mTERF
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 795 |
| AccII | CGCG | 1 cut(s) | 541 |
| AciI | CCGC | 3 cut(s) | 281, 311, 1337 |
| AclWI | GGATC | 2 cut(s) | 941, 1036 |
| AcoI | YGGCCR | 2 cut(s) | 98, 874 |
| AcsI | RAATTY | 4 cut(s) | 55, 944, 1010, 1734 |
| AcuI | CTGAAG | 4 cut(s) | 58, 426, 888, 1355 |
| AfaI | GTAC | 3 cut(s) | 380, 1132, 1592 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 96, 367, 1108 |
| AjnI | CCWGG | 2 cut(s) | 1188, 1624 |
| Alw21I | GWGCWC | 1 cut(s) | 1643 |
| Alw26I | GTCTC | 3 cut(s) | 52, 114, 644 |
| AlwI | GGATC | 2 cut(s) | 941, 1036 |
| AoxI | GGCC | 5 cut(s) | 98, 185, 268, 874, 1238 |
| ApeKI | GCWGC | 3 cut(s) | 236, 674, 846 |
| ApoI | RAATTY | 4 cut(s) | 55, 944, 1010, 1734 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 582 |
| AspLEI | GCGC | 2 cut(s) | 232, 541 |
| AsuC2I | CCSGG | 1 cut(s) | 376 |
| AsuHPI | GGTGA | 1 cut(s) | 300 |
| BaeGI | GKGCMC | 1 cut(s) | 124 |
| BalI | TGGCCA | 1 cut(s) | 876 |
| BauI | CACGAG | 1 cut(s) | 210 |
| Bbv12I | GWGCWC | 1 cut(s) | 1643 |
| BbvI | GCAGC | 3 cut(s) | 223, 661, 858 |
| BccI | CCATC | 4 cut(s) | 15, 36, 1153, 1356 |
| BciT130I | CCWGG | 2 cut(s) | 1190, 1626 |
| BcnI | CCSGG | 1 cut(s) | 376 |
| BcoDI | GTCTC | 3 cut(s) | 52, 114, 644 |
| BfaI | CTAG | 2 cut(s) | 132, 1179 |
| BfmI | CTRYAG | 2 cut(s) | 573, 983 |
| BglII | AGATCT | 1 cut(s) | 1660 |
| BisI | GCNGC | 3 cut(s) | 237, 675, 847 |
| BlsI | GCNGC | 3 cut(s) | 238, 676, 848 |
| Bme1390I | CCNGG | 3 cut(s) | 376, 1190, 1626 |
| BmiI | GGNNCC | 4 cut(s) | 88, 155, 740, 1114 |
| BmrFI | CCNGG | 3 cut(s) | 376, 1190, 1626 |
| BmrI | ACTGGG | 3 cut(s) | 111, 939, 1118 |
| BmsI | GCATC | 5 cut(s) | 38, 274, 798, 889, 941 |
| BmuI | ACTGGG | 3 cut(s) | 111, 939, 1118 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1245, 1277 |
| BpuEI | CTTGAG | 1 cut(s) | 1657 |
| BpuMI | CCSGG | 1 cut(s) | 376 |
| Bsa29I | ATCGAT | 1 cut(s) | 486 |
| BsaI | GGTCTC | 3 cut(s) | 52, 114, 644 |
| BsaJI | CCNNGG | 1 cut(s) | 742 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 96, 367, 1108 |
| Bse1I | ACTGG | 4 cut(s) | 117, 756, 934, 1113 |
| Bse3DI | GCAATG | 2 cut(s) | 1116, 1363 |
| BseBI | CCWGG | 2 cut(s) | 1190, 1626 |
| BseCI | ATCGAT | 1 cut(s) | 486 |
| BseDI | CCNNGG | 1 cut(s) | 742 |
| BseGI | GGATG | 9 cut(s) | 29, 289, 703, 813, 880, 956, 1185, 1367, 1693 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 96, 367, 1108 |
| BseMI | GCAATG | 2 cut(s) | 1116, 1363 |
| BseMII | CTCAG | 6 cut(s) | 52, 655, 812, 1276, 1664, 1671 |
| BseNI | ACTGG | 4 cut(s) | 117, 756, 934, 1113 |
| BseRI | GAGGAG | 2 cut(s) | 751, 1042 |
| BseSI | GKGCMC | 1 cut(s) | 124 |
| BseXI | GCAGC | 3 cut(s) | 223, 661, 858 |
| BseYI | CCCAGC | 3 cut(s) | 91, 634, 1249 |
| BsgI | GTGCAG | 1 cut(s) | 257 |
| Bsh1236I | CGCG | 1 cut(s) | 541 |
| BshFI | GGCC | 5 cut(s) | 100, 187, 270, 876, 1240 |
| BshVI | ATCGAT | 1 cut(s) | 486 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1643 |
| BsiSI | CCGG | 2 cut(s) | 361, 376 |
| BslFI | GGGAC | 1 cut(s) | 816 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 96, 367, 1108 |
| BsmAI | GTCTC | 3 cut(s) | 52, 114, 644 |
| BsmFI | GGGAC | 1 cut(s) | 816 |
| BsmI | GAATGC | 1 cut(s) | 1090 |
| BsnI | GGCC | 5 cut(s) | 100, 187, 270, 876, 1240 |
| Bso31I | GGTCTC | 3 cut(s) | 52, 114, 644 |
| Bsp1286I | GDGCHC | 2 cut(s) | 124, 1643 |
| Bsp1407I | TGTACA | 1 cut(s) | 1590 |
| Bsp143I | GATC | 8 cut(s) | 483, 933, 1041, 1265, 1408, 1489, 1606, 1660 |
| BspACI | CCGC | 3 cut(s) | 281, 311, 1337 |
| BspANI | GGCC | 5 cut(s) | 100, 187, 270, 876, 1240 |
| BspCNI | CTCAG | 6 cut(s) | 51, 654, 813, 1275, 1665, 1670 |
| BspDI | ATCGAT | 1 cut(s) | 486 |
| BspFNI | CGCG | 1 cut(s) | 541 |
| BspLI | GGNNCC | 4 cut(s) | 88, 155, 740, 1114 |
| BspMAI | CTGCAG | 1 cut(s) | 987 |
| BspPI | GGATC | 2 cut(s) | 941, 1036 |
| BspQI | GCTCTTC | 1 cut(s) | 624 |
| BspTNI | GGTCTC | 3 cut(s) | 52, 114, 644 |
| BsrDI | GCAATG | 2 cut(s) | 1116, 1363 |
| BsrGI | TGTACA | 1 cut(s) | 1590 |
| BsrI | ACTGG | 4 cut(s) | 117, 756, 934, 1113 |
| BssECI | CCNNGG | 1 cut(s) | 742 |
| BssMI | GATC | 8 cut(s) | 483, 933, 1041, 1265, 1408, 1489, 1606, 1660 |
| BssSI | CACGAG | 1 cut(s) | 210 |
| BssT1I | CCWWGG | 1 cut(s) | 742 |
| Bst2BI | CACGAG | 1 cut(s) | 210 |
| Bst2UI | CCWGG | 2 cut(s) | 1190, 1626 |
| Bst4CI | ACNGT | 1 cut(s) | 1618 |
| Bst6I | CTCTTC | 2 cut(s) | 55, 624 |
| BstAUI | TGTACA | 1 cut(s) | 1590 |
| BstC8I | GCNNGC | 3 cut(s) | 234, 272, 878 |
| BstDEI | CTNAG | 8 cut(s) | 38, 641, 821, 830, 1235, 1262, 1657, 1673 |
| BstF5I | GGATG | 9 cut(s) | 29, 289, 703, 813, 880, 956, 1185, 1367, 1693 |
| BstFNI | CGCG | 1 cut(s) | 541 |
| BstHHI | GCGC | 2 cut(s) | 232, 541 |
| BstKTI | GATC | 8 cut(s) | 486, 936, 1044, 1268, 1411, 1492, 1609, 1663 |
| BstMAI | GTCTC | 3 cut(s) | 52, 114, 644 |
| BstMBI | GATC | 8 cut(s) | 483, 933, 1041, 1265, 1408, 1489, 1606, 1660 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 236, 877, 960, 1246 |
| BstNI | CCWGG | 2 cut(s) | 1190, 1626 |
| BstSCI | CCNGG | 3 cut(s) | 374, 1188, 1624 |
| BstSFI | CTRYAG | 2 cut(s) | 573, 983 |
| BstSLI | GKGCMC | 1 cut(s) | 124 |
| BstUI | CGCG | 1 cut(s) | 541 |
| BstV1I | GCAGC | 3 cut(s) | 223, 661, 858 |
| BstX2I | RGATCY | 2 cut(s) | 933, 1660 |
| BstYI | RGATCY | 2 cut(s) | 933, 1660 |
| Bsu15I | ATCGAT | 1 cut(s) | 486 |
| BsuRI | GGCC | 5 cut(s) | 100, 187, 270, 876, 1240 |
| BsuTUI | ATCGAT | 1 cut(s) | 486 |
| BtsCI | GGATG | 9 cut(s) | 29, 289, 703, 813, 880, 956, 1185, 1367, 1693 |
| BtsIMutI | CAGTG | 1 cut(s) | 124 |
| Cac8I | GCNNGC | 3 cut(s) | 234, 272, 878 |
| CfoI | GCGC | 2 cut(s) | 232, 541 |
| ClaI | ATCGAT | 1 cut(s) | 486 |
| CsiI | ACCWGGT | 1 cut(s) | 1624 |
| Csp6I | GTAC | 3 cut(s) | 379, 1131, 1591 |
| CviAII | CATG | 4 cut(s) | 475, 565, 1049, 1588 |
| CviQI | GTAC | 3 cut(s) | 379, 1131, 1591 |
| DdeI | CTNAG | 8 cut(s) | 38, 641, 821, 830, 1235, 1262, 1657, 1673 |
| DpnI | GATC | 8 cut(s) | 485, 935, 1043, 1267, 1410, 1491, 1608, 1662 |
| DpnII | GATC | 8 cut(s) | 483, 933, 1041, 1265, 1408, 1489, 1606, 1660 |
| EaeI | YGGCCR | 2 cut(s) | 98, 874 |
| Eam1104I | CTCTTC | 2 cut(s) | 55, 624 |
| EarI | CTCTTC | 2 cut(s) | 55, 624 |
| Eco130I | CCWWGG | 1 cut(s) | 742 |
| Eco147I | AGGCCT | 1 cut(s) | 270 |
| Eco31I | GGTCTC | 3 cut(s) | 52, 114, 644 |
| Eco32I | GATATC | 2 cut(s) | 1442, 1547 |
| Eco57I | CTGAAG | 4 cut(s) | 58, 426, 888, 1355 |
| EcoRII | CCWGG | 2 cut(s) | 1188, 1624 |
| EcoRV | GATATC | 2 cut(s) | 1442, 1547 |
| EcoT14I | CCWWGG | 1 cut(s) | 742 |
| ErhI | CCWWGG | 1 cut(s) | 742 |
| FaeI | CATG | 4 cut(s) | 478, 568, 1052, 1591 |
| FaqI | GGGAC | 1 cut(s) | 816 |
| FatI | CATG | 4 cut(s) | 474, 564, 1048, 1587 |
| Fnu4HI | GCNGC | 3 cut(s) | 237, 675, 847 |
| FokI | GGATG | 9 cut(s) | 16, 296, 710, 820, 867, 963, 1172, 1374, 1680 |
| Fsp4HI | GCNGC | 3 cut(s) | 237, 675, 847 |
| FspBI | CTAG | 2 cut(s) | 132, 1179 |
| GlaI | GCGC | 2 cut(s) | 231, 540 |
| GluI | GCNGC | 3 cut(s) | 237, 675, 847 |
| GsaI | CCCAGC | 3 cut(s) | 95, 638, 1253 |
| HaeIII | GGCC | 5 cut(s) | 100, 187, 270, 876, 1240 |
| HapII | CCGG | 2 cut(s) | 361, 376 |
| HhaI | GCGC | 2 cut(s) | 232, 541 |
| Hin1II | CATG | 4 cut(s) | 478, 568, 1052, 1591 |
| Hin6I | GCGC | 2 cut(s) | 230, 539 |
| HinP1I | GCGC | 2 cut(s) | 230, 539 |
| HincII | GTYRAC | 1 cut(s) | 1524 |
| HindII | GTYRAC | 1 cut(s) | 1524 |
| HindIII | AAGCTT | 1 cut(s) | 326 |
| HinfI | GANTC | 3 cut(s) | 493, 782, 788 |
| HpaII | CCGG | 2 cut(s) | 361, 376 |
| HphI | GGTGA | 1 cut(s) | 300 |
| Hpy166II | GTNNAC | 3 cut(s) | 1068, 1427, 1524 |
| Hpy188III | TCNNGA | 6 cut(s) | 481, 884, 1179, 1373, 1412, 1706 |
| Hpy8I | GTNNAC | 3 cut(s) | 1068, 1427, 1524 |
| HpyAV | CCTTC | 4 cut(s) | 450, 906, 1162, 1546 |
| HpyCH4III | ACNGT | 1 cut(s) | 1618 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 236, 877, 960, 1246 |
| HpyF3I | CTNAG | 8 cut(s) | 38, 641, 821, 830, 1235, 1262, 1657, 1673 |
| Hsp92II | CATG | 4 cut(s) | 478, 568, 1052, 1591 |
| HspAI | GCGC | 2 cut(s) | 230, 539 |
| Kzo9I | GATC | 8 cut(s) | 483, 933, 1041, 1265, 1408, 1489, 1606, 1660 |
| LguI | GCTCTTC | 1 cut(s) | 624 |
| LmnI | GCTCC | 4 cut(s) | 682, 738, 1171, 1366 |
| Lsp1109I | GCAGC | 3 cut(s) | 223, 661, 858 |
| LweI | GCATC | 5 cut(s) | 38, 274, 798, 889, 941 |
| MabI | ACCWGGT | 1 cut(s) | 1624 |
| MaeI | CTAG | 2 cut(s) | 132, 1179 |
| MaeIII | GTNAC | 2 cut(s) | 160, 1003 |
| MalI | GATC | 8 cut(s) | 485, 935, 1043, 1267, 1410, 1491, 1608, 1662 |
| MboI | GATC | 8 cut(s) | 483, 933, 1041, 1265, 1408, 1489, 1606, 1660 |
| MboII | GAAGA | 9 cut(s) | 42, 452, 568, 641, 1093, 1139, 1222, 1495, 1738 |
| MfeI | CAATTG | 2 cut(s) | 102, 1594 |
| MflI | RGATCY | 2 cut(s) | 933, 1660 |
| MhlI | GDGCHC | 2 cut(s) | 124, 1643 |
| MlsI | TGGCCA | 1 cut(s) | 876 |
| MluNI | TGGCCA | 1 cut(s) | 876 |
| MlyI | GAGTC | 1 cut(s) | 502 |
| MmeI | TCCRAC | 2 cut(s) | 225, 705 |
| Mox20I | TGGCCA | 1 cut(s) | 876 |
| MroXI | GAANNNNTTC | 1 cut(s) | 582 |
| MscI | TGGCCA | 1 cut(s) | 876 |
| MseI | TTAA | 6 cut(s) | 399, 597, 660, 1154, 1434, 1563 |
| MslI | CAYNNNNRTG | 2 cut(s) | 510, 1698 |
| Msp20I | TGGCCA | 1 cut(s) | 876 |
| MspI | CCGG | 2 cut(s) | 361, 376 |
| MspR9I | CCNGG | 3 cut(s) | 376, 1190, 1626 |
| MunI | CAATTG | 2 cut(s) | 102, 1594 |
| Mva1269I | GAATGC | 1 cut(s) | 1090 |
| MvaI | CCWGG | 2 cut(s) | 1190, 1626 |
| MvnI | CGCG | 1 cut(s) | 541 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 236, 877, 960, 1246 |
| NciI | CCSGG | 1 cut(s) | 376 |
| NdeII | GATC | 8 cut(s) | 483, 933, 1041, 1265, 1408, 1489, 1606, 1660 |
| NlaIII | CATG | 4 cut(s) | 478, 568, 1052, 1591 |
| NlaIV | GGNNCC | 4 cut(s) | 88, 155, 740, 1114 |
| NmeAIII | GCCGAG | 1 cut(s) | 76 |
| NmuCI | GTSAC | 1 cut(s) | 160 |
| PceI | AGGCCT | 1 cut(s) | 270 |
| PciSI | GCTCTTC | 1 cut(s) | 624 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 208, 217 |
| PctI | GAATGC | 1 cut(s) | 1090 |
| PdmI | GAANNNNTTC | 1 cut(s) | 582 |
| PfeI | GAWTC | 2 cut(s) | 782, 788 |
| PfoI | TCCNGGA | 1 cut(s) | 1188 |
| PkrI | GCNGC | 3 cut(s) | 238, 676, 848 |
| PleI | GAGTC | 1 cut(s) | 501 |
| PpsI | GAGTC | 1 cut(s) | 501 |
| PsiI | TTATAA | 1 cut(s) | 795 |
| Psp6I | CCWGG | 2 cut(s) | 1188, 1624 |
| PspFI | CCCAGC | 3 cut(s) | 91, 634, 1249 |
| PspGI | CCWGG | 2 cut(s) | 1188, 1624 |
| PspN4I | GGNNCC | 4 cut(s) | 88, 155, 740, 1114 |
| PstI | CTGCAG | 1 cut(s) | 987 |
| PsuI | RGATCY | 2 cut(s) | 933, 1660 |
| RsaI | GTAC | 3 cut(s) | 380, 1132, 1592 |
| RsaNI | GTAC | 3 cut(s) | 379, 1131, 1591 |
| RseI | CAYNNNNRTG | 2 cut(s) | 510, 1698 |
| SapI | GCTCTTC | 1 cut(s) | 624 |
| SaqAI | TTAA | 6 cut(s) | 399, 597, 660, 1154, 1434, 1563 |
| SatI | GCNGC | 3 cut(s) | 237, 675, 847 |
| Sau3AI | GATC | 8 cut(s) | 483, 933, 1041, 1265, 1408, 1489, 1606, 1660 |
| SchI | GAGTC | 1 cut(s) | 502 |
| ScrFI | CCNGG | 3 cut(s) | 376, 1190, 1626 |
| SduI | GDGCHC | 2 cut(s) | 124, 1643 |
| SexAI | ACCWGGT | 1 cut(s) | 1624 |
| SfaNI | GCATC | 5 cut(s) | 38, 274, 798, 889, 941 |
| SfcI | CTRYAG | 2 cut(s) | 573, 983 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 510, 1698 |
| SmlI | CTYRAG | 1 cut(s) | 1636 |
| SmoI | CTYRAG | 1 cut(s) | 1636 |
| SseBI | AGGCCT | 1 cut(s) | 270 |
| SsiI | CCGC | 3 cut(s) | 281, 311, 1337 |
| SspMI | CTAG | 2 cut(s) | 132, 1179 |
| StuI | AGGCCT | 1 cut(s) | 270 |
| StyD4I | CCNGG | 3 cut(s) | 374, 1188, 1624 |
| StyI | CCWWGG | 1 cut(s) | 742 |
| TaaI | ACNGT | 1 cut(s) | 1618 |
| TaqI | TCGA | 6 cut(s) | 202, 220, 486, 586, 619, 646 |
| TatI | WGTACW | 1 cut(s) | 1590 |
| TfiI | GAWTC | 2 cut(s) | 782, 788 |
| Tru1I | TTAA | 6 cut(s) | 399, 597, 660, 1154, 1434, 1563 |
| Tru9I | TTAA | 6 cut(s) | 399, 597, 660, 1154, 1434, 1563 |
| TscAI | CASTG | 1 cut(s) | 124 |
| TseFI | GTSAC | 1 cut(s) | 160 |
| TseI | GCWGC | 3 cut(s) | 236, 674, 846 |
| Tsp45I | GTSAC | 1 cut(s) | 160 |
| TspDTI | ATGAA | 9 cut(s) | 78, 443, 642, 1065, 1223, 1298, 1431, 1496, 1576 |
| TspGWI | ACGGA | 1 cut(s) | 820 |
| TspRI | CASTG | 1 cut(s) | 124 |
| XapI | RAATTY | 4 cut(s) | 55, 944, 1010, 1734 |
| XbaI | TCTAGA | 1 cut(s) | 1178 |
| XmnI | GAANNNNTTC | 1 cut(s) | 582 |
| XspI | CTAG | 2 cut(s) | 132, 1179 |