mTERF
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1694 |
| Acc65I | GGTACC | 1 cut(s) | 405 |
| AccB1I | GGYRCC | 1 cut(s) | 405 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1582 |
| AciI | CCGC | 4 cut(s) | 382, 403, 557, 1010 |
| AclWI | GGATC | 1 cut(s) | 1437 |
| AcsI | RAATTY | 4 cut(s) | 422, 962, 1028, 1039 |
| AcuI | CTGAAG | 2 cut(s) | 1376, 1382 |
| AfaI | GTAC | 5 cut(s) | 280, 407, 1168, 1605, 1707 |
| AfeI | AGCGCT | 1 cut(s) | 1359 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1126, 1271, 1486, 1582 |
| AjnI | CCWGG | 3 cut(s) | 1062, 1206, 1645 |
| Alw21I | GWGCWC | 2 cut(s) | 1139, 1664 |
| Alw26I | GTCTC | 2 cut(s) | 1039, 1200 |
| AlwI | GGATC | 1 cut(s) | 1437 |
| Aor51HI | AGCGCT | 1 cut(s) | 1359 |
| AoxI | GGCC | 1 cut(s) | 153 |
| ApeKI | GCWGC | 5 cut(s) | 254, 1085, 1155, 1355, 1640 |
| ApoI | RAATTY | 4 cut(s) | 422, 962, 1028, 1039 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1310, 1342 |
| AseI | ATTAAT | 1 cut(s) | 417 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1452 |
| Asp718I | GGTACC | 1 cut(s) | 405 |
| AspLEI | GCGC | 1 cut(s) | 1360 |
| AspS9I | GGNCC | 2 cut(s) | 154, 1724 |
| AsuHPI | GGTGA | 3 cut(s) | 62, 72, 1106 |
| AvaII | GGWCC | 1 cut(s) | 1724 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1595, 1628 |
| BanI | GGYRCC | 1 cut(s) | 405 |
| Bbv12I | GWGCWC | 2 cut(s) | 1139, 1664 |
| BbvI | GCAGC | 5 cut(s) | 241, 1072, 1167, 1367, 1627 |
| BccI | CCATC | 1 cut(s) | 850 |
| BceAI | ACGGC | 1 cut(s) | 648 |
| BciT130I | CCWGG | 3 cut(s) | 1064, 1208, 1647 |
| BcoDI | GTCTC | 2 cut(s) | 1039, 1200 |
| BfaI | CTAG | 4 cut(s) | 282, 338, 653, 1152 |
| BfmI | CTRYAG | 1 cut(s) | 1086 |
| BfoI | RGCGCY | 1 cut(s) | 1361 |
| BisI | GCNGC | 5 cut(s) | 255, 1086, 1156, 1356, 1641 |
| BlsI | GCNGC | 5 cut(s) | 256, 1087, 1157, 1357, 1642 |
| BmcAI | AGTACT | 1 cut(s) | 280 |
| Bme1390I | CCNGG | 3 cut(s) | 1064, 1208, 1647 |
| Bme18I | GGWCC | 1 cut(s) | 1724 |
| BmgT120I | GGNCC | 2 cut(s) | 154, 1724 |
| BmiI | GGNNCC | 3 cut(s) | 156, 407, 821 |
| BmrFI | CCNGG | 3 cut(s) | 1064, 1208, 1647 |
| BmrI | ACTGGG | 1 cut(s) | 1136 |
| BmsI | GCATC | 4 cut(s) | 38, 292, 635, 816 |
| BmuI | ACTGGG | 1 cut(s) | 1136 |
| BpmI | CTGGAG | 1 cut(s) | 1324 |
| Bpu10I | CCTNAGC | 2 cut(s) | 201, 1598 |
| BpuEI | CTTGAG | 2 cut(s) | 1678, 1713 |
| BsaJI | CCNNGG | 1 cut(s) | 1646 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1266, 1296 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1126, 1271, 1486, 1582 |
| Bse1I | ACTGG | 2 cut(s) | 1019, 1131 |
| Bse3DI | GCAATG | 1 cut(s) | 1384 |
| BseBI | CCWGG | 3 cut(s) | 1064, 1208, 1647 |
| BseDI | CCNNGG | 1 cut(s) | 1646 |
| BseGI | GGATG | 7 cut(s) | 721, 831, 1072, 1203, 1444, 1495, 1714 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1126, 1271, 1486, 1582 |
| BseMI | GCAATG | 1 cut(s) | 1384 |
| BseMII | CTCAG | 3 cut(s) | 34, 297, 1692 |
| BseNI | ACTGG | 2 cut(s) | 1019, 1131 |
| BseRI | GAGGAG | 1 cut(s) | 1400 |
| BseXI | GCAGC | 5 cut(s) | 241, 1072, 1167, 1367, 1627 |
| BseYI | CCCAGC | 2 cut(s) | 38, 42 |
| Bsh1285I | CGRYCG | 1 cut(s) | 386 |
| BshFI | GGCC | 1 cut(s) | 155 |
| BshNI | GGYRCC | 1 cut(s) | 405 |
| BsiEI | CGRYCG | 1 cut(s) | 386 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1139, 1664 |
| BsiSI | CCGG | 1 cut(s) | 823 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1126, 1271, 1486, 1582 |
| BsmAI | GTCTC | 2 cut(s) | 1039, 1200 |
| BsmBI | CGTCTC | 1 cut(s) | 1039 |
| BsmI | GAATGC | 1 cut(s) | 1053 |
| BsnI | GGCC | 1 cut(s) | 155 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1139, 1664 |
| Bsp1407I | TGTACA | 1 cut(s) | 1705 |
| Bsp143I | GATC | 5 cut(s) | 77, 1286, 1429, 1506, 1627 |
| BspACI | CCGC | 4 cut(s) | 382, 403, 557, 1010 |
| BspANI | GGCC | 1 cut(s) | 155 |
| BspCNI | CTCAG | 3 cut(s) | 33, 298, 1691 |
| BspHI | TCATGA | 1 cut(s) | 61 |
| BspLI | GGNNCC | 3 cut(s) | 156, 407, 821 |
| BspMAI | CTGCAG | 1 cut(s) | 1090 |
| BspPI | GGATC | 1 cut(s) | 1437 |
| BspT107I | GGYRCC | 1 cut(s) | 405 |
| BsrDI | GCAATG | 1 cut(s) | 1384 |
| BsrGI | TGTACA | 1 cut(s) | 1705 |
| BsrI | ACTGG | 2 cut(s) | 1019, 1131 |
| BssECI | CCNNGG | 1 cut(s) | 1646 |
| BssMI | GATC | 5 cut(s) | 77, 1286, 1429, 1506, 1627 |
| Bst2UI | CCWGG | 3 cut(s) | 1064, 1208, 1647 |
| Bst4CI | ACNGT | 5 cut(s) | 278, 535, 937, 1050, 1514 |
| Bst6I | CTCTTC | 4 cut(s) | 231, 480, 749, 971 |
| BstAUI | TGTACA | 1 cut(s) | 1705 |
| BstC8I | GCNNGC | 2 cut(s) | 252, 884 |
| BstDEI | CTNAG | 8 cut(s) | 20, 74, 201, 306, 659, 1305, 1598, 1678 |
| BstF5I | GGATG | 7 cut(s) | 721, 831, 1072, 1203, 1444, 1495, 1714 |
| BstH2I | RGCGCY | 1 cut(s) | 1361 |
| BstHHI | GCGC | 1 cut(s) | 1360 |
| BstKTI | GATC | 5 cut(s) | 80, 1289, 1432, 1509, 1630 |
| BstMAI | GTCTC | 2 cut(s) | 1039, 1200 |
| BstMBI | GATC | 5 cut(s) | 77, 1286, 1429, 1506, 1627 |
| BstMCI | CGRYCG | 1 cut(s) | 386 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1364 |
| BstNI | CCWGG | 3 cut(s) | 1064, 1208, 1647 |
| BstNSI | RCATGY | 2 cut(s) | 1371, 1612 |
| BstSCI | CCNGG | 3 cut(s) | 1062, 1206, 1645 |
| BstSFI | CTRYAG | 1 cut(s) | 1086 |
| BstV1I | GCAGC | 5 cut(s) | 241, 1072, 1167, 1367, 1627 |
| BsuRI | GGCC | 1 cut(s) | 155 |
| BtsCI | GGATG | 7 cut(s) | 721, 831, 1072, 1203, 1444, 1495, 1714 |
| BtsI | GCAGTG | 1 cut(s) | 871 |
| BtsIMutI | CAGTG | 3 cut(s) | 297, 871, 941 |
| Cac8I | GCNNGC | 2 cut(s) | 252, 884 |
| CciI | TCATGA | 1 cut(s) | 61 |
| CfoI | GCGC | 1 cut(s) | 1360 |
| Cfr13I | GGNCC | 2 cut(s) | 154, 1724 |
| Csp6I | GTAC | 5 cut(s) | 279, 406, 1167, 1604, 1706 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 471, 506 |
| CviAII | CATG | 7 cut(s) | 62, 173, 1078, 1368, 1461, 1609, 1631 |
| CviQI | GTAC | 5 cut(s) | 279, 406, 1167, 1604, 1706 |
| DdeI | CTNAG | 8 cut(s) | 20, 74, 201, 306, 659, 1305, 1598, 1678 |
| DpnI | GATC | 5 cut(s) | 79, 1288, 1431, 1508, 1629 |
| DpnII | GATC | 5 cut(s) | 77, 1286, 1429, 1506, 1627 |
| Eam1104I | CTCTTC | 4 cut(s) | 231, 480, 749, 971 |
| EarI | CTCTTC | 4 cut(s) | 231, 480, 749, 971 |
| EciI | GGCGGA | 1 cut(s) | 546 |
| Eco32I | GATATC | 1 cut(s) | 1568 |
| Eco47I | GGWCC | 1 cut(s) | 1724 |
| Eco47III | AGCGCT | 1 cut(s) | 1359 |
| Eco57I | CTGAAG | 2 cut(s) | 1376, 1382 |
| EcoRII | CCWGG | 3 cut(s) | 1062, 1206, 1645 |
| EcoRV | GATATC | 1 cut(s) | 1568 |
| Esp3I | CGTCTC | 1 cut(s) | 1039 |
| FaeI | CATG | 7 cut(s) | 65, 176, 1081, 1371, 1464, 1612, 1634 |
| FatI | CATG | 7 cut(s) | 61, 172, 1077, 1367, 1460, 1608, 1630 |
| FauNDI | CATATG | 1 cut(s) | 9 |
| Fnu4HI | GCNGC | 5 cut(s) | 255, 1086, 1156, 1356, 1641 |
| FokI | GGATG | 7 cut(s) | 728, 838, 1079, 1190, 1451, 1502, 1701 |
| Fsp4HI | GCNGC | 5 cut(s) | 255, 1086, 1156, 1356, 1641 |
| FspBI | CTAG | 4 cut(s) | 282, 338, 653, 1152 |
| GlaI | GCGC | 1 cut(s) | 1359 |
| GluI | GCNGC | 5 cut(s) | 255, 1086, 1156, 1356, 1641 |
| GsaI | CCCAGC | 2 cut(s) | 42, 46 |
| GsuI | CTGGAG | 1 cut(s) | 1324 |
| HaeII | RGCGCY | 1 cut(s) | 1361 |
| HaeIII | GGCC | 1 cut(s) | 155 |
| HapII | CCGG | 1 cut(s) | 823 |
| HhaI | GCGC | 1 cut(s) | 1360 |
| Hin1II | CATG | 7 cut(s) | 65, 176, 1081, 1371, 1464, 1612, 1634 |
| Hin6I | GCGC | 1 cut(s) | 1358 |
| HinP1I | GCGC | 1 cut(s) | 1358 |
| HincII | GTYRAC | 1 cut(s) | 1554 |
| HindII | GTYRAC | 1 cut(s) | 1554 |
| HindIII | AAGCTT | 3 cut(s) | 344, 639, 1595 |
| HinfI | GANTC | 7 cut(s) | 553, 790, 1015, 1256, 1268, 1453, 1760 |
| HpaII | CCGG | 1 cut(s) | 823 |
| HphI | GGTGA | 3 cut(s) | 62, 72, 1106 |
| Hpy166II | GTNNAC | 3 cut(s) | 139, 1448, 1554 |
| Hpy188I | TCNGA | 3 cut(s) | 463, 1434, 1681 |
| Hpy188III | TCNNGA | 6 cut(s) | 22, 62, 338, 1265, 1272, 1394 |
| Hpy8I | GTNNAC | 3 cut(s) | 139, 1448, 1554 |
| HpyAV | CCTTC | 5 cut(s) | 442, 468, 1075, 1357, 1429 |
| HpyCH4III | ACNGT | 5 cut(s) | 278, 535, 937, 1050, 1514 |
| HpyCH4V | TGCA | 9 cut(s) | 13, 257, 274, 725, 864, 1037, 1053, 1088, 1410 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1364 |
| HpyF3I | CTNAG | 8 cut(s) | 20, 74, 201, 306, 659, 1305, 1598, 1678 |
| Hsp92II | CATG | 7 cut(s) | 65, 176, 1081, 1371, 1464, 1612, 1634 |
| HspAI | GCGC | 1 cut(s) | 1358 |
| KpnI | GGTACC | 1 cut(s) | 409 |
| Kzo9I | GATC | 5 cut(s) | 77, 1286, 1429, 1506, 1627 |
| LmnI | GCTCC | 3 cut(s) | 819, 1387, 1648 |
| Lsp1109I | GCAGC | 5 cut(s) | 241, 1072, 1167, 1367, 1627 |
| LweI | GCATC | 4 cut(s) | 38, 292, 635, 816 |
| MaeI | CTAG | 4 cut(s) | 282, 338, 653, 1152 |
| MaeIII | GTNAC | 3 cut(s) | 230, 893, 954 |
| MalI | GATC | 5 cut(s) | 79, 1288, 1431, 1508, 1629 |
| MboI | GATC | 5 cut(s) | 77, 1286, 1429, 1506, 1627 |
| MfeI | CAATTG | 3 cut(s) | 102, 1615, 1765 |
| MhlI | GDGCHC | 2 cut(s) | 1139, 1664 |
| MlyI | GAGTC | 1 cut(s) | 1265 |
| MmeI | TCCRAC | 1 cut(s) | 243 |
| MnlI | CCTC | 7 cut(s) | 278, 589, 778, 1367, 1378, 1476, 1737 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1452 |
| MseI | TTAA | 2 cut(s) | 417, 1476 |
| MslI | CAYNNNNRTG | 1 cut(s) | 149 |
| MspA1I | CMGCKG | 1 cut(s) | 42 |
| MspI | CCGG | 1 cut(s) | 823 |
| MspR9I | CCNGG | 3 cut(s) | 1064, 1208, 1647 |
| MunI | CAATTG | 3 cut(s) | 102, 1615, 1765 |
| Mva1269I | GAATGC | 1 cut(s) | 1053 |
| MvaI | CCWGG | 3 cut(s) | 1064, 1208, 1647 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1364 |
| NdeI | CATATG | 1 cut(s) | 9 |
| NdeII | GATC | 5 cut(s) | 77, 1286, 1429, 1506, 1627 |
| NlaIII | CATG | 7 cut(s) | 65, 176, 1081, 1371, 1464, 1612, 1634 |
| NlaIV | GGNNCC | 3 cut(s) | 156, 407, 821 |
| NmuCI | GTSAC | 1 cut(s) | 893 |
| NspI | RCATGY | 2 cut(s) | 1371, 1612 |
| PagI | TCATGA | 1 cut(s) | 61 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 226 |
| PctI | GAATGC | 1 cut(s) | 1053 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1452 |
| PfeI | GAWTC | 6 cut(s) | 553, 790, 1015, 1268, 1453, 1760 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1582 |
| PfoI | TCCNGGA | 2 cut(s) | 1062, 1206 |
| PkrI | GCNGC | 5 cut(s) | 256, 1087, 1157, 1357, 1642 |
| PleI | GAGTC | 1 cut(s) | 1264 |
| PpsI | GAGTC | 1 cut(s) | 1264 |
| PshBI | ATTAAT | 1 cut(s) | 417 |
| PsiI | TTATAA | 1 cut(s) | 1694 |
| Psp6I | CCWGG | 3 cut(s) | 1062, 1206, 1645 |
| PspFI | CCCAGC | 2 cut(s) | 38, 42 |
| PspGI | CCWGG | 3 cut(s) | 1062, 1206, 1645 |
| PspN4I | GGNNCC | 3 cut(s) | 156, 407, 821 |
| PspPI | GGNCC | 2 cut(s) | 154, 1724 |
| PstI | CTGCAG | 1 cut(s) | 1090 |
| PvuII | CAGCTG | 1 cut(s) | 42 |
| RsaI | GTAC | 5 cut(s) | 280, 407, 1168, 1605, 1707 |
| RsaNI | GTAC | 5 cut(s) | 279, 406, 1167, 1604, 1706 |
| RseI | CAYNNNNRTG | 1 cut(s) | 149 |
| SaqAI | TTAA | 2 cut(s) | 417, 1476 |
| SatI | GCNGC | 5 cut(s) | 255, 1086, 1156, 1356, 1641 |
| Sau3AI | GATC | 5 cut(s) | 77, 1286, 1429, 1506, 1627 |
| Sau96I | GGNCC | 2 cut(s) | 154, 1724 |
| ScaI | AGTACT | 1 cut(s) | 280 |
| SchI | GAGTC | 1 cut(s) | 1265 |
| ScrFI | CCNGG | 3 cut(s) | 1064, 1208, 1647 |
| SduI | GDGCHC | 2 cut(s) | 1139, 1664 |
| SfaNI | GCATC | 4 cut(s) | 38, 292, 635, 816 |
| SfcI | CTRYAG | 1 cut(s) | 1086 |
| SinI | GGWCC | 1 cut(s) | 1724 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 149 |
| SmlI | CTYRAG | 2 cut(s) | 1657, 1728 |
| SmoI | CTYRAG | 2 cut(s) | 1657, 1728 |
| SsiI | CCGC | 4 cut(s) | 382, 403, 557, 1010 |
| SspI | AATATT | 1 cut(s) | 313 |
| SspMI | CTAG | 4 cut(s) | 282, 338, 653, 1152 |
| StyD4I | CCNGG | 3 cut(s) | 1062, 1206, 1645 |
| TaaI | ACNGT | 5 cut(s) | 278, 535, 937, 1050, 1514 |
| TaqI | TCGA | 5 cut(s) | 194, 220, 386, 1372, 1509 |
| TatI | WGTACW | 3 cut(s) | 278, 1166, 1705 |
| TfiI | GAWTC | 6 cut(s) | 553, 790, 1015, 1268, 1453, 1760 |
| Tru1I | TTAA | 2 cut(s) | 417, 1476 |
| Tru9I | TTAA | 2 cut(s) | 417, 1476 |
| TscAI | CASTG | 3 cut(s) | 304, 871, 948 |
| TseFI | GTSAC | 1 cut(s) | 893 |
| TseI | GCWGC | 5 cut(s) | 254, 1085, 1155, 1355, 1640 |
| Tsp45I | GTSAC | 1 cut(s) | 893 |
| TspGWI | ACGGA | 1 cut(s) | 1502 |
| TspRI | CASTG | 3 cut(s) | 304, 871, 948 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1582 |
| VpaK11BI | GGWCC | 1 cut(s) | 1724 |
| VspI | ATTAAT | 1 cut(s) | 417 |
| XapI | RAATTY | 4 cut(s) | 422, 962, 1028, 1039 |
| XbaI | TCTAGA | 1 cut(s) | 337 |
| XceI | RCATGY | 2 cut(s) | 1371, 1612 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1452 |
| XspI | CTAG | 4 cut(s) | 282, 338, 653, 1152 |
| ZrmI | AGTACT | 1 cut(s) | 280 |