MD01G1097600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003384
Physical Location & Seq
Forward (+)
21181941 .. 21187305
5365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1097600.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGGCCATTTTGTTTAAGCTTCTGGAGGGAGGGGATTCGACCCAGGTGCCGAATTCGATTGCCTGCAATGAGTTGCTGGTTGCTCTTGGAAAATCTGACATGAGGGTTGAGTTTAAGCAAGTTTTTAATAAGCTTAGAGAGAGCGAGGGGTTTGAGATGGATACTTGGGGGTATAATATTTGCATTCATGTATTTGGGTGTTGGGGTGATTTGGGTACTAGTCTGAGTCTGTTCAGAGAGATGAAAGACTCTAACTTGGATTGCGTCGGGCTGGATTCGTCTACATGTAATAGCCTCATTCATGTGCTCTGCTTGGTGGGGAAGGTGAATGATGCACTTACTGTATGGGAGGAATTGAAGGGCTCCGGCCATGAGCCGGACGCAATTACCTATAGAATTCTCTTATTCAGGGGTGTTGTTAATGATGGCTGCCAGTTTTTTGAGAAAATGGTTCGGAATGGGGTGAGAGCCTCAAGGTGGACGTATACTATTCTCATGGATGGTTTGTTCAGGAATGGAAGGGCGGAGGCTCCGTACACACTATTTTGTGACTTAAAGAAGAAGGGTCAGTTTGTTGATGGTGTAACATATAGCATTGTGGTTTTGCAACTTTGTAAGGATGGTCTGCTTGAGGAAGCGCTAGAATTGGTGGAAGAAATGGAAAGCAGAGGCTTTACTGTTGATTTAATTACTGTATCCTCACTTGTGATTGGGCTGTATAAAGAAGGTCGGTGGGATTGGACGGACAAGCTCATGAAGCACATTAGAGAGGGGAACCTAGTGCCGAGTGTTCTTAAATGGAAGGTTGATATGGAGGCTTCACTGAAAAATCCGCAGAGAAGTAGAAAGGATTATACACCATTGTTCCCAAGTAAAGGTGACTTGAGCGAGATTATGAGTTTAATAAAGTCTGCTGAATCAACAATGGATGCAGACCTAGATTCAGAGGCTGCCAGGGCTAAAGAAGACGATAAGAATTTGTCCACCGATGCGGATCAATGGTCATCATCTCCACATATGGATGAATTGGCTAATCAACTTAAGTCCGCCGACCACTCCTCGCAGCTGTTTTCATTGTCCAGGGGTCTAAGAGTTCAAGCGAAAGGGGAAAGCACTTTTGATATTGACATGGTTAATACTTTTTTGTCCTTATTCTTGGCCAAGGGAAAGCTGAGCATAGCATGCAAATTGTTTGAGATTTTCAGCGACCTTGGTGAAAACCCCGTGAGTTATACTTATAATTCCATGATGAGTTCATTCGTCAAGAAGGGCTACTTCAATGAGACATGGGGTGTACTCAATGAAATGGGAGAAACGCTTGGGGAAGATGGGCAGAGCGGATCTGGCGAGTTCTGTCCTGGATAA

Protein Analysis

455

Amino Acids

50.73

Weight (kDa)

4.88

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 76 - 135 9.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 92 - 135 1.2e-10 PPR repeat family
PPR_2 PF13041 137 - 170 5.6e-06 PPR repeat family
PPR_2 PF13041 161 - 206 9.8e-09 PPR repeat family
PPR_1 PF12854 189 - 221 2.1e-07 PPR repeat
PPR PF01535 195 - 225 1.5e-06 PPR repeat
PPR_2 PF13041 204 - 241 4.5e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1239
AccB1I GGYRCC 1 cut(s) 46
AccBSI CCGCTC 1 cut(s) 1338
AccI GTMKAC 2 cut(s) 281, 485
AciI CCGC 5 cut(s) 524, 833, 992, 1047, 1338
AclWI GGATC 2 cut(s) 1002, 1348
AcoI YGGCCR 3 cut(s) 3, 367, 1158
AcsI RAATTY 3 cut(s) 52, 396, 976
AfaI GTAC 3 cut(s) 217, 536, 1296
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 3 cut(s) 376, 477, 875
AflII CTTAAG 1 cut(s) 1040
AflIII ACRYGT 1 cut(s) 284
AgsI TTSAA 3 cut(s) 358, 1097, 1279
AhlI ACTAGT 1 cut(s) 218
AjnI CCWGG 4 cut(s) 42, 953, 1079, 1357
AloI GAACNNNNNNTCC 2 cut(s) 491, 523
AluBI AGCT 5 cut(s) 19, 133, 751, 1066, 1171
AluI AGCT 5 cut(s) 19, 133, 751, 1066, 1171
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 1 cut(s) 1277
AlwI GGATC 2 cut(s) 1002, 1348
AlwNI CAGNNNCTG 1 cut(s) 950
Aor51HI AGCGCT 1 cut(s) 639
AoxI GGCC 3 cut(s) 3, 367, 1158
ApeKI GCWGC 3 cut(s) 429, 950, 1063
ApoI RAATTY 3 cut(s) 52, 396, 976
AspLEI GCGC 1 cut(s) 640
AsuHPI GGTGA 5 cut(s) 218, 337, 475, 890, 1226
BalI TGGCCA 2 cut(s) 5, 1160
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 972
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 3 cut(s) 416, 937, 1075
BccI CCATC 6 cut(s) 151, 419, 494, 572, 614, 1322
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 4 cut(s) 44, 955, 1081, 1359
BciVI GTATCC 2 cut(s) 154, 706
BcoDI GTCTC 1 cut(s) 1277
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 4 cut(s) 219, 641, 779, 938
BfmI CTRYAG 1 cut(s) 391
BfoI RGCGCY 1 cut(s) 641
BfrI CTTAAG 1 cut(s) 1040
BfuI GTATCC 2 cut(s) 154, 706
BisI GCNGC 3 cut(s) 430, 951, 1064
BlpI GCTNAGC 1 cut(s) 1172
BlsI GCNGC 3 cut(s) 431, 952, 1065
Bme1390I CCNGG 4 cut(s) 44, 955, 1081, 1359
BmiI GGNNCC 4 cut(s) 48, 364, 531, 776
BmrFI CCNGG 4 cut(s) 44, 955, 1081, 1359
BmsI GCATC 3 cut(s) 322, 919, 979
BpiI GAAGAC 1 cut(s) 972
BpmI CTGGAG 1 cut(s) 44
Bpu1102I GCTNAGC 1 cut(s) 1172
BpuEI CTTGAG 3 cut(s) 457, 650, 904
BsaBI GATNNNNATC 1 cut(s) 993
BsaJI CCNNGG 5 cut(s) 42, 954, 1080, 1161, 1210
BsaXI ACNNNNNCTCC 2 cut(s) 518, 548
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 477, 875
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 4 cut(s) 44, 955, 1081, 1359
BseDI CCNNGG 5 cut(s) 42, 954, 1080, 1161, 1210
BseGI GGATG 4 cut(s) 505, 625, 934, 1027
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 3 cut(s) 376, 477, 875
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 2 cut(s) 215, 1163
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 1 cut(s) 1048
BseXI GCAGC 3 cut(s) 416, 937, 1075
BshFI GGCC 3 cut(s) 5, 369, 1160
BshNI GGYRCC 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 2 cut(s) 366, 377
BslI CCNNNNNNNGG 3 cut(s) 376, 477, 875
BsmAI GTCTC 1 cut(s) 1277
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 3 cut(s) 5, 369, 1160
Bsp1286I GDGCHC 2 cut(s) 309, 365
Bsp143I GATC 2 cut(s) 994, 1340
Bsp1720I GCTNAGC 1 cut(s) 1172
BspACI CCGC 5 cut(s) 524, 833, 992, 1047, 1338
BspANI GGCC 3 cut(s) 5, 369, 1160
BspCNI CTCAG 2 cut(s) 216, 1164
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 4 cut(s) 48, 364, 531, 776
BspPI GGATC 2 cut(s) 1002, 1348
BspT107I GGYRCC 1 cut(s) 46
BspTI CTTAAG 1 cut(s) 1040
BsrBI CCGCTC 1 cut(s) 1338
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 5 cut(s) 42, 954, 1080, 1161, 1210
BssMI GATC 2 cut(s) 994, 1340
BssNAI GTATAC 1 cut(s) 486
BssT1I CCWWGG 2 cut(s) 1161, 1210
Bst1107I GTATAC 1 cut(s) 486
Bst2UI CCWGG 4 cut(s) 44, 955, 1081, 1359
Bst4CI ACNGT 3 cut(s) 343, 679, 694
BstAFI CTTAAG 1 cut(s) 1040
BstAPI GCANNNNNTGC 1 cut(s) 1182
BstC8I GCNNGC 2 cut(s) 64, 1183
BstDEI CTNAG 4 cut(s) 134, 224, 1088, 1172
BstF5I GGATG 4 cut(s) 505, 625, 934, 1027
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 1 cut(s) 640
BstKTI GATC 2 cut(s) 997, 1343
BstMAI GTCTC 1 cut(s) 1277
BstMBI GATC 2 cut(s) 994, 1340
BstMWI GCNNNNNNNGC 4 cut(s) 757, 956, 1182, 1344
BstNI CCWGG 4 cut(s) 44, 955, 1081, 1359
BstNSI RCATGY 2 cut(s) 288, 1185
BstSCI CCNGG 4 cut(s) 42, 953, 1079, 1357
BstSFI CTRYAG 1 cut(s) 391
BstV1I GCAGC 3 cut(s) 416, 937, 1075
BstV2I GAAGAC 1 cut(s) 972
BstX2I RGATCY 1 cut(s) 1340
BstYI RGATCY 1 cut(s) 1340
BstZ17I GTATAC 1 cut(s) 486
BsuI GTATCC 2 cut(s) 154, 706
BsuRI GGCC 3 cut(s) 5, 369, 1160
BtsCI GGATG 4 cut(s) 505, 625, 934, 1027
BtsIMutI CAGTG 1 cut(s) 821
Cac8I GCNNGC 2 cut(s) 64, 1183
CaiI CAGNNNCTG 1 cut(s) 950
CciI TCATGA 1 cut(s) 753
CfoI GCGC 1 cut(s) 640
CseI GACGC 2 cut(s) 253, 389
Csp6I GTAC 3 cut(s) 216, 535, 1295
CviQI GTAC 3 cut(s) 216, 535, 1295
DdeI CTNAG 4 cut(s) 134, 224, 1088, 1172
DpnI GATC 2 cut(s) 996, 1342
DpnII GATC 2 cut(s) 994, 1340
EaeI YGGCCR 3 cut(s) 3, 367, 1158
EciI GGCGGA 2 cut(s) 539, 1036
Eco130I CCWWGG 2 cut(s) 1161, 1210
Eco24I GRGCYC 1 cut(s) 365
Eco47III AGCGCT 1 cut(s) 639
EcoRI GAATTC 2 cut(s) 52, 396
EcoRII CCWGG 4 cut(s) 42, 953, 1079, 1357
EcoT14I CCWWGG 2 cut(s) 1161, 1210
EcoT38I GRGCYC 1 cut(s) 365
ErhI CCWWGG 2 cut(s) 1161, 1210
FalI AAGNNNNNCTT 2 cut(s) 1259, 1291
FauNDI CATATG 1 cut(s) 1017
FblI GTMKAC 2 cut(s) 281, 485
Fnu4HI GCNGC 3 cut(s) 430, 951, 1064
FokI GGATG 4 cut(s) 512, 632, 941, 1034
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 3 cut(s) 430, 951, 1064
FspBI CTAG 4 cut(s) 219, 641, 779, 938
GlaI GCGC 1 cut(s) 639
GluI GCNGC 3 cut(s) 430, 951, 1064
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 641
HaeIII GGCC 3 cut(s) 5, 369, 1160
HapII CCGG 2 cut(s) 366, 377
HgaI GACGC 2 cut(s) 253, 389
HhaI GCGC 1 cut(s) 640
Hin6I GCGC 1 cut(s) 638
HinP1I GCGC 1 cut(s) 638
HindIII AAGCTT 2 cut(s) 17, 131
HinfI GANTC 6 cut(s) 35, 226, 248, 275, 917, 941
HpaII CCGG 2 cut(s) 366, 377
HphI GGTGA 5 cut(s) 218, 337, 475, 890, 1226
Hpy166II GTNNAC 6 cut(s) 282, 480, 486, 537, 984, 1295
Hpy188I TCNGA 5 cut(s) 97, 225, 236, 456, 946
Hpy188III TCNNGA 4 cut(s) 23, 511, 754, 1264
Hpy8I GTNNAC 6 cut(s) 282, 480, 486, 537, 984, 1295
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 7 cut(s) 316, 352, 513, 556, 719, 796, 1261
HpyCH4III ACNGT 3 cut(s) 343, 679, 694
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 6 cut(s) 66, 183, 335, 607, 932, 1185
HpyF10VI GCNNNNNNNGC 4 cut(s) 757, 956, 1182, 1344
HpyF3I CTNAG 4 cut(s) 134, 224, 1088, 1172
HpySE526I ACGT 1 cut(s) 482
HspAI GCGC 1 cut(s) 638
Kzo9I GATC 2 cut(s) 994, 1340
LmnI GCTCC 2 cut(s) 368, 535
Lsp1109I GCAGC 3 cut(s) 416, 937, 1075
LweI GCATC 3 cut(s) 322, 919, 979
MaeI CTAG 4 cut(s) 219, 641, 779, 938
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 3 cut(s) 548, 583, 878
MalI GATC 2 cut(s) 996, 1342
MbiI CCGCTC 1 cut(s) 1338
MboI GATC 2 cut(s) 994, 1340
MboII GAAGA 4 cut(s) 571, 665, 977, 1337
MflI RGATCY 1 cut(s) 1340
MhlI GDGCHC 2 cut(s) 309, 365
MlsI TGGCCA 2 cut(s) 5, 1160
MluNI TGGCCA 2 cut(s) 5, 1160
MlyI GAGTC 2 cut(s) 235, 242
Mox20I TGGCCA 2 cut(s) 5, 1160
MscI TGGCCA 2 cut(s) 5, 1160
MslI CAYNNNNRTG 2 cut(s) 302, 1020
Msp20I TGGCCA 2 cut(s) 5, 1160
MspA1I CMGCKG 1 cut(s) 1066
MspCI CTTAAG 1 cut(s) 1040
MspI CCGG 2 cut(s) 366, 377
MspR9I CCNGG 4 cut(s) 44, 955, 1081, 1359
Mva1269I GAATGC 1 cut(s) 183
MvaI CCWGG 4 cut(s) 44, 955, 1081, 1359
MwoI GCNNNNNNNGC 4 cut(s) 757, 956, 1182, 1344
NdeI CATATG 1 cut(s) 1017
NdeII GATC 2 cut(s) 994, 1340
NlaIV GGNNCC 4 cut(s) 48, 364, 531, 776
NmeAIII GCCGAG 1 cut(s) 810
NmuCI GTSAC 2 cut(s) 548, 878
NspI RCATGY 2 cut(s) 288, 1185
PaeI GCATGC 1 cut(s) 1185
PagI TCATGA 1 cut(s) 753
PciI ACATGT 1 cut(s) 284
PctI GAATGC 1 cut(s) 183
PfeI GAWTC 4 cut(s) 35, 275, 917, 941
PfoI TCCNGGA 1 cut(s) 1357
PkrI GCNGC 3 cut(s) 431, 952, 1065
PleI GAGTC 2 cut(s) 234, 242
PpsI GAGTC 2 cut(s) 234, 242
PscI ACATGT 1 cut(s) 284
PsiI TTATAA 1 cut(s) 1239
Psp6I CCWGG 4 cut(s) 42, 953, 1079, 1357
PspGI CCWGG 4 cut(s) 42, 953, 1079, 1357
PspN4I GGNNCC 4 cut(s) 48, 364, 531, 776
PstNI CAGNNNCTG 1 cut(s) 950
PsuI RGATCY 1 cut(s) 1340
PvuII CAGCTG 1 cut(s) 1066
RsaI GTAC 3 cut(s) 217, 536, 1296
RsaNI GTAC 3 cut(s) 216, 535, 1295
RseI CAYNNNNRTG 2 cut(s) 302, 1020
SatI GCNGC 3 cut(s) 430, 951, 1064
Sau3AI GATC 2 cut(s) 994, 1340
SchI GAGTC 2 cut(s) 235, 242
ScrFI CCNGG 4 cut(s) 44, 955, 1081, 1359
SduI GDGCHC 2 cut(s) 309, 365
SfaNI GCATC 3 cut(s) 322, 919, 979
SfcI CTRYAG 1 cut(s) 391
SmiMI CAYNNNNRTG 2 cut(s) 302, 1020
SmlI CTYRAG 4 cut(s) 472, 629, 883, 1040
SmoI CTYRAG 4 cut(s) 472, 629, 883, 1040
SpeI ACTAGT 1 cut(s) 218
SphI GCATGC 1 cut(s) 1185
SsiI CCGC 5 cut(s) 524, 833, 992, 1047, 1338
SspI AATATT 1 cut(s) 178
SspMI CTAG 4 cut(s) 219, 641, 779, 938
StyD4I CCNGG 4 cut(s) 42, 953, 1079, 1357
StyI CCWWGG 2 cut(s) 1161, 1210
TaaI ACNGT 3 cut(s) 343, 679, 694
TaiI ACGT 1 cut(s) 485
TaqI TCGA 2 cut(s) 38, 56
TatI WGTACW 1 cut(s) 1294
TfiI GAWTC 4 cut(s) 35, 275, 917, 941
TscAI CASTG 1 cut(s) 828
TseFI GTSAC 2 cut(s) 548, 878
TseI GCWGC 3 cut(s) 429, 950, 1063
Tsp45I GTSAC 2 cut(s) 548, 878
TspDTI ATGAA 8 cut(s) 176, 257, 290, 770, 1038, 1062, 1245, 1317
TspGWI ACGGA 2 cut(s) 522, 758
TspRI CASTG 1 cut(s) 828
Vha464I CTTAAG 1 cut(s) 1040
XapI RAATTY 3 cut(s) 52, 396, 976
XceI RCATGY 2 cut(s) 288, 1185
XmiI GTMKAC 2 cut(s) 281, 485
XspI CTAG 4 cut(s) 219, 641, 779, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.