pycom14g04850
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001228
Physical Location & Seq
Forward (+)
4581758 .. 4583858
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04850.2

Sequence Viewer

Length: 1920 bp
ATGCCTGACTGTTCTTCAAATCCTAAGGGTTGGAATCTCTTCAAATTTGCCATGACCCATTTGCAGAAACTCAGATTAACATCTCTTCTCAACTGGGTTTCTTCGAATTTGTTCAAAAACCGTGTTGGATCATCACCAATCCCATTTCATCCGATTGGGTATGTCTGCAATGCCCCAATCTTTAGAATGTACCGAACCAATGGAGCTTCTGAAATTGAGGATTCTGAAAATTCGTATAAAGCTTCGAGTGGTAAGGGAAAAAATGCTGGACGAATTTCTTGTTCCATTCGCAAAGAAGCTGAAGATGCATTGTTGGATTATTTGCACGGTACTAGAGGGTTGCAGTTCACGGATGCAGAGAATATGAGTAAGAACTCGCCTCTGTTTCTGGACAAGCTTCTGAAACAGGTTGACATTGAAAAAGAAATTCCTGCCACAAAGAATAATCAGAAAGAAATTGGGCGAGCAATTGCGCGGTTCTTGCGTTACCACCCTATTAACGAATTTGAGCCTTTCTTTGAGAGCTTGGGCTTGAAGCCTTCTGAGTATTCTCCGCTTCTTCCACGCAGTTTGACGTTTTTGACCGATGATAAGTTATTGGTGCACAATTATACTGTACTATGTCATTATGGGATTGCACGCAGTAAGATAGGAAAGATTTACAAGGAATCGACAGAAGTCTTTCGATATGATTTTGAGGTTTTAACATCGAAACTTAAAGCTTATGAAGAACTAGGTCTTAGCCAATCTAGTTTTGTTAAGTTTATAGTTGCTACTCCTTGTCTTGTAATTGGGGATGTAAATGTTGAATTTGTTAAGGTATTGGAAAAATTACAGAGTTTTGGATTTGAAACCAATTGGATTGAGGGGAATCTGTCTACTGATAATTCTCATAATTTTCGCCGGATGCTTAAAGTTCTAAGTTTGTTTAGTGAGATGGGTTGCAGCAACGAACAGTTGGGTCAATTGATTGGCCAGCACCCAGATATTTTGTTTGATGGTTCGGGTGAAAGGACGCTTTCACTAATTGGGTTCTTTCTGAAATTTGGATCCACAAAAAGCCACCTATGCTCAATATTTCTGCAATTTCCACAGATTCCAGTTGTGAAATTTGTTTCAAATTTGAGACAATGCATTTTGTTCCTGAATGAAATTGACATGAAGGTTGCAGATATTGGGAAGTTTGTCCATACCCACACCCTGCTTCTTGGTTCTTGTTCTTTGAAAAAAACCGTCAGCTTACTCACTAACTTGAGCACCGGGAAGAAAAGATTATGTATATACATCCAGGAGAACCCACAAGAACTGAAGAATTGGGTTTTGGGTAAAAGAGTTCAGCCATTTCCAGACTCGGGAGATGACCTTAGATCAAAGACACAAAAGATTAAGTTTTTGTTTGATGTCGGATTTGTAGACAACTCAGACAATATCAAAGAAGCACTGAAAGCATTTCGAGGCAAGGGAGTGGAGCTTCAGGAAAGATTTGATTGTATTGTTAAAGCTGGTTTGAGTCCAGAGGATGTCTGTACAATGATTAAAATAAGCCCTCAAATTCTTAACCAGACAAAGGATATCATCGAAGAGAAGATCGATTTTCTTGTAAATCAATTGGGTTACCCCATATCGACTTTGGTGACCTTCCCAAGATATCTTTCCCATAAGATCGAAAGGGTCCAGCTTAGAATACTGATGTACAATTGGCTCAAAGATCATGGAACAGTTGATCCTCAAATGAAATTCATTCCAAGGATTAAGCTATTCATTGCCTTTTCTGTTATGGTTCTTTCTGCATTGTTCATTATTCTTAAGTTGATAGTTGATACTAACATACTTCTTCCTGTTTCTTTCACTTGCGGACATTTTCTTAGCGTTACAAAGCAAGAAGGCACATACGAGTTAAATACAGCAAAAAGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

640

Amino Acids

72.64

Weight (kDa)

8.96

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 878, 1413
AccII CGCG 1 cut(s) 475
AciI CCGC 3 cut(s) 475, 554, 1854
AclWI GGATC 4 cut(s) 136, 1044, 1057, 1718
AcoI YGGCCR 1 cut(s) 973
AcuI CTGAAG 3 cut(s) 321, 1328, 1457
AfaI GTAC 5 cut(s) 191, 331, 618, 1528, 1694
AfiI CCNNNNNNNGG 2 cut(s) 1352, 1567
AflII CTTAAG 1 cut(s) 1805
AgsI TTSAA 9 cut(s) 18, 43, 115, 419, 535, 809, 851, 1119, 1225
AjnI CCWGG 1 cut(s) 1287
AjuI GAANNNNNNNTTGG 4 cut(s) 1304, 1336, 1636, 1668
AloI GAACNNNNNNTCC 2 cut(s) 1708, 1740
Alw21I GWGCWC 2 cut(s) 606, 1259
Alw26I GTCTC 1 cut(s) 1120
Alw44I GTGCAC 1 cut(s) 602
AlwI GGATC 4 cut(s) 136, 1044, 1057, 1718
Ama87I CYCGRG 1 cut(s) 1351
AoxI GGCC 1 cut(s) 973
ApaLI GTGCAC 1 cut(s) 602
ApeKI GCWGC 1 cut(s) 945
Asp700I GAANNNNTTC 3 cut(s) 38, 110, 681
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 1 cut(s) 1672
AsuC2I CCSGG 1 cut(s) 1261
AsuHPI GGTGA 3 cut(s) 126, 1019, 1645
AsuII TTCGAA 1 cut(s) 104
AvaI CYCGRG 1 cut(s) 1351
AvaII GGWCC 1 cut(s) 1672
AxyI CCTNAGG 1 cut(s) 24
BaeGI GKGCMC 1 cut(s) 606
BalI TGGCCA 1 cut(s) 975
BamHI GGATCC 1 cut(s) 1049
Bbv12I GWGCWC 2 cut(s) 606, 1259
BbvI GCAGC 1 cut(s) 957
BccI CCATC 2 cut(s) 931, 992
BciT130I CCWGG 1 cut(s) 1289
BcnI CCSGG 1 cut(s) 1261
BcoDI GTCTC 1 cut(s) 1120
BfaI CTAG 3 cut(s) 333, 734, 750
BfrI CTTAAG 1 cut(s) 1805
BisI GCNGC 1 cut(s) 946
BlsI GCNGC 1 cut(s) 947
Bme1390I CCNGG 2 cut(s) 1261, 1289
Bme18I GGWCC 1 cut(s) 1672
BmeT110I CYCGRG 1 cut(s) 1351
BmgT120I GGNCC 1 cut(s) 1672
BmiI GGNNCC 2 cut(s) 1051, 1673
BmrFI CCNGG 2 cut(s) 1261, 1289
BmrI ACTGGG 1 cut(s) 103
BmsI GCATC 3 cut(s) 295, 343, 897
BmuI ACTGGG 1 cut(s) 103
BoxI GACNNNNGTC 1 cut(s) 677
Bpu14I TTCGAA 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 1273
BpuMI CCSGG 1 cut(s) 1261
Bsa29I ATCGAT 1 cut(s) 1590
BsaBI GATNNNNATC 1 cut(s) 79
BsaJI CCNNGG 1 cut(s) 1745
Bsc4I CCNNNNNNNGG 2 cut(s) 1352, 1567
Bse1I ACTGG 2 cut(s) 98, 1100
Bse21I CCTNAGG 1 cut(s) 24
Bse3DI GCAATG 2 cut(s) 175, 1761
Bse8I GATNNNNATC 1 cut(s) 79
BseBI CCWGG 1 cut(s) 1289
BseCI ATCGAT 1 cut(s) 1590
BseDI CCNNGG 1 cut(s) 1745
BseGI GGATG 6 cut(s) 148, 358, 802, 912, 1284, 1525
BseJI GATNNNNATC 1 cut(s) 79
BseLI CCNNNNNNNGG 2 cut(s) 1352, 1567
BseMI GCAATG 2 cut(s) 175, 1761
BseMII CTCAG 3 cut(s) 85, 534, 1434
BseNI ACTGG 2 cut(s) 98, 1100
BseSI GKGCMC 1 cut(s) 606
BseXI GCAGC 1 cut(s) 957
Bsh1236I CGCG 1 cut(s) 475
BshFI GGCC 1 cut(s) 975
BshVI ATCGAT 1 cut(s) 1590
BsiHKAI GWGCWC 2 cut(s) 606, 1259
BsiHKCI CYCGRG 1 cut(s) 1351
BsiSI CCGG 2 cut(s) 904, 1260
BslI CCNNNNNNNGG 2 cut(s) 1352, 1567
BsmAI GTCTC 1 cut(s) 1120
BsnI GGCC 1 cut(s) 975
BsoBI CYCGRG 1 cut(s) 1351
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 606, 1259
Bsp1407I TGTACA 2 cut(s) 1526, 1692
Bsp143I GATC 7 cut(s) 128, 1049, 1367, 1587, 1662, 1708, 1723
BspACI CCGC 3 cut(s) 475, 554, 1854
BspANI GGCC 1 cut(s) 975
BspCNI CTCAG 3 cut(s) 84, 535, 1433
BspDI ATCGAT 1 cut(s) 1590
BspFNI CGCG 1 cut(s) 475
BspLI GGNNCC 2 cut(s) 1051, 1673
BspPI GGATC 4 cut(s) 136, 1044, 1057, 1718
BspT104I TTCGAA 1 cut(s) 104
BspTI CTTAAG 1 cut(s) 1805
BsrDI GCAATG 2 cut(s) 175, 1761
BsrGI TGTACA 2 cut(s) 1526, 1692
BsrI ACTGG 2 cut(s) 98, 1100
BssECI CCNNGG 1 cut(s) 1745
BssMI GATC 7 cut(s) 128, 1049, 1367, 1587, 1662, 1708, 1723
BssT1I CCWWGG 1 cut(s) 1745
Bst2UI CCWGG 1 cut(s) 1289
Bst4CI ACNGT 7 cut(s) 11, 122, 329, 616, 957, 1234, 1720
Bst6I CTCTTC 3 cut(s) 44, 90, 1575
BstAFI CTTAAG 1 cut(s) 1805
BstAUI TGTACA 2 cut(s) 1526, 1692
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 3 cut(s) 465, 640, 977
BstDEI CTNAG 9 cut(s) 24, 71, 543, 740, 920, 1364, 1420, 1679, 1865
BstEII GGTNACC 2 cut(s) 1613, 1633
BstF5I GGATG 6 cut(s) 148, 358, 802, 912, 1284, 1525
BstFNI CGCG 1 cut(s) 475
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 7 cut(s) 131, 1052, 1370, 1590, 1665, 1711, 1726
BstMAI GTCTC 1 cut(s) 1120
BstMBI GATC 7 cut(s) 128, 1049, 1367, 1587, 1662, 1708, 1723
BstMWI GCNNNNNNNGC 4 cut(s) 305, 481, 1068, 1445
BstNI CCWGG 1 cut(s) 1289
BstPAI GACNNNNGTC 1 cut(s) 677
BstPI GGTNACC 2 cut(s) 1613, 1633
BstSCI CCNGG 2 cut(s) 1259, 1287
BstSLI GKGCMC 1 cut(s) 606
BstUI CGCG 1 cut(s) 475
BstV1I GCAGC 1 cut(s) 957
BstX2I RGATCY 1 cut(s) 1049
BstYI RGATCY 1 cut(s) 1049
Bsu15I ATCGAT 1 cut(s) 1590
Bsu36I CCTNAGG 1 cut(s) 24
BsuRI GGCC 1 cut(s) 975
BsuTUI ATCGAT 1 cut(s) 1590
BtsCI GGATG 6 cut(s) 148, 358, 802, 912, 1284, 1525
BtsIMutI CAGTG 1 cut(s) 1439
Cac8I GCNNGC 3 cut(s) 465, 640, 977
CfoI GCGC 1 cut(s) 475
Cfr13I GGNCC 1 cut(s) 1672
ClaI ATCGAT 1 cut(s) 1590
CseI GACGC 1 cut(s) 1024
Csp6I GTAC 5 cut(s) 190, 330, 617, 1527, 1693
CspCI CAANNNNNGTGG 2 cut(s) 552, 587
CviAII CATG 3 cut(s) 52, 1159, 1712
CviQI GTAC 5 cut(s) 190, 330, 617, 1527, 1693
DdeI CTNAG 9 cut(s) 24, 71, 543, 740, 920, 1364, 1420, 1679, 1865
DpnI GATC 7 cut(s) 130, 1051, 1369, 1589, 1664, 1710, 1725
DpnII GATC 7 cut(s) 128, 1049, 1367, 1587, 1662, 1708, 1723
EaeI YGGCCR 1 cut(s) 973
Eam1104I CTCTTC 3 cut(s) 44, 90, 1575
EarI CTCTTC 3 cut(s) 44, 90, 1575
Eco130I CCWWGG 1 cut(s) 1745
Eco32I GATATC 2 cut(s) 1573, 1649
Eco47I GGWCC 1 cut(s) 1672
Eco57I CTGAAG 3 cut(s) 321, 1328, 1457
Eco81I CCTNAGG 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 1351
Eco91I GGTNACC 2 cut(s) 1613, 1633
EcoO65I GGTNACC 2 cut(s) 1613, 1633
EcoRII CCWGG 1 cut(s) 1287
EcoRV GATATC 2 cut(s) 1573, 1649
EcoT14I CCWWGG 1 cut(s) 1745
EcoT22I ATGCAT 2 cut(s) 310, 1136
ErhI CCWWGG 1 cut(s) 1745
FaeI CATG 3 cut(s) 55, 1162, 1715
FatI CATG 3 cut(s) 51, 1158, 1711
FblI GTMKAC 2 cut(s) 878, 1413
Fnu4HI GCNGC 1 cut(s) 946
FokI GGATG 6 cut(s) 135, 365, 809, 919, 1271, 1532
Fsp4HI GCNGC 1 cut(s) 946
FspBI CTAG 3 cut(s) 333, 734, 750
GlaI GCGC 1 cut(s) 474
GluI GCNGC 1 cut(s) 946
HaeIII GGCC 1 cut(s) 975
HapII CCGG 2 cut(s) 904, 1260
HgaI GACGC 1 cut(s) 1024
HhaI GCGC 1 cut(s) 475
Hin1II CATG 3 cut(s) 55, 1162, 1715
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HindIII AAGCTT 3 cut(s) 240, 395, 720
HinfI GANTC 7 cut(s) 34, 221, 668, 871, 1096, 1349, 1510
HpaII CCGG 2 cut(s) 904, 1260
HphI GGTGA 3 cut(s) 126, 1019, 1645
Hpy166II GTNNAC 5 cut(s) 348, 412, 604, 879, 1414
Hpy188III TCNNGA 6 cut(s) 389, 1144, 1346, 1353, 1475, 1514
Hpy8I GTNNAC 5 cut(s) 348, 412, 604, 879, 1414
HpyAV CCTTC 4 cut(s) 549, 1156, 1648, 1877
HpyCH4III ACNGT 7 cut(s) 11, 122, 329, 616, 957, 1234, 1720
HpyCH4IV ACGT 1 cut(s) 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 305, 481, 1068, 1445
HpyF3I CTNAG 9 cut(s) 24, 71, 543, 740, 920, 1364, 1420, 1679, 1865
HpySE526I ACGT 1 cut(s) 575
Hsp92II CATG 3 cut(s) 55, 1162, 1715
HspAI GCGC 1 cut(s) 473
Kzo9I GATC 7 cut(s) 128, 1049, 1367, 1587, 1662, 1708, 1723
LmnI GCTCC 2 cut(s) 203, 1468
Lsp1109I GCAGC 1 cut(s) 957
LweI GCATC 3 cut(s) 295, 343, 897
MaeI CTAG 3 cut(s) 333, 734, 750
MaeII ACGT 1 cut(s) 575
MaeIII GTNAC 4 cut(s) 485, 1613, 1633, 1870
MalI GATC 7 cut(s) 130, 1051, 1369, 1589, 1664, 1710, 1725
MboI GATC 7 cut(s) 128, 1049, 1367, 1587, 1662, 1708, 1723
MfeI CAATTG 5 cut(s) 468, 856, 965, 1607, 1696
MflI RGATCY 1 cut(s) 1049
MhlI GDGCHC 2 cut(s) 606, 1259
MlsI TGGCCA 1 cut(s) 975
MluNI TGGCCA 1 cut(s) 975
MlyI GAGTC 2 cut(s) 1343, 1519
MmeI TCCRAC 4 cut(s) 11, 106, 294, 1384
MnlI CCTC 9 cut(s) 211, 329, 390, 691, 859, 1448, 1510, 1557, 1737
Mox20I TGGCCA 1 cut(s) 975
Mph1103I ATGCAT 2 cut(s) 310, 1136
MroXI GAANNNNTTC 3 cut(s) 38, 110, 681
MscI TGGCCA 1 cut(s) 975
Msp20I TGGCCA 1 cut(s) 975
MspCI CTTAAG 1 cut(s) 1805
MspI CCGG 2 cut(s) 904, 1260
MspR9I CCNGG 2 cut(s) 1261, 1289
MunI CAATTG 5 cut(s) 468, 856, 965, 1607, 1696
MvaI CCWGG 1 cut(s) 1289
MvnI CGCG 1 cut(s) 475
MwoI GCNNNNNNNGC 4 cut(s) 305, 481, 1068, 1445
NciI CCSGG 1 cut(s) 1261
NdeII GATC 7 cut(s) 128, 1049, 1367, 1587, 1662, 1708, 1723
NlaIII CATG 3 cut(s) 55, 1162, 1715
NlaIV GGNNCC 2 cut(s) 1051, 1673
NmuCI GTSAC 1 cut(s) 1633
NsiI ATGCAT 2 cut(s) 310, 1136
NspV TTCGAA 1 cut(s) 104
PdmI GAANNNNTTC 3 cut(s) 38, 110, 681
PfeI GAWTC 5 cut(s) 34, 221, 668, 871, 1096
PfoI TCCNGGA 1 cut(s) 1287
PkrI GCNGC 1 cut(s) 947
PleI GAGTC 2 cut(s) 1343, 1518
PpsI GAGTC 2 cut(s) 1343, 1518
PshAI GACNNNNGTC 1 cut(s) 677
Psp6I CCWGG 1 cut(s) 1287
PspEI GGTNACC 2 cut(s) 1613, 1633
PspGI CCWGG 1 cut(s) 1287
PspN4I GGNNCC 2 cut(s) 1051, 1673
PspPI GGNCC 1 cut(s) 1672
PsuI RGATCY 1 cut(s) 1049
RsaI GTAC 5 cut(s) 191, 331, 618, 1528, 1694
RsaNI GTAC 5 cut(s) 190, 330, 617, 1527, 1693
SatI GCNGC 1 cut(s) 946
Sau3AI GATC 7 cut(s) 128, 1049, 1367, 1587, 1662, 1708, 1723
Sau96I GGNCC 1 cut(s) 1672
SchI GAGTC 2 cut(s) 1343, 1519
ScrFI CCNGG 2 cut(s) 1261, 1289
SduI GDGCHC 2 cut(s) 606, 1259
SfaNI GCATC 3 cut(s) 295, 343, 897
SfuI TTCGAA 1 cut(s) 104
SinI GGWCC 1 cut(s) 1672
SmlI CTYRAG 2 cut(s) 1252, 1805
SmoI CTYRAG 2 cut(s) 1252, 1805
SsiI CCGC 3 cut(s) 475, 554, 1854
SspI AATATT 1 cut(s) 1077
SspMI CTAG 3 cut(s) 333, 734, 750
StyD4I CCNGG 2 cut(s) 1259, 1287
StyI CCWWGG 1 cut(s) 1745
TaaI ACNGT 7 cut(s) 11, 122, 329, 616, 957, 1234, 1720
TaiI ACGT 1 cut(s) 578
TaqII GACCGA 1 cut(s) 599
TatI WGTACW 3 cut(s) 616, 1526, 1692
TfiI GAWTC 5 cut(s) 34, 221, 668, 871, 1096
TscAI CASTG 1 cut(s) 1446
TseFI GTSAC 1 cut(s) 1633
TseI GCWGC 1 cut(s) 945
Tsp45I GTSAC 1 cut(s) 1633
TspDTI ATGAA 8 cut(s) 137, 741, 1164, 1175, 1729, 1748, 1750, 1786
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 1446
Vha464I CTTAAG 1 cut(s) 1805
VneI GTGCAC 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 1672
XcmI CCANNNNNNNNNTGG 1 cut(s) 1627
XmiI GTMKAC 2 cut(s) 878, 1413
XmnI GAANNNNTTC 3 cut(s) 38, 110, 681
XspI CTAG 3 cut(s) 333, 734, 750
Zsp2I ATGCAT 2 cut(s) 310, 1136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.