Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0000935

Physical Location & Seq

Genomic Coordinates Forward (+)

32204226 .. 32206038

Gene Structure 1813 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1147600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGTCCAGCTGTGAAACGGCCGTGCTCGAAGATCGTACAAGGCCCCTGGAGGATCGCATTCTCTGCGCTCTCAACGTCCGCATTCCCTTTTCTGGTAATGGTGGTGGAATTGAGGAGGTTAGAGTTGGAATTGTTGCTGTTTTTGATGGTCATAGTGGTAGTGAAGCTAGTGATATGGCTTCCAAGCAGTTACTGGAGTATTTCGCTCTGCATATGCATTTTCTTGTCAGTGCTTCGTATTCTGCTATGTTGGAGAATGCTGCCTCAAGAACAGCAAGATTGCATAATTCGGATGGCTTTCAAGATCCTCAAGATTCCGATATTACTAGGATCGAGCATTCATTGCAACCCCACTTTGATGCATTTTTCCAGTCGGACATTTTGAAAGAAGCGTTGTTGAGGGCAATTCATGATATTGATGCAAAATTTTCCATAGAAGCATCTGCAAAAAATATTAGTTCAGGTTCTACAGCCACGATTGTACTACTAGCTAATGGTCAAATTTTAGTTGCCAATATTGGAGACTCGAAGGCTTTTTTGTGCTCGGAAAAATCTATGTATCCTGCTGAGGCTGAAGCCACTTGTCCGAGGCTATACACTCAGGAGAGGCATAAATGTAATAATGGTCCTGTTTCACGTCCAAGAAACTATGAAATGTTTGAATTGGCCAACCCTGAGGTAATGACTTTTTCTGCCAAGGAATTGACAAGAGACCATCATCCAGGCAGAGATGATGAAAAGTTTCGGGTGGAAACTGCTGGTGGCTATGATCTTTCATGGGGTGGTGTGCCTCGTGTAAATCATATTTCACAAGCAATTGGTCGTGTCTCAATTAAAAGGTTTGGCGTTCTCTCTGCACCAGAGTTGACAGATTGGCAACCCCTGACTGTAAATGATACCTACCTTGTAGCTGCATCTGTTGGAGTTTTCAGGAAACTGAGCGTGCAGGATGTTTGTGACTTAATGTTGGCAGCACAGAGATATACTCCTGGGAGATCAGAGTTGTCTTCACCCTGTTCATATTCATTAGCTGACTGCATAGTAAATACTGCAATGGAAAAGGGGAGTGTGGGCGGTGTAGCAGCTGTAGTCGTCACATTGGTATCTACTGGTTTGCAGAAAGTTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C Pkinase

Protein Analysis

377

Amino Acids

40.94

Weight (kDa)

5.5

Isoelectric Point (pI)

43.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	115 - 185	1e-08	Protein phosphatase 2C
PP2C	PF00481	232 - 323	6.3e-12	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000935)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63320 AT3G63320 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340
fragaria_vesca	FvH4_2g23010 FvH4_2g23010 FvH4_2g23010 FvH4_2g23010 FvH4_2g23010
malus_domestica	MD00G1147300.v1.1 MD00G1147600.v1.1 MD00G1147700.v1.1 MD00G1147800.v1.1 MD03G1258500.v1.1 MD08G1204000.v1.1
prunus_persica	Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1
pyrus_communis	pycom03g20470 pycom08g17600 pycom08g17610 pycom08g17640 pycom08g17650 pycom08g17660
rosa_chinensis	RchiOBHm_Chr3g0479641 RchiOBHm_Chr6g0290051
rosa_laevigata	RLG00000012250 RLG00000023551 RLG00000023553 RLG00000023556 RLG00000029889
rosa_multiflora	Rmu_co8325477.1_g000001 Rmu_sc0001775.1_g000013 Rmu_sc0002902.1_g000039
rosa_roxburghii	Rroxscaffold_6G00402100 Rroxscaffold_6G00402120 Rroxscaffold_7G00177390
rosa_rugosa	Rorug03G0176900 Rorug03G0177000 Rorug06G0209000 Rorug06G0209100
rosa_samantha	Rh3AG227700 Rh3BG260800 Rh3BG261000 Rh3CG258100 Rh3DG254200 Rh6AG320000 Rh6BG327200 Rh6CG332900 Rh6DG318800
rosa_wichuraiana	Rw3G020380 Rw6G027610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	79, 1074
AclWI	GGATC	3 cut(s)	60, 299, 338
AcoI	YGGCCR	2 cut(s)	18, 666
AcsI	RAATTY	2 cut(s)	425, 501
AcuI	CTGAAG	1 cut(s)	594
AfaI	GTAC	2 cut(s)	37, 483
AfiI	CCNNNNNNNGG	1 cut(s)	92
AgsI	TTSAA	3 cut(s)	302, 385, 662
AjiI	CACGTC	1 cut(s)	638
AjnI	CCWGG	3 cut(s)	45, 721, 988
AluBI	AGCT	6 cut(s)	9, 167, 491, 911, 1031, 1085
AluI	AGCT	6 cut(s)	9, 167, 491, 911, 1031, 1085
Alw21I	GWGCWC	2 cut(s)	27, 545
Alw26I	GTCTC	3 cut(s)	516, 705, 832
AlwI	GGATC	3 cut(s)	60, 299, 338
AlwNI	CAGNNNCTG	1 cut(s)	193
AoxI	GGCC	3 cut(s)	18, 41, 666
ApeKI	GCWGC	4 cut(s)	260, 911, 971, 1082
ApoI	RAATTY	2 cut(s)	425, 501
Asp700I	GAANNNNTTC	1 cut(s)	741
AspLEI	GCGC	1 cut(s)	68
AspS9I	GGNCC	2 cut(s)	42, 626
AsuHPI	GGTGA	1 cut(s)	1002
AvaII	GGWCC	1 cut(s)	626
AxyI	CCTNAGG	1 cut(s)	675
BaeI	ACNNNNGTAYC	2 cut(s)	1086, 1119
BalI	TGGCCA	1 cut(s)	668
BauI	CACGAG	1 cut(s)	792
BbsI	GAAGAC	1 cut(s)	999
Bbv12I	GWGCWC	2 cut(s)	27, 545
BbvCI	CCTCAGC	1 cut(s)	567
BbvI	GCAGC	4 cut(s)	247, 898, 983, 1094
BccI	CCATC	3 cut(s)	140, 287, 723
BceAI	ACGGC	2 cut(s)	5, 33
BciT130I	CCWGG	3 cut(s)	47, 723, 990
BciVI	GTATCC	1 cut(s)	570
BcoDI	GTCTC	3 cut(s)	516, 705, 832
BfaI	CTAG	3 cut(s)	168, 327, 488
BfmI	CTRYAG	2 cut(s)	468, 1086
BfuI	GTATCC	1 cut(s)	570
BisI	GCNGC	4 cut(s)	261, 912, 972, 1083
BlsI	GCNGC	4 cut(s)	262, 913, 973, 1084
Bme1390I	CCNGG	3 cut(s)	47, 723, 990
Bme18I	GGWCC	1 cut(s)	626
BmgBI	CACGTC	1 cut(s)	638
BmgT120I	GGNCC	2 cut(s)	42, 626
BmiI	GGNNCC	1 cut(s)	44
BmrFI	CCNGG	3 cut(s)	47, 723, 990
BmsI	GCATC	4 cut(s)	349, 409, 449, 923
BpiI	GAAGAC	1 cut(s)	999
BplI	GAGNNNNNCTC	2 cut(s)	970, 1002
BpmI	CTGGAG	2 cut(s)	68, 215
Bpu10I	CCTNAGC	1 cut(s)	567
BpuEI	CTTGAG	2 cut(s)	250, 294
BsaI	GGTCTC	1 cut(s)	705
BsaJI	CCNNGG	4 cut(s)	45, 587, 696, 989
Bsc4I	CCNNNNNNNGG	1 cut(s)	92
Bse1I	ACTGG	3 cut(s)	198, 370, 1114
Bse21I	CCTNAGG	1 cut(s)	675
Bse3DI	GCAATG	2 cut(s)	341, 1059
BseBI	CCWGG	3 cut(s)	47, 723, 990
BseDI	CCNNGG	4 cut(s)	45, 587, 696, 989
BseGI	GGATG	3 cut(s)	298, 718, 955
BseLI	CCNNNNNNNGG	1 cut(s)	92
BseMI	GCAATG	2 cut(s)	341, 1059
BseMII	CTCAG	4 cut(s)	558, 614, 666, 929
BseNI	ACTGG	3 cut(s)	198, 370, 1114
BseRI	GAGGAG	1 cut(s)	128
BseX3I	CGGCCG	1 cut(s)	18
BseXI	GCAGC	4 cut(s)	247, 898, 983, 1094
BsgI	GTGCAG	2 cut(s)	840, 965
Bsh1285I	CGRYCG	1 cut(s)	21
BshFI	GGCC	3 cut(s)	20, 43, 668
BsiEI	CGRYCG	1 cut(s)	21
BsiHKAI	GWGCWC	2 cut(s)	27, 545
BslI	CCNNNNNNNGG	1 cut(s)	92
BsmAI	GTCTC	3 cut(s)	516, 705, 832
BsmI	GAATGC	4 cut(s)	57, 81, 262, 337
BsnI	GGCC	3 cut(s)	20, 43, 668
Bso31I	GGTCTC	1 cut(s)	705
Bsp1286I	GDGCHC	2 cut(s)	27, 545
Bsp143I	GATC	6 cut(s)	31, 52, 304, 330, 769, 995
BspACI	CCGC	2 cut(s)	79, 1074
BspANI	GGCC	3 cut(s)	20, 43, 668
BspCNI	CTCAG	4 cut(s)	559, 613, 667, 930
BspHI	TCATGA	1 cut(s)	409
BspLI	GGNNCC	1 cut(s)	44
BspPI	GGATC	3 cut(s)	60, 299, 338
BspTNI	GGTCTC	1 cut(s)	705
BsrDI	GCAATG	2 cut(s)	341, 1059
BsrI	ACTGG	3 cut(s)	198, 370, 1114
BssECI	CCNNGG	4 cut(s)	45, 587, 696, 989
BssMI	GATC	6 cut(s)	31, 52, 304, 330, 769, 995
BssSI	CACGAG	1 cut(s)	792
BssT1I	CCWWGG	1 cut(s)	696
Bst2BI	CACGAG	1 cut(s)	792
Bst2UI	CCWGG	3 cut(s)	47, 723, 990
Bst4CI	ACNGT	1 cut(s)	889
BstAPI	GCANNNNNTGC	2 cut(s)	63, 343
BstC8I	GCNNGC	1 cut(s)	944
BstDEI	CTNAG	4 cut(s)	567, 600, 675, 938
BstF5I	GGATG	3 cut(s)	298, 718, 955
BstHHI	GCGC	1 cut(s)	68
BstKTI	GATC	6 cut(s)	34, 55, 307, 333, 772, 998
BstMAI	GTCTC	3 cut(s)	516, 705, 832
BstMBI	GATC	6 cut(s)	31, 52, 304, 330, 769, 995
BstMCI	CGRYCG	1 cut(s)	21
BstMWI	GCNNNNNNNGC	2 cut(s)	63, 343
BstNI	CCWGG	3 cut(s)	47, 723, 990
BstSCI	CCNGG	3 cut(s)	45, 721, 988
BstSFI	CTRYAG	2 cut(s)	468, 1086
BstV1I	GCAGC	4 cut(s)	247, 898, 983, 1094
BstV2I	GAAGAC	1 cut(s)	999
BstX2I	RGATCY	1 cut(s)	304
BstYI	RGATCY	1 cut(s)	304
BstZI	CGGCCG	1 cut(s)	18
Bsu36I	CCTNAGG	1 cut(s)	675
BsuI	GTATCC	1 cut(s)	570
BsuRI	GGCC	3 cut(s)	20, 43, 668
BtrI	CACGTC	1 cut(s)	638
BtsCI	GGATG	3 cut(s)	298, 718, 955
BtsIMutI	CAGTG	1 cut(s)	235
Cac8I	GCNNGC	1 cut(s)	944
CaiI	CAGNNNCTG	1 cut(s)	193
CciI	TCATGA	1 cut(s)	409
CfoI	GCGC	1 cut(s)	68
Cfr13I	GGNCC	2 cut(s)	42, 626
Csp6I	GTAC	2 cut(s)	36, 482
CviAII	CATG	2 cut(s)	410, 777
CviQI	GTAC	2 cut(s)	36, 482
DdeI	CTNAG	4 cut(s)	567, 600, 675, 938
DpnI	GATC	6 cut(s)	33, 54, 306, 332, 771, 997
DpnII	GATC	6 cut(s)	31, 52, 304, 330, 769, 995
EaeI	YGGCCR	2 cut(s)	18, 666
EagI	CGGCCG	1 cut(s)	18
EclXI	CGGCCG	1 cut(s)	18
Eco130I	CCWWGG	1 cut(s)	696
Eco31I	GGTCTC	1 cut(s)	705
Eco47I	GGWCC	1 cut(s)	626
Eco52I	CGGCCG	1 cut(s)	18
Eco57I	CTGAAG	1 cut(s)	594
Eco81I	CCTNAGG	1 cut(s)	675
EcoO109I	RGGNCCY	1 cut(s)	42
EcoRII	CCWGG	3 cut(s)	45, 721, 988
EcoT14I	CCWWGG	1 cut(s)	696
EcoT22I	ATGCAT	2 cut(s)	219, 364
ErhI	CCWWGG	1 cut(s)	696
FaeI	CATG	2 cut(s)	413, 780
FatI	CATG	2 cut(s)	409, 776
FauNDI	CATATG	1 cut(s)	213
Fnu4HI	GCNGC	4 cut(s)	261, 912, 972, 1083
FokI	GGATG	3 cut(s)	305, 705, 962
Fsp4HI	GCNGC	4 cut(s)	261, 912, 972, 1083
FspBI	CTAG	3 cut(s)	168, 327, 488
GlaI	GCGC	1 cut(s)	67
GluI	GCNGC	4 cut(s)	261, 912, 972, 1083
GsuI	CTGGAG	2 cut(s)	68, 215
HaeIII	GGCC	3 cut(s)	20, 43, 668
HhaI	GCGC	1 cut(s)	68
Hin1II	CATG	2 cut(s)	413, 780
Hin6I	GCGC	1 cut(s)	66
HinP1I	GCGC	1 cut(s)	66
HincII	GTYRAC	1 cut(s)	867
HindII	GTYRAC	1 cut(s)	867
HinfI	GANTC	2 cut(s)	314, 524
HphI	GGTGA	1 cut(s)	1002
Hpy166II	GTNNAC	1 cut(s)	867
Hpy188I	TCNGA	6 cut(s)	292, 319, 376, 547, 588, 1000
Hpy188III	TCNNGA	6 cut(s)	267, 302, 311, 410, 602, 931
Hpy8I	GTNNAC	1 cut(s)	867
HpyAV	CCTTC	1 cut(s)	523
HpyCH4III	ACNGT	1 cut(s)	889
HpyCH4IV	ACGT	2 cut(s)	75, 637
HpyF10VI	GCNNNNNNNGC	2 cut(s)	63, 343
HpyF3I	CTNAG	4 cut(s)	567, 600, 675, 938
HpySE526I	ACGT	2 cut(s)	75, 637
Hsp92II	CATG	2 cut(s)	413, 780
HspAI	GCGC	1 cut(s)	66
Kzo9I	GATC	6 cut(s)	31, 52, 304, 330, 769, 995
Lsp1109I	GCAGC	4 cut(s)	247, 898, 983, 1094
LweI	GCATC	4 cut(s)	349, 409, 449, 923
MaeI	CTAG	3 cut(s)	168, 327, 488
MaeII	ACGT	2 cut(s)	75, 637
MaeIII	GTNAC	3 cut(s)	189, 956, 1093
MalI	GATC	6 cut(s)	33, 54, 306, 332, 771, 997
MboI	GATC	6 cut(s)	31, 52, 304, 330, 769, 995
MboII	GAAGA	2 cut(s)	41, 999
MfeI	CAATTG	1 cut(s)	816
MflI	RGATCY	1 cut(s)	304
MhlI	GDGCHC	2 cut(s)	27, 545
MlsI	TGGCCA	1 cut(s)	668
MluNI	TGGCCA	1 cut(s)	668
MlyI	GAGTC	1 cut(s)	518
MmeI	TCCRAC	4 cut(s)	106, 231, 354, 901
Mox20I	TGGCCA	1 cut(s)	668
Mph1103I	ATGCAT	2 cut(s)	219, 364
MroXI	GAANNNNTTC	1 cut(s)	741
MscI	TGGCCA	1 cut(s)	668
MseI	TTAA	2 cut(s)	834, 962
MslI	CAYNNNNRTG	1 cut(s)	357
Msp20I	TGGCCA	1 cut(s)	668
MspA1I	CMGCKG	2 cut(s)	9, 1085
MspR9I	CCNGG	3 cut(s)	47, 723, 990
MunI	CAATTG	1 cut(s)	816
Mva1269I	GAATGC	4 cut(s)	57, 81, 262, 337
MvaI	CCWGG	3 cut(s)	47, 723, 990
MwoI	GCNNNNNNNGC	2 cut(s)	63, 343
NdeI	CATATG	1 cut(s)	213
NdeII	GATC	6 cut(s)	31, 52, 304, 330, 769, 995
NlaIII	CATG	2 cut(s)	413, 780
NlaIV	GGNNCC	1 cut(s)	44
NmuCI	GTSAC	2 cut(s)	956, 1093
NsiI	ATGCAT	2 cut(s)	219, 364
PagI	TCATGA	1 cut(s)	409
PctI	GAATGC	4 cut(s)	57, 81, 262, 337
PdmI	GAANNNNTTC	1 cut(s)	741
PfeI	GAWTC	1 cut(s)	314
PkrI	GCNGC	4 cut(s)	262, 913, 973, 1084
PleI	GAGTC	1 cut(s)	518
PpsI	GAGTC	1 cut(s)	518
Psp6I	CCWGG	3 cut(s)	45, 721, 988
PspGI	CCWGG	3 cut(s)	45, 721, 988
PspN4I	GGNNCC	1 cut(s)	44
PspPI	GGNCC	2 cut(s)	42, 626
PstNI	CAGNNNCTG	1 cut(s)	193
PsuI	RGATCY	1 cut(s)	304
PvuII	CAGCTG	2 cut(s)	9, 1085
RsaI	GTAC	2 cut(s)	37, 483
RsaNI	GTAC	2 cut(s)	36, 482
RseI	CAYNNNNRTG	1 cut(s)	357
SaqAI	TTAA	2 cut(s)	834, 962
SatI	GCNGC	4 cut(s)	261, 912, 972, 1083
Sau3AI	GATC	6 cut(s)	31, 52, 304, 330, 769, 995
Sau96I	GGNCC	2 cut(s)	42, 626
SchI	GAGTC	1 cut(s)	518
ScrFI	CCNGG	3 cut(s)	47, 723, 990
SduI	GDGCHC	2 cut(s)	27, 545
SfaNI	GCATC	4 cut(s)	349, 409, 449, 923
SfcI	CTRYAG	2 cut(s)	468, 1086
SinI	GGWCC	1 cut(s)	626
SmiMI	CAYNNNNRTG	1 cut(s)	357
SmlI	CTYRAG	2 cut(s)	265, 309
SmoI	CTYRAG	2 cut(s)	265, 309
SsiI	CCGC	2 cut(s)	79, 1074
SspI	AATATT	2 cut(s)	454, 517
SspMI	CTAG	3 cut(s)	168, 327, 488
StyD4I	CCNGG	3 cut(s)	45, 721, 988
StyI	CCWWGG	1 cut(s)	696
TaaI	ACNGT	1 cut(s)	889
TaiI	ACGT	2 cut(s)	78, 640
TaqI	TCGA	3 cut(s)	27, 333, 527
TatI	WGTACW	1 cut(s)	481
TfiI	GAWTC	1 cut(s)	314
Tru1I	TTAA	2 cut(s)	834, 962
Tru9I	TTAA	2 cut(s)	834, 962
TscAI	CASTG	1 cut(s)	235
TseFI	GTSAC	2 cut(s)	956, 1093
TseI	GCWGC	4 cut(s)	260, 911, 971, 1082
Tsp45I	GTSAC	2 cut(s)	956, 1093
TspDTI	ATGAA	7 cut(s)	330, 398, 666, 750, 765, 1008, 1014
TspRI	CASTG	1 cut(s)	235
VpaK11BI	GGWCC	1 cut(s)	626
XapI	RAATTY	2 cut(s)	425, 501
XcmI	CCANNNNNNNNNTGG	1 cut(s)	190
XmnI	GAANNNNTTC	1 cut(s)	741
XspI	CTAG	3 cut(s)	168, 327, 488
Zsp2I	ATGCAT	2 cut(s)	219, 364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.