pycom12g05300
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001228
Physical Location & Seq
Forward (+)
5145037 .. 5146839
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05300.1

Sequence Viewer

Length: 1803 bp
ATGACCCATTTGCAGAAACTCAGATTAGCATCTGTTCGCAAATGGGTTTCGTTGAATTGTTGCGAAAACCGTGTTAGATCACCTTTAATCCCATTTCAGCCAATTGGGTATTTCTGCAATGCCCCAACCATTAGAACGTACAGTACCAATGGAGCTTCTGCAAATGAGAATTCCGAAAGTTCGGATAAAACTTCGAGTGATAAGGGAAGAAATGCTGTTCGAATTTCTTATTCCATTCGAAAAGAAGCGGAAGCTGCATTGTTGGATTATTTGCATGGTACTAGAGGGTTGCAGTTCACGGATGCAGAGAATATGAGTAAAAACTCGCCTCGCTTTCTCGACAAGCTTCTGAGACAGGTTGATATTGAAAAAGAAATTCTTGCAAAGAAGAATAATCAGAAAGATATTGGGCGGGCAATTGCGCGGTTCTTGCGGTACCATCCTATTAACGAATTTGAGCCTTTCTTTGAGAGCTTGGGTTTGAATCCTTCTGAGTATTCTCCGCTTCTTCCACGCAGTTTGACGTTTTTGACTGATGATAAGTTATTAGTGCACAATTATACTGTACTATGTGATTATGGAATTGCACGCAGTAAGATAGGAAAGATTTACAAGGAATGGACAGAAGTCTTTCAATATGACTTTGAGGTTTTAACATCGAAACTTAAAGCTTATGAAGAACTAGGTCTTAGCCAATCTACTTTTGGTAAGTTTATAGTTGCTACTCCTTGTCTTTTAATTGGGGATGTAAATGTTGAATTTGTTAAGGTTTTGGAAAAATTGCAGAGTTTTGGATTTAAAACCATTTGGATTGAAGGTAATTTGCCAGCAGATAGTTCTCATAATTGGAGCCGGATGCTTGAAGTTCTTAGTTTGTTTAGTGAGATGGGTTGCAGTACCGAACAGTTGGGTCAATTGATTGGTCAGCACCCAGATATTTTGTTTGATGGTTCTGGTGAAAGGACGCTTTCACTAATTGGGTTCTTACTGAAATTTGGATCCACAAAAAGCCAGCTGTGTTCAATATTTCTGCAATTTCCACAGATTCCAGTTGTGAAATTTGTTTCAAATTTAAGACAATGCATTTTGTTCCTGAATGAGATTGACATGAATGTTGCAGAGATTGGGAAGATTGTCCATTCCCACCCCCTGCTTCTCGGTTCTTGTTCTTTGAAAAAAACCGTCAGTTTACTCTCAAACTTGAACGCTGGGAAGAAAAGATTGTGTACATGCATCCAGAAGAACCCACAAGTATTGAAGAATTGGGTTTTGGGTAAAAGAGTTGAGCGAATTCCAGACTCGGGAGACGACCTTAGATCAAAGATACAAAAGATTAAGTTTTTGTTGGATGCAGGATTTGTAGACAACTCAGACAATATTAAAGAAGCACTGAAAGCATTTCGCGGCAAGGGAATGGAGCTTCAGGAAAGATTTGATTGTATTGTTAAAGCTGGTTTAAGTCCAGAGGATGTCTGTACAATGATTAAACTAAGCCCTCAAATTCTTAACCAGACAAAGGATATCATTGAAGAGAAGATTGATTTTCTTGTAAATCAATTGGGTTACCCCATTTCGACTTTGGTGACCTTCCCAAGATATCTTTCCTATAAGATTGAAAGGGTCCAGCTTAGGACATTGATGTACAATTGGCTCAAGGATCATGGAACAGCTGATCCTCGGCTTAACCTTAGCACTATTATTTGTTGCTCAGATAAATGTTTTGTTAGTTCTTATGTAAATCATCATCCTAGTGGTCCTCAAGTGTGGGAGGATTTGAAGAATGAAATTTATTCCAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

601

Amino Acids

68.16

Weight (kDa)

8.74

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 290 - 428 8.1e-14 mTERF
mTERF PF02536 473 - 574 1.8e-15 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 435
AccI GTMKAC 1 cut(s) 1362
AccII CGCG 2 cut(s) 424, 1404
AciI CCGC 6 cut(s) 248, 412, 424, 433, 503, 1404
AclWI GGATC 4 cut(s) 993, 1006, 1665, 1667
AcuI CTGAAG 1 cut(s) 1406
AfaI GTAC 9 cut(s) 140, 145, 280, 437, 567, 898, 1228, 1477, 1643
AfiI CCNNNNNNNGG 2 cut(s) 1301, 1516
AjuI GAANNNNNNNTTGG 4 cut(s) 1253, 1285, 1585, 1617
AloI GAACNNNNNNTCC 2 cut(s) 1657, 1689
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 2 cut(s) 346, 1299
Alw44I GTGCAC 1 cut(s) 551
AlwI GGATC 4 cut(s) 993, 1006, 1665, 1667
Ama87I CYCGRG 1 cut(s) 1300
ApaLI GTGCAC 1 cut(s) 551
ApeKI GCWGC 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 630
Asp718I GGTACC 1 cut(s) 435
AspLEI GCGC 1 cut(s) 424
AspS9I GGNCC 2 cut(s) 1621, 1754
AsuHPI GGTGA 3 cut(s) 72, 968, 1594
AsuII TTCGAA 2 cut(s) 220, 238
AvaI CYCGRG 1 cut(s) 1300
AvaII GGWCC 2 cut(s) 1621, 1754
BaeGI GKGCMC 1 cut(s) 555
BamHI GGATCC 1 cut(s) 998
BanI GGYRCC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 1 cut(s) 241
BccI CCATC 3 cut(s) 447, 880, 941
BcoDI GTCTC 2 cut(s) 346, 1299
BfaI CTAG 3 cut(s) 282, 683, 1749
BisI GCNGC 2 cut(s) 255, 1405
BlsI GCNGC 2 cut(s) 256, 1406
Bme18I GGWCC 2 cut(s) 1621, 1754
BmeT110I CYCGRG 1 cut(s) 1300
BmgT120I GGNCC 2 cut(s) 1621, 1754
BmiI GGNNCC 4 cut(s) 437, 851, 1000, 1622
BmsI GCATC 5 cut(s) 38, 292, 846, 1242, 1339
BoxI GACNNNNGTC 1 cut(s) 626
Bpu10I CCTNAGC 2 cut(s) 1628, 1688
Bpu14I TTCGAA 2 cut(s) 220, 238
BpuEI CTTGAG 2 cut(s) 1637, 1743
BsaBI GATNNNNATC 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 1676
Bsc4I CCNNNNNNNGG 2 cut(s) 1301, 1516
Bse1I ACTGG 1 cut(s) 1049
Bse3DI GCAATG 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 1676
BseGI GGATG 8 cut(s) 307, 439, 751, 861, 1233, 1354, 1474, 1744
BseJI GATNNNNATC 1 cut(s) 28
BseLI CCNNNNNNNGG 2 cut(s) 1301, 1516
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 5 cut(s) 34, 341, 483, 1383, 1722
BseNI ACTGG 1 cut(s) 1049
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 1 cut(s) 241
BseYI CCCAGC 1 cut(s) 1208
Bsh1236I CGCG 2 cut(s) 424, 1404
BshNI GGYRCC 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 555
BsiHKCI CYCGRG 1 cut(s) 1300
BsiSI CCGG 1 cut(s) 853
BslI CCNNNNNNNGG 2 cut(s) 1301, 1516
BsmAI GTCTC 2 cut(s) 346, 1299
BsmBI CGTCTC 1 cut(s) 1299
BsoBI CYCGRG 1 cut(s) 1300
Bsp119I TTCGAA 2 cut(s) 220, 238
Bsp1286I GDGCHC 1 cut(s) 555
Bsp1407I TGTACA 3 cut(s) 1226, 1475, 1641
Bsp143I GATC 5 cut(s) 77, 998, 1316, 1657, 1672
BspACI CCGC 6 cut(s) 248, 412, 424, 433, 503, 1404
BspCNI CTCAG 5 cut(s) 33, 342, 484, 1382, 1721
BspFNI CGCG 2 cut(s) 424, 1404
BspLI GGNNCC 4 cut(s) 437, 851, 1000, 1622
BspPI GGATC 4 cut(s) 993, 1006, 1665, 1667
BspT104I TTCGAA 2 cut(s) 220, 238
BspT107I GGYRCC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 124
BsrGI TGTACA 3 cut(s) 1226, 1475, 1641
BsrI ACTGG 1 cut(s) 1049
BssECI CCNNGG 1 cut(s) 1676
BssMI GATC 5 cut(s) 77, 998, 1316, 1657, 1672
Bst4CI ACNGT 5 cut(s) 71, 143, 565, 906, 1183
Bst6I CTCTTC 1 cut(s) 1524
BstAUI TGTACA 3 cut(s) 1226, 1475, 1641
BstBI TTCGAA 2 cut(s) 220, 238
BstC8I GCNNGC 4 cut(s) 414, 589, 828, 1013
BstEII GGTNACC 2 cut(s) 1562, 1582
BstF5I GGATG 8 cut(s) 307, 439, 751, 861, 1233, 1354, 1474, 1744
BstFNI CGCG 2 cut(s) 424, 1404
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 5 cut(s) 80, 1001, 1319, 1660, 1675
BstMAI GTCTC 2 cut(s) 346, 1299
BstMBI GATC 5 cut(s) 77, 998, 1316, 1657, 1672
BstMWI GCNNNNNNNGC 3 cut(s) 254, 430, 1394
BstNSI RCATGY 1 cut(s) 1233
BstPAI GACNNNNGTC 1 cut(s) 626
BstPI GGTNACC 2 cut(s) 1562, 1582
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 2 cut(s) 424, 1404
BstV1I GCAGC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 998
BstYI RGATCY 1 cut(s) 998
BtsCI GGATG 8 cut(s) 307, 439, 751, 861, 1233, 1354, 1474, 1744
BtsIMutI CAGTG 1 cut(s) 1388
Cac8I GCNNGC 4 cut(s) 414, 589, 828, 1013
CfoI GCGC 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 1621, 1754
CseI GACGC 1 cut(s) 973
Csp6I GTAC 9 cut(s) 139, 144, 279, 436, 566, 897, 1227, 1476, 1642
CspCI CAANNNNNGTGG 4 cut(s) 501, 536, 1236, 1271
CviAII CATG 4 cut(s) 275, 1108, 1230, 1661
CviQI GTAC 9 cut(s) 139, 144, 279, 436, 566, 897, 1227, 1476, 1642
DpnI GATC 5 cut(s) 79, 1000, 1318, 1659, 1674
DpnII GATC 5 cut(s) 77, 998, 1316, 1657, 1672
DraI TTTAAA 1 cut(s) 799
Eam1104I CTCTTC 1 cut(s) 1524
EarI CTCTTC 1 cut(s) 1524
Eco32I GATATC 2 cut(s) 1522, 1598
Eco47I GGWCC 2 cut(s) 1621, 1754
Eco57I CTGAAG 1 cut(s) 1406
Eco88I CYCGRG 1 cut(s) 1300
Eco91I GGTNACC 2 cut(s) 1562, 1582
EcoO65I GGTNACC 2 cut(s) 1562, 1582
EcoRI GAATTC 2 cut(s) 169, 1290
EcoRV GATATC 2 cut(s) 1522, 1598
EcoT22I ATGCAT 2 cut(s) 1085, 1235
Esp3I CGTCTC 1 cut(s) 1299
FaeI CATG 4 cut(s) 278, 1111, 1233, 1664
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FatI CATG 4 cut(s) 274, 1107, 1229, 1660
FauI CCCGC 1 cut(s) 405
FblI GTMKAC 1 cut(s) 1362
Fnu4HI GCNGC 2 cut(s) 255, 1405
FokI GGATG 8 cut(s) 314, 426, 758, 868, 1220, 1361, 1481, 1731
Fsp4HI GCNGC 2 cut(s) 255, 1405
FspBI CTAG 3 cut(s) 282, 683, 1749
GlaI GCGC 1 cut(s) 423
GluI GCNGC 2 cut(s) 255, 1405
GsaI CCCAGC 1 cut(s) 1212
HapII CCGG 1 cut(s) 853
HgaI GACGC 1 cut(s) 973
HhaI GCGC 1 cut(s) 424
Hin1II CATG 4 cut(s) 278, 1111, 1233, 1664
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HindIII AAGCTT 2 cut(s) 344, 669
HinfI GANTC 3 cut(s) 484, 1045, 1298
HpaII CCGG 1 cut(s) 853
HphI GGTGA 3 cut(s) 72, 968, 1594
Hpy166II GTNNAC 5 cut(s) 297, 553, 1190, 1227, 1363
Hpy188I TCNGA 8 cut(s) 23, 175, 184, 351, 399, 493, 1372, 1711
Hpy188III TCNNGA 7 cut(s) 338, 1093, 1237, 1295, 1302, 1424, 1463
Hpy8I GTNNAC 5 cut(s) 297, 553, 1190, 1227, 1363
HpyAV CCTTC 3 cut(s) 498, 809, 1597
HpyCH4III ACNGT 5 cut(s) 71, 143, 565, 906, 1183
HpyCH4IV ACGT 2 cut(s) 137, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 430, 1394
HpySE526I ACGT 2 cut(s) 137, 524
Hsp92II CATG 4 cut(s) 278, 1111, 1233, 1664
HspAI GCGC 1 cut(s) 422
KpnI GGTACC 1 cut(s) 439
Kzo9I GATC 5 cut(s) 77, 998, 1316, 1657, 1672
LmnI GCTCC 3 cut(s) 152, 849, 1417
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 5 cut(s) 38, 292, 846, 1242, 1339
MaeI CTAG 3 cut(s) 282, 683, 1749
MaeII ACGT 2 cut(s) 137, 524
MaeIII GTNAC 2 cut(s) 1562, 1582
MalI GATC 5 cut(s) 79, 1000, 1318, 1659, 1674
MboI GATC 5 cut(s) 77, 998, 1316, 1657, 1672
MfeI CAATTG 5 cut(s) 102, 417, 914, 1556, 1645
MflI RGATCY 1 cut(s) 998
MhlI GDGCHC 1 cut(s) 555
MlyI GAGTC 1 cut(s) 1292
MmeI TCCRAC 2 cut(s) 243, 1326
MnlI CCTC 8 cut(s) 278, 339, 640, 1459, 1506, 1686, 1762, 1767
Mph1103I ATGCAT 2 cut(s) 1085, 1235
MroXI GAANNNNTTC 1 cut(s) 630
MslI CAYNNNNRTG 1 cut(s) 1749
MspA1I CMGCKG 2 cut(s) 1015, 1670
MspI CCGG 1 cut(s) 853
MunI CAATTG 5 cut(s) 102, 417, 914, 1556, 1645
MvnI CGCG 2 cut(s) 424, 1404
MwoI GCNNNNNNNGC 3 cut(s) 254, 430, 1394
NdeII GATC 5 cut(s) 77, 998, 1316, 1657, 1672
NlaIII CATG 4 cut(s) 278, 1111, 1233, 1664
NlaIV GGNNCC 4 cut(s) 437, 851, 1000, 1622
NmeAIII GCCGAG 1 cut(s) 1657
NmuCI GTSAC 1 cut(s) 1582
NsiI ATGCAT 2 cut(s) 1085, 1235
NspI RCATGY 1 cut(s) 1233
NspV TTCGAA 2 cut(s) 220, 238
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 484, 1045
PkrI GCNGC 2 cut(s) 256, 1406
PleI GAGTC 1 cut(s) 1292
PpsI GAGTC 1 cut(s) 1292
PshAI GACNNNNGTC 1 cut(s) 626
PspEI GGTNACC 2 cut(s) 1562, 1582
PspFI CCCAGC 1 cut(s) 1208
PspN4I GGNNCC 4 cut(s) 437, 851, 1000, 1622
PspPI GGNCC 2 cut(s) 1621, 1754
PsrI GAACNNNNNNTAC 2 cut(s) 127, 159
PsuI RGATCY 1 cut(s) 998
PvuII CAGCTG 2 cut(s) 1015, 1670
RsaI GTAC 9 cut(s) 140, 145, 280, 437, 567, 898, 1228, 1477, 1643
RsaNI GTAC 9 cut(s) 139, 144, 279, 436, 566, 897, 1227, 1476, 1642
RseI CAYNNNNRTG 1 cut(s) 1749
SatI GCNGC 2 cut(s) 255, 1405
Sau3AI GATC 5 cut(s) 77, 998, 1316, 1657, 1672
Sau96I GGNCC 2 cut(s) 1621, 1754
SchI GAGTC 1 cut(s) 1292
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 5 cut(s) 38, 292, 846, 1242, 1339
SfuI TTCGAA 2 cut(s) 220, 238
SinI GGWCC 2 cut(s) 1621, 1754
SmiMI CAYNNNNRTG 1 cut(s) 1749
SmlI CTYRAG 2 cut(s) 1652, 1758
SmoI CTYRAG 2 cut(s) 1652, 1758
SsiI CCGC 6 cut(s) 248, 412, 424, 433, 503, 1404
SspI AATATT 2 cut(s) 1026, 1378
SspMI CTAG 3 cut(s) 282, 683, 1749
TaaI ACNGT 5 cut(s) 71, 143, 565, 906, 1183
TaiI ACGT 2 cut(s) 140, 527
TaqI TCGA 6 cut(s) 194, 220, 238, 339, 659, 1574
TatI WGTACW 4 cut(s) 565, 1226, 1475, 1641
TauI GCSGC 1 cut(s) 1407
TfiI GAWTC 2 cut(s) 484, 1045
TscAI CASTG 1 cut(s) 1395
TseFI GTSAC 1 cut(s) 1582
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 1582
TspDTI ATGAA 3 cut(s) 690, 1124, 1797
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 1 cut(s) 1395
VneI GTGCAC 1 cut(s) 551
VpaK11BI GGWCC 2 cut(s) 1621, 1754
XceI RCATGY 1 cut(s) 1233
XcmI CCANNNNNNNNNTGG 2 cut(s) 701, 1576
XmiI GTMKAC 1 cut(s) 1362
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 3 cut(s) 282, 683, 1749
Zsp2I ATGCAT 2 cut(s) 1085, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.