG 2 cut(s) 282, 683
StyD4I CCNGG 1 cut(s) 1236
StyI CCWWGG 1 cut(s) 1578
TaaI ACNGT 5 cut(s) 71, 143, 565, 906, 950
TaiI ACGT 3 cut(s) 85, 140, 527
TaqI TCGA 7 cut(s) 194, 220, 238, 339, 659, 1402, 1574
TatI WGTACW 4 cut(s) 565, 1226, 1475, 1641
TfiI GAWTC 3 cut(s) 484, 1045, 1298
TscAI CASTG 3 cut(s) 1213, 1395, 1756
TseFI GTSAC 1 cut(s) 1582
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 1582
TspDTI ATGAA 3 cut(s) 690, 1124, 1797
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 3 cut(s) 1213, 1395, 1756
VpaK11BI GGWCC 2 cut(s) 1621, 1754
XceI RCATGY 1 cut(s) 1233
XcmI CCANNNNNNNNNTGG 1 cut(s) 1576
XmiI GTMKAC 1 cut(s) 1362
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 2 cut(s) 282, 683
Zsp2I ATGCAT 2 cut(s) 1085, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.