Serine/threonine phosphatases, family 2C, catalytic domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 2 cut(s) | 2333, 2498 |
| Acc36I | ACCTGC | 1 cut(s) | 1497 |
| AccI | GTMKAC | 1 cut(s) | 2824 |
| AccII | CGCG | 2 cut(s) | 58, 187 |
| AccIII | TCCGGA | 1 cut(s) | 808 |
| AciI | CCGC | 9 cut(s) | 105, 116, 235, 314, 870, 1439, 2380, 2974, 3037 |
| AclWI | GGATC | 7 cut(s) | 293, 756, 1306, 1687, 1747, 2710, 2930 |
| AcsI | RAATTY | 9 cut(s) | 582, 629, 755, 1690, 1960, 2072, 2463, 2479, 2906 |
| AcuI | CTGAAG | 4 cut(s) | 360, 423, 1782, 2622 |
| AcvI | CACGTG | 1 cut(s) | 2738 |
| AcyI | GRCGYC | 1 cut(s) | 8 |
| AfaI | GTAC | 8 cut(s) | 95, 1071, 1118, 1144, 1264, 1547, 1716, 2265 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 272, 277, 772, 1495, 1707 |
| AflII | CTTAAG | 1 cut(s) | 2942 |
| AflIII | ACRYGT | 1 cut(s) | 2735 |
| AhlI | ACTAGT | 2 cut(s) | 455, 2726 |
| AjiI | CACGTC | 1 cut(s) | 81 |
| AjnI | CCWGG | 4 cut(s) | 1084, 1494, 2888, 3004 |
| AjuI | GAANNNNNNNTTGG | 8 cut(s) | 353, 385, 510, 542, 1881, 1913, 2046, 2078 |
| AleI | CACNNNNGTG | 1 cut(s) | 1527 |
| Alw21I | GWGCWC | 1 cut(s) | 879 |
| Alw26I | GTCTC | 3 cut(s) | 720, 928, 1779 |
| Alw44I | GTGCAC | 1 cut(s) | 875 |
| AlwI | GGATC | 7 cut(s) | 293, 756, 1306, 1687, 1747, 2710, 2930 |
| Ama87I | CYCGRG | 2 cut(s) | 906, 2145 |
| Aor13HI | TCCGGA | 1 cut(s) | 808 |
| AoxI | GGCC | 3 cut(s) | 499, 1860, 1996 |
| ApaLI | GTGCAC | 1 cut(s) | 875 |
| ApeKI | GCWGC | 3 cut(s) | 1007, 1178, 1949 |
| ApoI | RAATTY | 9 cut(s) | 582, 629, 755, 1690, 1960, 2072, 2463, 2479, 2906 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1508, 1540, 2320, 2352 |
| Asp700I | GAANNNNTTC | 6 cut(s) | 365, 756, 1647, 1795, 1908, 2014 |
| AspLEI | GCGC | 7 cut(s) | 60, 110, 248, 2334, 2499, 2743, 2985 |
| AspS9I | GGNCC | 4 cut(s) | 232, 1860, 2561, 3001 |
| AsuC2I | CCSGG | 2 cut(s) | 273, 2835 |
| AsuHPI | GGTGA | 9 cut(s) | 11, 80, 902, 929, 1760, 2697, 2846, 2871, 3028 |
| AsuII | TTCGAA | 2 cut(s) | 359, 2139 |
| AsuNHI | GCTAGC | 1 cut(s) | 2788 |
| AvaI | CYCGRG | 2 cut(s) | 906, 2145 |
| AvaII | GGWCC | 3 cut(s) | 232, 2561, 3001 |
| BaeGI | GKGCMC | 1 cut(s) | 879 |
| BauI | CACGAG | 1 cut(s) | 1812 |
| BbrPI | CACGTG | 1 cut(s) | 2738 |
| BbsI | GAAGAC | 2 cut(s) | 2226, 2309 |
| Bbv12I | GWGCWC | 1 cut(s) | 879 |
| BbvI | GCAGC | 3 cut(s) | 994, 1190, 1936 |
| BceAI | ACGGC | 2 cut(s) | 32, 500 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1555, 1589 |
| BciT130I | CCWGG | 4 cut(s) | 1086, 1496, 2890, 3006 |
| BcnI | CCSGG | 2 cut(s) | 273, 2835 |
| BcoDI | GTCTC | 3 cut(s) | 720, 928, 1779 |
| BcuI | ACTAGT | 2 cut(s) | 455, 2726 |
| BfoI | RGCGCY | 1 cut(s) | 111 |
| BfrI | CTTAAG | 1 cut(s) | 2942 |
| BfuAI | ACCTGC | 1 cut(s) | 1497 |
| BglII | AGATCT | 2 cut(s) | 1096, 2268 |
| BisI | GCNGC | 6 cut(s) | 106, 1008, 1179, 1950, 2975, 3038 |
| BlsI | GCNGC | 6 cut(s) | 107, 1009, 1180, 1951, 2976, 3039 |
| Bme1390I | CCNGG | 6 cut(s) | 273, 1086, 1496, 2835, 2890, 3006 |
| Bme18I | GGWCC | 3 cut(s) | 232, 2561, 3001 |
| BmeT110I | CYCGRG | 2 cut(s) | 906, 2145 |
| BmgBI | CACGTC | 1 cut(s) | 81 |
| BmgT120I | GGNCC | 4 cut(s) | 232, 1860, 2561, 3001 |
| BmiI | GGNNCC | 5 cut(s) | 670, 1861, 2554, 3002, 3003 |
| BmrFI | CCNGG | 6 cut(s) | 273, 1086, 1496, 2835, 2890, 3006 |
| BmrI | ACTGGG | 1 cut(s) | 2454 |
| BmtI | GCTAGC | 1 cut(s) | 2792 |
| BmuI | ACTGGG | 1 cut(s) | 2454 |
| BpiI | GAAGAC | 2 cut(s) | 2226, 2309 |
| BpmI | CTGGAG | 1 cut(s) | 3027 |
| Bpu14I | TTCGAA | 2 cut(s) | 359, 2139 |
| BpuEI | CTTGAG | 1 cut(s) | 1789 |
| BpuMI | CCSGG | 2 cut(s) | 273, 2835 |
| BsaAI | YACGTR | 2 cut(s) | 1886, 2738 |
| BsaBI | GATNNNNATC | 4 cut(s) | 570, 804, 996, 1821 |
| BsaHI | GRCGYC | 1 cut(s) | 8 |
| BsaJI | CCNNGG | 6 cut(s) | 271, 1085, 1494, 1573, 2833, 3004 |
| BsaWI | WCCGGW | 1 cut(s) | 808 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1433, 1463, 2659, 2689, 3000, 3030 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 272, 277, 772, 1495, 1707 |
| Bse1I | ACTGG | 4 cut(s) | 1349, 1623, 2449, 2563 |
| Bse3DI | GCAATG | 1 cut(s) | 2526 |
| Bse8I | GATNNNNATC | 4 cut(s) | 570, 804, 996, 1821 |
| BseAI | TCCGGA | 1 cut(s) | 808 |
| BseBI | CCWGG | 4 cut(s) | 1086, 1496, 2890, 3006 |
| BseDI | CCNNGG | 6 cut(s) | 271, 1085, 1494, 1573, 2833, 3004 |
| BseJI | GATNNNNATC | 4 cut(s) | 570, 804, 996, 1821 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 272, 277, 772, 1495, 1707 |
| BseMI | GCAATG | 1 cut(s) | 2526 |
| BseMII | CTCAG | 2 cut(s) | 762, 1327 |
| BseNI | ACTGG | 4 cut(s) | 1349, 1623, 2449, 2563 |
| BseRI | GAGGAG | 2 cut(s) | 2586, 3051 |
| BseSI | GKGCMC | 1 cut(s) | 879 |
| BseXI | GCAGC | 3 cut(s) | 994, 1190, 1936 |
| BsgI | GTGCAG | 2 cut(s) | 789, 936 |
| Bsh1236I | CGCG | 2 cut(s) | 58, 187 |
| BshFI | GGCC | 3 cut(s) | 501, 1862, 1998 |
| BsiHKAI | GWGCWC | 1 cut(s) | 879 |
| BsiHKCI | CYCGRG | 2 cut(s) | 906, 2145 |
| BsiSI | CCGG | 5 cut(s) | 113, 217, 273, 809, 2835 |
| BslFI | GGGAC | 1 cut(s) | 3014 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 272, 277, 772, 1495, 1707 |
| BsmAI | GTCTC | 3 cut(s) | 720, 928, 1779 |
| BsmFI | GGGAC | 1 cut(s) | 3014 |
| BsmI | GAATGC | 2 cut(s) | 261, 2233 |
| BsnI | GGCC | 3 cut(s) | 501, 1862, 1998 |
| BsoBI | CYCGRG | 2 cut(s) | 906, 2145 |
| Bsp119I | TTCGAA | 2 cut(s) | 359, 2139 |
| Bsp1286I | GDGCHC | 1 cut(s) | 879 |
| Bsp13I | TCCGGA | 1 cut(s) | 808 |
| Bsp1407I | TGTACA | 2 cut(s) | 93, 2263 |
| BspACI | CCGC | 9 cut(s) | 105, 116, 235, 314, 870, 1439, 2380, 2974, 3037 |
| BspANI | GGCC | 3 cut(s) | 501, 1862, 1998 |
| BspCNI | CTCAG | 2 cut(s) | 761, 1326 |
| BspEI | TCCGGA | 1 cut(s) | 808 |
| BspFNI | CGCG | 2 cut(s) | 58, 187 |
| BspHI | TCATGA | 2 cut(s) | 613, 2374 |
| BspLI | GGNNCC | 5 cut(s) | 670, 1861, 2554, 3002, 3003 |
| BspMI | ACCTGC | 1 cut(s) | 1497 |
| BspOI | GCTAGC | 1 cut(s) | 2792 |
| BspPI | GGATC | 7 cut(s) | 293, 756, 1306, 1687, 1747, 2710, 2930 |
| BspQI | GCTCTTC | 2 cut(s) | 1506, 2427 |
| BspT104I | TTCGAA | 2 cut(s) | 359, 2139 |
| BspTI | CTTAAG | 1 cut(s) | 2942 |
| BsrDI | GCAATG | 1 cut(s) | 2526 |
| BsrGI | TGTACA | 2 cut(s) | 93, 2263 |
| BsrI | ACTGG | 4 cut(s) | 1349, 1623, 2449, 2563 |
| BssECI | CCNNGG | 6 cut(s) | 271, 1085, 1494, 1573, 2833, 3004 |
| BssNAI | GTATAC | 1 cut(s) | 2825 |
| BssNI | GRCGYC | 1 cut(s) | 8 |
| BssSI | CACGAG | 1 cut(s) | 1812 |
| Bst1107I | GTATAC | 1 cut(s) | 2825 |
| Bst2BI | CACGAG | 1 cut(s) | 1812 |
| Bst2UI | CCWGG | 4 cut(s) | 1086, 1496, 2890, 3006 |
| Bst4CI | ACNGT | 9 cut(s) | 68, 91, 301, 559, 682, 1220, 1267, 1867, 2212 |
| Bst6I | CTCTTC | 3 cut(s) | 1506, 1718, 2427 |
| BstACI | GRCGYC | 1 cut(s) | 8 |
| BstAFI | CTTAAG | 1 cut(s) | 2942 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 243, 692 |
| BstAUI | TGTACA | 2 cut(s) | 93, 2263 |
| BstBAI | YACGTR | 2 cut(s) | 1886, 2738 |
| BstBI | TTCGAA | 2 cut(s) | 359, 2139 |
| BstC8I | GCNNGC | 4 cut(s) | 1568, 1761, 2790, 2987 |
| BstDEI | CTNAG | 9 cut(s) | 423, 636, 709, 748, 1313, 1538, 2686, 2698, 2801 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1493 |
| BstFNI | CGCG | 2 cut(s) | 58, 187 |
| BstH2I | RGCGCY | 1 cut(s) | 111 |
| BstHHI | GCGC | 7 cut(s) | 60, 110, 248, 2334, 2499, 2743, 2985 |
| BstMAI | GTCTC | 3 cut(s) | 720, 928, 1779 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 243, 683, 692, 1401, 1614, 2882, 2980 |
| BstNI | CCWGG | 4 cut(s) | 1086, 1496, 2890, 3006 |
| BstNSI | RCATGY | 4 cut(s) | 1439, 1511, 1570, 2264 |
| BstSCI | CCNGG | 6 cut(s) | 271, 1084, 1494, 2833, 2888, 3004 |
| BstSLI | GKGCMC | 1 cut(s) | 879 |
| BstUI | CGCG | 2 cut(s) | 58, 187 |
| BstV1I | GCAGC | 3 cut(s) | 994, 1190, 1936 |
| BstV2I | GAAGAC | 2 cut(s) | 2226, 2309 |
| BstX2I | RGATCY | 4 cut(s) | 1096, 1739, 2268, 2935 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 1526, 2286, 2889, 2964 |
| BstYI | RGATCY | 4 cut(s) | 1096, 1739, 2268, 2935 |
| BstZ17I | GTATAC | 1 cut(s) | 2825 |
| BsuRI | GGCC | 3 cut(s) | 501, 1862, 1998 |
| BtgZI | GCGATG | 1 cut(s) | 215 |
| BtrI | CACGTC | 1 cut(s) | 81 |
| BtsIMutI | CAGTG | 3 cut(s) | 1863, 2442, 2556 |
| BveI | ACCTGC | 1 cut(s) | 1497 |
| Cac8I | GCNNGC | 4 cut(s) | 1568, 1761, 2790, 2987 |
| CciI | TCATGA | 2 cut(s) | 613, 2374 |
| CfoI | GCGC | 7 cut(s) | 60, 110, 248, 2334, 2499, 2743, 2985 |
| Cfr13I | GGNCC | 4 cut(s) | 232, 1860, 2561, 3001 |
| CseI | GACGC | 2 cut(s) | 999, 2264 |
| Csp6I | GTAC | 8 cut(s) | 94, 1070, 1117, 1143, 1263, 1546, 1715, 2264 |
| CviQI | GTAC | 8 cut(s) | 94, 1070, 1117, 1143, 1263, 1546, 1715, 2264 |
| DdeI | CTNAG | 9 cut(s) | 423, 636, 709, 748, 1313, 1538, 2686, 2698, 2801 |
| DraI | TTTAAA | 1 cut(s) | 931 |
| Eam1104I | CTCTTC | 3 cut(s) | 1506, 1718, 2427 |
| EarI | CTCTTC | 3 cut(s) | 1506, 1718, 2427 |
| Eco32I | GATATC | 1 cut(s) | 2401 |
| Eco47I | GGWCC | 3 cut(s) | 232, 2561, 3001 |
| Eco57I | CTGAAG | 4 cut(s) | 360, 423, 1782, 2622 |
| Eco72I | CACGTG | 1 cut(s) | 2738 |
| Eco88I | CYCGRG | 2 cut(s) | 906, 2145 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1493 |
| EcoO109I | RGGNCCY | 1 cut(s) | 3001 |
| EcoRI | GAATTC | 2 cut(s) | 1690, 2463 |
| EcoRII | CCWGG | 4 cut(s) | 1084, 1494, 2888, 3004 |
| EcoRV | GATATC | 1 cut(s) | 2401 |
| EcoT22I | ATGCAT | 3 cut(s) | 412, 1610, 1921 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 353, 385, 1896, 1928, 2595, 2627 |
| FaqI | GGGAC | 1 cut(s) | 3014 |
| FblI | GTMKAC | 1 cut(s) | 2824 |
| Fnu4HI | GCNGC | 6 cut(s) | 106, 1008, 1179, 1950, 2975, 3038 |
| Fsp4HI | GCNGC | 6 cut(s) | 106, 1008, 1179, 1950, 2975, 3038 |
| FspAI | RTGCGCAY | 1 cut(s) | 2498 |
| FspI | TGCGCA | 2 cut(s) | 2333, 2498 |
| GlaI | GCGC | 7 cut(s) | 59, 109, 247, 2333, 2498, 2742, 2984 |
| GluI | GCNGC | 6 cut(s) | 106, 1008, 1179, 1950, 2975, 3038 |
| GsuI | CTGGAG | 1 cut(s) | 3027 |
| HaeII | RGCGCY | 1 cut(s) | 111 |
| HaeIII | GGCC | 3 cut(s) | 501, 1862, 1998 |
| HapII | CCGG | 5 cut(s) | 113, 217, 273, 809, 2835 |
| HgaI | GACGC | 2 cut(s) | 999, 2264 |
| HhaI | GCGC | 7 cut(s) | 60, 110, 248, 2334, 2499, 2743, 2985 |
| Hin1I | GRCGYC | 1 cut(s) | 8 |
| Hin6I | GCGC | 7 cut(s) | 58, 108, 246, 2332, 2497, 2741, 2983 |
| HinP1I | GCGC | 7 cut(s) | 58, 108, 246, 2332, 2497, 2741, 2983 |
| HincII | GTYRAC | 2 cut(s) | 12, 1287 |
| HindII | GTYRAC | 2 cut(s) | 12, 1287 |
| HindIII | AAGCTT | 5 cut(s) | 362, 435, 2056, 2198, 2798 |
| HpaII | CCGG | 5 cut(s) | 113, 217, 273, 809, 2835 |
| HphI | GGTGA | 9 cut(s) | 11, 80, 902, 929, 1760, 2697, 2846, 2871, 3028 |
| Hpy99I | CGWCG | 2 cut(s) | 82, 486 |
| HpyCH4III | ACNGT | 9 cut(s) | 68, 91, 301, 559, 682, 1220, 1267, 1867, 2212 |
| HpyCH4IV | ACGT | 4 cut(s) | 80, 1173, 1885, 2737 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 243, 683, 692, 1401, 1614, 2882, 2980 |
| HpyF3I | CTNAG | 9 cut(s) | 423, 636, 709, 748, 1313, 1538, 2686, 2698, 2801 |
| HpySE526I | ACGT | 4 cut(s) | 80, 1173, 1885, 2737 |
| Hsp92I | GRCGYC | 1 cut(s) | 8 |
| HspAI | GCGC | 7 cut(s) | 58, 108, 246, 2332, 2497, 2741, 2983 |
| KflI | GGGWCCC | 1 cut(s) | 3001 |
| Kpn2I | TCCGGA | 1 cut(s) | 808 |
| LguI | GCTCTTC | 2 cut(s) | 1506, 2427 |
| LmnI | GCTCC | 6 cut(s) | 115, 335, 2323, 2785, 2815, 2873 |
| Lsp1109I | GCAGC | 3 cut(s) | 994, 1190, 1936 |
| MaeII | ACGT | 4 cut(s) | 80, 1173, 1885, 2737 |
| MaeIII | GTNAC | 5 cut(s) | 295, 943, 1316, 1778, 2729 |
| MfeI | CAATTG | 1 cut(s) | 1331 |
| MflI | RGATCY | 4 cut(s) | 1096, 1739, 2268, 2935 |
| MhlI | GDGCHC | 1 cut(s) | 879 |
| MlyI | GAGTC | 3 cut(s) | 722, 1796, 2141 |
| MmeI | TCCRAC | 4 cut(s) | 283, 1751, 2024, 2851 |
| Mph1103I | ATGCAT | 3 cut(s) | 412, 1610, 1921 |
| MroI | TCCGGA | 1 cut(s) | 808 |
| MroXI | GAANNNNTTC | 6 cut(s) | 365, 756, 1647, 1795, 1908, 2014 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1395, 1527, 2284, 2492, 2547 |
| MspA1I | CMGCKG | 2 cut(s) | 1181, 1949 |
| MspCI | CTTAAG | 1 cut(s) | 2942 |
| MspI | CCGG | 5 cut(s) | 113, 217, 273, 809, 2835 |
| MspR9I | CCNGG | 6 cut(s) | 273, 1086, 1496, 2835, 2890, 3006 |
| MunI | CAATTG | 1 cut(s) | 1331 |
| Mva1269I | GAATGC | 2 cut(s) | 261, 2233 |
| MvaI | CCWGG | 4 cut(s) | 1086, 1496, 2890, 3006 |
| MvnI | CGCG | 2 cut(s) | 58, 187 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 243, 683, 692, 1401, 1614, 2882, 2980 |
| NciI | CCSGG | 2 cut(s) | 273, 2835 |
| NheI | GCTAGC | 1 cut(s) | 2788 |
| NlaIV | GGNNCC | 5 cut(s) | 670, 1861, 2554, 3002, 3003 |
| NmuCI | GTSAC | 1 cut(s) | 1778 |
| NsbI | TGCGCA | 2 cut(s) | 2333, 2498 |
| NsiI | ATGCAT | 3 cut(s) | 412, 1610, 1921 |
| NspI | RCATGY | 4 cut(s) | 1439, 1511, 1570, 2264 |
| NspV | TTCGAA | 2 cut(s) | 359, 2139 |
| OliI | CACNNNNGTG | 1 cut(s) | 1527 |
| PaeI | GCATGC | 1 cut(s) | 1570 |
| PagI | TCATGA | 2 cut(s) | 613, 2374 |
| PciSI | GCTCTTC | 2 cut(s) | 1506, 2427 |
| PctI | GAATGC | 2 cut(s) | 261, 2233 |
| PdmI | GAANNNNTTC | 6 cut(s) | 365, 756, 1647, 1795, 1908, 2014 |
| PfeI | GAWTC | 9 cut(s) | 71, 170, 566, 662, 1643, 1696, 2015, 2141, 3071 |
| PfoI | TCCNGGA | 1 cut(s) | 2888 |
| PkrI | GCNGC | 6 cut(s) | 107, 1009, 1180, 1951, 2976, 3039 |
| PleI | GAGTC | 3 cut(s) | 722, 1796, 2140 |
| PmaCI | CACGTG | 1 cut(s) | 2738 |
| PmlI | CACGTG | 1 cut(s) | 2738 |
| PpsI | GAGTC | 3 cut(s) | 722, 1796, 2140 |
| Ppu21I | YACGTR | 2 cut(s) | 1886, 2738 |
| PpuMI | RGGWCCY | 1 cut(s) | 3001 |
| Psp5II | RGGWCCY | 1 cut(s) | 3001 |
| Psp6I | CCWGG | 4 cut(s) | 1084, 1494, 2888, 3004 |
| PspCI | CACGTG | 1 cut(s) | 2738 |
| PspGI | CCWGG | 4 cut(s) | 1084, 1494, 2888, 3004 |
| PspN4I | GGNNCC | 5 cut(s) | 670, 1861, 2554, 3002, 3003 |
| PspPI | GGNCC | 4 cut(s) | 232, 1860, 2561, 3001 |
| PspPPI | RGGWCCY | 1 cut(s) | 3001 |
| PsuI | RGATCY | 4 cut(s) | 1096, 1739, 2268, 2935 |
| PvuII | CAGCTG | 2 cut(s) | 1181, 1949 |
| RsaI | GTAC | 8 cut(s) | 95, 1071, 1118, 1144, 1264, 1547, 1716, 2265 |
| RsaNI | GTAC | 8 cut(s) | 94, 1070, 1117, 1143, 1263, 1546, 1715, 2264 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1395, 1527, 2284, 2492, 2547 |
| SapI | GCTCTTC | 2 cut(s) | 1506, 2427 |
| SatI | GCNGC | 6 cut(s) | 106, 1008, 1179, 1950, 2975, 3038 |
| Sau96I | GGNCC | 4 cut(s) | 232, 1860, 2561, 3001 |
| SchI | GAGTC | 3 cut(s) | 722, 1796, 2141 |
| ScrFI | CCNGG | 6 cut(s) | 273, 1086, 1496, 2835, 2890, 3006 |
| SduI | GDGCHC | 1 cut(s) | 879 |
| SfuI | TTCGAA | 2 cut(s) | 359, 2139 |
| SinI | GGWCC | 3 cut(s) | 232, 2561, 3001 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1395, 1527, 2284, 2492, 2547 |
| SmlI | CTYRAG | 2 cut(s) | 1804, 2942 |
| SmoI | CTYRAG | 2 cut(s) | 1804, 2942 |
| SpeI | ACTAGT | 2 cut(s) | 455, 2726 |
| SphI | GCATGC | 1 cut(s) | 1570 |
| SsiI | CCGC | 9 cut(s) | 105, 116, 235, 314, 870, 1439, 2380, 2974, 3037 |
| SspI | AATATT | 2 cut(s) | 721, 2386 |
| StyD4I | CCNGG | 6 cut(s) | 271, 1084, 1494, 2833, 2888, 3004 |
| TaaI | ACNGT | 9 cut(s) | 68, 91, 301, 559, 682, 1220, 1267, 1867, 2212 |
| TaiI | ACGT | 4 cut(s) | 83, 1176, 1888, 2740 |
| TaqII | GACCGA | 2 cut(s) | 1797, 2880 |
| TatI | WGTACW | 4 cut(s) | 93, 1116, 1714, 2263 |
| TauI | GCSGC | 3 cut(s) | 108, 2977, 3040 |
| TfiI | GAWTC | 9 cut(s) | 71, 170, 566, 662, 1643, 1696, 2015, 2141, 3071 |
| TscAI | CASTG | 3 cut(s) | 1870, 2449, 2563 |
| TseFI | GTSAC | 1 cut(s) | 1778 |
| TseI | GCWGC | 3 cut(s) | 1007, 1178, 1949 |
| Tsp45I | GTSAC | 1 cut(s) | 1778 |
| TspGWI | ACGGA | 2 cut(s) | 980, 1855 |
| TspRI | CASTG | 3 cut(s) | 1870, 2449, 2563 |
| Vha464I | CTTAAG | 1 cut(s) | 2942 |
| VneI | GTGCAC | 1 cut(s) | 875 |
| VpaK11BI | GGWCC | 3 cut(s) | 232, 2561, 3001 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1493 |
| XapI | RAATTY | 9 cut(s) | 582, 629, 755, 1690, 1960, 2072, 2463, 2479, 2906 |
| XceI | RCATGY | 4 cut(s) | 1439, 1511, 1570, 2264 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1526, 2286 |
| XmiI | GTMKAC | 1 cut(s) | 2824 |
| XmnI | GAANNNNTTC | 6 cut(s) | 365, 756, 1647, 1795, 1908, 2014 |
| Zsp2I | ATGCAT | 3 cut(s) | 412, 1610, 1921 |