Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0000935

Physical Location & Seq

Genomic Coordinates Forward (+)

32226108 .. 32228271

Gene Structure 2164 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1147800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1197 bp

ATGGAGTGCTCCGGAGTCCTCCACCTATTTGTACTGGGATTACTATTCACCAGCCTTCCGACGCTGTCCTACGGAGGAGAATCGTCGTCGACGTGCTTGGCGCTGTACGAACATGGTGGAGCTCCGGCGATTTTTGAATCCCCAGAATGCCGTCAATTGAAGCACTCTGACGACTCCCGACGCACGTCGCTTTGTCAAACAACCATGCTTCAAGGTCGGAGAAAGTACTTGGAGGATCGCACTCTCTGCGCTCTCAACCTTCGCATTCCCTTTCCTGGTAGAGTAGGGATTATGGAAAAGATTAGAGTTGGAGTTGCTGCTGTTTTTTATGGTCATAATGGTGCTGAAGCTAGTGACATGGCATCCAAGCTTTTCTTGGAGTATTTCGTTTTGCATACCTACTTTCTTCTCGATGTATCGCTTTCCGCTGCATCATCTGGAGCACCAACATTGCAAAATTGGCATGGATTACTCAATCGGCACCAATTGGATAATGCAAGGTTTAAGCATTCGTTGCAAGCAAACTTTGACGATTCTTTTCGCTTTGACATCTTAAAAAAAACATTGTTGAGGGCAGTTCATGATATTAATGCAAAATTTTCTAAGACTCCTGTAGAGGCTAAAGCTGCTTATTTAAGATTGTACATGGAGGAAAGGCGTAATGGTGTTATCTCACATCGAAGGCGCAATAAAAACTTTGAATTAGCTCCCGCGGATGGGTTAGTGCATTTATCTGTTAAGGAATTGACAAAAGATCATCATCCAGACATAGACGATGAAAAACTTAGGGTGGAAAATGCTGGTGGAAATGTTCTTGAATGGGCTGGCTTGCTGTTGACTGTCATCAGTTTTGGCGTTGTATCAGAACCGGAGTTGACAGATTGGCAACCCCTGACTGTAAATGATACATATCTCGTGGCTACATCTGACGGAGTTTTTGAGAAGCAGAGCTTGCAGGATGTTTGCGATCTGTTGTGGGAAGCACGAAGTCGTCATCCTACAAAATTGGAGCTCTCTTCAGCTGGTTCATACTCATTAGCTGACTGCATTGTAAGTACTGCATTTGAAAAGGGCAGTATGGATAATATGGCAGCTGTTGTAGTTCCATTGGCATATTTTCGTTTTTCTCAACCGGAGCCATTGCTACGTGTTTTCTCATTTGCAGCTAACAACTCCTCTTTAGTTCATACTGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C Pkinase

Protein Analysis

399

Amino Acids

44.36

Weight (kDa)

6.31

Isoelectric Point (pI)

45.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	247 - 362	3.8e-07	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000935)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63320 AT3G63320 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340 AT3G63340
fragaria_vesca	FvH4_2g23010 FvH4_2g23010 FvH4_2g23010 FvH4_2g23010 FvH4_2g23010
malus_domestica	MD00G1147300.v1.1 MD00G1147600.v1.1 MD00G1147700.v1.1 MD00G1147800.v1.1 MD03G1258500.v1.1 MD08G1204000.v1.1
prunus_persica	Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1 Prupe.8G235000_v2.0.a1
pyrus_communis	pycom03g20470 pycom08g17600 pycom08g17610 pycom08g17640 pycom08g17650 pycom08g17660
rosa_chinensis	RchiOBHm_Chr3g0479641 RchiOBHm_Chr6g0290051
rosa_laevigata	RLG00000012250 RLG00000023551 RLG00000023553 RLG00000023556 RLG00000029889
rosa_multiflora	Rmu_co8325477.1_g000001 Rmu_sc0001775.1_g000013 Rmu_sc0002902.1_g000039
rosa_roxburghii	Rroxscaffold_6G00402100 Rroxscaffold_6G00402120 Rroxscaffold_7G00177390
rosa_rugosa	Rorug03G0176900 Rorug03G0177000 Rorug06G0209000 Rorug06G0209100
rosa_samantha	Rh3AG227700 Rh3BG260800 Rh3BG261000 Rh3CG258100 Rh3DG254200 Rh6AG320000 Rh6BG327200 Rh6CG332900 Rh6DG318800
rosa_wichuraiana	Rw3G020380 Rw6G027610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	480
AccI	GTMKAC	1 cut(s)	89
AccII	CGCG	1 cut(s)	713
AccIII	TCCGGA	1 cut(s)	11
AciI	CCGC	3 cut(s)	426, 711, 713
AclWI	GGATC	1 cut(s)	243
AcsI	RAATTY	1 cut(s)	596
AcuI	CTGAAG	2 cut(s)	366, 1002
AfaI	GTAC	5 cut(s)	33, 107, 227, 644, 1057
AfiI	CCNNNNNNNGG	3 cut(s)	275, 716, 717
AflIII	ACRYGT	1 cut(s)	1147
AgsI	TTSAA	6 cut(s)	137, 160, 212, 701, 818, 1067
AjiI	CACGTC	2 cut(s)	93, 186
AjnI	CCWGG	1 cut(s)	274
Alw21I	GWGCWC	4 cut(s)	11, 124, 445, 1014
AlwI	GGATC	1 cut(s)	243
Aor13HI	TCCGGA	1 cut(s)	11
ApeKI	GCWGC	5 cut(s)	317, 428, 626, 1091, 1163
ApoI	RAATTY	1 cut(s)	596
ArsI	GACNNNNNNTTYG	2 cut(s)	920, 952
AseI	ATTAAT	1 cut(s)	588
AspLEI	GCGC	3 cut(s)	103, 251, 687
AsuHPI	GGTGA	1 cut(s)	40
BanI	GGYRCC	1 cut(s)	480
BanII	GRGCYC	2 cut(s)	124, 1014
BauI	CACGAG	1 cut(s)	914
Bbv12I	GWGCWC	4 cut(s)	11, 124, 445, 1014
BbvI	GCAGC	5 cut(s)	304, 415, 613, 1103, 1175
BccI	CCATC	1 cut(s)	710
BceAI	ACGGC	1 cut(s)	135
BciT130I	CCWGG	1 cut(s)	276
BfaI	CTAG	1 cut(s)	351
BfmI	CTRYAG	1 cut(s)	612
BfoI	RGCGCY	1 cut(s)	104
BisI	GCNGC	5 cut(s)	318, 429, 627, 1092, 1164
BlsI	GCNGC	5 cut(s)	319, 430, 628, 1093, 1165
BmcAI	AGTACT	2 cut(s)	227, 1057
Bme1390I	CCNGG	1 cut(s)	276
BmgBI	CACGTC	2 cut(s)	93, 186
BmiI	GGNNCC	2 cut(s)	482, 1137
BmrFI	CCNGG	1 cut(s)	276
BmrI	ACTGGG	1 cut(s)	44
BmsI	GCATC	2 cut(s)	371, 440
BmuI	ACTGGG	1 cut(s)	44
BoxI	GACNNNNGTC	1 cut(s)	184
BpmI	CTGGAG	1 cut(s)	459
BsaAI	YACGTR	1 cut(s)	1148
BsaBI	GATNNNNATC	2 cut(s)	759, 909
BsaJI	CCNNGG	1 cut(s)	711
BsaWI	WCCGGW	3 cut(s)	11, 868, 1132
Bsc4I	CCNNNNNNNGG	3 cut(s)	275, 716, 717
Bse1I	ACTGG	2 cut(s)	39, 1195
Bse3DI	GCAATG	2 cut(s)	449, 1139
Bse8I	GATNNNNATC	2 cut(s)	759, 909
BseAI	TCCGGA	1 cut(s)	11
BseBI	CCWGG	1 cut(s)	276
BseDI	CCNNGG	1 cut(s)	711
BseGI	GGATG	5 cut(s)	362, 721, 760, 964, 994
BseJI	GATNNNNATC	2 cut(s)	759, 909
BseLI	CCNNNNNNNGG	3 cut(s)	275, 716, 717
BseMI	GCAATG	2 cut(s)	449, 1139
BseNI	ACTGG	2 cut(s)	39, 1195
BseRI	GAGGAG	2 cut(s)	90, 1165
BseXI	GCAGC	5 cut(s)	304, 415, 613, 1103, 1175
Bsh1236I	CGCG	1 cut(s)	713
BshNI	GGYRCC	1 cut(s)	480
BsiHKAI	GWGCWC	4 cut(s)	11, 124, 445, 1014
BsiSI	CCGG	4 cut(s)	12, 125, 869, 1133
BslI	CCNNNNNNNGG	3 cut(s)	275, 716, 717
BsmI	GAATGC	3 cut(s)	152, 264, 508
Bsp1286I	GDGCHC	4 cut(s)	11, 124, 445, 1014
Bsp13I	TCCGGA	1 cut(s)	11
Bsp1407I	TGTACA	1 cut(s)	642
Bsp143I	GATC	3 cut(s)	235, 754, 967
BspACI	CCGC	3 cut(s)	426, 711, 713
BspEI	TCCGGA	1 cut(s)	11
BspFNI	CGCG	1 cut(s)	713
BspHI	TCATGA	1 cut(s)	580
BspLI	GGNNCC	2 cut(s)	482, 1137
BspPI	GGATC	1 cut(s)	243
BspT107I	GGYRCC	1 cut(s)	480
BsrDI	GCAATG	2 cut(s)	449, 1139
BsrGI	TGTACA	1 cut(s)	642
BsrI	ACTGG	2 cut(s)	39, 1195
BssECI	CCNNGG	1 cut(s)	711
BssMI	GATC	3 cut(s)	235, 754, 967
BssSI	CACGAG	1 cut(s)	914
Bst2BI	CACGAG	1 cut(s)	914
Bst2UI	CCWGG	1 cut(s)	276
Bst4CI	ACNGT	2 cut(s)	841, 898
Bst6I	CTCTTC	1 cut(s)	1021
BstAPI	GCANNNNNTGC	3 cut(s)	246, 514, 952
BstAUI	TGTACA	1 cut(s)	642
BstBAI	YACGTR	1 cut(s)	1148
BstC8I	GCNNGC	4 cut(s)	519, 826, 830, 953
BstDEI	CTNAG	2 cut(s)	603, 785
BstDSI	CCRYGG	1 cut(s)	711
BstF5I	GGATG	5 cut(s)	362, 721, 760, 964, 994
BstFNI	CGCG	1 cut(s)	713
BstH2I	RGCGCY	1 cut(s)	104
BstHHI	GCGC	3 cut(s)	103, 251, 687
BstKTI	GATC	3 cut(s)	238, 757, 970
BstMBI	GATC	3 cut(s)	235, 754, 967
BstMWI	GCNNNNNNNGC	5 cut(s)	246, 460, 514, 626, 952
BstNI	CCWGG	1 cut(s)	276
BstPAI	GACNNNNGTC	1 cut(s)	184
BstSCI	CCNGG	1 cut(s)	274
BstSFI	CTRYAG	1 cut(s)	612
BstUI	CGCG	1 cut(s)	713
BstV1I	GCAGC	5 cut(s)	304, 415, 613, 1103, 1175
BtgI	CCRYGG	1 cut(s)	711
BtrI	CACGTC	2 cut(s)	93, 186
BtsCI	GGATG	5 cut(s)	362, 721, 760, 964, 994
Cac8I	GCNNGC	4 cut(s)	519, 826, 830, 953
CciI	TCATGA	1 cut(s)	580
CfoI	GCGC	3 cut(s)	103, 251, 687
Cfr42I	CCGCGG	1 cut(s)	714
CseI	GACGC	2 cut(s)	70, 189
Csp6I	GTAC	5 cut(s)	32, 106, 226, 643, 1056
CviAII	CATG	6 cut(s)	113, 205, 358, 464, 581, 646
CviQI	GTAC	5 cut(s)	32, 106, 226, 643, 1056
DdeI	CTNAG	2 cut(s)	603, 785
DpnI	GATC	3 cut(s)	237, 756, 969
DpnII	GATC	3 cut(s)	235, 754, 967
Eam1104I	CTCTTC	1 cut(s)	1021
EarI	CTCTTC	1 cut(s)	1021
Ecl136II	GAGCTC	2 cut(s)	122, 1012
Eco24I	GRGCYC	2 cut(s)	124, 1014
Eco53kI	GAGCTC	2 cut(s)	122, 1012
Eco57I	CTGAAG	2 cut(s)	366, 1002
EcoICRI	GAGCTC	2 cut(s)	122, 1012
EcoRII	CCWGG	1 cut(s)	274
EcoT38I	GRGCYC	2 cut(s)	124, 1014
FaeI	CATG	6 cut(s)	116, 208, 361, 467, 584, 649
FalI	AAGNNNNNCTT	4 cut(s)	359, 391, 935, 967
FatI	CATG	6 cut(s)	112, 204, 357, 463, 580, 645
FauI	CCCGC	1 cut(s)	718
FblI	GTMKAC	1 cut(s)	89
Fnu4HI	GCNGC	5 cut(s)	318, 429, 627, 1092, 1164
FokI	GGATG	5 cut(s)	349, 728, 747, 971, 981
FriOI	GRGCYC	2 cut(s)	124, 1014
Fsp4HI	GCNGC	5 cut(s)	318, 429, 627, 1092, 1164
FspBI	CTAG	1 cut(s)	351
GlaI	GCGC	3 cut(s)	102, 250, 686
GluI	GCNGC	5 cut(s)	318, 429, 627, 1092, 1164
GsuI	CTGGAG	1 cut(s)	459
HaeII	RGCGCY	1 cut(s)	104
HapII	CCGG	4 cut(s)	12, 125, 869, 1133
HgaI	GACGC	2 cut(s)	70, 189
HhaI	GCGC	3 cut(s)	103, 251, 687
Hin1II	CATG	6 cut(s)	116, 208, 361, 467, 584, 649
Hin6I	GCGC	3 cut(s)	101, 249, 685
HinP1I	GCGC	3 cut(s)	101, 249, 685
HincII	GTYRAC	3 cut(s)	90, 837, 876
HindII	GTYRAC	3 cut(s)	90, 837, 876
HindIII	AAGCTT	1 cut(s)	368
HinfI	GANTC	6 cut(s)	15, 80, 137, 173, 533, 607
HpaII	CCGG	4 cut(s)	12, 125, 869, 1133
HphI	GGTGA	1 cut(s)	40
Hpy166II	GTNNAC	3 cut(s)	90, 837, 876
Hpy188I	TCNGA	5 cut(s)	60, 169, 219, 865, 928
Hpy188III	TCNNGA	7 cut(s)	12, 177, 410, 438, 581, 764, 815
Hpy8I	GTNNAC	3 cut(s)	90, 837, 876
Hpy99I	CGWCG	6 cut(s)	64, 88, 91, 94, 183, 190
HpyAV	CCTTC	3 cut(s)	65, 269, 675
HpyCH4III	ACNGT	2 cut(s)	841, 898
HpyCH4IV	ACGT	3 cut(s)	92, 185, 1147
HpyF10VI	GCNNNNNNNGC	5 cut(s)	246, 460, 514, 626, 952
HpyF3I	CTNAG	2 cut(s)	603, 785
HpySE526I	ACGT	3 cut(s)	92, 185, 1147
Hsp92II	CATG	6 cut(s)	116, 208, 361, 467, 584, 649
HspAI	GCGC	3 cut(s)	101, 249, 685
Kpn2I	TCCGGA	1 cut(s)	11
KspI	CCGCGG	1 cut(s)	714
Kzo9I	GATC	3 cut(s)	235, 754, 967
LmnI	GCTCC	7 cut(s)	14, 119, 127, 440, 712, 1009, 1135
Lsp1109I	GCAGC	5 cut(s)	304, 415, 613, 1103, 1175
LweI	GCATC	2 cut(s)	371, 440
MaeI	CTAG	1 cut(s)	351
MaeII	ACGT	3 cut(s)	92, 185, 1147
MaeIII	GTNAC	1 cut(s)	353
MalI	GATC	3 cut(s)	237, 756, 969
MboI	GATC	3 cut(s)	235, 754, 967
MboII	GAAGA	2 cut(s)	398, 1008
MfeI	CAATTG	2 cut(s)	155, 485
MhlI	GDGCHC	4 cut(s)	11, 124, 445, 1014
MluCI	AATT	7 cut(s)	155, 457, 485, 596, 701, 743, 1004
MlyI	GAGTC	3 cut(s)	24, 167, 601
MmeI	TCCRAC	3 cut(s)	83, 197, 289
MnlI	CCTC	7 cut(s)	29, 68, 226, 564, 610, 643, 1186
MroI	TCCGGA	1 cut(s)	11
MseI	TTAA	5 cut(s)	504, 554, 588, 635, 738
MslI	CAYNNNNRTG	1 cut(s)	339
MspA1I	CMGCKG	4 cut(s)	428, 713, 1022, 1094
MspI	CCGG	4 cut(s)	12, 125, 869, 1133
MspR9I	CCNGG	1 cut(s)	276
MunI	CAATTG	2 cut(s)	155, 485
Mva1269I	GAATGC	3 cut(s)	152, 264, 508
MvaI	CCWGG	1 cut(s)	276
MvnI	CGCG	1 cut(s)	713
MwoI	GCNNNNNNNGC	5 cut(s)	246, 460, 514, 626, 952
NdeII	GATC	3 cut(s)	235, 754, 967
NlaIII	CATG	6 cut(s)	116, 208, 361, 467, 584, 649
NlaIV	GGNNCC	2 cut(s)	482, 1137
NmuCI	GTSAC	1 cut(s)	353
PagI	TCATGA	1 cut(s)	580
PcsI	WCGNNNNNNNCGW	1 cut(s)	89
PctI	GAATGC	3 cut(s)	152, 264, 508
PfeI	GAWTC	3 cut(s)	80, 137, 533
PflFI	GACNNNGTC	1 cut(s)	64
PkrI	GCNGC	5 cut(s)	319, 430, 628, 1093, 1165
PleI	GAGTC	3 cut(s)	23, 167, 601
PpsI	GAGTC	3 cut(s)	23, 167, 601
Ppu21I	YACGTR	1 cut(s)	1148
PshAI	GACNNNNGTC	1 cut(s)	184
PshBI	ATTAAT	1 cut(s)	588
Psp124BI	GAGCTC	2 cut(s)	124, 1014
Psp6I	CCWGG	1 cut(s)	274
PspGI	CCWGG	1 cut(s)	274
PspN4I	GGNNCC	2 cut(s)	482, 1137
PsyI	GACNNNGTC	1 cut(s)	64
PvuII	CAGCTG	2 cut(s)	1022, 1094
RsaI	GTAC	5 cut(s)	33, 107, 227, 644, 1057
RsaNI	GTAC	5 cut(s)	32, 106, 226, 643, 1056
RseI	CAYNNNNRTG	1 cut(s)	339
SacI	GAGCTC	2 cut(s)	124, 1014
SacII	CCGCGG	1 cut(s)	714
SalI	GTCGAC	1 cut(s)	88
SaqAI	TTAA	5 cut(s)	504, 554, 588, 635, 738
SatI	GCNGC	5 cut(s)	318, 429, 627, 1092, 1164
Sau3AI	GATC	3 cut(s)	235, 754, 967
ScaI	AGTACT	2 cut(s)	227, 1057
SchI	GAGTC	3 cut(s)	24, 167, 601
ScrFI	CCNGG	1 cut(s)	276
SduI	GDGCHC	4 cut(s)	11, 124, 445, 1014
SfaNI	GCATC	2 cut(s)	371, 440
SfcI	CTRYAG	1 cut(s)	612
Sfr303I	CCGCGG	1 cut(s)	714
SgrBI	CCGCGG	1 cut(s)	714
SgrDI	CGTCGACG	1 cut(s)	88
SmiMI	CAYNNNNRTG	1 cut(s)	339
Sse9I	AATT	7 cut(s)	155, 457, 485, 596, 701, 743, 1004
SsiI	CCGC	3 cut(s)	426, 711, 713
SspMI	CTAG	1 cut(s)	351
SstI	GAGCTC	2 cut(s)	124, 1014
StyD4I	CCNGG	1 cut(s)	274
TaaI	ACNGT	2 cut(s)	841, 898
TaiI	ACGT	3 cut(s)	95, 188, 1150
TaqI	TCGA	3 cut(s)	89, 411, 679
TasI	AATT	7 cut(s)	155, 457, 485, 596, 701, 743, 1004
TatI	WGTACW	4 cut(s)	31, 225, 642, 1055
TfiI	GAWTC	3 cut(s)	80, 137, 533
Tru1I	TTAA	5 cut(s)	504, 554, 588, 635, 738
Tru9I	TTAA	5 cut(s)	504, 554, 588, 635, 738
TseFI	GTSAC	1 cut(s)	353
TseI	GCWGC	5 cut(s)	317, 428, 626, 1091, 1163
Tsp45I	GTSAC	1 cut(s)	353
TspDTI	ATGAA	4 cut(s)	569, 792, 1017, 1175
TspGWI	ACGGA	2 cut(s)	87, 945
Tth111I	GACNNNGTC	1 cut(s)	64
VspI	ATTAAT	1 cut(s)	588
XapI	RAATTY	1 cut(s)	596
XcmI	CCANNNNNNNNNTGG	1 cut(s)	373
XmiI	GTMKAC	1 cut(s)	89
XspI	CTAG	1 cut(s)	351
ZrmI	AGTACT	2 cut(s)	227, 1057

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.