Serine/threonine phosphatases, family 2C, catalytic domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 2 cut(s) | 2444, 2609 |
| Acc36I | ACCTGC | 1 cut(s) | 1608 |
| AccI | GTMKAC | 1 cut(s) | 2935 |
| AccII | CGCG | 2 cut(s) | 58, 187 |
| AclWI | GGATC | 7 cut(s) | 293, 756, 1417, 1798, 1858, 2821, 3041 |
| AcsI | RAATTY | 9 cut(s) | 582, 629, 755, 1801, 2071, 2183, 2574, 2590, 3017 |
| AcuI | CTGAAG | 5 cut(s) | 360, 423, 1893, 2733, 3138 |
| AcvI | CACGTG | 1 cut(s) | 2849 |
| AcyI | GRCGYC | 1 cut(s) | 8 |
| AfaI | GTAC | 8 cut(s) | 95, 1182, 1229, 1255, 1375, 1658, 1827, 2376 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 272, 277, 772, 1606, 1818 |
| AflII | CTTAAG | 1 cut(s) | 3053 |
| AflIII | ACRYGT | 1 cut(s) | 2846 |
| AhlI | ACTAGT | 2 cut(s) | 455, 2837 |
| AjiI | CACGTC | 1 cut(s) | 81 |
| AjnI | CCWGG | 3 cut(s) | 1195, 1605, 2999 |
| AjuI | GAANNNNNNNTTGG | 8 cut(s) | 353, 385, 510, 542, 1992, 2024, 2157, 2189 |
| AleI | CACNNNNGTG | 1 cut(s) | 1638 |
| Alw21I | GWGCWC | 1 cut(s) | 990 |
| Alw26I | GTCTC | 2 cut(s) | 720, 1039 |
| Alw44I | GTGCAC | 1 cut(s) | 986 |
| AlwI | GGATC | 7 cut(s) | 293, 756, 1417, 1798, 1858, 2821, 3041 |
| Ama87I | CYCGRG | 3 cut(s) | 765, 1017, 2256 |
| AoxI | GGCC | 3 cut(s) | 499, 1971, 2107 |
| ApaLI | GTGCAC | 1 cut(s) | 986 |
| ApeKI | GCWGC | 3 cut(s) | 1118, 1289, 2060 |
| ApoI | RAATTY | 9 cut(s) | 582, 629, 755, 1801, 2071, 2183, 2574, 2590, 3017 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1619, 1651, 2431, 2463 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 365, 756, 1758, 1906, 2019 |
| AspLEI | GCGC | 6 cut(s) | 60, 110, 2445, 2610, 2854, 3096 |
| AspS9I | GGNCC | 5 cut(s) | 222, 232, 1971, 2672, 3112 |
| AsuC2I | CCSGG | 4 cut(s) | 273, 766, 767, 2946 |
| AsuII | TTCGAA | 2 cut(s) | 359, 2250 |
| AsuNHI | GCTAGC | 1 cut(s) | 2899 |
| AvaI | CYCGRG | 3 cut(s) | 765, 1017, 2256 |
| AvaII | GGWCC | 4 cut(s) | 222, 232, 2672, 3112 |
| BaeGI | GKGCMC | 1 cut(s) | 990 |
| BauI | CACGAG | 1 cut(s) | 1923 |
| BbrPI | CACGTG | 1 cut(s) | 2849 |
| BbsI | GAAGAC | 2 cut(s) | 2337, 2420 |
| Bbv12I | GWGCWC | 1 cut(s) | 990 |
| BbvI | GCAGC | 3 cut(s) | 1105, 1301, 2047 |
| BceAI | ACGGC | 1 cut(s) | 32 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1666, 1700 |
| BciT130I | CCWGG | 3 cut(s) | 1197, 1607, 3001 |
| BcnI | CCSGG | 4 cut(s) | 273, 766, 767, 2946 |
| BcoDI | GTCTC | 2 cut(s) | 720, 1039 |
| BcuI | ACTAGT | 2 cut(s) | 455, 2837 |
| BfoI | RGCGCY | 1 cut(s) | 111 |
| BfrI | CTTAAG | 1 cut(s) | 3053 |
| BfuAI | ACCTGC | 1 cut(s) | 1608 |
| BglII | AGATCT | 2 cut(s) | 1207, 2379 |
| BisI | GCNGC | 7 cut(s) | 106, 783, 1119, 1290, 2061, 3086, 3149 |
| BlsI | GCNGC | 7 cut(s) | 107, 784, 1120, 1291, 2062, 3087, 3150 |
| Bme1390I | CCNGG | 7 cut(s) | 273, 766, 767, 1197, 1607, 2946, 3001 |
| Bme18I | GGWCC | 4 cut(s) | 222, 232, 2672, 3112 |
| BmeT110I | CYCGRG | 3 cut(s) | 765, 1017, 2256 |
| BmgBI | CACGTC | 1 cut(s) | 81 |
| BmgT120I | GGNCC | 5 cut(s) | 222, 232, 1971, 2672, 3112 |
| BmiI | GGNNCC | 5 cut(s) | 670, 1972, 2665, 3113, 3114 |
| BmrFI | CCNGG | 7 cut(s) | 273, 766, 767, 1197, 1607, 2946, 3001 |
| BmrI | ACTGGG | 1 cut(s) | 2565 |
| BmtI | GCTAGC | 1 cut(s) | 2903 |
| BmuI | ACTGGG | 1 cut(s) | 2565 |
| BpiI | GAAGAC | 2 cut(s) | 2337, 2420 |
| Bpu14I | TTCGAA | 2 cut(s) | 359, 2250 |
| BpuEI | CTTGAG | 1 cut(s) | 1900 |
| BpuMI | CCSGG | 4 cut(s) | 273, 766, 767, 2946 |
| BsaAI | YACGTR | 2 cut(s) | 1997, 2849 |
| BsaBI | GATNNNNATC | 5 cut(s) | 570, 915, 1107, 1932, 2736 |
| BsaHI | GRCGYC | 1 cut(s) | 8 |
| BsaJI | CCNNGG | 8 cut(s) | 225, 271, 765, 1196, 1605, 1684, 1894, 2944 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1544, 1574, 2770, 2800 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 272, 277, 772, 1606, 1818 |
| Bse1I | ACTGG | 6 cut(s) | 510, 1460, 1734, 1780, 2560, 2674 |
| Bse3DI | GCAATG | 1 cut(s) | 2637 |
| Bse8I | GATNNNNATC | 5 cut(s) | 570, 915, 1107, 1932, 2736 |
| BseBI | CCWGG | 3 cut(s) | 1197, 1607, 3001 |
| BseDI | CCNNGG | 8 cut(s) | 225, 271, 765, 1196, 1605, 1684, 1894, 2944 |
| BseJI | GATNNNNATC | 5 cut(s) | 570, 915, 1107, 1932, 2736 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 272, 277, 772, 1606, 1818 |
| BseMI | GCAATG | 1 cut(s) | 2637 |
| BseMII | CTCAG | 2 cut(s) | 762, 1438 |
| BseNI | ACTGG | 6 cut(s) | 510, 1460, 1734, 1780, 2560, 2674 |
| BseRI | GAGGAG | 2 cut(s) | 2697, 3162 |
| BseSI | GKGCMC | 1 cut(s) | 990 |
| BseXI | GCAGC | 3 cut(s) | 1105, 1301, 2047 |
| BsgI | GTGCAG | 1 cut(s) | 1047 |
| Bsh1236I | CGCG | 2 cut(s) | 58, 187 |
| BshFI | GGCC | 3 cut(s) | 501, 1973, 2109 |
| BsiHKAI | GWGCWC | 1 cut(s) | 990 |
| BsiHKCI | CYCGRG | 3 cut(s) | 765, 1017, 2256 |
| BsiSI | CCGG | 5 cut(s) | 113, 217, 273, 766, 2946 |
| BslFI | GGGAC | 1 cut(s) | 3125 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 272, 277, 772, 1606, 1818 |
| BsmAI | GTCTC | 2 cut(s) | 720, 1039 |
| BsmFI | GGGAC | 1 cut(s) | 3125 |
| BsmI | GAATGC | 2 cut(s) | 261, 2344 |
| BsnI | GGCC | 3 cut(s) | 501, 1973, 2109 |
| BsoBI | CYCGRG | 3 cut(s) | 765, 1017, 2256 |
| Bsp119I | TTCGAA | 2 cut(s) | 359, 2250 |
| Bsp1286I | GDGCHC | 1 cut(s) | 990 |
| Bsp1407I | TGTACA | 2 cut(s) | 93, 2374 |
| Bsp19I | CCATGG | 1 cut(s) | 225 |
| BspANI | GGCC | 3 cut(s) | 501, 1973, 2109 |
| BspCNI | CTCAG | 2 cut(s) | 761, 1437 |
| BspFNI | CGCG | 2 cut(s) | 58, 187 |
| BspHI | TCATGA | 2 cut(s) | 613, 2485 |
| BspLI | GGNNCC | 5 cut(s) | 670, 1972, 2665, 3113, 3114 |
| BspMI | ACCTGC | 1 cut(s) | 1608 |
| BspOI | GCTAGC | 1 cut(s) | 2903 |
| BspPI | GGATC | 7 cut(s) | 293, 756, 1417, 1798, 1858, 2821, 3041 |
| BspQI | GCTCTTC | 2 cut(s) | 1617, 2538 |
| BspT104I | TTCGAA | 2 cut(s) | 359, 2250 |
| BspTI | CTTAAG | 1 cut(s) | 3053 |
| BsrDI | GCAATG | 1 cut(s) | 2637 |
| BsrGI | TGTACA | 2 cut(s) | 93, 2374 |
| BsrI | ACTGG | 6 cut(s) | 510, 1460, 1734, 1780, 2560, 2674 |
| BssECI | CCNNGG | 8 cut(s) | 225, 271, 765, 1196, 1605, 1684, 1894, 2944 |
| BssNAI | GTATAC | 1 cut(s) | 2936 |
| BssNI | GRCGYC | 1 cut(s) | 8 |
| BssSI | CACGAG | 1 cut(s) | 1923 |
| BssT1I | CCWWGG | 1 cut(s) | 225 |
| Bst1107I | GTATAC | 1 cut(s) | 2936 |
| Bst2BI | CACGAG | 1 cut(s) | 1923 |
| Bst2UI | CCWGG | 3 cut(s) | 1197, 1607, 3001 |
| Bst6I | CTCTTC | 3 cut(s) | 1617, 1829, 2538 |
| BstACI | GRCGYC | 1 cut(s) | 8 |
| BstAFI | CTTAAG | 1 cut(s) | 3053 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 243, 692 |
| BstAUI | TGTACA | 2 cut(s) | 93, 2374 |
| BstBAI | YACGTR | 2 cut(s) | 1997, 2849 |
| BstBI | TTCGAA | 2 cut(s) | 359, 2250 |
| BstC8I | GCNNGC | 4 cut(s) | 1679, 1872, 2901, 3098 |
| BstDSI | CCRYGG | 1 cut(s) | 225 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1604 |
| BstFNI | CGCG | 2 cut(s) | 58, 187 |
| BstH2I | RGCGCY | 1 cut(s) | 111 |
| BstHHI | GCGC | 6 cut(s) | 60, 110, 2445, 2610, 2854, 3096 |
| BstMAI | GTCTC | 2 cut(s) | 720, 1039 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 243, 683, 692, 782, 1512, 1725, 2993, 3091 |
| BstNI | CCWGG | 3 cut(s) | 1197, 1607, 3001 |
| BstNSI | RCATGY | 4 cut(s) | 1550, 1622, 1681, 2375 |
| BstSCI | CCNGG | 7 cut(s) | 271, 764, 765, 1195, 1605, 2944, 2999 |
| BstSLI | GKGCMC | 1 cut(s) | 990 |
| BstUI | CGCG | 2 cut(s) | 58, 187 |
| BstV1I | GCAGC | 3 cut(s) | 1105, 1301, 2047 |
| BstV2I | GAAGAC | 2 cut(s) | 2337, 2420 |
| BstX2I | RGATCY | 4 cut(s) | 1207, 1850, 2379, 3046 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 1637, 2397, 3000, 3075 |
| BstYI | RGATCY | 4 cut(s) | 1207, 1850, 2379, 3046 |
| BstZ17I | GTATAC | 1 cut(s) | 2936 |
| BsuRI | GGCC | 3 cut(s) | 501, 1973, 2109 |
| BtgI | CCRYGG | 1 cut(s) | 225 |
| BtgZI | GCGATG | 1 cut(s) | 215 |
| BtrI | CACGTC | 1 cut(s) | 81 |
| BtsIMutI | CAGTG | 3 cut(s) | 1974, 2553, 2667 |
| BveI | ACCTGC | 1 cut(s) | 1608 |
| Cac8I | GCNNGC | 4 cut(s) | 1679, 1872, 2901, 3098 |
| CciI | TCATGA | 2 cut(s) | 613, 2485 |
| CfoI | GCGC | 6 cut(s) | 60, 110, 2445, 2610, 2854, 3096 |
| Cfr13I | GGNCC | 5 cut(s) | 222, 232, 1971, 2672, 3112 |
| Cfr9I | CCCGGG | 1 cut(s) | 765 |
| CseI | GACGC | 3 cut(s) | 64, 1110, 2375 |
| Csp6I | GTAC | 8 cut(s) | 94, 1181, 1228, 1254, 1374, 1657, 1826, 2375 |
| CviQI | GTAC | 8 cut(s) | 94, 1181, 1228, 1254, 1374, 1657, 1826, 2375 |
| DraI | TTTAAA | 1 cut(s) | 1042 |
| Eam1104I | CTCTTC | 3 cut(s) | 1617, 1829, 2538 |
| EarI | CTCTTC | 3 cut(s) | 1617, 1829, 2538 |
| Eco130I | CCWWGG | 1 cut(s) | 225 |
| Eco32I | GATATC | 1 cut(s) | 2512 |
| Eco47I | GGWCC | 4 cut(s) | 222, 232, 2672, 3112 |
| Eco57I | CTGAAG | 5 cut(s) | 360, 423, 1893, 2733, 3138 |
| Eco72I | CACGTG | 1 cut(s) | 2849 |
| Eco88I | CYCGRG | 3 cut(s) | 765, 1017, 2256 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1604 |
| EcoO109I | RGGNCCY | 1 cut(s) | 3112 |
| EcoRI | GAATTC | 2 cut(s) | 1801, 2574 |
| EcoRII | CCWGG | 3 cut(s) | 1195, 1605, 2999 |
| EcoRV | GATATC | 1 cut(s) | 2512 |
| EcoT14I | CCWWGG | 1 cut(s) | 225 |
| EcoT22I | ATGCAT | 4 cut(s) | 412, 885, 1721, 2032 |
| ErhI | CCWWGG | 1 cut(s) | 225 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 353, 385, 2007, 2039, 2706, 2738 |
| FaqI | GGGAC | 1 cut(s) | 3125 |
| FblI | GTMKAC | 1 cut(s) | 2935 |
| Fnu4HI | GCNGC | 7 cut(s) | 106, 783, 1119, 1290, 2061, 3086, 3149 |
| Fsp4HI | GCNGC | 7 cut(s) | 106, 783, 1119, 1290, 2061, 3086, 3149 |
| FspAI | RTGCGCAY | 1 cut(s) | 2609 |
| FspI | TGCGCA | 2 cut(s) | 2444, 2609 |
| GlaI | GCGC | 6 cut(s) | 59, 109, 2444, 2609, 2853, 3095 |
| GluI | GCNGC | 7 cut(s) | 106, 783, 1119, 1290, 2061, 3086, 3149 |
| HaeII | RGCGCY | 1 cut(s) | 111 |
| HaeIII | GGCC | 3 cut(s) | 501, 1973, 2109 |
| HapII | CCGG | 5 cut(s) | 113, 217, 273, 766, 2946 |
| HgaI | GACGC | 3 cut(s) | 64, 1110, 2375 |
| HhaI | GCGC | 6 cut(s) | 60, 110, 2445, 2610, 2854, 3096 |
| Hin1I | GRCGYC | 1 cut(s) | 8 |
| Hin6I | GCGC | 6 cut(s) | 58, 108, 2443, 2608, 2852, 3094 |
| HinP1I | GCGC | 6 cut(s) | 58, 108, 2443, 2608, 2852, 3094 |
| HincII | GTYRAC | 2 cut(s) | 12, 1398 |
| HindII | GTYRAC | 2 cut(s) | 12, 1398 |
| HindIII | AAGCTT | 5 cut(s) | 362, 435, 2167, 2309, 2909 |
| HpaII | CCGG | 5 cut(s) | 113, 217, 273, 766, 2946 |
| Hpy99I | CGWCG | 2 cut(s) | 58, 82 |
| HpyCH4IV | ACGT | 5 cut(s) | 80, 1284, 1400, 1996, 2848 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 243, 683, 692, 782, 1512, 1725, 2993, 3091 |
| HpySE526I | ACGT | 5 cut(s) | 80, 1284, 1400, 1996, 2848 |
| Hsp92I | GRCGYC | 1 cut(s) | 8 |
| HspAI | GCGC | 6 cut(s) | 58, 108, 2443, 2608, 2852, 3094 |
| KflI | GGGWCCC | 1 cut(s) | 3112 |
| LguI | GCTCTTC | 2 cut(s) | 1617, 2538 |
| LmnI | GCTCC | 6 cut(s) | 115, 335, 2434, 2896, 2926, 2984 |
| Lsp1109I | GCAGC | 3 cut(s) | 1105, 1301, 2047 |
| MaeII | ACGT | 5 cut(s) | 80, 1284, 1400, 1996, 2848 |
| MaeIII | GTNAC | 5 cut(s) | 295, 1054, 1427, 1889, 2840 |
| MfeI | CAATTG | 1 cut(s) | 1442 |
| MflI | RGATCY | 4 cut(s) | 1207, 1850, 2379, 3046 |
| MhlI | GDGCHC | 1 cut(s) | 990 |
| MlyI | GAGTC | 3 cut(s) | 722, 870, 1907 |
| MmeI | TCCRAC | 4 cut(s) | 283, 1862, 2135, 2962 |
| Mph1103I | ATGCAT | 4 cut(s) | 412, 885, 1721, 2032 |
| MroXI | GAANNNNTTC | 5 cut(s) | 365, 756, 1758, 1906, 2019 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1506, 1638, 2395, 2603, 2658 |
| MspA1I | CMGCKG | 3 cut(s) | 872, 1292, 2060 |
| MspCI | CTTAAG | 1 cut(s) | 3053 |
| MspI | CCGG | 5 cut(s) | 113, 217, 273, 766, 2946 |
| MspR9I | CCNGG | 7 cut(s) | 273, 766, 767, 1197, 1607, 2946, 3001 |
| MunI | CAATTG | 1 cut(s) | 1442 |
| Mva1269I | GAATGC | 2 cut(s) | 261, 2344 |
| MvaI | CCWGG | 3 cut(s) | 1197, 1607, 3001 |
| MvnI | CGCG | 2 cut(s) | 58, 187 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 243, 683, 692, 782, 1512, 1725, 2993, 3091 |
| NciI | CCSGG | 4 cut(s) | 273, 766, 767, 2946 |
| NcoI | CCATGG | 1 cut(s) | 225 |
| NheI | GCTAGC | 1 cut(s) | 2899 |
| NlaIV | GGNNCC | 5 cut(s) | 670, 1972, 2665, 3113, 3114 |
| NmuCI | GTSAC | 2 cut(s) | 295, 1889 |
| NsbI | TGCGCA | 2 cut(s) | 2444, 2609 |
| NsiI | ATGCAT | 4 cut(s) | 412, 885, 1721, 2032 |
| NspI | RCATGY | 4 cut(s) | 1550, 1622, 1681, 2375 |
| NspV | TTCGAA | 2 cut(s) | 359, 2250 |
| OliI | CACNNNNGTG | 1 cut(s) | 1638 |
| PaeI | GCATGC | 1 cut(s) | 1681 |
| PagI | TCATGA | 2 cut(s) | 613, 2485 |
| PciSI | GCTCTTC | 2 cut(s) | 1617, 2538 |
| PctI | GAATGC | 2 cut(s) | 261, 2344 |
| PdmI | GAANNNNTTC | 5 cut(s) | 365, 756, 1758, 1906, 2019 |
| PfoI | TCCNGGA | 1 cut(s) | 2999 |
| PkrI | GCNGC | 7 cut(s) | 107, 784, 1120, 1291, 2062, 3087, 3150 |
| PleI | GAGTC | 3 cut(s) | 722, 870, 1907 |
| PmaCI | CACGTG | 1 cut(s) | 2849 |
| PmlI | CACGTG | 1 cut(s) | 2849 |
| PpsI | GAGTC | 3 cut(s) | 722, 870, 1907 |
| Ppu21I | YACGTR | 2 cut(s) | 1997, 2849 |
| PpuMI | RGGWCCY | 1 cut(s) | 3112 |
| Psp5II | RGGWCCY | 1 cut(s) | 3112 |
| Psp6I | CCWGG | 3 cut(s) | 1195, 1605, 2999 |
| PspCI | CACGTG | 1 cut(s) | 2849 |
| PspGI | CCWGG | 3 cut(s) | 1195, 1605, 2999 |
| PspN4I | GGNNCC | 5 cut(s) | 670, 1972, 2665, 3113, 3114 |
| PspPI | GGNCC | 5 cut(s) | 222, 232, 1971, 2672, 3112 |
| PspPPI | RGGWCCY | 1 cut(s) | 3112 |
| PsuI | RGATCY | 4 cut(s) | 1207, 1850, 2379, 3046 |
| PvuII | CAGCTG | 2 cut(s) | 1292, 2060 |
| RsaI | GTAC | 8 cut(s) | 95, 1182, 1229, 1255, 1375, 1658, 1827, 2376 |
| RsaNI | GTAC | 8 cut(s) | 94, 1181, 1228, 1254, 1374, 1657, 1826, 2375 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1506, 1638, 2395, 2603, 2658 |
| SapI | GCTCTTC | 2 cut(s) | 1617, 2538 |
| SatI | GCNGC | 7 cut(s) | 106, 783, 1119, 1290, 2061, 3086, 3149 |
| Sau96I | GGNCC | 5 cut(s) | 222, 232, 1971, 2672, 3112 |
| SchI | GAGTC | 3 cut(s) | 722, 870, 1907 |
| ScrFI | CCNGG | 7 cut(s) | 273, 766, 767, 1197, 1607, 2946, 3001 |
| SduI | GDGCHC | 1 cut(s) | 990 |
| SfuI | TTCGAA | 2 cut(s) | 359, 2250 |
| SinI | GGWCC | 4 cut(s) | 222, 232, 2672, 3112 |
| SmaI | CCCGGG | 1 cut(s) | 767 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1506, 1638, 2395, 2603, 2658 |
| SmlI | CTYRAG | 2 cut(s) | 1915, 3053 |
| SmoI | CTYRAG | 2 cut(s) | 1915, 3053 |
| SpeI | ACTAGT | 2 cut(s) | 455, 2837 |
| SphI | GCATGC | 1 cut(s) | 1681 |
| SspI | AATATT | 2 cut(s) | 721, 2497 |
| StyD4I | CCNGG | 7 cut(s) | 271, 764, 765, 1195, 1605, 2944, 2999 |
| StyI | CCWWGG | 1 cut(s) | 225 |
| TaiI | ACGT | 5 cut(s) | 83, 1287, 1403, 1999, 2851 |
| TaqII | GACCGA | 2 cut(s) | 1908, 2991 |
| TatI | WGTACW | 4 cut(s) | 93, 1227, 1825, 2374 |
| TauI | GCSGC | 4 cut(s) | 108, 785, 3088, 3151 |
| TscAI | CASTG | 3 cut(s) | 1981, 2560, 2674 |
| TseFI | GTSAC | 2 cut(s) | 295, 1889 |
| TseI | GCWGC | 3 cut(s) | 1118, 1289, 2060 |
| Tsp45I | GTSAC | 2 cut(s) | 295, 1889 |
| TspGWI | ACGGA | 3 cut(s) | 1091, 1966, 2177 |
| TspMI | CCCGGG | 1 cut(s) | 765 |
| TspRI | CASTG | 3 cut(s) | 1981, 2560, 2674 |
| Vha464I | CTTAAG | 1 cut(s) | 3053 |
| VneI | GTGCAC | 1 cut(s) | 986 |
| VpaK11BI | GGWCC | 4 cut(s) | 222, 232, 2672, 3112 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1604 |
| XapI | RAATTY | 9 cut(s) | 582, 629, 755, 1801, 2071, 2183, 2574, 2590, 3017 |
| XceI | RCATGY | 4 cut(s) | 1550, 1622, 1681, 2375 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 851, 1637, 2397 |
| XmaI | CCCGGG | 1 cut(s) | 765 |
| XmiI | GTMKAC | 1 cut(s) | 2935 |
| XmnI | GAANNNNTTC | 5 cut(s) | 365, 756, 1758, 1906, 2019 |
| Zsp2I | ATGCAT | 4 cut(s) | 412, 885, 1721, 2032 |