Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000935

Physical Location & Seq

Genomic Coordinates Forward (+)

26372702 .. 26374915

Gene Structure 2214 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1204000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1362 bp

ATGGAGTGCTCCGGAGTCCTCCACCTAATCGTACTGGGATTACTATTCGCTGGCCTTCCGACGCTCTCCTACGGAAGAGAATCGTCGTCGACGTGCTTGACGCTGTACGAACATGGTGGCGCTCCGGCGGTTTTTGAATCCCCAGAATGCCGTCAATTGAAGCATTCCGACGACTCCCAACGCACGTCGCTTTGTCAAATGGCCATGCTTCAAGGTCGAAGAAAGTACTTGGAGGATCGCACTCTCTGCGCTCTCGACGTTCGCATTCTCTTTCCTGGTAGAGTAGGGATTATGGAGGAGGTTAGAGTTGGAGTTGCTGCTGTTTTTGATGGTCATAATGGTGCTGAAGCTAGTGATATGGCTTCCAAGCTTTTCTTGGAGTACTTCGTTTTGCATACCTACTTTCTCCTCGATGCATCGCTTTCCGCTGAGTCATCTGAAACACCAACATTGCAAAATTGGCATGGCTTACTCGATCGGGGCCAATTGGATATTGCAAGGTTTAAGCATTCGTTGCAAGCAAACCTTGACCATTCTTTTCGTTTCGACATCTTGAAAGAAGCACTGTTGAGGGCAGTTCACGATATTGATGCAAAGTTTTCTAAGGCTGCATTTATGGAGAATCTAAATTCCGGTTCTACTGCCACAATTATACTACTTTTGGATGGTCAAATCTTAGTTGCCAATATTGGAGACTCAAAGGCCTTTTTGTGCTCGGAAAAATTTCAAACTCCTGCAGAGGCTAAAGCTGCGTATTTAAGATTGTACATGGAGGAAAGGCGTAATGGTGTTGTCTCACATCGATGGCGCAATAAAAACTTTGAATTAGCTCCCACGGATGAGTTGACGCATTTATCTGTTAAGGAATTGACAAGAGATCATCATCCGGACAGAGACGATGAAAAACGTAGGGTGGAAAGTGCTGGTGGAAATGTTTTTGAATGGGCTGGTGTGCCTCGAGTAAATGGTCAGCTGGCTGTTACCCGATCTATTGGCGACATCTCCTTTAAAAGTTTTGGCGTTGTATCAGAACCGGAGTTGACAGATTGGCAAACCCTGACTGTAAATGATACATATCTCGTGGCTACATCTGACGGAGTTTTTGAGAAGCAGAGCTTGCAGGATGTTTGCGATCTGTTGTGGGAAGCACGAAGTCATCATCCTACGAAATTGGAGCTCTCTTCGGCAGGTTCATACTCATTAGCTGACCGTATTGTAAGTACTGCATTTGAAAGGGGCAGTATCGATAATATGGCAGCTGTTGTAGTTCCATTGGCATCTTCTGGTTTATCTCAACCGGAGCCATTGCTACGTGTTTTCTCATTTGCAGCTAACAACTCCTCTTTAGTTCGTATAGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C Pkinase

Protein Analysis

454

Amino Acids

50.14

Weight (kDa)

5.55

Isoelectric Point (pI)

42.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	177 - 240	1.1e-07	Protein phosphatase 2C
PP2C	PF00481	288 - 418	3.6e-25	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000935)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63320 AT3G63320 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340
fragaria_vesca	FvH4_2g23010 FvH4_2g23010 FvH4_2g23010 FvH4_2g23010 FvH4_2g23010
malus_domestica	MD00G1147300.v1.1 MD00G1147600.v1.1 MD00G1147700.v1.1 MD00G1147800.v1.1 MD03G1258500.v1.1 MD08G1204000.v1.1
prunus_persica	Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1
pyrus_communis	pycom03g20470 pycom08g17600 pycom08g17610 pycom08g17640 pycom08g17650 pycom08g17660
rosa_chinensis	RchiOBHm_Chr3g0479641 RchiOBHm_Chr6g0290051
rosa_laevigata	RLG00000012250 RLG00000023551 RLG00000023553 RLG00000023556 RLG00000029889
rosa_multiflora	Rmu_co8325477.1_g000001 Rmu_sc0001775.1_g000013 Rmu_sc0002902.1_g000039
rosa_roxburghii	Rroxscaffold_6G00402100 Rroxscaffold_6G00402120 Rroxscaffold_7G00177390
rosa_rugosa	Rorug03G0176900 Rorug03G0177000 Rorug06G0209000 Rorug06G0209100
rosa_samantha	Rh3AG227700 Rh3BG260800 Rh3BG261000 Rh3CG258100 Rh3DG254200 Rh6AG320000 Rh6BG327200 Rh6CG332900 Rh6DG318800
rosa_wichuraiana	Rw3G020380 Rw6G027610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1178
AccI	GTMKAC	1 cut(s)	89
AccIII	TCCGGA	2 cut(s)	11, 886
AciI	CCGC	2 cut(s)	128, 426
AclWI	GGATC	1 cut(s)	243
AcoI	YGGCCR	1 cut(s)	201
AcsI	RAATTY	2 cut(s)	628, 722
AcuI	CTGAAG	1 cut(s)	366
AfaI	GTAC	6 cut(s)	33, 107, 227, 383, 767, 1222
AflIII	ACRYGT	1 cut(s)	1312
AgsI	TTSAA	8 cut(s)	137, 160, 212, 556, 728, 824, 941, 1232
AjiI	CACGTC	2 cut(s)	93, 186
AjnI	CCWGG	1 cut(s)	274
Alw21I	GWGCWC	3 cut(s)	11, 716, 1179
Alw26I	GTCTC	3 cut(s)	687, 799, 888
AlwI	GGATC	1 cut(s)	243
Ama87I	CYCGRG	1 cut(s)	957
Aor13HI	TCCGGA	2 cut(s)	11, 886
AoxI	GGCC	4 cut(s)	52, 201, 481, 702
ApeKI	GCWGC	5 cut(s)	317, 608, 749, 1256, 1328
ApoI	RAATTY	2 cut(s)	628, 722
ArsI	GACNNNNNNTTYG	2 cut(s)	1085, 1117
Asp700I	GAANNNNTTC	1 cut(s)	723
AspLEI	GCGC	3 cut(s)	122, 251, 810
AspS9I	GGNCC	1 cut(s)	481
AvaI	CYCGRG	1 cut(s)	957
BalI	TGGCCA	1 cut(s)	203
BanII	GRGCYC	1 cut(s)	1179
BauI	CACGAG	1 cut(s)	1079
Bbv12I	GWGCWC	3 cut(s)	11, 716, 1179
BbvI	GCAGC	5 cut(s)	304, 595, 736, 1268, 1340
BccI	CCATC	3 cut(s)	323, 659, 798
BceAI	ACGGC	1 cut(s)	135
BcgI	CGANNNNNNTGC	2 cut(s)	572, 606
BciT130I	CCWGG	1 cut(s)	276
BcoDI	GTCTC	3 cut(s)	687, 799, 888
BfaI	CTAG	1 cut(s)	351
BfmI	CTRYAG	1 cut(s)	735
BfoI	RGCGCY	1 cut(s)	123
BfuAI	ACCTGC	1 cut(s)	1178
BisI	GCNGC	5 cut(s)	318, 609, 750, 1257, 1329
BlsI	GCNGC	5 cut(s)	319, 610, 751, 1258, 1330
BmcAI	AGTACT	3 cut(s)	227, 383, 1222
Bme1390I	CCNGG	1 cut(s)	276
BmeT110I	CYCGRG	1 cut(s)	957
BmgBI	CACGTC	2 cut(s)	93, 186
BmgT120I	GGNCC	1 cut(s)	481
BmiI	GGNNCC	2 cut(s)	482, 1302
BmrFI	CCNGG	1 cut(s)	276
BmrI	ACTGGG	1 cut(s)	44
BmsI	GCATC	4 cut(s)	403, 425, 580, 1286
BmuI	ACTGGG	1 cut(s)	44
Bsa29I	ATCGAT	2 cut(s)	802, 1245
BsaAI	YACGTR	1 cut(s)	1313
BsaBI	GATNNNNATC	2 cut(s)	882, 1074
BsaJI	CCNNGG	1 cut(s)	834
BsaWI	WCCGGW	5 cut(s)	11, 632, 886, 1033, 1297
Bse1I	ACTGG	1 cut(s)	39
Bse3DI	GCAATG	2 cut(s)	449, 1304
Bse8I	GATNNNNATC	2 cut(s)	882, 1074
BseAI	TCCGGA	2 cut(s)	11, 886
BseBI	CCWGG	1 cut(s)	276
BseCI	ATCGAT	2 cut(s)	802, 1245
BseDI	CCNNGG	1 cut(s)	834
BseGI	GGATG	5 cut(s)	670, 844, 883, 1129, 1159
BseJI	GATNNNNATC	2 cut(s)	882, 1074
BseMI	GCAATG	2 cut(s)	449, 1304
BseMII	CTCAG	1 cut(s)	420
BseNI	ACTGG	1 cut(s)	39
BseRI	GAGGAG	3 cut(s)	311, 398, 1330
BseXI	GCAGC	5 cut(s)	304, 595, 736, 1268, 1340
Bsh1285I	CGRYCG	1 cut(s)	478
BshFI	GGCC	4 cut(s)	54, 203, 483, 704
BshVI	ATCGAT	2 cut(s)	802, 1245
BsiEI	CGRYCG	1 cut(s)	478
BsiHKAI	GWGCWC	3 cut(s)	11, 716, 1179
BsiHKCI	CYCGRG	1 cut(s)	957
BsiSI	CCGG	6 cut(s)	12, 125, 633, 887, 1034, 1298
BsmAI	GTCTC	3 cut(s)	687, 799, 888
BsmBI	CGTCTC	1 cut(s)	888
BsmI	GAATGC	4 cut(s)	152, 163, 264, 508
BsnI	GGCC	4 cut(s)	54, 203, 483, 704
BsoBI	CYCGRG	1 cut(s)	957
Bsp1286I	GDGCHC	3 cut(s)	11, 716, 1179
Bsp13I	TCCGGA	2 cut(s)	11, 886
Bsp1407I	TGTACA	1 cut(s)	765
Bsp143I	GATC	5 cut(s)	235, 475, 877, 986, 1132
BspACI	CCGC	2 cut(s)	128, 426
BspANI	GGCC	4 cut(s)	54, 203, 483, 704
BspCNI	CTCAG	1 cut(s)	421
BspDI	ATCGAT	2 cut(s)	802, 1245
BspEI	TCCGGA	2 cut(s)	11, 886
BspLI	GGNNCC	2 cut(s)	482, 1302
BspMAI	CTGCAG	1 cut(s)	739
BspMI	ACCTGC	1 cut(s)	1178
BspPI	GGATC	1 cut(s)	243
BsrDI	GCAATG	2 cut(s)	449, 1304
BsrGI	TGTACA	1 cut(s)	765
BsrI	ACTGG	1 cut(s)	39
BssECI	CCNNGG	1 cut(s)	834
BssMI	GATC	5 cut(s)	235, 475, 877, 986, 1132
BssSI	CACGAG	1 cut(s)	1079
Bst2BI	CACGAG	1 cut(s)	1079
Bst2UI	CCWGG	1 cut(s)	276
Bst4CI	ACNGT	3 cut(s)	567, 1063, 1211
Bst6I	CTCTTC	2 cut(s)	70, 1186
BstAPI	GCANNNNNTGC	3 cut(s)	246, 514, 1117
BstAUI	TGTACA	1 cut(s)	765
BstBAI	YACGTR	1 cut(s)	1313
BstC8I	GCNNGC	4 cut(s)	52, 519, 975, 1118
BstDEI	CTNAG	3 cut(s)	429, 603, 676
BstDSI	CCRYGG	1 cut(s)	834
BstF5I	GGATG	5 cut(s)	670, 844, 883, 1129, 1159
BstH2I	RGCGCY	1 cut(s)	123
BstHHI	GCGC	3 cut(s)	122, 251, 810
BstKTI	GATC	5 cut(s)	238, 478, 880, 989, 1135
BstMAI	GTCTC	3 cut(s)	687, 799, 888
BstMBI	GATC	5 cut(s)	235, 475, 877, 986, 1132
BstMCI	CGRYCG	1 cut(s)	478
BstMWI	GCNNNNNNNGC	5 cut(s)	246, 460, 514, 749, 1117
BstNI	CCWGG	1 cut(s)	276
BstSCI	CCNGG	1 cut(s)	274
BstSFI	CTRYAG	1 cut(s)	735
BstV1I	GCAGC	5 cut(s)	304, 595, 736, 1268, 1340
Bsu15I	ATCGAT	2 cut(s)	802, 1245
BsuRI	GGCC	4 cut(s)	54, 203, 483, 704
BsuTUI	ATCGAT	2 cut(s)	802, 1245
BtgI	CCRYGG	1 cut(s)	834
BtgZI	GCGATG	1 cut(s)	402
BtrI	CACGTC	2 cut(s)	93, 186
BtsCI	GGATG	5 cut(s)	670, 844, 883, 1129, 1159
BtsIMutI	CAGTG	1 cut(s)	563
BveI	ACCTGC	1 cut(s)	1178
Cac8I	GCNNGC	4 cut(s)	52, 519, 975, 1118
CfoI	GCGC	3 cut(s)	122, 251, 810
Cfr13I	GGNCC	1 cut(s)	481
ClaI	ATCGAT	2 cut(s)	802, 1245
CseI	GACGC	3 cut(s)	70, 109, 856
Csp6I	GTAC	6 cut(s)	32, 106, 226, 382, 766, 1221
CviAII	CATG	4 cut(s)	113, 205, 464, 769
CviQI	GTAC	6 cut(s)	32, 106, 226, 382, 766, 1221
DdeI	CTNAG	3 cut(s)	429, 603, 676
DpnI	GATC	5 cut(s)	237, 477, 879, 988, 1134
DpnII	GATC	5 cut(s)	235, 475, 877, 986, 1132
DraI	TTTAAA	1 cut(s)	1009
EaeI	YGGCCR	1 cut(s)	201
Eam1104I	CTCTTC	2 cut(s)	70, 1186
EarI	CTCTTC	2 cut(s)	70, 1186
Ecl136II	GAGCTC	1 cut(s)	1177
Eco147I	AGGCCT	1 cut(s)	704
Eco24I	GRGCYC	1 cut(s)	1179
Eco53kI	GAGCTC	1 cut(s)	1177
Eco57I	CTGAAG	1 cut(s)	366
Eco88I	CYCGRG	1 cut(s)	957
EcoICRI	GAGCTC	1 cut(s)	1177
EcoRII	CCWGG	1 cut(s)	274
EcoT22I	ATGCAT	1 cut(s)	418
EcoT38I	GRGCYC	1 cut(s)	1179
Esp3I	CGTCTC	1 cut(s)	888
FaeI	CATG	4 cut(s)	116, 208, 467, 772
FalI	AAGNNNNNCTT	6 cut(s)	359, 391, 510, 542, 1100, 1132
FatI	CATG	4 cut(s)	112, 204, 463, 768
FblI	GTMKAC	1 cut(s)	89
Fnu4HI	GCNGC	5 cut(s)	318, 609, 750, 1257, 1329
FokI	GGATG	5 cut(s)	677, 851, 870, 1136, 1146
FriOI	GRGCYC	1 cut(s)	1179
Fsp4HI	GCNGC	5 cut(s)	318, 609, 750, 1257, 1329
FspBI	CTAG	1 cut(s)	351
GlaI	GCGC	3 cut(s)	121, 250, 809
GluI	GCNGC	5 cut(s)	318, 609, 750, 1257, 1329
HaeII	RGCGCY	1 cut(s)	123
HaeIII	GGCC	4 cut(s)	54, 203, 483, 704
HapII	CCGG	6 cut(s)	12, 125, 633, 887, 1034, 1298
HgaI	GACGC	3 cut(s)	70, 109, 856
HhaI	GCGC	3 cut(s)	122, 251, 810
Hin1II	CATG	4 cut(s)	116, 208, 467, 772
Hin6I	GCGC	3 cut(s)	120, 249, 808
HinP1I	GCGC	3 cut(s)	120, 249, 808
HincII	GTYRAC	3 cut(s)	90, 846, 1041
HindII	GTYRAC	3 cut(s)	90, 846, 1041
HindIII	AAGCTT	1 cut(s)	368
HinfI	GANTC	7 cut(s)	15, 80, 137, 173, 431, 622, 695
HpaII	CCGG	6 cut(s)	12, 125, 633, 887, 1034, 1298
Hpy166II	GTNNAC	4 cut(s)	90, 580, 846, 1041
Hpy188I	TCNGA	6 cut(s)	60, 169, 439, 718, 1030, 1093
Hpy188III	TCNNGA	5 cut(s)	12, 254, 553, 581, 887
Hpy8I	GTNNAC	4 cut(s)	90, 580, 846, 1041
Hpy99I	CGWCG	7 cut(s)	64, 88, 91, 94, 173, 190, 260
HpyAV	CCTTC	1 cut(s)	65
HpyCH4III	ACNGT	3 cut(s)	567, 1063, 1211
HpyCH4IV	ACGT	5 cut(s)	92, 185, 258, 907, 1312
HpyF10VI	GCNNNNNNNGC	5 cut(s)	246, 460, 514, 749, 1117
HpyF3I	CTNAG	3 cut(s)	429, 603, 676
HpySE526I	ACGT	5 cut(s)	92, 185, 258, 907, 1312
Hsp92II	CATG	4 cut(s)	116, 208, 467, 772
HspAI	GCGC	3 cut(s)	120, 249, 808
Kpn2I	TCCGGA	2 cut(s)	11, 886
Kzo9I	GATC	5 cut(s)	235, 475, 877, 986, 1132
LmnI	GCTCC	5 cut(s)	14, 127, 835, 1174, 1300
Lsp1109I	GCAGC	5 cut(s)	304, 595, 736, 1268, 1340
LweI	GCATC	4 cut(s)	403, 425, 580, 1286
MaeI	CTAG	1 cut(s)	351
MaeII	ACGT	5 cut(s)	92, 185, 258, 907, 1312
MaeIII	GTNAC	1 cut(s)	979
MalI	GATC	5 cut(s)	237, 477, 879, 988, 1134
MboI	GATC	5 cut(s)	235, 475, 877, 986, 1132
MboII	GAAGA	4 cut(s)	87, 231, 1173, 1272
MfeI	CAATTG	2 cut(s)	155, 485
MhlI	GDGCHC	3 cut(s)	11, 716, 1179
MlsI	TGGCCA	1 cut(s)	203
MluCI	AATT	9 cut(s)	155, 457, 485, 628, 648, 722, 824, 866, 1169
MluNI	TGGCCA	1 cut(s)	203
MlyI	GAGTC	4 cut(s)	24, 167, 440, 689
MmeI	TCCRAC	3 cut(s)	83, 192, 289
Mox20I	TGGCCA	1 cut(s)	203
Mph1103I	ATGCAT	1 cut(s)	418
MroI	TCCGGA	2 cut(s)	11, 886
MroXI	GAANNNNTTC	1 cut(s)	723
MscI	TGGCCA	1 cut(s)	203
MseI	TTAA	4 cut(s)	504, 758, 861, 1008
MslI	CAYNNNNRTG	2 cut(s)	339, 802
Msp20I	TGGCCA	1 cut(s)	203
MspA1I	CMGCKG	3 cut(s)	428, 973, 1259
MspI	CCGG	6 cut(s)	12, 125, 633, 887, 1034, 1298
MspR9I	CCNGG	1 cut(s)	276
MunI	CAATTG	2 cut(s)	155, 485
Mva1269I	GAATGC	4 cut(s)	152, 163, 264, 508
MvaI	CCWGG	1 cut(s)	276
MwoI	GCNNNNNNNGC	5 cut(s)	246, 460, 514, 749, 1117
NdeII	GATC	5 cut(s)	235, 475, 877, 986, 1132
NlaIII	CATG	4 cut(s)	116, 208, 467, 772
NlaIV	GGNNCC	2 cut(s)	482, 1302
NsiI	ATGCAT	1 cut(s)	418
PaeR7I	CTCGAG	1 cut(s)	957
PceI	AGGCCT	1 cut(s)	704
PcsI	WCGNNNNNNNCGW	1 cut(s)	89
PctI	GAATGC	4 cut(s)	152, 163, 264, 508
PdmI	GAANNNNTTC	1 cut(s)	723
PfeI	GAWTC	3 cut(s)	80, 137, 622
PkrI	GCNGC	5 cut(s)	319, 610, 751, 1258, 1330
Ple19I	CGATCG	1 cut(s)	478
PleI	GAGTC	4 cut(s)	23, 167, 439, 689
PpsI	GAGTC	4 cut(s)	23, 167, 439, 689
Ppu21I	YACGTR	1 cut(s)	1313
Psp124BI	GAGCTC	1 cut(s)	1179
Psp6I	CCWGG	1 cut(s)	274
PspGI	CCWGG	1 cut(s)	274
PspN4I	GGNNCC	2 cut(s)	482, 1302
PspPI	GGNCC	1 cut(s)	481
PspXI	VCTCGAGB	1 cut(s)	957
PstI	CTGCAG	1 cut(s)	739
PvuI	CGATCG	1 cut(s)	478
PvuII	CAGCTG	2 cut(s)	973, 1259
RsaI	GTAC	6 cut(s)	33, 107, 227, 383, 767, 1222
RsaNI	GTAC	6 cut(s)	32, 106, 226, 382, 766, 1221
RseI	CAYNNNNRTG	2 cut(s)	339, 802
SacI	GAGCTC	1 cut(s)	1179
SalI	GTCGAC	1 cut(s)	88
SaqAI	TTAA	4 cut(s)	504, 758, 861, 1008
SatI	GCNGC	5 cut(s)	318, 609, 750, 1257, 1329
Sau3AI	GATC	5 cut(s)	235, 475, 877, 986, 1132
Sau96I	GGNCC	1 cut(s)	481
ScaI	AGTACT	3 cut(s)	227, 383, 1222
SchI	GAGTC	4 cut(s)	24, 167, 440, 689
ScrFI	CCNGG	1 cut(s)	276
SduI	GDGCHC	3 cut(s)	11, 716, 1179
SfaNI	GCATC	4 cut(s)	403, 425, 580, 1286
SfcI	CTRYAG	1 cut(s)	735
Sfr274I	CTCGAG	1 cut(s)	957
SgrDI	CGTCGACG	1 cut(s)	88
SlaI	CTCGAG	1 cut(s)	957
SmiMI	CAYNNNNRTG	2 cut(s)	339, 802
SmlI	CTYRAG	1 cut(s)	957
SmoI	CTYRAG	1 cut(s)	957
Sse9I	AATT	9 cut(s)	155, 457, 485, 628, 648, 722, 824, 866, 1169
SseBI	AGGCCT	1 cut(s)	704
SsiI	CCGC	2 cut(s)	128, 426
SspI	AATATT	1 cut(s)	688
SspMI	CTAG	1 cut(s)	351
SstI	GAGCTC	1 cut(s)	1179
StuI	AGGCCT	1 cut(s)	704
StyD4I	CCNGG	1 cut(s)	274
TaaI	ACNGT	3 cut(s)	567, 1063, 1211
TaiI	ACGT	5 cut(s)	95, 188, 261, 910, 1315
TaqI	TCGA	9 cut(s)	89, 217, 255, 411, 474, 546, 802, 958, 1245
TasI	AATT	9 cut(s)	155, 457, 485, 628, 648, 722, 824, 866, 1169
TatI	WGTACW	4 cut(s)	225, 381, 765, 1220
TfiI	GAWTC	3 cut(s)	80, 137, 622
Tru1I	TTAA	4 cut(s)	504, 758, 861, 1008
Tru9I	TTAA	4 cut(s)	504, 758, 861, 1008
TscAI	CASTG	1 cut(s)	570
TseI	GCWGC	5 cut(s)	317, 608, 749, 1256, 1328
TspDTI	ATGAA	2 cut(s)	915, 1182
TspGWI	ACGGA	3 cut(s)	87, 851, 1110
TspRI	CASTG	1 cut(s)	570
XapI	RAATTY	2 cut(s)	628, 722
XcmI	CCANNNNNNNNNTGG	1 cut(s)	373
XhoI	CTCGAG	1 cut(s)	957
XmiI	GTMKAC	1 cut(s)	89
XmnI	GAANNNNTTC	1 cut(s)	723
XspI	CTAG	1 cut(s)	351
ZrmI	AGTACT	3 cut(s)	227, 383, 1222
Zsp2I	ATGCAT	1 cut(s)	418

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.