Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0000935

Physical Location & Seq

Genomic Coordinates Reverse (-)

25157110 .. 25159229

Gene Structure 2120 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023551

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1428 bp

ATGGAAGAACACAGCTCCTCCGGAGTTCGCCTAATCAGTTTTTCCTTAATCCTAGCCCTAATTACGACGACGACAGCCTCTTACGACAAGTCGTCGTCAACGTGTTTGACGCTTTACGAACAAGGCGGTGCTCCCGCGGTTTTCCAATCCCCAAAATGCCCTCGCTGGAAGCGCGCCGATTCCCTCCGGCGTTGCCAGACCGCCGTGCGTCAAGGTCGGAGAAACCACCTCGAGGATCGTGCTCTTTGCGCTCTCGACCTTCGCATTCCCTTTCCTGGTGAATTAGGGATTAAGGAGGTTAAAGTTGGGATTGTGGCTGTTTTTGATGGGCATAATGGTGCTGAAGCAAGTGATATGGCTTCAAAGCTTTTATTGGAGTATTTCGTTTTGCATACCTATTTTCTTCTTGATTATTCTGATGCATCGAATAAATATTCAAGAACAGCAATATTGCAAACTGGTAGAGAACTTTATACTTCAAATGGCGTATTCCGATTGGATATTTCAAGGTTTAATCCTTCTTTGAAAGCAAAGTTTGATGATTCGCGTCATTTTGATATTTTAAAAGAAGCATTGTTGAGAGCAGTTCGTGATATTGATGCAAAATTTTCTATGGAAGCATCTTGGAAAAATCTTGTATCTGGTACTACAGCCTCGATTATATTACTTGCTGATGGTCATATTTTAGTTGCCAATATTGGAGACTCAAAGGCTTTTCTGTGCTCTGAAAAATTTCAGTCTCCTGCAGAGGCTAAAGCCACTTATTTGAGATACAGGCGGGAAAGGCGTAATGGTGCCATATCATCTCCCTTAGGAAACTATAAGAAAAACTTTGAATTGGCTTCCTCCTCCTCTGGGTTGGTGCAGCTTTCTGTTAAGGAATTGACGAGAGATCATCATCCAGACAGAGATGATGAAAAATTCCGCGTGGAAACTGCTGGTGGCTATGTGGTATTGGGTGGTGTGCCTCGAGTAAATGGTCAGCTGGCTATTTCCAGATCTATTGGCGATATTTCCTTCAAAAGTTTCGGCGTTATATCTACACCAGAGTTGACCGACTGGCAACCCCTGAGTGCAAACGATACCTATCTGGTTGCTGCATCTGATGGAGTTTTTGAGAAGCAGAGTCTGCAGGATGTTTGTGATTTATTGTGGGAAGCTCGGAGTTATGATTCTAGGAGATCGGAGTTCTCTTCCTCGCCGTGTTCATACTCATTAGCCGACTGCATCGTGAATGCTGCACTCGACAAGGGCAGCATGGATAATGTGGCAGCTGTTGTCGTTCCAATGATATCTACTTGGTTTTTTGAAAGTTTTTTCATGGAAAGATCAGTTTCAGAGAGGGACAGAAACTGTGAAGCATTAGGATTAGGACTACAAAGTTCCACTGATGAGTGCACAGCTAATGACTTCAATTATGAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C Pkinase

Protein Analysis

476

Amino Acids

52.68

Weight (kDa)

5.86

Isoelectric Point (pI)

51.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	184 - 243	2.6e-06	Protein phosphatase 2C
PP2C	PF00481	293 - 423	1.3e-23	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000935)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63320 AT3G63320 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340
fragaria_vesca	FvH4_2g23010 FvH4_2g23010 FvH4_2g23010 FvH4_2g23010 FvH4_2g23010
malus_domestica	MD00G1147300.v1.1 MD00G1147600.v1.1 MD00G1147700.v1.1 MD00G1147800.v1.1 MD03G1258500.v1.1 MD08G1204000.v1.1
prunus_persica	Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1
pyrus_communis	pycom03g20470 pycom08g17600 pycom08g17610 pycom08g17640 pycom08g17650 pycom08g17660
rosa_chinensis	RchiOBHm_Chr3g0479641 RchiOBHm_Chr6g0290051
rosa_laevigata	RLG00000012250 RLG00000023551 RLG00000023553 RLG00000023556 RLG00000029889
rosa_multiflora	Rmu_co8325477.1_g000001 Rmu_sc0001775.1_g000013 Rmu_sc0002902.1_g000039
rosa_roxburghii	Rroxscaffold_6G00402100 Rroxscaffold_6G00402120 Rroxscaffold_7G00177390
rosa_rugosa	Rorug03G0176900 Rorug03G0177000 Rorug06G0209000 Rorug06G0209100
rosa_samantha	Rh3AG227700 Rh3BG260800 Rh3BG261000 Rh3CG258100 Rh3DG254200 Rh6AG320000 Rh6BG327200 Rh6CG332900 Rh6DG318800
rosa_wichuraiana	Rw3G020380 Rw6G027610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	230
AccB1I	GGYRCC	1 cut(s)	794
AccII	CGCG	4 cut(s)	137, 174, 547, 927
AccIII	TCCGGA	1 cut(s)	20
AciI	CCGC	6 cut(s)	126, 135, 137, 201, 778, 925
AclWI	GGATC	1 cut(s)	243
AcsI	RAATTY	3 cut(s)	605, 731, 920
AcuI	CTGAAG	1 cut(s)	363
AfaI	GTAC	1 cut(s)	646
AfiI	CCNNNNNNNGG	3 cut(s)	232, 275, 855
AflIII	ACRYGT	1 cut(s)	101
AgsI	TTSAA	9 cut(s)	363, 438, 480, 507, 526, 836, 1021, 1310, 1414
AhdI	GACNNNNNGTC	1 cut(s)	91
AjnI	CCWGG	1 cut(s)	274
AluBI	AGCT	7 cut(s)	15, 367, 868, 985, 1160, 1274, 1403
AluI	AGCT	7 cut(s)	15, 367, 868, 985, 1160, 1274, 1403
Alw21I	GWGCWC	4 cut(s)	133, 244, 725, 1400
Alw26I	GTCTC	2 cut(s)	696, 744
Alw44I	GTGCAC	1 cut(s)	1396
AlwI	GGATC	1 cut(s)	243
AlwNI	CAGNNNCTG	2 cut(s)	1129, 1353
Ama87I	CYCGRG	2 cut(s)	230, 969
Aor13HI	TCCGGA	1 cut(s)	20
ApaLI	GTGCAC	1 cut(s)	1396
ApeKI	GCWGC	5 cut(s)	865, 1097, 1238, 1254, 1271
ApoI	RAATTY	3 cut(s)	605, 731, 920
Asp700I	GAANNNNTTC	1 cut(s)	732
AspLEI	GCGC	3 cut(s)	174, 176, 251
AsuHPI	GGTGA	1 cut(s)	290
AvaI	CYCGRG	2 cut(s)	230, 969
AxyI	CCTNAGG	1 cut(s)	811
BaeGI	GKGCMC	1 cut(s)	1400
BanI	GGYRCC	1 cut(s)	794
Bbv12I	GWGCWC	4 cut(s)	133, 244, 725, 1400
BbvI	GCAGC	5 cut(s)	877, 1084, 1225, 1266, 1283
BccI	CCATC	3 cut(s)	320, 668, 1100
BceAI	ACGGC	2 cut(s)	188, 1186
BcgI	CGANNNNNNTGC	2 cut(s)	221, 255
BciT130I	CCWGG	1 cut(s)	276
BcoDI	GTCTC	2 cut(s)	696, 744
BfaI	CTAG	2 cut(s)	53, 1176
BfmI	CTRYAG	3 cut(s)	648, 744, 1130
BglII	AGATCT	1 cut(s)	998
BisI	GCNGC	5 cut(s)	866, 1098, 1239, 1255, 1272
BlsI	GCNGC	5 cut(s)	867, 1099, 1240, 1256, 1273
Bme1390I	CCNGG	1 cut(s)	276
BmeRI	GACNNNNNGTC	1 cut(s)	91
BmeT110I	CYCGRG	2 cut(s)	230, 969
BmiI	GGNNCC	1 cut(s)	796
BmrFI	CCNGG	1 cut(s)	276
BmsI	GCATC	6 cut(s)	409, 431, 589, 629, 1109, 1236
BsaBI	GATNNNNATC	2 cut(s)	897, 1086
BsaJI	CCNNGG	1 cut(s)	135
BsaWI	WCCGGW	1 cut(s)	20
BsaXI	ACNNNNNCTCC	1 cut(s)	32
Bsc4I	CCNNNNNNNGG	3 cut(s)	232, 275, 855
Bse1I	ACTGG	2 cut(s)	463, 1064
Bse21I	CCTNAGG	1 cut(s)	811
Bse8I	GATNNNNATC	2 cut(s)	897, 1086
BseAI	TCCGGA	1 cut(s)	20
BseBI	CCWGG	1 cut(s)	276
BseDI	CCNNGG	1 cut(s)	135
BseGI	GGATG	2 cut(s)	898, 1141
BseJI	GATNNNNATC	2 cut(s)	897, 1086
BseLI	CCNNNNNNNGG	3 cut(s)	232, 275, 855
BseMII	CTCAG	1 cut(s)	1061
BseNI	ACTGG	2 cut(s)	463, 1064
BsePI	GCGCGC	1 cut(s)	172
BseRI	GAGGAG	3 cut(s)	7, 838, 841
BseSI	GKGCMC	1 cut(s)	1400
BseXI	GCAGC	5 cut(s)	877, 1084, 1225, 1266, 1283
BsgI	GTGCAG	2 cut(s)	884, 1224
Bsh1236I	CGCG	4 cut(s)	137, 174, 547, 927
BshNI	GGYRCC	1 cut(s)	794
BsiHKAI	GWGCWC	4 cut(s)	133, 244, 725, 1400
BsiHKCI	CYCGRG	2 cut(s)	230, 969
BsiSI	CCGG	2 cut(s)	21, 187
BslFI	GGGAC	1 cut(s)	1358
BslI	CCNNNNNNNGG	3 cut(s)	232, 275, 855
BsmAI	GTCTC	2 cut(s)	696, 744
BsmFI	GGGAC	1 cut(s)	1358
BsmI	GAATGC	2 cut(s)	264, 1240
BsoBI	CYCGRG	2 cut(s)	230, 969
Bsp1286I	GDGCHC	4 cut(s)	133, 244, 725, 1400
Bsp13I	TCCGGA	1 cut(s)	20
Bsp143I	GATC	5 cut(s)	235, 892, 998, 1181, 1328
BspACI	CCGC	6 cut(s)	126, 135, 137, 201, 778, 925
BspCNI	CTCAG	1 cut(s)	1062
BspEI	TCCGGA	1 cut(s)	20
BspFNI	CGCG	4 cut(s)	137, 174, 547, 927
BspLI	GGNNCC	1 cut(s)	796
BspMAI	CTGCAG	2 cut(s)	748, 1134
BspPI	GGATC	1 cut(s)	243
BspT107I	GGYRCC	1 cut(s)	794
BsrI	ACTGG	2 cut(s)	463, 1064
BssECI	CCNNGG	1 cut(s)	135
BssHII	GCGCGC	1 cut(s)	172
BssMI	GATC	5 cut(s)	235, 892, 998, 1181, 1328
Bst2UI	CCWGG	1 cut(s)	276
Bst4CI	ACNGT	1 cut(s)	1355
Bst6I	CTCTTC	1 cut(s)	1198
BstAPI	GCANNNNNTGC	1 cut(s)	1129
BstC8I	GCNNGC	2 cut(s)	174, 987
BstDEI	CTNAG	2 cut(s)	811, 1070
BstDSI	CCRYGG	1 cut(s)	135
BstF5I	GGATG	2 cut(s)	898, 1141
BstFNI	CGCG	4 cut(s)	137, 174, 547, 927
BstHHI	GCGC	3 cut(s)	174, 176, 251
BstKTI	GATC	5 cut(s)	238, 895, 1001, 1184, 1331
BstMAI	GTCTC	2 cut(s)	696, 744
BstMBI	GATC	5 cut(s)	235, 892, 998, 1181, 1328
BstMWI	GCNNNNNNNGC	4 cut(s)	171, 248, 784, 1129
BstNI	CCWGG	1 cut(s)	276
BstSCI	CCNGG	1 cut(s)	274
BstSFI	CTRYAG	3 cut(s)	648, 744, 1130
BstSLI	GKGCMC	1 cut(s)	1400
BstUI	CGCG	4 cut(s)	137, 174, 547, 927
BstV1I	GCAGC	5 cut(s)	877, 1084, 1225, 1266, 1283
BstX2I	RGATCY	1 cut(s)	998
BstYI	RGATCY	1 cut(s)	998
Bsu36I	CCTNAGG	1 cut(s)	811
BtgI	CCRYGG	1 cut(s)	135
BtsCI	GGATG	2 cut(s)	898, 1141
BtsIMutI	CAGTG	1 cut(s)	1386
Cac8I	GCNNGC	2 cut(s)	174, 987
CaiI	CAGNNNCTG	2 cut(s)	1129, 1353
CfoI	GCGC	3 cut(s)	174, 176, 251
Cfr42I	CCGCGG	1 cut(s)	138
CseI	GACGC	3 cut(s)	118, 197, 536
Csp6I	GTAC	1 cut(s)	645
CviAII	CATG	2 cut(s)	1258, 1321
CviQI	GTAC	1 cut(s)	645
DdeI	CTNAG	2 cut(s)	811, 1070
DpnI	GATC	5 cut(s)	237, 894, 1000, 1183, 1330
DpnII	GATC	5 cut(s)	235, 892, 998, 1181, 1328
DraI	TTTAAA	1 cut(s)	564
DriI	GACNNNNNGTC	1 cut(s)	91
Eam1104I	CTCTTC	1 cut(s)	1198
Eam1105I	GACNNNNNGTC	1 cut(s)	91
EarI	CTCTTC	1 cut(s)	1198
Eco32I	GATATC	1 cut(s)	1293
Eco57I	CTGAAG	1 cut(s)	363
Eco81I	CCTNAGG	1 cut(s)	811
Eco88I	CYCGRG	2 cut(s)	230, 969
EcoRII	CCWGG	1 cut(s)	274
EcoRV	GATATC	1 cut(s)	1293
EcoT22I	ATGCAT	1 cut(s)	424
FaeI	CATG	2 cut(s)	1261, 1324
FalI	AAGNNNNNCTT	2 cut(s)	815, 847
FaqI	GGGAC	1 cut(s)	1358
FatI	CATG	2 cut(s)	1257, 1320
FauI	CCCGC	2 cut(s)	142, 771
Fnu4HI	GCNGC	5 cut(s)	866, 1098, 1239, 1255, 1272
FokI	GGATG	2 cut(s)	885, 1148
Fsp4HI	GCNGC	5 cut(s)	866, 1098, 1239, 1255, 1272
FspBI	CTAG	2 cut(s)	53, 1176
GlaI	GCGC	3 cut(s)	173, 175, 250
GluI	GCNGC	5 cut(s)	866, 1098, 1239, 1255, 1272
HapII	CCGG	2 cut(s)	21, 187
HgaI	GACGC	3 cut(s)	118, 197, 536
HhaI	GCGC	3 cut(s)	174, 176, 251
Hin1II	CATG	2 cut(s)	1261, 1324
Hin6I	GCGC	3 cut(s)	172, 174, 249
HinP1I	GCGC	3 cut(s)	172, 174, 249
HincII	GTYRAC	2 cut(s)	99, 1053
HindII	GTYRAC	2 cut(s)	99, 1053
HindIII	AAGCTT	1 cut(s)	365
HinfI	GANTC	5 cut(s)	179, 542, 704, 1126, 1172
HpaII	CCGG	2 cut(s)	21, 187
HphI	GGTGA	1 cut(s)	290
Hpy166II	GTNNAC	3 cut(s)	99, 1053, 1398
Hpy188I	TCNGA	8 cut(s)	219, 418, 494, 727, 1105, 1164, 1186, 1339
Hpy188III	TCNNGA	8 cut(s)	21, 254, 407, 438, 590, 902, 996, 1231
Hpy8I	GTNNAC	3 cut(s)	99, 1053, 1398
Hpy99I	CGWCG	3 cut(s)	70, 73, 97
HpyAV	CCTTC	3 cut(s)	269, 528, 1027
HpyCH4III	ACNGT	1 cut(s)	1355
HpyCH4IV	ACGT	1 cut(s)	101
HpyF10VI	GCNNNNNNNGC	4 cut(s)	171, 248, 784, 1129
HpyF3I	CTNAG	2 cut(s)	811, 1070
HpySE526I	ACGT	1 cut(s)	101
Hsp92II	CATG	2 cut(s)	1261, 1324
HspAI	GCGC	3 cut(s)	172, 174, 249
Kpn2I	TCCGGA	1 cut(s)	20
KspI	CCGCGG	1 cut(s)	138
Kzo9I	GATC	5 cut(s)	235, 892, 998, 1181, 1328
LmnI	GCTCC	2 cut(s)	20, 136
Lsp1109I	GCAGC	5 cut(s)	877, 1084, 1225, 1266, 1283
LweI	GCATC	6 cut(s)	409, 431, 589, 629, 1109, 1236
MaeI	CTAG	2 cut(s)	53, 1176
MaeII	ACGT	1 cut(s)	101
MalI	GATC	5 cut(s)	237, 894, 1000, 1183, 1330
MboI	GATC	5 cut(s)	235, 892, 998, 1181, 1328
MboII	GAAGA	3 cut(s)	17, 395, 1185
MflI	RGATCY	1 cut(s)	998
MhlI	GDGCHC	4 cut(s)	133, 244, 725, 1400
MluCI	AATT	8 cut(s)	60, 281, 605, 731, 836, 881, 920, 1414
MlyI	GAGTC	2 cut(s)	698, 1135
MmeI	TCCRAC	1 cut(s)	197
Mph1103I	ATGCAT	1 cut(s)	424
MroI	TCCGGA	1 cut(s)	20
MroXI	GAANNNNTTC	1 cut(s)	732
MseI	TTAA	6 cut(s)	47, 291, 300, 513, 563, 876
MslI	CAYNNNNRTG	1 cut(s)	336
MspA1I	CMGCKG	3 cut(s)	137, 985, 1274
MspI	CCGG	2 cut(s)	21, 187
MspR9I	CCNGG	1 cut(s)	276
Mva1269I	GAATGC	2 cut(s)	264, 1240
MvaI	CCWGG	1 cut(s)	276
MvnI	CGCG	4 cut(s)	137, 174, 547, 927
MwoI	GCNNNNNNNGC	4 cut(s)	171, 248, 784, 1129
NdeII	GATC	5 cut(s)	235, 892, 998, 1181, 1328
NlaIII	CATG	2 cut(s)	1261, 1324
NlaIV	GGNNCC	1 cut(s)	796
NsiI	ATGCAT	1 cut(s)	424
PaeR7I	CTCGAG	2 cut(s)	230, 969
PauI	GCGCGC	1 cut(s)	172
PcsI	WCGNNNNNNNCGW	1 cut(s)	98
PctI	GAATGC	2 cut(s)	264, 1240
PdmI	GAANNNNTTC	1 cut(s)	732
PfeI	GAWTC	3 cut(s)	179, 542, 1172
PkrI	GCNGC	5 cut(s)	867, 1099, 1240, 1256, 1273
PleI	GAGTC	2 cut(s)	698, 1134
PpsI	GAGTC	2 cut(s)	698, 1134
Psp6I	CCWGG	1 cut(s)	274
PspGI	CCWGG	1 cut(s)	274
PspN4I	GGNNCC	1 cut(s)	796
PspXI	VCTCGAGB	2 cut(s)	230, 969
PstI	CTGCAG	2 cut(s)	748, 1134
PstNI	CAGNNNCTG	2 cut(s)	1129, 1353
PsuI	RGATCY	1 cut(s)	998
PteI	GCGCGC	1 cut(s)	172
PvuII	CAGCTG	2 cut(s)	985, 1274
RsaI	GTAC	1 cut(s)	646
RsaNI	GTAC	1 cut(s)	645
RseI	CAYNNNNRTG	1 cut(s)	336
SacII	CCGCGG	1 cut(s)	138
SaqAI	TTAA	6 cut(s)	47, 291, 300, 513, 563, 876
SatI	GCNGC	5 cut(s)	866, 1098, 1239, 1255, 1272
Sau3AI	GATC	5 cut(s)	235, 892, 998, 1181, 1328
SchI	GAGTC	2 cut(s)	698, 1135
ScrFI	CCNGG	1 cut(s)	276
SduI	GDGCHC	4 cut(s)	133, 244, 725, 1400
SfaNI	GCATC	6 cut(s)	409, 431, 589, 629, 1109, 1236
SfcI	CTRYAG	3 cut(s)	648, 744, 1130
Sfr274I	CTCGAG	2 cut(s)	230, 969
Sfr303I	CCGCGG	1 cut(s)	138
SgrBI	CCGCGG	1 cut(s)	138
SlaI	CTCGAG	2 cut(s)	230, 969
SmiMI	CAYNNNNRTG	1 cut(s)	336
SmlI	CTYRAG	2 cut(s)	230, 969
SmoI	CTYRAG	2 cut(s)	230, 969
Sse9I	AATT	8 cut(s)	60, 281, 605, 731, 836, 881, 920, 1414
SsiI	CCGC	6 cut(s)	126, 135, 137, 201, 778, 925
SspI	AATATT	3 cut(s)	434, 450, 697
SspMI	CTAG	2 cut(s)	53, 1176
StyD4I	CCNGG	1 cut(s)	274
TaaI	ACNGT	1 cut(s)	1355
TaiI	ACGT	1 cut(s)	104
TaqI	TCGA	6 cut(s)	231, 255, 425, 656, 970, 1245
TaqII	GACCGA	1 cut(s)	1070
TasI	AATT	8 cut(s)	60, 281, 605, 731, 836, 881, 920, 1414
TfiI	GAWTC	3 cut(s)	179, 542, 1172
Tru1I	TTAA	6 cut(s)	47, 291, 300, 513, 563, 876
Tru9I	TTAA	6 cut(s)	47, 291, 300, 513, 563, 876
TscAI	CASTG	1 cut(s)	1393
TseI	GCWGC	5 cut(s)	865, 1097, 1238, 1254, 1271
TspDTI	ATGAA	3 cut(s)	930, 1197, 1309
TspRI	CASTG	1 cut(s)	1393
VneI	GTGCAC	1 cut(s)	1396
XapI	RAATTY	3 cut(s)	605, 731, 920
XhoI	CTCGAG	2 cut(s)	230, 969
XmnI	GAANNNNTTC	1 cut(s)	732
XspI	CTAG	2 cut(s)	53, 1176
Zsp2I	ATGCAT	1 cut(s)	424

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.