Rh1BG265000

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003384
Physical Location & Seq
Forward (+)
39940350 .. 39942395
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG265000.1

Sequence Viewer

Length: 1836 bp
ATGAAACAGCATGCTGTAATAGTCGATTCCGGAACCTTCAAGTCTTTGCTGGACGCCTTCATAAGGGCAGGTAAGTTTGACATGGCAATCGAAATTTTAGACATTATGCAAGAACTGGGTGCTGAGCTGAGTACGGATATGTACAACTCGGTGTTGGTTGCTTTGGTTAGGAAGGGTCAGTTGGGTTTGGCTATGTCTATTTTGGTTAAGCTTCTAGAAGGTGGGTCTTCTCAAGTACCCAATTGCATTGCCTGTAATGAATTGCTGGTGGGTCTTAGGAAAGGTGACATGAGGGTAGAGTTTAAGCAGGTGTTTGATAAGCTTAGGGGGAATGAGTGGTTTGAGATGGATACTTGGGGTTATAATATTTGTATTCATGCGTTTGGGTGTTGGGGTGATCTGGGTACTAGTTTGAGTTTATTTAAGGAGATGAAAGACTTGAATTCGGACTCGGTTTTTCCTGATTTGTCTACTTATAATAGCCTCATTCATGTGCTGTGTTTGGTTGGGAAGGTGGATGATGCAGTTACTGTGTGGGAGGAGTTGAAGTGTTCTGGTCATGAGCCGGATGCCATTACATACCGGATTCTTATTCAGGGCTGTTGTAAGTGTTATCGAATAGAGGAAGCTACCAAGATTTTTAGTGAAATGCAGAACAATGGGTACAATCCGGATACGGTTGTTTATAATTCGTTGATGGATGGGTTGTTCAAGGCGAGGAAAGTTAATGAAGGGTGTCAGATGTTTGAGAGGATGGTTCAGTATGGGGTGAGAGCCTCGACGTGGACGTATAATATTCTGATTGATGGGTTGTTTAGGAATGCAAGGGCCGAGGCTGCTTACACTATATTTTGTGACTTGAAGAAGAAAGGTCAGTTTGTGGATGGTGTTACGTATAGCATTGTTGCTCTGCAACTTTGTAGGGAGGGTCTGCTCGAGGAGGCGCTAGAATTGGTGGAAGAAATGGAAATGCGAGGCCTTACTGTTGATTTGGTTACTACATCAACACTCATGATTGGGCTGTATAAGCATAGTCGATGGGATTGGACAGACAAGCTCATGAAGCGTATTAGGGATGGTAATCTGCTGCCTAGTGTTCTTAGATGGAAAGTCGATATGGAAGCTACAATGAAAGCTCCACAGAGCAAGAAAAAGGACCTTACACCACTGTTCCCAAGTAAAGGTGATTTCAGCGACGTTTTGAGTCTAATAAGTTCTGCCGCCTCGAAAATGGATGGAGTCATTGAATCAGATGATGCTGGGGTTAAAGATGATAATAATTCATCTACAGATATTGATCAGTGGTCATCATCTCCACATATGGATCACTTAGCCAATCAATTGAAGTCCACTGACCAAGCCTCTCAGCTGTTTTCGCTATCTAGAGGGCAACGAGTTCAAGCCAAAGGAGAAAGCACTTTTGATATTGATATGGTAAATACTTTCTTATCCCTATTTTTAGCCAAGGGGAAGCTGAGCATAGCTTGCAAATTGTTTGAGATTTTCAGTGATGCGGGTGCAAACCCTGTGAGTTACACTTATAATTCCATCTTGAGTTCGTTTGTCAAGAAGGGCTACTTCAATGAGGCATGGGGTGTGCTCAGTGAAATGGGAGAAAAAGTTTGCCCCACAGATATAGCAACATACAATATGATAATTCAAGGCTTGGGGAAGATGGGAAGGGCCGATCTAGCCAGTAGTGTTCTGGATAAGCTAATGAAGCAGGGCTGCACACCAAACCATGTTACTGACACCACTTTGGATTTTCTGGGGAAGGAGATTGAGAAATCGAGGTACCAAAAGGCATCATTTGTACGAAATAAGGATGACTCCTGA

Protein Analysis

611

Amino Acids

68.32

Weight (kDa)

5.32

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 132 - 290 5.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 145 - 201 3.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 152 - 182 4.4e-09 PPR repeat
PPR_2 PF13041 154 - 203 2.9e-16 PPR repeat family
PPR_1 PF12854 186 - 218 5.2e-10 PPR repeat
PPR PF01535 192 - 221 1.2e-06 PPR repeat
PPR_2 PF13041 196 - 227 3.6e-06 PPR repeat family
PPR_3 PF13812 212 - 269 8e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 220 - 253 9.6e-08 PPR repeat
PPR_2 PF13041 224 - 272 1.9e-14 PPR repeat family
PPR PF01535 227 - 257 9.2e-06 PPR repeat
PPR_2 PF13041 262 - 302 3.9e-09 PPR repeat family
PPR_2 PF13041 306 - 343 3.3e-06 PPR repeat family
PPR_2 PF13041 510 - 558 7.7e-12 PPR repeat family
PPR_2 PF13041 545 - 587 1.1e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 363, 477, 687, 1542
AarI CACCTGC 1 cut(s) 298
Acc36I ACCTGC 2 cut(s) 59, 298
Acc65I GGTACC 1 cut(s) 1794
AccB1I GGYRCC 1 cut(s) 1794
AccI GTMKAC 1 cut(s) 470
AccIII TCCGGA 2 cut(s) 29, 670
AciI CCGC 2 cut(s) 1221, 1514
AclWI GGATC 1 cut(s) 1332
AcsI RAATTY 2 cut(s) 93, 442
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 7 cut(s) 133, 143, 237, 406, 665, 1796, 1815
AfiI CCNNNNNNNGG 3 cut(s) 63, 783, 1181
AhlI ACTAGT 1 cut(s) 407
AjiI CACGTC 1 cut(s) 783
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AloI GAACNNNNNNTCC 2 cut(s) 692, 724
Alw21I GWGCWC 1 cut(s) 1602
AlwI GGATC 1 cut(s) 1332
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 935
Aor13HI TCCGGA 2 cut(s) 29, 670
AoxI GGCC 3 cut(s) 828, 976, 1683
ApeKI GCWGC 3 cut(s) 836, 1087, 1728
ApoI RAATTY 2 cut(s) 93, 442
Asp718I GGTACC 1 cut(s) 1794
AspLEI GCGC 1 cut(s) 946
AspS9I GGNCC 3 cut(s) 828, 1156, 1683
AsuHPI GGTGA 4 cut(s) 296, 407, 781, 1196
AvaI CYCGRG 1 cut(s) 935
AvaII GGWCC 1 cut(s) 1156
BaeI ACNNNNGTAYC 2 cut(s) 666, 699
BanI GGYRCC 1 cut(s) 1794
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 1602
BbvI GCAGC 3 cut(s) 823, 1074, 1715
BciVI GTATCC 2 cut(s) 343, 667
BclI TGATCA 1 cut(s) 1297
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 6 cut(s) 215, 408, 947, 1092, 1383, 1691
BfmI CTRYAG 1 cut(s) 1287
BfoI RGCGCY 1 cut(s) 947
BfuAI ACCTGC 2 cut(s) 59, 298
BfuI GTATCC 2 cut(s) 343, 667
BisI GCNGC 4 cut(s) 837, 1088, 1221, 1729
BlpI GCTNAGC 2 cut(s) 123, 1475
BlsI GCNGC 4 cut(s) 838, 1089, 1222, 1730
Bme18I GGWCC 1 cut(s) 1156
BmeT110I CYCGRG 1 cut(s) 935
BmgBI CACGTC 1 cut(s) 783
BmgT120I GGNCC 3 cut(s) 828, 1156, 1683
BmiI GGNNCC 2 cut(s) 34, 1796
BmrI ACTGGG 1 cut(s) 125
BmsI GCATC 5 cut(s) 511, 559, 1246, 1501, 1814
BmuI ACTGGG 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 323
Bpu1102I GCTNAGC 2 cut(s) 123, 1475
BpuEI CTTGAG 2 cut(s) 216, 1573
BsaAI YACGTR 1 cut(s) 894
BsaBI GATNNNNATC 2 cut(s) 1080, 1296
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 831, 1464
BsaWI WCCGGW 3 cut(s) 29, 582, 670
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 1605, 1635
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 783, 1181
Bse1I ACTGG 2 cut(s) 120, 1695
Bse3DI GCAATG 1 cut(s) 246
Bse8I GATNNNNATC 2 cut(s) 1080, 1296
BseAI TCCGGA 2 cut(s) 29, 670
BseDI CCNNGG 2 cut(s) 831, 1464
BseGI GGATG 8 cut(s) 523, 574, 706, 759, 889, 1081, 1240, 1831
BseJI GATNNNNATC 2 cut(s) 1080, 1296
BseLI CCNNNNNNNGG 3 cut(s) 63, 783, 1181
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 5 cut(s) 114, 119, 1379, 1466, 1615
BseNI ACTGG 2 cut(s) 120, 1695
BseRI GAGGAG 2 cut(s) 554, 953
BseXI GCAGC 3 cut(s) 823, 1074, 1715
BseYI CCCAGC 1 cut(s) 1259
BsgI GTGCAG 1 cut(s) 1714
BshFI GGCC 3 cut(s) 830, 978, 1685
BshNI GGYRCC 1 cut(s) 1794
BsiHKAI GWGCWC 1 cut(s) 1602
BsiHKCI CYCGRG 1 cut(s) 935
BsiSI CCGG 4 cut(s) 30, 566, 583, 671
BslI CCNNNNNNNGG 3 cut(s) 63, 783, 1181
BsmI GAATGC 1 cut(s) 826
BsnI GGCC 3 cut(s) 830, 978, 1685
BsoBI CYCGRG 1 cut(s) 935
Bsp1286I GDGCHC 1 cut(s) 1602
Bsp13I TCCGGA 2 cut(s) 29, 670
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 397, 1297, 1324, 1687
Bsp1720I GCTNAGC 2 cut(s) 123, 1475
BspACI CCGC 2 cut(s) 1221, 1514
BspANI GGCC 3 cut(s) 830, 978, 1685
BspCNI CTCAG 5 cut(s) 115, 120, 1378, 1467, 1614
BspEI TCCGGA 2 cut(s) 29, 670
BspHI TCATGA 3 cut(s) 559, 1011, 1059
BspLI GGNNCC 2 cut(s) 34, 1796
BspMI ACCTGC 2 cut(s) 59, 298
BspPI GGATC 1 cut(s) 1332
BspT107I GGYRCC 1 cut(s) 1794
BsrDI GCAATG 1 cut(s) 246
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 2 cut(s) 120, 1695
BssECI CCNNGG 2 cut(s) 831, 1464
BssMI GATC 4 cut(s) 397, 1297, 1324, 1687
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 1464
Bst4CI ACNGT 4 cut(s) 532, 679, 985, 1170
BstACI GRCGYC 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 1485
BstAUI TGTACA 1 cut(s) 141
BstBAI YACGTR 1 cut(s) 894
BstC8I GCNNGC 2 cut(s) 12, 1486
BstDEI CTNAG 9 cut(s) 123, 128, 275, 323, 1100, 1330, 1365, 1475, 1601
BstENI CCTNNNNNAGG 1 cut(s) 61
BstF5I GGATG 8 cut(s) 523, 574, 706, 759, 889, 1081, 1240, 1831
BstH2I RGCGCY 1 cut(s) 947
BstHHI GCGC 1 cut(s) 946
BstKTI GATC 4 cut(s) 400, 1300, 1327, 1690
BstMBI GATC 4 cut(s) 397, 1297, 1324, 1687
BstMWI GCNNNNNNNGC 7 cut(s) 836, 1027, 1063, 1375, 1485, 1691, 1720
BstNSI RCATGY 1 cut(s) 14
BstSFI CTRYAG 1 cut(s) 1287
BstSNI TACGTA 1 cut(s) 894
BstV1I GCAGC 3 cut(s) 823, 1074, 1715
BstV2I GAAGAC 1 cut(s) 219
BsuI GTATCC 2 cut(s) 343, 667
BsuRI GGCC 3 cut(s) 830, 978, 1685
BtrI CACGTC 1 cut(s) 783
BtsCI GGATG 8 cut(s) 523, 574, 706, 759, 889, 1081, 1240, 1831
BtsIMutI CAGTG 5 cut(s) 1166, 1307, 1350, 1513, 1609
BveI ACCTGC 2 cut(s) 59, 298
Cac8I GCNNGC 2 cut(s) 12, 1486
CaiI CAGNNNCTG 1 cut(s) 530
CciI TCATGA 3 cut(s) 559, 1011, 1059
CfoI GCGC 1 cut(s) 946
Cfr13I GGNCC 3 cut(s) 828, 1156, 1683
CseI GACGC 1 cut(s) 62
Csp6I GTAC 7 cut(s) 132, 142, 236, 405, 664, 1795, 1814
CviQI GTAC 7 cut(s) 132, 142, 236, 405, 664, 1795, 1814
DdeI CTNAG 9 cut(s) 123, 128, 275, 323, 1100, 1330, 1365, 1475, 1601
DpnI GATC 4 cut(s) 399, 1299, 1326, 1689
DpnII GATC 4 cut(s) 397, 1297, 1324, 1687
Eco105I TACGTA 1 cut(s) 894
Eco130I CCWWGG 1 cut(s) 1464
Eco147I AGGCCT 1 cut(s) 978
Eco47I GGWCC 1 cut(s) 1156
Eco88I CYCGRG 1 cut(s) 935
EcoNI CCTNNNNNAGG 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 1156
EcoRI GAATTC 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 1464
ErhI CCWWGG 1 cut(s) 1464
FalI AAGNNNNNCTT 2 cut(s) 1562, 1594
FauI CCCGC 1 cut(s) 1507
FauNDI CATATG 1 cut(s) 1320
FbaI TGATCA 1 cut(s) 1297
FblI GTMKAC 1 cut(s) 470
Fnu4HI GCNGC 4 cut(s) 837, 1088, 1221, 1729
FokI GGATG 7 cut(s) 530, 581, 713, 766, 896, 1088, 1247
Fsp4HI GCNGC 4 cut(s) 837, 1088, 1221, 1729
FspBI CTAG 6 cut(s) 215, 408, 947, 1092, 1383, 1691
GlaI GCGC 1 cut(s) 945
GluI GCNGC 4 cut(s) 837, 1088, 1221, 1729
GsaI CCCAGC 1 cut(s) 1263
HaeII RGCGCY 1 cut(s) 947
HaeIII GGCC 3 cut(s) 830, 978, 1685
HapII CCGG 4 cut(s) 30, 566, 583, 671
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 946
Hin1I GRCGYC 1 cut(s) 54
Hin6I GCGC 1 cut(s) 944
HinP1I GCGC 1 cut(s) 944
HindIII AAGCTT 2 cut(s) 209, 320
HinfI GANTC 7 cut(s) 26, 449, 586, 1204, 1239, 1247, 1829
HpaII CCGG 4 cut(s) 30, 566, 583, 671
HphI GGTGA 4 cut(s) 296, 407, 781, 1196
Hpy166II GTNNAC 3 cut(s) 471, 786, 1350
Hpy188I TCNGA 4 cut(s) 448, 741, 801, 1252
Hpy8I GTNNAC 3 cut(s) 471, 786, 1350
Hpy99I CGWCG 2 cut(s) 784, 1199
HpyAV CCTTC 9 cut(s) 46, 67, 166, 212, 505, 725, 1564, 1674, 1768
HpyCH4III ACNGT 4 cut(s) 532, 679, 985, 1170
HpyCH4IV ACGT 4 cut(s) 782, 788, 893, 1197
HpyCH4V TGCA 9 cut(s) 109, 246, 524, 652, 824, 913, 1488, 1520, 1731
HpyF10VI GCNNNNNNNGC 7 cut(s) 836, 1027, 1063, 1375, 1485, 1691, 1720
HpyF3I CTNAG 9 cut(s) 123, 128, 275, 323, 1100, 1330, 1365, 1475, 1601
HpySE526I ACGT 4 cut(s) 782, 788, 893, 1197
Hsp92I GRCGYC 1 cut(s) 54
HspAI GCGC 1 cut(s) 944
Kpn2I TCCGGA 2 cut(s) 29, 670
KpnI GGTACC 1 cut(s) 1798
Ksp22I TGATCA 1 cut(s) 1297
Kzo9I GATC 4 cut(s) 397, 1297, 1324, 1687
LmnI GCTCC 1 cut(s) 1141
Lsp1109I GCAGC 3 cut(s) 823, 1074, 1715
LweI GCATC 5 cut(s) 511, 559, 1246, 1501, 1814
MaeI CTAG 6 cut(s) 215, 408, 947, 1092, 1383, 1691
MaeII ACGT 4 cut(s) 782, 788, 893, 1197
MaeIII GTNAC 7 cut(s) 284, 526, 854, 889, 994, 1532, 1744
MalI GATC 4 cut(s) 399, 1299, 1326, 1689
MboI GATC 4 cut(s) 397, 1297, 1324, 1687
MboII GAAGA 5 cut(s) 219, 874, 877, 971, 1684
MfeI CAATTG 2 cut(s) 241, 1340
MhlI GDGCHC 1 cut(s) 1602
MlyI GAGTC 4 cut(s) 443, 1213, 1248, 1823
MroI TCCGGA 2 cut(s) 29, 670
MseI TTAA 5 cut(s) 207, 303, 423, 726, 1266
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 1369
MspI CCGG 4 cut(s) 30, 566, 583, 671
MunI CAATTG 2 cut(s) 241, 1340
Mva1269I GAATGC 1 cut(s) 826
MwoI GCNNNNNNNGC 7 cut(s) 836, 1027, 1063, 1375, 1485, 1691, 1720
NdeI CATATG 1 cut(s) 1320
NdeII GATC 4 cut(s) 397, 1297, 1324, 1687
NlaIV GGNNCC 2 cut(s) 34, 1796
NmeAIII GCCGAG 1 cut(s) 856
NmuCI GTSAC 2 cut(s) 284, 854
NspI RCATGY 1 cut(s) 14
PaeI GCATGC 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 935
PagI TCATGA 3 cut(s) 559, 1011, 1059
PaqCI CACCTGC 1 cut(s) 298
PceI AGGCCT 1 cut(s) 978
PcsI WCGNNNNNNNCGW 1 cut(s) 785
PctI GAATGC 1 cut(s) 826
PfeI GAWTC 3 cut(s) 26, 586, 1247
PkrI GCNGC 4 cut(s) 838, 1089, 1222, 1730
PleI GAGTC 4 cut(s) 443, 1212, 1247, 1823
PpsI GAGTC 4 cut(s) 443, 1212, 1247, 1823
Ppu21I YACGTR 1 cut(s) 894
PpuMI RGGWCCY 1 cut(s) 1156
PsiI TTATAA 4 cut(s) 363, 477, 687, 1542
Psp5II RGGWCCY 1 cut(s) 1156
PspFI CCCAGC 1 cut(s) 1259
PspN4I GGNNCC 2 cut(s) 34, 1796
PspPI GGNCC 3 cut(s) 828, 1156, 1683
PspPPI RGGWCCY 1 cut(s) 1156
PspXI VCTCGAGB 1 cut(s) 935
PsrI GAACNNNNNNTAC 2 cut(s) 647, 679
PstNI CAGNNNCTG 1 cut(s) 530
PvuII CAGCTG 1 cut(s) 1369
RsaI GTAC 7 cut(s) 133, 143, 237, 406, 665, 1796, 1815
RsaNI GTAC 7 cut(s) 132, 142, 236, 405, 664, 1795, 1814
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 5 cut(s) 207, 303, 423, 726, 1266
SatI GCNGC 4 cut(s) 837, 1088, 1221, 1729
Sau3AI GATC 4 cut(s) 397, 1297, 1324, 1687
Sau96I GGNCC 3 cut(s) 828, 1156, 1683
SchI GAGTC 4 cut(s) 443, 1213, 1248, 1823
SduI GDGCHC 1 cut(s) 1602
SfaNI GCATC 5 cut(s) 511, 559, 1246, 1501, 1814
SfcI CTRYAG 1 cut(s) 1287
Sfr274I CTCGAG 1 cut(s) 935
SinI GGWCC 1 cut(s) 1156
SlaI CTCGAG 1 cut(s) 935
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 3 cut(s) 231, 935, 1552
SmoI CTYRAG 3 cut(s) 231, 935, 1552
SnaBI TACGTA 1 cut(s) 894
SpeI ACTAGT 1 cut(s) 407
SphI GCATGC 1 cut(s) 14
SseBI AGGCCT 1 cut(s) 978
SsiI CCGC 2 cut(s) 1221, 1514
SspI AATATT 2 cut(s) 367, 796
SspMI CTAG 6 cut(s) 215, 408, 947, 1092, 1383, 1691
StuI AGGCCT 1 cut(s) 978
StyI CCWWGG 1 cut(s) 1464
TaaI ACNGT 4 cut(s) 532, 679, 985, 1170
TaiI ACGT 4 cut(s) 785, 791, 896, 1200
TaqI TCGA 9 cut(s) 24, 90, 616, 779, 936, 1036, 1113, 1226, 1790
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 1223
TfiI GAWTC 3 cut(s) 26, 586, 1247
Tru1I TTAA 5 cut(s) 207, 303, 423, 726, 1266
Tru9I TTAA 5 cut(s) 207, 303, 423, 726, 1266
TscAI CASTG 5 cut(s) 1173, 1307, 1357, 1513, 1609
TseFI GTSAC 2 cut(s) 284, 854
TseI GCWGC 3 cut(s) 836, 1087, 1728
Tsp45I GTSAC 2 cut(s) 284, 854
TspGWI ACGGA 1 cut(s) 149
TspRI CASTG 5 cut(s) 1173, 1307, 1357, 1513, 1609
VpaK11BI GGWCC 1 cut(s) 1156
XagI CCTNNNNNAGG 1 cut(s) 61
XapI RAATTY 2 cut(s) 93, 442
XbaI TCTAGA 2 cut(s) 214, 1382
XceI RCATGY 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 1 cut(s) 1702
XhoI CTCGAG 1 cut(s) 935
XmiI GTMKAC 1 cut(s) 470
XspI CTAG 6 cut(s) 215, 408, 947, 1092, 1383, 1691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.